MD01G1229200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0006092
Physical Location & Seq
Forward (+)
31871474 .. 31872798
1325 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1229200.v1.1.491

Sequence Viewer

Length: 558 bp
ATGGAGGGCGAAACCGGAATCGACGTCAAAGTGATTAATCAGCCGTCGTCGACGCCATCGCGCTTGAAGAAGAAGAAGAGAGTGACAGCCCAGAAATCGCCCCAGAAGGCTCCGCCCTCTTCTTCCGCGGAGGACGACGACGGGAGTCCAATCAGGCAAATTCTCTGTGTCAAAAACAAGGTCGATGTTAAACACTTCGAGGACGTCGAGGACTGCTTCATTTTGGATTTCGACCCCTCGGAGCCGGTGCAGTTGTCCAAGTTGTCAGTGTCGGTTAATGGTGGTGGTGGTCACGATGGTTCTCCCGATATCGCTGTTGTTGCTGAGAAAGGACAGGTTGCTTGTAGAGACTATCCACATTCAAGGCACCTCTGCCTGAAATTTCCTTTCGGGACGACACCCCATGAGAGCTACTGTGATCTGTGCTACTGCTACGTTTGTGATTCTGCCGCTCCATGTGGGTTATGGAAACCATCGCATTGCCATGCCGCTGCGCACATTGGTGATTGGAAGTCCAAAAGGATGTTGAGGAAGCAGCAAGCCGCGGTGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

186

Amino Acids

20.28

Weight (kDa)

8.29

Isoelectric Point (pI)

