Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0001870

Physical Location & Seq

Genomic Coordinates Reverse (-)

74576691 .. 74581601

Gene Structure 4911 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG537500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1611 bp

ATGCTGTACGGAGTTCTTGTGAAAAATTTGGTTATAGCACTGGAAATATGTTTAAAGTATTCTAATGTGAGGTTTGATTGGAATGCTGCTACAGCTGAGCAGTCAGAGAGGTTTTTCGAGGCTTCGAAAAATGGGAATTTAATTCCTTTATACCGAAGTATATTTTCGGATCACTTAACTCCTGTGCTTGCTTACCGGTGTCTGGTTAAGGAGGATGATAGAGACGCTCCGAGTTTTCTTTTTGAGGCAGTTGATCCGGGTTTCAAGGACTCCAATGTTGGGCGGTACAGTATTATTGGAGCGCAACCAACTATTGAGATCGTAGCAAAAGAGAACATGGTTACAGTTATGGACCATAGAGAAGGGCGTAGGACAGAGGAGATTGTGGAGGATCCAATGACTGTTCCTCAGAGAATCACGGAGGGATGGATGCCGCAACGTCTTGATGAGATGCCAGAAGCATTTTGTGGTGGATGGGTTGGTTACTTCTCTTATGATACAGTGCGTTATGTGGAGAAGAAAAAGCTGCCATTCAGTGGTGCACCACCAGATGACAGAAATCTTCCTGATCTTCATCTTGGCCTGTATGATAGTGTGATAGTGTTTGATCATGTGGAGAAGAAAGCACATGTAATTCACTGGGTGCAATTAGATCGGTATTCTTCAGTTGAGGAGGCCTTCAATGATGGGATGAATAGATTGGAAACACTGGTATCTAGAGTGCATGACATTATCACCCCGAGGCTGCCTGCAGGTTCAATAAAGTTACACACTCATCTATTTGGTGCTAAATTGGAGGATTCAAACATGACAAGTGAGGAATACAAGGAAGCAGTTTTGAAATCTAAAGAACACATCTTAGCTGGGGATATATTTCAGATAGTATTAAGTCAGCGCTTTGAAAGGCGAACATTTGCAGACCCATTCGAAATTTACCGATCAGGCTGTATTCTGGTTGCTTCAAGTCCAGAAATTCTTACACGTGTTAAGAATAGAACAATCACCAATCGACCTCTTGCTGGGACTGTTAGAAGAGGAAAAACACCTAAAGAAGATCTAATGTTGGAAAATCAGCTTTTGAATGATGAAAAGCAATGCGCAGAGCACATTATGCTTGTTGACTTGGGGAGGAATGATGTCGGGAAGGTCTCAAAACCTGGTTCTGTCAAAGTTGAGAAGCTCATGAACATTGAGCGTTATTCCCATGTCATGCACATAAGTTCTACTGTCACTGGAGAGTTGCTTGATCATTTAAGTAGCTGGGATGTTCTACGAACTGCATTGCCGGTTGGGACTGTCAGCGGAGCACCAAAGGTAAAAGCCATGGAGCTGATTGATGAGCTGGAAGTGACTAGACGTGGTCCATACAGTGGCGGTTTTGGAGGAATTTCATTTTCTGGAAACATGGACATCGCCCTTGCTCTGAGAACTATTGTTTTCCCAACTAGCTTTCGATATGATACAATGTATTCATACAAGGACACAAATAAGCGGCGAGAGTGGGTTGCTCATCTCCAAGCCGGAGCTGGAATTGTGGCCGACAGTGTTCCAGCTGAGGAGCAGAGAGAGTGTGAGAACAAAGCTGCTGGCTGGTCTTGCACGGGCTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000162 GO:0003674 GO:0003824 GO:0004049 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005950 GO:0006082 GO:0006520 GO:0006568 GO:0006576 GO:0006586 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009308 GO:0009309 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016829 GO:0016830 GO:0016833 GO:0018130 GO:0019438 GO:0019752 GO:0032991 GO:0034641 GO:0042401 GO:0042430 GO:0042435 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044106 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046219 GO:0046394 GO:0046483 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00400 ko00405 ko01100 ko01110 ko01130 ko01230 ko02024 ko02025 map00400 map00405 map01100 map01110 map01130 map01230 map02024 map02025

