Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0003231

Physical Location & Seq

Genomic Coordinates Forward (+)

13996731 .. 13998080

Gene Structure 1350 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom07g12680.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1350 bp

ATGGAAATGGAGGTGGAGAAGAAGTATATTTCTGCAGAGGAGCTGAAGAAGCACAAGAAGCCTAGAGATTTATGGATCTCTATCCAGGGCAAGGTTTACAATGTGTCCGATTGGGCGAAAGATCACCCCGGCGGCGATACGATTCTGTACAATCTGGGCGGCCAGGATGTTACAGACGCCTTCATCGCCTACCATCCCGGCACTGCATGGCAGTACCTCAAGAAATTCTCCACCGGAATTTATCTCAAAGATTTCGAGGTCTCCGAGGTGTCGAAAGATTACAGAAGAATGGCCACGGAGTTTTCGAAGGCGGGTTTGTTCGAGAAGAAGGGACACGGCGCTCTGTGCTCAATCGTTTTCATTGCCATCATGCTCAACCTCGTCGTGTACGGAGTTTTGAAATCCGACAGCGTCTTGGTACATTTGATCTGCGGGATGCTTCTGGGGATGTTGTGGATTGAGGGAGCTTACATCGGACACGATTCGGGGCATTACCAGGTAATGTCGAGCCGATTTTACAACAACATTGCCCAGATTGTTGCCGGTAATTGCTTCACCGGAATCAGCATTGCCTGGTGGAAGTGGACGCACAATGCCCACCACATTGCCTGCAACAGCCTCGATTACGACCCGGATCTCCAGCACATTCCGGTTTTTGCTGTCTCCGCAAAGTTTTTCAATTCGATCACCTCGATTTTTTACGGGAGAGTTTTGACGTTTGATCCTCTGGCAAGGCTTTTGGTCAGCTACCAGCATTTTACATATTACCCGGTTATGATGGTGGGGAGGGTTAATTTGTTTATCCAGACATTCTTGCTCTTGTTTTCGAGGCGGAATGTCCCGAACAGGGGATTGAACATCATCGGAATCCTTGTTTTCTGGACATGGTTTCCCCTCCTCGTTTCGTGTCTGCCCAATTGGAGTGAGAGGGTTATGTTTGTGCTGGCAGCATTTGCAGTGACGTCGCTCCAGCACATCCAATTCACGTTGAATCATTTCTCCGGCGACACCTATCTCGGTGCCCCCAATGCCAATGACTGGTTCGAGAAGCAAGCCGCGGGGACTATCGATATCTCCTGCTCAACTTGGATGGATTGGTTCTTTGGCGGCCTGCAGTTCCAGCTCGAACATCATTTGTTTCCGCGAATGCCTCGCGGACAACTGAGGAAGATTGCTCCAACTGTGAAGGAGCTGTGCAAGAAGCACAATTTGCCATACAGGAGCTTGTCCTTCTGGGAGGCCAATGTGTCCACAATTAGGACTCTAAGGAATGCTGCCCTTCAGGCTAGGGACCTGTCCAATCAAGTCCCGAAGAACTTGGTCTGGGAAGCTTTGAACACTCATGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006629 GO:0006643 GO:0006665 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009266 GO:0009409 GO:0009628 GO:0009987 GO:0012505 GO:0016491 GO:0016705 GO:0030148 GO:0033554 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046467 GO:0050896 GO:0051716 GO:0052631 GO:0055114 GO:0070417 GO:0071704 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cyt-b5 FA_desaturase

Protein Analysis

450

Amino Acids

51.42

Weight (kDa)

8.88

Isoelectric Point (pI)

