pycom07g19510

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006092
Physical Location & Seq
Reverse (-)
21861270 .. 21862330
1061 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g19510.1

Sequence Viewer

Length: 546 bp
ATGAGCGAAATCGGAATCGGTGTCAAAGCGATTAATCTGTCGTCGACGACGCCGTCGCGCGAGAAGAAGGTAGTGACAACCGAGAAAACTCCCCAGAATGCTCCGTCGTCTTCTTCCGCGGAGGACGAGGACGGGAGTCCAATCAGGCACATTGTCTGTGTCAAAAACAAGGTCGATGTCAAACACTTTGAGGACTTCGAGGACTGCTTCATCTTGGATTTCGACCCCTTTGAGCCGGTGCAGTCCAAGTTGACCGTGACGGATAATGGTGGTGGTGGTCACGCTGGTTCTCCCGATATCGCTGTTGTTGCTGAGAAAGGACAGGTTGCTTGTAGAGACTATCCACATTCAAGACACCTCTGCCTGAAATTTCCTTTCGAGACGACACCCCATGAGAGCTACTGTGAGCTGTGCTACTGCTATGTTTGCGATTCTGCTGCTCCATGCATGTTGTGGAAACTATCACATTGCCACGCCGCTGCGCACATTGGTGACTGGAAGTGCAAAAGGATGTTGATGAAGCAGCAAGCCGCGGTGAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

182

Amino Acids

19.99

Weight (kDa)

6.29

Isoelectric Point (pI)

