pycom07g12700

Delta(8)-fatty-acid desaturase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0003231
Physical Location & Seq
Forward (+)
14031058 .. 14032254
1197 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g12700.1

Sequence Viewer

Length: 1197 bp
ATGTCCGGCCAGGACGTCACTGATGGATTCATTGCCTACCATCCTCCATCCGCATGGAAGTACCTGGGAAAGTTCTTCACTGGGTACTATCTGAAAGATTTCGAGATCTCGGAGGTCTCCAAGGATTACAGGAAGCTTGCTTCCGAGTTTAACCAGCTCGGCTTGTTTGAAAACAAAGGGCATGGGGCTCTTTACTCGGTGATTAGTATTCTGGTTATGCTTTACCTTGTTGTGTATGGTGTGTTGAAGTCTGAGAGTGTGTTGGTTCATTTCGGTTGCGGTTGTTTGTTGGGGGTCCTCTGGGTTCAGGGTGCCTATATGGGTCATGACTCCGGGCATTACCAGGTCATGTCTAGCCGCAGGTACAACAAACTCGCTCAATTTCTGGCCGGGAATTGCCTTACTGGGATCAGCATTGCTTGGTGGAAATGGACTCACAATGCCCATCACATTGCCTGCAACAGCCTTGATTATGATCCTGATCTTCAACACATGCCAGTTTTTGCCGTGTCCTCGAAATTCTTTCAGTCAATAACATCAACCTTTTACGGAAGAGAGCTGACGTTTGATGCCGTGGCTAGATTTTTAGTCAGCCACCAGCATTTTACGTATTATCCAATCATGATTGTTGGAAGGGTAAACTTGTTTATACAGACGTTCTTGTTATTGTTCTCGAAGAGGAGCATCCCAGATAGAGCTTTGAACATAATGGGGATCCTTGTTTTCTGGACTTGGTTTCCTCTCCTTGTTTCGTGTCTGCCTAATTGGAGCGAGAGGTTTATGTTTGTGTTGGCGAGCTTTACAGTGACAGCACTTCAGCACATTCAGTTCACTTTGAACCATTTTACAGGAGATACTTATCTTGGACCACCAACTGGCAACGATTGGTTTGAGAAGCAGGCAGCTGGTACCGTGGATATTTTGTGCTCGAGTAAGATGGACTGGTTCTATGGTGGCCTGCAGTTTCAGCTTGAGCATCATTTGTTCCCACGACTGCCCCGTTCCCAGCTGAGGAAGATTTCTCCCACTGTGAAGGAACTATGCAAGAAGCACAATTTACCTTACAAGAGTTTGTCCTTCTTGGATGCCAATGTGACTACCATTAGGATGCTAAGGAATGCTGCCCAGCAGGCTTGGGACATGGCCCTTCCCGTCCCCAAGAACTTGGCCTGGGAAGCTGTCAATACCCATGGTTGA

Protein Analysis

399

Amino Acids

45.57

Weight (kDa)

8.81

Isoelectric Point (pI)

