RLG00000028390

pfkB family carbohydrate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003570
Physical Location & Seq
Reverse (-)
22136273 .. 22138152
1880 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028390

Sequence Viewer

Length: 945 bp
ATGTTGATTGATTTTGTCCCAACGAGTGCTGGAGTTTCTCTTGCTGATGCAGAAGGTTTCAAGAAAGCTGCTGGTGGAGCTCCTGCTAATGTTGCAGTTGGAATCTCAAGATTAGGAGGCTCAGCAGCTTTTATGGGCAAGGTTGGTGCAGATGAGTTTGGATACATGCTGTCTAATATTCTGAAGGAAAACAATGTGGACAATTCCGGCATGTGTTTTGACCAACATGCAAGGACTGCATTGGCTTTTGTTACACTGAGAAGTGATGGAGAACGCGAATTCATGTTCTATCGCAACCCGAGTGCTGATATGCTTCTCACTAAGGAAGAACTTAACGTCAACATAATCAAAAAGGCAAATATATTTCACTATGGGTCGATTAGTCTAATTGATGAACCATGTAAATCAGCACATCTCGCTGCGATGGACATTGCTAAGAAGGCTGGCTGTATCCTCTCTTATGACCCGAACTTGAGATTGCCACTGTGGCCTTCTGCAGAGGCTGCTCAGAAGGGCATTATGAGCATTTGGAACCTAGCTGACATTATTAAAATAAGTGAGGAGGAAATTACATTTTTGACTGGAGGTGATGATCCTTATGATGATGATGTGGTCTTGAACAAGCTTTATCACCCCAATCTGAAGCTTCTGTTAGTAACTGAAGGGCCAGCAGGCTGCAGATACTACACAAAGGAATTCAAGGGTAAGGTTGCAGGAATCAAAGCTGACTGTGTTGATACAACTGGAGCTGGTGACTCTTTTGTTGGCTCTGTACTCAACAGCTTGGCTTCAGACCCTGATTTGTTCAAGAATGAACAACGGTTGAAGGAGGCTCTCAAATTTGCCAATGCTTGTGGTGCTATCACTGTGGAAAAGAAAGGAGCCATTCCTGCCCTACCCACAAAAGAAGCTGTCCATAAAGTTCTCAGCCAAACATCGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00500 ko00520 ko01100 map00051 map00500 map00520 map01100
Pfam Domains
Protein Families
PfkB

Protein Analysis

315

Amino Acids

33.68

Weight (kDa)

5.49

Isoelectric Point (pI)

