MD07G1165200.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007094
Physical Location & Seq
Forward (+)
24014738 .. 24018302
3565 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1165200.v1.1.491

Sequence Viewer

Length: 1878 bp
ATGTCCGGTCGAATGCTGAAGAAAGTTTTGAAGGAACAAGAAGAAGAACACGGACACGTAATAGAATCCGAATCAGAAGGAGAAGAAGAAAGCCAGCAGCTCAACGGAAAAGCTAAATTCAATCCCTTTGATCTCCTCAACGACGACGGGGACGACGACGGCGACCACCAGGGGGATGAATCTGAAACTGTTGATGAACACTTGGTTGTTGTTCCTGAGCTGAAAACGAAGGCCGGTGTAGCGTCAACCAACAATCAGAAATCAAAGAAGAAGAAAAAGAAGAAAAGCAAAGAGGCTTCTTATTCTTCTTCGGGTGCTTCCAATGTAGAAAAACCCTTGGATGTGATTCTTGAGTCAATCTCTTTGGATGGCCCGGAAAAAGCTGCCAGTGGAAAGGGTTGTGAGAATTTGGCTAAGCCGTGTGCGCCCTCTGCGTTACAAGTGGATCCCAAGTACTTAAATGTTGACAATGAGCTGCGGAGAATCTTTGGCTCTAAGGTGGTGAAGTCCTTTGAGAAACATGAGCGAAGTGGTGGCAGTTCTAGAATGGTGCGTGGTGGAAGACGTGCTGCTCATATTCCTAGAAAAACTATACTTGTCACTGCATCAGAGCATTGGCATCGCTGGGATGGTTCTTTGTCCATGGAATTTCTTGGAGTGATTGATGGATACCACCACTTTCGATATGTACATTCCTCATCCTACCTTCAAGCTCAGAGGGCATTTGAAGCTGCTAAAGCTACACATGACATCAATGGAATTGCAAGTATTTTGTTATACAGTCCTTATCATTTGGATTCGCTTATAACCATGGCAGAGTACTATAAGTTTTCGGGTGAACATCAAATATCAGCAGAACTTATCTCGAAGTGCTTGTATGCTATGGAATGTGCATGGAATCCAATGTTCACCCCCTTGCAGGGCAATTGCCAACTAAGATACAGCCATGAAACAAACAAGCCTCTGTTCACTGTACTTTTTACTCACATGAGAAATATGGATAGGCGTGGTTGCCATCGATCGGCTTTAGAAGTTTGCAAGTTGTTACTTTCACTAGATTCTGATGATCCAATGGGGGCCATGTTCTGCATTGACTATTTTTCGTTGAGGGCGGAGGAGTATGCATGGCTAGAACGGTTCTCTGAAGAATATAAAAGTGATAACTCCTTGTGGTTGTTTCCAAATTTTTCATATTCCCTTGCCATGTGCCGTTTGTATCTTGAGAAAGAGGAATCTTCAAAAGGTGCACCGGAAAATGCTAAGTCTACTTCAGCTGATCTTATGAAGCAGGCACTAATGCTTCACCCCTCAGTTCTAAAGAAATTAGTGGAAAAGGTGCCTTTGAAGGACAAGGTGTGGGCACAGATACTTAAAAATCCATATTTTCAAGCAGATCAAGTAGGAATCCCATCCTTGGATCACCTAATAAACATATATGTCGAGAGGAATTTTCTTCTATGGAGGCTTCCAGATCTGCAAGATTTGCTGAGGGATGCTGCACAGCTTGTGATCGAGACACTAAGACATGATAGTAGCGAGAAGAACGATTGGGCATGCGTGAGAAAAGAAGCATTCTCATCCGAGAAAAACGAGTACGCCCACTTGTTAGTTTCAGATTTCTCTTTGTCAGTGCCAACTGCCCCACCGGAAGTTCTGCAACAATTTTTGGGTGATCCAAGGGAGGGCGGTCATGCTCCCGGTAATGTTGCAAATGTTGGCAATCCAGTGGAGGGCGGCCATGCTCCACGCGATGTTGCAAATCGAAATGCAATCGCAGTCTTGTTGGAGTCATTTCTACCATGGGCTCATTATGGAGATAGAAACGGAGGCCGTGAGGAGGAAAATGAACAAAATGGACAGGGTCCAGGCAATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tcf25

Protein Analysis

626

Amino Acids

