Rh1AG302500

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0007094
Physical Location & Seq
Forward (+)
53456871 .. 53461061
4191 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG302500.1

Sequence Viewer

Length: 1401 bp
ATGCGAAGAATCTTTGGGGCTAAGGTGGTCAAGTCGTTTGAGAAGAATCAACAGAGTGGTAGCTCTAGAATGGCGCGTGGTGGAAGACGCGGCGCGGTTCATAGGAAGTCTTTCCTTGTCACTCCGTTGGAGCATTGGCCTCGCTGGGACGGTGCTATGAACATGGAACTCCTTCACTCCCCCAATGGATACTATGATTACCGATATACCTATTCATCAGCCTACCATCAAGCTCAGAGTGCATTTGAATCTGCCAAAGCTATACATGATCTCAATGCCATTGCAAGTGTTTTATTATACTATCCTTACCATTTGGATTCGCTACTAACCATGGCAGAGTACTTTAAGTTTGTGGGTGAGCATCAAATGTCAGCAGATGCTATCTCGAAGTGCTTATATGCTTTGGAATGTGCATGGTCTCCAATGTTCACCCCCTTTAAGGGTGATTGCCAAGTGAAATACAGCCATGAAGCAAACAAACCTCTGTTCGTTGCACTTTTTACTCACATGAAAAGTATGGACAGACGTGGCTGTCATCGATCTGCTTTAGAAGTTTGCAAGTTTTTACTGTCACTAGATACTGACGATCCAATGGGGGCCATGTTCTGTATTGACTATTTTGCGGTGAGGGCAGAGGAGTATGCATGGCTAGAACGTTTCTCTGAAGAATATAAAAGTGATAACACCCTATGGTTGTTTCCAAATTTTTCATATTCCCTTGCCGTATGCCGATTGTATCTTGAGAAAGCAGAATCTTCCAAAGATCCAGCTATGGAAACCACCAAGGCTACTTCGACTGATCTTATGAAGCAGGCATTGATGCTTCATCCCACAGTCCTCAAGAAACTAGTGGCAAAAGTACCTTTGAAGGAACGGGTGTGGACGGATATACTCAAAAATGCATTCTTCCAAGCAGATGAAGTAGGAATCCCATCCTTGGACCACCTGATTAACATATACGTTGAGAGTAATCATATTATATGGAGGCTTCCAGAATTGCAGAAATTGCTGCGGGAAGCAGCACAGCTGGTGATCGAGACTCTAAGACAGAATAGTAGCGAAAAGAATGATTGGGCATGTGTGAGAAAAGAAGCATTCTCATCTGACAAGAATGAGTATTCCCACTTGTTGGTTTCAGACTTCTCTTATACATTGCCTGCTGCCCCACCTGAAAATTTGCAACATTTTATGGCCAATGCAAGGATGGAGGATGTTGTGCATAATGAGCAGCATCTTGTCAATCTAAATGAGGGTGGCCATGCTCCCCGTGATGTTGCAAATCGAAATCCATTAGCGGTCTTGTTTGAGTCGGTGTTACCATGGGTTCATTATGGGGAAGGAGCGTTTGTTGAGGAGGAAGACCAACAAAATGGCCATGGTCCGGGCAATCAAGAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tcf25

Protein Analysis

466

Amino Acids

53.49

Weight (kDa)

