Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1A

Orthogroup ID

OG0003231

Physical Location & Seq

Genomic Coordinates Reverse (-)

50139903 .. 50143571

Gene Structure 3669 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1AG273500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1356 bp

ATGGAGGAGAAAATGGAGGTTGAGAACAAGTATATTACTGTTGAGGAGCTGAAGCTGCACAACAAGCCTGGGGACCTATGGATCTCTATCCAGGGCAAGATTTACAATGTCTCAGATTGGGTTCATGATCACCCAGGTGGAGCTACCATTTTGTATGATTTGGGTGGCCAAGATGTTACTGATGGATTCATGGCATACCATCCTAGCACTGTGTGGAAGCGCCTAGGGAAGTACTTCAATGGGTATCATCTCAAGGATTTCGAGATCTCAGAGGCGTCCAAGGACTATAGGAAGCTTCATGCTGAGATTACGCAGATGGGTTTGTTCGAAAACAAAGGGCATGGGGCTCTTTACAATATTACAACTATTGTTGTTTTGCTTAACCTTGTTGTTTATGGTGTATTGAAGTCTGATAGTGTGTTGGTCCATTTTGTTTGTGCTGTGATGTTGGGGATCTTGTGGATTATGGGTGCTTATATGGGTCATGATGCGGGTCATTACCAAGTCATGTCAAGCCGTAAGTGGAACCAAATTATTCAGGTTCTGGCTGGGAATTGCCTTACTGGGATTAGCATTGCTTGGTGGAAATGGACTCACAACGCACATCATATTGCCTGCAACAGCCTGGATTATGATCCTGATCTTCAACACATGCCGGTCTTTGCAGTGTCGACAAAGTTCTTTCATTCGATTACTTCAGTCTTTTATGGGAGGAAGTTGGATTTTGATCATGTGGCTAGGTTCTTAGTCAGCCATCAGCATTTTACTTATTATCCTATCATGATTGTTGGAAGGGTCAACTTGTTTATTCAGACCTTCTTGTTGTTGTTGTCCAAGAGAAGCATCCCCAACAGGACTATGAACATAATGGGAATCCTTGTTTTCTGGACTTGGTTTCCTCTGCTTGTCATGTGTCTGCCCAGTTGGAGTGAGAGGGTGATGTTCGTGCTTGTGAGCTTTGCAGTGACAGCACTTCAGCACATTCAGTTCACTTTGAACCATTTCTCAGGAGATACTTATCTTGGACCACCAACTGGGAATGATTGGTTCGAGAAGCAGACTGCTGGAACCATTGATATTTCGTGCTCAAAGTACATGGATTGGTTCTTTGGTGGTCTGCAGTTTCAGCTAGAGCACCATTTGTTCCCCCGATTACCCCGATGCCAACTGAGGACAGTTTCTCCAACCGTGAAGGAACTATGCAAGAAGCACAAGTTGCCTCACAGAAGCTTGTCCTTCTGGGATGCCAATGTGACCACCATTAGGGTTCTCAGGAAAGCTGCCCTGCAGGCTTGGGACATGACCCTTCCTGTCCCGAAGAACTTGGCTTGGGAAGCTATTAATACCCATGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006629 GO:0006643 GO:0006665 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009266 GO:0009409 GO:0009628 GO:0009987 GO:0012505 GO:0016491 GO:0016705 GO:0030148 GO:0033554 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046467 GO:0050896 GO:0051716 GO:0052631 GO:0055114 GO:0070417 GO:0071704 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cyt-b5 FA_desaturase

Protein Analysis

451

Amino Acids

51.97

Weight (kDa)

8.52

Isoelectric Point (pI)

