Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0003231

Physical Location & Seq

Genomic Coordinates Reverse (-)

17814505 .. 17815848

Gene Structure 1344 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1073000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1344 bp

ATGGAGGTGGAGAAGAAGTATATTTCTGTAGAGGAGTTGAAGAAACACAACAAGCCTGGAGATTTATGGATCTCTATCCAGGGCAAGGTCTACAATGTCTCCGATTGGACGAAAGATCACCCCGGCGGCGATACCATTCTGTACAATCTGGGTGGCCTGGATGTTACAGACGCGTTCATCGCTTACCATCCCCGCACTGCATGGCAGTACCTCAAGAAATTCTCCACCGGAATTTATCTCAAAGATTTCGAGGTCTCCGAAGTGTCGAAAGATTACAGAAGAATGGCAACGGAGTTTTCGAAGGCGGGTTTGTTCGAGAAGAAGGGGCACGGCGCTCTGTGCTCGATCACCATCATTTCAATCATGTTCTGCCTCTCCGTGTACGGAGTTTTGAAGTCCGACAGCGTTTTGGTGCATTTGATGAGCGGGATGCTTCTGGGGATGTTGTGGATAGAGGGAGCTTACGTCGGACATGATTGCGGGCATTACCAGGTAATGCCAAGCCAACTGTGCAACAACATTGCCCAGATTGTTGCCGGTAATTGCCTTACGGGAATCAGCATCGCCTGGTGGAAGTGGACTCATAATGCCCACCACATTGCCTGCAACAGCCTCGATTACGACCCGGATCTCCAGCACATTCCGGTTTTCGCCGTCTCCACTAAGTTTTTCAATTCGATCACCTCGATTTTTTACGGGAGAGTTTTGACATTTGATCCTCTGGCTAGGTTTTTGGTCAGCTACCAGCACTTTACATATTATCCGGTTATGATGGTGGGGAGGGTTAATTTGTTCATCCAGACATTCTTGCTCTTGTGTTCGAGGCGGGATGTCCCAAACAGAGGATTGAACATCATGGGAATCCTAGTTTTCTGGACATGGTTTCCTCTCCTCGTTTCGTGTTTGCCCAACTGGAGTGAGAGGGTTATGTTTGTGCTGGCAGCATTTGCAGTGACATCCCTCCAGCACATCCAATTCACATTGAATCATTTCTCCGGCGACACCTATCTCGGTGCCCCCAATGGCAATGACTGGTTCGAGAAGCAAGCCGCGGGGACTGTCGATATCTCCTGCTCAACTAAGATGGATTGGTTCTTCGGCGGGCTGCAGTTCCAGCTCGAACATCATTTGTTTCCGCGAATGCCTCGCGGACAACTGAGGAAGATTGCTCCTACTGTGAAGGAGTTGTGCAAGAAGCACAATTTGCCATACAGGAGCTTGTCCTTCTGGGAGGCCAATAAGGCCACACTCAGGACTCTAAGGACTGCCGCCCTCCAGGCTAGAGACCTGTCGAATCCAGTCCCGAAGAACTTGGTCTGGGAAGCTTTGAACACTCATGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006629 GO:0006643 GO:0006665 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009266 GO:0009409 GO:0009628 GO:0009987 GO:0012505 GO:0016491 GO:0016705 GO:0030148 GO:0033554 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046467 GO:0050896 GO:0051716 GO:0052631 GO:0055114 GO:0070417 GO:0071704 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cyt-b5 FA_desaturase

Protein Analysis

448

Amino Acids

50.96

Weight (kDa)

8.77

Isoelectric Point (pI)

