pycom15g35270

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0004182
Physical Location & Seq
Reverse (-)
35169524 .. 35169985
462 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g35270.1

Sequence Viewer

Length: 363 bp
ATGGAGCGAAACGATGAAGTTTCAACTCGGCCATCGCTAGCTTTTCCTGTTAGCTTGGCGCTGCTTGTGTTGCTGTTATTGAGCGCCAGCATCTTCTTCTTGTGTTGCCTCCAATGGGACAAGCTTCGGTCTCTATTTCTGTCCTCTGTGGAAGAAGGCAACCCTGACGATCATATCCTACCAGATTTAGATCAGAAATCTACTCGTCCAAACATGATGGAAAAGCAAAATCAGCAGAAGAGCCTGACAGTGCTGATGCCAGGAGATCAGGTGCCAAAGTTTATAGCAATTGCTTGTCCATGTCAGGCTCCAGTAATGGAGAAGCTCACTGTGATTGTGCAGAAGCCTACCCTTTTTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

121

Amino Acids

13.49

Weight (kDa)

5.28

Isoelectric Point (pI)

48.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 271
AcoI YGGCCR 1 cut(s) 29
AfiI CCNNNNNNNGG 1 cut(s) 115
AgsI TTSAA 1 cut(s) 24
AjnI CCWGG 1 cut(s) 259
AluBI AGCT 4 cut(s) 41, 54, 124, 325
AluI AGCT 4 cut(s) 41, 54, 124, 325
Alw26I GTCTC 1 cut(s) 135
AoxI GGCC 1 cut(s) 29
ApeKI GCWGC 1 cut(s) 61
AspLEI GCGC 2 cut(s) 61, 86
AsuNHI GCTAGC 1 cut(s) 37
BanI GGYRCC 1 cut(s) 271
BbvI GCAGC 1 cut(s) 48
BccI CCATC 2 cut(s) 40, 211
BciT130I CCWGG 1 cut(s) 261
BcoDI GTCTC 1 cut(s) 135
BfaI CTAG 1 cut(s) 38
BfoI RGCGCY 2 cut(s) 62, 87
BisI GCNGC 1 cut(s) 62
BlsI GCNGC 1 cut(s) 63
Bme1390I CCNGG 1 cut(s) 261
BmiI GGNNCC 2 cut(s) 273, 309
BmrFI CCNGG 1 cut(s) 261
BmsI GCATC 2 cut(s) 99, 246
BmtI GCTAGC 1 cut(s) 41
BpmI CTGGAG 1 cut(s) 294
BsaBI GATNNNNATC 1 cut(s) 189
BsaI GGTCTC 1 cut(s) 135
BsaXI ACNNNNNCTCC 2 cut(s) 255, 285
Bsc4I CCNNNNNNNGG 1 cut(s) 115
Bse1I ACTGG 1 cut(s) 311
Bse8I GATNNNNATC 1 cut(s) 189
BseBI CCWGG 1 cut(s) 261
BseJI GATNNNNATC 1 cut(s) 189
BseLI CCNNNNNNNGG 1 cut(s) 115
BseNI ACTGG 1 cut(s) 311
BseXI GCAGC 1 cut(s) 48
BsgI GTGCAG 1 cut(s) 359
BshFI GGCC 1 cut(s) 31
BshNI GGYRCC 1 cut(s) 271
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 1 cut(s) 115
BsmAI GTCTC 1 cut(s) 135
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 1 cut(s) 31
Bso31I GGTCTC 1 cut(s) 135
Bsp143I GATC 3 cut(s) 169, 190, 265
BspANI GGCC 1 cut(s) 31
BspLI GGNNCC 2 cut(s) 273, 309
BspOI GCTAGC 1 cut(s) 41
BspQI GCTCTTC 1 cut(s) 233
BspT107I GGYRCC 1 cut(s) 271
BspTNI GGTCTC 1 cut(s) 135
BsrI ACTGG 1 cut(s) 311
BssMI GATC 3 cut(s) 169, 190, 265
Bst2UI CCWGG 1 cut(s) 261
Bst4CI ACNGT 2 cut(s) 250, 331
Bst6I CTCTTC 1 cut(s) 233
BstC8I GCNNGC 2 cut(s) 39, 88
BstH2I RGCGCY 2 cut(s) 62, 87
BstHHI GCGC 2 cut(s) 61, 86
BstKTI GATC 3 cut(s) 172, 193, 268
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 3 cut(s) 169, 190, 265
