MD07G1191500.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003808
Physical Location & Seq
Reverse (-)
27137243 .. 27138484
1242 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1191500.v1.1.491

Sequence Viewer

Length: 1014 bp
ATGGAGAAGGAAATAGCAGATGATTTGTTTGGCAAATTACCAAATGAAATCATTTGCAGAATTATTTCTTTTCTCCCTTCAGAATCTGCATTAGAAACCAGTTTAATCTCAAGTAGGTGGAGAGGCCTGTGGAATACAACTTTAGCGAAGCAGATCACTACAGATGATGATGCAGCTGATGCAGTTTCTGAATTTGTCTCTCACTTTAACCAGCTTGATCCCTTAGGGCATCCCAGAAAGCTGCAACTGCACTTTGGCAAACTTGGCGATCTCCTTTTGGCTACAGTTGCACCCAACAATAAACTCCGTCTGGAGTGTTCGACAGAGAATCGGGAAAATCCAAGGCATTTTGGTTGGCACTTGAAGCTGAATCACCAAAGGGACCAAACCCTAATTTCTCACCAGCCTTCTCCCTCTACTTTCTTTGTCAAAATTCTTTACCTAAAATCAGTGAGCTCTTTTGGCAATGAAGCAGTTTCTTCTATGATTCCAAGTTTCGAATATCTTGAAAAATTGAAGATCATTGAATGTGAGGGGTTACAATCTTTGCATATCGAGTCCAGTCCAAGGCTACTTAGCTTGACCATCTTTGACTGCCAGAATCTCAAGTCTCTCCACCTCAAAACTTCCAAACTTCGATCTTTTCGATACCGCGGTTTTGTCCCTCATATTTGGAGGGAACATCATTTTAACTTGGCAGACGCCATGCTTGATTTCAGGCAAGGCCCCGGCTACGGCGGATTCAAAAGTAGCGACTTTGATCCTATGTTGTTAACCATTAAAAATGCTCAAGTTCTCACACTATGCAAATGGACTTTCCAGACATTGATCTGGCCATCCCTTTTGCCTCTACAAGCAAACTTCATATTCTACAAGCTGAAAGAGCTATGGTGGATTGATAACTGGGAAGAAAGATTCAGCATTGATCCTCTAATATCCTTCATTAAATTATGTCCAGCTTTGGAGCAACTCTATATAACTGTAAGTTTCTTTCACCCAAAACTATACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBD

Protein Analysis

338

Amino Acids

39.1

Weight (kDa)

7.7

Isoelectric Point (pI)

