MD01G1098500.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007094
Physical Location & Seq
Forward (+)
21220620 .. 21224310
3691 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1098500.v1.1.491

Sequence Viewer

Length: 1878 bp
ATGTCGGGTCGAATGCTGAAGAAAGTTTTGAAGGAACAAGAAGAAGAAGACCGACACCTAATAGAATCCGAATCAGAAGGTGAAGAAGAAATCCAGCAGCTCAATGGAAAAGCTAAAATCAATCCCTTCGACCTCCTCACCGGCGGCGGCGGCGACGGCGACGACGACGATCAGGAGGATGAATCAGAAACTGTTGATGAACCCGTGGTTGTTTTGCCTCAGCTGAAAACGAAGGCTGGTGTAGCTTCAACCAACGATCAAAAATCAAAGAAGAAGAAAAAGAAGAAAAGCAAAGAGGCTTCTTCCTCTTCTTCGGCTGCTTCCAATGTCGAAAAGCCCTTGGATGGGATTCTTGAATCAATTTCTTTGGATGCCCCGGAAAAAGCAGCCAGCGGAAAAGGTTGTGAGAATTTGGCTAAGCCGTGTGCACCCTCTGTGCTGCAAGTGGATCCCAAGTATTTAAATGCTGACAATGAGCTGCGGAGAATCTTTGGCTCTAAGGTGGTAAAGTCCTTTGAGAAACATGAGCGGAGTGGTGGCAGTTCTAGACTGGTGCGTGGTGGAAGACGTGCTGCTCATATTCCTAGAAAAACTATACTTGTCACTGCATCAGAGCATTGGCATCGCTGGGATGGTTCTTTGTCCATGGAATTTCTTGAAGTGATTGATGGATATCACCACTTTCGATACGTACATTCCTCATCCTACCTTCAAGCTCAGAGGGCATTTGAAGCTGCCAAAGCAATACATGATATCAATGGAATTGCAAGTATTTTGTTATACAATCCTTATCATTTGGATTCGCTTATAACCATGGCAGAGTACTATAAGTTTTCGGGTGAACATCAAATATCAGCAGAACTTATCTCGAAGTGCTTATATGCTTTGGAATGTGCATGGAATCCAATGTTCACACCCTTGCAGGGCAATTGCCAACTAAAATACAGCCATGAAACAAACAAGCCTCTGTTCACGGTACTTTTTACTCACATGAGAAATATGGATAGGCGTGGTTGCCATCGATCTGCTTTAGAAGTCTGCAAGTTGTTACTTTCACTAGATTTTGATGATCCAATGGGGGCCATGTTCTGCATTGACTATTTTTCGTTGAGGGCAGAGGAGTATGCATGGCTAGAACGGTTCTCTGAAGAATATAAAAGTGATAACTCCTTATGGTTGTTTCCAAATTTTTCATATTCCCTTGCCATTTGCCATTTCTATCTTGAGAAAGAAGAATCTTCGAAAGGTGCACTGGAAAATGCCAAGTCTACTTCAGCTGATCTTATGAAACAGGCATTGATACTTCACCCGTCAGTCTTGAAAAAATTAGTGGAAAAGGTACCTTTGAAGGACAAGGTGTGGGCGCAGATACTAAAAAATCCATATTTTCAAGCAGATCAAGTAGGAATCCCATCCTTGGATCACCTAATAAACATATATGTTGAGCGGAATTTTCTTCTATGGAGGCTCCCAGATCTGCATGATTTGCTGAGGGATGCTGCACAGCTTGTGATAGAGACACTGAGACATAATAGTAGCGATAAGGACGATTGGTCATGCGTGAGAAAAGAAACATTCTCATCTGAGAAAAACGAGTACGCCCACTTGTTAGTTTCAGATTTCTCTTTGTCAGTGCCAACTGCCCCGCCAGAAGTTTTGCAACAATTTTTGGGTGATCCAACGGAGGGCGGCCATGCTCCCGGTAATGTTGCAAATGCTGGCAATCCGGTGGAGTTTGGCCGTGCTCCACGCGATGTTGCAGATCGAAATGCACTTGAAGTATTATTGGAGTCATTTCTACCATGGGCTTATTATGGAGATAGAGACGGTGTTGAGAACGAAAATCAACAAAATGGACATGGTCCAGGCAATGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tcf25

Protein Analysis

626

Amino Acids