62.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 52
Acc16I TGCGCA 1 cut(s) 483
AccI GTMKAC 1 cut(s) 44
AccII CGCG 4 cut(s) 58, 60, 119, 533
AciI CCGC 5 cut(s) 117, 119, 477, 531, 533
AcsI RAATTY 1 cut(s) 368
AcyI GRCGYC 1 cut(s) 50
AgsI TTSAA 1 cut(s) 351
AleI CACNNNNGTG 1 cut(s) 489
AluBI AGCT 2 cut(s) 399, 409
AluI AGCT 2 cut(s) 399, 409
Alw26I GTCTC 2 cut(s) 330, 374
ApeKI GCWGC 3 cut(s) 437, 479, 523
ApoI RAATTY 1 cut(s) 368
ArsI GACNNNNNNTTYG 2 cut(s) 156, 188
AseI ATTAAT 1 cut(s) 33
AspLEI GCGC 2 cut(s) 60, 484
AsuHPI GGTGA 1 cut(s) 503
BbsI GAAGAC 1 cut(s) 102
BbvI GCAGC 3 cut(s) 424, 466, 535
BceAI ACGGC 1 cut(s) 37
BcgI CGANNNNNNTGC 2 cut(s) 419, 453
BcoDI GTCTC 2 cut(s) 330, 374
BisI GCNGC 5 cut(s) 438, 477, 480, 524, 531
BlsI GCNGC 5 cut(s) 439, 478, 481, 525, 532
BoxI GACNNNNGTC 1 cut(s) 135
BpiI GAAGAC 1 cut(s) 102
BsaHI GRCGYC 1 cut(s) 50
BsaJI CCNNGG 2 cut(s) 117, 531
Bse118I RCCGGY 1 cut(s) 235
Bse1I ACTGG 1 cut(s) 500
Bse3DI GCAATG 1 cut(s) 466
BseDI CCNNGG 2 cut(s) 117, 531
BseGI GGATG 1 cut(s) 516
BseMI GCAATG 1 cut(s) 466
BseMII CTCAG 1 cut(s) 303
BseNI ACTGG 1 cut(s) 500
BseXI GCAGC 3 cut(s) 424, 466, 535
BsgI GTGCAG 1 cut(s) 260
Bsh1236I CGCG 4 cut(s) 58, 60, 119, 533
BsiSI CCGG 1 cut(s) 236
BsmAI GTCTC 2 cut(s) 330, 374
BsmBI CGTCTC 1 cut(s) 374
BsmI GAATGC 1 cut(s) 103
BspACI CCGC 5 cut(s) 117, 119, 477, 531, 533
BspCNI CTCAG 1 cut(s) 304
BspFNI CGCG 4 cut(s) 58, 60, 119, 533
BsrDI GCAATG 1 cut(s) 466
BsrFI RCCGGY 1 cut(s) 235
BsrI ACTGG 1 cut(s) 500
BssAI RCCGGY 1 cut(s) 235
BssECI CCNNGG 2 cut(s) 117, 531
BssNI GRCGYC 1 cut(s) 50
Bst4CI ACNGT 2 cut(s) 256, 404
BstACI GRCGYC 1 cut(s) 50
BstC8I GCNNGC 1 cut(s) 528
BstDEI CTNAG 1 cut(s) 312
BstDSI CCRYGG 2 cut(s) 117, 531
BstF5I GGATG 1 cut(s) 516
BstFNI CGCG 4 cut(s) 58, 60, 119, 533
BstHHI GCGC 2 cut(s) 60, 484
BstMAI GTCTC 2 cut(s) 330, 374
BstMWI GCNNNNNNNGC 2 cut(s) 308, 426
BstNSI RCATGY 1 cut(s) 451
BstPAI GACNNNNGTC 1 cut(s) 135
BstUI CGCG 4 cut(s) 58, 60, 119, 533
BstV1I GCAGC 3 cut(s) 424, 466, 535
BstV2I GAAGAC 1 cut(s) 102
BtgI CCRYGG 2 cut(s) 117, 531
BtsCI GGATG 1 cut(s) 516
Cac8I GCNNGC 1 cut(s) 528
CfoI GCGC 2 cut(s) 60, 484
Cfr10I RCCGGY 1 cut(s) 235
Cfr42I CCGCGG 2 cut(s) 120, 534
CseI GACGC 1 cut(s) 58
CviAII CATG 3 cut(s) 392, 444, 448
CviJI RGCY 4 cut(s) 235, 399, 409, 530
CviKI_1 RGCY 4 cut(s) 235, 399, 409, 530
DdeI CTNAG 1 cut(s) 312
DrdI GACNNNNNNGTC 1 cut(s) 52
DseDI GACNNNNNNGTC 1 cut(s) 52
Eco32I GATATC 1 cut(s) 298
EcoRV GATATC 1 cut(s) 298
EcoT22I ATGCAT 1 cut(s) 449
Esp3I CGTCTC 1 cut(s) 374
FaeI CATG 3 cut(s) 395, 447, 451
FaiI YATR 4 cut(s) 393, 423, 445, 449
FatI CATG 3 cut(s) 391, 443, 447
FblI GTMKAC 1 cut(s) 44
Fnu4HI GCNGC 5 cut(s) 438, 477, 480, 524, 531
FokI GGATG 1 cut(s) 523
Fsp4HI GCNGC 5 cut(s) 438, 477, 480, 524, 531
FspI TGCGCA 1 cut(s) 483
GlaI GCGC 2 cut(s) 59, 483
