Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0001870

Physical Location & Seq

Genomic Coordinates Reverse (-)

25865868 .. 25878738

Gene Structure 12871 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1120400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1629 bp

ATGCGAGTTTTGATTTGTACGATTGATTTTACTGCAGCCGACGAATCAGAGAAGTTTGTAGAAGCCTCGAAGAAGGGGAATTTAATTCCTCTGTCTCGGAGCATATTCTCGGATCATCTAACTCCTGTTCTCGCTTACCGGTGTCTGGTTAAGGAGGATGATAGAGATGCTCCAAGTTTTCTGTTTGAGTCAGTTGAGCCGGGTTCCAACGACTCCAATGTTGGCCGGCACAGTGTTATTGGTGCCCAACCATCCATTGAAATCGTGGCTAAGGAGAACATGGTTACCATTATGGACCACAAAGAAGGTTGTAGGACAGAGGAGTTTGTGGAGGATCCAATGACTGTTCCTCGGAGAATCATGGAGGGATGGACACCCCAACTTCTTGATGAACTTCCAGAAGCATTTTGTGGCGGCTGGGTTGGTTACTTCTCTTATGATACAGTACGTTATGTTGAGAAGAAAAAGCTGCCATTTCCGAGTGCACCACCAGATGACAGAAATCTTCCTGATGTTCATCTTGGTCTTTATGATGATGTGATAGTGTTCGATCATGTGGACAAGAAAGCACATGTAATTCACTGGGTGCAATTAGATCAATATTCTTCTGTTGACGAGGCCTTCAATGATGGAATAAATAGATTGGAAACATTGCTATCTAGAGTGCATGACATTATCACCCCTAGGCTGCCTGCAGGTTCAATAGAGTTCAACACTCAACTTTTTGGTACTAAATTGGAGGACTCAAGCTTGACAAGTGAGGAATATAAGGAGGCAGTTTTGGAAGCTAAAGAACACATTCTAGCTGGGGATATATTTCAGATTGTATTAAGCCAGCGTTTTGAGAGGCGAACATTTGCAGACCCATTTGAAATTTACCGAGCATTGAGGATAGTTAATCCAAGTCCATATATGACTTATTTACAGGCAAGAGGCTGTATTCTGGTTGCTTCAAGTCCAGAAATTCTGACACGTGTCAAGAATAAAACGATCACCAACCGGCCTCTTGCTGGGACAGTTAGAAGAGGAAAAACACCTAAAGAAGATTTAATGCTGGAAAAGCAGCTTTTGAATGATGAAAAGCAATGTGCAGAGCATATTATGCTTGTAGACTTGGGAAGGAATGATGTTGGGAAGGTCTCAAAACCTGGTTCTGTCAAGGTTGAGGAGCTCATGAACATTGAGCGTTATTCCCATGTCATGCACATAAGTTCAACAGTTACTGGAGAGTTACTTGATGATTTAACTAGCTGGGATGTTCTTCGTACTGCGTTGCCCGTTGGGACAGTCAGTGGAGCACCGAAGGTGAAAGCCATGGAGCTGATCGATAAGCTAGAAGTGACTAGACGTGGTCCATACAGTGGTGGCTTTGGGGGAATTTCGTTTTCTGGCAACATGGACATTGCCCTTGCTCTGAGAACTATTGTTTTTCCAACTAGCTTTCGGTATGATACAATGTATTCATACAAGGACGCAAACAAGCGCAGAGAGTGGGTCGCTCATCTCCAAGCCGGGGCAGGAATTGTTGCTGACAGTGATCCAGCCGATGAGCAGAGGGAGTGCGAGAACAAAGCTGCTGCTCTTGCGCGCGCTATTGATCTGGCGGAGTCCTCGTTCGTTGATAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000162 GO:0003674 GO:0003824 GO:0004049 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005950 GO:0006082 GO:0006520 GO:0006568 GO:0006576 GO:0006586 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009308 GO:0009309 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016829 GO:0016830 GO:0016833 GO:0018130 GO:0019438 GO:0019752 GO:0032991 GO:0034641 GO:0042401 GO:0042430 GO:0042435 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044106 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046219 GO:0046394 GO:0046483 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00400 ko00405 ko01100 ko01110 ko01130 ko01230 ko02024 ko02025 map00400 map00405 map01100 map01110 map01130 map01230 map02024 map02025