29.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 2 - 301 2.2e-72 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 276
AclWI GGATC 1 cut(s) 587
AcsI RAATTY 3 cut(s) 278, 695, 839
AcuI CTGAAG 4 cut(s) 203, 662, 681, 774
AfaI GTAC 1 cut(s) 774
AfiI CCNNNNNNNGG 1 cut(s) 937
AgsI TTSAA 5 cut(s) 61, 619, 700, 808, 826
Alw21I GWGCWC 1 cut(s) 82
AlwI GGATC 1 cut(s) 587
AlwNI CAGNNNCTG 2 cut(s) 503, 797
Ama87I CYCGRG 1 cut(s) 298
AoxI GGCC 2 cut(s) 488, 665
ApeKI GCWGC 5 cut(s) 68, 125, 419, 503, 675
ApoI RAATTY 3 cut(s) 278, 695, 839
AspS9I GGNCC 1 cut(s) 665
AsuHPI GGTGA 3 cut(s) 599, 623, 764
AvaI CYCGRG 1 cut(s) 298
BanII GRGCYC 1 cut(s) 82
Bbv12I GWGCWC 1 cut(s) 82
BbvI GCAGC 5 cut(s) 55, 137, 406, 490, 662
BccI CCATC 2 cut(s) 260, 418
BciVI GTATCC 2 cut(s) 155, 461
BfaI CTAG 1 cut(s) 536
BfmI CTRYAG 2 cut(s) 495, 676
BfuI GTATCC 2 cut(s) 155, 461
BglI GCCNNNNNGGC 1 cut(s) 487
BisI GCNGC 5 cut(s) 69, 126, 420, 504, 676
BlpI GCTNAGC 1 cut(s) 121
BlsI GCNGC 5 cut(s) 70, 127, 421, 505, 677
BmeT110I CYCGRG 1 cut(s) 298
BmgT120I GGNCC 1 cut(s) 665
BmiI GGNNCC 2 cut(s) 533, 883
BmsI GCATC 1 cut(s) 37
BpmI CTGGAG 3 cut(s) 51, 603, 765
Bpu1102I GCTNAGC 1 cut(s) 121
BpuEI CTTGAG 2 cut(s) 91, 493
Bsc4I CCNNNNNNNGG 1 cut(s) 937
Bse1I ACTGG 2 cut(s) 586, 748
Bse3DI GCAATG 1 cut(s) 429
BseLI CCNNNNNNNGG 1 cut(s) 937
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 4 cut(s) 135, 248, 521, 940
BseNI ACTGG 2 cut(s) 586, 748
BseRI GAGGAG 1 cut(s) 575
BseXI GCAGC 5 cut(s) 55, 137, 406, 490, 662
BsgI GTGCAG 1 cut(s) 168
Bsh1236I CGCG 1 cut(s) 276
BshFI GGCC 2 cut(s) 490, 667
BsiHKAI GWGCWC 1 cut(s) 82
BsiHKCI CYCGRG 1 cut(s) 298
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 1 cut(s) 2
BslI CCNNNNNNNGG 1 cut(s) 937
BsmFI GGGAC 1 cut(s) 2
BsnI GGCC 2 cut(s) 490, 667
BsoBI CYCGRG 1 cut(s) 298
Bsp1286I GDGCHC 1 cut(s) 82
Bsp143I GATC 1 cut(s) 592
Bsp1720I GCTNAGC 1 cut(s) 121
BspANI GGCC 2 cut(s) 490, 667
BspCNI CTCAG 4 cut(s) 134, 249, 520, 939
BspFNI CGCG 1 cut(s) 276
BspLI GGNNCC 2 cut(s) 533, 883
BspMAI CTGCAG 2 cut(s) 499, 680
BspPI GGATC 1 cut(s) 587
BsrDI GCAATG 1 cut(s) 429
BsrI ACTGG 2 cut(s) 586, 748
BssMI GATC 1 cut(s) 592
Bst4CI ACNGT 4 cut(s) 486, 731, 822, 868
BstAPI GCANNNNNTGC 2 cut(s) 236, 503
BstC8I GCNNGC 3 cut(s) 445, 669, 673
BstDEI CTNAG 6 cut(s) 121, 257, 321, 435, 507, 926
BstFNI CGCG 1 cut(s) 276
BstKTI GATC 1 cut(s) 595
BstMBI GATC 1 cut(s) 592
BstNSI RCATGY 3 cut(s) 169, 214, 230
BstSFI CTRYAG 2 cut(s) 495, 676
BstUI CGCG 1 cut(s) 276
BstV1I GCAGC 5 cut(s) 55, 137, 406, 490, 662
BsuI GTATCC 2 cut(s) 155, 461
BsuRI GGCC 2 cut(s) 490, 667
BtgZI GCGATG 1 cut(s) 437
BtsIMutI CAGTG 3 cut(s) 254, 482, 864
Cac8I GCNNGC 3 cut(s) 445, 669, 673
CaiI CAGNNNCTG 2 cut(s) 503, 797
Cfr13I GGNCC 1 cut(s) 665
Csp6I GTAC 1 cut(s) 773
CspCI CAANNNNNGTGG 2 cut(s) 835, 870
CviAII CATG 6 cut(s) 166, 211, 227, 283, 399, 942
CviQI GTAC 1 cut(s) 773
DdeI CTNAG 6 cut(s) 121, 257, 321, 435, 507, 926
DpnI GATC 1 cut(s) 594
DpnII GATC 1 cut(s) 592
Ecl136II GAGCTC 1 cut(s) 80
Eco24I GRGCYC 1 cut(s) 82
Eco53kI GAGCTC 1 cut(s) 80
Eco57I CTGAAG 4 cut(s) 203, 662, 681, 774
Eco88I CYCGRG 1 cut(s) 298
EcoICRI GAGCTC 1 cut(s) 80
EcoRI GAATTC 2 cut(s) 278, 695
EcoT38I GRGCYC 1 cut(s) 82
