pycom07g25030

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005000
Physical Location & Seq
Forward (+)
25476962 .. 25477975
1014 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g25030.1

Sequence Viewer

Length: 1014 bp
ATGATGTCCACTCCCTCTCCTCTCAGCAAAACCAGCTTCCTGAAACCCCTATTACACTGCCACCTGTCCACCCTCATCACCTTATGCTTTACCCTAACGTTTCCTTCTCCCCAGCTCTTCCTCTTAATTCTCTCTCTAACCTTCTTTCACTCTCTCACAATTTTCTCTCTCTTCAACTCTTCTTTCTCTGCAAGAAACCCTAACACTAATCAACAAGAACACAAAAATATGTCAGGTGGAGTTGGTCCAACCTGCAGTGACATTAGTCTCCCCAAAGAACAAGAACATGAATTCAAGGAACACAACGACCAAACCTCCTCAAAACTCACCTCCCACCAGACGTTCCCATCTTTACACTCCGGCGCCACCGCCTCTCCGCTCAAAGCCGGTGGCGGTACGTTCTCCTTCCGGCAACTCAACGCCCTCGCCGTCGTCGTCGTCTTCTCCGCCAGCGGCATGGTAAGTCCCCAAGACTTTGCCTTCGTTGTCTTCTCCATGATCTACATGAACTTCATCTCCAAAGTTGCTTTCCCCGCCCTCAGTTCCTCTAAAGACCCCACAGTTTTCAACCCAAAAAACAAAATCCTCCGCCTCTACGTCCTAACGGGGGCCATCATCGGCCTTCTGCTCCCCATTGCCTACATTTTTGAGGGCATTTTCGAAGGCGATAAAGAAGGCATCAGCGCCGCTTCCCCGCACGTTTTTCTCCTCGCAAGTCAGGTGTTCATGGAAGGAATGGCTTTCGCCGACCGGTTCTCGACGCCCATACGTGTTTTCGTGCCGGTTTTCTACAATTCCAGGAGGATATTCACCATTGTGGAGTGGCTGAAGAGTGAGTTTTCCAAAGAATACGAGGAGTATGGTGGATCTGCAAGAAGATTGTACGTTGGGAGAGGACTTGCTATTGCTAACATGGGGTTTTGGTGCTTCAATTTATTTGGGTTCTTGTTGCCTATCTATCTTCCTAGGGCTTTCAAGAAGTATTACTCTGCCCATAAACTGAAAGACTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

338

Amino Acids

37.66

Weight (kDa)