Pfam Domains

Protein Families

Anth_synt_I_N Chorismate_bind

Protein Analysis

543

Amino Acids

60.78

Weight (kDa)

5.17

Isoelectric Point (pI)

44.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Anth_synt_I_N	PF04715	38 - 191	2.6e-28	Anthranilate synthase component I, N terminal region
Chorismate_bind	PF00425	253 - 524	2.4e-80	chorismate binding enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001870)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G29690 AT2G29690
fragaria_vesca	FvH4_3g38350 FvH4_3g38350 FvH4_3g38350 FvH4_3g38350 FvH4_3g38350 FvH4_3g38350 FvH4_3g38350
malus_domestica	MD00G1120400.v1.1 MD03G1072500.v1.1 MD11G1077400.v1.1 MD11G1078000.v1.1 MD11G1078700.v1.1
prunus_persica	Prupe.6G059400_v2.0.a1 Prupe.6G059400_v2.0.a1 Prupe.6G059400_v2.0.a1 Prupe.6G059400_v2.0.a1
pyrus_communis	pycom03g05870 pycom11g06530 pycom11g06550 pycom11g06580
rosa_chinensis	RchiOBHm_Chr2g0157091 RchiOBHm_Chr4g0399261 RchiOBHm_Chr5g0068931
rosa_laevigata	RLG00000036024
rosa_multiflora	Rmu_sc0003000.1_g000002 Rmu_sc0006282.1_g000030 Rmu_sc0042660.1_g000001
rosa_roxburghii	Rroxscaffold_1G00011770
rosa_rugosa	Rorug01G0059300 Rorug01G0059400 Rorug07G0214300
rosa_samantha	Rh2BG537500 Rh2DG546300 Rh5AG451600 Rh5BG470700 Rh5CG493100 Rh5DG483500 Rh7BG347700 Rh7BG347800 Rh7DG336200 Rh7DG336300
rosa_wichuraiana	Rw2G031030 Rw2G043250 Rw5G042200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	686
AccB1I	GGYRCC	1 cut(s)	242
AccB7I	CCANNNNNTGG	1 cut(s)	1361
AccI	GTMKAC	1 cut(s)	1110
AccII	CGCG	2 cut(s)	1588, 1590
AciI	CCGC	2 cut(s)	414, 1604
AclWI	GGATC	4 cut(s)	120, 329, 342, 1532
AcoI	YGGCCR	1 cut(s)	223
AcsI	RAATTY	4 cut(s)	79, 873, 963, 1377
AcvI	CACGTG	1 cut(s)	974
AdeI	CACNNNGTG	1 cut(s)	586
AfaI	GTAC	4 cut(s)	19, 447, 730, 1267
AfiI	CCNNNNNNNGG	4 cut(s)	145, 1010, 1361, 1513
AflIII	ACRYGT	3 cut(s)	571, 971, 973
AgeI	ACCGGT	1 cut(s)	138
AgsI	TTSAA	8 cut(s)	260, 625, 702, 712, 872, 954, 1072, 1215
AjiI	CACGTC	1 cut(s)	1349
AjnI	CCWGG	1 cut(s)	1147
