Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0003231

Physical Location & Seq

Genomic Coordinates Forward (+)

17493702 .. 17495910

Gene Structure 2209 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000027983

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1503 bp

ATGGCTACATTCCATTCCCCCCTCCCCAAAATATCTTTGCAGCCAAGTCTCCCTTTGGGGCAGTTTGAGTCTTTACTGAATTTGAAAGTTGGAGAACAGAGTTTGACGTTTAGGCTTTGGTTTTTTGACTCAAAGGTTTGGTTCATAATGGAGGAGAAAATGGAGGTTGAGAAGAAGTATATTACTGTTGAGGAACTGAAGCTGCACAACAAGCCTGGGGACCTATGGATCTCTATCCAGGGCAAGATTTACAATGTCTCAGATTGGGTTCATGATCACCCAGGTGGAGCTACCATTTTGTATGATTTGGGTGGCCAAGATGTTACTGATGGATTCATGGCATACCATCCTAGCACTGTGTGGAAGCGCCTAGGGAAGTACTTCAATGGGTATCATCTCAAGGATTTCGAGATCTCAGAGGCGTCCAAGGACTATAGGAAGCTTCATGCTGAGATTACGCAGATGGGTTTGTTCGAAAACAAAGGGCATGGGGCTCTTTACAATATTACAACTATTGTTGTTTTGCTTAACCTTGTTGTGTATGGTGTGTTGAAGTCTGATAGTGTGTTGGTCCATTTTGTTTGTGCTGTGATGTTGGGGGTCCTGTGGATTATGGGTGCCTATATGGGTCATGATTCTGGTCATTACCAAGTCATGTCAAGCCGTAAGTGGAACCAAATTATTCAGGTTCTGGCTGGGAATTGCCTTACTGGGATTAGCATTGCTTGGTGGAAATGGACTCACAACGCACATCATATTGCCTGCAACAGCCTGGATTATGATCCTGATCTTCAACACATGCCGGTCTTTGCAGTGTCCACAAAGTTCTTTCATTCAATTACTTCAGTCTTTTATGGGAGGAAGTTGGATTTTGATCATGTGGCTAGGTTTTTAGTCAGCCATCAGCATTTTACTTATTATCCTATCATGATTGTTGGGAGGGTCAACTTGTTTATTCAGACCTTCTTGTTGTTGTTGTCCAAGAGAAGCATCCCCAATAGGACTATGAACATAATGGGAATCCTTGTTTTCTGGACTTGGTTTCCTCTGCTTGTCATGTGTCTGCCCAGTTGGAGTGAGAGGGTGATGTTCGTGCTTGTGAGCTTTGCAGTGACAGCACTCCAGCACATTCAGTTCACTTTGAACCATTTCTCAGGAGATACTTATCTTGGACCACCAACTGGGAATGATTGGTTTGAGAAGCAGACTGCTGGAACCATTGATATTTCGTGCTCAAAGTACATGGATTGGTTCTTTGGTGGTCTGCAGTTTCAGCTAGAGCACCATTTGTTCCCCCGATTACCCCGATGCCAACTGAGGACGGTTTCTCCAACCGTGAAGGAACTATGCAAGAAGCACAATTTGCCTCACAGAAGCTTGTCCTTCTGGGATGCCAATGTGACCACCATTAGGGTTCTCAGGAAAGCTGCCCTGCAGGCTTGGGACATGACCCTTCCTGTCCCGAAGAACTTGGCTTGGGAAGCTATTAATACCCATGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006629 GO:0006643 GO:0006665 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009266 GO:0009409 GO:0009628 GO:0009987 GO:0012505 GO:0016491 GO:0016705 GO:0030148 GO:0033554 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046467 GO:0050896 GO:0051716 GO:0052631 GO:0055114 GO:0070417 GO:0071704 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cyt-b5 FA_desaturase

Protein Analysis

501

Amino Acids

57.65

Weight (kDa)

8.65

Isoelectric Point (pI)