38.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cyt-b5	PF00173	13 - 82	6.3e-21	Cytochrome b5-like Heme/Steroid binding domain
FA_desaturase	PF00487	141 - 410	5.2e-33	Fatty acid desaturase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003231)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46210 AT3G61580
fragaria_vesca	FvH4_7g15550 FvH4_7g15550 FvH4_7g15570
malus_domestica	MD01G1072900.v1.1 MD01G1073000.v1.1 MD07G1129700.v1.1 MD07G1130000.v1.1
prunus_persica	Prupe.2G179200_v2.0.a1 Prupe.2G179200_v2.0.a1 Prupe.2G179400_v2.0.a1
pyrus_communis	pycom01g10190 pycom01g10200 pycom07g12680 pycom07g12700
rosa_chinensis	RchiOBHm_Chr1g0358201 RchiOBHm_Chr1g0358241
rosa_laevigata	RLG00000027980 RLG00000027983
rosa_multiflora	Rmu_sc0002071.1_g000009 Rmu_sc0007072.1_g000001 Rmu_sc0023145.1_g000004
rosa_roxburghii	Rroxscaffold_4G00298340 Rroxscaffold_4G00298370
rosa_rugosa	Rorug01G0259800 Rorug01G0259900
rosa_samantha	Rh1AG273500 Rh1AG273900 Rh1BG240700 Rh1BG241000 Rh1CG257100 Rh1CG257500 Rh1DG269600 Rh1DG269700
rosa_wichuraiana	Rw1G024450 Rw1G024480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1034
AccI	GTMKAC	1 cut(s)	671
AciI	CCGC	1 cut(s)	491
AclWI	GGATC	3 cut(s)	89, 461, 629
AcoI	YGGCCR	1 cut(s)	166
AcuI	CTGAAG	3 cut(s)	71, 681, 959
AcyI	GRCGYC	1 cut(s)	275
AdeI	CACNNNGTG	1 cut(s)	213
AfaI	GTAC	2 cut(s)	233, 1094
AfiI	CCNNNNNNNGG	2 cut(s)	1034, 1263
AgsI	TTSAA	4 cut(s)	238, 406, 647, 997
AjnI	CCWGG	4 cut(s)	67, 90, 133, 624
AleI	CACNNNNGTG	1 cut(s)	135
AluBI	AGCT	9 cut(s)	49, 55, 143, 295, 957, 1129, 1230, 1280, 1337
AluI	AGCT	9 cut(s)	49, 55, 143, 295, 957, 1129, 1230, 1280, 1337
Alw21I	GWGCWC	2 cut(s)	1088, 1137
Alw26I	GTCTC	1 cut(s)	115
AlwI	GGATC	3 cut(s)	89, 461, 629
AlwNI	CAGNNNCTG	1 cut(s)	544
AoxI	GGCC	1 cut(s)	166
ApeKI	GCWGC	2 cut(s)	55, 1280
AseI	ATTAAT	1 cut(s)	1341
Asp700I	GAANNNNTTC	2 cut(s)	233, 1001
AspA2I	CCTAGG	1 cut(s)	223
AspLEI	GCGC	1 cut(s)	222
AspS9I	GGNCC	3 cut(s)	73, 424, 1025
AsuHPI	GGTGA	2 cut(s)	122, 949
AsuII	TTCGAA	1 cut(s)	327
AvaII	GGWCC	3 cut(s)	73, 424, 1025
AvrII	CCTAGG	1 cut(s)	223
BalI	TGGCCA	1 cut(s)	168
BanII	GRGCYC	1 cut(s)	349
Bbv12I	GWGCWC	2 cut(s)	1088, 1137
BbvI	GCAGC	2 cut(s)	42, 1267
BccI	CCATC	4 cut(s)	176, 207, 310, 762