AloI	GAACNNNNNNTCC	1 cut(s)	1598
Alw21I	GWGCWC	3 cut(s)	487, 1173, 1300
Alw26I	GTCTC	2 cut(s)	99, 1144
Alw44I	GTGCAC	1 cut(s)	483
AlwI	GGATC	4 cut(s)	120, 329, 342, 1532
AlwNI	CAGNNNCTG	1 cut(s)	1223
AoxI	GGCC	3 cut(s)	223, 618, 1001
ApaLI	GTGCAC	1 cut(s)	483
ApeKI	GCWGC	6 cut(s)	35, 469, 688, 1063, 1574, 1577
ApoI	RAATTY	4 cut(s)	79, 873, 963, 1377
AsiGI	ACCGGT	1 cut(s)	138
Asp700I	GAANNNNTTC	1 cut(s)	798
AspA2I	CCTAGG	1 cut(s)	683
AspLEI	GCGC	4 cut(s)	1485, 1588, 1590, 1592
AspS9I	GGNCC	2 cut(s)	295, 1352
AsuC2I	CCSGG	2 cut(s)	201, 1513
AsuHPI	GGTGA	3 cut(s)	670, 985, 1318
AvaII	GGWCC	2 cut(s)	295, 1352
AvrII	CCTAGG	1 cut(s)	683
BaeGI	GKGCMC	2 cut(s)	247, 487
BamHI	GGATCC	1 cut(s)	334
BanI	GGYRCC	1 cut(s)	242
BanII	GRGCYC	1 cut(s)	1173
BbrPI	CACGTG	1 cut(s)	974
Bbv12I	GWGCWC	3 cut(s)	487, 1173, 1300
BbvI	GCAGC	6 cut(s)	47, 456, 675, 1075, 1561, 1564
BccI	CCATC	3 cut(s)	259, 363, 623
BciT130I	CCWGG	1 cut(s)	1149
BcnI	CCSGG	2 cut(s)	201, 1513
BcoDI	GTCTC	2 cut(s)	99, 1144
BfaI	CTAG	7 cut(s)	660, 684, 803, 1248, 1334, 1344, 1437
BfmI	CTRYAG	2 cut(s)	33, 693
BfuAI	ACCTGC	1 cut(s)	686
BisI	GCNGC	7 cut(s)	36, 415, 470, 689, 1064, 1575, 1578
BlnI	CCTAGG	1 cut(s)	683
BlsI	GCNGC	7 cut(s)	37, 416, 471, 690, 1065, 1576, 1579
Bme1390I	CCNGG	3 cut(s)	201, 1149, 1513
Bme18I	GGWCC	2 cut(s)	295, 1352
BmgBI	CACGTC	1 cut(s)	1349
BmgT120I	GGNCC	2 cut(s)	295, 1352
BmiI	GGNNCC	3 cut(s)	205, 244, 336
BmrFI	CCNGG	3 cut(s)	201, 1149, 1513
BmrI	ACTGGG	1 cut(s)	592
BmsI	GCATC	1 cut(s)	157
BmuI	ACTGGG	1 cut(s)	592
BoxI	GACNNNNGTC	1 cut(s)	974
BpmI	CTGGAG	1 cut(s)	1245
Bpu10I	CCTNAGC	1 cut(s)	270
BpuEI	CTTGAG	1 cut(s)	730
BpuMI	CCSGG	2 cut(s)	201, 1513
Bsa29I	ATCGAT	1 cut(s)	1326
BsaAI	YACGTR	1 cut(s)	974
BsaBI	GATNNNNATC	1 cut(s)	516
BsaI	GGTCTC	1 cut(s)	1144
BsaJI	CCNNGG	4 cut(s)	350, 683, 1314, 1512
BsaWI	WCCGGW	1 cut(s)	138
BsaXI	ACNNNNNCTCC	2 cut(s)	1598, 1628
Bsc4I	CCNNNNNNNGG	4 cut(s)	145, 1010, 1361, 1513
Bse118I	RCCGGY	3 cut(s)	138, 225, 999
Bse1I	ACTGG	2 cut(s)	587, 1228