40.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cyt-b5	PF00173	62 - 131	7.3e-21	Cytochrome b5-like Heme/Steroid binding domain
FA_desaturase	PF00487	190 - 459	3.5e-32	Fatty acid desaturase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003231)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46210 AT3G61580
fragaria_vesca	FvH4_7g15550 FvH4_7g15550 FvH4_7g15570
malus_domestica	MD01G1072900.v1.1 MD01G1073000.v1.1 MD07G1129700.v1.1 MD07G1130000.v1.1
prunus_persica	Prupe.2G179200_v2.0.a1 Prupe.2G179200_v2.0.a1 Prupe.2G179400_v2.0.a1
pyrus_communis	pycom01g10190 pycom01g10200 pycom07g12680 pycom07g12700
rosa_chinensis	RchiOBHm_Chr1g0358201 RchiOBHm_Chr1g0358241
rosa_laevigata	RLG00000027980 RLG00000027983
rosa_multiflora	Rmu_sc0002071.1_g000009 Rmu_sc0007072.1_g000001 Rmu_sc0023145.1_g000004
rosa_roxburghii	Rroxscaffold_4G00298340 Rroxscaffold_4G00298370
rosa_rugosa	Rorug01G0259800 Rorug01G0259900
rosa_samantha	Rh1AG273500 Rh1AG273900 Rh1BG240700 Rh1BG241000 Rh1CG257100 Rh1CG257500 Rh1DG269600 Rh1DG269700
rosa_wichuraiana	Rw1G024450 Rw1G024480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	617
AccB7I	CCANNNNNTGG	1 cut(s)	1181
AclWI	GGATC	2 cut(s)	236, 776
AcoI	YGGCCR	1 cut(s)	313
AcsI	RAATTY	1 cut(s)	79
AcuI	CTGAAG	2 cut(s)	218, 828
AcyI	GRCGYC	1 cut(s)	422
AdeI	CACNNNGTG	1 cut(s)	360
AfaI	GTAC	2 cut(s)	380, 1241
AfiI	CCNNNNNNNGG	2 cut(s)	1181, 1410
AgsI	TTSAA	6 cut(s)	85, 385, 553, 794, 837, 1144
AjnI	CCWGG	4 cut(s)	214, 237, 280, 771
AleI	CACNNNNGTG	1 cut(s)	282
AluBI	AGCT	8 cut(s)	202, 290, 442, 1104, 1276, 1377, 1427, 1484
AluI	AGCT	8 cut(s)	202, 290, 442, 1104, 1276, 1377, 1427, 1484
Alw21I	GWGCWC	2 cut(s)	1235, 1284
Alw26I	GTCTC	2 cut(s)	53, 262
AlwI	GGATC	2 cut(s)	236, 776
AlwNI	CAGNNNCTG	1 cut(s)	691
AoxI	GGCC	1 cut(s)	313
ApeKI	GCWGC	3 cut(s)	40, 202, 1427
ApoI	RAATTY	1 cut(s)	79
AseI	ATTAAT	1 cut(s)	1488
Asp700I	GAANNNNTTC	2 cut(s)	380, 1148
AspA2I	CCTAGG	1 cut(s)	370
AspLEI	GCGC	1 cut(s)	369
AspS9I	GGNCC	4 cut(s)	220, 571, 601, 1172
AsuHPI	GGTGA	2 cut(s)	269, 1096
AsuII	TTCGAA	1 cut(s)	474
AvaII	GGWCC	4 cut(s)	220, 571, 601, 1172
AvrII	CCTAGG	1 cut(s)	370
BalI	TGGCCA	1 cut(s)	315
BanI	GGYRCC	1 cut(s)	617
BanII	GRGCYC	1 cut(s)	496
Bbv12I	GWGCWC	2 cut(s)	1235, 1284
BbvI	GCAGC	3 cut(s)	52, 189, 1414
BccI	CCATC	4 cut(s)	323, 354, 457, 909
