MD07G1273700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005000
Physical Location & Seq
Forward (+)
33941501 .. 33942061
561 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1273700.v1.1.491

Sequence Viewer

Length: 561 bp
ATGGTCAGCCCCGGGGACATGGCTTTCGTTCTCTTCTCCTTCCTCTACATCTTCTTCCTCTCAAAGTTTGTGTTCCCCATCCCCCCTTCTTCTCAAGAGCCCCAATTCTTCAACCCCCGCAACAAGCTCCTTCGCCTCTACATTGTCCTCAGCGTCCTCATCGGCCTTGTCCTCCCCATAGCCTACATCTTCGAGGGCGTTCTCGAGAACGACCAGCTAGGGATCAAAGCAGCTACACCACCTGTTTTTCTCTTTGCGTGTCAACTTTTCATGGACGGAGTGGTTTTTGGTGACAGATTTTCCACTCCGATACGCATTTTCGTGCCGGTTTTGTACAACTCCAAGAGGATTTTTACCCTAGTGGATTGGCTTAGAAGTGAGTTCTCGAAACAAGATGAAGAAGTGTACGGTGGCTCTGCGAGGAGGCTGTACGTTGGGAGAGTGCTTGCAGTTGTTAACTTGGCGTTTTGGTCGTTTAATCTTTTCGGGTGCTTGTTGCCTGTTTATCTTCCTAAGGCTTTCAAGAAACATTACTCGGCTTACAGTGAAGTCAAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

187

Amino Acids

21.32

Weight (kDa)

9.08

Isoelectric Point (pI)

