MD07G1273800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005000
Physical Location & Seq
Forward (+)
33955530 .. 33956315
786 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1273800.v1.1.491

Sequence Viewer

Length: 786 bp
ATGTCAGGTGGGGTTGGTCCAACCTGCAGTGACATTAGCCTCCCTAAAGAACAAGAACATGAATTCAAGGAACACAACGACCAAACCTCCTCAAAACTCACCTCCCATCAGAAGTTCCCAACTTTACACTCCAGCGCCACCGCCTCTCCGCGGAAAGCCGGTGGCGGTATGTTCTCCTTCCGGCAGCTCAACGCCCTCGCCGTCGTCGTCGTCTTCTCCGCCAGCGGCATGGTAAGTCCCCAAGACTTTGCCTTCGTTGTCTTCTCCATGATCTACATGAACTTCATCTCCAAAGTTGCATTCCCCGCCCTCAGTTCCTCTAAAGACCCCACAGTTTTCAACCCCAAAAACAAAATCCTCCGCCTCTACGTCCTAACGGGCGCCATCATCGGCCTTCTGCTCCCCATTGCCTACATTTTCGAGGGCATTTTCGAAGGCGATAAAGAAGGCATCAGCGCCGCTTCTCCGCACGTTTTTCTCCTCGCAAGTCAGGTGTTCATGGAAGGAATGGCTTTCGGCGACCGGTTCTCAACGCCCATACGTGTTTTCGTGCCGGTTTTCTATAATTCCAGGAGGATATTCACCATTGTTGAGTGGCTGAAGAGTGAGTTTTCCAAAGAGTATGAGGAGTACGGTGGATCTGCAAGAAGATTGTACGTTGGGAGAGGACTTGCTATTGCTAATATGGGGTTTTGGTGCTTCAATTTATTTGGGTTCTTGTTGCCTTTCTATCTTCCTAGGGCTTTCAAGAAGTATTACTCTGCCCATAAACTGAAAGATTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

262

Amino Acids

29.21

Weight (kDa)

9.32

Isoelectric Point (pI)

