Rroxscaffold_2G00133880

At5g65660-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004182
Physical Location & Seq
Reverse (-)
71373206 .. 71373936
731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00133880.1

Sequence Viewer

Length: 414 bp
ATGGAGAGCCAGGATCTTTCGCCACCGCACGTGGACGCGTCTCGGCCGTCCTTGGGGTTCCCTCTCGGCACCGCGCTTCTCCTCATCATCATCTTCAGCTTGAGCGGTGTCTTCTCCTGCTGCTACCACTGGGACAAGCTCAGATCACTCCGCAGGTCTTTCTCCGAAGACTCCACTCCCGAAACCTCCGACGACACCGACGACATCGAAGGCCAGTCTCCCTCCAAATCTAAACCCGCCCCTCTGGACTTGAAACAGAATCAAAAGCAAAGCGTGCCGGTGATAATGCCCGGCGATCAGATTCCAAAGTTTATGGCATTGCCGTGTCCTCGTGAGCCTCCGAGAGAAGAGAAGATAGTTGTCAAAGTTCAAAAACCGCCCAAGCCACCTCGATTTCCTGTACCTCTGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

15.29

Weight (kDa)

6.72

Isoelectric Point (pI)

77.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 144
AccB1I GGYRCC 1 cut(s) 68
AccBSI CCGCTC 1 cut(s) 105
AccII CGCG 2 cut(s) 38, 74
AciI CCGC 6 cut(s) 26, 72, 105, 151, 237, 377
AclWI GGATC 1 cut(s) 21
AcoI YGGCCR 1 cut(s) 44
AcuI CTGAAG 1 cut(s) 79
AcvI CACGTG 1 cut(s) 31
AfaI GTAC 1 cut(s) 402
AfiI CCNNNNNNNGG 1 cut(s) 53
AflIII ACRYGT 1 cut(s) 36
AgsI TTSAA 2 cut(s) 253, 371
AjnI CCWGG 1 cut(s) 9
AluBI AGCT 2 cut(s) 99, 139
AluI AGCT 2 cut(s) 99, 139
Alw26I GTCTC 2 cut(s) 45, 222
AlwI GGATC 1 cut(s) 21
AoxI GGCC 2 cut(s) 44, 211
ApeKI GCWGC 1 cut(s) 120
AspLEI GCGC 1 cut(s) 76
AsuC2I CCSGG 1 cut(s) 291
AsuHPI GGTGA 1 cut(s) 292
BanI GGYRCC 1 cut(s) 68
BauI CACGAG 1 cut(s) 330
BbrPI CACGTG 1 cut(s) 31
BbsI GAAGAC 2 cut(s) 103, 174
BbvI GCAGC 1 cut(s) 107
BceAI ACGGC 2 cut(s) 31, 307
BciT130I CCWGG 1 cut(s) 11
BcnI CCSGG 1 cut(s) 291
BcoDI GTCTC 2 cut(s) 45, 222
BfuAI ACCTGC 1 cut(s) 144
BisI GCNGC 1 cut(s) 121
BlsI GCNGC 1 cut(s) 122
Bme1390I CCNGG 2 cut(s) 11, 291
BmiI GGNNCC 2 cut(s) 59, 70
BmrFI CCNGG 2 cut(s) 11, 291
BmrI ACTGGG 1 cut(s) 139
BmuI ACTGGG 1 cut(s) 139
BpiI GAAGAC 2 cut(s) 103, 174
BpuEI CTTGAG 1 cut(s) 121
BpuMI CCSGG 1 cut(s) 291
BsaAI YACGTR 1 cut(s) 31
BsaJI CCNNGG 1 cut(s) 51
Bsc4I CCNNNNNNNGG 1 cut(s) 53
Bse118I RCCGGY 1 cut(s) 277
Bse1I ACTGG 2 cut(s) 134, 214
Bse3DI GCAATG 1 cut(s) 317
BseBI CCWGG 1 cut(s) 11
BseDI CCNNGG 1 cut(s) 51
BseLI CCNNNNNNNGG 1 cut(s) 53
BseMI GCAATG 1 cut(s) 317
BseMII CTCAG 1 cut(s) 154
BseNI ACTGG 2 cut(s) 134, 214
BseRI GAGGAG 1 cut(s) 71
BseX3I CGGCCG 1 cut(s) 44
BseXI GCAGC 1 cut(s) 107
