MD02G1197500.v1.1

pfkB family carbohydrate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003570
Physical Location & Seq
Reverse (-)
19106537 .. 19109649
3113 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1197500.v1.1.491

Sequence Viewer

Length: 1119 bp
ATGGCTCCAACAGGATGCAGCTGCTTCCCTGTCACACTGGAGCGATCCAAAAAGGGCAGCTTCAAGTTTGTTAGGAATTCTTCCTCTCTTTCTGGTTCATCTAAATCGTTATCTTTAAAGAAAAAGAGTAGTATCCAAGAGAAGAACTCAGAGCTGATTGTTTGCTTTGGGGAGATGTTGATCGACTTCGTCCCGACTAGTGCCGGAGTTTCCCTTGCAGATGCAGATGGTTTCAAGAAAGCTGCTGGTGGAGCTCCAGCCAATGTTGCAGTTGGAATTTCAAGATTGGGAGGCTCAGCAGCTTTTATGGGCAAGGTTGGTAAAGATGAATTTGGATACATGTTGGCTGACATTCTGAAAGAAAACAATGTGGACAATTCCGGGATGCGTTTTGATCCGCATGCGCGGACTGCATTGGCATTCGTTACACTAAGAAGTGACGGGGAGCGAGAATTCATGTTCTACCGCAACCCAAGTGCTGATATGCTTCTTCAGGAAAAAGAACTTGATGTAAATCTAATTAAAAAGGCCGGCATATTTCACTACGGATCAATTAGTTTGATTGAGGAACCGTGCAGATCAGCTCATCTTGCGGCAATGGACATTGCCAAAAGGGCCGGTTGTATACTCTCTTATGACCCGAATCTGAGATTGCCACTGTGGCCATCTGCAGAGGCTGCTCAGAAAGGCATTATGAGCATATGGAACCAAGCTGAAATTATTAAGATAAGCGAGGAAGAAATTGAATTCTTAACCGGAGGTGATGATCCTTATGATGATGAGGTGGTCTTGAAAAAGCTTTACCACCCCAATCTGAAGCTTCTGCTTGTAACTGAAGGGCCAGCAGGCTGCAGATACTACACAAAGGAATTTAAGGGTAAGGTTGCAGGTATCAAAACTGATGCTGTTGATACTACCGGTGCCGGTGATTCTTTTGTTGCTGCCATACTCAACAGTTTGGGTTCAGACATTAATCTGTACAAGGATGAACAAAAACTTAGAGAAGCTCTCTTGTTTGCCAATGGTTGTGGTGCCCTGACAGTGCAAAAGAAAGGAGCTATTCCTGCACTGCCTACAAAAGAAGCTGTGAACCAACTTCTTCAACAAACCACTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00500 ko00520 ko01100 map00051 map00500 map00520 map01100
Pfam Domains
Protein Families
PfkB

Protein Analysis

373

Amino Acids

40.18

Weight (kDa)

6.19

Isoelectric Point (pI)

