MD01G1202100.v1.1

DNA-directed RNA polymerase III subunit

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
Physical Location & Seq
Forward (+)
29806495 .. 29810256
3762 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1202100.v1.1.491

Sequence Viewer

Length: 1521 bp
ATGCCGGCCACCCACTTCGGCGTCAAGTACGCCGTACACATCATCACCTCCCACTTCGGCAATCTCGTCGCTAAAGTGTGTGAAAGTCTTCTCAAAAGAGGACCCCTAACCCTAAAAATCCTAATTGGGTTCACGGAGCTCACCCCTCAGCAAGTCAAGAACTCCCTCCTTATCCTAATCCAGCACAACAGCGTTCAGCCCTTCTCCGTAGACCAAGCAGAGGGTCCGAAATTCACTCAGTATGTAGCGCTGTTCGATAACATACTCCATCGAGGGAGGTTTTCCAAATTCATGACGGTTGTTTCGCAGGAGTTTGATAAAGAGTGTGAACTGGTTATTAAGGATTTGCTTGAGAATGGTAGGCTTACACTAAAACAAATAACTGAAGGAGAATATGCAGTTAAGGATGCTGTTGAGAAAAGCTTTCTTAAACTTGTGGACGCTCGTTTTGTGGAATGCTGCCCGGCTCCTGAAGTAGTTCTTGAAGAAGCAAGTAAAGACGCTCCAAAGAAGCGAGGTCCTAAGTTTTCTAAGATTGTTGAAGTACCAGAGACAATAGAACAACGTGTTGTAGCAGCAGCAGCACCTTCAGGAGCTATGAGATTTTCAATCCTGACAGATTCTGAAACCGATGCTCATGGGGGTAGTATGAGTACCGGGGACAAGCGCAAGCATGATGCTTTGGAGTTGGATGAAGAATTTGCTAATAATGAAATGGTTCTTTGGCGTGTTAATTTTGAGGAATTCATTCGCTGTCTAAGGCACAAGGTTTGCGTTGAGAATGTGAGAGCACGTCTGGATGATGGAGCTGCACTTGTCTTAAGGGCAATGCTTAAGTCAACTAGAACTGCAGAAAAGAAAGTAAAGACAGAAAATTCAGTTCCTGTATCATTTAGTACCATTTATGAGGAGGTGATAAACAGTGAAGCTGGTCGTAATTTGACATGTGATCGCGTCAAAGCTTCCCTCAGCCTGTTGTGCGATGAAAGGGATGTAGATGAGGATGAGTTGTATAGTGTTGACTTGAAGAAAATTCTTGAGCTGGCTCGGAATGATGAGGTGGAGTCAATTGTTTTGAAAAGATTTGGGAGAGATGCTTATAAAATATTTCGGCTACTGTCCACTTCAAGTCGTGCAATTGAGACGGATAAGATTTCAGATATGACACTCGTTGAAAAGAAGGAAACTCCCACAATTCTGTATAAGCTGTGGAAGGATGAGTACTTGCTTATGGAGAGATTAGCTGTGACGGGAGCCAGACAGGCACAATTCTTGGTGTGGAAGGTAAACAAGCCCATTGTATGGAAACATGTTTTAGACGAGATGTTCCACGCTGCGCTGAATCTGACTCTTCGACTGGCATACGAGGATGAACAAATTAAAGAGGTACAACTTCTCCCGGCGCACAAGCGAACGGGGCCATTAGGGAAACAATGGCAACGATTTCGAAATGTGGTTTATGTTATGCACGCAACCCTTTTGAAGATTGATGATGCTATCATGCTCTTCCATGACTTCTGA

Protein Analysis

507

Amino Acids

57.7

Weight (kDa)

6.53

Isoelectric Point (pI)