70.27

Weight (kDa)

5.56

Isoelectric Point (pI)

42.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tcf25 PF04910 228 - 553 2e-72 Transcriptional repressor TCF25
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 809
Acc65I GGTACC 1 cut(s) 1339
AccB1I GGYRCC 1 cut(s) 1339
AccB7I CCANNNNNTGG 1 cut(s) 1871
AccBSI CCGCTC 2 cut(s) 529, 1447
AccI GTMKAC 1 cut(s) 1268
AccII CGCG 1 cut(s) 1752
AciI CCGC 9 cut(s) 144, 147, 150, 393, 481, 529, 1447, 1646, 1689
AclWI GGATC 5 cut(s) 443, 456, 1064, 1428, 1670
AcoI YGGCCR 2 cut(s) 1690, 1738
AcsI RAATTY 4 cut(s) 409, 650, 1186, 1450
AcuI CTGAAG 3 cut(s) 38, 1167, 1257
AfaI GTAC 5 cut(s) 693, 824, 978, 1341, 1598
AfiI CCNNNNNNNGG 6 cut(s) 344, 345, 923, 1417, 1685, 1871
AhdI GACNNNNNGTC 1 cut(s) 1552
AjiI CACGTC 1 cut(s) 569
AjnI CCWGG 1 cut(s) 1864
AluBI AGCT 9 cut(s) 100, 113, 223, 245, 478, 716, 734, 1277, 1507
AluI AGCT 9 cut(s) 100, 113, 223, 245, 478, 716, 734, 1277, 1507
Alw21I GWGCWC 3 cut(s) 430, 1252, 1747
Alw26I GTCTC 3 cut(s) 1511, 1519, 1818
Alw44I GTGCAC 2 cut(s) 426, 1248
AlwI GGATC 5 cut(s) 443, 456, 1064, 1428, 1670
AlwNI CAGNNNCTG 1 cut(s) 191
AoxI GGCC 3 cut(s) 1080, 1690, 1738
ApaLI GTGCAC 2 cut(s) 426, 1248
ApeKI GCWGC 8 cut(s) 97, 317, 386, 439, 478, 572, 734, 1499
ApoI RAATTY 4 cut(s) 409, 650, 1186, 1450
Asp718I GGTACC 1 cut(s) 1339
AspLEI GCGC 1 cut(s) 1366
AspS9I GGNCC 2 cut(s) 1080, 1862
AsuC2I CCSGG 2 cut(s) 377, 1701
AsuHPI GGTGA 7 cut(s) 92, 130, 668, 851, 1298, 1415, 1685
AsuII TTCGAA 1 cut(s) 1241
AvaII GGWCC 1 cut(s) 1862
BaeGI GKGCMC 2 cut(s) 430, 1252
BamHI GGATCC 1 cut(s) 448
BanI GGYRCC 1 cut(s) 1339
BbsI GAAGAC 2 cut(s) 54, 571
Bbv12I GWGCWC 3 cut(s) 430, 1252, 1747
BbvCI CCTCAGC 2 cut(s) 219, 1490
BbvI GCAGC 8 cut(s) 109, 304, 398, 426, 465, 559, 721, 1486
BccI CCATC 5 cut(s) 338, 626, 662, 1026, 1420
BceAI ACGGC 3 cut(s) 172, 406, 1725
BcgI CGANNNNNNTGC 2 cut(s) 605, 639
BciT130I CCWGG 1 cut(s) 1866
BcnI CCSGG 2 cut(s) 377, 1701
BcoDI GTCTC 3 cut(s) 1511, 1519, 1818
BfaI CTAG 5 cut(s) 546, 585, 1058, 1133, 1876
BglII AGATCT 1 cut(s) 1474
BlpI GCTNAGC 1 cut(s) 417
BmcAI AGTACT 1 cut(s) 824
Bme1390I CCNGG 3 cut(s) 377, 1701, 1866
Bme18I GGWCC 1 cut(s) 1862
BmeRI GACNNNNNGTC 1 cut(s) 1552
