Rorug01G0220600

pfkB family carbohydrate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0003570
Physical Location & Seq
Forward (+)
31612908 .. 31616781
3874 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0220600.1

Sequence Viewer

Length: 840 bp
ATGGAGTATTGTGGTGGAGATGTTCTCAGCTCCGCCCCTGATATATTAGAAGCAGAAGTATGGGAAGATGGAGATTGTGGTGGAGCAGAATTCGAGGATAATGTTGGACGACAGACTCCACCCGATGACCTGGATATTGATGGTTGGCTAGATATCAATGACCTAGCCGACATCTATGCAATTGGTTGTCCTTCTTCATCAGCTCGGCCCGGTCTTATAGGTTCCAAGCGGTCGACTCTCTTTCACTCTTTCTCAGGCAAAGCTTGCATTTCTGAAAGGTTACACCTTTTCCACGCGTTGTCGTCATGGATCTGGAGCTTGGGACCATGGACAGCTTCAGATCTAGTTTCTACGTCTGGTGCTAGAAACAATTTTACGAAAGGTCACTACACCGAGAGGGCTGACTTGATAGATTCGGTTCTCAATGCGGTGCGCAAAGAGTCGGAGAACTGTGAGTGCTTGCAAGCAAACTCTATGTTTCAACCTCAGATGGAAAAGGGTGATGGTGCTAGTAATTCTTTGGGGGCTTTTCTACTTCTTCAAATATTCAAGACTGTTCATGAAGGGCGCATATATCAACTAAAACTGTTCTATGACAAAGATTATCTAGAGAAGCCACCAAGTGTACAATTTCATACACGCATCAAGATGACTTGTGTCAATCATGAAACTGGAGTGGTTAAGCATGATGAACTTTTTACCCCTCTTTTTACTGACTTATTACTAGCATATGGGAAACTTTGGATGCTATATGTAACCCAGTTTACGAATATTGTGAAGCAATGTGAATATTCAAATGTTAAAGCTACTGAACTGATTCTCCCACAGTTTGTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00500 ko00520 ko01100 map00051 map00500 map00520 map01100
Pfam Domains
Protein Families
PfkB

Protein Analysis

279

Amino Acids

31.27

Weight (kDa)

4.87

Isoelectric Point (pI)