33.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 51 - 359 7.6e-76 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 878
AccB1I GGYRCC 2 cut(s) 920, 1031
AccI GTMKAC 1 cut(s) 625
AccII CGCG 1 cut(s) 406
AciI CCGC 4 cut(s) 398, 406, 466, 593
AclWI GGATC 4 cut(s) 39, 389, 556, 761
AcoI YGGCCR 1 cut(s) 662
AcsI RAATTY 6 cut(s) 76, 276, 329, 452, 746, 869
AcuI CTGAAG 3 cut(s) 476, 836, 855
AfaI GTAC 1 cut(s) 980
AflIII ACRYGT 1 cut(s) 339
AgeI ACCGGT 1 cut(s) 917
AgsI TTSAA 6 cut(s) 64, 235, 282, 746, 793, 1103
AhlI ACTAGT 1 cut(s) 197
Alw21I GWGCWC 1 cut(s) 256
AlwI GGATC 4 cut(s) 39, 389, 556, 761
AlwNI CAGNNNCTG 1 cut(s) 677
AoxI GGCC 4 cut(s) 528, 615, 662, 839
ApeKI GCWGC 8 cut(s) 18, 21, 57, 242, 299, 677, 849, 941
ApoI RAATTY 6 cut(s) 76, 276, 329, 452, 746, 869
AseI ATTAAT 1 cut(s) 972
AsiGI ACCGGT 1 cut(s) 917
AspLEI GCGC 1 cut(s) 406
AspS9I GGNCC 2 cut(s) 615, 839
AsuC2I CCSGG 1 cut(s) 382
AsuHPI GGTGA 2 cut(s) 773, 938
BaeGI GKGCMC 1 cut(s) 1036
BalI TGGCCA 1 cut(s) 664
BanI GGYRCC 2 cut(s) 920, 1031
BanII GRGCYC 1 cut(s) 256
Bbv12I GWGCWC 1 cut(s) 256
BbvI GCAGC 8 cut(s) 8, 30, 69, 229, 311, 664, 836, 928
BccI CCATC 2 cut(s) 221, 673
BciVI GTATCC 2 cut(s) 143, 329
BcnI CCSGG 1 cut(s) 382
BcuI ACTAGT 1 cut(s) 197
BfaI CTAG 1 cut(s) 198
BfmI CTRYAG 2 cut(s) 669, 850
BfuAI ACCTGC 1 cut(s) 878
BfuI GTATCC 2 cut(s) 143, 329
BglI GCCNNNNNGGC 2 cut(s) 614, 661
BisI GCNGC 9 cut(s) 19, 22, 58, 243, 300, 594, 678, 850, 942
BlpI GCTNAGC 1 cut(s) 295
BlsI GCNGC 9 cut(s) 20, 23, 59, 244, 301, 595, 679, 851, 943
Bme1390I CCNGG 1 cut(s) 382
BmgT120I GGNCC 2 cut(s) 615, 839
BmiI GGNNCC 5 cut(s) 6, 570, 707, 922, 1033
BmrFI CCNGG 1 cut(s) 382
BmsI GCATC 4 cut(s) 5, 211, 375, 892
BplI GAGNNNNNCTC 4 cut(s) 131, 163, 993, 1025
BpmI CTGGAG 2 cut(s) 59, 240
Bpu1102I GCTNAGC 1 cut(s) 295
BpuMI CCSGG 1 cut(s) 382
BsaBI GATNNNNATC 2 cut(s) 179, 513
BsaWI WCCGGW 2 cut(s) 755, 917
Bse118I RCCGGY 4 cut(s) 530, 617, 917, 923
Bse1I ACTGG 1 cut(s) 42
Bse3DI GCAATG 2 cut(s) 603, 603
Bse8I GATNNNNATC 2 cut(s) 179, 513
BseGI GGATG 3 cut(s) 20, 390, 991
BseJI GATNNNNATC 2 cut(s) 179, 513
BseMI GCAATG 2 cut(s) 603, 603
BseMII CTCAG 4 cut(s) 162, 309, 638, 695
BseNI ACTGG 1 cut(s) 42
BseSI GKGCMC 1 cut(s) 1036
BseXI GCAGC 8 cut(s) 8, 30, 69, 229, 311, 664, 836, 928
BsgI GTGCAG 2 cut(s) 595, 1050
Bsh1236I CGCG 1 cut(s) 406