36.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cyt-b5	PF00173	10 - 77	3e-22	Cytochrome b5-like Heme/Steroid binding domain
FA_desaturase	PF00487	138 - 407	6.6e-34	Fatty acid desaturase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003231)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46210 AT3G61580
fragaria_vesca	FvH4_7g15550 FvH4_7g15550 FvH4_7g15570
malus_domestica	MD01G1072900.v1.1 MD01G1073000.v1.1 MD07G1129700.v1.1 MD07G1130000.v1.1
prunus_persica	Prupe.2G179200_v2.0.a1 Prupe.2G179200_v2.0.a1 Prupe.2G179400_v2.0.a1
pyrus_communis	pycom01g10190 pycom01g10200 pycom07g12680 pycom07g12700
rosa_chinensis	RchiOBHm_Chr1g0358201 RchiOBHm_Chr1g0358241
rosa_laevigata	RLG00000027980 RLG00000027983
rosa_multiflora	Rmu_sc0002071.1_g000009 Rmu_sc0007072.1_g000001 Rmu_sc0023145.1_g000004
rosa_roxburghii	Rroxscaffold_4G00298340 Rroxscaffold_4G00298370
rosa_rugosa	Rorug01G0259800 Rorug01G0259900
rosa_samantha	Rh1AG273500 Rh1AG273900 Rh1BG240700 Rh1BG241000 Rh1CG257100 Rh1CG257500 Rh1DG269600 Rh1DG269700
rosa_wichuraiana	Rw1G024450 Rw1G024480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1013
AccBSI	CCGCTC	1 cut(s)	426
AccI	GTMKAC	1 cut(s)	90
AccII	CGCG	4 cut(s)	173, 1052, 1138, 1149
AclWI	GGATC	3 cut(s)	77, 636, 710
AcsI	RAATTY	2 cut(s)	218, 231
AfaI	GTAC	3 cut(s)	143, 209, 383
AfiI	CCNNNNNNNGG	3 cut(s)	85, 842, 1251
AflIII	ACRYGT	1 cut(s)	171
AgsI	TTSAA	7 cut(s)	40, 360, 394, 673, 850, 985, 1330
AjnI	CCWGG	6 cut(s)	55, 78, 156, 489, 566, 1275
AluBI	AGCT	5 cut(s)	461, 741, 1117, 1218, 1325
AluI	AGCT	5 cut(s)	461, 741, 1117, 1218, 1325
Alw21I	GWGCWC	1 cut(s)	344
Alw26I	GTCTC	4 cut(s)	103, 259, 661, 1278
AlwI	GGATC	3 cut(s)	77, 636, 710
AoxI	GGCC	3 cut(s)	154, 1233, 1242
ApeKI	GCWGC	2 cut(s)	941, 1105
ApoI	RAATTY	2 cut(s)	218, 231
Asp700I	GAANNNNTTC	1 cut(s)	989
AspLEI	GCGC	1 cut(s)	335
AsuC2I	CCSGG	2 cut(s)	123, 626
AsuHPI	GGTGA	3 cut(s)	110, 340, 673
AsuII	TTCGAA	1 cut(s)	299
BaeGI	GKGCMC	2 cut(s)	330, 1018
BanI	GGYRCC	1 cut(s)	1013
Bbv12I	GWGCWC	1 cut(s)	344
BbvI	GCAGC	2 cut(s)	953, 1092
BccI	CCATC	4 cut(s)	195, 359, 766, 1078
BceAI	ACGGC	2 cut(s)	346, 638
BcgI	CGANNNNNNTGC	2 cut(s)	595, 629
BciT130I	CCWGG	6 cut(s)	57, 80, 158, 491, 568, 1277
BcnI	CCSGG	2 cut(s)	123, 626