36.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 434
AccI GTMKAC 1 cut(s) 233
AccII CGCG 1 cut(s) 296
AciI CCGC 3 cut(s) 33, 229, 428
AclWI GGATC 1 cut(s) 317
AcsI RAATTY 1 cut(s) 89
AcuI CTGAAG 1 cut(s) 321
AdeI CACNNNGTG 1 cut(s) 623
AfaI GTAC 1 cut(s) 627
AflIII ACRYGT 1 cut(s) 294
AgsI TTSAA 4 cut(s) 482, 542, 550, 795
AjnI CCWGG 1 cut(s) 129
AloI GAACNNNNNNTCC 2 cut(s) 804, 836
AluBI AGCT 6 cut(s) 30, 203, 263, 318, 335, 806
AluI AGCT 6 cut(s) 30, 203, 263, 318, 335, 806
AlwI GGATC 1 cut(s) 317
AoxI GGCC 1 cut(s) 206
ApoI RAATTY 1 cut(s) 89
Asp700I GAANNNNTTC 1 cut(s) 816
AspLEI GCGC 2 cut(s) 435, 570
AspS9I GGNCC 2 cut(s) 207, 323
AsuC2I CCSGG 1 cut(s) 210
AsuHPI GGTGA 1 cut(s) 512
AvaII GGWCC 1 cut(s) 323
BccI CCATC 4 cut(s) 62, 134, 484, 497
BcgI CGANNNNNNTGC 2 cut(s) 158, 192
BciT130I CCWGG 1 cut(s) 131
BcnI CCSGG 1 cut(s) 210
BfaI CTAG 7 cut(s) 149, 164, 344, 363, 510, 608, 725
BglII AGATCT 1 cut(s) 340
Bme1390I CCNGG 2 cut(s) 131, 210
Bme18I GGWCC 1 cut(s) 323
BmgT120I GGNCC 2 cut(s) 207, 323
BmiI GGNNCC 2 cut(s) 223, 324
BmrFI CCNGG 2 cut(s) 131, 210
BmrI ACTGGG 1 cut(s) 754
BmsI GCATC 2 cut(s) 651, 735
BmuI ACTGGG 1 cut(s) 754
BoxI GACNNNNGTC 1 cut(s) 656
BplI GAGNNNNNCTC 2 cut(s) 9, 41
BpmI CTGGAG 2 cut(s) 334, 693
BpuMI CCSGG 1 cut(s) 210
BsaJI CCNNGG 1 cut(s) 326
BsaXI ACNNNNNCTCC 2 cut(s) 804, 834
Bse1I ACTGG 2 cut(s) 676, 760
Bse3DI GCAATG 1 cut(s) 788
BseBI CCWGG 1 cut(s) 131
BseDI CCNNGG 1 cut(s) 326
BseGI GGATG 1 cut(s) 750
BseMI GCAATG 1 cut(s) 788
BseMII CTCAG 3 cut(s) 40, 267, 500
BseNI ACTGG 2 cut(s) 676, 760
Bsh1236I CGCG 1 cut(s) 296
Bsh1285I CGRYCG 1 cut(s) 233
BshFI GGCC 1 cut(s) 208
BsiEI CGRYCG 1 cut(s) 233
BsiSI CCGG 1 cut(s) 210
BslFI GGGAC 1 cut(s) 336
BsmFI GGGAC 1 cut(s) 336
BsnI GGCC 1 cut(s) 208
Bsp1407I TGTACA 1 cut(s) 625
Bsp143I GATC 2 cut(s) 309, 340
Bsp19I CCATGG 1 cut(s) 326
BspACI CCGC 3 cut(s) 33, 229, 428
BspANI GGCC 1 cut(s) 208
BspCNI CTCAG 3 cut(s) 39, 266, 499
BspFNI CGCG 1 cut(s) 296
BspHI TCATGA 2 cut(s) 559, 664
BspLI GGNNCC 2 cut(s) 223, 324
BspPI GGATC 1 cut(s) 317
BsrDI GCAATG 1 cut(s) 788
BsrGI TGTACA 1 cut(s) 625
BsrI ACTGG 2 cut(s) 676, 760
BssECI CCNNGG 1 cut(s) 326
BssMI GATC 2 cut(s) 309, 340
BssT1I CCWWGG 1 cut(s) 326
Bst2UI CCWGG 1 cut(s) 131
Bst4CI ACNGT 4 cut(s) 452, 556, 588, 828
BstAPI GCANNNNNTGC 1 cut(s) 264
BstAUI TGTACA 1 cut(s) 625
BstC8I GCNNGC 3 cut(s) 265, 461, 465
BstDEI CTNAG 3 cut(s) 26, 253, 486