BceAI	ACGGC	1 cut(s)	501
BciT130I	CCWGG	4 cut(s)	69, 92, 135, 626
BclI	TGATCA	2 cut(s)	127, 727
BcoDI	GTCTC	1 cut(s)	115
BfaI	CTAG	4 cut(s)	204, 224, 738, 1130
BfmI	CTRYAG	3 cut(s)	286, 1118, 1286
BfoI	RGCGCY	1 cut(s)	223
BglI	GCCNNNNNGGC	1 cut(s)	1289
BglII	AGATCT	1 cut(s)	264
BisI	GCNGC	2 cut(s)	56, 1281
BlnI	CCTAGG	1 cut(s)	223
BlsI	GCNGC	2 cut(s)	57, 1282
BmcAI	AGTACT	1 cut(s)	233
Bme1390I	CCNGG	4 cut(s)	69, 92, 135, 626
Bme18I	GGWCC	3 cut(s)	73, 424, 1025
BmgT120I	GGNCC	3 cut(s)	73, 424, 1025
BmiI	GGNNCC	3 cut(s)	74, 527, 1069
BmrFI	CCNGG	4 cut(s)	69, 92, 135, 626
BmrI	ACTGGG	3 cut(s)	573, 915, 1044
BmsI	GCATC	4 cut(s)	478, 852, 1151, 1234
BmuI	ACTGGG	3 cut(s)	573, 915, 1044
Bpu14I	TTCGAA	1 cut(s)	327
BpuEI	CTTGAG	1 cut(s)	236
BsaBI	GATNNNNATC	5 cut(s)	86, 633, 639, 726, 1017
BsaHI	GRCGYC	1 cut(s)	275
BsaJI	CCNNGG	6 cut(s)	68, 91, 133, 223, 279, 1348
BsaXI	ACNNNNNCTCC	2 cut(s)	1165, 1195
Bsc4I	CCNNNNNNNGG	2 cut(s)	1034, 1263
Bse118I	RCCGGY	1 cut(s)	655
Bse1I	ACTGG	3 cut(s)	568, 921, 1039
Bse3DI	GCAATG	1 cut(s)	573
Bse8I	GATNNNNATC	5 cut(s)	86, 633, 639, 726, 1017
BseBI	CCWGG	4 cut(s)	69, 92, 135, 626
BseDI	CCNNGG	6 cut(s)	68, 91, 133, 223, 279, 1348
BseGI	GGATG	3 cut(s)	199, 843, 1249
BseJI	GATNNNNATC	5 cut(s)	86, 633, 639, 726, 1017
BseLI	CCNNNNNNNGG	2 cut(s)	1034, 1263
BseMI	GCAATG	1 cut(s)	573
BseMII	CTCAG	6 cut(s)	126, 282, 294, 1020, 1160, 1285
BseNI	ACTGG	3 cut(s)	568, 921, 1039
BseRI	GAGGAG	2 cut(s)	20, 59
BseXI	GCAGC	2 cut(s)	42, 1267
BseYI	CCCAGC	1 cut(s)	548
BsgI	GTGCAG	1 cut(s)	41
BshFI	GGCC	1 cut(s)	168
BsiHKAI	GWGCWC	2 cut(s)	1088, 1137
BsiSI	CCGG	1 cut(s)	656
BslFI	GGGAC	3 cut(s)	86, 1298, 1310
BslI	CCNNNNNNNGG	2 cut(s)	1034, 1263
BsmAI	GTCTC	1 cut(s)	115
BsmFI	GGGAC	3 cut(s)	86, 1298, 1310
BsnI	GGCC	1 cut(s)	168
Bsp119I	TTCGAA	1 cut(s)	327
Bsp1286I	GDGCHC	3 cut(s)	349, 1088, 1137
Bsp143I	GATC	7 cut(s)	81, 127, 264, 453, 634, 640, 727
Bsp19I	CCATGG	1 cut(s)	1348
BspACI	CCGC	1 cut(s)	491
BspANI	GGCC	1 cut(s)	168
BspCNI	CTCAG	6 cut(s)	125, 281, 295, 1019, 1161, 1284
BspHI	TCATGA	3 cut(s)	124, 484, 780
BspLI	GGNNCC	3 cut(s)	74, 527, 1069
BspMAI	CTGCAG	2 cut(s)	1122, 1290
BspPI	GGATC	3 cut(s)	89, 461, 629
BspT104I	TTCGAA	1 cut(s)	327
BsrDI	GCAATG	1 cut(s)	573