BcoDI	GTCTC	4 cut(s)	103, 259, 661, 1278
BfaI	CTAG	3 cut(s)	726, 866, 1281
BfmI	CTRYAG	2 cut(s)	27, 1106
BfoI	RGCGCY	1 cut(s)	336
BglI	GCCNNNNNGGC	2 cut(s)	1241, 1277
BisI	GCNGC	5 cut(s)	127, 942, 1050, 1106, 1269
BlsI	GCNGC	5 cut(s)	128, 943, 1051, 1107, 1270
Bme1390I	CCNGG	8 cut(s)	57, 80, 123, 158, 491, 568, 626, 1277
BmiI	GGNNCC	1 cut(s)	1015
BmrFI	CCNGG	8 cut(s)	57, 80, 123, 158, 491, 568, 626, 1277
BmsI	GCATC	2 cut(s)	420, 570
BpmI	CTGGAG	5 cut(s)	78, 617, 934, 947, 1259
Bpu14I	TTCGAA	1 cut(s)	299
BpuEI	CTTGAG	1 cut(s)	197
BpuMI	CCSGG	2 cut(s)	123, 626
BsaBI	GATNNNNATC	2 cut(s)	74, 350
BsaI	GGTCTC	2 cut(s)	259, 1278
BsaJI	CCNNGG	3 cut(s)	79, 121, 1050
BsaWI	WCCGGW	3 cut(s)	227, 643, 763
BsaXI	ACNNNNNCTCC	5 cut(s)	32, 83, 113, 450, 480
Bsc4I	CCNNNNNNNGG	3 cut(s)	85, 842, 1251
Bse118I	RCCGGY	1 cut(s)	536
Bse1I	ACTGG	3 cut(s)	917, 1037, 1298
Bse3DI	GCAATG	3 cut(s)	519, 597, 1033
Bse8I	GATNNNNATC	2 cut(s)	74, 350
BseBI	CCWGG	6 cut(s)	57, 80, 158, 491, 568, 1277
BseDI	CCNNGG	3 cut(s)	79, 121, 1050
BseGI	GGATG	8 cut(s)	166, 187, 435, 447, 795, 835, 956, 969
BseJI	GATNNNNATC	2 cut(s)	74, 350
BseLI	CCNNNNNNNGG	3 cut(s)	85, 842, 1251
BseMI	GCAATG	3 cut(s)	519, 597, 1033
BseMII	CTCAG	2 cut(s)	1148, 1264
BseNI	ACTGG	3 cut(s)	917, 1037, 1298
BseRI	GAGGAG	2 cut(s)	47, 881
BseSI	GKGCMC	2 cut(s)	330, 1018
BseXI	GCAGC	2 cut(s)	953, 1092
Bsh1236I	CGCG	4 cut(s)	173, 1052, 1138, 1149
BshFI	GGCC	3 cut(s)	156, 1235, 1244
BshNI	GGYRCC	1 cut(s)	1013
BsiHKAI	GWGCWC	1 cut(s)	344
BsiSI	CCGG	7 cut(s)	123, 228, 537, 626, 644, 764, 996
BslFI	GGGAC	3 cut(s)	818, 1069, 1286
BslI	CCNNNNNNNGG	3 cut(s)	85, 842, 1251
BsmAI	GTCTC	4 cut(s)	103, 259, 661, 1278
BsmBI	CGTCTC	1 cut(s)	661
BsmFI	GGGAC	3 cut(s)	818, 1069, 1286
BsmI	GAATGC	1 cut(s)	1146
BsnI	GGCC	3 cut(s)	156, 1235, 1244
Bso31I	GGTCTC	2 cut(s)	259, 1278
Bsp119I	TTCGAA	1 cut(s)	299
Bsp1286I	GDGCHC	3 cut(s)	330, 344, 1018
Bsp1407I	TGTACA	1 cut(s)	141
Bsp143I	GATC	6 cut(s)	69, 115, 345, 628, 678, 715
BspANI	GGCC	3 cut(s)	156, 1235, 1244
BspCNI	CTCAG	2 cut(s)	1149, 1263
BspFNI	CGCG	4 cut(s)	173, 1052, 1138, 1149
BspLI	GGNNCC	1 cut(s)	1015
BspMAI	CTGCAG	1 cut(s)	1110
BspPI	GGATC	3 cut(s)	77, 636, 710
BspT104I	TTCGAA	1 cut(s)	299
BspT107I	GGYRCC	1 cut(s)	1013
BspTNI	GGTCTC	2 cut(s)	259, 1278
