RLG00000027697

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0007094
Physical Location & Seq
Reverse (-)
14096110 .. 14099300
3191 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027697

Sequence Viewer

Length: 1881 bp
ATGTCTGCTCGGTATCTGAAGAAAGTGTTGAAGGAAGAACAAGAGGAGCGACAATTGGAATCCGACCACGAAGAAGCAGAAGACGACGACCACCGCCAGCTCAACGGCAAAGCAAAGATCAACCCTTTCGACCTCCTCAACGACGACGACAACGACGCCGACGACCCCCAGGAGGATGAGTCAGAAACTGCTGTTGATGAACCCTCGAGTGTGGTGAAGCCTAGTGCTGATGTGGTTTCGACATCCAGTCAAAAATCGAAGAAGAAGAAGAAGAAGAAAGGCAAAGAGGGCTCTTCTTCTAATGCAGCTGCAGTGAGAGTTAAAAAGTCGCCGGATGAGGTTCTTGAAGAATTCTATGACTATGCGCATGAAAGACAAGCCGAGACGAGGGCTACTGGTGATGCGGTTAAGCATTCGTATGAAAAGTATGTGCCCTTCATATTGCAAGTGGATCCCAAGTACTTGAATGCCGACAATGAGATGCGAAGAATCTTTGGGGCTAAGGTGGTCAAGTCGTTTGAGAAGAATCAACAGAGTGGGAGCTCTAGAATGGCGCGTGGTGGAAGACGTGGCGCGGTTCATAGGAAGTCTTTCCTTGTCACTCCGTTGGAGCATTGGCCTCGCTGGGATGGTGCTATGAACATGGAACTCCTTCACTCGCCCAATGGATACTATGATTATCGATATACGTATTCGTCAGCCTACCGTCAAGCTCAGAGTGCATTTGAATCTGCCAAAGCTATACATGATCTCAATGCCATTGCAAGTGTTTTATTATACTATCCTTACCATTTGGATTCACTACTAACCATGGCAGAGTACTTTAAGTTTGTGGGTGAGCATCAAATGTCAGCAGATGCTATCTCGAAGTGCTTATATGCTTTGGAATGTGCATGGTCTCCAATGTTCACCCCCTTTAAGGGTGATTGCCAAGTGAAATACAGCCATGAAGCAAACAAACCTCTGTTTGTTGCACTTTTTACTCACATGAAAAGTATGGACAGACGTGGCTGTCATCGATCTGCTTTAGAAGTTTGCAAGTTTTTACTGTCACTAGATTCTGACGATCCAATGGGGGCCATGTTCTGTATTGACTATTTTGCGGTGAGGGCAGAGGAGTATGCATGGCTAGAACGTTTCTCTGAAGAATATAAAAGTGATAACACCCTATGGTTGTTTCCAAATTTTTCATATTCCCTTGCCGTATGCCGATTGTATCTTGAGAAAGCGGAATCTTCGAAAGATCCAGCTATGGAAACCACCAAGGCTACTTCGACTGATCTTATGAAGCAGGCATTGATGCTTCATCCCACAGTCCTCAAGAAACTAGTGGCAAAGGTACCTTTGAAGGAACGGGTGTGGACGGATATACTCAAAAATGCATTCTTCCAAGCAGATGAAGTAGGAATCCCATCCTTGGACCACCTGATTAACATATACGTTGAGAGTAATCATATTATATGGAGGCTTCCAGAATTGCAGAAATTGCTGCGGGAAGCAGCACAGCTGGTGATCGAGACTCTAAGACAGAATAGTAGCGAAAAGAATGATTGGGCATGTGTGAGAAAAGAAGCATTCTCATCTGACAAGAATGAGTATTCCCACTTGTTGGTTTCAGATTTCTCTTATACATTGCCTGCTGCCCCACCTGAAAATTTGCAACATTTTATGGCCAATGCAAGGATGGAGGATGCTGTGCATAATGAGCAGCATCTTGTCAATCTAAATGACGGTGGCCATGCTCCCCGTGATGTTGCAAATCGAAATCCATTAGCGGTCTTGTTTGAGTCGGTGTTACCATGGGTTCATTATGGGGAAGGAGCAGTTGTTGAGGAGGAAGACCAACAAAATGGCCATGGTCCGGGCAATCAAGAACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tcf25

Protein Analysis

627

Amino Acids

71.53

Weight (kDa)

5.6

Isoelectric Point (pI)