Bse3DI	GCAATG	3 cut(s)	650, 1091, 1401
Bse8I	GATNNNNATC	1 cut(s)	516
BseBI	CCWGG	1 cut(s)	1149
BseCI	ATCGAT	1 cut(s)	1326
BseDI	CCNNGG	4 cut(s)	350, 683, 1314, 1512
BseGI	GGATG	4 cut(s)	163, 251, 374, 1261
BseJI	GATNNNNATC	1 cut(s)	516
BseLI	CCNNNNNNNGG	4 cut(s)	145, 1010, 1361, 1513
BseMI	GCAATG	3 cut(s)	650, 1091, 1401
BseMII	CTCAG	1 cut(s)	1406
BseNI	ACTGG	2 cut(s)	587, 1228
BsePI	GCGCGC	2 cut(s)	1586, 1588
BseRI	GAGGAG	2 cut(s)	335, 1181
BseSI	GKGCMC	2 cut(s)	247, 487
BseXI	GCAGC	6 cut(s)	47, 456, 675, 1075, 1561, 1564
BseYI	CCCAGC	4 cut(s)	417, 806, 1010, 1251
BsgI	GTGCAG	1 cut(s)	1110
Bsh1236I	CGCG	2 cut(s)	1588, 1590
BshFI	GGCC	3 cut(s)	225, 620, 1003
BshNI	GGYRCC	1 cut(s)	242
BshTI	ACCGGT	1 cut(s)	138
BshVI	ATCGAT	1 cut(s)	1326
BsiHKAI	GWGCWC	3 cut(s)	487, 1173, 1300
BsiSI	CCGG	5 cut(s)	139, 200, 226, 1000, 1512
BslFI	GGGAC	2 cut(s)	1027, 1297
BslI	CCNNNNNNNGG	4 cut(s)	145, 1010, 1361, 1513
BsmAI	GTCTC	2 cut(s)	99, 1144
BsmFI	GGGAC	2 cut(s)	1027, 1297
BsnI	GGCC	3 cut(s)	225, 620, 1003
Bso31I	GGTCTC	1 cut(s)	1144
Bsp1286I	GDGCHC	4 cut(s)	247, 487, 1173, 1300
Bsp143I	GATC	8 cut(s)	112, 334, 550, 595, 990, 1323, 1537, 1597
Bsp19I	CCATGG	1 cut(s)	1314
BspACI	CCGC	2 cut(s)	414, 1604
BspANI	GGCC	3 cut(s)	225, 620, 1003
BspCNI	CTCAG	1 cut(s)	1407
BspDI	ATCGAT	1 cut(s)	1326
BspFNI	CGCG	2 cut(s)	1588, 1590
BspHI	TCATGA	1 cut(s)	1173
BspLI	GGNNCC	3 cut(s)	205, 244, 336
BspMAI	CTGCAG	2 cut(s)	37, 697
BspMI	ACCTGC	1 cut(s)	686
BspPI	GGATC	4 cut(s)	120, 329, 342, 1532
BspT107I	GGYRCC	1 cut(s)	242
BspTNI	GGTCTC	1 cut(s)	1144
BsrDI	GCAATG	3 cut(s)	650, 1091, 1401
BsrFI	RCCGGY	3 cut(s)	138, 225, 999
BsrI	ACTGG	2 cut(s)	587, 1228
BssAI	RCCGGY	3 cut(s)	138, 225, 999
BssECI	CCNNGG	4 cut(s)	350, 683, 1314, 1512
BssHII	GCGCGC	2 cut(s)	1586, 1588
BssMI	GATC	8 cut(s)	112, 334, 550, 595, 990, 1323, 1537, 1597
BssT1I	CCWWGG	2 cut(s)	683, 1314
Bst2UI	CCWGG	1 cut(s)	1149
Bst4CI	ACNGT	8 cut(s)	233, 346, 445, 1018, 1219, 1288, 1361, 1535
Bst6I	CTCTTC	1 cut(s)	1018