43.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase PF00487 88 - 357 1.7e-33 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 18
Acc36I ACCTGC 1 cut(s) 351
Acc65I GGTACC 1 cut(s) 908
AccB1I GGYRCC 2 cut(s) 311, 908
AccB7I CCANNNNNTGG 1 cut(s) 875
AciI CCGC 3 cut(s) 51, 279, 358
AclWI GGATC 4 cut(s) 416, 470, 709, 722
AcoI YGGCCR 2 cut(s) 7, 387
AcsI RAATTY 1 cut(s) 518
AcuI CTGAAG 1 cut(s) 800
AcyI GRCGYC 1 cut(s) 15
AfaI GTAC 4 cut(s) 62, 86, 365, 910
AfiI CCNNNNNNNGG 2 cut(s) 875, 1011
AgsI TTSAA 5 cut(s) 170, 247, 488, 703, 838
AjnI CCWGG 4 cut(s) 9, 63, 342, 1169
AluBI AGCT 9 cut(s) 136, 157, 559, 698, 798, 905, 970, 1009, 1178
AluI AGCT 9 cut(s) 136, 157, 559, 698, 798, 905, 970, 1009, 1178
Alw21I GWGCWC 1 cut(s) 929
Alw26I GTCTC 1 cut(s) 121
AlwI GGATC 4 cut(s) 416, 470, 709, 722
Ama87I CYCGRG 1 cut(s) 928
AoxI GGCC 5 cut(s) 7, 387, 955, 1143, 1167
ApeKI GCWGC 2 cut(s) 902, 1121
ApoI RAATTY 1 cut(s) 518
Asp700I GAANNNNTTC 1 cut(s) 98
Asp718I GGTACC 1 cut(s) 908
AspS9I GGNCC 3 cut(s) 295, 866, 1144
AsuC2I CCSGG 2 cut(s) 334, 391
AsuHPI GGTGA 1 cut(s) 211
AvaI CYCGRG 1 cut(s) 928
AvaII GGWCC 2 cut(s) 295, 866
BamHI GGATCC 1 cut(s) 714
BanI GGYRCC 2 cut(s) 311, 908
BanII GRGCYC 1 cut(s) 190
Bbv12I GWGCWC 1 cut(s) 929
BbvCI CCTCAGC 1 cut(s) 1010
BbvI GCAGC 2 cut(s) 914, 1108
BccI CCATC 5 cut(s) 17, 48, 55, 453, 931
BceAI ACGGC 2 cut(s) 491, 557
BciT130I CCWGG 4 cut(s) 11, 65, 344, 1171
BcnI CCSGG 2 cut(s) 334, 391
BcoDI GTCTC 1 cut(s) 121
BfaI CTAG 2 cut(s) 354, 579
BfmI CTRYAG 1 cut(s) 959
BfuAI ACCTGC 1 cut(s) 351
BglI GCCNNNNNGGC 1 cut(s) 1130
BglII AGATCT 1 cut(s) 105
BisI GCNGC 3 cut(s) 358, 903, 1122
BlsI GCNGC 3 cut(s) 359, 904, 1123
Bme1390I CCNGG 6 cut(s) 11, 65, 334, 344, 391, 1171
Bme18I GGWCC 2 cut(s) 295, 866
BmeT110I CYCGRG 1 cut(s) 928
BmgT120I GGNCC 3 cut(s) 295, 866, 1144
BmiI GGNNCC 4 cut(s) 296, 313, 716, 910
BmrFI CCNGG 6 cut(s) 11, 65, 334, 344, 391, 1171
BmrI ACTGGG 2 cut(s) 90, 414
BmsI GCATC 5 cut(s) 559, 693, 985, 1075, 1098
BmuI ACTGGG 2 cut(s) 90, 414
Bpu10I CCTNAGC 2 cut(s) 1010, 1112
BpuEI CTTGAG 1 cut(s) 992
BpuMI CCSGG 2 cut(s) 334, 391
BsaAI YACGTR 1 cut(s) 609
BsaBI GATNNNNATC 3 cut(s) 474, 480, 858
BsaHI GRCGYC 1 cut(s) 15
BsaI GGTCTC 1 cut(s) 121
BsaJI CCNNGG 6 cut(s) 64, 120, 573, 912, 1170, 1189
Bsc4I CCNNNNNNNGG 2 cut(s) 875, 1011
Bse1I ACTGG 5 cut(s) 85, 409, 497, 880, 947
Bse3DI GCAATG 3 cut(s) 30, 414, 450
Bse8I GATNNNNATC 3 cut(s) 474, 480, 858
BseBI CCWGG 4 cut(s) 11, 65, 344, 1171
BseDI CCNNGG 6 cut(s) 64, 120, 573, 912, 1170, 1189
BseGI GGATG 5 cut(s) 40, 47, 684, 1090, 1113
BseJI GATNNNNATC 3 cut(s) 474, 480, 858
BseLI CCNNNNNNNGG 2 cut(s) 875, 1011