39.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 145 - 329 1.2e-08 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 654
AciI CCGC 3 cut(s) 652, 654, 738
AclWI GGATC 3 cut(s) 212, 755, 920
AcoI YGGCCR 1 cut(s) 833
AcsI RAATTY 2 cut(s) 191, 432
AcuI CTGAAG 1 cut(s) 63
AcyI GRCGYC 1 cut(s) 702
AfiI CCNNNNNNNGG 2 cut(s) 567, 734
AgsI TTSAA 5 cut(s) 364, 509, 517, 527, 745
AluBI AGCT 9 cut(s) 176, 214, 241, 367, 456, 579, 877, 886, 959
AluI AGCT 9 cut(s) 176, 214, 241, 367, 456, 579, 877, 886, 959
Alw21I GWGCWC 1 cut(s) 458
Alw26I GTCTC 2 cut(s) 202, 615
AlwI GGATC 3 cut(s) 212, 755, 920
AlwNI CAGNNNCTG 2 cut(s) 86, 188
AoxI GGCC 3 cut(s) 124, 724, 833
ApeKI GCWGC 2 cut(s) 173, 241
ApoI RAATTY 2 cut(s) 191, 432
Asp700I GAANNNNTTC 1 cut(s) 64
AspS9I GGNCC 2 cut(s) 382, 725
AsuC2I CCSGG 1 cut(s) 729
AsuHPI GGTGA 3 cut(s) 365, 392, 986
AsuII TTCGAA 1 cut(s) 498
AvaII GGWCC 1 cut(s) 382
AxyI CCTNAGG 1 cut(s) 223
BaeI ACNNNNGTAYC 2 cut(s) 640, 673
BalI TGGCCA 1 cut(s) 835
BanII GRGCYC 1 cut(s) 458
Bbv12I GWGCWC 1 cut(s) 458
BbvI GCAGC 2 cut(s) 185, 228
BccI CCATC 2 cut(s) 593, 844
BceAI ACGGC 1 cut(s) 751
BcnI CCSGG 1 cut(s) 729
BcoDI GTCTC 2 cut(s) 202, 615
BfmI CTRYAG 2 cut(s) 159, 282
BisI GCNGC 2 cut(s) 174, 242
BlsI GCNGC 2 cut(s) 175, 243
Bme1390I CCNGG 1 cut(s) 729
Bme18I GGWCC 1 cut(s) 382
BmgT120I GGNCC 2 cut(s) 382, 725
BmiI GGNNCC 2 cut(s) 383, 727
BmrFI CCNGG 1 cut(s) 729
BmrI ACTGGG 1 cut(s) 913
BmsI GCATC 3 cut(s) 160, 169, 238
BmuI ACTGGG 1 cut(s) 913
BpmI CTGGAG 1 cut(s) 332
Bpu14I TTCGAA 1 cut(s) 498
BpuEI CTTGAG 3 cut(s) 94, 590, 774
BpuMI CCSGG 1 cut(s) 729
BsaHI GRCGYC 1 cut(s) 702
BsaJI CCNNGG 4 cut(s) 341, 566, 652, 727
Bsc4I CCNNNNNNNGG 2 cut(s) 567, 734
Bse1I ACTGG 3 cut(s) 99, 561, 908
Bse21I CCTNAGG 1 cut(s) 223
Bse3DI GCAATG 1 cut(s) 472
BseDI CCNNGG 4 cut(s) 341, 566, 652, 727
BseGI GGATG 2 cut(s) 229, 836
BseLI CCNNNNNNNGG 2 cut(s) 567, 734
BseMI GCAATG 1 cut(s) 472
BseNI ACTGG 3 cut(s) 99, 561, 908
BseXI GCAGC 2 cut(s) 185, 228
BsgI GTGCAG 1 cut(s) 233
Bsh1236I CGCG 1 cut(s) 654
BshFI GGCC 3 cut(s) 126, 726, 835
BsiHKAI GWGCWC 1 cut(s) 458
BsiSI CCGG 1 cut(s) 729
BslFI GGGAC 2 cut(s) 395, 647
BslI CCNNNNNNNGG 2 cut(s) 567, 734
BsmAI GTCTC 2 cut(s) 202, 615
BsmFI GGGAC 2 cut(s) 395, 647
BsnI GGCC 3 cut(s) 126, 726, 835
Bsp119I TTCGAA 1 cut(s) 498
Bsp1286I GDGCHC 1 cut(s) 458
Bsp143I GATC 8 cut(s) 153, 217, 268, 519, 638, 760, 828, 925
BspACI CCGC 3 cut(s) 652, 654, 738
BspANI GGCC 3 cut(s) 126, 726, 835
BspFNI CGCG 1 cut(s) 654
BspLI GGNNCC 2 cut(s) 383, 727
BspPI GGATC 3 cut(s) 212, 755, 920
BspT104I TTCGAA 1 cut(s) 498
BsrDI GCAATG 1 cut(s) 472
BsrI ACTGG 3 cut(s) 99, 561, 908
