Rorug02G0163800

At5g65660-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004182
Physical Location & Seq
Reverse (-)
14603328 .. 14606598
3271 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0163800.1

Sequence Viewer

Length: 894 bp
ATGGCCAAATCCTCCTACCAAACCTACCGGCGCCGAACTAGGAGCTACCGGAACTGGCTGGAGCTCCCGGACGACGTCACCGCTTCCATACTCTCCCGCCTCGGCGCCATAGAAATCCTGACGCGAGCTCAGAAGGTCTGCATGCCGTGGCGCAAAATCTGCAAGGACCGTTTGATGTGGCGCACCATCGACATGAGAAACGACGGTTTTCTCCGCGATTACGACTTCGACTTGGACAAGATGTGCCGCCACGGCGTTGATCGGAGCTCCGGCGGTCTCGTCGACATCAATGTCGAACACTTCGGCACCGATGAGCTCCTCAAGTATATCACTAATAGTTCAACTGGAATTAGACGCCTCCGTTTAGTCTGTTGTTATGGCATAACCGATGAGGGGTTGATTGAAGTGGCTTCGAAACTTCCGCTGCTGGAGCACCTTGAAATTTCTTTCAGCTTTGCCTCTGCTCAATCTTTGGAAGTGATTGGGCGCTCTTGCCCTCTTTTGAAATCACTCAAACTGAACAAATATGGGTACAGATGCCCGTATCATGAGTGCGATGATGATGCACTTGCTATAGCGTCCACAATGCATGATCTACACCACCTGCAGCTTTGCGGTAATAAGCTGACCAAAGATGGCTTGCAGAAAATCCTTGATTGTTGTCCAAATCTTGAGTCACTTGATCTGCGCAAGTGTTTCAATATCAATTTCGGGGTAGTTTCGAAAAGAAGATGCAGTAAACGAATTAAAAGTTTACGGCTTCCTGATGATTCCACCGATGACTATGAGTTTGATCGTGCAAATCGTAGGGAGTTATATGCTCAAACCCATTGCGACCCAGATTATGGTGACTTCCTCCTATTCGGACGTTCTGTTTTTGGTCGAGATTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

297

Amino Acids

34.42

Weight (kDa)

8.33

Isoelectric Point (pI)