BceAI	ACGGC	1 cut(s)	648
BciT130I	CCWGG	4 cut(s)	216, 239, 282, 773
BclI	TGATCA	2 cut(s)	274, 874
BcoDI	GTCTC	2 cut(s)	53, 262
BfaI	CTAG	4 cut(s)	351, 371, 885, 1277
BfmI	CTRYAG	3 cut(s)	433, 1265, 1433
BfoI	RGCGCY	1 cut(s)	370
BglI	GCCNNNNNGGC	1 cut(s)	1436
BglII	AGATCT	1 cut(s)	411
BisI	GCNGC	3 cut(s)	41, 203, 1428
BlnI	CCTAGG	1 cut(s)	370
BlsI	GCNGC	3 cut(s)	42, 204, 1429
BmcAI	AGTACT	1 cut(s)	380
Bme1390I	CCNGG	4 cut(s)	216, 239, 282, 773
Bme18I	GGWCC	4 cut(s)	220, 571, 601, 1172
BmgT120I	GGNCC	4 cut(s)	220, 571, 601, 1172
BmiI	GGNNCC	5 cut(s)	221, 602, 619, 674, 1216
BmrFI	CCNGG	4 cut(s)	216, 239, 282, 773
BmrI	ACTGGG	3 cut(s)	720, 1062, 1191
BmsI	GCATC	3 cut(s)	999, 1298, 1381
BmuI	ACTGGG	3 cut(s)	720, 1062, 1191
BpmI	CTGGAG	1 cut(s)	1106
Bpu14I	TTCGAA	1 cut(s)	474
BpuEI	CTTGAG	1 cut(s)	383
BsaBI	GATNNNNATC	5 cut(s)	233, 780, 786, 873, 1164
BsaHI	GRCGYC	1 cut(s)	422
BsaJI	CCNNGG	6 cut(s)	215, 238, 280, 370, 426, 1495
BsaXI	ACNNNNNCTCC	2 cut(s)	1312, 1342
Bsc4I	CCNNNNNNNGG	2 cut(s)	1181, 1410
Bse118I	RCCGGY	1 cut(s)	802
Bse1I	ACTGG	3 cut(s)	715, 1068, 1186
Bse3DI	GCAATG	1 cut(s)	720
Bse8I	GATNNNNATC	5 cut(s)	233, 780, 786, 873, 1164
BseBI	CCWGG	4 cut(s)	216, 239, 282, 773
BseDI	CCNNGG	6 cut(s)	215, 238, 280, 370, 426, 1495
BseGI	GGATG	3 cut(s)	346, 990, 1396
BseJI	GATNNNNATC	5 cut(s)	233, 780, 786, 873, 1164
BseLI	CCNNNNNNNGG	2 cut(s)	1181, 1410
BseMI	GCAATG	1 cut(s)	720
BseMII	CTCAG	6 cut(s)	273, 429, 441, 1167, 1307, 1432
BseNI	ACTGG	3 cut(s)	715, 1068, 1186
BseRI	GAGGAG	1 cut(s)	167
BseXI	GCAGC	3 cut(s)	52, 189, 1414
BseYI	CCCAGC	1 cut(s)	695
BsgI	GTGCAG	1 cut(s)	188
BshFI	GGCC	1 cut(s)	315
BshNI	GGYRCC	1 cut(s)	617
BsiHKAI	GWGCWC	2 cut(s)	1235, 1284
BsiSI	CCGG	1 cut(s)	803
BslFI	GGGAC	3 cut(s)	233, 1445, 1457
BslI	CCNNNNNNNGG	2 cut(s)	1181, 1410
BsmAI	GTCTC	2 cut(s)	53, 262
BsmFI	GGGAC	3 cut(s)	233, 1445, 1457
BsnI	GGCC	1 cut(s)	315
Bsp119I	TTCGAA	1 cut(s)	474
Bsp1286I	GDGCHC	3 cut(s)	496, 1235, 1284
Bsp143I	GATC	6 cut(s)	228, 274, 411, 781, 787, 874
Bsp19I	CCATGG	1 cut(s)	1495
BspANI	GGCC	1 cut(s)	315
BspCNI	CTCAG	6 cut(s)	272, 428, 442, 1166, 1308, 1431
BspHI	TCATGA	3 cut(s)	271, 631, 927
BspLI	GGNNCC	5 cut(s)	221, 602, 619, 674, 1216
BspMAI	CTGCAG	2 cut(s)	1269, 1437
BspPI	GGATC	2 cut(s)	236, 776
BspT104I	TTCGAA	1 cut(s)	474
BspT107I	GGYRCC	1 cut(s)	617