BmgBI CACGTC 1 cut(s) 569
BmgT120I GGNCC 2 cut(s) 1080, 1862
BmiI GGNNCC 4 cut(s) 450, 1081, 1341, 1469
BmrFI CCNGG 3 cut(s) 377, 1701, 1866
BmsI GCATC 4 cut(s) 361, 617, 631, 1486
BpiI GAAGAC 2 cut(s) 54, 571
Bpu10I CCTNAGC 2 cut(s) 219, 1490
Bpu1102I GCTNAGC 1 cut(s) 417
Bpu14I TTCGAA 1 cut(s) 1241
BpuEI CTTGAG 1 cut(s) 1244
BpuMI CCSGG 2 cut(s) 377, 1701
Bsa29I ATCGAT 1 cut(s) 1021
BsaAI YACGTR 1 cut(s) 691
BsaBI GATNNNNATC 1 cut(s) 672
BsaJI CCNNGG 7 cut(s) 204, 339, 375, 645, 813, 1416, 1802
BsaWI WCCGGW 1 cut(s) 1726
Bsc4I CCNNNNNNNGG 6 cut(s) 344, 345, 923, 1417, 1685, 1871
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 2 cut(s) 555, 1257
Bse3DI GCAATG 1 cut(s) 1876
Bse8I GATNNNNATC 1 cut(s) 672
BseBI CCWGG 1 cut(s) 1866
BseCI ATCGAT 1 cut(s) 1021
BseDI CCNNGG 7 cut(s) 204, 339, 375, 645, 813, 1416, 1802
BseGI GGATG 7 cut(s) 184, 349, 376, 637, 701, 1412, 1501
BseJI GATNNNNATC 1 cut(s) 672
BseLI CCNNNNNNNGG 6 cut(s) 344, 345, 923, 1417, 1685, 1871
BseMI GCAATG 1 cut(s) 1876
BseMII CTCAG 5 cut(s) 233, 731, 1481, 1514, 1575
BseNI ACTGG 2 cut(s) 555, 1257
BseRI GAGGAG 2 cut(s) 125, 1133
BseSI GKGCMC 2 cut(s) 430, 1252
BseXI GCAGC 8 cut(s) 109, 304, 398, 426, 465, 559, 721, 1486
BseYI CCCAGC 1 cut(s) 627
BsgI GTGCAG 1 cut(s) 1485
Bsh1236I CGCG 1 cut(s) 1752
BshFI GGCC 3 cut(s) 1082, 1692, 1740
BshNI GGYRCC 1 cut(s) 1339
BshVI ATCGAT 1 cut(s) 1021
BsiHKAI GWGCWC 3 cut(s) 430, 1252, 1747
BsiSI CCGG 4 cut(s) 141, 377, 1701, 1727
BslI CCNNNNNNNGG 6 cut(s) 344, 345, 923, 1417, 1685, 1871
BsmAI GTCTC 3 cut(s) 1511, 1519, 1818
BsmBI CGTCTC 1 cut(s) 1818
BsmI GAATGC 1 cut(s) 18
BsnI GGCC 3 cut(s) 1082, 1692, 1740
Bsp119I TTCGAA 1 cut(s) 1241
Bsp1286I GDGCHC 3 cut(s) 430, 1252, 1747
Bsp1720I GCTNAGC 1 cut(s) 417
Bsp19I CCATGG 3 cut(s) 645, 813, 1802
BspACI CCGC 9 cut(s) 144, 147, 150, 393, 481, 529, 1447, 1646, 1689
BspANI GGCC 3 cut(s) 1082, 1692, 1740
BspCNI CTCAG 5 cut(s) 232, 730, 1482, 1515, 1576
BspDI ATCGAT 1 cut(s) 1021
BspFNI CGCG 1 cut(s) 1752
BspLI GGNNCC 4 cut(s) 450, 1081, 1341, 1469
BspPI GGATC 5 cut(s) 443, 456, 1064, 1428, 1670
BspT104I TTCGAA 1 cut(s) 1241
BspT107I GGYRCC 1 cut(s) 1339
BsrBI CCGCTC 2 cut(s) 529, 1447
BsrDI GCAATG 1 cut(s) 1876
