MD01G1205200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0005000
Physical Location & Seq
Forward (+)
30054202 .. 30054987
786 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1205200.v1.1.491

Sequence Viewer

Length: 786 bp
ATGTCAGGTGGGGTTGGTCCAACCTGCAGTGACATCAGCCTCCCCAAAGAACAAGAGCATGAATTCAAAGAACACAACGACGAAACCTCCTCAAAACTCAGCTCCCACCAAAAGTCCCCAACTTCACACTCCACCGCCACCGCCTCTCCGCGCAAAGCCGGTGGCGGTCTGTTCTCCTTCCGGCAGCTCAACGCCCTCGCCGTCGTCGTCGTCCTCTCCGCCAGCGGCATGGTAAGCCCCCAAGACTTCGCCTTCGTCGTCTTCTCCGTGATCTACATGCACTTCATCTCCAAAGTTGCTTTCCCCATCCTCGGTTCCTCCAGAGACCCCACGGTTTTCAACCCCCAAAACAAAGTCCTCCGCCTCTACGTCCTAACCGGCGCTATCATCGGCCTCCTGCTCCCCATTGCCTACATTTTGGAGGGAATTTTCGAAGGCGATAAACAAGGCATCAGCGCCGCTTCCCCGCACGTTTTTCTCCTTGCCAGTCAGGTGTTCATGGAAGGAATGGCTTTTGCCGACCGGTTTTCGACTCCCATACGTGTTTTCGTGCCGGTTTTCTACAATTCCAGGCGGATTTTCACCATTGTGGAGTGGCTGAAGAGCGAGTTTTCTAAGGAGCATGAGGAGTACGGTGGATCTGCAAAAAGATTGTATGTGGGGAGAGGGCTTGCTATTGCTAATATGGCGTTTTGGTGCTTCAATTTATTTGGGTTCTTGCTGCCTGTCTATCTTCCTAGGGCTTTCAAGAAGTACTACTCCGCCCATAAACTGAAAGATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

262

Amino Acids

28.98

Weight (kDa)