43.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tcf25 PF04910 227 - 551 6.5e-69 Transcriptional repressor TCF25
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1011
Acc16I TGCGCA 1 cut(s) 366
Acc65I GGTACC 1 cut(s) 1339
AccB1I GGYRCC 1 cut(s) 1339
AccB7I CCANNNNNTGG 1 cut(s) 1609
AccII CGCG 2 cut(s) 556, 575
AciI CCGC 7 cut(s) 94, 404, 575, 1103, 1229, 1492, 1775
AclI AACGTT 2 cut(s) 1135, 1876
AclWI GGATC 4 cut(s) 446, 459, 1061, 1238
AcoI YGGCCR 3 cut(s) 1671, 1735, 1852
AcsI RAATTY 3 cut(s) 350, 1183, 1654
AcuI CTGAAG 2 cut(s) 38, 1164
AcyI GRCGYC 1 cut(s) 156
AfaI GTAC 3 cut(s) 461, 821, 1341
AfiI CCNNNNNNNGG 8 cut(s) 172, 387, 919, 920, 1417, 1609, 1680, 1861
AgsI TTSAA 5 cut(s) 31, 347, 466, 728, 1348
AhdI GACNNNNNGTC 1 cut(s) 246
AhlI ACTAGT 1 cut(s) 1327
AjiI CACGTC 2 cut(s) 569, 1007
AjnI CCWGG 1 cut(s) 168
AluBI AGCT 7 cut(s) 100, 308, 543, 713, 740, 1250, 1507
AluI AGCT 7 cut(s) 100, 308, 543, 713, 740, 1250, 1507
Alw21I GWGCWC 1 cut(s) 545
Alw26I GTCTC 3 cut(s) 377, 903, 1511
AlwI GGATC 4 cut(s) 446, 459, 1061, 1238
AlwNI CAGNNNCTG 1 cut(s) 188
Ama87I CYCGRG 1 cut(s) 205
AoxI GGCC 5 cut(s) 617, 1077, 1671, 1735, 1852
ApeKI GCWGC 6 cut(s) 305, 308, 1489, 1499, 1640, 1708
ApoI RAATTY 3 cut(s) 350, 1183, 1654
Asp700I GAANNNNTTC 2 cut(s) 590, 651
Asp718I GGTACC 1 cut(s) 1339
AspLEI GCGC 3 cut(s) 367, 556, 575
AspS9I GGNCC 3 cut(s) 1077, 1420, 1859
AsuC2I CCSGG 1 cut(s) 1863
AsuHPI GGTGA 7 cut(s) 226, 410, 848, 901, 935, 1117, 1522
AsuII TTCGAA 1 cut(s) 1238
AvaI CYCGRG 1 cut(s) 205
AvaII GGWCC 2 cut(s) 1420, 1859
BaeGI GKGCMC 1 cut(s) 435
BalI TGGCCA 3 cut(s) 1673, 1737, 1854
BamHI GGATCC 1 cut(s) 451
BanI GGYRCC 1 cut(s) 1339
BanII GRGCYC 2 cut(s) 293, 545
BarI GAAGNNNNNNTAC 2 cut(s) 419, 451
BbsI GAAGAC 3 cut(s) 87, 571, 1845
Bbv12I GWGCWC 1 cut(s) 545
BbvI GCAGC 6 cut(s) 295, 317, 1476, 1511, 1627, 1720
BccI CCATC 3 cut(s) 623, 1420, 1678
BceAI ACGGC 2 cut(s) 121, 1187
BcgI CGANNNNNNTGC 2 cut(s) 602, 636
BciT130I CCWGG 1 cut(s) 170
BciVI GTATCC 1 cut(s) 662
BcnI CCSGG 1 cut(s) 1863
BcoDI GTCTC 3 cut(s) 377, 903, 1511
BcuI ACTAGT 1 cut(s) 1327
BfaI CTAG 5 cut(s) 222, 546, 1055, 1130, 1328
BfmI CTRYAG 1 cut(s) 309
BfuI GTATCC 1 cut(s) 662
BisI GCNGC 6 cut(s) 306, 309, 1490, 1500, 1641, 1709
