MD08G1207400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0004182
Physical Location & Seq
Reverse (-)
26976170 .. 26976659
490 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1207400.v1.1.491

Sequence Viewer

Length: 360 bp
ATGGAGCAAAACGACGAAGTTTCAACTCCATCTCTAGATTTTCCTGTTGGCTTGGCGCTGCTTCTGTTGCTGTTATTGAGCGTCAGTACCTTCTTCTTGTGTTGCCTTCATTGGGACAAGGTTCGGTCTCTATTTCTATCCTCTGTGGAAGAAGGCAACCCTGACGATCATATCCTGCCAGACTTTGATCAGAAACCTACTCGTCCAAACATGATGGAAAAGCAAAATCAGCAGAAGAGCCTGACAGTGCTGATGCCTGGAGAACAGGTGCCCAAGTTCATAGCAATTGCTTGTCCATGTCAGCCTCCGGTAATGGAGAAGCTTTCTGTGGTTGTGCAGAAGCCTACCCTTTTTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

120

Amino Acids

13.41

Weight (kDa)

4.82

Isoelectric Point (pI)

46.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 268
AfaI GTAC 1 cut(s) 88
AfiI CCNNNNNNNGG 1 cut(s) 112
AgsI TTSAA 1 cut(s) 24
AjnI CCWGG 1 cut(s) 256
AluBI AGCT 1 cut(s) 322
AluI AGCT 1 cut(s) 322
Alw26I GTCTC 1 cut(s) 132
ApeKI GCWGC 1 cut(s) 58
AspLEI GCGC 1 cut(s) 58
BaeGI GKGCMC 1 cut(s) 273
BanI GGYRCC 1 cut(s) 268
BbvI GCAGC 1 cut(s) 45
BccI CCATC 2 cut(s) 37, 208
BciT130I CCWGG 1 cut(s) 258
BclI TGATCA 1 cut(s) 187
BcoDI GTCTC 1 cut(s) 132
BfaI CTAG 1 cut(s) 35
BfoI RGCGCY 1 cut(s) 59
BisI GCNGC 1 cut(s) 59
BlsI GCNGC 1 cut(s) 60
Bme1390I CCNGG 1 cut(s) 258
BmiI GGNNCC 1 cut(s) 270
BmrFI CCNGG 1 cut(s) 258
BmsI GCATC 1 cut(s) 243
BpmI CTGGAG 1 cut(s) 279
BsaI GGTCTC 1 cut(s) 132
BsaWI WCCGGW 1 cut(s) 307
BsaXI ACNNNNNCTCC 2 cut(s) 252, 282
Bsc4I CCNNNNNNNGG 1 cut(s) 112
BseBI CCWGG 1 cut(s) 258
BseLI CCNNNNNNNGG 1 cut(s) 112
BseSI GKGCMC 1 cut(s) 273
BseXI GCAGC 1 cut(s) 45
BsgI GTGCAG 1 cut(s) 356
BshNI GGYRCC 1 cut(s) 268
BsiSI CCGG 1 cut(s) 308
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 1 cut(s) 112
BsmAI GTCTC 1 cut(s) 132
BsmFI GGGAC 1 cut(s) 128
Bso31I GGTCTC 1 cut(s) 132
Bsp1286I GDGCHC 1 cut(s) 273
Bsp143I GATC 2 cut(s) 166, 187
BspLI GGNNCC 1 cut(s) 270
BspQI GCTCTTC 1 cut(s) 230
BspT107I GGYRCC 1 cut(s) 268
BspTNI GGTCTC 1 cut(s) 132
BssMI GATC 2 cut(s) 166, 187
Bst2UI CCWGG 1 cut(s) 258
Bst4CI ACNGT 1 cut(s) 247
Bst6I CTCTTC 1 cut(s) 230
BstH2I RGCGCY 1 cut(s) 59
BstHHI GCGC 1 cut(s) 58
BstKTI GATC 2 cut(s) 169, 190
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 2 cut(s) 166, 187
BstMWI GCNNNNNNNGC 2 cut(s) 67, 229
BstNI CCWGG 1 cut(s) 258
BstSCI CCNGG 1 cut(s) 256
BstSLI GKGCMC 1 cut(s) 273
BstV1I GCAGC 1 cut(s) 45
BtsIMutI CAGTG 1 cut(s) 252
CfoI GCGC 1 cut(s) 58
CseI GACGC 1 cut(s) 70
Csp6I GTAC 1 cut(s) 87