40.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7733 PF24867 58 - 253 4.4e-94 Domain of unknown function (DUF7733)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 32
AccB1I GGYRCC 1 cut(s) 380
AccII CGCG 1 cut(s) 151
AclWI GGATC 1 cut(s) 646
AcsI RAATTY 1 cut(s) 62
AcuI CTGAAG 1 cut(s) 620
AcyI GRCGYC 1 cut(s) 381
AfaI GTAC 2 cut(s) 632, 656
AfiI CCNNNNNNNGG 1 cut(s) 150
AflIII ACRYGT 1 cut(s) 541
AgeI ACCGGT 1 cut(s) 522
AgsI TTSAA 4 cut(s) 67, 340, 703, 748
AjnI CCWGG 1 cut(s) 569
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
AloI GAACNNNNNNTCC 2 cut(s) 272, 304
AluBI AGCT 1 cut(s) 187
AluI AGCT 1 cut(s) 187
AlwI GGATC 1 cut(s) 646
AoxI GGCC 1 cut(s) 391
ApeKI GCWGC 1 cut(s) 184
ApoI RAATTY 1 cut(s) 62
ArsI GACNNNNNNTTYG 2 cut(s) 236, 268
AsiGI ACCGGT 1 cut(s) 522
AspA2I CCTAGG 1 cut(s) 737
AspLEI GCGC 3 cut(s) 137, 383, 458
AspS9I GGNCC 1 cut(s) 17
AsuHPI GGTGA 2 cut(s) 91, 574
AsuII TTCGAA 1 cut(s) 432
AvaII GGWCC 1 cut(s) 17
AvrII CCTAGG 1 cut(s) 737
BanI GGYRCC 1 cut(s) 380
BbsI GAAGAC 2 cut(s) 205, 253
BbvI GCAGC 1 cut(s) 196
BccI CCATC 2 cut(s) 114, 392
BceAI ACGGC 1 cut(s) 185
BciT130I CCWGG 1 cut(s) 571
BfaI CTAG 1 cut(s) 738
BfmI CTRYAG 1 cut(s) 25
BfoI RGCGCY 3 cut(s) 138, 384, 459
BfuAI ACCTGC 1 cut(s) 32
BisI GCNGC 3 cut(s) 185, 226, 459
BlnI CCTAGG 1 cut(s) 737
BlsI GCNGC 3 cut(s) 186, 227, 460
Bme1390I CCNGG 1 cut(s) 571
Bme18I GGWCC 1 cut(s) 17
BmgT120I GGNCC 1 cut(s) 17
BmiI GGNNCC 1 cut(s) 382
BmrFI CCNGG 1 cut(s) 571
BmsI GCATC 1 cut(s) 459
BpiI GAAGAC 2 cut(s) 205, 253
BpmI CTGGAG 1 cut(s) 115
Bpu14I TTCGAA 1 cut(s) 432
BsaAI YACGTR 1 cut(s) 542
BsaHI GRCGYC 1 cut(s) 381
BsaJI CCNNGG 2 cut(s) 149, 737
BsaWI WCCGGW 1 cut(s) 522
BsaXI ACNNNNNCTCC 6 cut(s) 71, 101, 130, 160, 272, 302
Bsc4I CCNNNNNNNGG 1 cut(s) 150
Bse118I RCCGGY 3 cut(s) 158, 522, 553
Bse3DI GCAATG 1 cut(s) 405
BseBI CCWGG 1 cut(s) 571
BseDI CCNNGG 2 cut(s) 149, 737
BseLI CCNNNNNNNGG 1 cut(s) 150
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 1 cut(s) 325
BseRI GAGGAG 3 cut(s) 79, 470, 641
BseXI GCAGC 1 cut(s) 196
Bsh1236I CGCG 1 cut(s) 151
Bsh1285I CGRYCG 1 cut(s) 523
BshFI GGCC 1 cut(s) 393
BshNI GGYRCC 1 cut(s) 380
BshTI ACCGGT 1 cut(s) 522
BsiEI CGRYCG 1 cut(s) 523
BsiSI CCGG 4 cut(s) 159, 181, 523, 554
BslFI GGGAC 1 cut(s) 222
BslI CCNNNNNNNGG 1 cut(s) 150
BsmFI GGGAC 1 cut(s) 222
BsmI GAATGC 1 cut(s) 299
BsnI GGCC 1 cut(s) 393
Bsp119I TTCGAA 1 cut(s) 432
Bsp143I GATC 2 cut(s) 270, 638
BspANI GGCC 1 cut(s) 393
BspCNI CTCAG 1 cut(s) 324
BspFNI CGCG 1 cut(s) 151
BspLI GGNNCC 1 cut(s) 382
BspMAI CTGCAG 1 cut(s) 29
BspMI ACCTGC 1 cut(s) 32
BspPI GGATC 1 cut(s) 646