BsrFI RCCGGY 1 cut(s) 140
BsrI ACTGG 2 cut(s) 555, 1257
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 7 cut(s) 204, 339, 375, 645, 813, 1416, 1802
BssT1I CCWWGG 5 cut(s) 339, 645, 813, 1416, 1802
Bst2UI CCWGG 1 cut(s) 1866
Bst4CI ACNGT 4 cut(s) 193, 976, 1140, 1829
Bst6I CTCTTC 1 cut(s) 313
BstAPI GCANNNNNTGC 1 cut(s) 1486
BstBAI YACGTR 1 cut(s) 691
BstBI TTCGAA 1 cut(s) 1241
BstC8I GCNNGC 2 cut(s) 391, 1720
BstDEI CTNAG 7 cut(s) 219, 417, 498, 717, 1490, 1523, 1584
BstDSI CCRYGG 4 cut(s) 204, 645, 813, 1802
BstF5I GGATG 7 cut(s) 184, 349, 376, 637, 701, 1412, 1501
BstFNI CGCG 1 cut(s) 1752
BstHHI GCGC 1 cut(s) 1366
BstMAI GTCTC 3 cut(s) 1511, 1519, 1818
BstMWI GCNNNNNNNGC 7 cut(s) 150, 156, 242, 722, 731, 740, 1486
BstNI CCWGG 1 cut(s) 1866
BstSCI CCNGG 3 cut(s) 375, 1699, 1864
BstSLI GKGCMC 2 cut(s) 430, 1252
BstSNI TACGTA 1 cut(s) 691
BstUI CGCG 1 cut(s) 1752
BstV1I GCAGC 8 cut(s) 109, 304, 398, 426, 465, 559, 721, 1486
BstV2I GAAGAC 2 cut(s) 54, 571
BstX2I RGATCY 2 cut(s) 448, 1474
BstYI RGATCY 2 cut(s) 448, 1474
Bsu15I ATCGAT 1 cut(s) 1021
BsuRI GGCC 3 cut(s) 1082, 1692, 1740
BsuTUI ATCGAT 1 cut(s) 1021
BtgI CCRYGG 4 cut(s) 204, 645, 813, 1802
BtgZI GCGATG 2 cut(s) 608, 1767
BtrI CACGTC 1 cut(s) 569
BtsCI GGATG 7 cut(s) 184, 349, 376, 637, 701, 1412, 1501
BtsI GCAGTG 1 cut(s) 603
BtsIMutI CAGTG 4 cut(s) 603, 1250, 1520, 1638
Cac8I GCNNGC 2 cut(s) 391, 1720
CaiI CAGNNNCTG 1 cut(s) 191
CfoI GCGC 1 cut(s) 1366
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 2 cut(s) 1080, 1862
ClaI ATCGAT 1 cut(s) 1021
Csp6I GTAC 5 cut(s) 692, 823, 977, 1340, 1597
CviQI GTAC 5 cut(s) 692, 823, 977, 1340, 1597
DdeI CTNAG 7 cut(s) 219, 417, 498, 717, 1490, 1523, 1584
DraI TTTAAA 1 cut(s) 462
DriI GACNNNNNGTC 1 cut(s) 1552
EaeI YGGCCR 2 cut(s) 1690, 1738
Eam1104I CTCTTC 1 cut(s) 313
Eam1105I GACNNNNNGTC 1 cut(s) 1552
EarI CTCTTC 1 cut(s) 313
Eco105I TACGTA 1 cut(s) 691
Eco130I CCWWGG 5 cut(s) 339, 645, 813, 1416, 1802
Eco32I GATATC 2 cut(s) 674, 754
Eco47I GGWCC 1 cut(s) 1862
Eco57I CTGAAG 3 cut(s) 38, 1167, 1257
EcoRII CCWGG 1 cut(s) 1864
EcoRV GATATC 2 cut(s) 674, 754
EcoT14I CCWWGG 5 cut(s) 339, 645, 813, 1416, 1802
EcoT22I ATGCAT 1 cut(s) 1129
ErhI CCWWGG 5 cut(s) 339, 645, 813, 1416, 1802