BssECI CCNNGG 4 cut(s) 341, 566, 652, 727
BssMI GATC 8 cut(s) 153, 217, 268, 519, 638, 760, 828, 925
BssNI GRCGYC 1 cut(s) 702
BssT1I CCWWGG 2 cut(s) 341, 566
Bst4CI ACNGT 2 cut(s) 286, 982
BstACI GRCGYC 1 cut(s) 702
BstAPI GCANNNNNTGC 1 cut(s) 179
BstBI TTCGAA 1 cut(s) 498
BstDEI CTNAG 2 cut(s) 223, 575
BstDSI CCRYGG 1 cut(s) 652
BstF5I GGATG 2 cut(s) 229, 836
BstFNI CGCG 1 cut(s) 654
BstKTI GATC 8 cut(s) 156, 220, 271, 522, 641, 763, 831, 928
BstMAI GTCTC 2 cut(s) 202, 615
BstMBI GATC 8 cut(s) 153, 217, 268, 519, 638, 760, 828, 925
BstMWI GCNNNNNNNGC 7 cut(s) 179, 247, 264, 287, 364, 462, 883
BstSCI CCNGG 1 cut(s) 727
BstSFI CTRYAG 2 cut(s) 159, 282
BstUI CGCG 1 cut(s) 654
BstV1I GCAGC 2 cut(s) 185, 228
Bsu36I CCTNAGG 1 cut(s) 223
BsuRI GGCC 3 cut(s) 126, 726, 835
BtgI CCRYGG 1 cut(s) 652
BtsCI GGATG 2 cut(s) 229, 836
BtsIMutI CAGTG 1 cut(s) 456
CaiI CAGNNNCTG 2 cut(s) 86, 188
Cfr13I GGNCC 2 cut(s) 382, 725
Cfr42I CCGCGG 1 cut(s) 655
CseI GACGC 1 cut(s) 710
CviAII CATG 1 cut(s) 706
DdeI CTNAG 2 cut(s) 223, 575
DpnI GATC 8 cut(s) 155, 219, 270, 521, 640, 762, 830, 927
DpnII GATC 8 cut(s) 153, 217, 268, 519, 638, 760, 828, 925
EaeI YGGCCR 1 cut(s) 833
EciI GGCGGA 1 cut(s) 753
Ecl136II GAGCTC 1 cut(s) 456
Eco130I CCWWGG 2 cut(s) 341, 566
Eco147I AGGCCT 1 cut(s) 126
Eco24I GRGCYC 1 cut(s) 458
Eco47I GGWCC 1 cut(s) 382
Eco53kI GAGCTC 1 cut(s) 456
Eco57I CTGAAG 1 cut(s) 63
Eco81I CCTNAGG 1 cut(s) 223
EcoICRI GAGCTC 1 cut(s) 456
EcoO109I RGGNCCY 1 cut(s) 725
EcoT14I CCWWGG 2 cut(s) 341, 566
EcoT38I GRGCYC 1 cut(s) 458
ErhI CCWWGG 2 cut(s) 341, 566
FaeI CATG 1 cut(s) 709
FaqI GGGAC 2 cut(s) 395, 647
FatI CATG 1 cut(s) 705
Fnu4HI GCNGC 2 cut(s) 174, 242
FokI GGATG 2 cut(s) 216, 823
FriOI GRGCYC 1 cut(s) 458
Fsp4HI GCNGC 2 cut(s) 174, 242
GluI GCNGC 2 cut(s) 174, 242
GsuI CTGGAG 1 cut(s) 332
HaeIII GGCC 3 cut(s) 126, 726, 835
HapII CCGG 1 cut(s) 729
HgaI GACGC 1 cut(s) 710
Hin1I GRCGYC 1 cut(s) 702
Hin1II CATG 1 cut(s) 709
HincII GTYRAC 1 cut(s) 774
HindII GTYRAC 1 cut(s) 774
HinfI GANTC 8 cut(s) 83, 328, 370, 487, 557, 601, 741, 915
HpaI GTTAAC 1 cut(s) 774
HpaII CCGG 1 cut(s) 729
HphI GGTGA 3 cut(s) 365, 392, 986
Hpy166II GTNNAC 1 cut(s) 774
Hpy188I TCNGA 2 cut(s) 82, 190
Hpy188III TCNNGA 4 cut(s) 311, 332, 506, 820
Hpy8I GTNNAC 1 cut(s) 774
HpyAV CCTTC 3 cut(s) 87, 417, 949
HpyCH4III ACNGT 2 cut(s) 286, 982
HpyCH4V TGCA 9 cut(s) 57, 89, 173, 182, 244, 250, 290, 550, 807
HpyF10VI GCNNNNNNNGC 7 cut(s) 179, 247, 264, 287, 364, 462, 883
HpyF3I CTNAG 2 cut(s) 223, 575
Hsp92I GRCGYC 1 cut(s) 702
Hsp92II CATG 1 cut(s) 709
KspAI GTTAAC 1 cut(s) 774
KspI CCGCGG 1 cut(s) 655
Kzo9I GATC 8 cut(s) 153, 217, 268, 519, 638, 760, 828, 925
