Rorug05G0583700

DNA-directed RNA polymerase III subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002788
Physical Location & Seq
Reverse (-)
78070673 .. 78073684
3012 bp
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UTR
Exon/CDS
Intron
Rorug05G0583700.1

Sequence Viewer

Length: 540 bp
ATGGCTTCCTCTTCTTCTTCTCATCTCTCAGCCATACCGCAACGCCTGCCTAAATCCTCACCAGGCCACTTCAACGCAAAGTCAGTTGGGTTTCATGTAAGATCCAATCTTGAGAACGAGAGTGGCAGTCCAGGGGATTCGGAGCATTCAGCTAAACCATCAAAGGGATCTCAGGTCTCAATCTCTCGACGATTGTGTCTCACATGTCTATGTTCAACTGCTGCTTTGATAAGCAACTCTCCAAAGGCAGTTGCACTGGATGGAAAAGACAGACCTCCTGGGTGCAGGAATTGTGGGGGAAGTGGAGCTGTGCTCTGTGATATGTGTGGTGGTACTGGAAAATGGAAAGCTCTCAACAGAAAACGAGCTAAAGATGTGTATGAGTTTACAGAATGTCCAAATTGTTACGGTCGGGGGAAACTTGTATGTCCTGTATGTTTAGGGACTGGTGTACCAAACAACAAAGGTCTTCTCAGGAGGCCTGATGCAAAGCAGCTACTTGAGAAGATGTACAATGGTCGGCTAGTGCCAAACTCTTAA

Protein Analysis

179

Amino Acids

18.95

Weight (kDa)

9.54

Isoelectric Point (pI)

