MD01G1122000.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003808
Physical Location & Seq
Reverse (-)
23530357 .. 23531554
1198 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1122000.v1.1.491

Sequence Viewer

Length: 984 bp
ATGGTGAAGGAAATAGCAGATGATTTGTTTGGAAAATTACCAAATGAAATCATTTGCAAAATTATTTCTTTTCTCCCTTCAGAATCTGCATTAGAAACCAGTTTAATTTCAAGTATGTGGAGAGGCCTTTGGAATACAACTTTAGTACAGCGGGTCACTACAGATGCTGCTGCTGCTGATGCAGTTTCTGAGTTTCTATCTCACTTTGACCAACATGATCCCTTAGGGCATCCCAGGAAGCTGCAACTGCACTTTGGGAAACGCAGTGATCTCCTATTGGCTACAGTTGCAGCCAACAATAAACTCCACCTGGAGTTTTCCACAGAGAATCAGGAAATTCCAAGGCATTTTGGTTGGAACTTGAAGCTGAATCACCAAAGGGTCCAAACCCTAATTTCCCACCAGCCTTCTCCGTCTACTTTCTTTGTCAAAATTCTTTACCTAAAATCAGTCAGCTCTTTTGGCAATGAAGCAATTTCTTCTATGATTCCGATTTTCGAATTTCTCGAAAACTTGAAGATCATTGAATGTGAAGGGTTACAATCTTTGCATATCGAGTCCAGTCCAAGGCTACTCAGCCTGGCCATCTTTGACTGCCAAGATCTGAAGTCTCTCCACCTCAAAACTTCAAAACTTCGATCTTTTCGATACCGTGGTTTTCTCCCTCAGATTTGGACGGAACATCATTTTAACTTAGCAGACGCCATGCTCGATTTCAGGCAAGGCCCCATCCACAGCGGATTCAAAAGTACCGACTTTGATCCTATGTTGTTAACCATTAAAAATGCTCAAGTTCTCACACTAAGCAAATGGACTTTTCAGACATTGATCTGGCCATCCCTTTTGTCACTACAAGCAAACTTCATATTCTACAAGCTGAAAGAGCTACGGTGGATTGATAATCGGGAAGAAAGATGCAACAGCGATGCTCTAATGTCCTTTATTAAATTATGTCCAGCTTTGGAGCAACTCTCTCTAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

328

Amino Acids

37.56

Weight (kDa)

6.9

Isoelectric Point (pI)