70.22

Weight (kDa)

5.82

Isoelectric Point (pI)

42.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tcf25 PF04910 227 - 552 2e-72 Transcriptional repressor TCF25
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 806
AccB1I GGYRCC 1 cut(s) 1336
AccI GTMKAC 1 cut(s) 1265
AccII CGCG 1 cut(s) 1749
AciI CCGC 4 cut(s) 478, 1112, 1686, 1734
AclWI GGATC 5 cut(s) 440, 453, 1061, 1425, 1667
AcoI YGGCCR 1 cut(s) 1735
AcsI RAATTY 5 cut(s) 116, 406, 647, 1183, 1447
AcuI CTGAAG 3 cut(s) 38, 1164, 1254
AfaI GTAC 5 cut(s) 455, 690, 821, 975, 1595
AfiI CCNNNNNNNGG 8 cut(s) 172, 919, 920, 1021, 1414, 1682, 1730, 1837
AflIII ACRYGT 1 cut(s) 55
AgsI TTSAA 7 cut(s) 31, 121, 710, 728, 1239, 1345, 1388
AjiI CACGTC 1 cut(s) 566
AjnI CCWGG 2 cut(s) 168, 1864
Alw21I GWGCWC 1 cut(s) 1249
Alw26I GTCTC 1 cut(s) 1508
Alw44I GTGCAC 1 cut(s) 1245
AlwI GGATC 5 cut(s) 440, 453, 1061, 1425, 1667
AoxI GGCC 5 cut(s) 231, 370, 1077, 1735, 1828
ApaLI GTGCAC 1 cut(s) 1245
ApeKI GCWGC 6 cut(s) 97, 383, 475, 569, 731, 1496
ApoI RAATTY 5 cut(s) 116, 406, 647, 1183, 1447
AspLEI GCGC 1 cut(s) 427
AspS9I GGNCC 3 cut(s) 371, 1077, 1862
AsuC2I CCSGG 2 cut(s) 374, 1698
AsuHPI GGTGA 6 cut(s) 514, 848, 901, 1295, 1412, 1682
AvaII GGWCC 1 cut(s) 1862
BaeGI GKGCMC 2 cut(s) 1249, 1363
BamHI GGATCC 1 cut(s) 445
BanI GGYRCC 1 cut(s) 1336
BanII GRGCYC 1 cut(s) 1807
BbsI GAAGAC 1 cut(s) 568
Bbv12I GWGCWC 1 cut(s) 1249
BbvCI CCTCAGC 1 cut(s) 1487
BbvI GCAGC 6 cut(s) 109, 370, 462, 556, 718, 1483
BccI CCATC 5 cut(s) 362, 623, 659, 1023, 1417
BceAI ACGGC 4 cut(s) 175, 403, 1194, 1815
BcgI CGANNNNNNTGC 2 cut(s) 602, 636
BciT130I CCWGG 2 cut(s) 170, 1866
BciVI GTATCC 1 cut(s) 662
BcnI CCSGG 2 cut(s) 374, 1698
BcoDI GTCTC 1 cut(s) 1508
BfaI CTAG 4 cut(s) 543, 582, 1055, 1130
BfuI GTATCC 1 cut(s) 662
BglII AGATCT 1 cut(s) 1471
BisI GCNGC 7 cut(s) 98, 384, 476, 570, 732, 1497, 1735
BlpI GCTNAGC 1 cut(s) 414
BlsI GCNGC 7 cut(s) 99, 385, 477, 571, 733, 1498, 1736
BmcAI AGTACT 2 cut(s) 455, 821
Bme1390I CCNGG 4 cut(s) 170, 374, 1698, 1866
Bme18I GGWCC 1 cut(s) 1862
BmgBI CACGTC 1 cut(s) 566
BmgT120I GGNCC 3 cut(s) 371, 1077, 1862
BmiI GGNNCC 4 cut(s) 447, 1078, 1338, 1863
BmrFI CCNGG 4 cut(s) 170, 374, 1698, 1866