BstDSI CCRYGG 1 cut(s) 326
BstF5I GGATG 1 cut(s) 750
BstFNI CGCG 1 cut(s) 296
BstHHI GCGC 2 cut(s) 435, 570
BstKTI GATC 2 cut(s) 312, 343
BstMBI GATC 2 cut(s) 309, 340
BstMCI CGRYCG 1 cut(s) 233
BstMWI GCNNNNNNNGC 1 cut(s) 264
BstNI CCWGG 1 cut(s) 131
BstPAI GACNNNNGTC 1 cut(s) 656
BstSCI CCNGG 2 cut(s) 129, 208
BstUI CGCG 1 cut(s) 296
BstX2I RGATCY 2 cut(s) 309, 340
BstYI RGATCY 2 cut(s) 309, 340
BsuRI GGCC 1 cut(s) 208
BtgI CCRYGG 1 cut(s) 326
BtsCI GGATG 1 cut(s) 750
Cac8I GCNNGC 3 cut(s) 265, 461, 465
CciI TCATGA 2 cut(s) 559, 664
CfoI GCGC 2 cut(s) 435, 570
Cfr13I GGNCC 2 cut(s) 207, 323
Csp6I GTAC 1 cut(s) 626
CviAII CATG 5 cut(s) 306, 327, 560, 665, 686
CviQI GTAC 1 cut(s) 626
DdeI CTNAG 3 cut(s) 26, 253, 486
DpnI GATC 2 cut(s) 311, 342
DpnII GATC 2 cut(s) 309, 340
DraIII CACNNNGTG 1 cut(s) 623
EciI GGCGGA 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 326
Eco32I GATATC 1 cut(s) 154
Eco47I GGWCC 1 cut(s) 323
Eco57I CTGAAG 1 cut(s) 321
EcoRI GAATTC 1 cut(s) 89
EcoRII CCWGG 1 cut(s) 129
EcoRV GATATC 1 cut(s) 154
EcoT14I CCWWGG 1 cut(s) 326
ErhI CCWWGG 1 cut(s) 326
FaeI CATG 5 cut(s) 309, 330, 563, 668, 689
FaqI GGGAC 1 cut(s) 336
FatI CATG 5 cut(s) 305, 326, 559, 664, 685
FauNDI CATATG 1 cut(s) 730
FblI GTMKAC 1 cut(s) 233
FokI GGATG 1 cut(s) 757
FspBI CTAG 7 cut(s) 149, 164, 344, 363, 510, 608, 725
FspI TGCGCA 1 cut(s) 434
GlaI GCGC 2 cut(s) 434, 569
GsuI CTGGAG 2 cut(s) 334, 693
HaeIII GGCC 1 cut(s) 208
HapII CCGG 1 cut(s) 210
HhaI GCGC 2 cut(s) 435, 570
Hin1II CATG 5 cut(s) 309, 330, 563, 668, 689
Hin6I GCGC 2 cut(s) 433, 568
HinP1I GCGC 2 cut(s) 433, 568
HincII GTYRAC 1 cut(s) 234
HindII GTYRAC 1 cut(s) 234
HindIII AAGCTT 1 cut(s) 261
HinfI GANTC 5 cut(s) 115, 235, 413, 440, 817
HpaII CCGG 1 cut(s) 210
HphI GGTGA 1 cut(s) 512
Hpy166II GTNNAC 3 cut(s) 234, 626, 765
Hpy188I TCNGA 4 cut(s) 274, 340, 445, 489
Hpy188III TCNNGA 6 cut(s) 313, 550, 560, 608, 646, 665
Hpy8I GTNNAC 3 cut(s) 234, 626, 765
HpyAV CCTTC 2 cut(s) 201, 557
HpyCH4III ACNGT 4 cut(s) 452, 556, 588, 828
HpyCH4IV ACGT 1 cut(s) 353
HpyCH4V TGCA 3 cut(s) 179, 267, 463
HpyF10VI GCNNNNNNNGC 1 cut(s) 264
HpyF3I CTNAG 3 cut(s) 26, 253, 486
HpySE526I ACGT 1 cut(s) 353
Hsp92II CATG 5 cut(s) 309, 330, 563, 668, 689
HspAI GCGC 2 cut(s) 433, 568
Kzo9I GATC 2 cut(s) 309, 340
LmnI GCTCC 3 cut(s) 35, 83, 315
LpnPI CCDG 9 cut(s) 51, 116, 143, 223, 240, 298, 342, 657, 773
LweI GCATC 2 cut(s) 651, 735
