Rh1DG413400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0005000
Physical Location & Seq
Forward (+)
63012461 .. 63013228
768 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG413400.1

Sequence Viewer

Length: 768 bp
ATGTCGTCCGGTGATGAAGCTGGTGACGTCAACGTGCCGGAAAAAGAGTACGATGAGCTCAAACAAAGCGAGGATCCGGTTACTTCAACCTTCAAGCTCGGCCCCCAAAAGGGCCAAAACCTCAAAACTGTCCACAAATATAATTACATACCCTTTTGGCAGCTCAACTGCCTCCTGGTCATGGTAGTCCTCTCGGCCAGTGGCTTGGTAGGCCCTGAAGACCTGGCTTTTGTTCTCTTCTCAATAATTTACCTCTTCTTCCTCTCGAAATTCGCTTTCCCAAAGCTCACTGATCCTTCGGAAGAGCCCCCGGTGTTCGACCCCGAAAGCTATCTAGTTCGGCTCGGCCCTCTCTTTGGTGGCTTCATAGGCATTGTGCTCCCCGTTGCGTACATTTTTGAAGGGTTTATAGAGGGAGACAAAGAAGGAATCAGGGCGGCAGCGCCGCATGTTTTCCTTCTTGCGAGCCAAGTCTTCATGGATGGAGTGGCTTTCAGTGACAGGTTTTCGACGCCAATTAGGGTTTTTGTGCCCGTTTTCTATAATGCCAAGAGGATTTTTACCCTTGTGGATTGGGTTAAGAGAGAGTTTTCGAAAGCTGATGGTCAAGAGTATGGTGGATCTGCAAGTGAGAGGAGACTCTTTCTTGGGAGAGTACTCGCTATAGTTAACTTGGTGTTTTGGTCCTACGATCTCTTCTTCTTCTTGTTGCCTGTTTATCTTCCTAGAGCTTTCAGGAAATATTATGCTGCAGCAAAAGCAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

255

Amino Acids

28.8

Weight (kDa)