Pfam Domains

Protein Families

Anth_synt_I_N Chorismate_bind

Protein Analysis

536

Amino Acids

60.55

Weight (kDa)

5.83

Isoelectric Point (pI)

43.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Anth_synt_I_N	PF04715	57 - 210	3.1e-28	Anthranilate synthase component I, N terminal region
Chorismate_bind	PF00425	272 - 527	6.1e-68	chorismate binding enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001870)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G29690 AT2G29690
fragaria_vesca	FvH4_3g38350 FvH4_3g38350 FvH4_3g38350 FvH4_3g38350 FvH4_3g38350 FvH4_3g38350 FvH4_3g38350
malus_domestica	MD00G1120400.v1.1 MD03G1072500.v1.1 MD11G1077400.v1.1 MD11G1078000.v1.1 MD11G1078700.v1.1
prunus_persica	Prupe.6G059400_v2.0.a1 Prupe.6G059400_v2.0.a1 Prupe.6G059400_v2.0.a1 Prupe.6G059400_v2.0.a1
pyrus_communis	pycom03g05870 pycom11g06530 pycom11g06550 pycom11g06580
rosa_chinensis	RchiOBHm_Chr2g0157091 RchiOBHm_Chr4g0399261 RchiOBHm_Chr5g0068931
rosa_laevigata	RLG00000036024
rosa_multiflora	Rmu_sc0003000.1_g000002 Rmu_sc0006282.1_g000030 Rmu_sc0042660.1_g000001
rosa_roxburghii	Rroxscaffold_1G00011770
rosa_rugosa	Rorug01G0059300 Rorug01G0059400 Rorug07G0214300
rosa_samantha	Rh2BG537500 Rh2DG546300 Rh5AG451600 Rh5BG470700 Rh5CG493100 Rh5DG483500 Rh7BG347700 Rh7BG347800 Rh7DG336200 Rh7DG336300
rosa_wichuraiana	Rw2G031030 Rw2G043250 Rw5G042200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1099
Acc36I	ACCTGC	1 cut(s)	743
AccB7I	CCANNNNNTGG	2 cut(s)	536, 1370
AciI	CCGC	5 cut(s)	283, 434, 1302, 1374, 1492
AclWI	GGATC	4 cut(s)	177, 248, 386, 399
AcoI	YGGCCR	1 cut(s)	1536
AcsI	RAATTY	5 cut(s)	25, 136, 930, 972, 1386
AcuI	CTGAAG	1 cut(s)	648
AcvI	CACGTG	1 cut(s)	983
AdeI	CACNNNGTG	1 cut(s)	643
AfaI	GTAC	2 cut(s)	8, 287
AfeI	AGCGCT	1 cut(s)	896
AfiI	CCNNNNNNNGG	5 cut(s)	202, 279, 536, 1019, 1370
AflIII	ACRYGT	3 cut(s)	628, 980, 982
AgeI	ACCGGT	1 cut(s)	195
AgsI	TTSAA	8 cut(s)	265, 682, 759, 804, 841, 902, 963, 1081
AjiI	CACGTC	1 cut(s)	1358
AjnI	CCWGG	1 cut(s)	1156
Alw21I	GWGCWC	3 cut(s)	544, 1107, 1309