BlsI GCNGC 6 cut(s) 307, 310, 1491, 1501, 1642, 1710
BmcAI AGTACT 2 cut(s) 461, 821
Bme1390I CCNGG 2 cut(s) 170, 1863
Bme18I GGWCC 2 cut(s) 1420, 1859
BmeRI GACNNNNNGTC 1 cut(s) 246
BmeT110I CYCGRG 1 cut(s) 205
BmgBI CACGTC 2 cut(s) 569, 1007
BmgT120I GGNCC 3 cut(s) 1077, 1420, 1859
BmiI GGNNCC 3 cut(s) 453, 1078, 1341
BmrFI CCNGG 2 cut(s) 170, 1863
BmsI GCATC 7 cut(s) 391, 471, 847, 850, 1290, 1681, 1720
BpiI GAAGAC 3 cut(s) 87, 571, 1845
Bpu10I CCTNAGC 1 cut(s) 501
Bpu14I TTCGAA 1 cut(s) 1238
BpuEI CTTGAG 2 cut(s) 1241, 1304
BpuMI CCSGG 1 cut(s) 1863
Bsa29I ATCGAT 2 cut(s) 682, 1018
BsaAI YACGTR 1 cut(s) 690
BsaHI GRCGYC 1 cut(s) 156
BsaI GGTCTC 1 cut(s) 903
BsaJI CCNNGG 6 cut(s) 168, 810, 1263, 1416, 1799, 1855
BsaXI ACNNNNNCTCC 4 cut(s) 164, 194, 1812, 1842
Bsc4I CCNNNNNNNGG 8 cut(s) 172, 387, 919, 920, 1417, 1609, 1680, 1861
Bse1I ACTGG 2 cut(s) 246, 400
Bse3DI GCAATG 2 cut(s) 759, 1631
BseBI CCWGG 1 cut(s) 170
BseCI ATCGAT 2 cut(s) 682, 1018
BseDI CCNNGG 6 cut(s) 168, 810, 1263, 1416, 1799, 1855
BseGI GGATG 8 cut(s) 181, 242, 340, 634, 1306, 1412, 1689, 1696
BseLI CCNNNNNNNGG 8 cut(s) 172, 387, 919, 920, 1417, 1609, 1680, 1861
BseMI GCAATG 2 cut(s) 759, 1631
BseMII CTCAG 1 cut(s) 728
BseNI ACTGG 2 cut(s) 246, 400
BseRI GAGGAG 4 cut(s) 59, 125, 1130, 1847
BseSI GKGCMC 1 cut(s) 435
BseXI GCAGC 6 cut(s) 295, 317, 1476, 1511, 1627, 1720
BseYI CCCAGC 1 cut(s) 624
Bsh1236I CGCG 2 cut(s) 556, 575
BshFI GGCC 5 cut(s) 619, 1079, 1673, 1737, 1854
BshNI GGYRCC 1 cut(s) 1339
BshVI ATCGAT 2 cut(s) 682, 1018
BsiHKAI GWGCWC 1 cut(s) 545
BsiHKCI CYCGRG 1 cut(s) 205
BsiSI CCGG 2 cut(s) 332, 1862
BslI CCNNNNNNNGG 8 cut(s) 172, 387, 919, 920, 1417, 1609, 1680, 1861
BsmAI GTCTC 3 cut(s) 377, 903, 1511
BsmBI CGTCTC 1 cut(s) 377
BsmI GAATGC 4 cut(s) 412, 472, 1382, 1574
BsnI GGCC 5 cut(s) 619, 1079, 1673, 1737, 1854
Bso31I GGTCTC 1 cut(s) 903
BsoBI CYCGRG 1 cut(s) 205
Bsp119I TTCGAA 1 cut(s) 1238
Bsp1286I GDGCHC 3 cut(s) 293, 435, 545
Bsp143I GATC 8 cut(s) 117, 451, 748, 1019, 1066, 1243, 1279, 1512
Bsp19I CCATGG 3 cut(s) 810, 1799, 1855
BspACI CCGC 7 cut(s) 94, 404, 575, 1103, 1229, 1492, 1775
BspANI GGCC 5 cut(s) 619, 1079, 1673, 1737, 1854
BspCNI CTCAG 1 cut(s) 727
BspDI ATCGAT 2 cut(s) 682, 1018
BspFNI CGCG 2 cut(s) 556, 575