BstMWI GCNNNNNNNGC 2 cut(s) 70, 232
BstNI CCWGG 1 cut(s) 261
BstSCI CCNGG 1 cut(s) 259
BstV1I GCAGC 1 cut(s) 48
BsuRI GGCC 1 cut(s) 31
BtgZI GCGATG 1 cut(s) 18
BtsIMutI CAGTG 2 cut(s) 255, 327
Cac8I GCNNGC 2 cut(s) 39, 88
CfoI GCGC 2 cut(s) 61, 86
CviAII CATG 2 cut(s) 214, 300
CviJI RGCY 8 cut(s) 31, 41, 54, 124, 243, 308, 325, 346
CviKI_1 RGCY 8 cut(s) 31, 41, 54, 124, 243, 308, 325, 346
DpnI GATC 3 cut(s) 171, 192, 267
DpnII GATC 3 cut(s) 169, 190, 265
EaeI YGGCCR 1 cut(s) 29
Eam1104I CTCTTC 1 cut(s) 233
EarI CTCTTC 1 cut(s) 233
Eco31I GGTCTC 1 cut(s) 135
EcoRII CCWGG 1 cut(s) 259
FaeI CATG 2 cut(s) 217, 303
FaiI YATR 4 cut(s) 174, 215, 284, 301
FaqI GGGAC 1 cut(s) 131
FatI CATG 2 cut(s) 213, 299
Fnu4HI GCNGC 1 cut(s) 62
Fsp4HI GCNGC 1 cut(s) 62
FspBI CTAG 1 cut(s) 38
GlaI GCGC 2 cut(s) 60, 85
GluI GCNGC 1 cut(s) 62
GsuI CTGGAG 1 cut(s) 294
HaeII RGCGCY 2 cut(s) 62, 87
HaeIII GGCC 1 cut(s) 31
HhaI GCGC 2 cut(s) 61, 86
Hin1II CATG 2 cut(s) 217, 303
Hin6I GCGC 2 cut(s) 59, 84
HinP1I GCGC 2 cut(s) 59, 84
HindIII AAGCTT 1 cut(s) 122
Hpy188I TCNGA 1 cut(s) 195
HpyAV CCTTC 1 cut(s) 149
HpyCH4III ACNGT 2 cut(s) 250, 331
HpyCH4V TGCA 1 cut(s) 340
HpyF10VI GCNNNNNNNGC 2 cut(s) 70, 232
Hsp92II CATG 2 cut(s) 217, 303
HspAI GCGC 2 cut(s) 59, 84
Kzo9I GATC 3 cut(s) 169, 190, 265
LguI GCTCTTC 1 cut(s) 233
LmnI GCTCC 2 cut(s) 4, 313
Lsp1109I GCAGC 1 cut(s) 48
LweI GCATC 2 cut(s) 99, 246
MaeI CTAG 1 cut(s) 38
MalI GATC 3 cut(s) 171, 192, 267
MboI GATC 3 cut(s) 169, 190, 265
MboII GAAGA 4 cut(s) 85, 88, 164, 250
MfeI CAATTG 1 cut(s) 288
MluCI AATT 1 cut(s) 288
MnlI CCTC 2 cut(s) 119, 154
MseI TTAA 1 cut(s) 361
MspR9I CCNGG 1 cut(s) 261
MunI CAATTG 1 cut(s) 288
MvaI CCWGG 1 cut(s) 261
MwoI GCNNNNNNNGC 2 cut(s) 70, 232
NdeII GATC 3 cut(s) 169, 190, 265
NheI GCTAGC 1 cut(s) 37
NlaIII CATG 2 cut(s) 217, 303
NlaIV GGNNCC 2 cut(s) 273, 309
NmeAIII GCCGAG 1 cut(s) 7
PciSI GCTCTTC 1 cut(s) 233
PkrI GCNGC 1 cut(s) 63
Psp6I CCWGG 1 cut(s) 259
PspGI CCWGG 1 cut(s) 259
PspN4I GGNNCC 2 cut(s) 273, 309
SapI GCTCTTC 1 cut(s) 233
SaqAI TTAA 1 cut(s) 361
SatI GCNGC 1 cut(s) 62
Sau3AI GATC 3 cut(s) 169, 190, 265
ScrFI CCNGG 1 cut(s) 261
SetI ASST 5 cut(s) 43, 56, 126, 273, 327
SfaNI GCATC 2 cut(s) 99, 246
Sse9I AATT 1 cut(s) 288
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 1 cut(s) 259
TaaI ACNGT 2 cut(s) 250, 331
TaqII GACCGA 1 cut(s) 117
TasI AATT 1 cut(s) 288
Tru1I TTAA 1 cut(s) 361
Tru9I TTAA 1 cut(s) 361
TscAI CASTG 2 cut(s) 255, 334
TseI GCWGC 1 cut(s) 61
TspDTI ATGAA 1 cut(s) 30
TspRI CASTG 2 cut(s) 255, 334
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.