36.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HTH_9 PF08221 10 - 66 5.9e-18 RNA polymerase III subunit RPC82 helix-turn-helix domain
RNA_pol_Rpc82 PF05645 140 - 315 3e-21 RNA polymerase III subunit RPC82
WHD_POLR3C PF22536 354 - 419 3.9e-11 POLR3C, C-terminal winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1101
AccB7I CCANNNNNTGG 1 cut(s) 1302
AccI GTMKAC 1 cut(s) 210
AccII CGCG 1 cut(s) 954
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 6 cut(s) 230, 287, 698, 743, 874, 1032
AcuI CTGAAG 3 cut(s) 405, 492, 573
AcyI GRCGYC 1 cut(s) 21
AfaI GTAC 7 cut(s) 29, 36, 546, 655, 898, 1223, 1389
AfeI AGCGCT 1 cut(s) 249
AfiI CCNNNNNNNGG 2 cut(s) 220, 1302
AflII CTTAAG 2 cut(s) 820, 833
AflIII ACRYGT 3 cut(s) 565, 944, 1309
AgsI TTSAA 8 cut(s) 485, 542, 609, 1027, 1078, 1128, 1175, 1483
AjiI CACGTC 1 cut(s) 794
AluBI AGCT 9 cut(s) 139, 423, 596, 809, 929, 962, 1042, 1207, 1244
AluI AGCT 9 cut(s) 139, 423, 596, 809, 929, 962, 1042, 1207, 1244
Alw21I GWGCWC 2 cut(s) 141, 793
Alw26I GTCTC 2 cut(s) 545, 1136
AlwNI CAGNNNCTG 2 cut(s) 623, 884
Aor51HI AGCGCT 1 cut(s) 249
AoxI GGCC 2 cut(s) 6, 1418
ApeKI GCWGC 6 cut(s) 459, 575, 578, 581, 809, 1334
ApoI RAATTY 6 cut(s) 230, 287, 698, 743, 874, 1032
ArsI GACNNNNNNTTYG 4 cut(s) 286, 318, 431, 463
Asp700I GAANNNNTTC 4 cut(s) 87, 477, 717, 747
AspLEI GCGC 4 cut(s) 250, 669, 1339, 1405
AspS9I GGNCC 4 cut(s) 101, 224, 518, 1418
AsuC2I CCSGG 3 cut(s) 464, 658, 1400
AsuHPI GGTGA 3 cut(s) 37, 133, 925
AsuII TTCGAA 1 cut(s) 1447
AvaII GGWCC 3 cut(s) 101, 224, 518
BanII GRGCYC 1 cut(s) 141
BarI GAAGNNNNNNTAC 2 cut(s) 1205, 1237
BbsI GAAGAC 1 cut(s) 80
Bbv12I GWGCWC 2 cut(s) 141, 793
BbvCI CCTCAGC 2 cut(s) 147, 968
BbvI GCAGC 6 cut(s) 446, 587, 590, 593, 796, 1321
BccI CCATC 2 cut(s) 276, 797
BceAI ACGGC 1 cut(s) 17
BcgI CGANNNNNNTGC 4 cut(s) 49, 83, 1427, 1461
BcnI CCSGG 3 cut(s) 464, 658, 1400
BcoDI GTCTC 2 cut(s) 545, 1136
BfaI CTAG 1 cut(s) 843
BfmI CTRYAG 1 cut(s) 849
BfoI RGCGCY 1 cut(s) 251
BfrI CTTAAG 2 cut(s) 820, 833
BglI GCCNNNNNGGC 1 cut(s) 1262
BisI GCNGC 6 cut(s) 460, 576, 579, 582, 810, 1335
BlsI GCNGC 6 cut(s) 461, 577, 580, 583, 811, 1336
BmcAI AGTACT 1 cut(s) 1223
Bme1390I CCNGG 3 cut(s) 464, 658, 1400
Bme18I GGWCC 3 cut(s) 101, 224, 518
BmgBI CACGTC 1 cut(s) 794