MaeI CTAG 7 cut(s) 149, 164, 344, 363, 510, 608, 725
MaeII ACGT 1 cut(s) 353
MaeIII GTNAC 3 cut(s) 279, 383, 754
MalI GATC 2 cut(s) 311, 342
MboI GATC 2 cut(s) 309, 340
MboII GAAGA 3 cut(s) 77, 186, 530
MfeI CAATTG 1 cut(s) 180
MflI RGATCY 2 cut(s) 309, 340
MluCI AATT 5 cut(s) 89, 180, 370, 514, 629
MluI ACGCGT 1 cut(s) 294
MlyI GAGTC 3 cut(s) 109, 229, 449
MmeI TCCRAC 2 cut(s) 85, 423
MnlI CCTC 4 cut(s) 88, 390, 495, 714
MroXI GAANNNNTTC 1 cut(s) 816
MseI TTAA 2 cut(s) 681, 801
MslI CAYNNNNRTG 1 cut(s) 647
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 2 cut(s) 131, 210
MunI CAATTG 1 cut(s) 180
MvaI CCWGG 1 cut(s) 131
MvnI CGCG 1 cut(s) 296
MwoI GCNNNNNNNGC 1 cut(s) 264
NciI CCSGG 1 cut(s) 210
NcoI CCATGG 1 cut(s) 326
NdeI CATATG 1 cut(s) 730
NdeII GATC 2 cut(s) 309, 340
NlaIII CATG 5 cut(s) 309, 330, 563, 668, 689
NlaIV GGNNCC 2 cut(s) 223, 324
NmeAIII GCCGAG 1 cut(s) 184
NmuCI GTSAC 1 cut(s) 383
NsbI TGCGCA 1 cut(s) 434
PagI TCATGA 2 cut(s) 559, 664
PdmI GAANNNNTTC 1 cut(s) 816
PfeI GAWTC 2 cut(s) 413, 817
PleI GAGTC 3 cut(s) 109, 229, 448
PpsI GAGTC 3 cut(s) 109, 229, 448
PshAI GACNNNNGTC 1 cut(s) 656
Psp6I CCWGG 1 cut(s) 129
PspGI CCWGG 1 cut(s) 129
PspN4I GGNNCC 2 cut(s) 223, 324
PspPI GGNCC 2 cut(s) 207, 323
PsuI RGATCY 2 cut(s) 309, 340
RsaI GTAC 1 cut(s) 627
RsaNI GTAC 1 cut(s) 626
RseI CAYNNNNRTG 1 cut(s) 647
SalI GTCGAC 1 cut(s) 232
SaqAI TTAA 2 cut(s) 681, 801
Sau3AI GATC 2 cut(s) 309, 340
Sau96I GGNCC 2 cut(s) 207, 323
SchI GAGTC 3 cut(s) 109, 229, 449
ScrFI CCNGG 2 cut(s) 131, 210
SfaNI GCATC 2 cut(s) 651, 735
SinI GGWCC 1 cut(s) 323
SmiMI CAYNNNNRTG 1 cut(s) 647
Sse9I AATT 5 cut(s) 89, 180, 370, 514, 629
SsiI CCGC 3 cut(s) 33, 229, 428
SspI AATATT 3 cut(s) 546, 772, 791
SspMI CTAG 7 cut(s) 149, 164, 344, 363, 510, 608, 725
StyD4I CCNGG 2 cut(s) 129, 208
StyI CCWWGG 1 cut(s) 326
TaaI ACNGT 4 cut(s) 452, 556, 588, 828
TaiI ACGT 1 cut(s) 356
TaqI TCGA 2 cut(s) 93, 233
TasI AATT 5 cut(s) 89, 180, 370, 514, 629
TatI WGTACW 1 cut(s) 625
TfiI GAWTC 2 cut(s) 413, 817
Tru1I TTAA 2 cut(s) 681, 801
Tru9I TTAA 2 cut(s) 681, 801
TseFI GTSAC 1 cut(s) 383
Tsp45I GTSAC 1 cut(s) 383
TspDTI ATGAA 6 cut(s) 186, 548, 576, 623, 681, 705
VpaK11BI GGWCC 1 cut(s) 323
XapI RAATTY 1 cut(s) 89
XbaI TCTAGA 1 cut(s) 607
XmiI GTMKAC 1 cut(s) 233
XmnI GAANNNNTTC 1 cut(s) 816
XspI CTAG 7 cut(s) 149, 164, 344, 363, 510, 608, 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.