BspLI GGNNCC 3 cut(s) 453, 1078, 1341
BspMAI CTGCAG 1 cut(s) 313
BspPI GGATC 4 cut(s) 446, 459, 1061, 1238
BspQI GCTCTTC 1 cut(s) 298
BspT104I TTCGAA 1 cut(s) 1238
BspT107I GGYRCC 1 cut(s) 1339
BspTNI GGTCTC 1 cut(s) 903
BsrDI GCAATG 2 cut(s) 759, 1631
BsrI ACTGG 2 cut(s) 246, 400
BssECI CCNNGG 6 cut(s) 168, 810, 1263, 1416, 1799, 1855
BssMI GATC 8 cut(s) 117, 451, 748, 1019, 1066, 1243, 1279, 1512
BssNI GRCGYC 1 cut(s) 156
BssT1I CCWWGG 5 cut(s) 810, 1263, 1416, 1799, 1855
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 4 cut(s) 707, 1050, 1315, 1733
Bst6I CTCTTC 1 cut(s) 298
BstACI GRCGYC 1 cut(s) 156
BstAPI GCANNNNNTGC 1 cut(s) 1486
BstBAI YACGTR 1 cut(s) 690
BstBI TTCGAA 1 cut(s) 1238
BstC8I GCNNGC 3 cut(s) 98, 1293, 1638
BstDEI CTNAG 3 cut(s) 501, 714, 1523
BstDSI CCRYGG 3 cut(s) 810, 1799, 1855
BstF5I GGATG 8 cut(s) 181, 242, 340, 634, 1306, 1412, 1689, 1696
BstFNI CGCG 2 cut(s) 556, 575
BstHHI GCGC 3 cut(s) 367, 556, 575
BstKTI GATC 8 cut(s) 120, 454, 751, 1022, 1069, 1246, 1282, 1515
BstMAI GTCTC 3 cut(s) 377, 903, 1511
BstMBI GATC 8 cut(s) 117, 451, 748, 1019, 1066, 1243, 1279, 1512
BstMWI GCNNNNNNNGC 5 cut(s) 288, 719, 1109, 1486, 1705
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 1560
BstSCI CCNGG 2 cut(s) 168, 1861
BstSFI CTRYAG 1 cut(s) 309
BstSLI GKGCMC 1 cut(s) 435
BstSNI TACGTA 1 cut(s) 690
BstUI CGCG 2 cut(s) 556, 575
BstV1I GCAGC 6 cut(s) 295, 317, 1476, 1511, 1627, 1720
BstV2I GAAGAC 3 cut(s) 87, 571, 1845
BstX2I RGATCY 2 cut(s) 451, 1243
BstXI CCANNNNNNTGG 1 cut(s) 1850
BstYI RGATCY 2 cut(s) 451, 1243
Bsu15I ATCGAT 2 cut(s) 682, 1018
BsuI GTATCC 1 cut(s) 662
BsuRI GGCC 5 cut(s) 619, 1079, 1673, 1737, 1854
BsuTUI ATCGAT 2 cut(s) 682, 1018
BtgI CCRYGG 3 cut(s) 810, 1799, 1855
BtrI CACGTC 2 cut(s) 569, 1007
BtsCI GGATG 8 cut(s) 181, 242, 340, 634, 1306, 1412, 1689, 1696
BtsI GCAGTG 1 cut(s) 318
BtsIMutI CAGTG 1 cut(s) 318
Cac8I GCNNGC 3 cut(s) 98, 1293, 1638
CaiI CAGNNNCTG 1 cut(s) 188
CfoI GCGC 3 cut(s) 367, 556, 575
Cfr13I GGNCC 3 cut(s) 1077, 1420, 1859
ClaI ATCGAT 2 cut(s) 682, 1018
CseI GACGC 1 cut(s) 164
Csp6I GTAC 3 cut(s) 460, 820, 1340
CviQI GTAC 3 cut(s) 460, 820, 1340
DdeI CTNAG 3 cut(s) 501, 714, 1523
DpnI GATC 8 cut(s) 119, 453, 750, 1021, 1068, 1245, 1281, 1514