BspT104I TTCGAA 1 cut(s) 432
BspT107I GGYRCC 1 cut(s) 380
BsrDI GCAATG 1 cut(s) 405
BsrFI RCCGGY 3 cut(s) 158, 522, 553
BssAI RCCGGY 3 cut(s) 158, 522, 553
BssECI CCNNGG 2 cut(s) 149, 737
BssMI GATC 2 cut(s) 270, 638
BssNI GRCGYC 1 cut(s) 381
BssT1I CCWWGG 1 cut(s) 737
Bst2UI CCWGG 1 cut(s) 571
Bst4CI ACNGT 2 cut(s) 334, 635
Bst6I CTCTTC 1 cut(s) 596
BstACI GRCGYC 1 cut(s) 381
BstBAI YACGTR 1 cut(s) 542
BstBI TTCGAA 1 cut(s) 432
BstC8I GCNNGC 1 cut(s) 223
BstDEI CTNAG 1 cut(s) 311
BstDSI CCRYGG 1 cut(s) 149
BstFNI CGCG 1 cut(s) 151
BstH2I RGCGCY 3 cut(s) 138, 384, 459
BstHHI GCGC 3 cut(s) 137, 383, 458
BstKTI GATC 2 cut(s) 273, 641
BstMBI GATC 2 cut(s) 270, 638
BstMCI CGRYCG 1 cut(s) 523
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstNI CCWGG 1 cut(s) 571
BstSCI CCNGG 1 cut(s) 569
BstSFI CTRYAG 1 cut(s) 25
BstUI CGCG 1 cut(s) 151
BstV1I GCAGC 1 cut(s) 196
BstV2I GAAGAC 2 cut(s) 205, 253
BstX2I RGATCY 1 cut(s) 638
BstXI CCANNNNNNTGG 1 cut(s) 229
BstYI RGATCY 1 cut(s) 638
BsuRI GGCC 1 cut(s) 393
BtgI CCRYGG 1 cut(s) 149
BtsI GCAGTG 1 cut(s) 34
BtsIMutI CAGTG 1 cut(s) 34
BveI ACCTGC 1 cut(s) 32
Cac8I GCNNGC 1 cut(s) 223
CfoI GCGC 3 cut(s) 137, 383, 458
Cfr10I RCCGGY 3 cut(s) 158, 522, 553
Cfr13I GGNCC 1 cut(s) 17
Cfr42I CCGCGG 1 cut(s) 152
Csp6I GTAC 2 cut(s) 631, 655
CspAI ACCGGT 1 cut(s) 522
CviAII CATG 5 cut(s) 59, 229, 268, 277, 499
CviJI RGCY 7 cut(s) 39, 158, 187, 393, 512, 598, 743
CviKI_1 RGCY 7 cut(s) 39, 158, 187, 393, 512, 598, 743
CviQI GTAC 2 cut(s) 631, 655
DdeI CTNAG 1 cut(s) 311
DinI GGCGCC 1 cut(s) 382
DpnI GATC 2 cut(s) 272, 640
DpnII GATC 2 cut(s) 270, 638
Eam1104I CTCTTC 1 cut(s) 596
EarI CTCTTC 1 cut(s) 596
EciI GGCGGA 2 cut(s) 208, 350
Eco130I CCWWGG 1 cut(s) 737
Eco47I GGWCC 1 cut(s) 17
Eco57I CTGAAG 1 cut(s) 620
EcoRI GAATTC 1 cut(s) 62
EcoRII CCWGG 1 cut(s) 569
EcoT14I CCWWGG 1 cut(s) 737
EgeI GGCGCC 1 cut(s) 382
EheI GGCGCC 1 cut(s) 382
ErhI CCWWGG 1 cut(s) 737
FaeI CATG 5 cut(s) 62, 232, 271, 280, 502
FaqI GGGAC 1 cut(s) 222
FatI CATG 5 cut(s) 58, 228, 267, 276, 498
FauI CCCGC 1 cut(s) 313
Fnu4HI GCNGC 3 cut(s) 185, 226, 459
Fsp4HI GCNGC 3 cut(s) 185, 226, 459
FspBI CTAG 1 cut(s) 738
GlaI GCGC 3 cut(s) 136, 382, 457
GluI GCNGC 3 cut(s) 185, 226, 459
GsuI CTGGAG 1 cut(s) 115
HaeII RGCGCY 3 cut(s) 138, 384, 459
HaeIII GGCC 1 cut(s) 393
HapII CCGG 4 cut(s) 159, 181, 523, 554
HhaI GCGC 3 cut(s) 137, 383, 458
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 5 cut(s) 62, 232, 271, 280, 502
Hin6I GCGC 3 cut(s) 135, 381, 456
HinP1I GCGC 3 cut(s) 135, 381, 456
HpaII CCGG 4 cut(s) 159, 181, 523, 554
HphI GGTGA 2 cut(s) 91, 574
Hpy188I TCNGA 1 cut(s) 111
