MD01G1045700.v1.1

At5g65660-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004182
Physical Location & Seq
Reverse (-)
14617638 .. 14618605
968 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1045700.v1.1.491

Sequence Viewer

Length: 399 bp
ATGGAGAGCCCGGATTTTTCACCACCCCACGTGGATGCATCACGGCCGTCCTTGGGCTTCCCTCTTGGCACGGCTCTCCTCTTGATCATCATATTTAGCCTCAGTGGAATCTTCTCGTGCTGCTACCACTGGGACAAGCTCAGATCACTCCGTAGATCTTTCAACCAAGACGCTGACCCCGAGGCCGACGTCGAACCGCCATCTCCCGCCAAATCTAAACCTGCCCACATGGATTTGAAGCAGAAGCAGAGGCAAAGCTTGCCAGTGATAATGGCAGGGGACCAGATTCCAAAGTTTATGGCATTGCCATGTCCTCGAGAACCTCCGAGAGATGACAAGATTGCAGTGAAGGTGCAGAAGCAGCCCAAGCCACCTCGATTTCCTGTGCCTCTGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

133

Amino Acids

14.81

Weight (kDa)

9.1

Isoelectric Point (pI)

70.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 192
Acc36I ACCTGC 1 cut(s) 229
AciI CCGC 2 cut(s) 197, 207
AcoI YGGCCR 1 cut(s) 44
AcvI CACGTG 1 cut(s) 31
AcyI GRCGYC 1 cut(s) 189
AfiI CCNNNNNNNGG 1 cut(s) 53
AgsI TTSAA 2 cut(s) 163, 238
AluBI AGCT 2 cut(s) 139, 258
AluI AGCT 2 cut(s) 139, 258
Ama87I CYCGRG 2 cut(s) 179, 315
AoxI GGCC 2 cut(s) 44, 183
ApeKI GCWGC 2 cut(s) 120, 361
AspS9I GGNCC 1 cut(s) 280
AsuC2I CCSGG 1 cut(s) 11
AsuHPI GGTGA 1 cut(s) 12
AvaI CYCGRG 2 cut(s) 179, 315
AvaII GGWCC 1 cut(s) 280
BanII GRGCYC 1 cut(s) 11
BauI CACGAG 1 cut(s) 115
BbrPI CACGTG 1 cut(s) 31
BbvI GCAGC 2 cut(s) 107, 373
BccI CCATC 1 cut(s) 208
BceAI ACGGC 3 cut(s) 31, 59, 87
BclI TGATCA 1 cut(s) 84
BcnI CCSGG 1 cut(s) 11
BfuAI ACCTGC 1 cut(s) 229
BglII AGATCT 1 cut(s) 155
BisI GCNGC 2 cut(s) 121, 362
BlsI GCNGC 2 cut(s) 122, 363
Bme1390I CCNGG 1 cut(s) 11
Bme18I GGWCC 1 cut(s) 280
BmeT110I CYCGRG 2 cut(s) 179, 315
BmgT120I GGNCC 1 cut(s) 280
BmiI GGNNCC 1 cut(s) 281
BmrFI CCNGG 1 cut(s) 11
BmrI ACTGGG 1 cut(s) 139
BmsI GCATC 2 cut(s) 25, 47
BmuI ACTGGG 1 cut(s) 139
BpuMI CCSGG 1 cut(s) 11
BsaAI YACGTR 1 cut(s) 31
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 2 cut(s) 51, 180
Bsc4I CCNNNNNNNGG 1 cut(s) 53
Bse1I ACTGG 2 cut(s) 134, 263
Bse3DI GCAATG 1 cut(s) 302
BseDI CCNNGG 2 cut(s) 51, 180
BseGI GGATG 1 cut(s) 40
BseLI CCNNNNNNNGG 1 cut(s) 53
BseMI GCAATG 1 cut(s) 302
BseMII CTCAG 2 cut(s) 115, 154
BseNI ACTGG 2 cut(s) 134, 263
BseRI GAGGAG 1 cut(s) 68
BseX3I CGGCCG 1 cut(s) 44
BseXI GCAGC 2 cut(s) 107, 373
BsgI GTGCAG 1 cut(s) 374