BsrFI	RCCGGY	1 cut(s)	655
BsrI	ACTGG	3 cut(s)	568, 921, 1039
BssAI	RCCGGY	1 cut(s)	655
BssECI	CCNNGG	6 cut(s)	68, 91, 133, 223, 279, 1348
BssMI	GATC	7 cut(s)	81, 127, 264, 453, 634, 640, 727
BssNI	GRCGYC	1 cut(s)	275
BssT1I	CCWWGG	3 cut(s)	223, 279, 1348
Bst2UI	CCWGG	4 cut(s)	69, 92, 135, 626
Bst4CI	ACNGT	4 cut(s)	40, 211, 1177, 1189
BstACI	GRCGYC	1 cut(s)	275
BstAPI	GCANNNNNTGC	1 cut(s)	1216
BstBI	TTCGAA	1 cut(s)	327
BstC8I	GCNNGC	2 cut(s)	616, 1290
BstDEI	CTNAG	7 cut(s)	112, 268, 303, 745, 1006, 1169, 1271
BstDSI	CCRYGG	1 cut(s)	1348
BstF5I	GGATG	3 cut(s)	199, 843, 1249
BstH2I	RGCGCY	1 cut(s)	223
BstHHI	GCGC	1 cut(s)	222
BstKTI	GATC	7 cut(s)	84, 130, 267, 456, 637, 643, 730
BstMAI	GTCTC	1 cut(s)	115
BstMBI	GATC	7 cut(s)	81, 127, 264, 453, 634, 640, 727
BstMWI	GCNNNNNNNGC	7 cut(s)	55, 64, 968, 1126, 1216, 1289, 1334
BstNI	CCWGG	4 cut(s)	69, 92, 135, 626
BstNSI	RCATGY	1 cut(s)	655
BstSCI	CCNGG	4 cut(s)	67, 90, 133, 624
BstSFI	CTRYAG	3 cut(s)	286, 1118, 1286
BstV1I	GCAGC	2 cut(s)	42, 1267
BstX2I	RGATCY	3 cut(s)	81, 264, 453
BstYI	RGATCY	3 cut(s)	81, 264, 453
BsuRI	GGCC	1 cut(s)	168
BtgI	CCRYGG	1 cut(s)	1348
BtsCI	GGATG	3 cut(s)	199, 843, 1249
BtsI	GCAGTG	2 cut(s)	672, 969
BtsIMutI	CAGTG	3 cut(s)	207, 672, 969
Cac8I	GCNNGC	2 cut(s)	616, 1290
CaiI	CAGNNNCTG	1 cut(s)	544
CciI	TCATGA	3 cut(s)	124, 484, 780
CfoI	GCGC	1 cut(s)	222
Cfr10I	RCCGGY	1 cut(s)	655
Cfr13I	GGNCC	3 cut(s)	73, 424, 1025
CseI	GACGC	1 cut(s)	264
Csp6I	GTAC	2 cut(s)	232, 1093
CviQI	GTAC	2 cut(s)	232, 1093
DdeI	CTNAG	7 cut(s)	112, 268, 303, 745, 1006, 1169, 1271
DpnI	GATC	7 cut(s)	83, 129, 266, 455, 636, 642, 729
DpnII	GATC	7 cut(s)	81, 127, 264, 453, 634, 640, 727
DraIII	CACNNNGTG	1 cut(s)	213
EaeI	YGGCCR	1 cut(s)	166
Eco130I	CCWWGG	3 cut(s)	223, 279, 1348
Eco24I	GRGCYC	1 cut(s)	349
Eco47I	GGWCC	3 cut(s)	73, 424, 1025
Eco57I	CTGAAG	3 cut(s)	71, 681, 959
EcoO109I	RGGNCCY	1 cut(s)	73
EcoRII	CCWGG	4 cut(s)	67, 90, 133, 624
EcoT14I	CCWWGG	3 cut(s)	223, 279, 1348
EcoT38I	GRGCYC	1 cut(s)	349
ErhI	CCWWGG	3 cut(s)	223, 279, 1348
FaqI	GGGAC	3 cut(s)	86, 1298, 1310
FauI	CCCGC	1 cut(s)	484
FbaI	TGATCA	2 cut(s)	127, 727
FblI	GTMKAC	1 cut(s)	671
Fnu4HI	GCNGC	2 cut(s)	56, 1281
FokI	GGATG	3 cut(s)	186, 830, 1256
FriOI	GRGCYC	1 cut(s)	349