BshFI GGCC 4 cut(s) 530, 617, 664, 841
BshNI GGYRCC 2 cut(s) 920, 1031
BshTI ACCGGT 1 cut(s) 917
BsiHKAI GWGCWC 1 cut(s) 256
BsiSI CCGG 7 cut(s) 204, 381, 531, 618, 756, 918, 924
BslFI GGGAC 1 cut(s) 176
BsmFI GGGAC 1 cut(s) 176
BsmI GAATGC 1 cut(s) 419
BsnI GGCC 4 cut(s) 530, 617, 664, 841
Bsp1286I GDGCHC 2 cut(s) 256, 1036
Bsp1407I TGTACA 1 cut(s) 978
Bsp143I GATC 6 cut(s) 44, 180, 394, 548, 578, 766
Bsp1720I GCTNAGC 1 cut(s) 295
BspACI CCGC 4 cut(s) 398, 406, 466, 593
BspANI GGCC 4 cut(s) 530, 617, 664, 841
BspCNI CTCAG 4 cut(s) 161, 308, 639, 694
BspFNI CGCG 1 cut(s) 406
BspLI GGNNCC 5 cut(s) 6, 570, 707, 922, 1033
BspMAI CTGCAG 2 cut(s) 673, 854
BspMI ACCTGC 1 cut(s) 878
BspPI GGATC 4 cut(s) 39, 389, 556, 761
BspT107I GGYRCC 2 cut(s) 920, 1031
BsrDI GCAATG 2 cut(s) 603, 603
BsrFI RCCGGY 4 cut(s) 530, 617, 917, 923
BsrGI TGTACA 1 cut(s) 978
BsrI ACTGG 1 cut(s) 42
BssAI RCCGGY 4 cut(s) 530, 617, 917, 923
BssMI GATC 6 cut(s) 44, 180, 394, 548, 578, 766
BssNAI GTATAC 1 cut(s) 626
Bst1107I GTATAC 1 cut(s) 626
Bst4CI ACNGT 4 cut(s) 573, 660, 956, 1042
BstAPI GCANNNNNTGC 1 cut(s) 677
BstAUI TGTACA 1 cut(s) 978
BstC8I GCNNGC 4 cut(s) 402, 532, 843, 847
BstDEI CTNAG 6 cut(s) 148, 295, 431, 647, 681, 998
BstF5I GGATG 3 cut(s) 20, 390, 991
BstFNI CGCG 1 cut(s) 406
BstHHI GCGC 1 cut(s) 406
BstKTI GATC 6 cut(s) 47, 183, 397, 551, 581, 769
BstMBI GATC 6 cut(s) 44, 180, 394, 548, 578, 766
BstMWI GCNNNNNNNGC 9 cut(s) 251, 266, 410, 590, 614, 661, 677, 696, 1064
BstNSI RCATGY 2 cut(s) 343, 404
BstSCI CCNGG 1 cut(s) 380
BstSFI CTRYAG 2 cut(s) 669, 850
BstSLI GKGCMC 1 cut(s) 1036
BstUI CGCG 1 cut(s) 406
BstV1I GCAGC 8 cut(s) 8, 30, 69, 229, 311, 664, 836, 928
BstZ17I GTATAC 1 cut(s) 626
BsuI GTATCC 2 cut(s) 143, 329
BsuRI GGCC 4 cut(s) 530, 617, 664, 841
BtsCI GGATG 3 cut(s) 20, 390, 991
BtsI GCAGTG 1 cut(s) 1067
BtsIMutI CAGTG 5 cut(s) 35, 656, 1047, 1067, 1110
BveI ACCTGC 1 cut(s) 878
Cac8I GCNNGC 4 cut(s) 402, 532, 843, 847
CaiI CAGNNNCTG 1 cut(s) 677
CfoI GCGC 1 cut(s) 406
Cfr10I RCCGGY 4 cut(s) 530, 617, 917, 923
Cfr13I GGNCC 2 cut(s) 615, 839
Csp6I GTAC 1 cut(s) 979
CspAI ACCGGT 1 cut(s) 917
CspCI CAANNNNNGTGG 2 cut(s) 1009, 1044
CviAII CATG 3 cut(s) 340, 401, 457
CviQI GTAC 1 cut(s) 979
DdeI CTNAG 6 cut(s) 148, 295, 431, 647, 681, 998
DpnI GATC 6 cut(s) 46, 182, 396, 550, 580, 768
