pycom07g25020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005000
Physical Location & Seq
Forward (+)
25473009 .. 25473398
390 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g25020.1

Sequence Viewer

Length: 390 bp
ATGGTCAAAACCGGCCCGGCTTTTGGCGCCGTGTTGGGCCTCGCCCTGCCGATAGCGTATATATTTCACGGGGTTTTGGAAGGAGACCAAGAAGGCATCAAAGCTGCTGTCCCGCACGTGTTTTTACTTGCTAGTCAAGCTTTTATGGACGGAGTGGCGTATTCCGATAGGTTTTCGACGCCGATTAGGGTTTTCATGCCGGTTTTCTTTAACGCAAAGAGGATTTTCACGCTTATGGATTGGGTTGGGAACGAATTCTCCAAAGTGTGTGTTGGTGGTGGTTGTGACGCAGCTGCAGAAAGAAGAGTGCTTCTTGGAAGAGGACTTGCCGTTGCTAACTTGGTGTTTTGGAGTTTAGATTTTTTTGTTAAAATAATTTTTGGATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

130

Amino Acids

14.04

Weight (kDa)

7.81

Isoelectric Point (pI)

22.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7733 PF24867 7 - 126 8.5e-47 Domain of unknown function (DUF7733)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 26
AciI CCGC 1 cut(s) 113
AcsI RAATTY 1 cut(s) 254
AcvI CACGTG 1 cut(s) 118
AcyI GRCGYC 2 cut(s) 27, 179
AfiI CCNNNNNNNGG 1 cut(s) 23
AflIII ACRYGT 1 cut(s) 117
AloI GAACNNNNNNTCC 2 cut(s) 242, 274
AluBI AGCT 3 cut(s) 104, 140, 293
AluI AGCT 3 cut(s) 104, 140, 293
Alw26I GTCTC 1 cut(s) 78
AoxI GGCC 2 cut(s) 13, 37
ApeKI GCWGC 3 cut(s) 104, 290, 293
ApoI RAATTY 1 cut(s) 254
ArsI GACNNNNNNTTYG 2 cut(s) 93, 125
Asp700I GAANNNNTTC 1 cut(s) 254
AspLEI GCGC 1 cut(s) 29
AspS9I GGNCC 2 cut(s) 14, 37
AsuC2I CCSGG 1 cut(s) 17
BanI GGYRCC 1 cut(s) 26
BbrPI CACGTG 1 cut(s) 118
BbvI GCAGC 3 cut(s) 91, 280, 302
BceAI ACGGC 2 cut(s) 14, 314
BcnI CCSGG 1 cut(s) 17
BcoDI GTCTC 1 cut(s) 78
BfaI CTAG 1 cut(s) 132
BfmI CTRYAG 1 cut(s) 294
BfoI RGCGCY 1 cut(s) 30
BisI GCNGC 3 cut(s) 105, 291, 294
BlsI GCNGC 3 cut(s) 106, 292, 295
Bme1390I CCNGG 1 cut(s) 17
BmgT120I GGNCC 2 cut(s) 14, 37
BmiI GGNNCC 1 cut(s) 28
BmrFI CCNGG 1 cut(s) 17
BmsI GCATC 1 cut(s) 105
BpuMI CCSGG 1 cut(s) 17
BsaAI YACGTR 1 cut(s) 118
BsaHI GRCGYC 2 cut(s) 27, 179
BsaI GGTCTC 1 cut(s) 78
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
Bsc4I CCNNNNNNNGG 1 cut(s) 23
Bse118I RCCGGY 2 cut(s) 11, 199
BseLI CCNNNNNNNGG 1 cut(s) 23
BseXI GCAGC 3 cut(s) 91, 280, 302
BshFI GGCC 2 cut(s) 15, 39
BshNI GGYRCC 1 cut(s) 26
BsiSI CCGG 3 cut(s) 12, 17, 200
BslFI GGGAC 1 cut(s) 95
BslI CCNNNNNNNGG 1 cut(s) 23
BsmAI GTCTC 1 cut(s) 78
BsmFI GGGAC 1 cut(s) 95
BsnI GGCC 2 cut(s) 15, 39
Bso31I GGTCTC 1 cut(s) 78
BspACI CCGC 1 cut(s) 113
BspANI GGCC 2 cut(s) 15, 39
BspLI GGNNCC 1 cut(s) 28
BspMAI CTGCAG 1 cut(s) 298
BspT107I GGYRCC 1 cut(s) 26
BspTNI GGTCTC 1 cut(s) 78
BsrFI RCCGGY 2 cut(s) 11, 199
BssAI RCCGGY 2 cut(s) 11, 199
BssNI GRCGYC 2 cut(s) 27, 179
Bst6I CTCTTC 2 cut(s) 298, 313
BstACI GRCGYC 2 cut(s) 27, 179
BstBAI YACGTR 1 cut(s) 118
BstH2I RGCGCY 1 cut(s) 30
BstHHI GCGC 1 cut(s) 29
BstMAI GTCTC 1 cut(s) 78
BstMWI GCNNNNNNNGC 2 cut(s) 26, 137
BstSCI CCNGG 1 cut(s) 15
BstSFI CTRYAG 1 cut(s) 294
BstV1I GCAGC 3 cut(s) 91, 280, 302
BsuRI GGCC 2 cut(s) 15, 39
CfoI GCGC 1 cut(s) 29
Cfr10I RCCGGY 2 cut(s) 11, 199
Cfr13I GGNCC 2 cut(s) 14, 37