6.09

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tcf25 PF04910 67 - 391 4.9e-69 Transcriptional repressor TCF25
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 531
AccB7I CCANNNNNTGG 1 cut(s) 1129
AccII CGCG 3 cut(s) 76, 90, 95
AciI CCGC 5 cut(s) 90, 95, 623, 1012, 1295
AclI AACGTT 1 cut(s) 655
AclWI GGATC 2 cut(s) 581, 758
AcoI YGGCCR 3 cut(s) 1191, 1255, 1372
AcsI RAATTY 2 cut(s) 703, 1174
AcuI CTGAAG 1 cut(s) 684
AfaI GTAC 2 cut(s) 341, 861
AfiI CCNNNNNNNGG 6 cut(s) 439, 440, 937, 1129, 1200, 1381
AgsI TTSAA 2 cut(s) 248, 868
AhlI ACTAGT 1 cut(s) 847
AjiI CACGTC 1 cut(s) 527
AluBI AGCT 5 cut(s) 63, 233, 260, 770, 1027
AluI AGCT 5 cut(s) 63, 233, 260, 770, 1027
Alw26I GTCTC 2 cut(s) 423, 1031
AlwI GGATC 2 cut(s) 581, 758
AoxI GGCC 5 cut(s) 137, 597, 1191, 1255, 1372
ApeKI GCWGC 4 cut(s) 1009, 1019, 1160, 1228
ApoI RAATTY 2 cut(s) 703, 1174
Asp700I GAANNNNTTC 2 cut(s) 110, 171
AspLEI GCGC 2 cut(s) 76, 95
AspS9I GGNCC 3 cut(s) 597, 940, 1379
AsuC2I CCSGG 1 cut(s) 1383
AsuHPI GGTGA 5 cut(s) 368, 421, 455, 637, 1042
AvaII GGWCC 2 cut(s) 940, 1379
BalI TGGCCA 3 cut(s) 1193, 1257, 1374
BbsI GAAGAC 2 cut(s) 91, 1365
BbvI GCAGC 4 cut(s) 996, 1031, 1147, 1240
BccI CCATC 3 cut(s) 234, 940, 1198
BceAI ACGGC 1 cut(s) 707
BcgI CGANNNNNNTGC 2 cut(s) 122, 156
BciVI GTATCC 1 cut(s) 182
BcnI CCSGG 1 cut(s) 1383
BcoDI GTCTC 2 cut(s) 423, 1031
BcuI ACTAGT 1 cut(s) 847
BfaI CTAG 4 cut(s) 66, 575, 650, 848
BfuI GTATCC 1 cut(s) 182
BisI GCNGC 5 cut(s) 91, 1010, 1020, 1161, 1229
BlsI GCNGC 5 cut(s) 92, 1011, 1021, 1162, 1230
BmcAI AGTACT 1 cut(s) 341
Bme1390I CCNGG 1 cut(s) 1383
Bme18I GGWCC 2 cut(s) 940, 1379
BmgBI CACGTC 1 cut(s) 527
BmgT120I GGNCC 3 cut(s) 597, 940, 1379
BmiI GGNNCC 1 cut(s) 598
BmrFI CCNGG 1 cut(s) 1383
BmsI GCATC 4 cut(s) 367, 370, 810, 1240
BpiI GAAGAC 2 cut(s) 91, 1365
Bpu10I CCTNAGC 1 cut(s) 21
BpuEI CTTGAG 2 cut(s) 761, 824
BpuMI CCSGG 1 cut(s) 1383
Bsa29I ATCGAT 1 cut(s) 538
BsaI GGTCTC 1 cut(s) 423
BsaJI CCNNGG 5 cut(s) 330, 783, 936, 1319, 1375
BsaXI ACNNNNNCTCC 2 cut(s) 1332, 1362
Bsc4I CCNNNNNNNGG 6 cut(s) 439, 440, 937, 1129, 1200, 1381
Bse3DI GCAATG 2 cut(s) 279, 1151
BseCI ATCGAT 1 cut(s) 538
BseDI CCNNGG 5 cut(s) 330, 783, 936, 1319, 1375
BseGI GGATG 4 cut(s) 826, 932, 1209, 1216
BseLI CCNNNNNNNGG 6 cut(s) 439, 440, 937, 1129, 1200, 1381
BseMI GCAATG 2 cut(s) 279, 1151