LmnI GCTCC 1 cut(s) 964
Lsp1109I GCAGC 2 cut(s) 185, 228
LweI GCATC 3 cut(s) 160, 169, 238
MaeIII GTNAC 1 cut(s) 537
MalI GATC 8 cut(s) 155, 219, 270, 521, 640, 762, 830, 927
MboI GATC 8 cut(s) 153, 217, 268, 519, 638, 760, 828, 925
MboII GAAGA 3 cut(s) 471, 529, 920
MhlI GDGCHC 1 cut(s) 458
MlsI TGGCCA 1 cut(s) 835
MluCI AATT 7 cut(s) 35, 60, 191, 393, 432, 512, 947
MluNI TGGCCA 1 cut(s) 835
MlyI GAGTC 1 cut(s) 566
MnlI CCTC 8 cut(s) 116, 424, 526, 629, 669, 675, 858, 939
Mox20I TGGCCA 1 cut(s) 835
MroXI GAANNNNTTC 1 cut(s) 64
MscI TGGCCA 1 cut(s) 835
MseI TTAA 6 cut(s) 104, 207, 690, 773, 780, 945
Msp20I TGGCCA 1 cut(s) 835
MspA1I CMGCKG 2 cut(s) 176, 654
MspI CCGG 1 cut(s) 729
MspR9I CCNGG 1 cut(s) 729
MvnI CGCG 1 cut(s) 654
MwoI GCNNNNNNNGC 7 cut(s) 179, 247, 264, 287, 364, 462, 883
NciI CCSGG 1 cut(s) 729
NdeII GATC 8 cut(s) 153, 217, 268, 519, 638, 760, 828, 925
NlaIII CATG 1 cut(s) 709
NlaIV GGNNCC 2 cut(s) 383, 727
NspV TTCGAA 1 cut(s) 498
PceI AGGCCT 1 cut(s) 126
PcsI WCGNNNNNNNCGW 1 cut(s) 643
PdmI GAANNNNTTC 1 cut(s) 64
PfeI GAWTC 7 cut(s) 83, 328, 370, 487, 601, 741, 915
PkrI GCNGC 2 cut(s) 175, 243
PleI GAGTC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 565
Psp124BI GAGCTC 1 cut(s) 458
PspN4I GGNNCC 2 cut(s) 383, 727
PspPI GGNCC 2 cut(s) 382, 725
PstNI CAGNNNCTG 2 cut(s) 86, 188
PvuII CAGCTG 1 cut(s) 176
SacI GAGCTC 1 cut(s) 458
SacII CCGCGG 1 cut(s) 655
SaqAI TTAA 6 cut(s) 104, 207, 690, 773, 780, 945
SatI GCNGC 2 cut(s) 174, 242
Sau3AI GATC 8 cut(s) 153, 217, 268, 519, 638, 760, 828, 925
Sau96I GGNCC 2 cut(s) 382, 725
SchI GAGTC 1 cut(s) 566
ScrFI CCNGG 1 cut(s) 729
SduI GDGCHC 1 cut(s) 458
SfaNI GCATC 3 cut(s) 160, 169, 238
SfcI CTRYAG 2 cut(s) 159, 282
Sfr303I CCGCGG 1 cut(s) 655
SfuI TTCGAA 1 cut(s) 498
SgrBI CCGCGG 1 cut(s) 655
SinI GGWCC 1 cut(s) 382
SmlI CTYRAG 3 cut(s) 109, 605, 789
SmoI CTYRAG 3 cut(s) 109, 605, 789
Sse9I AATT 7 cut(s) 35, 60, 191, 393, 432, 512, 947
SseBI AGGCCT 1 cut(s) 126
SsiI CCGC 3 cut(s) 652, 654, 738
SstI GAGCTC 1 cut(s) 458
StuI AGGCCT 1 cut(s) 126
StyD4I CCNGG 1 cut(s) 727
StyI CCWWGG 2 cut(s) 341, 566
TaaI ACNGT 2 cut(s) 286, 982
TaqI TCGA 5 cut(s) 320, 498, 555, 637, 646
TasI AATT 7 cut(s) 35, 60, 191, 393, 432, 512, 947
TfiI GAWTC 7 cut(s) 83, 328, 370, 487, 601, 741, 915
Tru1I TTAA 6 cut(s) 104, 207, 690, 773, 780, 945
Tru9I TTAA 6 cut(s) 104, 207, 690, 773, 780, 945
TscAI CASTG 1 cut(s) 456
TseI GCWGC 2 cut(s) 173, 241
TspDTI ATGAA 4 cut(s) 60, 483, 853, 931
TspGWI ACGGA 1 cut(s) 296
TspRI CASTG 1 cut(s) 456
VpaK11BI GGWCC 1 cut(s) 382
XapI RAATTY 2 cut(s) 191, 432
XmnI GAANNNNTTC 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.