BstAPI	GCANNNNNTGC	1 cut(s)	1102
BstBAI	YACGTR	1 cut(s)	974
BstC8I	GCNNGC	5 cut(s)	227, 693, 836, 1588, 1590
BstDEI	CTNAG	2 cut(s)	270, 1415
BstDSI	CCRYGG	1 cut(s)	1314
BstEII	GGTNACC	1 cut(s)	283
BstF5I	GGATG	4 cut(s)	163, 251, 374, 1261
BstFNI	CGCG	2 cut(s)	1588, 1590
BstHHI	GCGC	4 cut(s)	1485, 1588, 1590, 1592
BstKTI	GATC	8 cut(s)	115, 337, 553, 598, 993, 1326, 1540, 1600
BstMAI	GTCTC	2 cut(s)	99, 1144
BstMBI	GATC	8 cut(s)	112, 334, 550, 595, 990, 1323, 1537, 1597
BstMWI	GCNNNNNNNGC	3 cut(s)	1060, 1102, 1583
BstNI	CCWGG	1 cut(s)	1149
BstNSI	RCATGY	1 cut(s)	575
BstPAI	GACNNNNGTC	1 cut(s)	974
BstPI	GGTNACC	1 cut(s)	283
BstSCI	CCNGG	3 cut(s)	199, 1147, 1511
BstSFI	CTRYAG	2 cut(s)	33, 693
BstSLI	GKGCMC	2 cut(s)	247, 487
BstUI	CGCG	2 cut(s)	1588, 1590
BstV1I	GCAGC	6 cut(s)	47, 456, 675, 1075, 1561, 1564
BstX2I	RGATCY	1 cut(s)	334
BstYI	RGATCY	1 cut(s)	334
Bsu15I	ATCGAT	1 cut(s)	1326
BsuRI	GGCC	3 cut(s)	225, 620, 1003
BsuTUI	ATCGAT	1 cut(s)	1326
BtgI	CCRYGG	1 cut(s)	1314
BtrI	CACGTC	1 cut(s)	1349
BtsCI	GGATG	4 cut(s)	163, 251, 374, 1261
BtsIMutI	CAGTG	5 cut(s)	238, 580, 1297, 1366, 1540
BveI	ACCTGC	1 cut(s)	686
Cac8I	GCNNGC	5 cut(s)	227, 693, 836, 1588, 1590
CaiI	CAGNNNCTG	1 cut(s)	1223
CciI	TCATGA	1 cut(s)	1173
CfoI	GCGC	4 cut(s)	1485, 1588, 1590, 1592
Cfr10I	RCCGGY	3 cut(s)	138, 225, 999
Cfr13I	GGNCC	2 cut(s)	295, 1352
ClaI	ATCGAT	1 cut(s)	1326
CseI	GACGC	1 cut(s)	1481
CsiI	ACCWGGT	1 cut(s)	1147
Csp6I	GTAC	4 cut(s)	18, 446, 729, 1266
CspAI	ACCGGT	1 cut(s)	138
CviQI	GTAC	4 cut(s)	18, 446, 729, 1266
DdeI	CTNAG	2 cut(s)	270, 1415
DpnI	GATC	8 cut(s)	114, 336, 552, 597, 992, 1325, 1539, 1599
DpnII	GATC	8 cut(s)	112, 334, 550, 595, 990, 1323, 1537, 1597
DraIII	CACNNNGTG	1 cut(s)	586
EaeI	YGGCCR	1 cut(s)	223
Eam1104I	CTCTTC	1 cut(s)	1018
EarI	CTCTTC	1 cut(s)	1018
EciI	GGCGGA	1 cut(s)	1619
Ecl136II	GAGCTC	1 cut(s)	1171
Eco130I	CCWWGG	2 cut(s)	683, 1314
Eco147I	AGGCCT	1 cut(s)	620
Eco24I	GRGCYC	1 cut(s)	1173
Eco31I	GGTCTC	1 cut(s)	1144