BseMI GCAATG 3 cut(s) 30, 414, 450
BseMII CTCAG 2 cut(s) 243, 1001
BseNI ACTGG 5 cut(s) 85, 409, 497, 880, 947
BseRI GAGGAG 1 cut(s) 694
BseXI GCAGC 2 cut(s) 914, 1108
BseYI CCCAGC 2 cut(s) 1005, 1125
BshFI GGCC 5 cut(s) 9, 389, 957, 1145, 1169
BshNI GGYRCC 2 cut(s) 311, 908
BsiHKAI GWGCWC 1 cut(s) 929
BsiHKCI CYCGRG 1 cut(s) 928
BsiSI CCGG 3 cut(s) 6, 333, 390
BslFI GGGAC 2 cut(s) 1139, 1151
BslI CCNNNNNNNGG 2 cut(s) 875, 1011
BsmAI GTCTC 1 cut(s) 121
BsmFI GGGAC 2 cut(s) 1139, 1151
BsmI GAATGC 1 cut(s) 1123
BsnI GGCC 5 cut(s) 9, 389, 957, 1145, 1169
Bso31I GGTCTC 1 cut(s) 121
BsoBI CYCGRG 1 cut(s) 928
Bsp1286I GDGCHC 2 cut(s) 190, 929
Bsp143I GATC 5 cut(s) 105, 408, 475, 481, 714
Bsp19I CCATGG 1 cut(s) 1189
BspACI CCGC 3 cut(s) 51, 279, 358
BspANI GGCC 5 cut(s) 9, 389, 957, 1145, 1169
BspCNI CTCAG 2 cut(s) 244, 1002
BspHI TCATGA 2 cut(s) 325, 621
BspLI GGNNCC 4 cut(s) 296, 313, 716, 910
BspMAI CTGCAG 1 cut(s) 963
BspMI ACCTGC 1 cut(s) 351
BspPI GGATC 4 cut(s) 416, 470, 709, 722
BspT107I GGYRCC 2 cut(s) 311, 908
BspTNI GGTCTC 1 cut(s) 121
BsrDI GCAATG 3 cut(s) 30, 414, 450
BsrI ACTGG 5 cut(s) 85, 409, 497, 880, 947
BssECI CCNNGG 6 cut(s) 64, 120, 573, 912, 1170, 1189
BssMI GATC 5 cut(s) 105, 408, 475, 481, 714
BssNI GRCGYC 1 cut(s) 15
BssT1I CCWWGG 2 cut(s) 120, 1189
Bst2UI CCWGG 4 cut(s) 11, 65, 344, 1171
Bst4CI ACNGT 3 cut(s) 805, 913, 1030
Bst6I CTCTTC 2 cut(s) 547, 671
BstACI GRCGYC 1 cut(s) 15
BstBAI YACGTR 1 cut(s) 609
BstC8I GCNNGC 6 cut(s) 138, 457, 796, 900, 959, 1131
BstDEI CTNAG 3 cut(s) 252, 1010, 1112
BstDSI CCRYGG 3 cut(s) 573, 912, 1189
BstF5I GGATG 5 cut(s) 40, 47, 684, 1090, 1113
BstKTI GATC 5 cut(s) 108, 411, 478, 484, 717
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 5 cut(s) 105, 408, 475, 481, 714
BstMWI GCNNNNNNNGC 3 cut(s) 967, 1130, 1175
BstNI CCWGG 4 cut(s) 11, 65, 344, 1171
BstNSI RCATGY 1 cut(s) 496
BstSCI CCNGG 6 cut(s) 9, 63, 332, 342, 389, 1169
BstSFI CTRYAG 1 cut(s) 959
BstSNI TACGTA 1 cut(s) 609
BstV1I GCAGC 2 cut(s) 914, 1108
BstX2I RGATCY 2 cut(s) 105, 714
BstXI CCANNNNNNTGG 2 cut(s) 54, 1165
BstYI RGATCY 2 cut(s) 105, 714
BsuRI GGCC 5 cut(s) 9, 389, 957, 1145, 1169
BtgI CCRYGG 3 cut(s) 573, 912, 1189
BtsCI GGATG 5 cut(s) 40, 47, 684, 1090, 1113
BtsIMutI CAGTG 4 cut(s) 18, 78, 810, 1026
BveI ACCTGC 1 cut(s) 351
Cac8I GCNNGC 6 cut(s) 138, 457, 796, 900, 959, 1131
CciI TCATGA 2 cut(s) 325, 621
Cfr13I GGNCC 3 cut(s) 295, 866, 1144
CsiI ACCWGGT 1 cut(s) 342
Csp6I GTAC 4 cut(s) 61, 85, 364, 909
CviAII CATG 8 cut(s) 54, 182, 326, 349, 493, 622, 1141, 1190
CviQI GTAC 4 cut(s) 61, 85, 364, 909
DdeI CTNAG 3 cut(s) 252, 1010, 1112
DpnI GATC 5 cut(s) 107, 410, 477, 483, 716