9.25

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7733 PF24867 134 - 329 1.2e-93 Domain of unknown function (DUF7733)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 260
AccB1I GGYRCC 1 cut(s) 362
AccBSI CCGCTC 1 cut(s) 379
AciI CCGC 9 cut(s) 369, 377, 393, 447, 453, 534, 589, 687, 695
AclI AACGTT 1 cut(s) 98
AclWI GGATC 1 cut(s) 874
AcsI RAATTY 1 cut(s) 290
AcuI CTGAAG 1 cut(s) 848
AcyI GRCGYC 2 cut(s) 363, 761
AfaI GTAC 2 cut(s) 397, 884
AfiI CCNNNNNNNGG 1 cut(s) 607
AflIII ACRYGT 1 cut(s) 769
AgeI ACCGGT 1 cut(s) 750
AgsI TTSAA 5 cut(s) 175, 295, 568, 931, 976
AjnI CCWGG 1 cut(s) 797
AjuI GAANNNNNNNTTGG 2 cut(s) 512, 544
AleI CACNNNNGTG 1 cut(s) 815
AloI GAACNNNNNNTCC 2 cut(s) 500, 532
AluBI AGCT 2 cut(s) 36, 115
AluI AGCT 2 cut(s) 36, 115
Alw26I GTCTC 1 cut(s) 272
AlwI GGATC 1 cut(s) 874
AoxI GGCC 2 cut(s) 609, 619
ApoI RAATTY 1 cut(s) 290
ArsI GACNNNNNNTTYG 2 cut(s) 464, 496
AsiGI ACCGGT 1 cut(s) 750
AspA2I CCTAGG 1 cut(s) 965
AspLEI GCGC 2 cut(s) 365, 686
AspS9I GGNCC 2 cut(s) 245, 609
AsuHPI GGTGA 3 cut(s) 70, 319, 802
AsuII TTCGAA 1 cut(s) 660
AvaII GGWCC 1 cut(s) 245
AvrII CCTAGG 1 cut(s) 965
BanI GGYRCC 1 cut(s) 362
BbsI GAAGAC 2 cut(s) 433, 481
BccI CCATC 2 cut(s) 355, 620
BceAI ACGGC 1 cut(s) 413
BciT130I CCWGG 1 cut(s) 799
BcoDI GTCTC 1 cut(s) 272
BfaI CTAG 1 cut(s) 966
BfmI CTRYAG 1 cut(s) 253
BfoI RGCGCY 2 cut(s) 366, 687
BfuAI ACCTGC 1 cut(s) 260
BisI GCNGC 2 cut(s) 454, 687
BlnI CCTAGG 1 cut(s) 965
BlsI GCNGC 2 cut(s) 455, 688
Bme1390I CCNGG 1 cut(s) 799
Bme18I GGWCC 1 cut(s) 245
BmgT120I GGNCC 2 cut(s) 245, 609
BmiI GGNNCC 2 cut(s) 364, 610
BmrFI CCNGG 1 cut(s) 799
BmsI GCATC 1 cut(s) 687
BoxI GACNNNNGTC 1 cut(s) 264
BpiI GAAGAC 2 cut(s) 433, 481
Bpu14I TTCGAA 1 cut(s) 660
BsaAI YACGTR 1 cut(s) 770
BsaHI GRCGYC 2 cut(s) 363, 761
BsaJI CCNNGG 1 cut(s) 965
BsaWI WCCGGW 1 cut(s) 750
BsaXI ACNNNNNCTCC 7 cut(s) 31, 299, 329, 358, 388, 500, 530
Bsc4I CCNNNNNNNGG 1 cut(s) 607
Bse118I RCCGGY 3 cut(s) 386, 750, 781
Bse3DI GCAATG 1 cut(s) 633
BseBI CCWGG 1 cut(s) 799
BseDI CCNNGG 1 cut(s) 965
BseLI CCNNNNNNNGG 1 cut(s) 607
BseMI GCAATG 1 cut(s) 633
BseMII CTCAG 2 cut(s) 37, 553
BseRI GAGGAG 4 cut(s) 9, 307, 698, 869
BseYI CCCAGC 1 cut(s) 111
Bsh1285I CGRYCG 1 cut(s) 751
BshFI GGCC 2 cut(s) 611, 621
BshNI GGYRCC 1 cut(s) 362
BshTI ACCGGT 1 cut(s) 750
BsiEI CGRYCG 1 cut(s) 751
BsiSI CCGG 5 cut(s) 360, 387, 409, 751, 782
BslFI GGGAC 1 cut(s) 450
BslI CCNNNNNNNGG 1 cut(s) 607
BsmAI GTCTC 1 cut(s) 272
BsmFI GGGAC 1 cut(s) 450
BsnI GGCC 2 cut(s) 611, 621
Bsp119I TTCGAA 1 cut(s) 660
Bsp143I GATC 2 cut(s) 498, 866
BspACI CCGC 9 cut(s) 369, 377, 393, 447, 453, 534, 589, 687, 695
BspANI GGCC 2 cut(s) 611, 621
BspCNI CTCAG 2 cut(s) 36, 552
BspLI GGNNCC 2 cut(s) 364, 610
BspMAI CTGCAG 1 cut(s) 257
BspMI ACCTGC 1 cut(s) 260
BspPI GGATC 1 cut(s) 874
BspQI GCTCTTC 1 cut(s) 122
BspT104I TTCGAA 1 cut(s) 660