BmsI GCATC 3 cut(s) 614, 628, 1483
BpiI GAAGAC 1 cut(s) 568
BplI GAGNNNNNCTC 2 cut(s) 344, 376
Bpu10I CCTNAGC 2 cut(s) 216, 1487
Bpu1102I GCTNAGC 1 cut(s) 414
BpuEI CTTGAG 2 cut(s) 371, 1241
BpuMI CCSGG 2 cut(s) 374, 1698
Bsa29I ATCGAT 1 cut(s) 1018
BsaAI YACGTR 1 cut(s) 58
BsaJI CCNNGG 7 cut(s) 169, 336, 642, 810, 1413, 1676, 1799
BsaWI WCCGGW 3 cut(s) 5, 1249, 1645
Bsc4I CCNNNNNNNGG 8 cut(s) 172, 919, 920, 1021, 1414, 1682, 1730, 1837
Bse118I RCCGGY 1 cut(s) 233
Bse1I ACTGG 2 cut(s) 387, 1724
Bse3DI GCAATG 1 cut(s) 1876
BseBI CCWGG 2 cut(s) 170, 1866
BseCI ATCGAT 1 cut(s) 1018
BseDI CCNNGG 7 cut(s) 169, 336, 642, 810, 1413, 1676, 1799
BseGI GGATG 8 cut(s) 181, 346, 373, 634, 698, 1409, 1498, 1577
BseLI CCNNNNNNNGG 8 cut(s) 172, 919, 920, 1021, 1414, 1682, 1730, 1837
BseMI GCAATG 1 cut(s) 1876
BseMII CTCAG 4 cut(s) 207, 728, 1323, 1478
BseNI ACTGG 2 cut(s) 387, 1724
BseRI GAGGAG 3 cut(s) 125, 1130, 1850
BseSI GKGCMC 2 cut(s) 1249, 1363
BseXI GCAGC 6 cut(s) 109, 370, 462, 556, 718, 1483
BseYI CCCAGC 1 cut(s) 624
BsgI GTGCAG 1 cut(s) 1482
Bsh1236I CGCG 1 cut(s) 1749
Bsh1285I CGRYCG 2 cut(s) 10, 1022
BshFI GGCC 5 cut(s) 233, 372, 1079, 1737, 1830
BshNI GGYRCC 1 cut(s) 1336
BshVI ATCGAT 1 cut(s) 1018
BsiEI CGRYCG 2 cut(s) 10, 1022
BsiHKAI GWGCWC 1 cut(s) 1249
BsiSI CCGG 6 cut(s) 6, 234, 374, 1250, 1646, 1698
BslFI GGGAC 1 cut(s) 164
BslI CCNNNNNNNGG 8 cut(s) 172, 919, 920, 1021, 1414, 1682, 1730, 1837
BsmAI GTCTC 1 cut(s) 1508
BsmFI GGGAC 1 cut(s) 164
BsmI GAATGC 2 cut(s) 18, 1571
BsnI GGCC 5 cut(s) 233, 372, 1079, 1737, 1830
Bsp1286I GDGCHC 3 cut(s) 1249, 1363, 1807
Bsp1407I TGTACA 1 cut(s) 688
Bsp1720I GCTNAGC 1 cut(s) 414
Bsp19I CCATGG 3 cut(s) 642, 810, 1799
BspACI CCGC 4 cut(s) 478, 1112, 1686, 1734
BspANI GGCC 5 cut(s) 233, 372, 1079, 1737, 1830
BspCNI CTCAG 4 cut(s) 208, 727, 1322, 1479
BspDI ATCGAT 1 cut(s) 1018
BspFNI CGCG 1 cut(s) 1749
BspLI GGNNCC 4 cut(s) 447, 1078, 1338, 1863
BspPI GGATC 5 cut(s) 440, 453, 1061, 1425, 1667
BspT107I GGYRCC 1 cut(s) 1336
BsrDI GCAATG 1 cut(s) 1876
BsrFI RCCGGY 1 cut(s) 233
BsrGI TGTACA 1 cut(s) 688
BsrI ACTGG 2 cut(s) 387, 1724
BssAI RCCGGY 1 cut(s) 233
BssECI CCNNGG 7 cut(s) 169, 336, 642, 810, 1413, 1676, 1799