Bsh1285I CGRYCG 1 cut(s) 47
BshFI GGCC 2 cut(s) 46, 185
BsiEI CGRYCG 1 cut(s) 47
BsiHKCI CYCGRG 2 cut(s) 179, 315
BsiSI CCGG 1 cut(s) 11
BslFI GGGAC 2 cut(s) 146, 293
BslI CCNNNNNNNGG 1 cut(s) 53
BsmFI GGGAC 2 cut(s) 146, 293
BsnI GGCC 2 cut(s) 46, 185
BsoBI CYCGRG 2 cut(s) 179, 315
Bsp1286I GDGCHC 1 cut(s) 11
Bsp143I GATC 3 cut(s) 84, 143, 155
BspACI CCGC 2 cut(s) 197, 207
BspANI GGCC 2 cut(s) 46, 185
BspCNI CTCAG 2 cut(s) 114, 153
BspLI GGNNCC 1 cut(s) 281
BspMI ACCTGC 1 cut(s) 229
BsrDI GCAATG 1 cut(s) 302
BsrI ACTGG 2 cut(s) 134, 263
BssECI CCNNGG 2 cut(s) 51, 180
BssMI GATC 3 cut(s) 84, 143, 155
BssNI GRCGYC 1 cut(s) 189
BssSI CACGAG 1 cut(s) 115
BssT1I CCWWGG 1 cut(s) 51
Bst2BI CACGAG 1 cut(s) 115
BstACI GRCGYC 1 cut(s) 189
BstAPI GCANNNNNTGC 1 cut(s) 259
BstBAI YACGTR 1 cut(s) 31
BstC8I GCNNGC 1 cut(s) 260
BstDEI CTNAG 2 cut(s) 101, 140
BstF5I GGATG 1 cut(s) 40
BstKTI GATC 3 cut(s) 87, 146, 158
BstMBI GATC 3 cut(s) 84, 143, 155
BstMCI CGRYCG 1 cut(s) 47
BstMWI GCNNNNNNNGC 3 cut(s) 259, 361, 367
BstSCI CCNGG 1 cut(s) 9
BstV1I GCAGC 2 cut(s) 107, 373
BstX2I RGATCY 1 cut(s) 155
BstYI RGATCY 1 cut(s) 155
BstZI CGGCCG 1 cut(s) 44
BsuRI GGCC 2 cut(s) 46, 185
BtsCI GGATG 1 cut(s) 40
BtsI GCAGTG 1 cut(s) 351
BtsIMutI CAGTG 4 cut(s) 109, 127, 270, 351
BveI ACCTGC 1 cut(s) 229
Cac8I GCNNGC 1 cut(s) 260
Cfr13I GGNCC 1 cut(s) 280
CseI GACGC 1 cut(s) 179
CviAII CATG 2 cut(s) 229, 309
DdeI CTNAG 2 cut(s) 101, 140
DpnI GATC 3 cut(s) 86, 145, 157
DpnII GATC 3 cut(s) 84, 143, 155
EaeI YGGCCR 1 cut(s) 44
EagI CGGCCG 1 cut(s) 44
EclXI CGGCCG 1 cut(s) 44
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 1 cut(s) 11
Eco47I GGWCC 1 cut(s) 280
Eco52I CGGCCG 1 cut(s) 44
Eco72I CACGTG 1 cut(s) 31
Eco88I CYCGRG 2 cut(s) 179, 315
EcoT14I CCWWGG 1 cut(s) 51
EcoT22I ATGCAT 1 cut(s) 40
EcoT38I GRGCYC 1 cut(s) 11
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 2 cut(s) 232, 312
FaiI YATR 4 cut(s) 92, 230, 299, 310
FaqI GGGAC 2 cut(s) 146, 293
FatI CATG 2 cut(s) 228, 308
FauI CCCGC 1 cut(s) 214
FbaI TGATCA 1 cut(s) 84
Fnu4HI GCNGC 2 cut(s) 121, 362
FokI GGATG 1 cut(s) 47
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 2 cut(s) 121, 362
GluI GCNGC 2 cut(s) 121, 362
HaeIII GGCC 2 cut(s) 46, 185
HapII CCGG 1 cut(s) 11
HgaI GACGC 1 cut(s) 179
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 2 cut(s) 232, 312