58.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 27, 207
Acc16I TGCGCA 1 cut(s) 495
AccB1I GGYRCC 1 cut(s) 366
AccBSI CCGCTC 1 cut(s) 452
AccI GTMKAC 1 cut(s) 50
AccII CGCG 3 cut(s) 61, 128, 545
AciI CCGC 7 cut(s) 113, 126, 128, 450, 489, 543, 545
AcsI RAATTY 2 cut(s) 159, 380
AcyI GRCGYC 3 cut(s) 24, 53, 204
AgsI TTSAA 2 cut(s) 67, 363
AleI CACNNNNGTG 1 cut(s) 501
AluBI AGCT 1 cut(s) 411
AluI AGCT 1 cut(s) 411
Alw26I GTCTC 1 cut(s) 342
ApeKI GCWGC 2 cut(s) 491, 535
ApoI RAATTY 2 cut(s) 159, 380
ArsI GACNNNNNNTTYG 2 cut(s) 165, 197
AseI ATTAAT 1 cut(s) 36
AspLEI GCGC 2 cut(s) 63, 496
AsuHPI GGTGA 1 cut(s) 515
BanI GGYRCC 1 cut(s) 366
BbvI GCAGC 2 cut(s) 478, 547
BccI CCATC 3 cut(s) 64, 290, 481
BceAI ACGGC 1 cut(s) 28
BcoDI GTCTC 1 cut(s) 342
BisI GCNGC 5 cut(s) 450, 489, 492, 536, 543
BlsI GCNGC 5 cut(s) 451, 490, 493, 537, 544
BmiI GGNNCC 3 cut(s) 111, 243, 368
BoxI GACNNNNGTC 1 cut(s) 144
BsaHI GRCGYC 3 cut(s) 24, 53, 204
BsaJI CCNNGG 3 cut(s) 126, 237, 543
BsaWI WCCGGW 1 cut(s) 14
Bse118I RCCGGY 1 cut(s) 244
Bse3DI GCAATG 1 cut(s) 478
BseDI CCNNGG 3 cut(s) 126, 237, 543
BseGI GGATG 1 cut(s) 528
BseMI GCAATG 1 cut(s) 478
BseMII CTCAG 1 cut(s) 315
BseXI GCAGC 2 cut(s) 478, 547
BsgI GTGCAG 1 cut(s) 269
Bsh1236I CGCG 3 cut(s) 61, 128, 545
BshNI GGYRCC 1 cut(s) 366
BsiSI CCGG 2 cut(s) 15, 245
BslFI GGGAC 1 cut(s) 406
BsmAI GTCTC 1 cut(s) 342
BsmFI GGGAC 1 cut(s) 406
Bsp143I GATC 1 cut(s) 418
BspACI CCGC 7 cut(s) 113, 126, 128, 450, 489, 543, 545
BspCNI CTCAG 1 cut(s) 316
BspFNI CGCG 3 cut(s) 61, 128, 545
BspLI GGNNCC 3 cut(s) 111, 243, 368
BspT107I GGYRCC 1 cut(s) 366
BsrBI CCGCTC 1 cut(s) 452
BsrDI GCAATG 1 cut(s) 478
BsrFI RCCGGY 1 cut(s) 244
BssAI RCCGGY 1 cut(s) 244
BssECI CCNNGG 3 cut(s) 126, 237, 543
BssMI GATC 1 cut(s) 418
BssNI GRCGYC 3 cut(s) 24, 53, 204
Bst4CI ACNGT 1 cut(s) 416
Bst6I CTCTTC 2 cut(s) 71, 124
BstACI GRCGYC 3 cut(s) 24, 53, 204
BstC8I GCNNGC 1 cut(s) 540
BstDEI CTNAG 1 cut(s) 324
BstDSI CCRYGG 2 cut(s) 126, 543
BstF5I GGATG 1 cut(s) 528
BstFNI CGCG 3 cut(s) 61, 128, 545
BstHHI GCGC 2 cut(s) 63, 496
BstKTI GATC 1 cut(s) 421
BstMAI GTCTC 1 cut(s) 342
BstMBI GATC 1 cut(s) 418
BstMWI GCNNNNNNNGC 1 cut(s) 320
BstPAI GACNNNNGTC 1 cut(s) 144
BstUI CGCG 3 cut(s) 61, 128, 545
BstV1I GCAGC 2 cut(s) 478, 547
BtgI CCRYGG 2 cut(s) 126, 543
BtgZI GCGATG 2 cut(s) 42, 459
BtsCI GGATG 1 cut(s) 528
BtsIMutI CAGTG 1 cut(s) 273
Cac8I GCNNGC 1 cut(s) 540
CfoI GCGC 2 cut(s) 63, 496
Cfr10I RCCGGY 1 cut(s) 244
Cfr42I CCGCGG 2 cut(s) 129, 546
CseI GACGC 1 cut(s) 61
CviAII CATG 3 cut(s) 404, 456, 485
CviJI RGCY 6 cut(s) 43, 89, 110, 244, 411, 542
CviKI_1 RGCY 6 cut(s) 43, 89, 110, 244, 411, 542
DdeI CTNAG 1 cut(s) 324
DpnI GATC 1 cut(s) 420
DpnII GATC 1 cut(s) 418
Eam1104I CTCTTC 2 cut(s) 71, 124
EarI CTCTTC 2 cut(s) 71, 124
EciI GGCGGA 1 cut(s) 102
Eco32I GATATC 1 cut(s) 310
EcoRV GATATC 1 cut(s) 310
FaeI CATG 3 cut(s) 407, 459, 488
FaiI YATR 4 cut(s) 405, 457, 466, 486
FaqI GGGAC 1 cut(s) 406
FatI CATG 3 cut(s) 403, 455, 484
FblI GTMKAC 1 cut(s) 50
Fnu4HI GCNGC 5 cut(s) 450, 489, 492, 536, 543
FokI GGATG 1 cut(s) 535
Fsp4HI GCNGC 5 cut(s) 450, 489, 492, 536, 543