Alw26I	GTCTC	2 cut(s)	216, 1153
Alw44I	GTGCAC	1 cut(s)	540
AlwI	GGATC	4 cut(s)	177, 248, 386, 399
Ama87I	CYCGRG	1 cut(s)	739
Aor51HI	AGCGCT	1 cut(s)	896
AoxI	GGCC	3 cut(s)	580, 675, 1536
ApaLI	GTGCAC	1 cut(s)	540
ApeKI	GCWGC	4 cut(s)	86, 526, 745, 1583
ApoI	RAATTY	5 cut(s)	25, 136, 930, 972, 1386
AsiGI	ACCGGT	1 cut(s)	195
AspLEI	GCGC	3 cut(s)	304, 897, 1100
AspS9I	GGNCC	2 cut(s)	352, 1361
AsuC2I	CCSGG	1 cut(s)	258
AsuHPI	GGTGA	2 cut(s)	727, 994
AsuII	TTCGAA	2 cut(s)	125, 927
AvaI	CYCGRG	1 cut(s)	739
AvaII	GGWCC	2 cut(s)	352, 1361
BaeGI	GKGCMC	1 cut(s)	544
BaeI	ACNNNNGTAYC	2 cut(s)	696, 729
BamHI	GGATCC	1 cut(s)	391
BbrPI	CACGTG	1 cut(s)	983
Bbv12I	GWGCWC	3 cut(s)	544, 1107, 1309
BbvCI	CCTCAGC	1 cut(s)	1554
BbvI	GCAGC	4 cut(s)	73, 513, 732, 1570
BccI	CCATC	3 cut(s)	420, 468, 680
BcgI	CGANNNNNNTGC	2 cut(s)	635, 669
BciT130I	CCWGG	1 cut(s)	1158
BclI	TGATCA	2 cut(s)	607, 1246
BcnI	CCSGG	1 cut(s)	258
BcoDI	GTCTC	2 cut(s)	216, 1153
BfaI	CTAG	3 cut(s)	717, 1353, 1446
BfmI	CTRYAG	2 cut(s)	90, 750
BfoI	RGCGCY	1 cut(s)	898
BfuAI	ACCTGC	1 cut(s)	743
BglII	AGATCT	1 cut(s)	1054
BisI	GCNGC	6 cut(s)	87, 434, 527, 746, 1493, 1584
BlpI	GCTNAGC	1 cut(s)	96
BlsI	GCNGC	6 cut(s)	88, 435, 528, 747, 1494, 1585
Bme1390I	CCNGG	2 cut(s)	258, 1158
Bme18I	GGWCC	2 cut(s)	352, 1361
BmeT110I	CYCGRG	1 cut(s)	739
BmgBI	CACGTC	1 cut(s)	1358
BmgT120I	GGNCC	2 cut(s)	352, 1361
BmiI	GGNNCC	1 cut(s)	393
BmrFI	CCNGG	2 cut(s)	258, 1158
BmrI	ACTGGG	1 cut(s)	649
BmsI	GCATC	2 cut(s)	420, 441
BmuI	ACTGGG	1 cut(s)	649
BpmI	CTGGAG	1 cut(s)	1254
Bpu10I	CCTNAGC	1 cut(s)	1554
Bpu1102I	GCTNAGC	1 cut(s)	96
Bpu14I	TTCGAA	2 cut(s)	125, 927
BpuMI	CCSGG	1 cut(s)	258
BsaAI	YACGTR	1 cut(s)	983
BsaBI	GATNNNNATC	1 cut(s)	573
BsaI	GGTCTC	1 cut(s)	1153
BsaJI	CCNNGG	2 cut(s)	740, 1323
BsaWI	WCCGGW	1 cut(s)	195
BsaXI	ACNNNNNCTCC	1 cut(s)	33
Bsc4I	CCNNNNNNNGG	5 cut(s)	202, 279, 536, 1019, 1370
Bse118I	RCCGGY	2 cut(s)	195, 1285
Bse1I	ACTGG	4 cut(s)	45, 644, 714, 1237
Bse3DI	GCAATG	2 cut(s)	1100, 1280
Bse8I	GATNNNNATC	1 cut(s)	573
BseBI	CCWGG	1 cut(s)	1158