46.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7733 PF24867 1 - 178 2.6e-76 Domain of unknown function (DUF7733)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 118
AclWI GGATC 1 cut(s) 230
AfaI GTAC 3 cut(s) 335, 407, 431
AgsI TTSAA 2 cut(s) 112, 523
AluBI AGCT 3 cut(s) 127, 217, 233
AluI AGCT 3 cut(s) 127, 217, 233
AlwI GGATC 1 cut(s) 230
Ama87I CYCGRG 2 cut(s) 11, 203
AoxI GGCC 1 cut(s) 163
ApeKI GCWGC 1 cut(s) 230
ArsI GACNNNNNNTTYG 2 cut(s) 8, 40
AsuC2I CCSGG 2 cut(s) 12, 13
AsuHPI GGTGA 1 cut(s) 302
AvaI CYCGRG 2 cut(s) 11, 203
AxyI CCTNAGG 1 cut(s) 513
BanII GRGCYC 1 cut(s) 102
BbvCI CCTCAGC 1 cut(s) 149
BbvI GCAGC 1 cut(s) 242
BccI CCATC 1 cut(s) 86
BcnI CCSGG 2 cut(s) 12, 13
BfaI CTAG 2 cut(s) 218, 359
BisI GCNGC 1 cut(s) 231
BlsI GCNGC 1 cut(s) 232
Bme1390I CCNGG 2 cut(s) 12, 13
BmeT110I CYCGRG 2 cut(s) 11, 203
BmrFI CCNGG 2 cut(s) 12, 13
Bpu10I CCTNAGC 1 cut(s) 149
BpuEI CTTGAG 1 cut(s) 78
BpuMI CCSGG 2 cut(s) 12, 13
BsaJI CCNNGG 3 cut(s) 10, 11, 12
Bse118I RCCGGY 1 cut(s) 325
Bse21I CCTNAGG 1 cut(s) 513
BseDI CCNNGG 3 cut(s) 10, 11, 12
BseGI GGATG 1 cut(s) 78
BseMII CTCAG 1 cut(s) 163
BseRI GAGGAG 1 cut(s) 436
BseXI GCAGC 1 cut(s) 242
BshFI GGCC 1 cut(s) 165
BsiHKCI CYCGRG 2 cut(s) 11, 203
BsiSI CCGG 2 cut(s) 12, 326
BslFI GGGAC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 29
BsnI GGCC 1 cut(s) 165
BsoBI CYCGRG 2 cut(s) 11, 203
Bsp1286I GDGCHC 1 cut(s) 102
Bsp1407I TGTACA 1 cut(s) 333
Bsp143I GATC 1 cut(s) 222
BspACI CCGC 1 cut(s) 118
BspANI GGCC 1 cut(s) 165
BspCNI CTCAG 1 cut(s) 162
BspPI GGATC 1 cut(s) 230
BsrFI RCCGGY 1 cut(s) 325
BsrGI TGTACA 1 cut(s) 333
BssAI RCCGGY 1 cut(s) 325
BssECI CCNNGG 3 cut(s) 10, 11, 12
BssMI GATC 1 cut(s) 222
Bst4CI ACNGT 2 cut(s) 410, 545
Bst6I CTCTTC 1 cut(s) 38
BstAUI TGTACA 1 cut(s) 333
BstC8I GCNNGC 1 cut(s) 447
BstDEI CTNAG 3 cut(s) 149, 371, 513
BstF5I GGATG 1 cut(s) 78
BstKTI GATC 1 cut(s) 225
BstMBI GATC 1 cut(s) 222
BstSCI CCNGG 2 cut(s) 10, 11
BstV1I GCAGC 1 cut(s) 242
Bsu36I CCTNAGG 1 cut(s) 513
BsuRI GGCC 1 cut(s) 165
BtsCI GGATG 1 cut(s) 78
BtsIMutI CAGTG 1 cut(s) 550
Cac8I GCNNGC 1 cut(s) 447
Cfr10I RCCGGY 1 cut(s) 325
Cfr9I CCCGGG 1 cut(s) 11
CseI GACGC 1 cut(s) 142
Csp6I GTAC 3 cut(s) 334, 406, 430
CviAII CATG 2 cut(s) 19, 271
CviQI GTAC 3 cut(s) 334, 406, 430
DdeI CTNAG 3 cut(s) 149, 371, 513
DpnI GATC 1 cut(s) 224
DpnII GATC 1 cut(s) 222
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Eco24I GRGCYC 1 cut(s) 102
Eco81I CCTNAGG 1 cut(s) 513
Eco88I CYCGRG 2 cut(s) 11, 203
EcoT38I GRGCYC 1 cut(s) 102
FaeI CATG 2 cut(s) 22, 274
FaiI YATR 3 cut(s) 20, 179, 272
FaqI GGGAC 1 cut(s) 29
FatI CATG 2 cut(s) 18, 270
FauI CCCGC 1 cut(s) 125
Fnu4HI GCNGC 1 cut(s) 231
FokI GGATG 1 cut(s) 65
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 1 cut(s) 231
FspBI CTAG 2 cut(s) 218, 359
GluI GCNGC 1 cut(s) 231
HaeIII GGCC 1 cut(s) 165
HapII CCGG 2 cut(s) 12, 326
HgaI GACGC 1 cut(s) 142
Hin1II CATG 2 cut(s) 22, 274
HincII GTYRAC 2 cut(s) 263, 457
HindII GTYRAC 2 cut(s) 263, 457
HpaI GTTAAC 1 cut(s) 457
HpaII CCGG 2 cut(s) 12, 326
HphI GGTGA 1 cut(s) 302
Hpy166II GTNNAC 3 cut(s) 263, 406, 457
Hpy188I TCNGA 1 cut(s) 309
Hpy188III TCNNGA 5 cut(s) 95, 203, 205, 385, 523
Hpy8I GTNNAC 3 cut(s) 263, 406, 457
HpyAV CCTTC 3 cut(s) 49, 96, 140
HpyCH4III ACNGT 2 cut(s) 410, 545
HpyCH4IV ACGT 1 cut(s) 432
HpyCH4V TGCA 1 cut(s) 449
HpyF3I CTNAG 3 cut(s) 149, 371, 513
HpySE526I ACGT 1 cut(s) 432
Hsp92II CATG 2 cut(s) 22, 274
KspAI GTTAAC 1 cut(s) 457
Kzo9I GATC 1 cut(s) 222
LmnI GCTCC 1 cut(s) 132
LpnPI CCDG 5 cut(s) 25, 227, 255, 339, 513
Lsp1109I GCAGC 1 cut(s) 242
MaeI CTAG 2 cut(s) 218, 359
MaeII ACGT 1 cut(s) 432
MaeIII GTNAC 1 cut(s) 290
MalI GATC 1 cut(s) 224
MboI GATC 1 cut(s) 222
MboII GAAGA 8 cut(s) 25, 43, 46, 81, 100, 181, 410, 500
MhlI GDGCHC 1 cut(s) 102
MluCI AATT 1 cut(s) 104
MseI TTAA 2 cut(s) 456, 477
MslI CAYNNNNRTG 1 cut(s) 320
MspI CCGG 2 cut(s) 12, 326
MspR9I CCNGG 2 cut(s) 12, 13
NciI CCSGG 2 cut(s) 12, 13
NdeII GATC 1 cut(s) 222
NlaIII CATG 2 cut(s) 22, 274
NmeAIII GCCGAG 1 cut(s) 515
NmuCI GTSAC 1 cut(s) 290
PaeR7I CTCGAG 1 cut(s) 203
PkrI GCNGC 1 cut(s) 232
RsaI GTAC 3 cut(s) 335, 407, 431
RsaNI GTAC 3 cut(s) 334, 406, 430
RseI CAYNNNNRTG 1 cut(s) 320
SaqAI TTAA 2 cut(s) 456, 477
SatI GCNGC 1 cut(s) 231
Sau3AI GATC 1 cut(s) 222
ScrFI CCNGG 2 cut(s) 12, 13
SduI GDGCHC 1 cut(s) 102
SetI ASST 5 cut(s) 129, 219, 235, 244, 435
Sfr274I CTCGAG 1 cut(s) 203
SlaI CTCGAG 1 cut(s) 203
SmaI CCCGGG 1 cut(s) 13
SmiMI CAYNNNNRTG 1 cut(s) 320
SmlI CTYRAG 2 cut(s) 93, 203
SmoI CTYRAG 2 cut(s) 93, 203
Sse9I AATT 1 cut(s) 104
SsiI CCGC 1 cut(s) 118
SspMI CTAG 2 cut(s) 218, 359
StyD4I CCNGG 2 cut(s) 10, 11
TaaI ACNGT 2 cut(s) 410, 545
TaiI ACGT 1 cut(s) 435
TaqI TCGA 3 cut(s) 192, 204, 386
TasI AATT 1 cut(s) 104
TatI WGTACW 1 cut(s) 333
Tru1I TTAA 2 cut(s) 456, 477
Tru9I TTAA 2 cut(s) 456, 477
TscAI CASTG 1 cut(s) 550
TseFI GTSAC 1 cut(s) 290
TseI GCWGC 1 cut(s) 230
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 2 cut(s) 259, 411
TspGWI ACGGA 1 cut(s) 291
TspMI CCCGGG 1 cut(s) 11
TspRI CASTG 1 cut(s) 550
XhoI CTCGAG 1 cut(s) 203
XmaI CCCGGG 1 cut(s) 11
XspI CTAG 2 cut(s) 218, 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.