BssT1I CCWWGG 6 cut(s) 336, 642, 810, 1413, 1676, 1799
Bst2UI CCWGG 2 cut(s) 170, 1866
Bst4CI ACNGT 4 cut(s) 190, 782, 973, 1137
BstAPI GCANNNNNTGC 1 cut(s) 1483
BstAUI TGTACA 1 cut(s) 688
BstBAI YACGTR 1 cut(s) 58
BstC8I GCNNGC 3 cut(s) 95, 1290, 1555
BstDEI CTNAG 9 cut(s) 216, 414, 495, 714, 935, 1260, 1309, 1487, 1520
BstDSI CCRYGG 3 cut(s) 642, 810, 1799
BstF5I GGATG 8 cut(s) 181, 346, 373, 634, 698, 1409, 1498, 1577
BstFNI CGCG 1 cut(s) 1749
BstHHI GCGC 1 cut(s) 427
BstMAI GTCTC 1 cut(s) 1508
BstMCI CGRYCG 2 cut(s) 10, 1022
BstMWI GCNNNNNNNGC 7 cut(s) 239, 424, 431, 719, 728, 737, 1483
BstNI CCWGG 2 cut(s) 170, 1866
BstNSI RCATGY 1 cut(s) 1557
BstSCI CCNGG 4 cut(s) 168, 372, 1696, 1864
BstSLI GKGCMC 2 cut(s) 1249, 1363
BstUI CGCG 1 cut(s) 1749
BstV1I GCAGC 6 cut(s) 109, 370, 462, 556, 718, 1483
BstV2I GAAGAC 1 cut(s) 568
BstX2I RGATCY 2 cut(s) 445, 1471
BstYI RGATCY 2 cut(s) 445, 1471
Bsu15I ATCGAT 1 cut(s) 1018
BsuI GTATCC 1 cut(s) 662
BsuRI GGCC 5 cut(s) 233, 372, 1079, 1737, 1830
BsuTUI ATCGAT 1 cut(s) 1018
BtgI CCRYGG 3 cut(s) 642, 810, 1799
BtgZI GCGATG 2 cut(s) 605, 1764
BtrI CACGTC 1 cut(s) 566
BtsCI GGATG 8 cut(s) 181, 346, 373, 634, 698, 1409, 1498, 1577
BtsI GCAGTG 1 cut(s) 600
BtsIMutI CAGTG 5 cut(s) 394, 600, 969, 1635, 1731
Cac8I GCNNGC 3 cut(s) 95, 1290, 1555
CfoI GCGC 1 cut(s) 427
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 3 cut(s) 371, 1077, 1862
ClaI ATCGAT 1 cut(s) 1018
CseI GACGC 1 cut(s) 231
Csp6I GTAC 5 cut(s) 454, 689, 820, 974, 1594
CviQI GTAC 5 cut(s) 454, 689, 820, 974, 1594
DdeI CTNAG 9 cut(s) 216, 414, 495, 714, 935, 1260, 1309, 1487, 1520
EaeI YGGCCR 1 cut(s) 1735
EciI GGCGGA 1 cut(s) 1127
Eco130I CCWWGG 6 cut(s) 336, 642, 810, 1413, 1676, 1799
Eco24I GRGCYC 1 cut(s) 1807
Eco47I GGWCC 1 cut(s) 1862
Eco57I CTGAAG 3 cut(s) 38, 1164, 1254
EcoRII CCWGG 2 cut(s) 168, 1864
EcoT14I CCWWGG 6 cut(s) 336, 642, 810, 1413, 1676, 1799
EcoT22I ATGCAT 2 cut(s) 1126, 1876
EcoT38I GRGCYC 1 cut(s) 1807
ErhI CCWWGG 6 cut(s) 336, 642, 810, 1413, 1676, 1799
FaqI GGGAC 1 cut(s) 164
FblI GTMKAC 1 cut(s) 1265
Fnu4HI GCNGC 7 cut(s) 98, 384, 476, 570, 732, 1497, 1735
FokI GGATG 8 cut(s) 188, 353, 380, 641, 685, 1396, 1505, 1564
FriOI GRGCYC 1 cut(s) 1807