BspT107I GGYRCC 1 cut(s) 362
BsrBI CCGCTC 1 cut(s) 379
BsrDI GCAATG 1 cut(s) 633
BsrFI RCCGGY 3 cut(s) 386, 750, 781
BssAI RCCGGY 3 cut(s) 386, 750, 781
BssECI CCNNGG 1 cut(s) 965
BssMI GATC 2 cut(s) 498, 866
BssNI GRCGYC 2 cut(s) 363, 761
BssT1I CCWWGG 1 cut(s) 965
Bst2UI CCWGG 1 cut(s) 799
Bst4CI ACNGT 1 cut(s) 562
Bst6I CTCTTC 4 cut(s) 122, 176, 184, 824
BstACI GRCGYC 2 cut(s) 363, 761
BstBAI YACGTR 1 cut(s) 770
BstBI TTCGAA 1 cut(s) 660
BstC8I GCNNGC 1 cut(s) 451
BstDEI CTNAG 2 cut(s) 23, 539
BstH2I RGCGCY 2 cut(s) 366, 687
BstHHI GCGC 2 cut(s) 365, 686
BstKTI GATC 2 cut(s) 501, 869
BstMAI GTCTC 1 cut(s) 272
BstMBI GATC 2 cut(s) 498, 866
BstMCI CGRYCG 1 cut(s) 751
BstMWI GCNNNNNNNGC 2 cut(s) 33, 533
BstNI CCWGG 1 cut(s) 799
BstPAI GACNNNNGTC 1 cut(s) 264
BstSCI CCNGG 1 cut(s) 797
BstSFI CTRYAG 1 cut(s) 253
BstV2I GAAGAC 2 cut(s) 433, 481
BstX2I RGATCY 1 cut(s) 866
BstXI CCANNNNNNTGG 1 cut(s) 457
BstYI RGATCY 1 cut(s) 866
BsuRI GGCC 2 cut(s) 611, 621
BtsI GCAGTG 2 cut(s) 55, 262
BtsIMutI CAGTG 2 cut(s) 55, 262
BveI ACCTGC 1 cut(s) 260
Cac8I GCNNGC 1 cut(s) 451
CfoI GCGC 2 cut(s) 365, 686
Cfr10I RCCGGY 3 cut(s) 386, 750, 781
Cfr13I GGNCC 2 cut(s) 245, 609
CseI GACGC 1 cut(s) 769
Csp6I GTAC 2 cut(s) 396, 883
CspAI ACCGGT 1 cut(s) 750
CspCI CAANNNNNGTGG 2 cut(s) 370, 405
CviAII CATG 6 cut(s) 287, 457, 496, 505, 727, 913
CviJI RGCY 8 cut(s) 36, 115, 386, 611, 621, 740, 826, 971
CviKI_1 RGCY 8 cut(s) 36, 115, 386, 611, 621, 740, 826, 971
CviQI GTAC 2 cut(s) 396, 883
DdeI CTNAG 2 cut(s) 23, 539
DinI GGCGCC 1 cut(s) 364
DpnI GATC 2 cut(s) 500, 868
DpnII GATC 2 cut(s) 498, 866
Eam1104I CTCTTC 4 cut(s) 122, 176, 184, 824
EarI CTCTTC 4 cut(s) 122, 176, 184, 824
EciI GGCGGA 2 cut(s) 436, 578
Eco130I CCWWGG 1 cut(s) 965
Eco47I GGWCC 1 cut(s) 245
Eco57I CTGAAG 1 cut(s) 848
EcoRI GAATTC 1 cut(s) 290
EcoRII CCWGG 1 cut(s) 797
EcoT14I CCWWGG 1 cut(s) 965
EgeI GGCGCC 1 cut(s) 364
EheI GGCGCC 1 cut(s) 364
ErhI CCWWGG 1 cut(s) 965
FaeI CATG 6 cut(s) 290, 460, 499, 508, 730, 916
FaqI GGGAC 1 cut(s) 450
FatI CATG 6 cut(s) 286, 456, 495, 504, 726, 912
FauI CCCGC 2 cut(s) 541, 702
Fnu4HI GCNGC 2 cut(s) 454, 687
Fsp4HI GCNGC 2 cut(s) 454, 687
FspBI CTAG 1 cut(s) 966
GlaI GCGC 2 cut(s) 364, 685
GluI GCNGC 2 cut(s) 454, 687
GsaI CCCAGC 1 cut(s) 115
HaeII RGCGCY 2 cut(s) 366, 687
HaeIII GGCC 2 cut(s) 611, 621
HapII CCGG 5 cut(s) 360, 387, 409, 751, 782
HgaI GACGC 1 cut(s) 769
HhaI GCGC 2 cut(s) 365, 686
Hin1I GRCGYC 2 cut(s) 363, 761
Hin1II CATG 6 cut(s) 290, 460, 499, 508, 730, 916
Hin6I GCGC 2 cut(s) 363, 684
HinP1I GCGC 2 cut(s) 363, 684
HpaII CCGG 5 cut(s) 360, 387, 409, 751, 782
HphI GGTGA 3 cut(s) 70, 319, 802
Hpy166II GTNNAC 2 cut(s) 9, 69
Hpy188III TCNNGA 3 cut(s) 40, 757, 976
Hpy8I GTNNAC 2 cut(s) 9, 69
Hpy99I CGWCG 4 cut(s) 434, 437, 440, 763
HpyAV CCTTC 8 cut(s) 114, 151, 415, 490, 632, 656, 668, 725
HpyCH4III ACNGT 1 cut(s) 562