BsrBI	CCGCTC	1 cut(s)	426
BsrDI	GCAATG	3 cut(s)	519, 597, 1033
BsrFI	RCCGGY	1 cut(s)	536
BsrGI	TGTACA	1 cut(s)	141
BsrI	ACTGG	3 cut(s)	917, 1037, 1298
BssAI	RCCGGY	1 cut(s)	536
BssECI	CCNNGG	3 cut(s)	79, 121, 1050
BssMI	GATC	6 cut(s)	69, 115, 345, 628, 678, 715
Bst2UI	CCWGG	6 cut(s)	57, 80, 158, 491, 568, 1277
Bst4CI	ACNGT	3 cut(s)	510, 1060, 1177
BstAPI	GCANNNNNTGC	2 cut(s)	947, 1204
BstAUI	TGTACA	1 cut(s)	141
BstBI	TTCGAA	1 cut(s)	299
BstC8I	GCNNGC	5 cut(s)	482, 604, 939, 1047, 1103
BstDEI	CTNAG	5 cut(s)	663, 1080, 1157, 1250, 1259
BstDSI	CCRYGG	1 cut(s)	1050
BstF5I	GGATG	8 cut(s)	166, 187, 435, 447, 795, 835, 956, 969
BstFNI	CGCG	4 cut(s)	173, 1052, 1138, 1149
BstH2I	RGCGCY	1 cut(s)	336
BstHHI	GCGC	1 cut(s)	335
BstKTI	GATC	6 cut(s)	72, 118, 348, 631, 681, 718
BstMAI	GTCTC	4 cut(s)	103, 259, 661, 1278
BstMBI	GATC	6 cut(s)	69, 115, 345, 628, 678, 715
BstMWI	GCNNNNNNNGC	8 cut(s)	179, 339, 510, 947, 1114, 1204, 1241, 1277
BstNI	CCWGG	6 cut(s)	57, 80, 158, 491, 568, 1277
BstSCI	CCNGG	8 cut(s)	55, 78, 121, 156, 489, 566, 624, 1275
BstSFI	CTRYAG	2 cut(s)	27, 1106
BstSLI	GKGCMC	2 cut(s)	330, 1018
BstUI	CGCG	4 cut(s)	173, 1052, 1138, 1149
BstV1I	GCAGC	2 cut(s)	953, 1092
BstX2I	RGATCY	2 cut(s)	69, 628
BstYI	RGATCY	2 cut(s)	69, 628
BsuRI	GGCC	3 cut(s)	156, 1235, 1244
BtgI	CCRYGG	1 cut(s)	1050
BtgZI	GCGATG	2 cut(s)	163, 547
BtsCI	GGATG	8 cut(s)	166, 187, 435, 447, 795, 835, 956, 969
BtsI	GCAGTG	2 cut(s)	195, 957
BtsIMutI	CAGTG	2 cut(s)	195, 957
Cac8I	GCNNGC	5 cut(s)	482, 604, 939, 1047, 1103
CfoI	GCGC	1 cut(s)	335
Cfr10I	RCCGGY	1 cut(s)	536
Cfr42I	CCGCGG	1 cut(s)	1053
CseI	GACGC	1 cut(s)	179
CsiI	ACCWGGT	1 cut(s)	489
Csp6I	GTAC	3 cut(s)	142, 208, 382
CspCI	CAANNNNNGTGG	2 cut(s)	133, 168
CviAII	CATG	6 cut(s)	201, 364, 473, 856, 879, 1337
CviQI	GTAC	3 cut(s)	142, 208, 382
DdeI	CTNAG	5 cut(s)	663, 1080, 1157, 1250, 1259
DpnI	GATC	6 cut(s)	71, 117, 347, 630, 680, 717
DpnII	GATC	6 cut(s)	69, 115, 345, 628, 678, 715
Eco31I	GGTCTC	2 cut(s)	259, 1278
Eco32I	GATATC	1 cut(s)	1066
EcoRII	CCWGG	6 cut(s)	55, 78, 156, 489, 566, 1275
EcoRV	GATATC	1 cut(s)	1066
Esp3I	CGTCTC	1 cut(s)	661
FaeI	CATG	6 cut(s)	204, 367, 476, 859, 882, 1340
FaqI	GGGAC	3 cut(s)	818, 1069, 1286
FatI	CATG	6 cut(s)	200, 363, 472, 855, 878, 1336