CseI GACGC 2 cut(s) 187, 296
CviAII CATG 1 cut(s) 196
CviJI RGCY 6 cut(s) 15, 20, 39, 104, 140, 293
CviKI_1 RGCY 6 cut(s) 15, 20, 39, 104, 140, 293
DinI GGCGCC 1 cut(s) 28
Eam1104I CTCTTC 2 cut(s) 298, 313
EarI CTCTTC 2 cut(s) 298, 313
Eco31I GGTCTC 1 cut(s) 78
Eco72I CACGTG 1 cut(s) 118
EcoRI GAATTC 1 cut(s) 254
EgeI GGCGCC 1 cut(s) 28
EheI GGCGCC 1 cut(s) 28
FaeI CATG 1 cut(s) 199
FaiI YATR 5 cut(s) 60, 62, 146, 197, 236
FaqI GGGAC 1 cut(s) 95
FatI CATG 1 cut(s) 195
FauI CCCGC 1 cut(s) 120
Fnu4HI GCNGC 3 cut(s) 105, 291, 294
Fsp4HI GCNGC 3 cut(s) 105, 291, 294
FspBI CTAG 1 cut(s) 132
GlaI GCGC 1 cut(s) 28
GluI GCNGC 3 cut(s) 105, 291, 294
HaeII RGCGCY 1 cut(s) 30
HaeIII GGCC 2 cut(s) 15, 39
HapII CCGG 3 cut(s) 12, 17, 200
HgaI GACGC 2 cut(s) 187, 296
HhaI GCGC 1 cut(s) 29
Hin1I GRCGYC 2 cut(s) 27, 179
Hin1II CATG 1 cut(s) 199
Hin6I GCGC 1 cut(s) 27
HinP1I GCGC 1 cut(s) 27
HindIII AAGCTT 1 cut(s) 138
HpaII CCGG 3 cut(s) 12, 17, 200
Hpy188I TCNGA 1 cut(s) 166
Hpy99I CGWCG 1 cut(s) 181
HpyAV CCTTC 2 cut(s) 74, 86
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 1 cut(s) 296
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 137
HpySE526I ACGT 1 cut(s) 117
Hsp92I GRCGYC 2 cut(s) 27, 179
Hsp92II CATG 1 cut(s) 199
HspAI GCGC 1 cut(s) 27
KasI GGCGCC 1 cut(s) 26
LpnPI CCDG 4 cut(s) 25, 30, 59, 213
Lsp1109I GCAGC 3 cut(s) 91, 280, 302
LweI GCATC 1 cut(s) 105
MaeI CTAG 1 cut(s) 132
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 1 cut(s) 284
MboII GAAGA 2 cut(s) 315, 330
MluCI AATT 2 cut(s) 254, 375
Mly113I GGCGCC 1 cut(s) 27
MnlI CCTC 3 cut(s) 50, 213, 314
MroXI GAANNNNTTC 1 cut(s) 254
MseI TTAA 2 cut(s) 210, 369
MslI CAYNNNNRTG 1 cut(s) 233
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 3 cut(s) 12, 17, 200
MspR9I CCNGG 1 cut(s) 17
MwoI GCNNNNNNNGC 2 cut(s) 26, 137
NarI GGCGCC 1 cut(s) 27
NciI CCSGG 1 cut(s) 17
NlaIII CATG 1 cut(s) 199
NlaIV GGNNCC 1 cut(s) 28
NmuCI GTSAC 1 cut(s) 284
PdmI GAANNNNTTC 1 cut(s) 254
PkrI GCNGC 3 cut(s) 106, 292, 295
PluTI GGCGCC 1 cut(s) 30
PmaCI CACGTG 1 cut(s) 118
PmlI CACGTG 1 cut(s) 118
Ppu21I YACGTR 1 cut(s) 118
PspCI CACGTG 1 cut(s) 118
PspN4I GGNNCC 1 cut(s) 28
PspPI GGNCC 2 cut(s) 14, 37
PstI CTGCAG 1 cut(s) 298
PvuII CAGCTG 1 cut(s) 293
RseI CAYNNNNRTG 1 cut(s) 233
SaqAI TTAA 2 cut(s) 210, 369
SatI GCNGC 3 cut(s) 105, 291, 294
Sau96I GGNCC 2 cut(s) 14, 37
ScrFI CCNGG 1 cut(s) 17
SetI ASST 5 cut(s) 106, 120, 142, 173, 295
SfaNI GCATC 1 cut(s) 105
SfcI CTRYAG 1 cut(s) 294
SfoI GGCGCC 1 cut(s) 28
SmiMI CAYNNNNRTG 1 cut(s) 233
Sse9I AATT 2 cut(s) 254, 375
SsiI CCGC 1 cut(s) 113
SspDI GGCGCC 1 cut(s) 26
SspMI CTAG 1 cut(s) 132
StyD4I CCNGG 1 cut(s) 15
TaiI ACGT 1 cut(s) 120
TaqI TCGA 1 cut(s) 176
TasI AATT 2 cut(s) 254, 375
Tru1I TTAA 2 cut(s) 210, 369
Tru9I TTAA 2 cut(s) 210, 369
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 3 cut(s) 104, 290, 293
Tsp45I GTSAC 1 cut(s) 284
TspDTI ATGAA 1 cut(s) 184
TspGWI ACGGA 1 cut(s) 165
XapI RAATTY 1 cut(s) 254
XmnI GAANNNNTTC 1 cut(s) 254
XspI CTAG 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.