51.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 19 - 65 5.5e-09 F-box-like
F-box PF00646 19 - 61 1.9e-06 F-box domain
LRR_At5g56370 PF24758 184 - 257 1.3e-06 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 612
AasI GACNNNNNNGTC 1 cut(s) 290
AatII GACGTC 1 cut(s) 78
Acc16I TGCGCA 1 cut(s) 689
Acc36I ACCTGC 1 cut(s) 612
AccB1I GGYRCC 3 cut(s) 30, 104, 305
AccB7I CCANNNNNTGG 1 cut(s) 845
AccI GTMKAC 1 cut(s) 282
AccII CGCG 2 cut(s) 124, 216
AciI CCGC 7 cut(s) 81, 97, 214, 247, 273, 422, 615
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 441
AcyI GRCGYC 4 cut(s) 31, 75, 105, 355
AfaI GTAC 1 cut(s) 533
AfiI CCNNNNNNNGG 2 cut(s) 393, 845
AgsI TTSAA 5 cut(s) 342, 404, 440, 505, 700
AluBI AGCT 8 cut(s) 45, 64, 128, 267, 316, 453, 610, 625
AluI AGCT 8 cut(s) 45, 64, 128, 267, 316, 453, 610, 625
Alw21I GWGCWC 5 cut(s) 66, 130, 269, 318, 435
Alw26I GTCTC 1 cut(s) 281
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 424, 607
ApoI RAATTY 1 cut(s) 441
ArsI GACNNNNNNTTYG 4 cut(s) 659, 691, 773, 805
AspLEI GCGC 6 cut(s) 33, 107, 153, 183, 489, 690
AspS9I GGNCC 1 cut(s) 166
AsuC2I CCSGG 1 cut(s) 68
AsuHPI GGTGA 2 cut(s) 70, 860
AsuII TTCGAA 2 cut(s) 413, 722
AvaII GGWCC 1 cut(s) 166
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 3 cut(s) 30, 104, 305
BanII GRGCYC 4 cut(s) 66, 130, 269, 318
Bbv12I GWGCWC 5 cut(s) 66, 130, 269, 318, 435
BbvI GCAGC 2 cut(s) 411, 619
BccI CCATC 2 cut(s) 194, 629
BceAI ACGGC 3 cut(s) 130, 268, 773
BcgI CGANNNNNNTGC 2 cut(s) 545, 579
BcnI CCSGG 1 cut(s) 68
BcoDI GTCTC 1 cut(s) 281
BfaI CTAG 1 cut(s) 39
BfmI CTRYAG 2 cut(s) 573, 605
BfoI RGCGCY 3 cut(s) 34, 108, 490
BfuAI ACCTGC 1 cut(s) 612
BglI GCCNNNNNGGC 1 cut(s) 252
BisI GCNGC 3 cut(s) 247, 425, 608
BlsI GCNGC 3 cut(s) 248, 426, 609
Bme1390I CCNGG 1 cut(s) 68
Bme18I GGWCC 1 cut(s) 166
BmgT120I GGNCC 1 cut(s) 166
BmiI GGNNCC 3 cut(s) 32, 106, 307
BmrFI CCNGG 1 cut(s) 68
BmsI GCATC 3 cut(s) 527, 553, 722
BpmI CTGGAG 2 cut(s) 80, 449
Bpu14I TTCGAA 2 cut(s) 413, 722
BpuEI CTTGAG 2 cut(s) 305, 692
BpuMI CCSGG 1 cut(s) 68
BsaHI GRCGYC 4 cut(s) 31, 75, 105, 355
BsaI GGTCTC 1 cut(s) 281
BsaJI CCNNGG 3 cut(s) 100, 146, 250
BsaWI WCCGGW 1 cut(s) 48
Bsc4I CCNNNNNNNGG 2 cut(s) 393, 845
Bse118I RCCGGY 1 cut(s) 27
Bse1I ACTGG 2 cut(s) 59, 349
Bse3DI GCAATG 1 cut(s) 829
BseDI CCNNGG 3 cut(s) 100, 146, 250
BseLI CCNNNNNNNGG 2 cut(s) 393, 845
BseMI GCAATG 1 cut(s) 829
BseMII CTCAG 1 cut(s) 143
BseNI ACTGG 2 cut(s) 59, 349
BseRI GAGGAG 1 cut(s) 308