Eco47I	GGWCC	2 cut(s)	295, 1352
Eco53kI	GAGCTC	1 cut(s)	1171
Eco72I	CACGTG	1 cut(s)	974
Eco91I	GGTNACC	1 cut(s)	283
EcoICRI	GAGCTC	1 cut(s)	1171
EcoO65I	GGTNACC	1 cut(s)	283
EcoRII	CCWGG	1 cut(s)	1147
EcoT14I	CCWWGG	2 cut(s)	683, 1314
EcoT38I	GRGCYC	1 cut(s)	1173
ErhI	CCWWGG	2 cut(s)	683, 1314
FaqI	GGGAC	2 cut(s)	1027, 1297
FblI	GTMKAC	1 cut(s)	1110
Fnu4HI	GCNGC	7 cut(s)	36, 415, 470, 689, 1064, 1575, 1578
FokI	GGATG	4 cut(s)	170, 238, 381, 1268
FriOI	GRGCYC	1 cut(s)	1173
Fsp4HI	GCNGC	7 cut(s)	36, 415, 470, 689, 1064, 1575, 1578
FspBI	CTAG	7 cut(s)	660, 684, 803, 1248, 1334, 1344, 1437
GlaI	GCGC	4 cut(s)	1484, 1587, 1589, 1591
GluI	GCNGC	7 cut(s)	36, 415, 470, 689, 1064, 1575, 1578
GsaI	CCCAGC	4 cut(s)	421, 810, 1014, 1255
GsuI	CTGGAG	1 cut(s)	1245
HaeIII	GGCC	3 cut(s)	225, 620, 1003
HapII	CCGG	5 cut(s)	139, 200, 226, 1000, 1512
HgaI	GACGC	1 cut(s)	1481
HhaI	GCGC	4 cut(s)	1485, 1588, 1590, 1592
Hin6I	GCGC	4 cut(s)	1483, 1586, 1588, 1590
HinP1I	GCGC	4 cut(s)	1483, 1586, 1588, 1590
HincII	GTYRAC	1 cut(s)	613
HindII	GTYRAC	1 cut(s)	613
HindIII	AAGCTT	1 cut(s)	748
HinfI	GANTC	6 cut(s)	44, 188, 212, 357, 743, 1607
HpaII	CCGG	5 cut(s)	139, 200, 226, 1000, 1512
HphI	GGTGA	3 cut(s)	670, 985, 1318
Hpy166II	GTNNAC	4 cut(s)	485, 559, 613, 1111
Hpy188I	TCNGA	8 cut(s)	49, 99, 112, 354, 480, 822, 969, 1416
Hpy188III	TCNNGA	7 cut(s)	386, 398, 509, 660, 959, 979, 1174
Hpy8I	GTNNAC	4 cut(s)	485, 559, 613, 1111
Hpy99I	CGWCG	1 cut(s)	44
HpyAV	CCTTC	6 cut(s)	67, 299, 631, 1113, 1129, 1297
HpyCH4III	ACNGT	8 cut(s)	233, 346, 445, 1018, 1219, 1288, 1361, 1535
HpyCH4IV	ACGT	3 cut(s)	448, 973, 1348
HpyCH4V	TGCA	8 cut(s)	35, 485, 589, 667, 695, 860, 1091, 1204
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1060, 1102, 1583
HpyF3I	CTNAG	2 cut(s)	270, 1415
HpySE526I	ACGT	3 cut(s)	448, 973, 1348
HspAI	GCGC	4 cut(s)	1483, 1586, 1588, 1590
KroI	GCCGGC	1 cut(s)	225
KroNI	GCCGGC	1 cut(s)	227
Kzo9I	GATC	8 cut(s)	112, 334, 550, 595, 990, 1323, 1537, 1597
LmnI	GCTCC	5 cut(s)	99, 175, 1168, 1295, 1318