Bsh1236I CGCG 2 cut(s) 38, 74
Bsh1285I CGRYCG 1 cut(s) 47
BshFI GGCC 2 cut(s) 46, 213
BshNI GGYRCC 1 cut(s) 68
BsiEI CGRYCG 1 cut(s) 47
BsiSI CCGG 2 cut(s) 278, 291
BslFI GGGAC 1 cut(s) 146
BslI CCNNNNNNNGG 1 cut(s) 53
BsmAI GTCTC 2 cut(s) 45, 222
BsmBI CGTCTC 1 cut(s) 45
BsmFI GGGAC 1 cut(s) 146
BsnI GGCC 2 cut(s) 46, 213
Bsp143I GATC 3 cut(s) 13, 143, 295
BspACI CCGC 6 cut(s) 26, 72, 105, 151, 237, 377
BspANI GGCC 2 cut(s) 46, 213
BspCNI CTCAG 1 cut(s) 153
BspFNI CGCG 2 cut(s) 38, 74
BspLI GGNNCC 2 cut(s) 59, 70
BspMI ACCTGC 1 cut(s) 144
BspPI GGATC 1 cut(s) 21
BspT107I GGYRCC 1 cut(s) 68
BsrBI CCGCTC 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 317
BsrFI RCCGGY 1 cut(s) 277
BsrI ACTGG 2 cut(s) 134, 214
BssAI RCCGGY 1 cut(s) 277
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 3 cut(s) 13, 143, 295
BssSI CACGAG 1 cut(s) 330
BssT1I CCWWGG 1 cut(s) 51
Bst2BI CACGAG 1 cut(s) 330
Bst2UI CCWGG 1 cut(s) 11
Bst6I CTCTTC 1 cut(s) 342
BstAPI GCANNNNNTGC 1 cut(s) 274
BstBAI YACGTR 1 cut(s) 31
BstC8I GCNNGC 1 cut(s) 275
BstDEI CTNAG 1 cut(s) 140
BstFNI CGCG 2 cut(s) 38, 74
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 3 cut(s) 16, 146, 298
BstMAI GTCTC 2 cut(s) 45, 222
BstMBI GATC 3 cut(s) 13, 143, 295
BstMCI CGRYCG 1 cut(s) 47
BstMWI GCNNNNNNNGC 1 cut(s) 274
BstNI CCWGG 1 cut(s) 11
BstSCI CCNGG 2 cut(s) 9, 289
BstUI CGCG 2 cut(s) 38, 74
BstV1I GCAGC 1 cut(s) 107
BstV2I GAAGAC 2 cut(s) 103, 174
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
BstZI CGGCCG 1 cut(s) 44
BsuRI GGCC 2 cut(s) 46, 213
BtsIMutI CAGTG 1 cut(s) 127
BveI ACCTGC 1 cut(s) 144
Cac8I GCNNGC 1 cut(s) 275
CfoI GCGC 1 cut(s) 76
Cfr10I RCCGGY 1 cut(s) 277
CseI GACGC 2 cut(s) 27, 44
Csp6I GTAC 1 cut(s) 401
CviJI RGCY 7 cut(s) 9, 46, 99, 139, 213, 337, 385
CviKI_1 RGCY 7 cut(s) 9, 46, 99, 139, 213, 337, 385
CviQI GTAC 1 cut(s) 401
DdeI CTNAG 1 cut(s) 140
DpnI GATC 3 cut(s) 15, 145, 297
DpnII GATC 3 cut(s) 13, 143, 295
EaeI YGGCCR 1 cut(s) 44
EagI CGGCCG 1 cut(s) 44
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
EclXI CGGCCG 1 cut(s) 44
Eco130I CCWWGG 1 cut(s) 51
Eco52I CGGCCG 1 cut(s) 44
Eco57I CTGAAG 1 cut(s) 79
Eco72I CACGTG 1 cut(s) 31
EcoRII CCWGG 1 cut(s) 9
EcoT14I CCWWGG 1 cut(s) 51
ErhI CCWWGG 1 cut(s) 51
Esp3I CGTCTC 1 cut(s) 45
FaiI YATR 1 cut(s) 314
FaqI GGGAC 1 cut(s) 146
FauI CCCGC 1 cut(s) 244
Fnu4HI GCNGC 1 cut(s) 121