DpnII GATC 8 cut(s) 117, 451, 748, 1019, 1066, 1243, 1279, 1512
DrdI GACNNNNNNGTC 1 cut(s) 1011
DriI GACNNNNNGTC 1 cut(s) 246
DseDI GACNNNNNNGTC 1 cut(s) 1011
EaeI YGGCCR 3 cut(s) 1671, 1735, 1852
Eam1104I CTCTTC 1 cut(s) 298
Eam1105I GACNNNNNGTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 298
Ecl136II GAGCTC 1 cut(s) 543
Eco105I TACGTA 1 cut(s) 690
Eco130I CCWWGG 5 cut(s) 810, 1263, 1416, 1799, 1855
Eco24I GRGCYC 2 cut(s) 293, 545
Eco31I GGTCTC 1 cut(s) 903
Eco47I GGWCC 2 cut(s) 1420, 1859
Eco53kI GAGCTC 1 cut(s) 543
Eco57I CTGAAG 2 cut(s) 38, 1164
Eco88I CYCGRG 1 cut(s) 205
EcoICRI GAGCTC 1 cut(s) 543
EcoRI GAATTC 1 cut(s) 350
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 5 cut(s) 810, 1263, 1416, 1799, 1855
EcoT22I ATGCAT 2 cut(s) 1126, 1384
EcoT38I GRGCYC 2 cut(s) 293, 545
ErhI CCWWGG 5 cut(s) 810, 1263, 1416, 1799, 1855
Esp3I CGTCTC 1 cut(s) 377
FauI CCCGC 1 cut(s) 1485
Fnu4HI GCNGC 6 cut(s) 306, 309, 1490, 1500, 1641, 1709
FokI GGATG 8 cut(s) 188, 229, 347, 641, 1293, 1399, 1696, 1703
FriOI GRGCYC 2 cut(s) 293, 545
Fsp4HI GCNGC 6 cut(s) 306, 309, 1490, 1500, 1641, 1709
FspAI RTGCGCAY 1 cut(s) 366
FspBI CTAG 5 cut(s) 222, 546, 1055, 1130, 1328
FspI TGCGCA 1 cut(s) 366
GlaI GCGC 3 cut(s) 366, 555, 574
GluI GCNGC 6 cut(s) 306, 309, 1490, 1500, 1641, 1709
GsaI CCCAGC 1 cut(s) 628
HaeIII GGCC 5 cut(s) 619, 1079, 1673, 1737, 1854
HapII CCGG 2 cut(s) 332, 1862
HgaI GACGC 1 cut(s) 164
HhaI GCGC 3 cut(s) 367, 556, 575
Hin1I GRCGYC 1 cut(s) 156
Hin6I GCGC 3 cut(s) 365, 554, 573
HinP1I GCGC 3 cut(s) 365, 554, 573
HpaII CCGG 2 cut(s) 332, 1862
HphI GGTGA 7 cut(s) 226, 410, 848, 901, 935, 1117, 1522
Hpy166II GTNNAC 2 cut(s) 909, 1362
Hpy188I TCNGA 8 cut(s) 18, 64, 184, 717, 1063, 1144, 1585, 1618
Hpy188III TCNNGA 8 cut(s) 344, 546, 865, 1220, 1321, 1472, 1516, 1871
Hpy8I GTNNAC 2 cut(s) 909, 1362
Hpy99I CGWCG 5 cut(s) 89, 146, 149, 158, 164
HpyAV CCTTC 5 cut(s) 25, 445, 662, 1342, 1811
HpyCH4III ACNGT 4 cut(s) 707, 1050, 1315, 1733
HpyCH4IV ACGT 6 cut(s) 568, 689, 1006, 1135, 1440, 1876
HpyF10VI GCNNNNNNNGC 5 cut(s) 288, 719, 1109, 1486, 1705
HpyF3I CTNAG 3 cut(s) 501, 714, 1523
HpySE526I ACGT 6 cut(s) 568, 689, 1006, 1135, 1440, 1876
Hsp92I GRCGYC 1 cut(s) 156
HspAI GCGC 3 cut(s) 365, 554, 573
KpnI GGTACC 1 cut(s) 1343