BseXI GCAGC 2 cut(s) 411, 619
Bsh1236I CGCG 2 cut(s) 124, 216
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 3 cut(s) 30, 104, 305
BsiHKAI GWGCWC 5 cut(s) 66, 130, 269, 318, 435
BsiSI CCGG 4 cut(s) 28, 49, 68, 270
BslI CCNNNNNNNGG 2 cut(s) 393, 845
BsmAI GTCTC 1 cut(s) 281
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 281
Bsp119I TTCGAA 2 cut(s) 413, 722
Bsp1286I GDGCHC 5 cut(s) 66, 130, 269, 318, 435
Bsp143I GATC 4 cut(s) 259, 592, 682, 793
BspACI CCGC 7 cut(s) 81, 97, 214, 247, 273, 422, 615
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 142
BspFNI CGCG 2 cut(s) 124, 216
BspHI TCATGA 1 cut(s) 547
BspLI GGNNCC 3 cut(s) 32, 106, 307
BspMAI CTGCAG 1 cut(s) 609
BspMI ACCTGC 1 cut(s) 612
BspT104I TTCGAA 2 cut(s) 413, 722
BspT107I GGYRCC 3 cut(s) 30, 104, 305
BspTNI GGTCTC 1 cut(s) 281
BsrDI GCAATG 1 cut(s) 829
BsrFI RCCGGY 1 cut(s) 27
BsrI ACTGG 2 cut(s) 59, 349
BssAI RCCGGY 1 cut(s) 27
BssECI CCNNGG 3 cut(s) 100, 146, 250
BssMI GATC 4 cut(s) 259, 592, 682, 793
BssNI GRCGYC 4 cut(s) 31, 75, 105, 355
Bst4CI ACNGT 2 cut(s) 170, 206
BstACI GRCGYC 4 cut(s) 31, 75, 105, 355
BstAPI GCANNNNNTGC 1 cut(s) 159
BstBI TTCGAA 2 cut(s) 413, 722
BstC8I GCNNGC 3 cut(s) 126, 143, 641
BstDEI CTNAG 1 cut(s) 129
BstDSI CCRYGG 2 cut(s) 146, 250
BstFNI CGCG 2 cut(s) 124, 216
BstH2I RGCGCY 3 cut(s) 34, 108, 490
BstHHI GCGC 6 cut(s) 33, 107, 153, 183, 489, 690
BstKTI GATC 4 cut(s) 262, 595, 685, 796
BstMAI GTCTC 1 cut(s) 281
BstMBI GATC 4 cut(s) 259, 592, 682, 793
BstMWI GCNNNNNNNGC 3 cut(s) 159, 252, 430
BstNSI RCATGY 1 cut(s) 145
BstSCI CCNGG 1 cut(s) 66
BstSFI CTRYAG 2 cut(s) 573, 605
BstUI CGCG 2 cut(s) 124, 216
BstV1I GCAGC 2 cut(s) 411, 619
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 2 cut(s) 146, 250
BtgZI GCGATG 1 cut(s) 570
BveI ACCTGC 1 cut(s) 612
Cac8I GCNNGC 3 cut(s) 126, 143, 641
CciI TCATGA 1 cut(s) 547
CfoI GCGC 6 cut(s) 33, 107, 153, 183, 489, 690
Cfr10I RCCGGY 1 cut(s) 27
Cfr13I GGNCC 1 cut(s) 166
CseI GACGC 3 cut(s) 130, 363, 567
Csp6I GTAC 1 cut(s) 532
CviAII CATG 4 cut(s) 142, 193, 548, 590
CviQI GTAC 1 cut(s) 532
DdeI CTNAG 1 cut(s) 129
DinI GGCGCC 2 cut(s) 32, 106
DpnI GATC 4 cut(s) 261, 594, 684, 795
DpnII GATC 4 cut(s) 259, 592, 682, 793
DrdI GACNNNNNNGTC 1 cut(s) 290
DseDI GACNNNNNNGTC 1 cut(s) 290
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 4 cut(s) 64, 128, 267, 316
Eco24I GRGCYC 4 cut(s) 66, 130, 269, 318
Eco31I GGTCTC 1 cut(s) 281
Eco47I GGWCC 1 cut(s) 166
Eco53kI GAGCTC 4 cut(s) 64, 128, 267, 316