34.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cyt-b5	PF00173	12 - 79	7.2e-23	Cytochrome b5-like Heme/Steroid binding domain
FA_desaturase	PF00487	139 - 409	7.1e-35	Fatty acid desaturase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003231)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46210 AT3G61580
fragaria_vesca	FvH4_7g15550 FvH4_7g15550 FvH4_7g15570
malus_domestica	MD01G1072900.v1.1 MD01G1073000.v1.1 MD07G1129700.v1.1 MD07G1130000.v1.1
prunus_persica	Prupe.2G179200_v2.0.a1 Prupe.2G179200_v2.0.a1 Prupe.2G179400_v2.0.a1
pyrus_communis	pycom01g10190 pycom01g10200 pycom07g12680 pycom07g12700
rosa_chinensis	RchiOBHm_Chr1g0358201 RchiOBHm_Chr1g0358241
rosa_laevigata	RLG00000027980 RLG00000027983
rosa_multiflora	Rmu_sc0002071.1_g000009 Rmu_sc0007072.1_g000001 Rmu_sc0023145.1_g000004
rosa_roxburghii	Rroxscaffold_4G00298340 Rroxscaffold_4G00298370
rosa_rugosa	Rorug01G0259800 Rorug01G0259900
rosa_samantha	Rh1AG273500 Rh1AG273900 Rh1BG240700 Rh1BG241000 Rh1CG257100 Rh1CG257500 Rh1DG269600 Rh1DG269700
rosa_wichuraiana	Rw1G024450 Rw1G024480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	965
AccB1I	GGYRCC	1 cut(s)	1019
AccB7I	CCANNNNNTGG	1 cut(s)	1038
AccII	CGCG	3 cut(s)	1058, 1144, 1155
AclWI	GGATC	3 cut(s)	83, 642, 716
AcoI	YGGCCR	2 cut(s)	160, 291
AcsI	RAATTY	2 cut(s)	224, 237
AcuI	CTGAAG	2 cut(s)	65, 1265
AcyI	GRCGYC	2 cut(s)	177, 962
AfaI	GTAC	4 cut(s)	149, 215, 389, 420
AfiI	CCNNNNNNNGG	5 cut(s)	91, 847, 848, 1038, 1257
AgsI	TTSAA	5 cut(s)	400, 679, 856, 991, 1336
AjnI	CCWGG	4 cut(s)	84, 162, 495, 572
AjuI	GAANNNNNNNTTGG	2 cut(s)	1025, 1057
AluBI	AGCT	7 cut(s)	43, 467, 747, 1123, 1192, 1224, 1331
AluI	AGCT	7 cut(s)	43, 467, 747, 1123, 1192, 1224, 1331
Alw21I	GWGCWC	1 cut(s)	350
Alw26I	GTCTC	2 cut(s)	265, 667
AlwI	GGATC	3 cut(s)	83, 642, 716
AoxI	GGCC	4 cut(s)	160, 291, 1108, 1239
ApeKI	GCWGC	2 cut(s)	947, 1274
ApoI	RAATTY	2 cut(s)	224, 237
Asp700I	GAANNNNTTC	1 cut(s)	995
AspLEI	GCGC	1 cut(s)	341
AspS9I	GGNCC	1 cut(s)	1291
AsuC2I	CCSGG	4 cut(s)	129, 198, 632, 770
AsuHPI	GGTGA	3 cut(s)	116, 547, 679
AsuII	TTCGAA	1 cut(s)	305
AvaII	GGWCC	1 cut(s)	1291
BaeGI	GKGCMC	1 cut(s)	1024
BalI	TGGCCA	1 cut(s)	293
BanI	GGYRCC	1 cut(s)	1019
Bbv12I	GWGCWC	1 cut(s)	350
BbvI	GCAGC	2 cut(s)	959, 1261
BccI	CCATC	4 cut(s)	201, 374, 772, 1084
BceAI	ACGGC	1 cut(s)	352
BcgI	CGANNNNNNTGC	4 cut(s)	601, 635, 945, 979
BciT130I	CCWGG	4 cut(s)	86, 164, 497, 574