BsrDI	GCAATG	1 cut(s)	720
BsrFI	RCCGGY	1 cut(s)	802
BsrI	ACTGG	3 cut(s)	715, 1068, 1186
BssAI	RCCGGY	1 cut(s)	802
BssECI	CCNNGG	6 cut(s)	215, 238, 280, 370, 426, 1495
BssMI	GATC	6 cut(s)	228, 274, 411, 781, 787, 874
BssNI	GRCGYC	1 cut(s)	422
BssT1I	CCWWGG	3 cut(s)	370, 426, 1495
Bst2UI	CCWGG	4 cut(s)	216, 239, 282, 773
Bst4CI	ACNGT	4 cut(s)	187, 358, 1324, 1336
BstACI	GRCGYC	1 cut(s)	422
BstAPI	GCANNNNNTGC	1 cut(s)	1363
BstBI	TTCGAA	1 cut(s)	474
BstC8I	GCNNGC	2 cut(s)	763, 1437
BstDEI	CTNAG	6 cut(s)	259, 415, 450, 1153, 1316, 1418
BstDSI	CCRYGG	1 cut(s)	1495
BstF5I	GGATG	3 cut(s)	346, 990, 1396
BstH2I	RGCGCY	1 cut(s)	370
BstHHI	GCGC	1 cut(s)	369
BstKTI	GATC	6 cut(s)	231, 277, 414, 784, 790, 877
BstMAI	GTCTC	2 cut(s)	53, 262
BstMBI	GATC	6 cut(s)	228, 274, 411, 781, 787, 874
BstMWI	GCNNNNNNNGC	6 cut(s)	211, 1115, 1273, 1363, 1436, 1481
BstNI	CCWGG	4 cut(s)	216, 239, 282, 773
BstNSI	RCATGY	1 cut(s)	802
BstSCI	CCNGG	4 cut(s)	214, 237, 280, 771
BstSFI	CTRYAG	3 cut(s)	433, 1265, 1433
BstV1I	GCAGC	3 cut(s)	52, 189, 1414
BstX2I	RGATCY	2 cut(s)	228, 411
BstYI	RGATCY	2 cut(s)	228, 411
BsuRI	GGCC	1 cut(s)	315
BtgI	CCRYGG	1 cut(s)	1495
BtsCI	GGATG	3 cut(s)	346, 990, 1396
BtsI	GCAGTG	2 cut(s)	819, 1116
BtsIMutI	CAGTG	3 cut(s)	354, 819, 1116
Cac8I	GCNNGC	2 cut(s)	763, 1437
CaiI	CAGNNNCTG	1 cut(s)	691
CciI	TCATGA	3 cut(s)	271, 631, 927
CfoI	GCGC	1 cut(s)	369
Cfr10I	RCCGGY	1 cut(s)	802
Cfr13I	GGNCC	4 cut(s)	220, 571, 601, 1172
CseI	GACGC	1 cut(s)	411
Csp6I	GTAC	2 cut(s)	379, 1240
CviQI	GTAC	2 cut(s)	379, 1240
DdeI	CTNAG	6 cut(s)	259, 415, 450, 1153, 1316, 1418
DpnI	GATC	6 cut(s)	230, 276, 413, 783, 789, 876
DpnII	GATC	6 cut(s)	228, 274, 411, 781, 787, 874
DraIII	CACNNNGTG	1 cut(s)	360
EaeI	YGGCCR	1 cut(s)	313
Eco130I	CCWWGG	3 cut(s)	370, 426, 1495
Eco24I	GRGCYC	1 cut(s)	496
Eco47I	GGWCC	4 cut(s)	220, 571, 601, 1172
Eco57I	CTGAAG	2 cut(s)	218, 828
EcoO109I	RGGNCCY	2 cut(s)	220, 601
EcoRII	CCWGG	4 cut(s)	214, 237, 280, 771
EcoT14I	CCWWGG	3 cut(s)	370, 426, 1495
EcoT38I	GRGCYC	1 cut(s)	496
ErhI	CCWWGG	3 cut(s)	370, 426, 1495
FalI	AAGNNNNNCTT	2 cut(s)	37, 69
FaqI	GGGAC	3 cut(s)	233, 1445, 1457
FbaI	TGATCA	2 cut(s)	274, 874
Fnu4HI	GCNGC	3 cut(s)	41, 203, 1428
FokI	GGATG	3 cut(s)	333, 977, 1403
FriOI	GRGCYC	1 cut(s)	496
Fsp4HI	GCNGC	3 cut(s)	41, 203, 1428
FspBI	CTAG	4 cut(s)	351, 371, 885, 1277