DpnII GATC 5 cut(s) 105, 408, 475, 481, 714
EaeI YGGCCR 2 cut(s) 7, 387
Eam1104I CTCTTC 2 cut(s) 547, 671
EarI CTCTTC 2 cut(s) 547, 671
Eco105I TACGTA 1 cut(s) 609
Eco130I CCWWGG 2 cut(s) 120, 1189
Eco24I GRGCYC 1 cut(s) 190
Eco31I GGTCTC 1 cut(s) 121
Eco47I GGWCC 2 cut(s) 295, 866
Eco57I CTGAAG 1 cut(s) 800
Eco88I CYCGRG 1 cut(s) 928
EcoO109I RGGNCCY 1 cut(s) 295
EcoRII CCWGG 4 cut(s) 9, 63, 342, 1169
EcoT14I CCWWGG 2 cut(s) 120, 1189
EcoT38I GRGCYC 1 cut(s) 190
ErhI CCWWGG 2 cut(s) 120, 1189
FaeI CATG 8 cut(s) 57, 185, 329, 352, 496, 625, 1144, 1193
FaqI GGGAC 2 cut(s) 1139, 1151
FatI CATG 8 cut(s) 53, 181, 325, 348, 492, 621, 1140, 1189
Fnu4HI GCNGC 3 cut(s) 358, 903, 1122
FokI GGATG 5 cut(s) 27, 34, 671, 1097, 1120
FriOI GRGCYC 1 cut(s) 190
Fsp4HI GCNGC 3 cut(s) 358, 903, 1122
FspBI CTAG 2 cut(s) 354, 579
GluI GCNGC 3 cut(s) 358, 903, 1122
GsaI CCCAGC 2 cut(s) 1009, 1129
HaeIII GGCC 5 cut(s) 9, 389, 957, 1145, 1169
HapII CCGG 3 cut(s) 6, 333, 390
Hin1I GRCGYC 1 cut(s) 15
Hin1II CATG 8 cut(s) 57, 185, 329, 352, 496, 625, 1144, 1193
HindIII AAGCTT 1 cut(s) 134
HinfI GANTC 3 cut(s) 27, 329, 433
HpaII CCGG 3 cut(s) 6, 333, 390
HphI GGTGA 1 cut(s) 211
Hpy166II GTNNAC 2 cut(s) 640, 831
Hpy188I TCNGA 4 cut(s) 93, 112, 145, 253
Hpy188III TCNNGA 6 cut(s) 103, 326, 479, 622, 673, 727
Hpy8I GTNNAC 2 cut(s) 640, 831
HpyAV CCTTC 4 cut(s) 627, 1027, 1087, 1157
HpyCH4III ACNGT 3 cut(s) 805, 913, 1030
HpyCH4IV ACGT 4 cut(s) 15, 563, 608, 656
HpyCH4V TGCA 3 cut(s) 459, 961, 1044
HpyF10VI GCNNNNNNNGC 3 cut(s) 967, 1130, 1175
HpyF3I CTNAG 3 cut(s) 252, 1010, 1112
HpySE526I ACGT 4 cut(s) 15, 563, 608, 656
Hsp92I GRCGYC 1 cut(s) 15
Hsp92II CATG 8 cut(s) 57, 185, 329, 352, 496, 625, 1144, 1193
KpnI GGTACC 1 cut(s) 912
Kzo9I GATC 5 cut(s) 105, 408, 475, 481, 714
LmnI GCTCC 2 cut(s) 681, 768
Lsp1109I GCAGC 2 cut(s) 914, 1108
LweI GCATC 5 cut(s) 559, 693, 985, 1075, 1098
MabI ACCWGGT 1 cut(s) 342
MaeI CTAG 2 cut(s) 354, 579
MaeII ACGT 4 cut(s) 15, 563, 608, 656
MaeIII GTNAC 3 cut(s) 16, 805, 1093
MalI GATC 5 cut(s) 107, 410, 477, 483, 716
MboI GATC 5 cut(s) 105, 408, 475, 481, 714
MboII GAAGA 5 cut(s) 67, 476, 564, 688, 1027
MflI RGATCY 2 cut(s) 105, 714
MhlI GDGCHC 2 cut(s) 190, 929
MluCI AATT 5 cut(s) 380, 394, 518, 763, 1054
MlyI GAGTC 2 cut(s) 323, 427
MmeI TCCRAC 1 cut(s) 610
MnlI CCTC 8 cut(s) 54, 106, 308, 523, 672, 750, 768, 1005
MroXI GAANNNNTTC 1 cut(s) 98
MseI TTAA 1 cut(s) 150
MslI CAYNNNNRTG 2 cut(s) 52, 1106
MspA1I CMGCKG 2 cut(s) 905, 1009
MspI CCGG 3 cut(s) 6, 333, 390
MspR9I CCNGG 6 cut(s) 11, 65, 334, 344, 391, 1171
Mva1269I GAATGC 1 cut(s) 1123
MvaI CCWGG 4 cut(s) 11, 65, 344, 1171