FauI	CCCGC	7 cut(s)	200, 298, 419, 473, 819, 1045, 1094
FblI	GTMKAC	1 cut(s)	90
Fnu4HI	GCNGC	5 cut(s)	127, 942, 1050, 1106, 1269
FokI	GGATG	8 cut(s)	173, 174, 442, 454, 782, 842, 943, 956
Fsp4HI	GCNGC	5 cut(s)	127, 942, 1050, 1106, 1269
FspBI	CTAG	3 cut(s)	726, 866, 1281
GlaI	GCGC	1 cut(s)	334
GluI	GCNGC	5 cut(s)	127, 942, 1050, 1106, 1269
GsuI	CTGGAG	5 cut(s)	78, 617, 934, 947, 1259
HaeII	RGCGCY	1 cut(s)	336
HaeIII	GGCC	3 cut(s)	156, 1235, 1244
HapII	CCGG	7 cut(s)	123, 228, 537, 626, 644, 764, 996
HgaI	GACGC	1 cut(s)	179
HhaI	GCGC	1 cut(s)	335
Hin1II	CATG	6 cut(s)	204, 367, 476, 859, 882, 1340
Hin6I	GCGC	1 cut(s)	333
HinP1I	GCGC	1 cut(s)	333
HindIII	AAGCTT	1 cut(s)	1323
HinfI	GANTC	6 cut(s)	555, 580, 861, 985, 1255, 1294
HpaII	CCGG	7 cut(s)	123, 228, 537, 626, 644, 764, 996
HphI	GGTGA	3 cut(s)	110, 340, 673
Hpy166II	GTNNAC	3 cut(s)	91, 382, 579
Hpy188I	TCNGA	4 cut(s)	103, 259, 400, 470
Hpy188III	TCNNGA	7 cut(s)	214, 316, 799, 874, 1039, 1252, 1303
Hpy8I	GTNNAC	3 cut(s)	91, 382, 579
Hpy99I	CGWCG	1 cut(s)	470
HpyAV	CCTTC	4 cut(s)	295, 316, 1174, 1234
HpyCH4III	ACNGT	3 cut(s)	510, 1060, 1177
HpyCH4IV	ACGT	1 cut(s)	465
HpyCH4V	TGCA	7 cut(s)	200, 415, 513, 606, 950, 1108, 1191
HpyF10VI	GCNNNNNNNGC	8 cut(s)	179, 339, 510, 947, 1114, 1204, 1241, 1277
HpyF3I	CTNAG	5 cut(s)	663, 1080, 1157, 1250, 1259
HpySE526I	ACGT	1 cut(s)	465
Hsp92II	CATG	6 cut(s)	204, 367, 476, 859, 882, 1340
HspAI	GCGC	1 cut(s)	333
KspI	CCGCGG	1 cut(s)	1053
Kzo9I	GATC	6 cut(s)	69, 115, 345, 628, 678, 715
LmnI	GCTCC	3 cut(s)	458, 1174, 1215
Lsp1109I	GCAGC	2 cut(s)	953, 1092
LweI	GCATC	2 cut(s)	420, 570
MabI	ACCWGGT	1 cut(s)	489
MaeI	CTAG	3 cut(s)	726, 866, 1281
MaeII	ACGT	1 cut(s)	465
MaeIII	GTNAC	2 cut(s)	163, 952
MalI	GATC	6 cut(s)	71, 117, 347, 630, 680, 717
MbiI	CCGCTC	1 cut(s)	426
MboI	GATC	6 cut(s)	69, 115, 345, 628, 678, 715
MboII	GAAGA	7 cut(s)	25, 52, 291, 331, 1087, 1174, 1318
MflI	RGATCY	2 cut(s)	69, 628
MhlI	GDGCHC	3 cut(s)	330, 344, 1018
MluCI	AATT	7 cut(s)	218, 231, 541, 673, 787, 974, 1201
MluI	ACGCGT	1 cut(s)	171
MlyI	GAGTC	2 cut(s)	574, 1249
MmeI	TCCRAC	2 cut(s)	423, 448
MroXI	GAANNNNTTC	1 cut(s)	989
MseI	TTAA	1 cut(s)	786
MspA1I	CMGCKG	1 cut(s)	1052
MspI	CCGG	7 cut(s)	123, 228, 537, 626, 644, 764, 996
