RLG00000017769

At5g65660-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004182
Physical Location & Seq
Forward (+)
20999617 .. 21000113
497 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017769

Sequence Viewer

Length: 405 bp
ATGGAGAGCCAGGATCTTTCGCCACCGCACGTGGATGCGTCTCGGCCGTCCTTGGGGTTCCCTCTCGGCACCGCGCTTCTCCTCATCATCATCTTCAGCTTGAGCGGTGTCTTCTCCTGCTGCTACCACTGGGACAAGCTCAGATCACTCCGCAGGTCTTTCTCCGAAGACTCCACTCCCGAAACCTCCGACGACATCGAAGGCCAATCTCCCTCCAAATCTAAACCCGCCCATCTGGACTTGAACCAGAATCAAAAGCAAAGCGTGCCGGTGATAATGCCCGGCGATCAGATTCCGAAGTTTATGGCATTGCCGTGTCCTCGTGAGCCTCCGAGAGAAGAGAAGATAGTTGTCAAAGTTCAAAAACCGCCCAAGCCACCTCGATTTCCTGTACCTCTGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

135

Amino Acids

14.99

Weight (kDa)

7.73

Isoelectric Point (pI)

76.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 144
AccB1I GGYRCC 1 cut(s) 68
AccBSI CCGCTC 1 cut(s) 105
AccII CGCG 1 cut(s) 74
AciI CCGC 6 cut(s) 26, 72, 105, 151, 228, 368
AclWI GGATC 1 cut(s) 21
AcoI YGGCCR 1 cut(s) 44
AcuI CTGAAG 1 cut(s) 79
AcvI CACGTG 1 cut(s) 31
AfaI GTAC 1 cut(s) 393
AfiI CCNNNNNNNGG 1 cut(s) 53
AgsI TTSAA 2 cut(s) 244, 362
AjnI CCWGG 1 cut(s) 9
AluBI AGCT 2 cut(s) 99, 139
AluI AGCT 2 cut(s) 99, 139
Alw26I GTCTC 1 cut(s) 45
AlwI GGATC 1 cut(s) 21
AoxI GGCC 2 cut(s) 44, 202
ApeKI GCWGC 1 cut(s) 120
AspLEI GCGC 1 cut(s) 76
AsuC2I CCSGG 1 cut(s) 282
AsuHPI GGTGA 1 cut(s) 283
BanI GGYRCC 1 cut(s) 68
BauI CACGAG 1 cut(s) 321
BbrPI CACGTG 1 cut(s) 31
BbsI GAAGAC 2 cut(s) 103, 174
BbvI GCAGC 1 cut(s) 107
BccI CCATC 1 cut(s) 240
BceAI ACGGC 2 cut(s) 31, 298
BciT130I CCWGG 1 cut(s) 11
BcnI CCSGG 1 cut(s) 282
BcoDI GTCTC 1 cut(s) 45
BfuAI ACCTGC 1 cut(s) 144
BisI GCNGC 1 cut(s) 121
BlsI GCNGC 1 cut(s) 122
Bme1390I CCNGG 2 cut(s) 11, 282
BmiI GGNNCC 2 cut(s) 59, 70
BmrFI CCNGG 2 cut(s) 11, 282
BmrI ACTGGG 1 cut(s) 139
BmsI GCATC 1 cut(s) 25
BmuI ACTGGG 1 cut(s) 139
BpiI GAAGAC 2 cut(s) 103, 174
BpuEI CTTGAG 1 cut(s) 121
BpuMI CCSGG 1 cut(s) 282
BsaAI YACGTR 1 cut(s) 31
BsaJI CCNNGG 1 cut(s) 51
Bsc4I CCNNNNNNNGG 1 cut(s) 53
Bse118I RCCGGY 1 cut(s) 268
Bse1I ACTGG 1 cut(s) 134
Bse3DI GCAATG 1 cut(s) 308
BseBI CCWGG 1 cut(s) 11
BseDI CCNNGG 1 cut(s) 51
BseGI GGATG 1 cut(s) 40
BseLI CCNNNNNNNGG 1 cut(s) 53
BseMI GCAATG 1 cut(s) 308
BseMII CTCAG 1 cut(s) 154
BseNI ACTGG 1 cut(s) 134
BseRI GAGGAG 1 cut(s) 71
BseX3I CGGCCG 1 cut(s) 44
BseXI GCAGC 1 cut(s) 107