5.36

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7733 PF24867 52 - 249 4.6e-83 Domain of unknown function (DUF7733)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 30
AciI CCGC 2 cut(s) 437, 446
AclWI GGATC 4 cut(s) 68, 81, 287, 628
AcoI YGGCCR 1 cut(s) 195
AcsI RAATTY 1 cut(s) 269
AcuI CTGAAG 1 cut(s) 237
AcyI GRCGYC 2 cut(s) 27, 512
AfaI GTAC 3 cut(s) 50, 392, 657
AfiI CCNNNNNNNGG 4 cut(s) 109, 110, 181, 356
AgsI TTSAA 3 cut(s) 87, 94, 401
AjnI CCWGG 2 cut(s) 174, 222
AluBI AGCT 8 cut(s) 20, 58, 97, 163, 286, 330, 599, 731
AluI AGCT 8 cut(s) 20, 58, 97, 163, 286, 330, 599, 731
Alw21I GWGCWC 2 cut(s) 60, 381
Alw26I GTCTC 2 cut(s) 411, 631
AlwI GGATC 4 cut(s) 68, 81, 287, 628
AoxI GGCC 5 cut(s) 100, 112, 195, 211, 346
ApeKI GCWGC 4 cut(s) 160, 440, 749, 752
ApoI RAATTY 1 cut(s) 269
ArsI GACNNNNNNTTYG 2 cut(s) 212, 244
AspLEI GCGC 1 cut(s) 445
AspS9I GGNCC 5 cut(s) 101, 112, 212, 347, 684
AsuC2I CCSGG 1 cut(s) 311
AsuHPI GGTGA 2 cut(s) 23, 35
AsuII TTCGAA 1 cut(s) 593
AvaII GGWCC 1 cut(s) 684
BaeGI GKGCMC 1 cut(s) 534
BamHI GGATCC 1 cut(s) 73
BanII GRGCYC 2 cut(s) 60, 309
BbsI GAAGAC 2 cut(s) 225, 466
Bbv12I GWGCWC 2 cut(s) 60, 381
BbvI GCAGC 4 cut(s) 172, 452, 736, 764
BccI CCATC 2 cut(s) 476, 596
BciT130I CCWGG 2 cut(s) 176, 224
BcnI CCSGG 1 cut(s) 311
BcoDI GTCTC 2 cut(s) 411, 631
BfaI CTAG 2 cut(s) 335, 726
BfmI CTRYAG 2 cut(s) 663, 750
BfoI RGCGCY 1 cut(s) 446
BisI GCNGC 6 cut(s) 161, 438, 441, 446, 750, 753
BlsI GCNGC 6 cut(s) 162, 439, 442, 447, 751, 754
BmcAI AGTACT 1 cut(s) 657
Bme1390I CCNGG 3 cut(s) 176, 224, 311
Bme18I GGWCC 1 cut(s) 684
BmgT120I GGNCC 5 cut(s) 101, 112, 212, 347, 684
BmiI GGNNCC 2 cut(s) 75, 103
BmrFI CCNGG 3 cut(s) 176, 224, 311
BpiI GAAGAC 2 cut(s) 225, 466
Bpu14I TTCGAA 1 cut(s) 593
BpuMI CCSGG 1 cut(s) 311
BsaHI GRCGYC 2 cut(s) 27, 512
BsaJI CCNNGG 1 cut(s) 309
BsaWI WCCGGW 2 cut(s) 8, 76
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 110, 181, 356
Bse1I ACTGG 1 cut(s) 198
BseBI CCWGG 2 cut(s) 176, 224
BseDI CCNNGG 1 cut(s) 309
BseGI GGATG 1 cut(s) 487
BseLI CCNNNNNNNGG 4 cut(s) 109, 110, 181, 356
BseNI ACTGG 1 cut(s) 198
BseRI GAGGAG 1 cut(s) 649
BseSI GKGCMC 1 cut(s) 534
BseXI GCAGC 4 cut(s) 172, 452, 736, 764
BshFI GGCC 5 cut(s) 102, 114, 197, 213, 348
BsiHKAI GWGCWC 2 cut(s) 60, 381
BsiSI CCGG 4 cut(s) 9, 38, 77, 311
BslI CCNNNNNNNGG 4 cut(s) 109, 110, 181, 356
BsmAI GTCTC 2 cut(s) 411, 631
BsnI GGCC 5 cut(s) 102, 114, 197, 213, 348
Bsp119I TTCGAA 1 cut(s) 593
Bsp1286I GDGCHC 4 cut(s) 60, 309, 381, 534
Bsp143I GATC 4 cut(s) 73, 292, 620, 691
BspACI CCGC 2 cut(s) 437, 446
BspANI GGCC 5 cut(s) 102, 114, 197, 213, 348
BspLI GGNNCC 2 cut(s) 75, 103
BspMAI CTGCAG 1 cut(s) 754
BspPI GGATC 4 cut(s) 68, 81, 287, 628