HindIII AAGCTT 1 cut(s) 256
HinfI GANTC 2 cut(s) 108, 286
HpaII CCGG 1 cut(s) 11
HphI GGTGA 1 cut(s) 12
Hpy188I TCNGA 2 cut(s) 143, 327
Hpy188III TCNNGA 2 cut(s) 82, 317
Hpy99I CGWCG 2 cut(s) 191, 194
HpyAV CCTTC 1 cut(s) 343
HpyCH4IV ACGT 2 cut(s) 30, 189
HpyCH4V TGCA 3 cut(s) 38, 344, 355
HpyF10VI GCNNNNNNNGC 3 cut(s) 259, 361, 367
HpyF3I CTNAG 2 cut(s) 101, 140
HpySE526I ACGT 2 cut(s) 30, 189
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 2 cut(s) 232, 312
Ksp22I TGATCA 1 cut(s) 84
Kzo9I GATC 3 cut(s) 84, 143, 155
LpnPI CCDG 6 cut(s) 24, 115, 234, 261, 276, 296
Lsp1109I GCAGC 2 cut(s) 107, 373
LweI GCATC 2 cut(s) 25, 47
MaeII ACGT 2 cut(s) 30, 189
MalI GATC 3 cut(s) 86, 145, 157
MboI GATC 3 cut(s) 84, 143, 155
MboII GAAGA 1 cut(s) 103
MflI RGATCY 1 cut(s) 155
MhlI GDGCHC 1 cut(s) 11
MnlI CCTC 9 cut(s) 72, 89, 110, 175, 243, 324, 333, 384, 399
Mph1103I ATGCAT 1 cut(s) 40
MslI CAYNNNNRTG 2 cut(s) 33, 307
MspI CCGG 1 cut(s) 11
MspR9I CCNGG 1 cut(s) 11
MwoI GCNNNNNNNGC 3 cut(s) 259, 361, 367
NciI CCSGG 1 cut(s) 11
NdeII GATC 3 cut(s) 84, 143, 155
NlaIII CATG 2 cut(s) 232, 312
NlaIV GGNNCC 1 cut(s) 281
NsiI ATGCAT 1 cut(s) 40
PaeR7I CTCGAG 1 cut(s) 315
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PfeI GAWTC 2 cut(s) 108, 286
PkrI GCNGC 2 cut(s) 122, 363
PmaCI CACGTG 1 cut(s) 31
PmlI CACGTG 1 cut(s) 31
Ppu21I YACGTR 1 cut(s) 31
PspCI CACGTG 1 cut(s) 31
PspN4I GGNNCC 1 cut(s) 281
PspPI GGNCC 1 cut(s) 280
PsuI RGATCY 1 cut(s) 155
RseI CAYNNNNRTG 2 cut(s) 33, 307
SatI GCNGC 2 cut(s) 121, 362
Sau3AI GATC 3 cut(s) 84, 143, 155
Sau96I GGNCC 1 cut(s) 280
ScrFI CCNGG 1 cut(s) 11
SduI GDGCHC 1 cut(s) 11
SetI ASST 8 cut(s) 33, 141, 192, 223, 260, 325, 354, 376
SfaNI GCATC 2 cut(s) 25, 47
Sfr274I CTCGAG 1 cut(s) 315
SinI GGWCC 1 cut(s) 280
SlaI CTCGAG 1 cut(s) 315
SmiMI CAYNNNNRTG 2 cut(s) 33, 307
SmlI CTYRAG 1 cut(s) 315
SmoI CTYRAG 1 cut(s) 315
SsiI CCGC 2 cut(s) 197, 207
StyD4I CCNGG 1 cut(s) 9
StyI CCWWGG 1 cut(s) 51
TaiI ACGT 2 cut(s) 33, 192
TaqI TCGA 3 cut(s) 192, 316, 376
TfiI GAWTC 2 cut(s) 108, 286
TscAI CASTG 4 cut(s) 109, 134, 270, 351
TseI GCWGC 2 cut(s) 120, 361
TspGWI ACGGA 1 cut(s) 140
TspRI CASTG 4 cut(s) 109, 134, 270, 351
VpaK11BI GGWCC 1 cut(s) 280
XhoI CTCGAG 1 cut(s) 315
ZraI GACGTC 1 cut(s) 190
Zsp2I ATGCAT 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.