DpnII GATC 6 cut(s) 44, 180, 394, 548, 578, 766
DraI TTTAAA 1 cut(s) 117
EaeI YGGCCR 1 cut(s) 662
Ecl136II GAGCTC 1 cut(s) 254
Eco24I GRGCYC 1 cut(s) 256
Eco53kI GAGCTC 1 cut(s) 254
Eco57I CTGAAG 3 cut(s) 476, 836, 855
EcoICRI GAGCTC 1 cut(s) 254
EcoRI GAATTC 3 cut(s) 76, 452, 746
EcoT38I GRGCYC 1 cut(s) 256
FaeI CATG 3 cut(s) 343, 404, 460
FalI AAGNNNNNCTT 2 cut(s) 44, 76
FaqI GGGAC 1 cut(s) 176
FatI CATG 3 cut(s) 339, 400, 456
FauNDI CATATG 1 cut(s) 701
FblI GTMKAC 1 cut(s) 625
Fnu4HI GCNGC 9 cut(s) 19, 22, 58, 243, 300, 594, 678, 850, 942
FokI GGATG 3 cut(s) 27, 397, 998
FriOI GRGCYC 1 cut(s) 256
Fsp4HI GCNGC 9 cut(s) 19, 22, 58, 243, 300, 594, 678, 850, 942
FspBI CTAG 1 cut(s) 198
GlaI GCGC 1 cut(s) 405
GluI GCNGC 9 cut(s) 19, 22, 58, 243, 300, 594, 678, 850, 942
GsuI CTGGAG 2 cut(s) 59, 240
HaeIII GGCC 4 cut(s) 530, 617, 664, 841
HapII CCGG 7 cut(s) 204, 381, 531, 618, 756, 918, 924
HhaI GCGC 1 cut(s) 406
Hin1II CATG 3 cut(s) 343, 404, 460
Hin6I GCGC 1 cut(s) 404
HinP1I GCGC 1 cut(s) 404
HindIII AAGCTT 2 cut(s) 797, 818
HinfI GANTC 2 cut(s) 643, 929
HpaII CCGG 7 cut(s) 204, 381, 531, 618, 756, 918, 924
HphI GGTGA 2 cut(s) 773, 938
Hpy166II GTNNAC 3 cut(s) 373, 626, 1090
Hpy188I TCNGA 6 cut(s) 151, 357, 648, 684, 816, 967
Hpy188III TCNNGA 5 cut(s) 193, 235, 282, 494, 790
Hpy8I GTNNAC 3 cut(s) 373, 626, 1090
HpyAV CCTTC 1 cut(s) 830
HpyCH4III ACNGT 4 cut(s) 573, 660, 956, 1042
HpyF10VI GCNNNNNNNGC 9 cut(s) 251, 266, 410, 590, 614, 661, 677, 696, 1064
HpyF3I CTNAG 6 cut(s) 148, 295, 431, 647, 681, 998
Hsp92II CATG 3 cut(s) 343, 404, 460
HspAI GCGC 1 cut(s) 404
KroI GCCGGC 1 cut(s) 530
KroNI GCCGGC 1 cut(s) 532
Kzo9I GATC 6 cut(s) 44, 180, 394, 548, 578, 766
LmnI GCTCC 6 cut(s) 10, 40, 251, 259, 445, 1055
Lsp1109I GCAGC 8 cut(s) 8, 30, 69, 229, 311, 664, 836, 928
LweI GCATC 4 cut(s) 5, 211, 375, 892
MaeI CTAG 1 cut(s) 198
MaeIII GTNAC 4 cut(s) 31, 424, 437, 829
MalI GATC 6 cut(s) 46, 182, 396, 550, 580, 768
MboI GATC 6 cut(s) 44, 180, 394, 548, 578, 766
MboII GAAGA 5 cut(s) 72, 154, 482, 749, 1091
MhlI GDGCHC 2 cut(s) 256, 1036
MlsI TGGCCA 1 cut(s) 664
MluNI TGGCCA 1 cut(s) 664
MmeI TCCRAC 2 cut(s) 32, 253
MnlI CCTC 7 cut(s) 94, 284, 559, 667, 727, 752, 775
Mox20I TGGCCA 1 cut(s) 664
MroNI GCCGGC 1 cut(s) 530
MscI TGGCCA 1 cut(s) 664
MseI TTAA 6 cut(s) 116, 522, 723, 752, 873, 972
Msp20I TGGCCA 1 cut(s) 664