GlaI	GCGC	1 cut(s)	368
GluI	GCNGC	3 cut(s)	41, 203, 1428
GsaI	CCCAGC	1 cut(s)	699
GsuI	CTGGAG	1 cut(s)	1106
HaeII	RGCGCY	1 cut(s)	370
HaeIII	GGCC	1 cut(s)	315
HapII	CCGG	1 cut(s)	803
HgaI	GACGC	1 cut(s)	411
HhaI	GCGC	1 cut(s)	369
Hin1I	GRCGYC	1 cut(s)	422
Hin6I	GCGC	1 cut(s)	367
HinP1I	GCGC	1 cut(s)	367
HincII	GTYRAC	1 cut(s)	946
HindII	GTYRAC	1 cut(s)	946
HindIII	AAGCTT	2 cut(s)	440, 1375
HinfI	GANTC	6 cut(s)	68, 128, 333, 635, 739, 1020
HpaII	CCGG	1 cut(s)	803
HphI	GGTGA	2 cut(s)	269, 1096
Hpy166II	GTNNAC	3 cut(s)	819, 946, 1137
Hpy188I	TCNGA	4 cut(s)	262, 418, 559, 960
Hpy188III	TCNNGA	9 cut(s)	272, 409, 632, 785, 928, 1033, 1155, 1420, 1462
Hpy8I	GTNNAC	3 cut(s)	819, 946, 1137
HpyAV	CCTTC	4 cut(s)	973, 1333, 1393, 1463
HpyCH4III	ACNGT	4 cut(s)	187, 358, 1324, 1336
HpyCH4IV	ACGT	1 cut(s)	107
HpyCH4V	TGCA	8 cut(s)	40, 205, 765, 812, 1109, 1267, 1350, 1435
HpyF10VI	GCNNNNNNNGC	6 cut(s)	211, 1115, 1273, 1363, 1436, 1481
HpyF3I	CTNAG	6 cut(s)	259, 415, 450, 1153, 1316, 1418
HpySE526I	ACGT	1 cut(s)	107
Hsp92I	GRCGYC	1 cut(s)	422
HspAI	GCGC	1 cut(s)	367
Ksp22I	TGATCA	2 cut(s)	274, 874
Kzo9I	GATC	6 cut(s)	228, 274, 411, 781, 787, 874
LmnI	GCTCC	1 cut(s)	287
Lsp1109I	GCAGC	3 cut(s)	52, 189, 1414
LweI	GCATC	3 cut(s)	999, 1298, 1381
MaeI	CTAG	4 cut(s)	351, 371, 885, 1277
MaeII	ACGT	1 cut(s)	107
MaeIII	GTNAC	3 cut(s)	322, 1111, 1399
MalI	GATC	6 cut(s)	230, 276, 413, 783, 789, 876
MboI	GATC	6 cut(s)	228, 274, 411, 781, 787, 874
MboII	GAAGA	3 cut(s)	184, 782, 1477
MflI	RGATCY	2 cut(s)	228, 411
MhlI	GDGCHC	3 cut(s)	496, 1235, 1284
MlsI	TGGCCA	1 cut(s)	315
MluCI	AATT	5 cut(s)	79, 678, 700, 837, 1360
MluNI	TGGCCA	1 cut(s)	315
MlyI	GAGTC	3 cut(s)	77, 122, 733
MmeI	TCCRAC	4 cut(s)	70, 846, 1052, 1355
Mox20I	TGGCCA	1 cut(s)	315
MroXI	GAANNNNTTC	2 cut(s)	380, 1148
MscI	TGGCCA	1 cut(s)	315
MseI	TTAA	2 cut(s)	528, 1488
MslI	CAYNNNNRTG	1 cut(s)	282
Msp20I	TGGCCA	1 cut(s)	315
MspI	CCGG	1 cut(s)	803
MspR9I	CCNGG	4 cut(s)	216, 239, 282, 773
MvaI	CCWGG	4 cut(s)	216, 239, 282, 773
MwoI	GCNNNNNNNGC	6 cut(s)	211, 1115, 1273, 1363, 1436, 1481
NcoI	CCATGG	1 cut(s)	1495
NdeII	GATC	6 cut(s)	228, 274, 411, 781, 787, 874
NlaIV	GGNNCC	5 cut(s)	221, 602, 619, 674, 1216
NmuCI	GTSAC	2 cut(s)	1111, 1399
NspI	RCATGY	1 cut(s)	802
NspV	TTCGAA	1 cut(s)	474
OliI	CACNNNNGTG	1 cut(s)	282
PagI	TCATGA	3 cut(s)	271, 631, 927