EcoICRI GAGCTC 4 cut(s) 64, 128, 267, 316
EcoT22I ATGCAT 1 cut(s) 591
EcoT38I GRGCYC 4 cut(s) 66, 130, 269, 318
EgeI GGCGCC 2 cut(s) 32, 106
EheI GGCGCC 2 cut(s) 32, 106
FaeI CATG 4 cut(s) 145, 196, 551, 593
FatI CATG 4 cut(s) 141, 192, 547, 589
FauI CCCGC 1 cut(s) 104
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 3 cut(s) 247, 425, 608
FriOI GRGCYC 4 cut(s) 66, 130, 269, 318
Fsp4HI GCNGC 3 cut(s) 247, 425, 608
FspBI CTAG 1 cut(s) 39
FspI TGCGCA 1 cut(s) 689
GlaI GCGC 6 cut(s) 32, 106, 152, 182, 488, 689
GluI GCNGC 3 cut(s) 247, 425, 608
GsuI CTGGAG 2 cut(s) 80, 449
HaeII RGCGCY 3 cut(s) 34, 108, 490
HaeIII GGCC 1 cut(s) 5
HapII CCGG 4 cut(s) 28, 49, 68, 270
HgaI GACGC 3 cut(s) 130, 363, 567
HhaI GCGC 6 cut(s) 33, 107, 153, 183, 489, 690
Hin1I GRCGYC 4 cut(s) 31, 75, 105, 355
Hin1II CATG 4 cut(s) 145, 196, 551, 593
Hin6I GCGC 6 cut(s) 31, 105, 151, 181, 487, 688
HinP1I GCGC 6 cut(s) 31, 105, 151, 181, 487, 688
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HinfI GANTC 2 cut(s) 674, 770
HpaII CCGG 4 cut(s) 28, 49, 68, 270
HphI GGTGA 2 cut(s) 70, 860
Hpy166II GTNNAC 4 cut(s) 283, 582, 740, 755
Hpy188I TCNGA 3 cut(s) 132, 264, 866
Hpy188III TCNNGA 5 cut(s) 118, 548, 671, 764, 884
Hpy8I GTNNAC 4 cut(s) 283, 582, 740, 755
Hpy99I CGWCG 3 cut(s) 77, 206, 284
HpyAV CCTTC 1 cut(s) 127
HpyCH4III ACNGT 2 cut(s) 170, 206
HpyCH4IV ACGT 2 cut(s) 75, 868
HpyCH4V TGCA 8 cut(s) 141, 162, 566, 589, 607, 643, 735, 800
HpyF10VI GCNNNNNNNGC 3 cut(s) 159, 252, 430
HpyF3I CTNAG 1 cut(s) 129
HpySE526I ACGT 2 cut(s) 75, 868
Hsp92I GRCGYC 4 cut(s) 31, 75, 105, 355
Hsp92II CATG 4 cut(s) 145, 196, 551, 593
HspAI GCGC 6 cut(s) 31, 105, 151, 181, 487, 688
KasI GGCGCC 2 cut(s) 30, 104
Kzo9I GATC 4 cut(s) 259, 592, 682, 793
LmnI GCTCC 7 cut(s) 42, 61, 69, 264, 272, 321, 430
Lsp1109I GCAGC 2 cut(s) 411, 619
LweI GCATC 3 cut(s) 527, 553, 722
MaeI CTAG 1 cut(s) 39
MaeII ACGT 2 cut(s) 75, 868
MaeIII GTNAC 3 cut(s) 76, 675, 848
MalI GATC 4 cut(s) 261, 594, 684, 795
MboI GATC 4 cut(s) 259, 592, 682, 793
MboII GAAGA 1 cut(s) 741
MhlI GDGCHC 5 cut(s) 66, 130, 269, 318, 435
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 348, 441, 706, 744
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 2 cut(s) 31, 105
MlyI GAGTC 1 cut(s) 683
MnlI CCTC 8 cut(s) 22, 110, 329, 368, 385, 469, 507, 866
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 591
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 747
MslI CAYNNNNRTG 1 cut(s) 191