Lsp1109I	GCAGC	6 cut(s)	47, 456, 675, 1075, 1561, 1564
LweI	GCATC	1 cut(s)	157
MabI	ACCWGGT	1 cut(s)	1147
MaeI	CTAG	7 cut(s)	660, 684, 803, 1248, 1334, 1344, 1437
MaeII	ACGT	3 cut(s)	448, 973, 1348
MaeIII	GTNAC	5 cut(s)	283, 425, 1219, 1230, 1339
MalI	GATC	8 cut(s)	114, 336, 552, 597, 992, 1325, 1539, 1599
MboI	GATC	8 cut(s)	112, 334, 550, 595, 990, 1323, 1537, 1597
MboII	GAAGA	7 cut(s)	82, 472, 497, 597, 1035, 1055, 1253
MflI	RGATCY	1 cut(s)	334
MhlI	GDGCHC	4 cut(s)	247, 487, 1173, 1300
MlyI	GAGTC	4 cut(s)	197, 206, 737, 1616
MmeI	TCCRAC	2 cut(s)	231, 1457
MroNI	GCCGGC	1 cut(s)	225
MroXI	GAANNNNTTC	1 cut(s)	798
MseI	TTAA	6 cut(s)	83, 150, 830, 897, 1049, 1244
MspI	CCGG	5 cut(s)	139, 200, 226, 1000, 1512
MspR9I	CCNGG	3 cut(s)	201, 1149, 1513
MvaI	CCWGG	1 cut(s)	1149
MvnI	CGCG	2 cut(s)	1588, 1590
MwoI	GCNNNNNNNGC	3 cut(s)	1060, 1102, 1583
NaeI	GCCGGC	1 cut(s)	227
NciI	CCSGG	2 cut(s)	201, 1513
NcoI	CCATGG	1 cut(s)	1314
NdeII	GATC	8 cut(s)	112, 334, 550, 595, 990, 1323, 1537, 1597
NgoMIV	GCCGGC	1 cut(s)	225
NlaIV	GGNNCC	3 cut(s)	205, 244, 336
NmuCI	GTSAC	1 cut(s)	1339
NspI	RCATGY	1 cut(s)	575
PagI	TCATGA	1 cut(s)	1173
PauI	GCGCGC	2 cut(s)	1586, 1588
PceI	AGGCCT	1 cut(s)	620
PciI	ACATGT	1 cut(s)	571
PdiI	GCCGGC	1 cut(s)	227
PdmI	GAANNNNTTC	1 cut(s)	798
PfeI	GAWTC	2 cut(s)	44, 357
PflFI	GACNNNGTC	1 cut(s)	1350
PflMI	CCANNNNNTGG	1 cut(s)	1361
PinAI	ACCGGT	1 cut(s)	138
PkrI	GCNGC	7 cut(s)	37, 416, 471, 690, 1065, 1576, 1579
PleI	GAGTC	4 cut(s)	196, 206, 737, 1615
PmaCI	CACGTG	1 cut(s)	974
PmlI	CACGTG	1 cut(s)	974
PpsI	GAGTC	4 cut(s)	196, 206, 737, 1615
Ppu21I	YACGTR	1 cut(s)	974
PscI	ACATGT	1 cut(s)	571
PshAI	GACNNNNGTC	1 cut(s)	974
Psp124BI	GAGCTC	1 cut(s)	1173
Psp6I	CCWGG	1 cut(s)	1147
PspCI	CACGTG	1 cut(s)	974
PspEI	GGTNACC	1 cut(s)	283
PspFI	CCCAGC	4 cut(s)	417, 806, 1010, 1251
PspGI	CCWGG	1 cut(s)	1147
PspN4I	GGNNCC	3 cut(s)	205, 244, 336
PspPI	GGNCC	2 cut(s)	295, 1352
PstI	CTGCAG	2 cut(s)	37, 697
PstNI	CAGNNNCTG	1 cut(s)	1223