BspQI GCTCTTC 1 cut(s) 297
BspT104I TTCGAA 1 cut(s) 593
BsrI ACTGG 1 cut(s) 198
BssECI CCNNGG 1 cut(s) 309
BssMI GATC 4 cut(s) 73, 292, 620, 691
BssNI GRCGYC 2 cut(s) 27, 512
Bst2UI CCWGG 2 cut(s) 176, 224
Bst4CI ACNGT 1 cut(s) 130
Bst6I CTCTTC 4 cut(s) 242, 260, 297, 701
BstACI GRCGYC 2 cut(s) 27, 512
BstBI TTCGAA 1 cut(s) 593
BstC8I GCNNGC 1 cut(s) 466
BstF5I GGATG 1 cut(s) 487
BstH2I RGCGCY 1 cut(s) 446
BstHHI GCGC 1 cut(s) 445
BstKTI GATC 4 cut(s) 76, 295, 623, 694
BstMAI GTCTC 2 cut(s) 411, 631
BstMBI GATC 4 cut(s) 73, 292, 620, 691
BstMWI GCNNNNNNNGC 3 cut(s) 210, 369, 758
BstNI CCWGG 2 cut(s) 176, 224
BstNSI RCATGY 1 cut(s) 452
BstSCI CCNGG 3 cut(s) 174, 222, 309
BstSFI CTRYAG 2 cut(s) 663, 750
BstSLI GKGCMC 1 cut(s) 534
BstV1I GCAGC 4 cut(s) 172, 452, 736, 764
BstV2I GAAGAC 2 cut(s) 225, 466
BstX2I RGATCY 2 cut(s) 73, 620
BstXI CCANNNNNNTGG 1 cut(s) 205
BstYI RGATCY 2 cut(s) 73, 620
BsuRI GGCC 5 cut(s) 102, 114, 197, 213, 348
BtsCI GGATG 1 cut(s) 487
BtsIMutI CAGTG 3 cut(s) 205, 288, 502
Cac8I GCNNGC 1 cut(s) 466
CfoI GCGC 1 cut(s) 445
Cfr13I GGNCC 5 cut(s) 101, 112, 212, 347, 684
CseI GACGC 1 cut(s) 520
Csp6I GTAC 3 cut(s) 49, 391, 656
CviAII CATG 3 cut(s) 181, 449, 478
CviQI GTAC 3 cut(s) 49, 391, 656
DpnI GATC 4 cut(s) 75, 294, 622, 693
DpnII GATC 4 cut(s) 73, 292, 620, 691
EaeI YGGCCR 1 cut(s) 195
Eam1104I CTCTTC 4 cut(s) 242, 260, 297, 701
EarI CTCTTC 4 cut(s) 242, 260, 297, 701
Ecl136II GAGCTC 1 cut(s) 58
Eco24I GRGCYC 2 cut(s) 60, 309
Eco47I GGWCC 1 cut(s) 684
Eco53kI GAGCTC 1 cut(s) 58
Eco57I CTGAAG 1 cut(s) 237
EcoICRI GAGCTC 1 cut(s) 58
EcoO109I RGGNCCY 1 cut(s) 212
EcoRII CCWGG 2 cut(s) 174, 222
EcoT38I GRGCYC 2 cut(s) 60, 309
FaeI CATG 3 cut(s) 184, 452, 481
FatI CATG 3 cut(s) 180, 448, 477
Fnu4HI GCNGC 6 cut(s) 161, 438, 441, 446, 750, 753
FokI GGATG 1 cut(s) 494
FriOI GRGCYC 2 cut(s) 60, 309
Fsp4HI GCNGC 6 cut(s) 161, 438, 441, 446, 750, 753
FspBI CTAG 2 cut(s) 335, 726
GlaI GCGC 1 cut(s) 444
GluI GCNGC 6 cut(s) 161, 438, 441, 446, 750, 753
HaeII RGCGCY 1 cut(s) 446
HaeIII GGCC 5 cut(s) 102, 114, 197, 213, 348
HapII CCGG 4 cut(s) 9, 38, 77, 311
HgaI GACGC 1 cut(s) 520
HhaI GCGC 1 cut(s) 445
Hin1I GRCGYC 2 cut(s) 27, 512
Hin1II CATG 3 cut(s) 184, 452, 481
Hin6I GCGC 1 cut(s) 443
HinP1I GCGC 1 cut(s) 443
HincII GTYRAC 2 cut(s) 31, 670
HindII GTYRAC 2 cut(s) 31, 670
HinfI GANTC 2 cut(s) 429, 639
HpaI GTTAAC 1 cut(s) 670
HpaII CCGG 4 cut(s) 9, 38, 77, 311
HphI GGTGA 2 cut(s) 23, 35
Hpy166II GTNNAC 3 cut(s) 31, 133, 670
Hpy188I TCNGA 1 cut(s) 301
Hpy188III TCNNGA 3 cut(s) 265, 608, 736
Hpy8I GTNNAC 3 cut(s) 31, 133, 670
Hpy99I CGWCG 1 cut(s) 514
HpyAV CCTTC 5 cut(s) 100, 306, 395, 419, 467
HpyCH4III ACNGT 1 cut(s) 130
HpyCH4IV ACGT 2 cut(s) 27, 33
HpyCH4V TGCA 2 cut(s) 626, 752