Bsh1236I CGCG 1 cut(s) 74
Bsh1285I CGRYCG 1 cut(s) 47
BshFI GGCC 2 cut(s) 46, 204
BshNI GGYRCC 1 cut(s) 68
BsiEI CGRYCG 1 cut(s) 47
BsiSI CCGG 2 cut(s) 269, 282
BslFI GGGAC 1 cut(s) 146
BslI CCNNNNNNNGG 1 cut(s) 53
BsmAI GTCTC 1 cut(s) 45
BsmBI CGTCTC 1 cut(s) 45
BsmFI GGGAC 1 cut(s) 146
BsnI GGCC 2 cut(s) 46, 204
Bsp143I GATC 3 cut(s) 13, 143, 286
BspACI CCGC 6 cut(s) 26, 72, 105, 151, 228, 368
BspANI GGCC 2 cut(s) 46, 204
BspCNI CTCAG 1 cut(s) 153
BspFNI CGCG 1 cut(s) 74
BspLI GGNNCC 2 cut(s) 59, 70
BspMI ACCTGC 1 cut(s) 144
BspPI GGATC 1 cut(s) 21
BspT107I GGYRCC 1 cut(s) 68
BsrBI CCGCTC 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 308
BsrFI RCCGGY 1 cut(s) 268
BsrI ACTGG 1 cut(s) 134
BssAI RCCGGY 1 cut(s) 268
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 3 cut(s) 13, 143, 286
BssSI CACGAG 1 cut(s) 321
BssT1I CCWWGG 1 cut(s) 51
Bst2BI CACGAG 1 cut(s) 321
Bst2UI CCWGG 1 cut(s) 11
Bst6I CTCTTC 1 cut(s) 333
BstAPI GCANNNNNTGC 1 cut(s) 265
BstBAI YACGTR 1 cut(s) 31
BstC8I GCNNGC 1 cut(s) 266
BstDEI CTNAG 1 cut(s) 140
BstF5I GGATG 1 cut(s) 40
BstFNI CGCG 1 cut(s) 74
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 3 cut(s) 16, 146, 289
BstMAI GTCTC 1 cut(s) 45
BstMBI GATC 3 cut(s) 13, 143, 286
BstMCI CGRYCG 1 cut(s) 47
BstMWI GCNNNNNNNGC 1 cut(s) 265
BstNI CCWGG 1 cut(s) 11
BstSCI CCNGG 2 cut(s) 9, 280
BstUI CGCG 1 cut(s) 74
BstV1I GCAGC 1 cut(s) 107
BstV2I GAAGAC 2 cut(s) 103, 174
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
BstZI CGGCCG 1 cut(s) 44
BsuRI GGCC 2 cut(s) 46, 204
BtsCI GGATG 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 127
BveI ACCTGC 1 cut(s) 144
Cac8I GCNNGC 1 cut(s) 266
CfoI GCGC 1 cut(s) 76
Cfr10I RCCGGY 1 cut(s) 268
CseI GACGC 1 cut(s) 27
Csp6I GTAC 1 cut(s) 392
CviJI RGCY 7 cut(s) 9, 46, 99, 139, 204, 328, 376
CviKI_1 RGCY 7 cut(s) 9, 46, 99, 139, 204, 328, 376
CviQI GTAC 1 cut(s) 392
DdeI CTNAG 1 cut(s) 140
DpnI GATC 3 cut(s) 15, 145, 288
DpnII GATC 3 cut(s) 13, 143, 286
EaeI YGGCCR 1 cut(s) 44
EagI CGGCCG 1 cut(s) 44
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
EclXI CGGCCG 1 cut(s) 44
Eco130I CCWWGG 1 cut(s) 51
Eco52I CGGCCG 1 cut(s) 44
Eco57I CTGAAG 1 cut(s) 79
Eco72I CACGTG 1 cut(s) 31
EcoRII CCWGG 1 cut(s) 9
EcoT14I CCWWGG 1 cut(s) 51
ErhI CCWWGG 1 cut(s) 51
Esp3I CGTCTC 1 cut(s) 45
FaiI YATR 1 cut(s) 305
FaqI GGGAC 1 cut(s) 146
FauI CCCGC 1 cut(s) 235