Hpy188III TCNNGA 1 cut(s) 748
Hpy99I CGWCG 3 cut(s) 206, 209, 212
HpyAV CCTTC 6 cut(s) 187, 262, 404, 428, 440, 497
HpyCH4III ACNGT 2 cut(s) 334, 635
HpyCH4IV ACGT 4 cut(s) 369, 471, 541, 657
HpyCH4V TGCA 3 cut(s) 27, 299, 644
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpyF3I CTNAG 1 cut(s) 311
HpySE526I ACGT 4 cut(s) 369, 471, 541, 657
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 5 cut(s) 62, 232, 271, 280, 502
HspAI GCGC 3 cut(s) 135, 381, 456
KasI GGCGCC 1 cut(s) 380
KspI CCGCGG 1 cut(s) 152
Kzo9I GATC 2 cut(s) 270, 638
LmnI GCTCC 1 cut(s) 405
Lsp1109I GCAGC 1 cut(s) 196
LweI GCATC 1 cut(s) 459
MaeI CTAG 1 cut(s) 738
MaeII ACGT 4 cut(s) 369, 471, 541, 657
MaeIII GTNAC 1 cut(s) 29
MalI GATC 2 cut(s) 272, 640
MboI GATC 2 cut(s) 270, 638
MboII GAAGA 5 cut(s) 205, 253, 613, 660, 725
MflI RGATCY 1 cut(s) 638
MluCI AATT 3 cut(s) 62, 565, 703
Mly113I GGCGCC 1 cut(s) 381
MmeI TCCRAC 1 cut(s) 44
MspA1I CMGCKG 2 cut(s) 151, 225
MspI CCGG 4 cut(s) 159, 181, 523, 554
MspR9I CCNGG 1 cut(s) 571
Mva1269I GAATGC 1 cut(s) 299
MvaI CCWGG 1 cut(s) 571
MvnI CGCG 1 cut(s) 151
MwoI GCNNNNNNNGC 1 cut(s) 305
NarI GGCGCC 1 cut(s) 381
NdeII GATC 2 cut(s) 270, 638
NlaIII CATG 5 cut(s) 62, 232, 271, 280, 502
NlaIV GGNNCC 1 cut(s) 382
NmuCI GTSAC 1 cut(s) 29
NspV TTCGAA 1 cut(s) 432
PcsI WCGNNNNNNNCGW 2 cut(s) 198, 204
PctI GAATGC 1 cut(s) 299
PfoI TCCNGGA 1 cut(s) 569
PinAI ACCGGT 1 cut(s) 522
PkrI GCNGC 3 cut(s) 186, 227, 460
PluTI GGCGCC 1 cut(s) 384
Ppu21I YACGTR 1 cut(s) 542
Psp6I CCWGG 1 cut(s) 569
PspGI CCWGG 1 cut(s) 569
PspN4I GGNNCC 1 cut(s) 382
PspPI GGNCC 1 cut(s) 17
PstI CTGCAG 1 cut(s) 29
PsuI RGATCY 1 cut(s) 638
RsaI GTAC 2 cut(s) 632, 656
RsaNI GTAC 2 cut(s) 631, 655
SacII CCGCGG 1 cut(s) 152
SatI GCNGC 3 cut(s) 185, 226, 459
Sau3AI GATC 2 cut(s) 270, 638
Sau96I GGNCC 1 cut(s) 17
ScrFI CCNGG 1 cut(s) 571
SfaNI GCATC 1 cut(s) 459
SfcI CTRYAG 1 cut(s) 25
SfoI GGCGCC 1 cut(s) 382
Sfr303I CCGCGG 1 cut(s) 152
SfuI TTCGAA 1 cut(s) 432
SgrBI CCGCGG 1 cut(s) 152
SinI GGWCC 1 cut(s) 17
Sse9I AATT 3 cut(s) 62, 565, 703
SspDI GGCGCC 1 cut(s) 380
SspMI CTAG 1 cut(s) 738
StyD4I CCNGG 1 cut(s) 569
StyI CCWWGG 1 cut(s) 737
TaaI ACNGT 2 cut(s) 334, 635
TaiI ACGT 4 cut(s) 372, 474, 544, 660
TaqI TCGA 2 cut(s) 420, 432
TasI AATT 3 cut(s) 62, 565, 703
TauI GCSGC 2 cut(s) 228, 461
TscAI CASTG 1 cut(s) 34
TseFI GTSAC 1 cut(s) 29
TseI GCWGC 1 cut(s) 184
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 4 cut(s) 75, 274, 293, 487
TspRI CASTG 1 cut(s) 34
VpaK11BI GGWCC 1 cut(s) 17
XapI RAATTY 1 cut(s) 62
XmaJI CCTAGG 1 cut(s) 737
XspI CTAG 1 cut(s) 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.