BseDI	CCNNGG	2 cut(s)	740, 1323
BseGI	GGATG	6 cut(s)	220, 431, 435, 479, 696, 1270
BseJI	GATNNNNATC	1 cut(s)	573
BseLI	CCNNNNNNNGG	5 cut(s)	202, 279, 536, 1019, 1370
BseMI	GCAATG	2 cut(s)	1100, 1280
BseMII	CTCAG	4 cut(s)	87, 422, 1415, 1545
BseNI	ACTGG	4 cut(s)	45, 644, 714, 1237
BseRI	GAGGAG	3 cut(s)	392, 686, 1571
BseSI	GKGCMC	1 cut(s)	544
BseXI	GCAGC	4 cut(s)	73, 513, 732, 1570
BseYI	CCCAGC	3 cut(s)	863, 1019, 1260
BshFI	GGCC	3 cut(s)	582, 677, 1538
BshTI	ACCGGT	1 cut(s)	195
BsiHKAI	GWGCWC	3 cut(s)	544, 1107, 1309
BsiHKCI	CYCGRG	1 cut(s)	739
BsiSI	CCGG	4 cut(s)	196, 257, 1286, 1521
BslFI	GGGAC	2 cut(s)	1036, 1306
BslI	CCNNNNNNNGG	5 cut(s)	202, 279, 536, 1019, 1370
BsmAI	GTCTC	2 cut(s)	216, 1153
BsmBI	CGTCTC	1 cut(s)	216
BsmFI	GGGAC	2 cut(s)	1036, 1306
BsmI	GAATGC	1 cut(s)	88
BsnI	GGCC	3 cut(s)	582, 677, 1538
Bso31I	GGTCTC	1 cut(s)	1153
BsoBI	CYCGRG	1 cut(s)	739
Bsp119I	TTCGAA	2 cut(s)	125, 927
Bsp1286I	GDGCHC	3 cut(s)	544, 1107, 1309
Bsp1720I	GCTNAGC	1 cut(s)	96
Bsp19I	CCATGG	1 cut(s)	1323
BspACI	CCGC	5 cut(s)	283, 434, 1302, 1374, 1492
BspANI	GGCC	3 cut(s)	582, 677, 1538
BspCNI	CTCAG	4 cut(s)	88, 421, 1416, 1546
BspHI	TCATGA	1 cut(s)	1182
BspLI	GGNNCC	1 cut(s)	393
BspMAI	CTGCAG	1 cut(s)	754
BspMI	ACCTGC	1 cut(s)	743
BspPI	GGATC	4 cut(s)	177, 248, 386, 399
BspT104I	TTCGAA	2 cut(s)	125, 927
BspTNI	GGTCTC	1 cut(s)	1153
BsrDI	GCAATG	2 cut(s)	1100, 1280
BsrFI	RCCGGY	2 cut(s)	195, 1285
BsrI	ACTGG	4 cut(s)	45, 644, 714, 1237
BssAI	RCCGGY	2 cut(s)	195, 1285
BssECI	CCNNGG	2 cut(s)	740, 1323
BssT1I	CCWWGG	1 cut(s)	1323
Bst2UI	CCWGG	1 cut(s)	1158
Bst4CI	ACNGT	9 cut(s)	290, 346, 403, 502, 1027, 1228, 1297, 1370, 1544
Bst6I	CTCTTC	1 cut(s)	1027
BstAPI	GCANNNNNTGC	1 cut(s)	1111
BstBAI	YACGTR	1 cut(s)	983
BstBI	TTCGAA	2 cut(s)	125, 927
BstC8I	GCNNGC	3 cut(s)	189, 750, 1588
BstDEI	CTNAG	5 cut(s)	96, 408, 859, 1424, 1554
BstDSI	CCRYGG	1 cut(s)	1323
BstF5I	GGATG	6 cut(s)	220, 431, 435, 479, 696, 1270
BstH2I	RGCGCY	1 cut(s)	898
BstHHI	GCGC	3 cut(s)	304, 897, 1100
BstMAI	GTCTC	2 cut(s)	216, 1153