FspI TGCGCA 1 cut(s) 495
GlaI GCGC 2 cut(s) 62, 495
GluI GCNGC 5 cut(s) 450, 489, 492, 536, 543
HapII CCGG 2 cut(s) 15, 245
HgaI GACGC 1 cut(s) 61
HhaI GCGC 2 cut(s) 63, 496
Hin1I GRCGYC 3 cut(s) 24, 53, 204
Hin1II CATG 3 cut(s) 407, 459, 488
Hin6I GCGC 2 cut(s) 61, 494
HinP1I GCGC 2 cut(s) 61, 494
HincII GTYRAC 1 cut(s) 51
HindII GTYRAC 1 cut(s) 51
HinfI GANTC 3 cut(s) 18, 145, 443
HpaII CCGG 2 cut(s) 15, 245
HphI GGTGA 1 cut(s) 515
Hpy166II GTNNAC 1 cut(s) 51
Hpy188I TCNGA 1 cut(s) 241
Hpy188III TCNNGA 3 cut(s) 293, 305, 391
Hpy8I GTNNAC 1 cut(s) 51
Hpy99I CGWCG 7 cut(s) 26, 49, 52, 55, 140, 143, 209
HpyAV CCTTC 1 cut(s) 100
HpyCH4III ACNGT 1 cut(s) 416
HpyCH4IV ACGT 3 cut(s) 24, 204, 435
HpyCH4V TGCA 1 cut(s) 250
HpyF10VI GCNNNNNNNGC 1 cut(s) 320
HpyF3I CTNAG 1 cut(s) 324
HpySE526I ACGT 3 cut(s) 24, 204, 435
Hsp92I GRCGYC 3 cut(s) 24, 53, 204
Hsp92II CATG 3 cut(s) 407, 459, 488
HspAI GCGC 2 cut(s) 61, 494
KspI CCGCGG 2 cut(s) 129, 546
Kzo9I GATC 1 cut(s) 418
LmnI GCTCC 3 cut(s) 115, 241, 457
LpnPI CCDG 7 cut(s) 28, 104, 116, 139, 258, 320, 389
Lsp1109I GCAGC 2 cut(s) 478, 547
MaeII ACGT 3 cut(s) 24, 204, 435
MaeIII GTNAC 2 cut(s) 82, 290
MalI GATC 1 cut(s) 420
MbiI CCGCTC 1 cut(s) 452
MboI GATC 1 cut(s) 418
MboII GAAGA 6 cut(s) 79, 82, 85, 88, 111, 114
MluCI AATT 2 cut(s) 159, 380
MlyI GAGTC 1 cut(s) 154
MnlI CCTC 7 cut(s) 124, 127, 193, 202, 247, 380, 522
MseI TTAA 3 cut(s) 36, 189, 276
MslI CAYNNNNRTG 2 cut(s) 483, 501
MspA1I CMGCKG 3 cut(s) 128, 491, 545
MspI CCGG 2 cut(s) 15, 245
MvnI CGCG 3 cut(s) 61, 128, 545
MwoI GCNNNNNNNGC 1 cut(s) 320
NdeII GATC 1 cut(s) 418
NlaIII CATG 3 cut(s) 407, 459, 488
NlaIV GGNNCC 3 cut(s) 111, 243, 368
NmuCI GTSAC 2 cut(s) 82, 290
NsbI TGCGCA 1 cut(s) 495
OliI CACNNNNGTG 1 cut(s) 501
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PfeI GAWTC 2 cut(s) 18, 443
PkrI GCNGC 5 cut(s) 451, 490, 493, 537, 544
PleI GAGTC 1 cut(s) 153
PpsI GAGTC 1 cut(s) 153
PshAI GACNNNNGTC 1 cut(s) 144
PshBI ATTAAT 1 cut(s) 36
PspN4I GGNNCC 3 cut(s) 111, 243, 368
RseI CAYNNNNRTG 2 cut(s) 483, 501
SacII CCGCGG 2 cut(s) 129, 546
SalI GTCGAC 1 cut(s) 49
SaqAI TTAA 3 cut(s) 36, 189, 276
SatI GCNGC 5 cut(s) 450, 489, 492, 536, 543
Sau3AI GATC 1 cut(s) 418
SchI GAGTC 1 cut(s) 154
SetI ASST 7 cut(s) 27, 183, 207, 339, 372, 413, 438
Sfr303I CCGCGG 2 cut(s) 129, 546
SgrBI CCGCGG 2 cut(s) 129, 546
SgrDI CGTCGACG 1 cut(s) 49
SmiMI CAYNNNNRTG 2 cut(s) 483, 501
Sse9I AATT 2 cut(s) 159, 380
SsiI CCGC 7 cut(s) 113, 126, 128, 450, 489, 543, 545
TaaI ACNGT 1 cut(s) 416
TaiI ACGT 3 cut(s) 27, 207, 438
TaqI TCGA 6 cut(s) 21, 50, 183, 198, 207, 231
TasI AATT 2 cut(s) 159, 380
TauI GCSGC 3 cut(s) 452, 491, 545
TfiI GAWTC 2 cut(s) 18, 443
Tru1I TTAA 3 cut(s) 36, 189, 276
Tru9I TTAA 3 cut(s) 36, 189, 276
TscAI CASTG 1 cut(s) 273
TseFI GTSAC 2 cut(s) 82, 290
TseI GCWGC 2 cut(s) 491, 535
Tsp45I GTSAC 2 cut(s) 82, 290
TspDTI ATGAA 1 cut(s) 208
TspRI CASTG 1 cut(s) 273
VspI ATTAAT 1 cut(s) 36
XapI RAATTY 2 cut(s) 159, 380
XcmI CCANNNNNNNNNTGG 1 cut(s) 462
XmiI GTMKAC 1 cut(s) 50
ZraI GACGTC 2 cut(s) 25, 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.