BseMII CTCAG 1 cut(s) 248
BseRI GAGGAG 2 cut(s) 650, 1367
BseXI GCAGC 4 cut(s) 996, 1031, 1147, 1240
BseYI CCCAGC 1 cut(s) 144
Bsh1236I CGCG 3 cut(s) 76, 90, 95
BshFI GGCC 5 cut(s) 139, 599, 1193, 1257, 1374
BshVI ATCGAT 1 cut(s) 538
BsiSI CCGG 1 cut(s) 1382
BslFI GGGAC 1 cut(s) 161
BslI CCNNNNNNNGG 6 cut(s) 439, 440, 937, 1129, 1200, 1381
BsmAI GTCTC 2 cut(s) 423, 1031
BsmFI GGGAC 1 cut(s) 161
BsmI GAATGC 2 cut(s) 902, 1094
BsnI GGCC 5 cut(s) 139, 599, 1193, 1257, 1374
Bso31I GGTCTC 1 cut(s) 423
Bsp143I GATC 6 cut(s) 268, 539, 586, 763, 799, 1032
Bsp19I CCATGG 3 cut(s) 330, 1319, 1375
BspACI CCGC 5 cut(s) 90, 95, 623, 1012, 1295
BspANI GGCC 5 cut(s) 139, 599, 1193, 1257, 1374
BspCNI CTCAG 1 cut(s) 247
BspDI ATCGAT 1 cut(s) 538
BspFNI CGCG 3 cut(s) 76, 90, 95
BspLI GGNNCC 1 cut(s) 598
BspPI GGATC 2 cut(s) 581, 758
BspTNI GGTCTC 1 cut(s) 423
BsrDI GCAATG 2 cut(s) 279, 1151
BssECI CCNNGG 5 cut(s) 330, 783, 936, 1319, 1375
BssMI GATC 6 cut(s) 268, 539, 586, 763, 799, 1032
BssT1I CCWWGG 5 cut(s) 330, 783, 936, 1319, 1375
Bst4CI ACNGT 3 cut(s) 152, 570, 835
BstAPI GCANNNNNTGC 1 cut(s) 1006
BstC8I GCNNGC 2 cut(s) 813, 1158
BstDEI CTNAG 3 cut(s) 21, 234, 1043
BstDSI CCRYGG 3 cut(s) 330, 1319, 1375
BstF5I GGATG 4 cut(s) 826, 932, 1209, 1216
BstFNI CGCG 3 cut(s) 76, 90, 95
BstHHI GCGC 2 cut(s) 76, 95
BstKTI GATC 6 cut(s) 271, 542, 589, 766, 802, 1035
BstMAI GTCTC 2 cut(s) 423, 1031
BstMBI GATC 6 cut(s) 268, 539, 586, 763, 799, 1032
BstMWI GCNNNNNNNGC 4 cut(s) 239, 629, 1006, 1225
BstNSI RCATGY 1 cut(s) 1080
BstSCI CCNGG 1 cut(s) 1381
BstUI CGCG 3 cut(s) 76, 90, 95
BstV1I GCAGC 4 cut(s) 996, 1031, 1147, 1240
BstV2I GAAGAC 2 cut(s) 91, 1365
BstX2I RGATCY 1 cut(s) 763
BstXI CCANNNNNNTGG 1 cut(s) 1370
BstYI RGATCY 1 cut(s) 763
Bsu15I ATCGAT 1 cut(s) 538
BsuI GTATCC 1 cut(s) 182
BsuRI GGCC 5 cut(s) 139, 599, 1193, 1257, 1374
BsuTUI ATCGAT 1 cut(s) 538
BtgI CCRYGG 3 cut(s) 330, 1319, 1375
BtrI CACGTC 1 cut(s) 527
BtsCI GGATG 4 cut(s) 826, 932, 1209, 1216
Cac8I GCNNGC 2 cut(s) 813, 1158
CfoI GCGC 2 cut(s) 76, 95
Cfr13I GGNCC 3 cut(s) 597, 940, 1379
ClaI ATCGAT 1 cut(s) 538
CseI GACGC 1 cut(s) 96
Csp6I GTAC 2 cut(s) 340, 860
CviQI GTAC 2 cut(s) 340, 860
DdeI CTNAG 3 cut(s) 21, 234, 1043
DpnI GATC 6 cut(s) 270, 541, 588, 765, 801, 1034
DpnII GATC 6 cut(s) 268, 539, 586, 763, 799, 1032