Kzo9I GATC 8 cut(s) 117, 451, 748, 1019, 1066, 1243, 1279, 1512
LguI GCTCTTC 1 cut(s) 298
LmnI GCTCC 5 cut(s) 46, 540, 610, 1747, 1820
Lsp1109I GCAGC 6 cut(s) 295, 317, 1476, 1511, 1627, 1720
LweI GCATC 7 cut(s) 391, 471, 847, 850, 1290, 1681, 1720
MaeI CTAG 5 cut(s) 222, 546, 1055, 1130, 1328
MaeII ACGT 6 cut(s) 568, 689, 1006, 1135, 1440, 1876
MaeIII GTNAC 3 cut(s) 598, 1050, 1794
MalI GATC 8 cut(s) 119, 453, 750, 1021, 1068, 1245, 1281, 1514
MboI GATC 8 cut(s) 117, 451, 748, 1019, 1066, 1243, 1279, 1512
MfeI CAATTG 1 cut(s) 53
MflI RGATCY 2 cut(s) 451, 1243
MhlI GDGCHC 3 cut(s) 293, 435, 545
MlsI TGGCCA 3 cut(s) 1673, 1737, 1854
MluCI AATT 6 cut(s) 53, 350, 1183, 1475, 1484, 1654
MluNI TGGCCA 3 cut(s) 1673, 1737, 1854
MlyI GAGTC 3 cut(s) 188, 1513, 1796
MmeI TCCRAC 2 cut(s) 87, 588
Mox20I TGGCCA 3 cut(s) 1673, 1737, 1854
Mph1103I ATGCAT 2 cut(s) 1126, 1384
MroXI GAANNNNTTC 2 cut(s) 590, 651
MscI TGGCCA 3 cut(s) 1673, 1737, 1854
MseI TTAA 5 cut(s) 321, 408, 825, 918, 1431
MslI CAYNNNNRTG 1 cut(s) 417
Msp20I TGGCCA 3 cut(s) 1673, 1737, 1854
MspA1I CMGCKG 2 cut(s) 308, 1507
MspI CCGG 2 cut(s) 332, 1862
MspR9I CCNGG 2 cut(s) 170, 1863
MunI CAATTG 1 cut(s) 53
Mva1269I GAATGC 4 cut(s) 412, 472, 1382, 1574
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 2 cut(s) 556, 575
MwoI GCNNNNNNNGC 5 cut(s) 288, 719, 1109, 1486, 1705
NciI CCSGG 1 cut(s) 1863
NcoI CCATGG 3 cut(s) 810, 1799, 1855
NdeII GATC 8 cut(s) 117, 451, 748, 1019, 1066, 1243, 1279, 1512
NlaIV GGNNCC 3 cut(s) 453, 1078, 1341
NmeAIII GCCGAG 1 cut(s) 406
NmuCI GTSAC 2 cut(s) 598, 1050
NsbI TGCGCA 1 cut(s) 366
NsiI ATGCAT 2 cut(s) 1126, 1384
NspI RCATGY 1 cut(s) 1560
NspV TTCGAA 1 cut(s) 1238
PaeR7I CTCGAG 1 cut(s) 205
PciSI GCTCTTC 1 cut(s) 298
PcsI WCGNNNNNNNCGW 2 cut(s) 150, 159
PctI GAATGC 4 cut(s) 412, 472, 1382, 1574
PdmI GAANNNNTTC 2 cut(s) 590, 651
PfeI GAWTC 8 cut(s) 59, 489, 526, 728, 797, 1058, 1232, 1407
PflMI CCANNNNNTGG 1 cut(s) 1609
PkrI GCNGC 6 cut(s) 307, 310, 1491, 1501, 1642, 1710
PleI GAGTC 3 cut(s) 187, 1513, 1795
PpsI GAGTC 3 cut(s) 187, 1513, 1795
Ppu21I YACGTR 1 cut(s) 690
Psp124BI GAGCTC 1 cut(s) 545
Psp1406I AACGTT 2 cut(s) 1135, 1876
Psp6I CCWGG 1 cut(s) 168
PspFI CCCAGC 1 cut(s) 624