FaeI CATG 6 cut(s) 169, 214, 230, 286, 402, 945
FaqI GGGAC 1 cut(s) 2
FatI CATG 6 cut(s) 165, 210, 226, 282, 398, 941
Fnu4HI GCNGC 5 cut(s) 69, 126, 420, 504, 676
FriOI GRGCYC 1 cut(s) 82
Fsp4HI GCNGC 5 cut(s) 69, 126, 420, 504, 676
FspBI CTAG 1 cut(s) 536
GluI GCNGC 5 cut(s) 69, 126, 420, 504, 676
GsuI CTGGAG 3 cut(s) 51, 603, 765
HaeIII GGCC 2 cut(s) 490, 667
HapII CCGG 1 cut(s) 207
Hin1II CATG 6 cut(s) 169, 214, 230, 286, 402, 945
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HindIII AAGCTT 2 cut(s) 623, 644
HinfI GANTC 3 cut(s) 102, 717, 755
HpaII CCGG 1 cut(s) 207
HphI GGTGA 3 cut(s) 599, 623, 764
Hpy166II GTNNAC 2 cut(s) 199, 340
Hpy188I TCNGA 4 cut(s) 183, 510, 642, 793
Hpy188III TCNNGA 4 cut(s) 61, 108, 616, 808
Hpy8I GTNNAC 2 cut(s) 199, 340
HpyAV CCTTC 7 cut(s) 47, 178, 433, 501, 505, 656, 820
HpyCH4III ACNGT 4 cut(s) 486, 731, 822, 868
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 8 cut(s) 50, 95, 149, 230, 239, 497, 678, 713
HpyF3I CTNAG 6 cut(s) 121, 257, 321, 435, 507, 926
HpySE526I ACGT 1 cut(s) 336
Hsp92II CATG 6 cut(s) 169, 214, 230, 286, 402, 945
Kzo9I GATC 1 cut(s) 592
LmnI GCTCC 4 cut(s) 77, 85, 746, 881
Lsp1109I GCAGC 5 cut(s) 55, 137, 406, 490, 662
LweI GCATC 1 cut(s) 37
MaeI CTAG 1 cut(s) 536
MaeII ACGT 1 cut(s) 336
MaeIII GTNAC 3 cut(s) 250, 655, 752
MalI GATC 1 cut(s) 594
MboI GATC 1 cut(s) 592
MboII GAAGA 1 cut(s) 338
MhlI GDGCHC 1 cut(s) 82
MluCI AATT 6 cut(s) 202, 278, 387, 567, 695, 839
MlyI GAGTC 1 cut(s) 749
MmeI TCCRAC 1 cut(s) 79
MnlI CCTC 7 cut(s) 110, 464, 493, 553, 556, 578, 823
MseI TTAA 2 cut(s) 333, 549
MslI CAYNNNNRTG 1 cut(s) 940
MspI CCGG 1 cut(s) 207
MvnI CGCG 1 cut(s) 276
NdeII GATC 1 cut(s) 592
NlaIII CATG 6 cut(s) 169, 214, 230, 286, 402, 945
NlaIV GGNNCC 2 cut(s) 533, 883
NmuCI GTSAC 1 cut(s) 752
NspI RCATGY 3 cut(s) 169, 214, 230
PfeI GAWTC 2 cut(s) 102, 717
PkrI GCNGC 5 cut(s) 70, 127, 421, 505, 677
PleI GAGTC 1 cut(s) 749
PpsI GAGTC 1 cut(s) 749
Psp124BI GAGCTC 1 cut(s) 82
PspN4I GGNNCC 2 cut(s) 533, 883
PspPI GGNCC 1 cut(s) 665
PstI CTGCAG 2 cut(s) 499, 680
PstNI CAGNNNCTG 2 cut(s) 503, 797
RsaI GTAC 1 cut(s) 774
RsaNI GTAC 1 cut(s) 773
RseI CAYNNNNRTG 1 cut(s) 940
SacI GAGCTC 1 cut(s) 82
SaqAI TTAA 2 cut(s) 333, 549
SatI GCNGC 5 cut(s) 69, 126, 420, 504, 676
Sau3AI GATC 1 cut(s) 592
Sau96I GGNCC 1 cut(s) 665
SchI GAGTC 1 cut(s) 749
SduI GDGCHC 1 cut(s) 82
SfaNI GCATC 1 cut(s) 37
SfcI CTRYAG 2 cut(s) 495, 676
SmiMI CAYNNNNRTG 1 cut(s) 940
SmlI CTYRAG 2 cut(s) 106, 472
SmoI CTYRAG 2 cut(s) 106, 472
Sse9I AATT 6 cut(s) 202, 278, 387, 567, 695, 839
SspI AATATT 1 cut(s) 178
SspMI CTAG 1 cut(s) 536
SstI GAGCTC 1 cut(s) 82
TaaI ACNGT 4 cut(s) 486, 731, 822, 868
TaiI ACGT 1 cut(s) 339
TaqI TCGA 1 cut(s) 377
TasI AATT 6 cut(s) 202, 278, 387, 567, 695, 839
TatI WGTACW 1 cut(s) 772
TfiI GAWTC 2 cut(s) 102, 717
Tru1I TTAA 2 cut(s) 333, 549
Tru9I TTAA 2 cut(s) 333, 549
TscAI CASTG 3 cut(s) 261, 489, 871
TseFI GTSAC 1 cut(s) 752
TseI GCWGC 5 cut(s) 68, 125, 419, 503, 675
Tsp45I GTSAC 1 cut(s) 752
TspDTI ATGAA 3 cut(s) 271, 408, 828
TspRI CASTG 3 cut(s) 261, 489, 871
XapI RAATTY 3 cut(s) 278, 695, 839
XceI RCATGY 3 cut(s) 169, 214, 230
XspI CTAG 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.