BcnI	CCSGG	4 cut(s)	129, 198, 632, 770
BcoDI	GTCTC	2 cut(s)	265, 667
BfaI	CTAG	2 cut(s)	63, 1287
BfmI	CTRYAG	2 cut(s)	33, 1112
BfoI	RGCGCY	1 cut(s)	342
BglI	GCCNNNNNGGC	1 cut(s)	1283
BisI	GCNGC	6 cut(s)	133, 160, 948, 1056, 1108, 1275
BlsI	GCNGC	6 cut(s)	134, 161, 949, 1057, 1109, 1276
Bme1390I	CCNGG	8 cut(s)	86, 129, 164, 198, 497, 574, 632, 770
Bme18I	GGWCC	1 cut(s)	1291
BmgT120I	GGNCC	1 cut(s)	1291
BmiI	GGNNCC	2 cut(s)	1021, 1292
BmrFI	CCNGG	8 cut(s)	86, 129, 164, 198, 497, 574, 632, 770
BmsI	GCATC	1 cut(s)	426
BpmI	CTGGAG	2 cut(s)	623, 953
Bpu14I	TTCGAA	1 cut(s)	305
BpuEI	CTTGAG	1 cut(s)	203
BpuMI	CCSGG	4 cut(s)	129, 198, 632, 770
Bsa29I	ATCGAT	1 cut(s)	1068
BsaBI	GATNNNNATC	1 cut(s)	80
BsaHI	GRCGYC	2 cut(s)	177, 962
BsaI	GGTCTC	1 cut(s)	265
BsaJI	CCNNGG	5 cut(s)	85, 127, 264, 294, 1056
BsaWI	WCCGGW	3 cut(s)	233, 557, 649
BsaXI	ACNNNNNCTCC	2 cut(s)	8, 38
Bsc4I	CCNNNNNNNGG	5 cut(s)	91, 847, 848, 1038, 1257
Bse118I	RCCGGY	1 cut(s)	542
Bse1I	ACTGG	1 cut(s)	1043
Bse3DI	GCAATG	4 cut(s)	360, 525, 567, 603
Bse8I	GATNNNNATC	1 cut(s)	80
BseBI	CCWGG	4 cut(s)	86, 164, 497, 574
BseCI	ATCGAT	1 cut(s)	1068
BseDI	CCNNGG	5 cut(s)	85, 127, 264, 294, 1056
BseGI	GGATG	6 cut(s)	172, 193, 441, 453, 975, 1095
BseJI	GATNNNNATC	1 cut(s)	80
BseLI	CCNNNNNNNGG	5 cut(s)	91, 847, 848, 1038, 1257
BseMI	GCAATG	4 cut(s)	360, 525, 567, 603
BseMII	CTCAG	1 cut(s)	1154
BseNI	ACTGG	1 cut(s)	1043
BseRI	GAGGAG	2 cut(s)	53, 887
BseSI	GKGCMC	1 cut(s)	1024
BseXI	GCAGC	2 cut(s)	959, 1261
Bsh1236I	CGCG	3 cut(s)	1058, 1144, 1155
BshFI	GGCC	4 cut(s)	162, 293, 1110, 1241
BshNI	GGYRCC	1 cut(s)	1019
BshVI	ATCGAT	1 cut(s)	1068
BsiHKAI	GWGCWC	1 cut(s)	350
BsiSI	CCGG	9 cut(s)	129, 198, 234, 543, 558, 632, 650, 770, 1002
BslFI	GGGAC	5 cut(s)	345, 824, 1075, 1292, 1304
BslI	CCNNNNNNNGG	5 cut(s)	91, 847, 848, 1038, 1257
BsmAI	GTCTC	2 cut(s)	265, 667
BsmFI	GGGAC	5 cut(s)	345, 824, 1075, 1292, 1304
BsmI	GAATGC	2 cut(s)	1152, 1276
BsnI	GGCC	4 cut(s)	162, 293, 1110, 1241
Bso31I	GGTCTC	1 cut(s)	265
Bsp119I	TTCGAA	1 cut(s)	305
Bsp1286I	GDGCHC	2 cut(s)	350, 1024
Bsp1407I	TGTACA	1 cut(s)	147
Bsp143I	GATC	6 cut(s)	75, 121, 426, 634, 684, 721
BspANI	GGCC	4 cut(s)	162, 293, 1110, 1241
BspCNI	CTCAG	1 cut(s)	1155
BspDI	ATCGAT	1 cut(s)	1068
BspFNI	CGCG	3 cut(s)	1058, 1144, 1155
BspLI	GGNNCC	2 cut(s)	1021, 1292
BspMAI	CTGCAG	2 cut(s)	37, 1116
BspPI	GGATC	3 cut(s)	83, 642, 716
BspT104I	TTCGAA	1 cut(s)	305