BstMWI	GCNNNNNNNGC	3 cut(s)	92, 1111, 1596
BstNI	CCWGG	1 cut(s)	1158
BstNSI	RCATGY	1 cut(s)	632
BstSCI	CCNGG	2 cut(s)	256, 1156
BstSFI	CTRYAG	2 cut(s)	90, 750
BstSLI	GKGCMC	1 cut(s)	544
BstV1I	GCAGC	4 cut(s)	73, 513, 732, 1570
BstX2I	RGATCY	2 cut(s)	391, 1054
BstYI	RGATCY	2 cut(s)	391, 1054
BsuRI	GGCC	3 cut(s)	582, 677, 1538
BtgI	CCRYGG	1 cut(s)	1323
BtgZI	GCGATG	1 cut(s)	1396
BtrI	CACGTC	1 cut(s)	1358
BtsCI	GGATG	6 cut(s)	220, 431, 435, 479, 696, 1270
BtsIMutI	CAGTG	8 cut(s)	38, 507, 541, 637, 707, 1230, 1375, 1549
BveI	ACCTGC	1 cut(s)	743
Cac8I	GCNNGC	3 cut(s)	189, 750, 1588
CciI	TCATGA	1 cut(s)	1182
CfoI	GCGC	3 cut(s)	304, 897, 1100
Cfr10I	RCCGGY	2 cut(s)	195, 1285
Cfr13I	GGNCC	2 cut(s)	352, 1361
CseI	GACGC	1 cut(s)	233
CsiI	ACCWGGT	1 cut(s)	1156
Csp6I	GTAC	2 cut(s)	7, 286
CspAI	ACCGGT	1 cut(s)	195
CviQI	GTAC	2 cut(s)	7, 286
DdeI	CTNAG	5 cut(s)	96, 408, 859, 1424, 1554
DraI	TTTAAA	1 cut(s)	54
DraIII	CACNNNGTG	1 cut(s)	643
EaeI	YGGCCR	1 cut(s)	1536
Eam1104I	CTCTTC	1 cut(s)	1027
EarI	CTCTTC	1 cut(s)	1027
Eco130I	CCWWGG	1 cut(s)	1323
Eco147I	AGGCCT	1 cut(s)	677
Eco31I	GGTCTC	1 cut(s)	1153
Eco47I	GGWCC	2 cut(s)	352, 1361
Eco47III	AGCGCT	1 cut(s)	896
Eco57I	CTGAAG	1 cut(s)	648
Eco72I	CACGTG	1 cut(s)	983
Eco88I	CYCGRG	1 cut(s)	739
EcoRII	CCWGG	1 cut(s)	1156
EcoT14I	CCWWGG	1 cut(s)	1323
ErhI	CCWWGG	1 cut(s)	1323
Esp3I	CGTCTC	1 cut(s)	216
FaqI	GGGAC	2 cut(s)	1036, 1306
FbaI	TGATCA	2 cut(s)	607, 1246
Fnu4HI	GCNGC	6 cut(s)	87, 434, 527, 746, 1493, 1584
FokI	GGATG	6 cut(s)	227, 438, 442, 486, 703, 1277
Fsp4HI	GCNGC	6 cut(s)	87, 434, 527, 746, 1493, 1584
FspBI	CTAG	3 cut(s)	717, 1353, 1446
FspI	TGCGCA	1 cut(s)	1099
GlaI	GCGC	3 cut(s)	303, 896, 1099
GluI	GCNGC	6 cut(s)	87, 434, 527, 746, 1493, 1584
GsaI	CCCAGC	3 cut(s)	867, 1023, 1264
GsuI	CTGGAG	1 cut(s)	1254
HaeII	RGCGCY	1 cut(s)	898
HaeIII	GGCC	3 cut(s)	582, 677, 1538
HapII	CCGG	4 cut(s)	196, 257, 1286, 1521
HgaI	GACGC	1 cut(s)	233
HhaI	GCGC	3 cut(s)	304, 897, 1100
Hin6I	GCGC	3 cut(s)	302, 895, 1098
HinP1I	GCGC	3 cut(s)	302, 895, 1098