MwoI GCNNNNNNNGC 3 cut(s) 967, 1130, 1175
NciI CCSGG 2 cut(s) 334, 391
NcoI CCATGG 1 cut(s) 1189
NdeII GATC 5 cut(s) 105, 408, 475, 481, 714
NlaIII CATG 8 cut(s) 57, 185, 329, 352, 496, 625, 1144, 1193
NlaIV GGNNCC 4 cut(s) 296, 313, 716, 910
NmeAIII GCCGAG 1 cut(s) 138
NmuCI GTSAC 3 cut(s) 16, 805, 1093
NspI RCATGY 1 cut(s) 496
PaeR7I CTCGAG 1 cut(s) 928
PagI TCATGA 2 cut(s) 325, 621
PcsI WCGNNNNNNNCGW 1 cut(s) 997
PctI GAATGC 1 cut(s) 1123
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 1 cut(s) 27
PflMI CCANNNNNTGG 1 cut(s) 875
PkrI GCNGC 3 cut(s) 359, 904, 1123
PleI GAGTC 2 cut(s) 323, 427
PpsI GAGTC 2 cut(s) 323, 427
Ppu21I YACGTR 1 cut(s) 609
PpuMI RGGWCCY 1 cut(s) 295
Psp5II RGGWCCY 1 cut(s) 295
Psp6I CCWGG 4 cut(s) 9, 63, 342, 1169
PspFI CCCAGC 2 cut(s) 1005, 1125
PspGI CCWGG 4 cut(s) 9, 63, 342, 1169
PspN4I GGNNCC 4 cut(s) 296, 313, 716, 910
PspPI GGNCC 3 cut(s) 295, 866, 1144
PspPPI RGGWCCY 1 cut(s) 295
PspXI VCTCGAGB 1 cut(s) 928
PstI CTGCAG 1 cut(s) 963
PsuI RGATCY 2 cut(s) 105, 714
PvuII CAGCTG 2 cut(s) 905, 1009
RsaI GTAC 4 cut(s) 62, 86, 365, 910
RsaNI GTAC 4 cut(s) 61, 85, 364, 909
RseI CAYNNNNRTG 2 cut(s) 52, 1106
SaqAI TTAA 1 cut(s) 150
SatI GCNGC 3 cut(s) 358, 903, 1122
Sau3AI GATC 5 cut(s) 105, 408, 475, 481, 714
Sau96I GGNCC 3 cut(s) 295, 866, 1144
SchI GAGTC 2 cut(s) 323, 427
ScrFI CCNGG 6 cut(s) 11, 65, 334, 344, 391, 1171
SduI GDGCHC 2 cut(s) 190, 929
SexAI ACCWGGT 1 cut(s) 342
SfaNI GCATC 5 cut(s) 559, 693, 985, 1075, 1098
SfcI CTRYAG 1 cut(s) 959
Sfr274I CTCGAG 1 cut(s) 928
SinI GGWCC 2 cut(s) 295, 866
SlaI CTCGAG 1 cut(s) 928
SmiMI CAYNNNNRTG 2 cut(s) 52, 1106
SmlI CTYRAG 2 cut(s) 928, 971
SmoI CTYRAG 2 cut(s) 928, 971
SnaBI TACGTA 1 cut(s) 609
Sse9I AATT 5 cut(s) 380, 394, 518, 763, 1054
SsiI CCGC 3 cut(s) 51, 279, 358
SspMI CTAG 2 cut(s) 354, 579
StyD4I CCNGG 6 cut(s) 9, 63, 332, 342, 389, 1169
StyI CCWWGG 2 cut(s) 120, 1189
TaaI ACNGT 3 cut(s) 805, 913, 1030
TaiI ACGT 4 cut(s) 18, 566, 611, 659
TaqI TCGA 4 cut(s) 102, 515, 674, 929
TasI AATT 5 cut(s) 380, 394, 518, 763, 1054
TauI GCSGC 1 cut(s) 360
TfiI GAWTC 1 cut(s) 27
Tru1I TTAA 1 cut(s) 150
Tru9I TTAA 1 cut(s) 150
TscAI CASTG 4 cut(s) 25, 85, 810, 1033
TseFI GTSAC 3 cut(s) 16, 805, 1093
TseI GCWGC 2 cut(s) 902, 1121
Tsp45I GTSAC 3 cut(s) 16, 805, 1093
TspDTI ATGAA 2 cut(s) 19, 257
TspGWI ACGGA 1 cut(s) 564
TspRI CASTG 4 cut(s) 25, 85, 810, 1033
Van91I CCANNNNNTGG 1 cut(s) 875
VpaK11BI GGWCC 2 cut(s) 295, 866
XapI RAATTY 1 cut(s) 518
XceI RCATGY 1 cut(s) 496
XhoI CTCGAG 1 cut(s) 928
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 2 cut(s) 354, 579
ZraI GACGTC 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.