Fsp4HI GCNGC 7 cut(s) 98, 384, 476, 570, 732, 1497, 1735
FspBI CTAG 4 cut(s) 543, 582, 1055, 1130
GlaI GCGC 1 cut(s) 426
GluI GCNGC 7 cut(s) 98, 384, 476, 570, 732, 1497, 1735
GsaI CCCAGC 1 cut(s) 628
HaeIII GGCC 5 cut(s) 233, 372, 1079, 1737, 1830
HapII CCGG 6 cut(s) 6, 234, 374, 1250, 1646, 1698
HgaI GACGC 1 cut(s) 231
HhaI GCGC 1 cut(s) 427
Hin6I GCGC 1 cut(s) 425
HinP1I GCGC 1 cut(s) 425
HincII GTYRAC 2 cut(s) 246, 466
HindII GTYRAC 2 cut(s) 246, 466
HpaII CCGG 6 cut(s) 6, 234, 374, 1250, 1646, 1698
HphI GGTGA 6 cut(s) 514, 848, 901, 1295, 1412, 1682
Hpy166II GTNNAC 7 cut(s) 246, 466, 839, 909, 969, 1247, 1266
Hpy188III TCNNGA 8 cut(s) 215, 350, 543, 865, 1220, 1441, 1469, 1513
Hpy8I GTNNAC 7 cut(s) 246, 466, 839, 909, 969, 1247, 1266
Hpy99I CGWCG 4 cut(s) 146, 149, 158, 161
HpyAV CCTTC 5 cut(s) 25, 71, 223, 716, 1339
HpyCH4III ACNGT 4 cut(s) 190, 782, 973, 1137
HpyCH4IV ACGT 2 cut(s) 57, 565
HpyF10VI GCNNNNNNNGC 7 cut(s) 239, 424, 431, 719, 728, 737, 1483
HpyF3I CTNAG 9 cut(s) 216, 414, 495, 714, 935, 1260, 1309, 1487, 1520
HpySE526I ACGT 2 cut(s) 57, 565
HspAI GCGC 1 cut(s) 425
LmnI GCTCC 2 cut(s) 1699, 1747
Lsp1109I GCAGC 6 cut(s) 109, 370, 462, 556, 718, 1483
LweI GCATC 3 cut(s) 614, 628, 1483
MaeI CTAG 4 cut(s) 543, 582, 1055, 1130
MaeII ACGT 2 cut(s) 57, 565
MaeIII GTNAC 3 cut(s) 435, 598, 1044
MfeI CAATTG 1 cut(s) 925
MflI RGATCY 2 cut(s) 445, 1471
MhlI GDGCHC 3 cut(s) 1249, 1363, 1807
MluCI AATT 9 cut(s) 116, 406, 647, 759, 925, 1183, 1322, 1447, 1661
MlyI GAGTC 2 cut(s) 362, 1796
MmeI TCCRAC 1 cut(s) 1764
Mph1103I ATGCAT 2 cut(s) 1126, 1876
MseI TTAA 2 cut(s) 458, 1371
MspA1I CMGCKG 1 cut(s) 1274
MspI CCGG 6 cut(s) 6, 234, 374, 1250, 1646, 1698
MspR9I CCNGG 4 cut(s) 170, 374, 1698, 1866
MunI CAATTG 1 cut(s) 925
Mva1269I GAATGC 2 cut(s) 18, 1571
MvaI CCWGG 2 cut(s) 170, 1866
MvnI CGCG 1 cut(s) 1749
MwoI GCNNNNNNNGC 7 cut(s) 239, 424, 431, 719, 728, 737, 1483
NciI CCSGG 2 cut(s) 374, 1698
NcoI CCATGG 3 cut(s) 642, 810, 1799
NlaIV GGNNCC 4 cut(s) 447, 1078, 1338, 1863
NmuCI GTSAC 1 cut(s) 598
NsiI ATGCAT 2 cut(s) 1126, 1876
NspI RCATGY 1 cut(s) 1557
PaeI GCATGC 1 cut(s) 1557
PcsI WCGNNNNNNNCGW 3 cut(s) 150, 153, 159
PctI GAATGC 2 cut(s) 18, 1571
PflFI GACNNNGTC 1 cut(s) 1860