MspR9I	CCNGG	8 cut(s)	57, 80, 123, 158, 491, 568, 626, 1277
Mva1269I	GAATGC	1 cut(s)	1146
MvaI	CCWGG	6 cut(s)	57, 80, 158, 491, 568, 1277
MvnI	CGCG	4 cut(s)	173, 1052, 1138, 1149
MwoI	GCNNNNNNNGC	8 cut(s)	179, 339, 510, 947, 1114, 1204, 1241, 1277
NciI	CCSGG	2 cut(s)	123, 626
NdeII	GATC	6 cut(s)	69, 115, 345, 628, 678, 715
NlaIII	CATG	6 cut(s)	204, 367, 476, 859, 882, 1340
NlaIV	GGNNCC	1 cut(s)	1015
NmuCI	GTSAC	1 cut(s)	952
NspV	TTCGAA	1 cut(s)	299
PcsI	WCGNNNNNNNCGW	3 cut(s)	255, 296, 683
PctI	GAATGC	1 cut(s)	1146
PdmI	GAANNNNTTC	1 cut(s)	989
PfeI	GAWTC	4 cut(s)	555, 861, 985, 1294
PkrI	GCNGC	5 cut(s)	128, 943, 1051, 1107, 1270
PleI	GAGTC	2 cut(s)	574, 1249
PpsI	GAGTC	2 cut(s)	574, 1249
Psp6I	CCWGG	6 cut(s)	55, 78, 156, 489, 566, 1275
PspGI	CCWGG	6 cut(s)	55, 78, 156, 489, 566, 1275
PspN4I	GGNNCC	1 cut(s)	1015
PstI	CTGCAG	1 cut(s)	1110
PsuI	RGATCY	2 cut(s)	69, 628
RsaI	GTAC	3 cut(s)	143, 209, 383
RsaNI	GTAC	3 cut(s)	142, 208, 382
SacII	CCGCGG	1 cut(s)	1053
SaqAI	TTAA	1 cut(s)	786
SatI	GCNGC	5 cut(s)	127, 942, 1050, 1106, 1269
Sau3AI	GATC	6 cut(s)	69, 115, 345, 628, 678, 715
SchI	GAGTC	2 cut(s)	574, 1249
ScrFI	CCNGG	8 cut(s)	57, 80, 123, 158, 491, 568, 626, 1277
SduI	GDGCHC	3 cut(s)	330, 344, 1018
SexAI	ACCWGGT	1 cut(s)	489
SfaNI	GCATC	2 cut(s)	420, 570
SfcI	CTRYAG	2 cut(s)	27, 1106
SfiI	GGCCNNNNNGGCC	1 cut(s)	1241
Sfr303I	CCGCGG	1 cut(s)	1053
SfuI	TTCGAA	1 cut(s)	299
SgrBI	CCGCGG	1 cut(s)	1053
SmlI	CTYRAG	1 cut(s)	212
SmoI	CTYRAG	1 cut(s)	212
Sse9I	AATT	7 cut(s)	218, 231, 541, 673, 787, 974, 1201
SspMI	CTAG	3 cut(s)	726, 866, 1281
StyD4I	CCNGG	8 cut(s)	55, 78, 121, 156, 489, 566, 624, 1275
TaaI	ACNGT	3 cut(s)	510, 1060, 1177
TaiI	ACGT	1 cut(s)	468
TasI	AATT	7 cut(s)	218, 231, 541, 673, 787, 974, 1201
TatI	WGTACW	1 cut(s)	141
TauI	GCSGC	3 cut(s)	129, 1052, 1271
TfiI	GAWTC	4 cut(s)	555, 861, 985, 1294
Tru1I	TTAA	1 cut(s)	786
Tru9I	TTAA	1 cut(s)	786
TscAI	CASTG	2 cut(s)	202, 957
TseFI	GTSAC	1 cut(s)	952
TseI	GCWGC	2 cut(s)	941, 1105
Tsp45I	GTSAC	1 cut(s)	952
TspDTI	ATGAA	2 cut(s)	166, 784
TspGWI	ACGGA	3 cut(s)	305, 367, 399
TspRI	CASTG	2 cut(s)	202, 957
XapI	RAATTY	2 cut(s)	218, 231
XmiI	GTMKAC	1 cut(s)	90
XmnI	GAANNNNTTC	1 cut(s)	989
XspI	CTAG	3 cut(s)	726, 866, 1281

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.