40.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 145 - 326 1.4e-07 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 416
AciI CCGC 2 cut(s) 151, 738
AclWI GGATC 2 cut(s) 212, 755
AcoI YGGCCR 2 cut(s) 582, 833
AcsI RAATTY 3 cut(s) 336, 432, 500
AcuI CTGAAG 2 cut(s) 63, 626
AcyI GRCGYC 1 cut(s) 702
AfaI GTAC 2 cut(s) 147, 751
AfiI CCNNNNNNNGG 1 cut(s) 567
AgsI TTSAA 6 cut(s) 111, 364, 517, 527, 630, 745
AjnI CCWGG 3 cut(s) 233, 309, 579
AluBI AGCT 6 cut(s) 241, 367, 456, 877, 886, 959
AluI AGCT 6 cut(s) 241, 367, 456, 877, 886, 959
Alw26I GTCTC 1 cut(s) 615
AlwI GGATC 2 cut(s) 212, 755
AlwNI CAGNNNCTG 3 cut(s) 86, 167, 188
AoxI GGCC 4 cut(s) 124, 582, 724, 833
ApeKI GCWGC 5 cut(s) 167, 170, 173, 241, 290
ApoI RAATTY 3 cut(s) 336, 432, 500
AspS9I GGNCC 2 cut(s) 382, 725
AsuHPI GGTGA 2 cut(s) 16, 365
AsuII TTCGAA 1 cut(s) 498
AvaII GGWCC 1 cut(s) 382
AxyI CCTNAGG 1 cut(s) 223
BaeI ACNNNNGTAYC 2 cut(s) 640, 673
BalI TGGCCA 2 cut(s) 584, 835
BbvI GCAGC 5 cut(s) 154, 157, 160, 228, 302
BccI CCATC 3 cut(s) 593, 737, 844
BciT130I CCWGG 3 cut(s) 235, 311, 581
BcoDI GTCTC 1 cut(s) 615
BfmI CTRYAG 2 cut(s) 159, 282
BglII AGATCT 1 cut(s) 601
BisI GCNGC 5 cut(s) 168, 171, 174, 242, 291
BlsI GCNGC 5 cut(s) 169, 172, 175, 243, 292
Bme1390I CCNGG 3 cut(s) 235, 311, 581
Bme18I GGWCC 1 cut(s) 382
BmgT120I GGNCC 2 cut(s) 382, 725
BmiI GGNNCC 2 cut(s) 383, 727
BmrFI CCNGG 3 cut(s) 235, 311, 581
BmsI GCATC 5 cut(s) 154, 169, 238, 905, 916
BplI GAGNNNNNCTC 1 cut(s) 956
BpmI CTGGAG 1 cut(s) 332
Bpu14I TTCGAA 1 cut(s) 498
BpuEI CTTGAG 1 cut(s) 774
BsaHI GRCGYC 1 cut(s) 702
BsaJI CCNNGG 4 cut(s) 233, 341, 566, 652
Bsc4I CCNNNNNNNGG 1 cut(s) 567
Bse1I ACTGG 2 cut(s) 99, 561
Bse21I CCTNAGG 1 cut(s) 223
Bse3DI GCAATG 1 cut(s) 472
BseBI CCWGG 3 cut(s) 235, 311, 581
BseDI CCNNGG 4 cut(s) 233, 341, 566, 652
BseGI GGATG 3 cut(s) 229, 729, 836
BseLI CCNNNNNNNGG 1 cut(s) 567
BseMI GCAATG 1 cut(s) 472
BseMII CTCAG 3 cut(s) 180, 589, 680
BseNI ACTGG 2 cut(s) 99, 561
BseXI GCAGC 5 cut(s) 154, 157, 160, 228, 302
BsgI GTGCAG 1 cut(s) 233
BshFI GGCC 4 cut(s) 126, 584, 726, 835
BslI CCNNNNNNNGG 1 cut(s) 567
BsmAI GTCTC 1 cut(s) 615
BsnI GGCC 4 cut(s) 126, 584, 726, 835
Bsp119I TTCGAA 1 cut(s) 498
Bsp143I GATC 7 cut(s) 217, 268, 519, 601, 638, 760, 828
BspACI CCGC 2 cut(s) 151, 738
BspANI GGCC 4 cut(s) 126, 584, 726, 835
BspCNI CTCAG 3 cut(s) 181, 588, 679
BspLI GGNNCC 2 cut(s) 383, 727
BspPI GGATC 2 cut(s) 212, 755
BspT104I TTCGAA 1 cut(s) 498
BsrDI GCAATG 1 cut(s) 472
BsrI ACTGG 2 cut(s) 99, 561
BssECI CCNNGG 4 cut(s) 233, 341, 566, 652
BssMI GATC 7 cut(s) 217, 268, 519, 601, 638, 760, 828
BssNI GRCGYC 1 cut(s) 702
BssT1I CCWWGG 2 cut(s) 341, 566
Bst2UI CCWGG 3 cut(s) 235, 311, 581