BmgT120I GGNCC 4 cut(s) 101, 224, 518, 1418
BmiI GGNNCC 5 cut(s) 103, 225, 468, 1255, 1419
BmrFI CCNGG 3 cut(s) 464, 658, 1400
BmsI GCATC 5 cut(s) 397, 622, 667, 1084, 1483
BpiI GAAGAC 1 cut(s) 80
Bpu10I CCTNAGC 2 cut(s) 147, 968
Bpu14I TTCGAA 1 cut(s) 1447
BpuEI CTTGAG 2 cut(s) 371, 1058
BpuMI CCSGG 3 cut(s) 464, 658, 1400
BsaHI GRCGYC 1 cut(s) 21
BsaJI CCNNGG 1 cut(s) 657
BsaXI ACNNNNNCTCC 2 cut(s) 1380, 1410
Bsc4I CCNNNNNNNGG 2 cut(s) 220, 1302
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 2 cut(s) 336, 1362
Bse3DI GCAATG 1 cut(s) 834
BseDI CCNNGG 1 cut(s) 657
BseGI GGATG 7 cut(s) 412, 697, 805, 997, 1009, 1222, 1375
BseLI CCNNNNNNNGG 2 cut(s) 220, 1302
BseMI GCAATG 1 cut(s) 834
BseMII CTCAG 3 cut(s) 161, 251, 982
BseNI ACTGG 2 cut(s) 336, 1362
BseRI GAGGAG 1 cut(s) 923
BseXI GCAGC 6 cut(s) 446, 587, 590, 593, 796, 1321
BsgI GTGCAG 1 cut(s) 795
Bsh1236I CGCG 1 cut(s) 954
BshFI GGCC 2 cut(s) 8, 1420
BsiHKAI GWGCWC 2 cut(s) 141, 793
BsiSI CCGG 4 cut(s) 5, 464, 657, 1400
BslFI GGGAC 1 cut(s) 674
BslI CCNNNNNNNGG 2 cut(s) 220, 1302
BsmAI GTCTC 2 cut(s) 545, 1136
BsmBI CGTCTC 1 cut(s) 1136
BsmFI GGGAC 1 cut(s) 674
BsmI GAATGC 1 cut(s) 461
BsnI GGCC 2 cut(s) 8, 1420
Bsp119I TTCGAA 1 cut(s) 1447
Bsp1286I GDGCHC 2 cut(s) 141, 793
Bsp143I GATC 1 cut(s) 949
BspANI GGCC 2 cut(s) 8, 1420
BspCNI CTCAG 3 cut(s) 160, 250, 981
BspFNI CGCG 1 cut(s) 954
BspHI TCATGA 1 cut(s) 291
BspLI GGNNCC 5 cut(s) 103, 225, 468, 1255, 1419
BspMAI CTGCAG 1 cut(s) 853
BspQI GCTCTTC 1 cut(s) 1511
BspT104I TTCGAA 1 cut(s) 1447
BspTI CTTAAG 2 cut(s) 820, 833
BsrDI GCAATG 1 cut(s) 834
BsrFI RCCGGY 1 cut(s) 4
BsrI ACTGG 2 cut(s) 336, 1362
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 1 cut(s) 657
BssMI GATC 1 cut(s) 949
BssNI GRCGYC 1 cut(s) 21
Bst4CI ACNGT 3 cut(s) 298, 923, 1119
Bst6I CTCTTC 2 cut(s) 1356, 1511
BstACI GRCGYC 1 cut(s) 21
BstAFI CTTAAG 2 cut(s) 820, 833
BstBI TTCGAA 1 cut(s) 1447
BstC8I GCNNGC 4 cut(s) 6, 671, 1044, 1470
BstDEI CTNAG 6 cut(s) 147, 237, 522, 531, 758, 968
BstF5I GGATG 7 cut(s) 412, 697, 805, 997, 1009, 1222, 1375
BstFNI CGCG 1 cut(s) 954
BstH2I RGCGCY 1 cut(s) 251
BstHHI GCGC 4 cut(s) 250, 669, 1339, 1405
BstKTI GATC 1 cut(s) 952
BstMAI GTCTC 2 cut(s) 545, 1136
BstMBI GATC 1 cut(s) 949
BstMWI GCNNNNNNNGC 4 cut(s) 581, 978, 1262, 1417