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 3 cut(s) 453, 1078, 1341
PspPI GGNCC 3 cut(s) 1077, 1420, 1859
PspXI VCTCGAGB 1 cut(s) 205
PstI CTGCAG 1 cut(s) 313
PstNI CAGNNNCTG 1 cut(s) 188
PsuI RGATCY 2 cut(s) 451, 1243
PvuII CAGCTG 2 cut(s) 308, 1507
RsaI GTAC 3 cut(s) 461, 821, 1341
RsaNI GTAC 3 cut(s) 460, 820, 1340
RseI CAYNNNNRTG 1 cut(s) 417
SacI GAGCTC 1 cut(s) 545
SapI GCTCTTC 1 cut(s) 298
SaqAI TTAA 5 cut(s) 321, 408, 825, 918, 1431
SatI GCNGC 6 cut(s) 306, 309, 1490, 1500, 1641, 1709
Sau3AI GATC 8 cut(s) 117, 451, 748, 1019, 1066, 1243, 1279, 1512
Sau96I GGNCC 3 cut(s) 1077, 1420, 1859
ScaI AGTACT 2 cut(s) 461, 821
SchI GAGTC 3 cut(s) 188, 1513, 1796
ScrFI CCNGG 2 cut(s) 170, 1863
SduI GDGCHC 3 cut(s) 293, 435, 545
SfaNI GCATC 7 cut(s) 391, 471, 847, 850, 1290, 1681, 1720
SfcI CTRYAG 1 cut(s) 309
Sfr274I CTCGAG 1 cut(s) 205
SfuI TTCGAA 1 cut(s) 1238
SinI GGWCC 2 cut(s) 1420, 1859
SlaI CTCGAG 1 cut(s) 205
SmiMI CAYNNNNRTG 1 cut(s) 417
SmlI CTYRAG 3 cut(s) 205, 1220, 1319
SmoI CTYRAG 3 cut(s) 205, 1220, 1319
SnaBI TACGTA 1 cut(s) 690
SpeI ACTAGT 1 cut(s) 1327
Sse9I AATT 6 cut(s) 53, 350, 1183, 1475, 1484, 1654
SsiI CCGC 7 cut(s) 94, 404, 575, 1103, 1229, 1492, 1775
SspMI CTAG 5 cut(s) 222, 546, 1055, 1130, 1328
SstI GAGCTC 1 cut(s) 545
StyD4I CCNGG 2 cut(s) 168, 1861
StyI CCWWGG 5 cut(s) 810, 1263, 1416, 1799, 1855
TaaI ACNGT 4 cut(s) 707, 1050, 1315, 1733
TaiI ACGT 6 cut(s) 571, 692, 1009, 1138, 1443, 1879
TasI AATT 6 cut(s) 53, 350, 1183, 1475, 1484, 1654
TatI WGTACW 2 cut(s) 459, 819
TfiI GAWTC 8 cut(s) 59, 489, 526, 728, 797, 1058, 1232, 1407
Tru1I TTAA 5 cut(s) 321, 408, 825, 918, 1431
Tru9I TTAA 5 cut(s) 321, 408, 825, 918, 1431
TscAI CASTG 1 cut(s) 318
TseFI GTSAC 2 cut(s) 598, 1050
TseI GCWGC 6 cut(s) 305, 308, 1489, 1499, 1640, 1708
Tsp45I GTSAC 2 cut(s) 598, 1050
TspGWI ACGGA 2 cut(s) 594, 1379
TspRI CASTG 1 cut(s) 318
Van91I CCANNNNNTGG 1 cut(s) 1609
VpaK11BI GGWCC 2 cut(s) 1420, 1859
XapI RAATTY 3 cut(s) 350, 1183, 1654
XbaI TCTAGA 1 cut(s) 545
XceI RCATGY 1 cut(s) 1560
XcmI CCANNNNNNNNNTGG 1 cut(s) 1681
XhoI CTCGAG 1 cut(s) 205
XmnI GAANNNNTTC 2 cut(s) 590, 651
XspI CTAG 5 cut(s) 222, 546, 1055, 1130, 1328
ZrmI AGTACT 2 cut(s) 461, 821
Zsp2I ATGCAT 2 cut(s) 1126, 1384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.