BspT107I	GGYRCC	1 cut(s)	1019
BspTNI	GGTCTC	1 cut(s)	265
BsrDI	GCAATG	4 cut(s)	360, 525, 567, 603
BsrFI	RCCGGY	1 cut(s)	542
BsrGI	TGTACA	1 cut(s)	147
BsrI	ACTGG	1 cut(s)	1043
BssAI	RCCGGY	1 cut(s)	542
BssECI	CCNNGG	5 cut(s)	85, 127, 264, 294, 1056
BssMI	GATC	6 cut(s)	75, 121, 426, 634, 684, 721
BssNI	GRCGYC	2 cut(s)	177, 962
Bst2UI	CCWGG	4 cut(s)	86, 164, 497, 574
Bst4CI	ACNGT	1 cut(s)	1183
BstACI	GRCGYC	2 cut(s)	177, 962
BstAPI	GCANNNNNTGC	2 cut(s)	953, 1210
BstAUI	TGTACA	1 cut(s)	147
BstBI	TTCGAA	1 cut(s)	305
BstC8I	GCNNGC	4 cut(s)	610, 945, 1053, 1112
BstDEI	CTNAG	2 cut(s)	1163, 1265
BstDSI	CCRYGG	2 cut(s)	294, 1056
BstF5I	GGATG	6 cut(s)	172, 193, 441, 453, 975, 1095
BstFNI	CGCG	3 cut(s)	1058, 1144, 1155
BstH2I	RGCGCY	1 cut(s)	342
BstHHI	GCGC	1 cut(s)	341
BstKTI	GATC	6 cut(s)	78, 124, 429, 637, 687, 724
BstMAI	GTCTC	2 cut(s)	265, 667
BstMBI	GATC	6 cut(s)	75, 121, 426, 634, 684, 721
BstNI	CCWGG	4 cut(s)	86, 164, 497, 574
BstSCI	CCNGG	8 cut(s)	84, 127, 162, 196, 495, 572, 630, 768
BstSFI	CTRYAG	2 cut(s)	33, 1112
BstSLI	GKGCMC	1 cut(s)	1024
BstUI	CGCG	3 cut(s)	1058, 1144, 1155
BstV1I	GCAGC	2 cut(s)	959, 1261
BstX2I	RGATCY	2 cut(s)	75, 634
BstYI	RGATCY	2 cut(s)	75, 634
Bsu15I	ATCGAT	1 cut(s)	1068
BsuRI	GGCC	4 cut(s)	162, 293, 1110, 1241
BsuTUI	ATCGAT	1 cut(s)	1068
BtgI	CCRYGG	2 cut(s)	294, 1056
BtgZI	GCGATG	1 cut(s)	169
BtsCI	GGATG	6 cut(s)	172, 193, 441, 453, 975, 1095
BtsI	GCAGTG	2 cut(s)	201, 963
BtsIMutI	CAGTG	2 cut(s)	201, 963
Cac8I	GCNNGC	4 cut(s)	610, 945, 1053, 1112
CfoI	GCGC	1 cut(s)	341
Cfr10I	RCCGGY	1 cut(s)	542
Cfr13I	GGNCC	1 cut(s)	1291
Cfr42I	CCGCGG	1 cut(s)	1059
ClaI	ATCGAT	1 cut(s)	1068
CseI	GACGC	3 cut(s)	185, 400, 595
CsiI	ACCWGGT	1 cut(s)	495
Csp6I	GTAC	4 cut(s)	148, 214, 388, 419
CviAII	CATG	4 cut(s)	207, 370, 885, 1343
CviQI	GTAC	4 cut(s)	148, 214, 388, 419
DdeI	CTNAG	2 cut(s)	1163, 1265
DpnI	GATC	6 cut(s)	77, 123, 428, 636, 686, 723
DpnII	GATC	6 cut(s)	75, 121, 426, 634, 684, 721
EaeI	YGGCCR	2 cut(s)	160, 291
EciI	GGCGGA	1 cut(s)	847
Eco31I	GGTCTC	1 cut(s)	265
Eco32I	GATATC	1 cut(s)	1072
Eco47I	GGWCC	1 cut(s)	1291
Eco57I	CTGAAG	2 cut(s)	65, 1265
EcoO109I	RGGNCCY	1 cut(s)	1291
EcoRII	CCWGG	4 cut(s)	84, 162, 495, 572
EcoRV	GATATC	1 cut(s)	1072
FaeI	CATG	4 cut(s)	210, 373, 888, 1346
FaqI	GGGAC	5 cut(s)	345, 824, 1075, 1292, 1304
FatI	CATG	4 cut(s)	206, 369, 884, 1342
FauI	CCCGC	3 cut(s)	304, 425, 1051
Fnu4HI	GCNGC	6 cut(s)	133, 160, 948, 1056, 1108, 1275