BstNSI RCATGY 2 cut(s) 948, 1313
BstSCI CCNGG 3 cut(s) 462, 656, 1398
BstSFI CTRYAG 1 cut(s) 849
BstUI CGCG 1 cut(s) 954
BstV1I GCAGC 6 cut(s) 446, 587, 590, 593, 796, 1321
BstV2I GAAGAC 1 cut(s) 80
BsuRI GGCC 2 cut(s) 8, 1420
BtgZI GCGATG 1 cut(s) 996
BtrI CACGTC 1 cut(s) 794
BtsCI GGATG 7 cut(s) 412, 697, 805, 997, 1009, 1222, 1375
BtsIMutI CAGTG 1 cut(s) 928
Cac8I GCNNGC 4 cut(s) 6, 671, 1044, 1470
CaiI CAGNNNCTG 2 cut(s) 623, 884
CciI TCATGA 1 cut(s) 291
CfoI GCGC 4 cut(s) 250, 669, 1339, 1405
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 4 cut(s) 101, 224, 518, 1418
CseI GACGC 4 cut(s) 10, 449, 509, 943
Csp6I GTAC 7 cut(s) 28, 35, 545, 654, 897, 1222, 1388
CviAII CATG 7 cut(s) 292, 638, 674, 945, 1310, 1501, 1511
CviQI GTAC 7 cut(s) 28, 35, 545, 654, 897, 1222, 1388
DdeI CTNAG 6 cut(s) 147, 237, 522, 531, 758, 968
DpnI GATC 1 cut(s) 951
DpnII GATC 1 cut(s) 949
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 2 cut(s) 1356, 1511
EarI CTCTTC 2 cut(s) 1356, 1511
Ecl136II GAGCTC 1 cut(s) 139
Eco24I GRGCYC 1 cut(s) 141
Eco47I GGWCC 3 cut(s) 101, 224, 518
Eco47III AGCGCT 1 cut(s) 249
Eco53kI GAGCTC 1 cut(s) 139
Eco57I CTGAAG 3 cut(s) 405, 492, 573
EcoICRI GAGCTC 1 cut(s) 139
EcoO109I RGGNCCY 2 cut(s) 101, 518
EcoRI GAATTC 1 cut(s) 743
EcoT38I GRGCYC 1 cut(s) 141
Esp3I CGTCTC 1 cut(s) 1136
FaeI CATG 7 cut(s) 295, 641, 677, 948, 1313, 1504, 1514
FalI AAGNNNNNCTT 2 cut(s) 465, 497
FaqI GGGAC 1 cut(s) 674
FatI CATG 7 cut(s) 291, 637, 673, 944, 1309, 1500, 1510
FblI GTMKAC 1 cut(s) 210
Fnu4HI GCNGC 6 cut(s) 460, 576, 579, 582, 810, 1335
FokI GGATG 7 cut(s) 419, 704, 812, 1004, 1016, 1229, 1382
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 6 cut(s) 460, 576, 579, 582, 810, 1335
FspBI CTAG 1 cut(s) 843
GlaI GCGC 4 cut(s) 249, 668, 1338, 1404
GluI GCNGC 6 cut(s) 460, 576, 579, 582, 810, 1335
HaeII RGCGCY 1 cut(s) 251
HaeIII GGCC 2 cut(s) 8, 1420
HapII CCGG 4 cut(s) 5, 464, 657, 1400
HgaI GACGC 4 cut(s) 10, 449, 509, 943
HhaI GCGC 4 cut(s) 250, 669, 1339, 1405
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 7 cut(s) 295, 641, 677, 948, 1313, 1504, 1514
Hin6I GCGC 4 cut(s) 248, 667, 1337, 1403
HinP1I GCGC 4 cut(s) 248, 667, 1337, 1403
HincII GTYRAC 2 cut(s) 840, 1021
HindII GTYRAC 2 cut(s) 840, 1021