PdmI	GAANNNNTTC	2 cut(s)	380, 1148
PfeI	GAWTC	3 cut(s)	333, 635, 1020
PflMI	CCANNNNNTGG	1 cut(s)	1181
PkrI	GCNGC	3 cut(s)	42, 204, 1429
PleI	GAGTC	3 cut(s)	76, 122, 733
PpsI	GAGTC	3 cut(s)	76, 122, 733
PpuMI	RGGWCCY	2 cut(s)	220, 601
PshBI	ATTAAT	1 cut(s)	1488
Psp5II	RGGWCCY	2 cut(s)	220, 601
Psp6I	CCWGG	4 cut(s)	214, 237, 280, 771
PspFI	CCCAGC	1 cut(s)	695
PspGI	CCWGG	4 cut(s)	214, 237, 280, 771
PspN4I	GGNNCC	5 cut(s)	221, 602, 619, 674, 1216
PspPI	GGNCC	4 cut(s)	220, 571, 601, 1172
PspPPI	RGGWCCY	2 cut(s)	220, 601
PstI	CTGCAG	2 cut(s)	1269, 1437
PstNI	CAGNNNCTG	1 cut(s)	691
PsuI	RGATCY	2 cut(s)	228, 411
RsaI	GTAC	2 cut(s)	380, 1241
RsaNI	GTAC	2 cut(s)	379, 1240
RseI	CAYNNNNRTG	1 cut(s)	282
SaqAI	TTAA	2 cut(s)	528, 1488
SatI	GCNGC	3 cut(s)	41, 203, 1428
Sau3AI	GATC	6 cut(s)	228, 274, 411, 781, 787, 874
Sau96I	GGNCC	4 cut(s)	220, 571, 601, 1172
SbfI	CCTGCAGG	1 cut(s)	1437
ScaI	AGTACT	1 cut(s)	380
SchI	GAGTC	3 cut(s)	77, 122, 733
ScrFI	CCNGG	4 cut(s)	216, 239, 282, 773
SdaI	CCTGCAGG	1 cut(s)	1437
SduI	GDGCHC	3 cut(s)	496, 1235, 1284
SfaNI	GCATC	3 cut(s)	999, 1298, 1381
SfcI	CTRYAG	3 cut(s)	433, 1265, 1433
SfuI	TTCGAA	1 cut(s)	474
SinI	GGWCC	4 cut(s)	220, 571, 601, 1172
SmiMI	CAYNNNNRTG	1 cut(s)	282
SmlI	CTYRAG	1 cut(s)	398
SmoI	CTYRAG	1 cut(s)	398
Sse8387I	CCTGCAGG	1 cut(s)	1437
Sse9I	AATT	5 cut(s)	79, 678, 700, 837, 1360
SspI	AATATT	1 cut(s)	505
SspMI	CTAG	4 cut(s)	351, 371, 885, 1277
StyD4I	CCNGG	4 cut(s)	214, 237, 280, 771
StyI	CCWWGG	3 cut(s)	370, 426, 1495
TaaI	ACNGT	4 cut(s)	187, 358, 1324, 1336
TaiI	ACGT	1 cut(s)	110
TaqI	TCGA	2 cut(s)	408, 474
TasI	AATT	5 cut(s)	79, 678, 700, 837, 1360
TatI	WGTACW	2 cut(s)	378, 1239
TfiI	GAWTC	3 cut(s)	333, 635, 1020
Tru1I	TTAA	2 cut(s)	528, 1488
Tru9I	TTAA	2 cut(s)	528, 1488
TscAI	CASTG	3 cut(s)	361, 819, 1116
TseFI	GTSAC	2 cut(s)	1111, 1399
TseI	GCWGC	3 cut(s)	40, 202, 1427
Tsp45I	GTSAC	2 cut(s)	1111, 1399
TspDTI	ATGAA	6 cut(s)	133, 260, 325, 434, 821, 1022
TspRI	CASTG	3 cut(s)	361, 819, 1116
Van91I	CCANNNNNTGG	1 cut(s)	1181
VpaK11BI	GGWCC	4 cut(s)	220, 571, 601, 1172
VspI	ATTAAT	1 cut(s)	1488
XapI	RAATTY	1 cut(s)	79
XceI	RCATGY	1 cut(s)	802
XmaJI	CCTAGG	1 cut(s)	370
XmnI	GAANNNNTTC	2 cut(s)	380, 1148
XspI	CTAG	4 cut(s)	351, 371, 885, 1277
ZrmI	AGTACT	1 cut(s)	380

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.