CviAII CATG 2 cut(s) 211, 297
CviJI RGCY 5 cut(s) 51, 240, 304, 322, 343
CviKI_1 RGCY 5 cut(s) 51, 240, 304, 322, 343
CviQI GTAC 1 cut(s) 87
DpnI GATC 2 cut(s) 168, 189
DpnII GATC 2 cut(s) 166, 187
Eam1104I CTCTTC 1 cut(s) 230
EarI CTCTTC 1 cut(s) 230
Eco31I GGTCTC 1 cut(s) 132
EcoRII CCWGG 1 cut(s) 256
FaeI CATG 2 cut(s) 214, 300
FaiI YATR 4 cut(s) 171, 212, 281, 298
FaqI GGGAC 1 cut(s) 128
FatI CATG 2 cut(s) 210, 296
FbaI TGATCA 1 cut(s) 187
Fnu4HI GCNGC 1 cut(s) 59
Fsp4HI GCNGC 1 cut(s) 59
FspBI CTAG 1 cut(s) 35
GlaI GCGC 1 cut(s) 57
GluI GCNGC 1 cut(s) 59
GsuI CTGGAG 1 cut(s) 279
HaeII RGCGCY 1 cut(s) 59
HapII CCGG 1 cut(s) 308
HgaI GACGC 1 cut(s) 70
HhaI GCGC 1 cut(s) 58
Hin1II CATG 2 cut(s) 214, 300
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HindIII AAGCTT 1 cut(s) 320
HpaII CCGG 1 cut(s) 308
Hpy188I TCNGA 1 cut(s) 192
Hpy188III TCNNGA 1 cut(s) 35
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 3 cut(s) 100, 116, 146
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4V TGCA 1 cut(s) 337
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 229
Hsp92II CATG 2 cut(s) 214, 300
HspAI GCGC 1 cut(s) 56
Ksp22I TGATCA 1 cut(s) 187
Kzo9I GATC 2 cut(s) 166, 187
LguI GCTCTTC 1 cut(s) 230
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 9 cut(s) 57, 174, 188, 192, 243, 251, 254, 270, 321
Lsp1109I GCAGC 1 cut(s) 45
LweI GCATC 1 cut(s) 243
MaeI CTAG 1 cut(s) 35
MalI GATC 2 cut(s) 168, 189
MboI GATC 2 cut(s) 166, 187
MboII GAAGA 3 cut(s) 85, 161, 247
MfeI CAATTG 1 cut(s) 285
MhlI GDGCHC 1 cut(s) 273
MluCI AATT 1 cut(s) 285
MnlI CCTC 2 cut(s) 151, 315
MspI CCGG 1 cut(s) 308
MspR9I CCNGG 1 cut(s) 258
MunI CAATTG 1 cut(s) 285
MvaI CCWGG 1 cut(s) 258
MwoI GCNNNNNNNGC 2 cut(s) 67, 229
NdeII GATC 2 cut(s) 166, 187
NlaIII CATG 2 cut(s) 214, 300
NlaIV GGNNCC 1 cut(s) 270
PciSI GCTCTTC 1 cut(s) 230
PkrI GCNGC 1 cut(s) 60
Psp6I CCWGG 1 cut(s) 256
PspGI CCWGG 1 cut(s) 256
PspN4I GGNNCC 1 cut(s) 270
RsaI GTAC 1 cut(s) 88
RsaNI GTAC 1 cut(s) 87
SapI GCTCTTC 1 cut(s) 230
SatI GCNGC 1 cut(s) 59
Sau3AI GATC 2 cut(s) 166, 187
ScrFI CCNGG 1 cut(s) 258
SduI GDGCHC 1 cut(s) 273
SetI ASST 5 cut(s) 92, 123, 199, 270, 324
SfaNI GCATC 1 cut(s) 243
Sse9I AATT 1 cut(s) 285
SspMI CTAG 1 cut(s) 35
StyD4I CCNGG 1 cut(s) 256
TaaI ACNGT 1 cut(s) 247
TaqII GACCGA 1 cut(s) 114
TasI AATT 1 cut(s) 285
TscAI CASTG 1 cut(s) 252
TseI GCWGC 1 cut(s) 58
TspDTI ATGAA 2 cut(s) 98, 268
TspRI CASTG 1 cut(s) 252
XbaI TCTAGA 1 cut(s) 34
XspI CTAG 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.