HindIII AAGCTT 2 cut(s) 421, 960
HinfI GANTC 4 cut(s) 620, 1064, 1342, 1348
HpaII CCGG 4 cut(s) 5, 464, 657, 1400
HphI GGTGA 3 cut(s) 37, 133, 925
Hpy166II GTNNAC 9 cut(s) 37, 132, 211, 329, 439, 840, 1021, 1122, 1288
Hpy188I TCNGA 6 cut(s) 228, 625, 1050, 1159, 1347, 1520
Hpy188III TCNNGA 8 cut(s) 157, 292, 470, 482, 591, 613, 797, 1037
Hpy8I GTNNAC 9 cut(s) 37, 132, 211, 329, 439, 840, 1021, 1122, 1288
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 6 cut(s) 211, 380, 597, 1174, 1207, 1276
HpyCH4III ACNGT 3 cut(s) 298, 923, 1119
HpyCH4IV ACGT 2 cut(s) 565, 793
HpyCH4V TGCA 5 cut(s) 398, 812, 851, 1136, 1468
HpyF10VI GCNNNNNNNGC 4 cut(s) 581, 978, 1262, 1417
HpyF3I CTNAG 6 cut(s) 147, 237, 522, 531, 758, 968
HpySE526I ACGT 2 cut(s) 565, 793
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 7 cut(s) 295, 641, 677, 948, 1313, 1504, 1514
HspAI GCGC 4 cut(s) 248, 667, 1337, 1403
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Kzo9I GATC 1 cut(s) 949
LguI GCTCTTC 1 cut(s) 1511
LmnI GCTCC 6 cut(s) 136, 472, 508, 593, 806, 1253
Lsp1109I GCAGC 6 cut(s) 446, 587, 590, 593, 796, 1321
LweI GCATC 5 cut(s) 397, 622, 667, 1084, 1483
MaeI CTAG 1 cut(s) 843
MaeII ACGT 2 cut(s) 565, 793
MaeIII GTNAC 1 cut(s) 1246
MalI GATC 1 cut(s) 951
MboI GATC 1 cut(s) 949
MboII GAAGA 7 cut(s) 80, 497, 707, 1039, 1343, 1495, 1498
MfeI CAATTG 2 cut(s) 1068, 1137
MhlI GDGCHC 2 cut(s) 141, 793
MlyI GAGTC 2 cut(s) 1073, 1342
MmeI TCCRAC 1 cut(s) 669
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 4 cut(s) 87, 477, 717, 747
MseI TTAA 7 cut(s) 339, 402, 429, 732, 821, 834, 1380
MspCI CTTAAG 2 cut(s) 820, 833
MspI CCGG 4 cut(s) 5, 464, 657, 1400
MspR9I CCNGG 3 cut(s) 464, 658, 1400
MunI CAATTG 2 cut(s) 1068, 1137
Mva1269I GAATGC 1 cut(s) 461
MvnI CGCG 1 cut(s) 954
MwoI GCNNNNNNNGC 4 cut(s) 581, 978, 1262, 1417
NaeI GCCGGC 1 cut(s) 6
NciI CCSGG 3 cut(s) 464, 658, 1400
NdeII GATC 1 cut(s) 949
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 7 cut(s) 295, 641, 677, 948, 1313, 1504, 1514
NlaIV GGNNCC 5 cut(s) 103, 225, 468, 1255, 1419
NmuCI GTSAC 1 cut(s) 1246
NspI RCATGY 2 cut(s) 948, 1313
NspV TTCGAA 1 cut(s) 1447
PagI TCATGA 1 cut(s) 291
PciI ACATGT 2 cut(s) 944, 1309
PciSI GCTCTTC 1 cut(s) 1511
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PctI GAATGC 1 cut(s) 461