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 424
MspI CCGG 4 cut(s) 28, 49, 68, 270
MspR9I CCNGG 1 cut(s) 68
MvnI CGCG 2 cut(s) 124, 216
MwoI GCNNNNNNNGC 3 cut(s) 159, 252, 430
NarI GGCGCC 2 cut(s) 31, 105
NciI CCSGG 1 cut(s) 68
NdeII GATC 4 cut(s) 259, 592, 682, 793
NlaIII CATG 4 cut(s) 145, 196, 551, 593
NlaIV GGNNCC 3 cut(s) 32, 106, 307
NmeAIII GCCGAG 1 cut(s) 81
NmuCI GTSAC 3 cut(s) 76, 675, 848
NsbI TGCGCA 1 cut(s) 689
NsiI ATGCAT 1 cut(s) 591
NspI RCATGY 1 cut(s) 145
NspV TTCGAA 2 cut(s) 413, 722
PaeI GCATGC 1 cut(s) 145
PagI TCATGA 1 cut(s) 547
PaqCI CACCTGC 1 cut(s) 612
PcsI WCGNNNNNNNCGW 1 cut(s) 802
PfeI GAWTC 1 cut(s) 770
PflFI GACNNNGTC 1 cut(s) 74
PflMI CCANNNNNTGG 1 cut(s) 845
PfoI TCCNGGA 1 cut(s) 66
PkrI GCNGC 3 cut(s) 248, 426, 609
PleI GAGTC 1 cut(s) 682
PluTI GGCGCC 2 cut(s) 34, 108
PpsI GAGTC 1 cut(s) 682
Psp124BI GAGCTC 4 cut(s) 66, 130, 269, 318
PspN4I GGNNCC 3 cut(s) 32, 106, 307
PspPI GGNCC 1 cut(s) 166
PstI CTGCAG 1 cut(s) 609
PsyI GACNNNGTC 1 cut(s) 74
RsaI GTAC 1 cut(s) 533
RsaNI GTAC 1 cut(s) 532
RseI CAYNNNNRTG 1 cut(s) 191
SacI GAGCTC 4 cut(s) 66, 130, 269, 318
SalI GTCGAC 1 cut(s) 281
SaqAI TTAA 1 cut(s) 747
SatI GCNGC 3 cut(s) 247, 425, 608
Sau3AI GATC 4 cut(s) 259, 592, 682, 793
Sau96I GGNCC 1 cut(s) 166
SchI GAGTC 1 cut(s) 683
ScrFI CCNGG 1 cut(s) 68
SduI GDGCHC 5 cut(s) 66, 130, 269, 318, 435
SfaNI GCATC 3 cut(s) 527, 553, 722
SfcI CTRYAG 2 cut(s) 573, 605
SfoI GGCGCC 2 cut(s) 32, 106
SfuI TTCGAA 2 cut(s) 413, 722
SinI GGWCC 1 cut(s) 166
SmiMI CAYNNNNRTG 1 cut(s) 191
SmlI CTYRAG 2 cut(s) 320, 671
SmoI CTYRAG 2 cut(s) 320, 671
SphI GCATGC 1 cut(s) 145
Sse9I AATT 4 cut(s) 348, 441, 706, 744
SsiI CCGC 7 cut(s) 81, 97, 214, 247, 273, 422, 615
SspDI GGCGCC 2 cut(s) 30, 104
SspMI CTAG 1 cut(s) 39
SstI GAGCTC 4 cut(s) 66, 130, 269, 318
StyD4I CCNGG 1 cut(s) 66
TaaI ACNGT 2 cut(s) 170, 206
TaiI ACGT 2 cut(s) 78, 871
TaqI TCGA 7 cut(s) 189, 228, 282, 294, 413, 722, 883
TasI AATT 4 cut(s) 348, 441, 706, 744
TauI GCSGC 1 cut(s) 249
TfiI GAWTC 1 cut(s) 770
Tru1I TTAA 1 cut(s) 747
Tru9I TTAA 1 cut(s) 747
TseFI GTSAC 3 cut(s) 76, 675, 848
TseI GCWGC 2 cut(s) 424, 607
Tsp45I GTSAC 3 cut(s) 76, 675, 848
TspGWI ACGGA 1 cut(s) 350
Tth111I GACNNNGTC 1 cut(s) 74
Van91I CCANNNNNTGG 1 cut(s) 845
VpaK11BI GGWCC 1 cut(s) 166
XapI RAATTY 1 cut(s) 441
XceI RCATGY 1 cut(s) 145
XmiI GTMKAC 1 cut(s) 282
XspI CTAG 1 cut(s) 39
ZraI GACGTC 1 cut(s) 76
Zsp2I ATGCAT 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.