Fsp4HI	GCNGC	2 cut(s)	56, 1281
FspBI	CTAG	4 cut(s)	204, 224, 738, 1130
GlaI	GCGC	1 cut(s)	221
GluI	GCNGC	2 cut(s)	56, 1281
GsaI	CCCAGC	1 cut(s)	552
HaeII	RGCGCY	1 cut(s)	223
HaeIII	GGCC	1 cut(s)	168
HapII	CCGG	1 cut(s)	656
HgaI	GACGC	1 cut(s)	264
HhaI	GCGC	1 cut(s)	222
Hin1I	GRCGYC	1 cut(s)	275
Hin6I	GCGC	1 cut(s)	220
HinP1I	GCGC	1 cut(s)	220
HincII	GTYRAC	2 cut(s)	672, 799
HindII	GTYRAC	2 cut(s)	672, 799
HindIII	AAGCTT	2 cut(s)	293, 1228
HinfI	GANTC	3 cut(s)	186, 592, 873
HpaII	CCGG	1 cut(s)	656
HphI	GGTGA	2 cut(s)	122, 949
Hpy166II	GTNNAC	3 cut(s)	672, 799, 990
Hpy188I	TCNGA	4 cut(s)	115, 271, 412, 813
Hpy8I	GTNNAC	3 cut(s)	672, 799, 990
HpyAV	CCTTC	5 cut(s)	786, 826, 1186, 1246, 1316
HpyCH4III	ACNGT	4 cut(s)	40, 211, 1177, 1189
HpyCH4V	TGCA	7 cut(s)	58, 618, 665, 962, 1120, 1203, 1288
HpyF10VI	GCNNNNNNNGC	7 cut(s)	55, 64, 968, 1126, 1216, 1289, 1334
HpyF3I	CTNAG	7 cut(s)	112, 268, 303, 745, 1006, 1169, 1271
Hsp92I	GRCGYC	1 cut(s)	275
HspAI	GCGC	1 cut(s)	220
Ksp22I	TGATCA	2 cut(s)	127, 727
Kzo9I	GATC	7 cut(s)	81, 127, 264, 453, 634, 640, 727
LmnI	GCTCC	2 cut(s)	46, 140
Lsp1109I	GCAGC	2 cut(s)	42, 1267
LweI	GCATC	4 cut(s)	478, 852, 1151, 1234
MaeI	CTAG	4 cut(s)	204, 224, 738, 1130
MaeIII	GTNAC	3 cut(s)	175, 964, 1252
MalI	GATC	7 cut(s)	83, 129, 266, 455, 636, 642, 729
MboI	GATC	7 cut(s)	81, 127, 264, 453, 634, 640, 727
MboII	GAAGA	2 cut(s)	635, 1330
MflI	RGATCY	3 cut(s)	81, 264, 453
MhlI	GDGCHC	3 cut(s)	349, 1088, 1137
MlsI	TGGCCA	1 cut(s)	168
MluCI	AATT	2 cut(s)	531, 553
MluNI	TGGCCA	1 cut(s)	168
MlyI	GAGTC	1 cut(s)	586
MmeI	TCCRAC	4 cut(s)	699, 769, 905, 1208
MnlI	CCTC	8 cut(s)	10, 37, 265, 705, 909, 927, 1164, 1230
Mox20I	TGGCCA	1 cut(s)	168
MroXI	GAANNNNTTC	2 cut(s)	233, 1001
MscI	TGGCCA	1 cut(s)	168
MseI	TTAA	2 cut(s)	381, 1341
MslI	CAYNNNNRTG	1 cut(s)	135
Msp20I	TGGCCA	1 cut(s)	168
MspI	CCGG	1 cut(s)	656
MspR9I	CCNGG	4 cut(s)	69, 92, 135, 626
MvaI	CCWGG	4 cut(s)	69, 92, 135, 626
MwoI	GCNNNNNNNGC	7 cut(s)	55, 64, 968, 1126, 1216, 1289, 1334
NcoI	CCATGG	1 cut(s)	1348
NdeII	GATC	7 cut(s)	81, 127, 264, 453, 634, 640, 727
NlaIV	GGNNCC	3 cut(s)	74, 527, 1069
NmuCI	GTSAC	2 cut(s)	964, 1252
NspI	RCATGY	1 cut(s)	655
NspV	TTCGAA	1 cut(s)	327
OliI	CACNNNNGTG	1 cut(s)	135