PsuI	RGATCY	1 cut(s)	334
PsyI	GACNNNGTC	1 cut(s)	1350
PteI	GCGCGC	2 cut(s)	1586, 1588
RsaI	GTAC	4 cut(s)	19, 447, 730, 1267
RsaNI	GTAC	4 cut(s)	18, 446, 729, 1266
SacI	GAGCTC	1 cut(s)	1173
SaqAI	TTAA	6 cut(s)	83, 150, 830, 897, 1049, 1244
SatI	GCNGC	7 cut(s)	36, 415, 470, 689, 1064, 1575, 1578
Sau3AI	GATC	8 cut(s)	112, 334, 550, 595, 990, 1323, 1537, 1597
Sau96I	GGNCC	2 cut(s)	295, 1352
SbfI	CCTGCAGG	1 cut(s)	697
SchI	GAGTC	4 cut(s)	197, 206, 737, 1616
ScrFI	CCNGG	3 cut(s)	201, 1149, 1513
SdaI	CCTGCAGG	1 cut(s)	697
SduI	GDGCHC	4 cut(s)	247, 487, 1173, 1300
SexAI	ACCWGGT	1 cut(s)	1147
SfaNI	GCATC	1 cut(s)	157
SfcI	CTRYAG	2 cut(s)	33, 693
SinI	GGWCC	2 cut(s)	295, 1352
SmlI	CTYRAG	1 cut(s)	745
SmoI	CTYRAG	1 cut(s)	745
Sse8387I	CCTGCAGG	1 cut(s)	697
SseBI	AGGCCT	1 cut(s)	620
SsiI	CCGC	2 cut(s)	414, 1604
SspI	AATATT	1 cut(s)	602
SspMI	CTAG	7 cut(s)	660, 684, 803, 1248, 1334, 1344, 1437
SstI	GAGCTC	1 cut(s)	1173
StuI	AGGCCT	1 cut(s)	620
StyD4I	CCNGG	3 cut(s)	199, 1147, 1511
StyI	CCWWGG	2 cut(s)	683, 1314
TaaI	ACNGT	8 cut(s)	233, 346, 445, 1018, 1219, 1288, 1361, 1535
TaiI	ACGT	3 cut(s)	451, 976, 1351
TaqI	TCGA	3 cut(s)	68, 549, 1326
TauI	GCSGC	1 cut(s)	417
TfiI	GAWTC	2 cut(s)	44, 357
Tru1I	TTAA	6 cut(s)	83, 150, 830, 897, 1049, 1244
Tru9I	TTAA	6 cut(s)	83, 150, 830, 897, 1049, 1244
TscAI	CASTG	5 cut(s)	238, 587, 1297, 1366, 1540
TseFI	GTSAC	1 cut(s)	1339
TseI	GCWGC	6 cut(s)	35, 469, 688, 1063, 1574, 1577
Tsp45I	GTSAC	1 cut(s)	1339
TspDTI	ATGAA	5 cut(s)	405, 506, 1092, 1190, 1452
TspRI	CASTG	5 cut(s)	238, 587, 1297, 1366, 1540
Tth111I	GACNNNGTC	1 cut(s)	1350
Van91I	CCANNNNNTGG	1 cut(s)	1361
VneI	GTGCAC	1 cut(s)	483
VpaK11BI	GGWCC	2 cut(s)	295, 1352
XapI	RAATTY	4 cut(s)	79, 873, 963, 1377
XbaI	TCTAGA	1 cut(s)	659
XceI	RCATGY	1 cut(s)	575
XcmI	CCANNNNNNNNNTGG	1 cut(s)	262
XmaJI	CCTAGG	1 cut(s)	683
XmiI	GTMKAC	1 cut(s)	1110
XmnI	GAANNNNTTC	1 cut(s)	798
XspI	CTAG	7 cut(s)	660, 684, 803, 1248, 1334, 1344, 1437

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.