HpyCH4IV ACGT 7 cut(s) 98, 341, 398, 597, 699, 769, 885
HpyCH4V TGCA 3 cut(s) 191, 255, 872
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 533
HpyF3I CTNAG 2 cut(s) 23, 539
HpySE526I ACGT 7 cut(s) 98, 341, 398, 597, 699, 769, 885
Hsp92I GRCGYC 2 cut(s) 363, 761
Hsp92II CATG 6 cut(s) 290, 460, 499, 508, 730, 916
HspAI GCGC 2 cut(s) 363, 684
KasI GGCGCC 1 cut(s) 362
Kzo9I GATC 2 cut(s) 498, 866
LguI GCTCTTC 1 cut(s) 122
LmnI GCTCC 1 cut(s) 633
LweI GCATC 1 cut(s) 687
MaeI CTAG 1 cut(s) 966
MaeII ACGT 7 cut(s) 98, 341, 398, 597, 699, 769, 885
MaeIII GTNAC 1 cut(s) 257
MalI GATC 2 cut(s) 500, 868
MbiI CCGCTC 1 cut(s) 379
MboI GATC 2 cut(s) 498, 866
MboII GAAGA 8 cut(s) 109, 163, 171, 433, 481, 841, 888, 953
MflI RGATCY 1 cut(s) 866
MluCI AATT 5 cut(s) 126, 159, 290, 793, 931
Mly113I GGCGCC 1 cut(s) 363
MmeI TCCRAC 1 cut(s) 272
MseI TTAA 1 cut(s) 125
MslI CAYNNNNRTG 1 cut(s) 815
MspA1I CMGCKG 1 cut(s) 453
MspI CCGG 5 cut(s) 360, 387, 409, 751, 782
MspR9I CCNGG 1 cut(s) 799
MvaI CCWGG 1 cut(s) 799
MwoI GCNNNNNNNGC 2 cut(s) 33, 533
NarI GGCGCC 1 cut(s) 363
NdeII GATC 2 cut(s) 498, 866
NlaIII CATG 6 cut(s) 290, 460, 499, 508, 730, 916
NlaIV GGNNCC 2 cut(s) 364, 610
NmuCI GTSAC 1 cut(s) 257
NspV TTCGAA 1 cut(s) 660
OliI CACNNNNGTG 1 cut(s) 815
PciSI GCTCTTC 1 cut(s) 122
PcsI WCGNNNNNNNCGW 2 cut(s) 426, 432
PfoI TCCNGGA 1 cut(s) 797
PinAI ACCGGT 1 cut(s) 750
PkrI GCNGC 2 cut(s) 455, 688
PluTI GGCGCC 1 cut(s) 366
Ppu21I YACGTR 1 cut(s) 770
PshAI GACNNNNGTC 1 cut(s) 264
Psp1406I AACGTT 1 cut(s) 98
Psp6I CCWGG 1 cut(s) 797
PspFI CCCAGC 1 cut(s) 111
PspGI CCWGG 1 cut(s) 797
PspN4I GGNNCC 2 cut(s) 364, 610
PspPI GGNCC 2 cut(s) 245, 609
PstI CTGCAG 1 cut(s) 257
PsuI RGATCY 1 cut(s) 866
RsaI GTAC 2 cut(s) 397, 884
RsaNI GTAC 2 cut(s) 396, 883
RseI CAYNNNNRTG 1 cut(s) 815
SapI GCTCTTC 1 cut(s) 122
SaqAI TTAA 1 cut(s) 125
SatI GCNGC 2 cut(s) 454, 687
Sau3AI GATC 2 cut(s) 498, 866
Sau96I GGNCC 2 cut(s) 245, 609
ScrFI CCNGG 1 cut(s) 799
SfaNI GCATC 1 cut(s) 687
SfcI CTRYAG 1 cut(s) 253
SfoI GGCGCC 1 cut(s) 364
SfuI TTCGAA 1 cut(s) 660
SinI GGWCC 1 cut(s) 245
SmiMI CAYNNNNRTG 1 cut(s) 815
Sse9I AATT 5 cut(s) 126, 159, 290, 793, 931
SsiI CCGC 9 cut(s) 369, 377, 393, 447, 453, 534, 589, 687, 695
SspDI GGCGCC 1 cut(s) 362
SspMI CTAG 1 cut(s) 966
StyD4I CCNGG 1 cut(s) 797
StyI CCWWGG 1 cut(s) 965
TaaI ACNGT 1 cut(s) 562
TaiI ACGT 7 cut(s) 101, 344, 401, 600, 702, 772, 888
TaqI TCGA 2 cut(s) 660, 758
TasI AATT 5 cut(s) 126, 159, 290, 793, 931
TauI GCSGC 2 cut(s) 456, 689
Tru1I TTAA 1 cut(s) 125
Tru9I TTAA 1 cut(s) 125
TscAI CASTG 2 cut(s) 62, 262
TseFI GTSAC 1 cut(s) 257
Tsp45I GTSAC 1 cut(s) 257
TspDTI ATGAA 4 cut(s) 303, 502, 521, 715
TspRI CASTG 2 cut(s) 62, 262
VpaK11BI GGWCC 1 cut(s) 245
XapI RAATTY 1 cut(s) 290
XmaJI CCTAGG 1 cut(s) 965
XspI CTAG 1 cut(s) 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.