Bst4CI ACNGT 3 cut(s) 286, 653, 891
BstACI GRCGYC 1 cut(s) 702
BstBI TTCGAA 1 cut(s) 498
BstDEI CTNAG 6 cut(s) 189, 223, 575, 666, 694, 803
BstDSI CCRYGG 1 cut(s) 652
BstF5I GGATG 3 cut(s) 229, 729, 836
BstKTI GATC 7 cut(s) 220, 271, 522, 604, 641, 763, 831
BstMAI GTCTC 1 cut(s) 615
BstMBI GATC 7 cut(s) 217, 268, 519, 601, 638, 760, 828
BstMWI GCNNNNNNNGC 6 cut(s) 173, 179, 247, 287, 462, 883
BstNI CCWGG 3 cut(s) 235, 311, 581
BstSCI CCNGG 3 cut(s) 233, 309, 579
BstSFI CTRYAG 2 cut(s) 159, 282
BstV1I GCAGC 5 cut(s) 154, 157, 160, 228, 302
BstX2I RGATCY 1 cut(s) 601
BstYI RGATCY 1 cut(s) 601
Bsu36I CCTNAGG 1 cut(s) 223
BsuRI GGCC 4 cut(s) 126, 584, 726, 835
BtgI CCRYGG 1 cut(s) 652
BtgZI GCGATG 1 cut(s) 939
BtsCI GGATG 3 cut(s) 229, 729, 836
BtsI GCAGTG 1 cut(s) 271
BtsIMutI CAGTG 1 cut(s) 271
CaiI CAGNNNCTG 3 cut(s) 86, 167, 188
Cfr13I GGNCC 2 cut(s) 382, 725
CseI GACGC 1 cut(s) 710
Csp6I GTAC 2 cut(s) 146, 750
CviAII CATG 2 cut(s) 215, 706
CviQI GTAC 2 cut(s) 146, 750
DdeI CTNAG 6 cut(s) 189, 223, 575, 666, 694, 803
DpnI GATC 7 cut(s) 219, 270, 521, 603, 640, 762, 830
DpnII GATC 7 cut(s) 217, 268, 519, 601, 638, 760, 828
EaeI YGGCCR 2 cut(s) 582, 833
Eco130I CCWWGG 2 cut(s) 341, 566
Eco147I AGGCCT 1 cut(s) 126
Eco47I GGWCC 1 cut(s) 382
Eco57I CTGAAG 2 cut(s) 63, 626
Eco81I CCTNAGG 1 cut(s) 223
EcoO109I RGGNCCY 1 cut(s) 725
EcoRII CCWGG 3 cut(s) 233, 309, 579
EcoT14I CCWWGG 2 cut(s) 341, 566
ErhI CCWWGG 2 cut(s) 341, 566
FaeI CATG 2 cut(s) 218, 709
FaiI YATR 8 cut(s) 116, 216, 485, 552, 707, 767, 866, 952
FatI CATG 2 cut(s) 214, 705
FauI CCCGC 1 cut(s) 144
FblI GTMKAC 1 cut(s) 416
Fnu4HI GCNGC 5 cut(s) 168, 171, 174, 242, 291
FokI GGATG 3 cut(s) 216, 716, 823
Fsp4HI GCNGC 5 cut(s) 168, 171, 174, 242, 291
GluI GCNGC 5 cut(s) 168, 171, 174, 242, 291
GsuI CTGGAG 1 cut(s) 332
HaeIII GGCC 4 cut(s) 126, 584, 726, 835
HgaI GACGC 1 cut(s) 710
Hin1I GRCGYC 1 cut(s) 702
Hin1II CATG 2 cut(s) 218, 709
HincII GTYRAC 1 cut(s) 774
HindII GTYRAC 1 cut(s) 774
HinfI GANTC 6 cut(s) 83, 328, 370, 487, 557, 741
HpaI GTTAAC 1 cut(s) 774
HphI GGTGA 2 cut(s) 16, 365
Hpy166II GTNNAC 2 cut(s) 417, 774
Hpy188I TCNGA 6 cut(s) 82, 190, 492, 606, 669, 822
Hpy188III TCNNGA 3 cut(s) 332, 506, 905
Hpy8I GTNNAC 2 cut(s) 417, 774
HpyAV CCTTC 3 cut(s) 87, 417, 527
HpyCH4III ACNGT 3 cut(s) 286, 653, 891
HpyCH4V TGCA 8 cut(s) 57, 89, 182, 244, 250, 290, 550, 918
HpyF10VI GCNNNNNNNGC 6 cut(s) 173, 179, 247, 287, 462, 883
HpyF3I CTNAG 6 cut(s) 189, 223, 575, 666, 694, 803
Hsp92I GRCGYC 1 cut(s) 702
Hsp92II CATG 2 cut(s) 218, 709
KspAI GTTAAC 1 cut(s) 774
Kzo9I GATC 7 cut(s) 217, 268, 519, 601, 638, 760, 828
LmnI GCTCC 1 cut(s) 964
Lsp1109I GCAGC 5 cut(s) 154, 157, 160, 228, 302