PagI	TCATGA	3 cut(s)	124, 484, 780
PdmI	GAANNNNTTC	2 cut(s)	233, 1001
PfeI	GAWTC	2 cut(s)	186, 873
PflMI	CCANNNNNTGG	1 cut(s)	1034
PkrI	GCNGC	2 cut(s)	57, 1282
PleI	GAGTC	1 cut(s)	586
PpsI	GAGTC	1 cut(s)	586
PpuMI	RGGWCCY	1 cut(s)	73
PshBI	ATTAAT	1 cut(s)	1341
Psp5II	RGGWCCY	1 cut(s)	73
Psp6I	CCWGG	4 cut(s)	67, 90, 133, 624
PspFI	CCCAGC	1 cut(s)	548
PspGI	CCWGG	4 cut(s)	67, 90, 133, 624
PspN4I	GGNNCC	3 cut(s)	74, 527, 1069
PspPI	GGNCC	3 cut(s)	73, 424, 1025
PspPPI	RGGWCCY	1 cut(s)	73
PstI	CTGCAG	2 cut(s)	1122, 1290
PstNI	CAGNNNCTG	1 cut(s)	544
PsuI	RGATCY	3 cut(s)	81, 264, 453
RsaI	GTAC	2 cut(s)	233, 1094
RsaNI	GTAC	2 cut(s)	232, 1093
RseI	CAYNNNNRTG	1 cut(s)	135
SalI	GTCGAC	1 cut(s)	670
SaqAI	TTAA	2 cut(s)	381, 1341
SatI	GCNGC	2 cut(s)	56, 1281
Sau3AI	GATC	7 cut(s)	81, 127, 264, 453, 634, 640, 727
Sau96I	GGNCC	3 cut(s)	73, 424, 1025
SbfI	CCTGCAGG	1 cut(s)	1290
ScaI	AGTACT	1 cut(s)	233
SchI	GAGTC	1 cut(s)	586
ScrFI	CCNGG	4 cut(s)	69, 92, 135, 626
SdaI	CCTGCAGG	1 cut(s)	1290
SduI	GDGCHC	3 cut(s)	349, 1088, 1137
SfaNI	GCATC	4 cut(s)	478, 852, 1151, 1234
SfcI	CTRYAG	3 cut(s)	286, 1118, 1286
SfuI	TTCGAA	1 cut(s)	327
SinI	GGWCC	3 cut(s)	73, 424, 1025
SmiMI	CAYNNNNRTG	1 cut(s)	135
SmlI	CTYRAG	1 cut(s)	251
SmoI	CTYRAG	1 cut(s)	251
Sse8387I	CCTGCAGG	1 cut(s)	1290
Sse9I	AATT	2 cut(s)	531, 553
SsiI	CCGC	1 cut(s)	491
SspI	AATATT	1 cut(s)	358
SspMI	CTAG	4 cut(s)	204, 224, 738, 1130
StyD4I	CCNGG	4 cut(s)	67, 90, 133, 624
StyI	CCWWGG	3 cut(s)	223, 279, 1348
TaaI	ACNGT	4 cut(s)	40, 211, 1177, 1189
TaqI	TCGA	5 cut(s)	261, 327, 671, 689, 1050
TasI	AATT	2 cut(s)	531, 553
TatI	WGTACW	2 cut(s)	231, 1092
TfiI	GAWTC	2 cut(s)	186, 873
Tru1I	TTAA	2 cut(s)	381, 1341
Tru9I	TTAA	2 cut(s)	381, 1341
TscAI	CASTG	3 cut(s)	214, 672, 969
TseFI	GTSAC	2 cut(s)	964, 1252
TseI	GCWGC	2 cut(s)	55, 1280
Tsp45I	GTSAC	2 cut(s)	964, 1252
TspDTI	ATGAA	5 cut(s)	113, 178, 287, 674, 875
TspRI	CASTG	3 cut(s)	214, 672, 969
Van91I	CCANNNNNTGG	1 cut(s)	1034
VpaK11BI	GGWCC	3 cut(s)	73, 424, 1025
VspI	ATTAAT	1 cut(s)	1341
XceI	RCATGY	1 cut(s)	655
XmaJI	CCTAGG	1 cut(s)	223
XmiI	GTMKAC	1 cut(s)	671
XmnI	GAANNNNTTC	2 cut(s)	233, 1001
XspI	CTAG	4 cut(s)	204, 224, 738, 1130
ZrmI	AGTACT	1 cut(s)	233

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.