GluI GCNGC 5 cut(s) 438, 477, 480, 524, 531
HapII CCGG 1 cut(s) 236
HgaI GACGC 1 cut(s) 58
HhaI GCGC 2 cut(s) 60, 484
Hin1I GRCGYC 1 cut(s) 50
Hin1II CATG 3 cut(s) 395, 447, 451
Hin6I GCGC 2 cut(s) 58, 482
HinP1I GCGC 2 cut(s) 58, 482
HincII GTYRAC 2 cut(s) 45, 252
HindII GTYRAC 2 cut(s) 45, 252
HinfI GANTC 3 cut(s) 15, 136, 431
HpaII CCGG 1 cut(s) 236
HphI GGTGA 1 cut(s) 503
Hpy166II GTNNAC 2 cut(s) 45, 252
Hpy188I TCNGA 1 cut(s) 14
Hpy188III TCNNGA 3 cut(s) 293, 351, 379
Hpy8I GTNNAC 2 cut(s) 45, 252
Hpy99I CGWCG 5 cut(s) 46, 49, 52, 58, 109
HpyAV CCTTC 1 cut(s) 61
HpyCH4III ACNGT 2 cut(s) 256, 404
HpyCH4V TGCA 3 cut(s) 241, 447, 504
HpyF10VI GCNNNNNNNGC 2 cut(s) 308, 426
HpyF3I CTNAG 1 cut(s) 312
Hsp92I GRCGYC 1 cut(s) 50
Hsp92II CATG 3 cut(s) 395, 447, 451
HspAI GCGC 2 cut(s) 58, 482
KspI CCGCGG 2 cut(s) 120, 534
LmnI GCTCC 2 cut(s) 106, 445
LpnPI CCDG 7 cut(s) 107, 130, 249, 270, 308, 377, 481
Lsp1109I GCAGC 3 cut(s) 424, 466, 535
MaeIII GTNAC 4 cut(s) 73, 256, 278, 491
MboII GAAGA 3 cut(s) 76, 102, 105
MluCI AATT 1 cut(s) 368
MlyI GAGTC 1 cut(s) 145
MnlI CCTC 5 cut(s) 115, 121, 184, 193, 368
Mph1103I ATGCAT 1 cut(s) 449
MseI TTAA 1 cut(s) 33
MslI CAYNNNNRTG 1 cut(s) 489
MspA1I CMGCKG 3 cut(s) 119, 479, 533
MspI CCGG 1 cut(s) 236
Mva1269I GAATGC 1 cut(s) 103
MvnI CGCG 4 cut(s) 58, 60, 119, 533
MwoI GCNNNNNNNGC 2 cut(s) 308, 426
NlaIII CATG 3 cut(s) 395, 447, 451
NmuCI GTSAC 4 cut(s) 73, 256, 278, 491
NsbI TGCGCA 1 cut(s) 483
NsiI ATGCAT 1 cut(s) 449
NspI RCATGY 1 cut(s) 451
OliI CACNNNNGTG 1 cut(s) 489
PcsI WCGNNNNNNNCGW 1 cut(s) 50
PctI GAATGC 1 cut(s) 103
PfeI GAWTC 2 cut(s) 15, 431
PflFI GACNNNGTC 1 cut(s) 52
PkrI GCNGC 5 cut(s) 439, 478, 481, 525, 532
PleI GAGTC 1 cut(s) 144
PpsI GAGTC 1 cut(s) 144
PshAI GACNNNNGTC 1 cut(s) 135
PshBI ATTAAT 1 cut(s) 33
PsyI GACNNNGTC 1 cut(s) 52
RseI CAYNNNNRTG 1 cut(s) 489
SacII CCGCGG 2 cut(s) 120, 534
SalI GTCGAC 1 cut(s) 43
SaqAI TTAA 1 cut(s) 33
SatI GCNGC 5 cut(s) 438, 477, 480, 524, 531
SchI GAGTC 1 cut(s) 145
SetI ASST 6 cut(s) 72, 174, 327, 360, 401, 411
Sfr303I CCGCGG 2 cut(s) 120, 534
SgrBI CCGCGG 2 cut(s) 120, 534
SgrDI CGTCGACG 1 cut(s) 43
SmiMI CAYNNNNRTG 1 cut(s) 489
Sse9I AATT 1 cut(s) 368
SsiI CCGC 5 cut(s) 117, 119, 477, 531, 533
TaaI ACNGT 2 cut(s) 256, 404
TaqI TCGA 5 cut(s) 44, 174, 198, 222, 378
TasI AATT 1 cut(s) 368
TauI GCSGC 2 cut(s) 479, 533
TfiI GAWTC 2 cut(s) 15, 431
Tru1I TTAA 1 cut(s) 33
Tru9I TTAA 1 cut(s) 33
TseFI GTSAC 4 cut(s) 73, 256, 278, 491
TseI GCWGC 3 cut(s) 437, 479, 523
Tsp45I GTSAC 4 cut(s) 73, 256, 278, 491
TspDTI ATGAA 2 cut(s) 199, 533
TspGWI ACGGA 2 cut(s) 93, 275
Tth111I GACNNNGTC 1 cut(s) 52
VspI ATTAAT 1 cut(s) 33
XapI RAATTY 1 cut(s) 368
XceI RCATGY 1 cut(s) 451
XcmI CCANNNNNNNNNTGG 1 cut(s) 450
XmiI GTMKAC 1 cut(s) 44
Zsp2I ATGCAT 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.