9.24

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7733 PF24867 58 - 253 3.2e-94 Domain of unknown function (DUF7733)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 32
AccII CGCG 1 cut(s) 151
AclWI GGATC 1 cut(s) 646
AcsI RAATTY 2 cut(s) 62, 426
AcuI CTGAAG 1 cut(s) 620
AfaI GTAC 2 cut(s) 632, 755
AfiI CCNNNNNNNGG 1 cut(s) 311
AflIII ACRYGT 1 cut(s) 541
AgeI ACCGGT 1 cut(s) 522
AgsI TTSAA 4 cut(s) 67, 340, 703, 748
AjnI CCWGG 1 cut(s) 569
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
AleI CACNNNNGTG 1 cut(s) 587
AluBI AGCT 2 cut(s) 102, 187
AluI AGCT 2 cut(s) 102, 187
Alw26I GTCTC 1 cut(s) 318
AlwI GGATC 1 cut(s) 646
AoxI GGCC 1 cut(s) 391
ApeKI GCWGC 2 cut(s) 184, 721
ApoI RAATTY 2 cut(s) 62, 426
ArsI GACNNNNNNTTYG 2 cut(s) 236, 268
AsiGI ACCGGT 1 cut(s) 522
AspA2I CCTAGG 1 cut(s) 737
AspLEI GCGC 3 cut(s) 153, 383, 458
AspS9I GGNCC 1 cut(s) 17
AsuHPI GGTGA 1 cut(s) 574
AsuII TTCGAA 1 cut(s) 432
AvaII GGWCC 1 cut(s) 17
AvrII CCTAGG 1 cut(s) 737
BbsI GAAGAC 1 cut(s) 253
BbvI GCAGC 2 cut(s) 196, 708
BccI CCATC 1 cut(s) 314
BceAI ACGGC 1 cut(s) 185
BciT130I CCWGG 1 cut(s) 571
BcoDI GTCTC 1 cut(s) 318
BfaI CTAG 1 cut(s) 738
BfmI CTRYAG 1 cut(s) 25
BfoI RGCGCY 2 cut(s) 384, 459
BfuAI ACCTGC 1 cut(s) 32
BisI GCNGC 4 cut(s) 185, 226, 459, 722
BlnI CCTAGG 1 cut(s) 737
BlsI GCNGC 4 cut(s) 186, 227, 460, 723
BmcAI AGTACT 1 cut(s) 755
Bme1390I CCNGG 1 cut(s) 571
Bme18I GGWCC 1 cut(s) 17
BmgT120I GGNCC 1 cut(s) 17
BmiI GGNNCC 1 cut(s) 316
BmrFI CCNGG 1 cut(s) 571
BmsI GCATC 1 cut(s) 459
BpiI GAAGAC 1 cut(s) 253
BpmI CTGGAG 1 cut(s) 304
Bpu14I TTCGAA 1 cut(s) 432
BsaAI YACGTR 1 cut(s) 542
BsaI GGTCTC 1 cut(s) 318
BsaJI CCNNGG 3 cut(s) 310, 330, 737
BsaWI WCCGGW 1 cut(s) 522
BsaXI ACNNNNNCTCC 6 cut(s) 71, 101, 130, 160, 272, 302
Bsc4I CCNNNNNNNGG 1 cut(s) 311
Bse118I RCCGGY 4 cut(s) 158, 377, 522, 553
Bse1I ACTGG 1 cut(s) 486
Bse3DI GCAATG 1 cut(s) 405
BseBI CCWGG 1 cut(s) 571
BseDI CCNNGG 3 cut(s) 310, 330, 737
BseGI GGATG 1 cut(s) 306
BseLI CCNNNNNNNGG 1 cut(s) 311
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 1 cut(s) 112
BseNI ACTGG 1 cut(s) 486
BseRI GAGGAG 2 cut(s) 79, 641
BseXI GCAGC 2 cut(s) 196, 708
Bsh1236I CGCG 1 cut(s) 151
Bsh1285I CGRYCG 1 cut(s) 523
BshFI GGCC 1 cut(s) 393
BshTI ACCGGT 1 cut(s) 522
BsiEI CGRYCG 1 cut(s) 523
BsiSI CCGG 5 cut(s) 159, 181, 378, 523, 554
BslFI GGGAC 1 cut(s) 100
BslI CCNNNNNNNGG 1 cut(s) 311
BsmAI GTCTC 1 cut(s) 318
BsmFI GGGAC 1 cut(s) 100
BsnI GGCC 1 cut(s) 393
Bso31I GGTCTC 1 cut(s) 318
Bsp119I TTCGAA 1 cut(s) 432
Bsp143I GATC 2 cut(s) 270, 638
BspANI GGCC 1 cut(s) 393
BspCNI CTCAG 1 cut(s) 111
BspFNI CGCG 1 cut(s) 151
BspLI GGNNCC 1 cut(s) 316
BspMAI CTGCAG 1 cut(s) 29
BspMI ACCTGC 1 cut(s) 32
BspPI GGATC 1 cut(s) 646