Fnu4HI GCNGC 1 cut(s) 121
FokI GGATG 1 cut(s) 47
Fsp4HI GCNGC 1 cut(s) 121
GlaI GCGC 1 cut(s) 75
GluI GCNGC 1 cut(s) 121
HaeIII GGCC 2 cut(s) 46, 204
HapII CCGG 2 cut(s) 269, 282
HgaI GACGC 1 cut(s) 27
HhaI GCGC 1 cut(s) 76
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HinfI GANTC 3 cut(s) 170, 250, 292
HpaII CCGG 2 cut(s) 269, 282
HphI GGTGA 1 cut(s) 283
Hpy188I TCNGA 6 cut(s) 143, 166, 190, 291, 297, 333
Hpy188III TCNNGA 3 cut(s) 179, 236, 323
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 1 cut(s) 194
HpyCH4IV ACGT 1 cut(s) 30
HpyF10VI GCNNNNNNNGC 1 cut(s) 265
HpyF3I CTNAG 1 cut(s) 140
HpySE526I ACGT 1 cut(s) 30
HspAI GCGC 1 cut(s) 74
Kzo9I GATC 3 cut(s) 13, 143, 286
LpnPI CCDG 8 cut(s) 23, 115, 130, 139, 221, 260, 282, 295
Lsp1109I GCAGC 1 cut(s) 107
LweI GCATC 1 cut(s) 25
MaeII ACGT 1 cut(s) 30
MalI GATC 3 cut(s) 15, 145, 288
MbiI CCGCTC 1 cut(s) 105
MboI GATC 3 cut(s) 13, 143, 286
MboII GAAGA 5 cut(s) 85, 103, 179, 350, 355
MflI RGATCY 1 cut(s) 13
MlyI GAGTC 1 cut(s) 164
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 8 cut(s) 72, 92, 196, 223, 330, 339, 390, 405
MslI CAYNNNNRTG 2 cut(s) 33, 313
MspI CCGG 2 cut(s) 269, 282
MspR9I CCNGG 2 cut(s) 11, 282
MvaI CCWGG 1 cut(s) 11
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 1 cut(s) 265
NciI CCSGG 1 cut(s) 282
NdeII GATC 3 cut(s) 13, 143, 286
NlaIV GGNNCC 2 cut(s) 59, 70
NmeAIII GCCGAG 2 cut(s) 22, 45
PfeI GAWTC 2 cut(s) 250, 292
PkrI GCNGC 1 cut(s) 122
PleI GAGTC 1 cut(s) 164
PmaCI CACGTG 1 cut(s) 31
PmlI CACGTG 1 cut(s) 31
PpsI GAGTC 1 cut(s) 164
Ppu21I YACGTR 1 cut(s) 31
Psp6I CCWGG 1 cut(s) 9
PspCI CACGTG 1 cut(s) 31
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 2 cut(s) 59, 70
PsuI RGATCY 1 cut(s) 13
RsaI GTAC 1 cut(s) 393
RsaNI GTAC 1 cut(s) 392
RseI CAYNNNNRTG 2 cut(s) 33, 313
SatI GCNGC 1 cut(s) 121
Sau3AI GATC 3 cut(s) 13, 143, 286
SchI GAGTC 1 cut(s) 164
ScrFI CCNGG 2 cut(s) 11, 282
SetI ASST 7 cut(s) 33, 101, 141, 158, 188, 382, 397
SfaNI GCATC 1 cut(s) 25
SmiMI CAYNNNNRTG 2 cut(s) 33, 313
SmlI CTYRAG 1 cut(s) 100
SmoI CTYRAG 1 cut(s) 100
SsiI CCGC 6 cut(s) 26, 72, 105, 151, 228, 368
StyD4I CCNGG 2 cut(s) 9, 280
StyI CCWWGG 1 cut(s) 51
TaiI ACGT 1 cut(s) 33
TaqI TCGA 2 cut(s) 198, 382
TfiI GAWTC 2 cut(s) 250, 292
TscAI CASTG 1 cut(s) 134
TseI GCWGC 1 cut(s) 120
TspRI CASTG 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.