Fsp4HI GCNGC 1 cut(s) 121
GlaI GCGC 1 cut(s) 75
GluI GCNGC 1 cut(s) 121
HaeIII GGCC 2 cut(s) 46, 213
HapII CCGG 2 cut(s) 278, 291
HgaI GACGC 2 cut(s) 27, 44
HhaI GCGC 1 cut(s) 76
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HinfI GANTC 3 cut(s) 170, 259, 301
HpaII CCGG 2 cut(s) 278, 291
HphI GGTGA 1 cut(s) 292
Hpy166II GTNNAC 1 cut(s) 34
Hpy188I TCNGA 5 cut(s) 143, 166, 190, 300, 342
Hpy188III TCNNGA 3 cut(s) 179, 245, 332
Hpy8I GTNNAC 1 cut(s) 34
Hpy99I CGWCG 2 cut(s) 194, 203
HpyAV CCTTC 1 cut(s) 203
HpyCH4IV ACGT 1 cut(s) 30
HpyF10VI GCNNNNNNNGC 1 cut(s) 274
HpyF3I CTNAG 1 cut(s) 140
HpySE526I ACGT 1 cut(s) 30
HspAI GCGC 1 cut(s) 74
Kzo9I GATC 3 cut(s) 13, 143, 295
LpnPI CCDG 8 cut(s) 23, 115, 130, 139, 227, 230, 291, 304
Lsp1109I GCAGC 1 cut(s) 107
MaeII ACGT 1 cut(s) 30
MalI GATC 3 cut(s) 15, 145, 297
MbiI CCGCTC 1 cut(s) 105
MboI GATC 3 cut(s) 13, 143, 295
MboII GAAGA 5 cut(s) 85, 103, 179, 359, 364
MflI RGATCY 1 cut(s) 13
MluI ACGCGT 1 cut(s) 36
MlyI GAGTC 1 cut(s) 164
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 9 cut(s) 72, 92, 196, 232, 252, 339, 348, 399, 414
MslI CAYNNNNRTG 1 cut(s) 322
MspI CCGG 2 cut(s) 278, 291
MspR9I CCNGG 2 cut(s) 11, 291
MvaI CCWGG 1 cut(s) 11
MvnI CGCG 2 cut(s) 38, 74
MwoI GCNNNNNNNGC 1 cut(s) 274
NciI CCSGG 1 cut(s) 291
NdeII GATC 3 cut(s) 13, 143, 295
NlaIV GGNNCC 2 cut(s) 59, 70
NmeAIII GCCGAG 2 cut(s) 22, 45
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PfeI GAWTC 2 cut(s) 259, 301
PkrI GCNGC 1 cut(s) 122
PleI GAGTC 1 cut(s) 164
PmaCI CACGTG 1 cut(s) 31
PmlI CACGTG 1 cut(s) 31
PpsI GAGTC 1 cut(s) 164
Ppu21I YACGTR 1 cut(s) 31
Psp6I CCWGG 1 cut(s) 9
PspCI CACGTG 1 cut(s) 31
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 2 cut(s) 59, 70
PsuI RGATCY 1 cut(s) 13
RsaI GTAC 1 cut(s) 402
RsaNI GTAC 1 cut(s) 401
RseI CAYNNNNRTG 1 cut(s) 322
SatI GCNGC 1 cut(s) 121
Sau3AI GATC 3 cut(s) 13, 143, 295
SchI GAGTC 1 cut(s) 164
ScrFI CCNGG 2 cut(s) 11, 291
SetI ASST 7 cut(s) 33, 101, 141, 158, 188, 391, 406
SmiMI CAYNNNNRTG 1 cut(s) 322
SmlI CTYRAG 1 cut(s) 100
SmoI CTYRAG 1 cut(s) 100
SsiI CCGC 6 cut(s) 26, 72, 105, 151, 237, 377
StyD4I CCNGG 2 cut(s) 9, 289
StyI CCWWGG 1 cut(s) 51
TaiI ACGT 1 cut(s) 33
TaqI TCGA 2 cut(s) 207, 391
TfiI GAWTC 2 cut(s) 259, 301
TscAI CASTG 1 cut(s) 134
TseI GCWGC 1 cut(s) 120
TspRI CASTG 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.