BspQI GCTCTTC 1 cut(s) 596
BspT104I TTCGAA 1 cut(s) 432
BspTNI GGTCTC 1 cut(s) 318
BsrDI GCAATG 1 cut(s) 405
BsrFI RCCGGY 4 cut(s) 158, 377, 522, 553
BsrI ACTGG 1 cut(s) 486
BssAI RCCGGY 4 cut(s) 158, 377, 522, 553
BssECI CCNNGG 3 cut(s) 310, 330, 737
BssMI GATC 2 cut(s) 270, 638
BssT1I CCWWGG 1 cut(s) 737
Bst2UI CCWGG 1 cut(s) 571
Bst4CI ACNGT 2 cut(s) 334, 635
Bst6I CTCTTC 1 cut(s) 596
BstBAI YACGTR 1 cut(s) 542
BstBI TTCGAA 1 cut(s) 432
BstC8I GCNNGC 2 cut(s) 223, 672
BstDEI CTNAG 2 cut(s) 98, 615
BstDSI CCRYGG 1 cut(s) 330
BstF5I GGATG 1 cut(s) 306
BstFNI CGCG 1 cut(s) 151
BstH2I RGCGCY 2 cut(s) 384, 459
BstHHI GCGC 3 cut(s) 153, 383, 458
BstKTI GATC 2 cut(s) 273, 641
BstMAI GTCTC 1 cut(s) 318
BstMBI GATC 2 cut(s) 270, 638
BstMCI CGRYCG 1 cut(s) 523
BstMWI GCNNNNNNNGC 2 cut(s) 234, 686
BstNI CCWGG 1 cut(s) 571
BstNSI RCATGY 1 cut(s) 280
BstSCI CCNGG 1 cut(s) 569
BstSFI CTRYAG 1 cut(s) 25
BstUI CGCG 1 cut(s) 151
BstV1I GCAGC 2 cut(s) 196, 708
BstV2I GAAGAC 1 cut(s) 253
BstX2I RGATCY 1 cut(s) 638
BstXI CCANNNNNNTGG 1 cut(s) 229
BstYI RGATCY 1 cut(s) 638
BsuRI GGCC 1 cut(s) 393
BtgI CCRYGG 1 cut(s) 330
BtsCI GGATG 1 cut(s) 306
BtsI GCAGTG 1 cut(s) 34
BtsIMutI CAGTG 1 cut(s) 34
BveI ACCTGC 1 cut(s) 32
Cac8I GCNNGC 2 cut(s) 223, 672
CfoI GCGC 3 cut(s) 153, 383, 458
Cfr10I RCCGGY 4 cut(s) 158, 377, 522, 553
Cfr13I GGNCC 1 cut(s) 17
Csp6I GTAC 2 cut(s) 631, 754
CspAI ACCGGT 1 cut(s) 522
CspCI CAANNNNNGTGG 2 cut(s) 142, 177
CviAII CATG 5 cut(s) 59, 229, 277, 499, 623
CviQI GTAC 2 cut(s) 631, 754
DdeI CTNAG 2 cut(s) 98, 615
DpnI GATC 2 cut(s) 272, 640
DpnII GATC 2 cut(s) 270, 638
Eam1104I CTCTTC 1 cut(s) 596
EarI CTCTTC 1 cut(s) 596
EciI GGCGGA 4 cut(s) 208, 350, 589, 751
Eco130I CCWWGG 1 cut(s) 737
Eco31I GGTCTC 1 cut(s) 318
Eco47I GGWCC 1 cut(s) 17
Eco57I CTGAAG 1 cut(s) 620
EcoRI GAATTC 1 cut(s) 62
EcoRII CCWGG 1 cut(s) 569
EcoT14I CCWWGG 1 cut(s) 737
ErhI CCWWGG 1 cut(s) 737
FaeI CATG 5 cut(s) 62, 232, 280, 502, 626
FaiI YATR 9 cut(s) 60, 230, 278, 500, 539, 624, 657, 686, 768
FaqI GGGAC 1 cut(s) 100
FatI CATG 5 cut(s) 58, 228, 276, 498, 622
FauI CCCGC 1 cut(s) 474
Fnu4HI GCNGC 4 cut(s) 185, 226, 459, 722
FokI GGATG 1 cut(s) 293
Fsp4HI GCNGC 4 cut(s) 185, 226, 459, 722
FspBI CTAG 1 cut(s) 738
GlaI GCGC 3 cut(s) 152, 382, 457
GluI GCNGC 4 cut(s) 185, 226, 459, 722
GsuI CTGGAG 1 cut(s) 304
HaeII RGCGCY 2 cut(s) 384, 459
HaeIII GGCC 1 cut(s) 393
HapII CCGG 5 cut(s) 159, 181, 378, 523, 554
HhaI GCGC 3 cut(s) 153, 383, 458
Hin1II CATG 5 cut(s) 62, 232, 280, 502, 626
Hin6I GCGC 3 cut(s) 151, 381, 456
HinP1I GCGC 3 cut(s) 151, 381, 456
HinfI GANTC 1 cut(s) 532
HpaII CCGG 5 cut(s) 159, 181, 378, 523, 554
HphI GGTGA 1 cut(s) 574
Hpy188III TCNNGA 2 cut(s) 321, 748
Hpy99I CGWCG 5 cut(s) 83, 206, 209, 212, 260