LweI GCATC 5 cut(s) 154, 169, 238, 905, 916
MaeIII GTNAC 3 cut(s) 154, 537, 846
MalI GATC 7 cut(s) 219, 270, 521, 603, 640, 762, 830
MboI GATC 7 cut(s) 217, 268, 519, 601, 638, 760, 828
MboII GAAGA 3 cut(s) 471, 529, 920
MflI RGATCY 1 cut(s) 601
MlsI TGGCCA 2 cut(s) 584, 835
MluCI AATT 9 cut(s) 35, 60, 105, 336, 393, 432, 474, 500, 947
MluNI TGGCCA 2 cut(s) 584, 835
MlyI GAGTC 1 cut(s) 566
MmeI TCCRAC 1 cut(s) 335
MnlI CCTC 3 cut(s) 116, 629, 675
Mox20I TGGCCA 2 cut(s) 584, 835
MscI TGGCCA 2 cut(s) 584, 835
MseI TTAA 5 cut(s) 104, 690, 773, 780, 945
Msp20I TGGCCA 2 cut(s) 584, 835
MspA1I CMGCKG 2 cut(s) 151, 738
MspR9I CCNGG 3 cut(s) 235, 311, 581
MvaI CCWGG 3 cut(s) 235, 311, 581
MwoI GCNNNNNNNGC 6 cut(s) 173, 179, 247, 287, 462, 883
NdeII GATC 7 cut(s) 217, 268, 519, 601, 638, 760, 828
NlaIII CATG 2 cut(s) 218, 709
NlaIV GGNNCC 2 cut(s) 383, 727
NmuCI GTSAC 2 cut(s) 154, 846
NspV TTCGAA 1 cut(s) 498
PceI AGGCCT 1 cut(s) 126
PcsI WCGNNNNNNNCGW 3 cut(s) 504, 643, 708
PfeI GAWTC 5 cut(s) 83, 328, 370, 487, 741
PkrI GCNGC 5 cut(s) 169, 172, 175, 243, 292
PleI GAGTC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 565
Psp6I CCWGG 3 cut(s) 233, 309, 579
PspGI CCWGG 3 cut(s) 233, 309, 579
PspN4I GGNNCC 2 cut(s) 383, 727
PspPI GGNCC 2 cut(s) 382, 725
PstNI CAGNNNCTG 3 cut(s) 86, 167, 188
PsuI RGATCY 1 cut(s) 601
RsaI GTAC 2 cut(s) 147, 751
RsaNI GTAC 2 cut(s) 146, 750
SaqAI TTAA 5 cut(s) 104, 690, 773, 780, 945
SatI GCNGC 5 cut(s) 168, 171, 174, 242, 291
Sau3AI GATC 7 cut(s) 217, 268, 519, 601, 638, 760, 828
Sau96I GGNCC 2 cut(s) 382, 725
SchI GAGTC 1 cut(s) 566
ScrFI CCNGG 3 cut(s) 235, 311, 581
SetI ASST 9 cut(s) 243, 312, 369, 444, 458, 621, 879, 888, 961
SfaNI GCATC 5 cut(s) 154, 169, 238, 905, 916
SfcI CTRYAG 2 cut(s) 159, 282
SfuI TTCGAA 1 cut(s) 498
SinI GGWCC 1 cut(s) 382
SmlI CTYRAG 1 cut(s) 789
SmoI CTYRAG 1 cut(s) 789
Sse9I AATT 9 cut(s) 35, 60, 105, 336, 393, 432, 474, 500, 947
SseBI AGGCCT 1 cut(s) 126
SsiI CCGC 2 cut(s) 151, 738
StuI AGGCCT 1 cut(s) 126
StyD4I CCNGG 3 cut(s) 233, 309, 579
StyI CCWWGG 2 cut(s) 341, 566
TaaI ACNGT 3 cut(s) 286, 653, 891
TaqI TCGA 6 cut(s) 498, 507, 555, 637, 646, 711
TasI AATT 9 cut(s) 35, 60, 105, 336, 393, 432, 474, 500, 947
TatI WGTACW 1 cut(s) 145
TfiI GAWTC 5 cut(s) 83, 328, 370, 487, 741
Tru1I TTAA 5 cut(s) 104, 690, 773, 780, 945
Tru9I TTAA 5 cut(s) 104, 690, 773, 780, 945
TscAI CASTG 1 cut(s) 271
TseFI GTSAC 2 cut(s) 154, 846
TseI GCWGC 5 cut(s) 167, 170, 173, 241, 290
Tsp45I GTSAC 2 cut(s) 154, 846
TspDTI ATGAA 3 cut(s) 60, 483, 853
TspGWI ACGGA 2 cut(s) 402, 692
TspRI CASTG 1 cut(s) 271
VpaK11BI GGWCC 1 cut(s) 382
XapI RAATTY 3 cut(s) 336, 432, 500
XmiI GTMKAC 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.