PkrI GCNGC 7 cut(s) 99, 385, 477, 571, 733, 1498, 1736
Ple19I CGATCG 1 cut(s) 1022
PleI GAGTC 2 cut(s) 361, 1795
PpsI GAGTC 2 cut(s) 361, 1795
Ppu21I YACGTR 1 cut(s) 58
PsiI TTATAA 1 cut(s) 806
Psp6I CCWGG 2 cut(s) 168, 1864
PspFI CCCAGC 1 cut(s) 624
PspGI CCWGG 2 cut(s) 168, 1864
PspN4I GGNNCC 4 cut(s) 447, 1078, 1338, 1863
PspPI GGNCC 3 cut(s) 371, 1077, 1862
PsuI RGATCY 2 cut(s) 445, 1471
PsyI GACNNNGTC 1 cut(s) 1860
PvuI CGATCG 1 cut(s) 1022
PvuII CAGCTG 1 cut(s) 1274
RsaI GTAC 5 cut(s) 455, 690, 821, 975, 1595
RsaNI GTAC 5 cut(s) 454, 689, 820, 974, 1594
SaqAI TTAA 2 cut(s) 458, 1371
SatI GCNGC 7 cut(s) 98, 384, 476, 570, 732, 1497, 1735
Sau96I GGNCC 3 cut(s) 371, 1077, 1862
ScaI AGTACT 2 cut(s) 455, 821
SchI GAGTC 2 cut(s) 362, 1796
ScrFI CCNGG 4 cut(s) 170, 374, 1698, 1866
SduI GDGCHC 3 cut(s) 1249, 1363, 1807
SfaNI GCATC 3 cut(s) 614, 628, 1483
SinI GGWCC 1 cut(s) 1862
SmlI CTYRAG 2 cut(s) 350, 1220
SmoI CTYRAG 2 cut(s) 350, 1220
SphI GCATGC 1 cut(s) 1557
Sse9I AATT 9 cut(s) 116, 406, 647, 759, 925, 1183, 1322, 1447, 1661
SsiI CCGC 4 cut(s) 478, 1112, 1686, 1734
SspMI CTAG 4 cut(s) 543, 582, 1055, 1130
StyD4I CCNGG 4 cut(s) 168, 372, 1696, 1864
StyI CCWWGG 6 cut(s) 336, 642, 810, 1413, 1676, 1799
TaaI ACNGT 4 cut(s) 190, 782, 973, 1137
TaiI ACGT 2 cut(s) 60, 568
TaqI TCGA 7 cut(s) 10, 682, 866, 1018, 1440, 1512, 1762
TasI AATT 9 cut(s) 116, 406, 647, 759, 925, 1183, 1322, 1447, 1661
TatI WGTACW 4 cut(s) 453, 688, 819, 973
TauI GCSGC 1 cut(s) 1737
Tru1I TTAA 2 cut(s) 458, 1371
Tru9I TTAA 2 cut(s) 458, 1371
TscAI CASTG 5 cut(s) 394, 607, 976, 1635, 1731
TseFI GTSAC 1 cut(s) 598
TseI GCWGC 6 cut(s) 97, 383, 475, 569, 731, 1496
Tsp45I GTSAC 1 cut(s) 598
TspDTI ATGAA 6 cut(s) 192, 210, 963, 1179, 1298, 1860
TspGWI ACGGA 3 cut(s) 66, 120, 1839
TspRI CASTG 5 cut(s) 394, 607, 976, 1635, 1731
Tth111I GACNNNGTC 1 cut(s) 1860
VneI GTGCAC 1 cut(s) 1245
VpaK11BI GGWCC 1 cut(s) 1862
XapI RAATTY 5 cut(s) 116, 406, 647, 1183, 1447
XbaI TCTAGA 1 cut(s) 542
XceI RCATGY 1 cut(s) 1557
XmiI GTMKAC 1 cut(s) 1265
XspI CTAG 4 cut(s) 543, 582, 1055, 1130
ZrmI AGTACT 2 cut(s) 455, 821
Zsp2I ATGCAT 2 cut(s) 1126, 1876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.