HpyAV CCTTC 4 cut(s) 187, 262, 428, 497
HpyCH4III ACNGT 2 cut(s) 334, 635
HpyCH4IV ACGT 3 cut(s) 369, 471, 541
HpyCH4V TGCA 3 cut(s) 27, 280, 644
HpyF10VI GCNNNNNNNGC 2 cut(s) 234, 686
HpyF3I CTNAG 2 cut(s) 98, 615
HpySE526I ACGT 3 cut(s) 369, 471, 541
Hsp92II CATG 5 cut(s) 62, 232, 280, 502, 626
HspAI GCGC 3 cut(s) 151, 381, 456
Kzo9I GATC 2 cut(s) 270, 638
LguI GCTCTTC 1 cut(s) 596
LmnI GCTCC 3 cut(s) 107, 405, 619
Lsp1109I GCAGC 2 cut(s) 196, 708
LweI GCATC 1 cut(s) 459
MaeI CTAG 1 cut(s) 738
MaeII ACGT 3 cut(s) 369, 471, 541
MaeIII GTNAC 1 cut(s) 29
MalI GATC 2 cut(s) 272, 640
MboI GATC 2 cut(s) 270, 638
MboII GAAGA 3 cut(s) 253, 613, 725
MflI RGATCY 1 cut(s) 638
MluCI AATT 4 cut(s) 62, 426, 565, 703
MlyI GAGTC 1 cut(s) 526
MmeI TCCRAC 1 cut(s) 44
MslI CAYNNNNRTG 1 cut(s) 587
MspA1I CMGCKG 1 cut(s) 225
MspI CCGG 5 cut(s) 159, 181, 378, 523, 554
MspR9I CCNGG 1 cut(s) 571
MvaI CCWGG 1 cut(s) 571
MvnI CGCG 1 cut(s) 151
MwoI GCNNNNNNNGC 2 cut(s) 234, 686
NdeII GATC 2 cut(s) 270, 638
NlaIII CATG 5 cut(s) 62, 232, 280, 502, 626
NlaIV GGNNCC 1 cut(s) 316
NmuCI GTSAC 1 cut(s) 29
NspI RCATGY 1 cut(s) 280
NspV TTCGAA 1 cut(s) 432
OliI CACNNNNGTG 1 cut(s) 587
PciSI GCTCTTC 1 cut(s) 596
PcsI WCGNNNNNNNCGW 4 cut(s) 198, 204, 255, 264
PinAI ACCGGT 1 cut(s) 522
PkrI GCNGC 4 cut(s) 186, 227, 460, 723
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
Ppu21I YACGTR 1 cut(s) 542
Psp6I CCWGG 1 cut(s) 569
PspGI CCWGG 1 cut(s) 569
PspN4I GGNNCC 1 cut(s) 316
PspPI GGNCC 1 cut(s) 17
PstI CTGCAG 1 cut(s) 29
PsuI RGATCY 1 cut(s) 638
RsaI GTAC 2 cut(s) 632, 755
RsaNI GTAC 2 cut(s) 631, 754
RseI CAYNNNNRTG 1 cut(s) 587
SapI GCTCTTC 1 cut(s) 596
SatI GCNGC 4 cut(s) 185, 226, 459, 722
Sau3AI GATC 2 cut(s) 270, 638
Sau96I GGNCC 1 cut(s) 17
ScaI AGTACT 1 cut(s) 755
SchI GAGTC 1 cut(s) 526
ScrFI CCNGG 1 cut(s) 571
SetI ASST 9 cut(s) 10, 26, 89, 104, 189, 372, 474, 495, 544
SfaNI GCATC 1 cut(s) 459
SfcI CTRYAG 1 cut(s) 25
SfuI TTCGAA 1 cut(s) 432
SinI GGWCC 1 cut(s) 17
SmiMI CAYNNNNRTG 1 cut(s) 587
Sse9I AATT 4 cut(s) 62, 426, 565, 703
SspMI CTAG 1 cut(s) 738
StyD4I CCNGG 1 cut(s) 569
StyI CCWWGG 1 cut(s) 737
TaaI ACNGT 2 cut(s) 334, 635
TaiI ACGT 3 cut(s) 372, 474, 544
TaqI TCGA 2 cut(s) 432, 530
TasI AATT 4 cut(s) 62, 426, 565, 703
TatI WGTACW 1 cut(s) 753
TauI GCSGC 2 cut(s) 228, 461
TscAI CASTG 1 cut(s) 34
TseFI GTSAC 1 cut(s) 29
TseI GCWGC 2 cut(s) 184, 721
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 3 cut(s) 75, 274, 487
TspGWI ACGGA 1 cut(s) 256
TspRI CASTG 1 cut(s) 34
VpaK11BI GGWCC 1 cut(s) 17
XapI RAATTY 2 cut(s) 62, 426
XceI RCATGY 1 cut(s) 280
XmaJI CCTAGG 1 cut(s) 737
XspI CTAG 1 cut(s) 738
ZrmI AGTACT 1 cut(s) 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.