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 4 cut(s) 87, 477, 717, 747
PfeI GAWTC 2 cut(s) 620, 1342
PflMI CCANNNNNTGG 1 cut(s) 1302
PkrI GCNGC 6 cut(s) 461, 577, 580, 583, 811, 1336
PleI GAGTC 2 cut(s) 1072, 1342
PpsI GAGTC 2 cut(s) 1072, 1342
PpuMI RGGWCCY 2 cut(s) 101, 518
PscI ACATGT 2 cut(s) 944, 1309
PsiI TTATAA 1 cut(s) 1101
Psp124BI GAGCTC 1 cut(s) 141
Psp5II RGGWCCY 2 cut(s) 101, 518
PspN4I GGNNCC 5 cut(s) 103, 225, 468, 1255, 1419
PspPI GGNCC 4 cut(s) 101, 224, 518, 1418
PspPPI RGGWCCY 2 cut(s) 101, 518
PstI CTGCAG 1 cut(s) 853
PstNI CAGNNNCTG 2 cut(s) 623, 884
RsaI GTAC 7 cut(s) 29, 36, 546, 655, 898, 1223, 1389
RsaNI GTAC 7 cut(s) 28, 35, 545, 654, 897, 1222, 1388
SacI GAGCTC 1 cut(s) 141
SapI GCTCTTC 1 cut(s) 1511
SaqAI TTAA 7 cut(s) 339, 402, 429, 732, 821, 834, 1380
SatI GCNGC 6 cut(s) 460, 576, 579, 582, 810, 1335
Sau3AI GATC 1 cut(s) 949
Sau96I GGNCC 4 cut(s) 101, 224, 518, 1418
ScaI AGTACT 1 cut(s) 1223
SchI GAGTC 2 cut(s) 1073, 1342
ScrFI CCNGG 3 cut(s) 464, 658, 1400
SduI GDGCHC 2 cut(s) 141, 793
SfaNI GCATC 5 cut(s) 397, 622, 667, 1084, 1483
SfcI CTRYAG 1 cut(s) 849
SfuI TTCGAA 1 cut(s) 1447
SinI GGWCC 3 cut(s) 101, 224, 518
SmlI CTYRAG 4 cut(s) 350, 820, 833, 1037
SmoI CTYRAG 4 cut(s) 350, 820, 833, 1037
SspI AATATT 1 cut(s) 1107
SspMI CTAG 1 cut(s) 843
SstI GAGCTC 1 cut(s) 141
StyD4I CCNGG 3 cut(s) 462, 656, 1398
TaaI ACNGT 3 cut(s) 298, 923, 1119
TaiI ACGT 2 cut(s) 568, 796
TaqI TCGA 4 cut(s) 255, 271, 1354, 1447
TatI WGTACW 1 cut(s) 1221
TfiI GAWTC 2 cut(s) 620, 1342
Tru1I TTAA 7 cut(s) 339, 402, 429, 732, 821, 834, 1380
Tru9I TTAA 7 cut(s) 339, 402, 429, 732, 821, 834, 1380
TscAI CASTG 1 cut(s) 928
TseFI GTSAC 1 cut(s) 1246
TseI GCWGC 6 cut(s) 459, 575, 578, 581, 809, 1334
Tsp45I GTSAC 1 cut(s) 1246
TspDTI ATGAA 6 cut(s) 280, 708, 726, 736, 999, 1386
TspGWI ACGGA 3 cut(s) 149, 196, 1160
TspRI CASTG 1 cut(s) 928
Van91I CCANNNNNTGG 1 cut(s) 1302
Vha464I CTTAAG 2 cut(s) 820, 833
VpaK11BI GGWCC 3 cut(s) 101, 224, 518
XapI RAATTY 6 cut(s) 230, 287, 698, 743, 874, 1032
XceI RCATGY 2 cut(s) 948, 1313
XmiI GTMKAC 1 cut(s) 210
XmnI GAANNNNTTC 4 cut(s) 87, 477, 717, 747
XspI CTAG 1 cut(s) 843
ZrmI AGTACT 1 cut(s) 1223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.