Esp3I CGTCTC 1 cut(s) 1818
FauI CCCGC 1 cut(s) 1653
FblI GTMKAC 1 cut(s) 1268
FokI GGATG 7 cut(s) 191, 356, 383, 644, 688, 1399, 1508
FspBI CTAG 5 cut(s) 546, 585, 1058, 1133, 1876
GlaI GCGC 1 cut(s) 1365
GsaI CCCAGC 1 cut(s) 631
HaeIII GGCC 3 cut(s) 1082, 1692, 1740
HapII CCGG 4 cut(s) 141, 377, 1701, 1727
HhaI GCGC 1 cut(s) 1366
Hin6I GCGC 1 cut(s) 1364
HinP1I GCGC 1 cut(s) 1364
HpaII CCGG 4 cut(s) 141, 377, 1701, 1727
HphI GGTGA 7 cut(s) 92, 130, 668, 851, 1298, 1415, 1685
Hpy166II GTNNAC 6 cut(s) 428, 842, 912, 972, 1250, 1269
Hpy188I TCNGA 8 cut(s) 70, 76, 187, 613, 720, 1147, 1585, 1618
Hpy188III TCNNGA 7 cut(s) 173, 353, 546, 656, 868, 1223, 1318
Hpy8I GTNNAC 6 cut(s) 428, 842, 912, 972, 1250, 1269
Hpy99I CGWCG 4 cut(s) 158, 164, 167, 170
HpyAV CCTTC 6 cut(s) 25, 71, 136, 226, 719, 1342
HpyCH4III ACNGT 4 cut(s) 193, 976, 1140, 1829
HpyCH4IV ACGT 2 cut(s) 568, 690
HpyF10VI GCNNNNNNNGC 7 cut(s) 150, 156, 242, 722, 731, 740, 1486
HpyF3I CTNAG 7 cut(s) 219, 417, 498, 717, 1490, 1523, 1584
HpySE526I ACGT 2 cut(s) 568, 690
HspAI GCGC 1 cut(s) 1364
KpnI GGTACC 1 cut(s) 1343
LmnI GCTCC 3 cut(s) 1473, 1702, 1750
Lsp1109I GCAGC 8 cut(s) 109, 304, 398, 426, 465, 559, 721, 1486
LweI GCATC 4 cut(s) 361, 617, 631, 1486
MaeI CTAG 5 cut(s) 546, 585, 1058, 1133, 1876
MaeII ACGT 2 cut(s) 568, 690
MaeIII GTNAC 2 cut(s) 601, 1047
MbiI CCGCTC 2 cut(s) 529, 1447
MfeI CAATTG 1 cut(s) 928
MflI RGATCY 2 cut(s) 448, 1474
MhlI GDGCHC 3 cut(s) 430, 1252, 1747
MluCI AATT 9 cut(s) 360, 409, 650, 762, 928, 1186, 1325, 1450, 1664
MlyI GAGTC 1 cut(s) 1799
MmeI TCCRAC 1 cut(s) 1703
Mph1103I ATGCAT 1 cut(s) 1129
MseI TTAA 1 cut(s) 461
MspA1I CMGCKG 3 cut(s) 223, 393, 1277
MspI CCGG 4 cut(s) 141, 377, 1701, 1727
MspR9I CCNGG 3 cut(s) 377, 1701, 1866
MunI CAATTG 1 cut(s) 928
Mva1269I GAATGC 1 cut(s) 18
MvaI CCWGG 1 cut(s) 1866
MvnI CGCG 1 cut(s) 1752
MwoI GCNNNNNNNGC 7 cut(s) 150, 156, 242, 722, 731, 740, 1486
NciI CCSGG 2 cut(s) 377, 1701
NcoI CCATGG 3 cut(s) 645, 813, 1802
NlaIV GGNNCC 4 cut(s) 450, 1081, 1341, 1469
NmuCI GTSAC 1 cut(s) 601
NsiI ATGCAT 1 cut(s) 1129
NspV TTCGAA 1 cut(s) 1241
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PctI GAATGC 1 cut(s) 18
PflFI GACNNNGTC 1 cut(s) 1860