DrdI GACNNNNNNGTC 1 cut(s) 531
DseDI GACNNNNNNGTC 1 cut(s) 531
EaeI YGGCCR 3 cut(s) 1191, 1255, 1372
Eco130I CCWWGG 5 cut(s) 330, 783, 936, 1319, 1375
Eco31I GGTCTC 1 cut(s) 423
Eco47I GGWCC 2 cut(s) 940, 1379
Eco57I CTGAAG 1 cut(s) 684
EcoT14I CCWWGG 5 cut(s) 330, 783, 936, 1319, 1375
EcoT22I ATGCAT 2 cut(s) 646, 904
ErhI CCWWGG 5 cut(s) 330, 783, 936, 1319, 1375
FaqI GGGAC 1 cut(s) 161
FauI CCCGC 1 cut(s) 1005
Fnu4HI GCNGC 5 cut(s) 91, 1010, 1020, 1161, 1229
FokI GGATG 4 cut(s) 813, 919, 1216, 1223
Fsp4HI GCNGC 5 cut(s) 91, 1010, 1020, 1161, 1229
FspBI CTAG 4 cut(s) 66, 575, 650, 848
GlaI GCGC 2 cut(s) 75, 94
GluI GCNGC 5 cut(s) 91, 1010, 1020, 1161, 1229
GsaI CCCAGC 1 cut(s) 148
HaeIII GGCC 5 cut(s) 139, 599, 1193, 1257, 1374
HapII CCGG 1 cut(s) 1382
HgaI GACGC 1 cut(s) 96
HhaI GCGC 2 cut(s) 76, 95
Hin6I GCGC 2 cut(s) 74, 93
HinP1I GCGC 2 cut(s) 74, 93
HinfI GANTC 8 cut(s) 9, 46, 248, 317, 752, 927, 1039, 1307
HpaII CCGG 1 cut(s) 1382
HphI GGTGA 5 cut(s) 368, 421, 455, 637, 1042
Hpy166II GTNNAC 2 cut(s) 429, 882
Hpy188I TCNGA 4 cut(s) 237, 664, 1105, 1138
Hpy188III TCNNGA 7 cut(s) 66, 385, 740, 841, 992, 1036, 1391
Hpy8I GTNNAC 2 cut(s) 429, 882
HpyAV CCTTC 3 cut(s) 182, 862, 1331
HpyCH4III ACNGT 3 cut(s) 152, 570, 835
HpyCH4IV ACGT 3 cut(s) 526, 655, 960
HpyF10VI GCNNNNNNNGC 4 cut(s) 239, 629, 1006, 1225
HpyF3I CTNAG 3 cut(s) 21, 234, 1043
HpySE526I ACGT 3 cut(s) 526, 655, 960
HspAI GCGC 2 cut(s) 74, 93
Kzo9I GATC 6 cut(s) 268, 539, 586, 763, 799, 1032
LmnI GCTCC 3 cut(s) 130, 1267, 1340
LpnPI CCDG 9 cut(s) 130, 780, 797, 959, 1005, 1013, 1170, 1182, 1395
Lsp1109I GCAGC 4 cut(s) 996, 1031, 1147, 1240
LweI GCATC 4 cut(s) 367, 370, 810, 1240
MaeI CTAG 4 cut(s) 66, 575, 650, 848
MaeII ACGT 3 cut(s) 526, 655, 960
MaeIII GTNAC 3 cut(s) 118, 570, 1314
MalI GATC 6 cut(s) 270, 541, 588, 765, 801, 1034
MboI GATC 6 cut(s) 268, 539, 586, 763, 799, 1032
MboII GAAGA 7 cut(s) 18, 55, 96, 677, 747, 898, 1370
MflI RGATCY 1 cut(s) 763
MlsI TGGCCA 3 cut(s) 1193, 1257, 1374
MluCI AATT 4 cut(s) 703, 995, 1004, 1174
MluNI TGGCCA 3 cut(s) 1193, 1257, 1374
MlyI GAGTC 2 cut(s) 1033, 1316
MmeI TCCRAC 1 cut(s) 108
Mox20I TGGCCA 3 cut(s) 1193, 1257, 1374
Mph1103I ATGCAT 2 cut(s) 646, 904
MroXI GAANNNNTTC 2 cut(s) 110, 171
MscI TGGCCA 3 cut(s) 1193, 1257, 1374
MseI TTAA 3 cut(s) 345, 438, 951