MspA1I CMGCKG 1 cut(s) 21
MspI CCGG 7 cut(s) 204, 381, 531, 618, 756, 918, 924
MspR9I CCNGG 1 cut(s) 382
Mva1269I GAATGC 1 cut(s) 419
MvnI CGCG 1 cut(s) 406
MwoI GCNNNNNNNGC 9 cut(s) 251, 266, 410, 590, 614, 661, 677, 696, 1064
NaeI GCCGGC 1 cut(s) 532
NciI CCSGG 1 cut(s) 382
NdeI CATATG 1 cut(s) 701
NdeII GATC 6 cut(s) 44, 180, 394, 548, 578, 766
NgoMIV GCCGGC 1 cut(s) 530
NlaIII CATG 3 cut(s) 343, 404, 460
NlaIV GGNNCC 5 cut(s) 6, 570, 707, 922, 1033
NmuCI GTSAC 2 cut(s) 31, 437
NspI RCATGY 2 cut(s) 343, 404
PaeI GCATGC 1 cut(s) 404
PciI ACATGT 1 cut(s) 339
PctI GAATGC 1 cut(s) 419
PdiI GCCGGC 1 cut(s) 532
PfeI GAWTC 2 cut(s) 643, 929
PflFI GACNNNGTC 1 cut(s) 188
PfoI TCCNGGA 1 cut(s) 380
PinAI ACCGGT 1 cut(s) 917
PkrI GCNGC 9 cut(s) 20, 23, 59, 244, 301, 595, 679, 851, 943
PscI ACATGT 1 cut(s) 339
PshBI ATTAAT 1 cut(s) 972
Psp124BI GAGCTC 1 cut(s) 256
PspN4I GGNNCC 5 cut(s) 6, 570, 707, 922, 1033
PspPI GGNCC 2 cut(s) 615, 839
PstI CTGCAG 2 cut(s) 673, 854
PstNI CAGNNNCTG 1 cut(s) 677
PsyI GACNNNGTC 1 cut(s) 188
PvuII CAGCTG 1 cut(s) 21
RsaI GTAC 1 cut(s) 980
RsaNI GTAC 1 cut(s) 979
SacI GAGCTC 1 cut(s) 256
SaqAI TTAA 6 cut(s) 116, 522, 723, 752, 873, 972
SatI GCNGC 9 cut(s) 19, 22, 58, 243, 300, 594, 678, 850, 942
Sau3AI GATC 6 cut(s) 44, 180, 394, 548, 578, 766
Sau96I GGNCC 2 cut(s) 615, 839
ScrFI CCNGG 1 cut(s) 382
SduI GDGCHC 2 cut(s) 256, 1036
SfaNI GCATC 4 cut(s) 5, 211, 375, 892
SfcI CTRYAG 2 cut(s) 669, 850
SpeI ACTAGT 1 cut(s) 197
SphI GCATGC 1 cut(s) 404
SsiI CCGC 4 cut(s) 398, 406, 466, 593
SspMI CTAG 1 cut(s) 198
SstI GAGCTC 1 cut(s) 256
StyD4I CCNGG 1 cut(s) 380
TaaI ACNGT 4 cut(s) 573, 660, 956, 1042
TaqI TCGA 1 cut(s) 183
TatI WGTACW 1 cut(s) 978
TauI GCSGC 1 cut(s) 596
TfiI GAWTC 2 cut(s) 643, 929
Tru1I TTAA 6 cut(s) 116, 522, 723, 752, 873, 972
Tru9I TTAA 6 cut(s) 116, 522, 723, 752, 873, 972
TscAI CASTG 5 cut(s) 42, 663, 1047, 1074, 1117
TseFI GTSAC 2 cut(s) 31, 437
TseI GCWGC 8 cut(s) 18, 21, 57, 242, 299, 677, 849, 941
Tsp45I GTSAC 2 cut(s) 31, 437
TspDTI ATGAA 4 cut(s) 87, 342, 445, 1002
TspGWI ACGGA 1 cut(s) 561
TspRI CASTG 5 cut(s) 42, 663, 1047, 1074, 1117
Tth111I GACNNNGTC 1 cut(s) 188
VspI ATTAAT 1 cut(s) 972
XapI RAATTY 6 cut(s) 76, 276, 329, 452, 746, 869
XceI RCATGY 2 cut(s) 343, 404
XmiI GTMKAC 1 cut(s) 625
XspI CTAG 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.