PflMI CCANNNNNTGG 1 cut(s) 1871
PleI GAGTC 1 cut(s) 1798
PpsI GAGTC 1 cut(s) 1798
Ppu21I YACGTR 1 cut(s) 691
PsiI TTATAA 1 cut(s) 809
Psp6I CCWGG 1 cut(s) 1864
PspFI CCCAGC 1 cut(s) 627
PspGI CCWGG 1 cut(s) 1864
PspN4I GGNNCC 4 cut(s) 450, 1081, 1341, 1469
PspPI GGNCC 2 cut(s) 1080, 1862
PstNI CAGNNNCTG 1 cut(s) 191
PsuI RGATCY 2 cut(s) 448, 1474
PsyI GACNNNGTC 1 cut(s) 1860
PvuII CAGCTG 2 cut(s) 223, 1277
RsaI GTAC 5 cut(s) 693, 824, 978, 1341, 1598
RsaNI GTAC 5 cut(s) 692, 823, 977, 1340, 1597
SaqAI TTAA 1 cut(s) 461
Sau96I GGNCC 2 cut(s) 1080, 1862
ScaI AGTACT 1 cut(s) 824
SchI GAGTC 1 cut(s) 1799
ScrFI CCNGG 3 cut(s) 377, 1701, 1866
SduI GDGCHC 3 cut(s) 430, 1252, 1747
SfaNI GCATC 4 cut(s) 361, 617, 631, 1486
SfuI TTCGAA 1 cut(s) 1241
SgrAI CRCCGGYG 1 cut(s) 140
SinI GGWCC 1 cut(s) 1862
SmiI ATTTAAAT 1 cut(s) 462
SmlI CTYRAG 1 cut(s) 1223
SmoI CTYRAG 1 cut(s) 1223
SnaBI TACGTA 1 cut(s) 691
Sse9I AATT 9 cut(s) 360, 409, 650, 762, 928, 1186, 1325, 1450, 1664
SsiI CCGC 9 cut(s) 144, 147, 150, 393, 481, 529, 1447, 1646, 1689
SspMI CTAG 5 cut(s) 546, 585, 1058, 1133, 1876
StyD4I CCNGG 3 cut(s) 375, 1699, 1864
StyI CCWWGG 5 cut(s) 339, 645, 813, 1416, 1802
SwaI ATTTAAAT 1 cut(s) 462
TaaI ACNGT 4 cut(s) 193, 976, 1140, 1829
TaiI ACGT 2 cut(s) 571, 693
TaqI TCGA 8 cut(s) 10, 129, 330, 685, 869, 1021, 1241, 1765
TaqII GACCGA 1 cut(s) 66
TasI AATT 9 cut(s) 360, 409, 650, 762, 928, 1186, 1325, 1450, 1664
TatI WGTACW 1 cut(s) 822
TauI GCSGC 4 cut(s) 147, 150, 153, 1692
Tru1I TTAA 1 cut(s) 461
Tru9I TTAA 1 cut(s) 461
TscAI CASTG 4 cut(s) 610, 1257, 1527, 1638
TseFI GTSAC 1 cut(s) 601
TseI GCWGC 8 cut(s) 97, 317, 386, 439, 478, 572, 734, 1499
Tsp45I GTSAC 1 cut(s) 601
TspDTI ATGAA 5 cut(s) 195, 213, 966, 1182, 1301
TspGWI ACGGA 1 cut(s) 1697
TspRI CASTG 4 cut(s) 610, 1257, 1527, 1638
Tth111I GACNNNGTC 1 cut(s) 1860
Van91I CCANNNNNTGG 1 cut(s) 1871
VneI GTGCAC 2 cut(s) 426, 1248
VpaK11BI GGWCC 1 cut(s) 1862
XapI RAATTY 4 cut(s) 409, 650, 1186, 1450
XbaI TCTAGA 1 cut(s) 545
XcmI CCANNNNNNNNNTGG 1 cut(s) 101
XmiI GTMKAC 1 cut(s) 1268
XspI CTAG 5 cut(s) 546, 585, 1058, 1133, 1876
ZrmI AGTACT 1 cut(s) 824
Zsp2I ATGCAT 1 cut(s) 1129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.