FokI	GGATG	6 cut(s)	179, 180, 448, 460, 962, 1102
Fsp4HI	GCNGC	6 cut(s)	133, 160, 948, 1056, 1108, 1275
FspBI	CTAG	2 cut(s)	63, 1287
GlaI	GCGC	1 cut(s)	340
GluI	GCNGC	6 cut(s)	133, 160, 948, 1056, 1108, 1275
GsuI	CTGGAG	2 cut(s)	623, 953
HaeII	RGCGCY	1 cut(s)	342
HaeIII	GGCC	4 cut(s)	162, 293, 1110, 1241
HapII	CCGG	9 cut(s)	129, 198, 234, 543, 558, 632, 650, 770, 1002
HgaI	GACGC	3 cut(s)	185, 400, 595
HhaI	GCGC	1 cut(s)	341
Hin1I	GRCGYC	2 cut(s)	177, 962
Hin1II	CATG	4 cut(s)	210, 373, 888, 1346
Hin6I	GCGC	1 cut(s)	339
HinP1I	GCGC	1 cut(s)	339
HindIII	AAGCTT	1 cut(s)	1329
HinfI	GANTC	6 cut(s)	142, 482, 561, 867, 991, 1261
HpaII	CCGG	9 cut(s)	129, 198, 234, 543, 558, 632, 650, 770, 1002
HphI	GGTGA	3 cut(s)	116, 547, 679
Hpy166II	GTNNAC	4 cut(s)	97, 388, 585, 1251
Hpy188I	TCNGA	5 cut(s)	109, 265, 406, 476, 866
Hpy188III	TCNNGA	7 cut(s)	220, 322, 805, 841, 880, 1045, 1309
Hpy8I	GTNNAC	4 cut(s)	97, 388, 585, 1251
Hpy99I	CGWCG	2 cut(s)	386, 967
HpyAV	CCTTC	6 cut(s)	190, 301, 322, 1180, 1240, 1289
HpyCH4III	ACNGT	1 cut(s)	1183
HpyCH4IV	ACGT	3 cut(s)	716, 962, 986
HpyCH4V	TGCA	6 cut(s)	35, 206, 612, 956, 1114, 1197
HpyF3I	CTNAG	2 cut(s)	1163, 1265
HpySE526I	ACGT	3 cut(s)	716, 962, 986
Hsp92I	GRCGYC	2 cut(s)	177, 962
Hsp92II	CATG	4 cut(s)	210, 373, 888, 1346
HspAI	GCGC	1 cut(s)	339
KspI	CCGCGG	1 cut(s)	1059
Kzo9I	GATC	6 cut(s)	75, 121, 426, 634, 684, 721
LmnI	GCTCC	6 cut(s)	40, 464, 972, 1180, 1189, 1221
Lsp1109I	GCAGC	2 cut(s)	959, 1261
LweI	GCATC	1 cut(s)	426
MabI	ACCWGGT	1 cut(s)	495
MaeI	CTAG	2 cut(s)	63, 1287
MaeII	ACGT	3 cut(s)	716, 962, 986
MaeIII	GTNAC	2 cut(s)	169, 958
MalI	GATC	6 cut(s)	77, 123, 428, 636, 686, 723
MboI	GATC	6 cut(s)	75, 121, 426, 634, 684, 721
MboII	GAAGA	6 cut(s)	31, 58, 297, 337, 1180, 1324
MfeI	CAATTG	1 cut(s)	916
MflI	RGATCY	2 cut(s)	75, 634
MhlI	GDGCHC	2 cut(s)	350, 1024
MlsI	TGGCCA	1 cut(s)	293
MluCI	AATT	9 cut(s)	224, 237, 547, 679, 793, 916, 980, 1207, 1254
MluNI	TGGCCA	1 cut(s)	293
MlyI	GAGTC	1 cut(s)	1255
MmeI	TCCRAC	2 cut(s)	429, 1202
Mox20I	TGGCCA	1 cut(s)	293
MroXI	GAANNNNTTC	1 cut(s)	995
MscI	TGGCCA	1 cut(s)	293
MseI	TTAA	1 cut(s)	792
Msp20I	TGGCCA	1 cut(s)	293
MspA1I	CMGCKG	1 cut(s)	1058
MspI	CCGG	9 cut(s)	129, 198, 234, 543, 558, 632, 650, 770, 1002
MspR9I	CCNGG	8 cut(s)	86, 129, 164, 198, 497, 574, 632, 770
MunI	CAATTG	1 cut(s)	916
Mva1269I	GAATGC	2 cut(s)	1152, 1276
MvaI	CCWGG	4 cut(s)	86, 164, 497, 574