Msp20I TGGCCA 3 cut(s) 1193, 1257, 1374
MspA1I CMGCKG 1 cut(s) 1027
MspI CCGG 1 cut(s) 1382
MspR9I CCNGG 1 cut(s) 1383
Mva1269I GAATGC 2 cut(s) 902, 1094
MvnI CGCG 3 cut(s) 76, 90, 95
MwoI GCNNNNNNNGC 4 cut(s) 239, 629, 1006, 1225
NciI CCSGG 1 cut(s) 1383
NcoI CCATGG 3 cut(s) 330, 1319, 1375
NdeII GATC 6 cut(s) 268, 539, 586, 763, 799, 1032
NlaIV GGNNCC 1 cut(s) 598
NmuCI GTSAC 2 cut(s) 118, 570
NsiI ATGCAT 2 cut(s) 646, 904
NspI RCATGY 1 cut(s) 1080
PctI GAATGC 2 cut(s) 902, 1094
PdmI GAANNNNTTC 2 cut(s) 110, 171
PfeI GAWTC 6 cut(s) 9, 46, 248, 317, 752, 927
PflMI CCANNNNNTGG 1 cut(s) 1129
PkrI GCNGC 5 cut(s) 92, 1011, 1021, 1162, 1230
PleI GAGTC 2 cut(s) 1033, 1315
PpsI GAGTC 2 cut(s) 1033, 1315
Psp1406I AACGTT 1 cut(s) 655
PspFI CCCAGC 1 cut(s) 144
PspN4I GGNNCC 1 cut(s) 598
PspPI GGNCC 3 cut(s) 597, 940, 1379
PsuI RGATCY 1 cut(s) 763
PvuII CAGCTG 1 cut(s) 1027
RsaI GTAC 2 cut(s) 341, 861
RsaNI GTAC 2 cut(s) 340, 860
SaqAI TTAA 3 cut(s) 345, 438, 951
SatI GCNGC 5 cut(s) 91, 1010, 1020, 1161, 1229
Sau3AI GATC 6 cut(s) 268, 539, 586, 763, 799, 1032
Sau96I GGNCC 3 cut(s) 597, 940, 1379
ScaI AGTACT 1 cut(s) 341
SchI GAGTC 2 cut(s) 1033, 1316
ScrFI CCNGG 1 cut(s) 1383
SfaNI GCATC 4 cut(s) 367, 370, 810, 1240
SinI GGWCC 2 cut(s) 940, 1379
SmlI CTYRAG 2 cut(s) 740, 839
SmoI CTYRAG 2 cut(s) 740, 839
SpeI ACTAGT 1 cut(s) 847
Sse9I AATT 4 cut(s) 703, 995, 1004, 1174
SsiI CCGC 5 cut(s) 90, 95, 623, 1012, 1295
SspMI CTAG 4 cut(s) 66, 575, 650, 848
StyD4I CCNGG 1 cut(s) 1381
StyI CCWWGG 5 cut(s) 330, 783, 936, 1319, 1375
TaaI ACNGT 3 cut(s) 152, 570, 835
TaiI ACGT 3 cut(s) 529, 658, 963
TaqI TCGA 5 cut(s) 386, 538, 794, 1035, 1282
TasI AATT 4 cut(s) 703, 995, 1004, 1174
TatI WGTACW 1 cut(s) 339
TauI GCSGC 1 cut(s) 93
TfiI GAWTC 6 cut(s) 9, 46, 248, 317, 752, 927
Tru1I TTAA 3 cut(s) 345, 438, 951
Tru9I TTAA 3 cut(s) 345, 438, 951
TseFI GTSAC 2 cut(s) 118, 570
TseI GCWGC 4 cut(s) 1009, 1019, 1160, 1228
Tsp45I GTSAC 2 cut(s) 118, 570
TspGWI ACGGA 2 cut(s) 114, 899
Van91I CCANNNNNTGG 1 cut(s) 1129
VpaK11BI GGWCC 2 cut(s) 940, 1379
XapI RAATTY 2 cut(s) 703, 1174
XbaI TCTAGA 1 cut(s) 65
XceI RCATGY 1 cut(s) 1080
XcmI CCANNNNNNNNNTGG 1 cut(s) 1201
XmnI GAANNNNTTC 2 cut(s) 110, 171
XspI CTAG 4 cut(s) 66, 575, 650, 848
ZrmI AGTACT 1 cut(s) 341
Zsp2I ATGCAT 2 cut(s) 646, 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.