HincII	GTYRAC	1 cut(s)	1120
HindII	GTYRAC	1 cut(s)	1120
HinfI	GANTC	3 cut(s)	269, 414, 800
HpaII	CCGG	4 cut(s)	196, 257, 1286, 1521
HphI	GGTGA	2 cut(s)	727, 994
Hpy166II	GTNNAC	2 cut(s)	542, 1120
Hpy188I	TCNGA	6 cut(s)	106, 169, 231, 411, 879, 1425
Hpy188III	TCNNGA	7 cut(s)	443, 566, 717, 968, 1141, 1183, 1398
Hpy8I	GTNNAC	2 cut(s)	542, 1120
HpyAV	CCTTC	3 cut(s)	356, 688, 1138
HpyCH4III	ACNGT	9 cut(s)	290, 346, 403, 502, 1027, 1228, 1297, 1370, 1544
HpyCH4IV	ACGT	3 cut(s)	439, 982, 1357
HpyCH4V	TGCA	8 cut(s)	542, 646, 724, 752, 917, 1213, 1280, 1599
HpyF10VI	GCNNNNNNNGC	3 cut(s)	92, 1111, 1596
HpyF3I	CTNAG	5 cut(s)	96, 408, 859, 1424, 1554
HpySE526I	ACGT	3 cut(s)	439, 982, 1357
HspAI	GCGC	3 cut(s)	302, 895, 1098
Ksp22I	TGATCA	2 cut(s)	607, 1246
LmnI	GCTCC	6 cut(s)	232, 299, 1304, 1327, 1523, 1558
Lsp1109I	GCAGC	4 cut(s)	73, 513, 732, 1570
LweI	GCATC	2 cut(s)	420, 441
MabI	ACCWGGT	1 cut(s)	1156
MaeI	CTAG	3 cut(s)	717, 1353, 1446
MaeII	ACGT	3 cut(s)	439, 982, 1357
MaeIII	GTNAC	5 cut(s)	340, 482, 765, 1228, 1348
MboII	GAAGA	7 cut(s)	529, 554, 563, 631, 654, 1044, 1064
MflI	RGATCY	2 cut(s)	391, 1054
MhlI	GDGCHC	3 cut(s)	544, 1107, 1309
MlyI	GAGTC	1 cut(s)	263
MmeI	TCCRAC	1 cut(s)	1044
MseI	TTAA	7 cut(s)	53, 140, 176, 207, 887, 987, 1253
MspA1I	CMGCKG	3 cut(s)	95, 1302, 1553
MspI	CCGG	4 cut(s)	196, 257, 1286, 1521
MspR9I	CCNGG	2 cut(s)	258, 1158
Mva1269I	GAATGC	1 cut(s)	88
MvaI	CCWGG	1 cut(s)	1158
MwoI	GCNNNNNNNGC	3 cut(s)	92, 1111, 1596
NciI	CCSGG	1 cut(s)	258
NcoI	CCATGG	1 cut(s)	1323
NlaIV	GGNNCC	1 cut(s)	393
NmuCI	GTSAC	2 cut(s)	1228, 1348
NsbI	TGCGCA	1 cut(s)	1099
NspI	RCATGY	1 cut(s)	632
NspV	TTCGAA	2 cut(s)	125, 927
PagI	TCATGA	1 cut(s)	1182
PceI	AGGCCT	1 cut(s)	677
PciI	ACATGT	1 cut(s)	628
PctI	GAATGC	1 cut(s)	88
PfeI	GAWTC	2 cut(s)	414, 800
PflFI	GACNNNGTC	1 cut(s)	1359
PflMI	CCANNNNNTGG	2 cut(s)	536, 1370
PinAI	ACCGGT	1 cut(s)	195
PkrI	GCNGC	6 cut(s)	88, 435, 528, 747, 1494, 1585
PleI	GAGTC	1 cut(s)	263
PmaCI	CACGTG	1 cut(s)	983
PmlI	CACGTG	1 cut(s)	983
PpsI	GAGTC	1 cut(s)	263
Ppu21I	YACGTR	1 cut(s)	983