MvnI	CGCG	3 cut(s)	1058, 1144, 1155
NciI	CCSGG	4 cut(s)	129, 198, 632, 770
NdeII	GATC	6 cut(s)	75, 121, 426, 634, 684, 721
NlaIII	CATG	4 cut(s)	210, 373, 888, 1346
NlaIV	GGNNCC	2 cut(s)	1021, 1292
NmuCI	GTSAC	1 cut(s)	958
NspV	TTCGAA	1 cut(s)	305
PcsI	WCGNNNNNNNCGW	3 cut(s)	261, 302, 689
PctI	GAATGC	2 cut(s)	1152, 1276
PdmI	GAANNNNTTC	1 cut(s)	995
PfeI	GAWTC	5 cut(s)	142, 482, 561, 867, 991
PflFI	GACNNNGTC	1 cut(s)	410
PflMI	CCANNNNNTGG	1 cut(s)	1038
PkrI	GCNGC	6 cut(s)	134, 161, 949, 1057, 1109, 1276
PleI	GAGTC	1 cut(s)	1255
PpsI	GAGTC	1 cut(s)	1255
PpuMI	RGGWCCY	1 cut(s)	1291
Psp5II	RGGWCCY	1 cut(s)	1291
Psp6I	CCWGG	4 cut(s)	84, 162, 495, 572
PspGI	CCWGG	4 cut(s)	84, 162, 495, 572
PspN4I	GGNNCC	2 cut(s)	1021, 1292
PspPI	GGNCC	1 cut(s)	1291
PspPPI	RGGWCCY	1 cut(s)	1291
PstI	CTGCAG	2 cut(s)	37, 1116
PsuI	RGATCY	2 cut(s)	75, 634
PsyI	GACNNNGTC	1 cut(s)	410
RsaI	GTAC	4 cut(s)	149, 215, 389, 420
RsaNI	GTAC	4 cut(s)	148, 214, 388, 419
SacII	CCGCGG	1 cut(s)	1059
SaqAI	TTAA	1 cut(s)	792
SatI	GCNGC	6 cut(s)	133, 160, 948, 1056, 1108, 1275
Sau3AI	GATC	6 cut(s)	75, 121, 426, 634, 684, 721
Sau96I	GGNCC	1 cut(s)	1291
SchI	GAGTC	1 cut(s)	1255
ScrFI	CCNGG	8 cut(s)	86, 129, 164, 198, 497, 574, 632, 770
SduI	GDGCHC	2 cut(s)	350, 1024
SexAI	ACCWGGT	1 cut(s)	495
SfaNI	GCATC	1 cut(s)	426
SfcI	CTRYAG	2 cut(s)	33, 1112
Sfr303I	CCGCGG	1 cut(s)	1059
SfuI	TTCGAA	1 cut(s)	305
SgrBI	CCGCGG	1 cut(s)	1059
SinI	GGWCC	1 cut(s)	1291
SmlI	CTYRAG	1 cut(s)	218
SmoI	CTYRAG	1 cut(s)	218
Sse9I	AATT	9 cut(s)	224, 237, 547, 679, 793, 916, 980, 1207, 1254
SspMI	CTAG	2 cut(s)	63, 1287
StyD4I	CCNGG	8 cut(s)	84, 127, 162, 196, 495, 572, 630, 768
TaaI	ACNGT	1 cut(s)	1183
TaiI	ACGT	3 cut(s)	719, 965, 989
TasI	AATT	9 cut(s)	224, 237, 547, 679, 793, 916, 980, 1207, 1254
TatI	WGTACW	1 cut(s)	147
TauI	GCSGC	4 cut(s)	135, 162, 1058, 1110
TfiI	GAWTC	5 cut(s)	142, 482, 561, 867, 991
Tru1I	TTAA	1 cut(s)	792
Tru9I	TTAA	1 cut(s)	792
TscAI	CASTG	2 cut(s)	208, 963
TseFI	GTSAC	1 cut(s)	958
TseI	GCWGC	2 cut(s)	947, 1274
Tsp45I	GTSAC	1 cut(s)	958
TspDTI	ATGAA	2 cut(s)	172, 349
TspGWI	ACGGA	2 cut(s)	311, 405
TspRI	CASTG	2 cut(s)	208, 963
Tth111I	GACNNNGTC	1 cut(s)	410
Van91I	CCANNNNNTGG	1 cut(s)	1038
VpaK11BI	GGWCC	1 cut(s)	1291
XapI	RAATTY	2 cut(s)	224, 237
XmnI	GAANNNNTTC	1 cut(s)	995
XspI	CTAG	2 cut(s)	63, 1287
ZraI	GACGTC	1 cut(s)	963

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.