56.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ_CXXCXGXG PF00684 95 - 150 3e-06 DnaJ central domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 195
AciI CCGC 1 cut(s) 38
AclWI GGATC 2 cut(s) 96, 175
AfaI GTAC 3 cut(s) 334, 453, 512
AfiI CCNNNNNNNGG 1 cut(s) 164
AflIII ACRYGT 1 cut(s) 203
AgsI TTSAA 2 cut(s) 73, 216
AjnI CCWGG 3 cut(s) 61, 130, 277
AluBI AGCT 5 cut(s) 152, 308, 350, 368, 496
AluI AGCT 5 cut(s) 152, 308, 350, 368, 496
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 2 cut(s) 181, 203
AlwI GGATC 2 cut(s) 96, 175
AoxI GGCC 2 cut(s) 64, 479
ApeKI GCWGC 2 cut(s) 221, 493
AsuHPI GGTGA 1 cut(s) 51
BaeI ACNNNNGTAYC 2 cut(s) 435, 468
BbsI GAAGAC 1 cut(s) 461
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 2 cut(s) 208, 505
BccI CCATC 2 cut(s) 166, 254
BciT130I CCWGG 3 cut(s) 63, 132, 279
BcoDI GTCTC 2 cut(s) 181, 203
BfaI CTAG 1 cut(s) 524
BisI GCNGC 2 cut(s) 222, 494
BlsI GCNGC 2 cut(s) 223, 495
Bme1390I CCNGG 3 cut(s) 63, 132, 279
BmrFI CCNGG 3 cut(s) 63, 132, 279
BmsI GCATC 1 cut(s) 475
BpiI GAAGAC 1 cut(s) 461
BplI GAGNNNNNCTC 2 cut(s) 297, 329
BpuEI CTTGAG 2 cut(s) 131, 521
BsaI GGTCTC 1 cut(s) 181
BsaJI CCNNGG 2 cut(s) 131, 278
Bsc4I CCNNNNNNNGG 1 cut(s) 164
Bse1I ACTGG 3 cut(s) 261, 340, 451
BseBI CCWGG 3 cut(s) 63, 132, 279
BseDI CCNNGG 2 cut(s) 131, 278
BseGI GGATG 1 cut(s) 265
BseLI CCNNNNNNNGG 1 cut(s) 164
BseMII CTCAG 3 cut(s) 42, 185, 487
BseNI ACTGG 3 cut(s) 261, 340, 451
BseXI GCAGC 2 cut(s) 208, 505
BsgI GTGCAG 1 cut(s) 304
Bsh1285I CGRYCG 1 cut(s) 412
BshFI GGCC 2 cut(s) 66, 481
BsiEI CGRYCG 1 cut(s) 412
BsiHKAI GWGCWC 1 cut(s) 315
BslFI GGGAC 1 cut(s) 457
BslI CCNNNNNNNGG 1 cut(s) 164
BsmAI GTCTC 2 cut(s) 181, 203
BsmFI GGGAC 1 cut(s) 457
BsmI GAATGC 1 cut(s) 145
BsnI GGCC 2 cut(s) 66, 481
Bso31I GGTCTC 1 cut(s) 181
Bsp1286I GDGCHC 1 cut(s) 315
Bsp1407I TGTACA 1 cut(s) 510
Bsp143I GATC 2 cut(s) 101, 167
BspACI CCGC 1 cut(s) 38
BspANI GGCC 2 cut(s) 66, 481
BspCNI CTCAG 3 cut(s) 41, 184, 486
BspPI GGATC 2 cut(s) 96, 175
BspTNI GGTCTC 1 cut(s) 181
BsrGI TGTACA 1 cut(s) 510
BsrI ACTGG 3 cut(s) 261, 340, 451
BssECI CCNNGG 2 cut(s) 131, 278
BssMI GATC 2 cut(s) 101, 167
Bst2UI CCWGG 3 cut(s) 63, 132, 279
Bst4CI ACNGT 1 cut(s) 410
Bst6I CTCTTC 1 cut(s) 16
BstAPI GCANNNNNTGC 1 cut(s) 46
BstAUI TGTACA 1 cut(s) 510
BstC8I GCNNGC 1 cut(s) 47
BstDEI CTNAG 3 cut(s) 28, 171, 473
BstF5I GGATG 1 cut(s) 265
BstKTI GATC 2 cut(s) 104, 170
BstMAI GTCTC 2 cut(s) 181, 203
BstMBI GATC 2 cut(s) 101, 167
BstMCI CGRYCG 1 cut(s) 412
BstMWI GCNNNNNNNGC 1 cut(s) 46
BstNI CCWGG 3 cut(s) 63, 132, 279
BstNSI RCATGY 1 cut(s) 207
BstSCI CCNGG 3 cut(s) 61, 130, 277
BstV1I GCAGC 2 cut(s) 208, 505
BstV2I GAAGAC 1 cut(s) 461
BstX2I RGATCY 2 cut(s) 101, 167
BstYI RGATCY 2 cut(s) 101, 167
BsuRI GGCC 2 cut(s) 66, 481
BtsCI GGATG 1 cut(s) 265
BtsIMutI CAGTG 1 cut(s) 254
Cac8I GCNNGC 1 cut(s) 47
Csp6I GTAC 3 cut(s) 333, 452, 511