PscI	ACATGT	1 cut(s)	628
Psp6I	CCWGG	1 cut(s)	1156
PspCI	CACGTG	1 cut(s)	983
PspFI	CCCAGC	3 cut(s)	863, 1019, 1260
PspGI	CCWGG	1 cut(s)	1156
PspN4I	GGNNCC	1 cut(s)	393
PspPI	GGNCC	2 cut(s)	352, 1361
PstI	CTGCAG	1 cut(s)	754
PsuI	RGATCY	2 cut(s)	391, 1054
PsyI	GACNNNGTC	1 cut(s)	1359
PvuII	CAGCTG	2 cut(s)	95, 1553
RsaI	GTAC	2 cut(s)	8, 287
RsaNI	GTAC	2 cut(s)	7, 286
SaqAI	TTAA	7 cut(s)	53, 140, 176, 207, 887, 987, 1253
SatI	GCNGC	6 cut(s)	87, 434, 527, 746, 1493, 1584
Sau96I	GGNCC	2 cut(s)	352, 1361
SbfI	CCTGCAGG	1 cut(s)	754
SchI	GAGTC	1 cut(s)	263
ScrFI	CCNGG	2 cut(s)	258, 1158
SdaI	CCTGCAGG	1 cut(s)	754
SduI	GDGCHC	3 cut(s)	544, 1107, 1309
SexAI	ACCWGGT	1 cut(s)	1156
SfaNI	GCATC	2 cut(s)	420, 441
SfcI	CTRYAG	2 cut(s)	90, 750
SfuI	TTCGAA	2 cut(s)	125, 927
SinI	GGWCC	2 cut(s)	352, 1361
Sse8387I	CCTGCAGG	1 cut(s)	754
SseBI	AGGCCT	1 cut(s)	677
SsiI	CCGC	5 cut(s)	283, 434, 1302, 1374, 1492
SspMI	CTAG	3 cut(s)	717, 1353, 1446
StuI	AGGCCT	1 cut(s)	677
StyD4I	CCNGG	2 cut(s)	256, 1156
StyI	CCWWGG	1 cut(s)	1323
TaaI	ACNGT	9 cut(s)	290, 346, 403, 502, 1027, 1228, 1297, 1370, 1544
TaiI	ACGT	3 cut(s)	442, 985, 1360
TaqI	TCGA	5 cut(s)	117, 125, 927, 1009, 1453
TauI	GCSGC	2 cut(s)	436, 1495
TfiI	GAWTC	2 cut(s)	414, 800
Tru1I	TTAA	7 cut(s)	53, 140, 176, 207, 887, 987, 1253
Tru9I	TTAA	7 cut(s)	53, 140, 176, 207, 887, 987, 1253
TscAI	CASTG	8 cut(s)	45, 507, 541, 644, 714, 1237, 1375, 1549
TseFI	GTSAC	2 cut(s)	1228, 1348
TseI	GCWGC	4 cut(s)	86, 526, 745, 1583
Tsp45I	GTSAC	2 cut(s)	1228, 1348
TspDTI	ATGAA	6 cut(s)	563, 707, 1101, 1199, 1380, 1461
TspGWI	ACGGA	2 cut(s)	24, 434
TspRI	CASTG	8 cut(s)	45, 507, 541, 644, 714, 1237, 1375, 1549
Tth111I	GACNNNGTC	1 cut(s)	1359
Van91I	CCANNNNNTGG	2 cut(s)	536, 1370
VneI	GTGCAC	1 cut(s)	540
VpaK11BI	GGWCC	2 cut(s)	352, 1361
XapI	RAATTY	5 cut(s)	25, 136, 930, 972, 1386
XbaI	TCTAGA	1 cut(s)	716
XceI	RCATGY	1 cut(s)	632
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1523
XspI	CTAG	3 cut(s)	717, 1353, 1446

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.