HpyF10VI GCNNNNNNNGC 3 cut(s) 210, 369, 758
HpySE526I ACGT 2 cut(s) 27, 33
Hsp92I GRCGYC 2 cut(s) 27, 512
Hsp92II CATG 3 cut(s) 184, 452, 481
HspAI GCGC 1 cut(s) 443
KspAI GTTAAC 1 cut(s) 670
Kzo9I GATC 4 cut(s) 73, 292, 620, 691
LguI GCTCTTC 1 cut(s) 297
LmnI GCTCC 1 cut(s) 384
Lsp1109I GCAGC 4 cut(s) 172, 452, 736, 764
MaeI CTAG 2 cut(s) 335, 726
MaeII ACGT 2 cut(s) 27, 33
MaeIII GTNAC 3 cut(s) 23, 79, 497
MalI GATC 4 cut(s) 75, 294, 622, 693
MboI GATC 4 cut(s) 73, 292, 620, 691
MflI RGATCY 2 cut(s) 73, 620
MhlI GDGCHC 4 cut(s) 60, 309, 381, 534
MluCI AATT 4 cut(s) 142, 246, 269, 516
MlyI GAGTC 1 cut(s) 633
MseI TTAA 2 cut(s) 579, 669
MspI CCGG 4 cut(s) 9, 38, 77, 311
MspR9I CCNGG 3 cut(s) 176, 224, 311
MvaI CCWGG 2 cut(s) 176, 224
MwoI GCNNNNNNNGC 3 cut(s) 210, 369, 758
NciI CCSGG 1 cut(s) 311
NdeII GATC 4 cut(s) 73, 292, 620, 691
NlaIII CATG 3 cut(s) 184, 452, 481
NlaIV GGNNCC 2 cut(s) 75, 103
NmeAIII GCCGAG 3 cut(s) 78, 173, 324
NmuCI GTSAC 2 cut(s) 23, 497
NspI RCATGY 1 cut(s) 452
NspV TTCGAA 1 cut(s) 593
PciSI GCTCTTC 1 cut(s) 297
PfeI GAWTC 1 cut(s) 429
PkrI GCNGC 6 cut(s) 162, 439, 442, 447, 751, 754
PleI GAGTC 1 cut(s) 633
PpsI GAGTC 1 cut(s) 633
Psp124BI GAGCTC 1 cut(s) 60
Psp6I CCWGG 2 cut(s) 174, 222
PspGI CCWGG 2 cut(s) 174, 222
PspN4I GGNNCC 2 cut(s) 75, 103
PspPI GGNCC 5 cut(s) 101, 112, 212, 347, 684
PstI CTGCAG 1 cut(s) 754
PsuI RGATCY 2 cut(s) 73, 620
RsaI GTAC 3 cut(s) 50, 392, 657
RsaNI GTAC 3 cut(s) 49, 391, 656
SacI GAGCTC 1 cut(s) 60
SapI GCTCTTC 1 cut(s) 297
SaqAI TTAA 2 cut(s) 579, 669
SatI GCNGC 6 cut(s) 161, 438, 441, 446, 750, 753
Sau3AI GATC 4 cut(s) 73, 292, 620, 691
Sau96I GGNCC 5 cut(s) 101, 112, 212, 347, 684
ScaI AGTACT 1 cut(s) 657
SchI GAGTC 1 cut(s) 633
ScrFI CCNGG 3 cut(s) 176, 224, 311
SduI GDGCHC 4 cut(s) 60, 309, 381, 534
SfcI CTRYAG 2 cut(s) 663, 750
SfuI TTCGAA 1 cut(s) 593
SinI GGWCC 1 cut(s) 684
Sse9I AATT 4 cut(s) 142, 246, 269, 516
SsiI CCGC 2 cut(s) 437, 446
SspI AATATT 1 cut(s) 743
SspMI CTAG 2 cut(s) 335, 726
SstI GAGCTC 1 cut(s) 60
StyD4I CCNGG 3 cut(s) 174, 222, 309
TaaI ACNGT 1 cut(s) 130
TaiI ACGT 2 cut(s) 30, 36
TaqI TCGA 4 cut(s) 266, 318, 509, 593
TasI AATT 4 cut(s) 142, 246, 269, 516
TatI WGTACW 1 cut(s) 655
TauI GCSGC 2 cut(s) 440, 448
TfiI GAWTC 1 cut(s) 429
Tru1I TTAA 2 cut(s) 579, 669
Tru9I TTAA 2 cut(s) 579, 669
TscAI CASTG 3 cut(s) 205, 295, 502
TseFI GTSAC 2 cut(s) 23, 497
TseI GCWGC 4 cut(s) 160, 440, 749, 752
Tsp45I GTSAC 2 cut(s) 23, 497
TspDTI ATGAA 3 cut(s) 30, 355, 466
TspRI CASTG 3 cut(s) 205, 295, 502
VpaK11BI GGWCC 1 cut(s) 684
XapI RAATTY 1 cut(s) 269
XceI RCATGY 1 cut(s) 452
XspI CTAG 2 cut(s) 335, 726
ZraI GACGTC 1 cut(s) 28
ZrmI AGTACT 1 cut(s) 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.