CviAII CATG 2 cut(s) 95, 204
CviQI GTAC 3 cut(s) 333, 452, 511
DdeI CTNAG 3 cut(s) 28, 171, 473
DpnI GATC 2 cut(s) 103, 169
DpnII GATC 2 cut(s) 101, 167
DrdI GACNNNNNNGTC 1 cut(s) 195
DseDI GACNNNNNNGTC 1 cut(s) 195
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
Eco147I AGGCCT 1 cut(s) 481
Eco31I GGTCTC 1 cut(s) 181
EcoRII CCWGG 3 cut(s) 61, 130, 277
FaeI CATG 2 cut(s) 98, 207
FaiI YATR 8 cut(s) 35, 96, 205, 211, 323, 381, 427, 436
FaqI GGGAC 1 cut(s) 457
FatI CATG 2 cut(s) 94, 203
Fnu4HI GCNGC 2 cut(s) 222, 494
FokI GGATG 1 cut(s) 272
Fsp4HI GCNGC 2 cut(s) 222, 494
FspBI CTAG 1 cut(s) 524
GluI GCNGC 2 cut(s) 222, 494
HaeIII GGCC 2 cut(s) 66, 481
Hin1II CATG 2 cut(s) 98, 207
HinfI GANTC 1 cut(s) 137
HphI GGTGA 1 cut(s) 51
Hpy166II GTNNAC 2 cut(s) 387, 452
Hpy188I TCNGA 1 cut(s) 142
Hpy188III TCNNGA 3 cut(s) 110, 186, 475
Hpy8I GTNNAC 2 cut(s) 387, 452
Hpy99I CGWCG 1 cut(s) 192
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4V TGCA 3 cut(s) 254, 285, 488
HpyF10VI GCNNNNNNNGC 1 cut(s) 46
HpyF3I CTNAG 3 cut(s) 28, 171, 473
Hsp92II CATG 2 cut(s) 98, 207
Kzo9I GATC 2 cut(s) 101, 167
LmnI GCTCC 2 cut(s) 142, 305
Lsp1109I GCAGC 2 cut(s) 208, 505
LweI GCATC 1 cut(s) 475
MaeI CTAG 1 cut(s) 524
MaeIII GTNAC 1 cut(s) 404
MalI GATC 2 cut(s) 103, 169
MboI GATC 2 cut(s) 101, 167
MboII GAAGA 5 cut(s) 3, 6, 9, 461, 517
MflI RGATCY 2 cut(s) 101, 167
MhlI GDGCHC 1 cut(s) 315
MluCI AATT 2 cut(s) 289, 400
MnlI CCTC 4 cut(s) 19, 67, 285, 471
MseI TTAA 1 cut(s) 538
MslI CAYNNNNRTG 1 cut(s) 208
MspR9I CCNGG 3 cut(s) 63, 132, 279
Mva1269I GAATGC 1 cut(s) 145
MvaI CCWGG 3 cut(s) 63, 132, 279
MwoI GCNNNNNNNGC 1 cut(s) 46
NdeII GATC 2 cut(s) 101, 167
NlaIII CATG 2 cut(s) 98, 207
NspI RCATGY 1 cut(s) 207
PceI AGGCCT 1 cut(s) 481
PciI ACATGT 1 cut(s) 203
PctI GAATGC 1 cut(s) 145
PfeI GAWTC 1 cut(s) 137
PkrI GCNGC 2 cut(s) 223, 495
PscI ACATGT 1 cut(s) 203
Psp6I CCWGG 3 cut(s) 61, 130, 277
PspGI CCWGG 3 cut(s) 61, 130, 277
PsuI RGATCY 2 cut(s) 101, 167
RsaI GTAC 3 cut(s) 334, 453, 512
RsaNI GTAC 3 cut(s) 333, 452, 511
RseI CAYNNNNRTG 1 cut(s) 208
SaqAI TTAA 1 cut(s) 538
SatI GCNGC 2 cut(s) 222, 494
Sau3AI GATC 2 cut(s) 101, 167
ScrFI CCNGG 3 cut(s) 63, 132, 279
SduI GDGCHC 1 cut(s) 315
SetI ASST 8 cut(s) 154, 177, 277, 310, 352, 370, 469, 498
SfaNI GCATC 1 cut(s) 475
SmiMI CAYNNNNRTG 1 cut(s) 208
SmlI CTYRAG 2 cut(s) 110, 500
SmoI CTYRAG 2 cut(s) 110, 500
Sse9I AATT 2 cut(s) 289, 400
SseBI AGGCCT 1 cut(s) 481
SsiI CCGC 1 cut(s) 38
SspMI CTAG 1 cut(s) 524
StuI AGGCCT 1 cut(s) 481
StyD4I CCNGG 3 cut(s) 61, 130, 277
TaaI ACNGT 1 cut(s) 410
TaqI TCGA 1 cut(s) 187
TasI AATT 2 cut(s) 289, 400
TatI WGTACW 1 cut(s) 510
TfiI GAWTC 1 cut(s) 137
Tru1I TTAA 1 cut(s) 538
Tru9I TTAA 1 cut(s) 538
TscAI CASTG 1 cut(s) 261
TseI GCWGC 2 cut(s) 221, 493
TspDTI ATGAA 1 cut(s) 83
TspRI CASTG 1 cut(s) 261
XceI RCATGY 1 cut(s) 207
XspI CTAG 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.