pycom07g27340

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006092
Physical Location & Seq
Forward (+)
27111069 .. 27112126
1058 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g27340.3

Sequence Viewer

Length: 543 bp
ATGAGCGAAATCGGAATCGATGTCAAAGCGATTAATCTGTCGTCGACGACGCCGTCGCGCGAGAAGAAGGGGGTGACAGCCGAGAAAACTCCCCAGAAAGCTCCGTCGTCTTCTTCCGCGGAGGACGAGGACGGGAGTCCGATCAAGCACATTGTCTGTGTCAAAAACAAGGTCGATGTCAAACATTTTGAGGACGTCGAGGACTGCTTCATCTTGGATTTCGACCCCTTTGAGCCGGTGCAGTTTTCCAAGTTGTCCGTGTCGGATAATGGTGGTGGTGGCGGTTCTCCCGATATCGCTGTTGTTGCTGAGAAAGGACAGGTTGCTTGTAGAGACTATCCACATTCAAGACACCTCTGCCTGAAATTTCCTTTCGAGACGACACCCCATGAGAGCTACTGTGAGCTGTGCTACTGCTATGTTTGCGATTCTGCCGCTCCATGCGGGTTGTGGAAACTGTCACATTGCCATGCTGCTGCGCACATTGGTGACTGGGAGTGCAAAAGGATGTTGATGAAGCAGCAAGCCGCGATGAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

19.78

Weight (kDa)

5.89

Isoelectric Point (pI)

59.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 52
AatII GACGTC 1 cut(s) 198
Acc16I TGCGCA 1 cut(s) 480
AccBSI CCGCTC 1 cut(s) 437
AccI GTMKAC 1 cut(s) 44
AccII CGCG 4 cut(s) 58, 60, 119, 530
AciI CCGC 6 cut(s) 117, 119, 282, 435, 444, 528
AcsI RAATTY 1 cut(s) 365
AcyI GRCGYC 2 cut(s) 50, 195
AgsI TTSAA 1 cut(s) 348
AleI CACNNNNGTG 1 cut(s) 486
AluBI AGCT 3 cut(s) 101, 396, 406
AluI AGCT 3 cut(s) 101, 396, 406
Alw26I GTCTC 2 cut(s) 327, 371
ApeKI GCWGC 3 cut(s) 473, 476, 520
ApoI RAATTY 1 cut(s) 365
ArsI GACNNNNNNTTYG 2 cut(s) 156, 188
AseI ATTAAT 1 cut(s) 33
AspLEI GCGC 2 cut(s) 60, 481
AsuHPI GGTGA 2 cut(s) 85, 500
BbsI GAAGAC 1 cut(s) 102
BbvI GCAGC 3 cut(s) 460, 463, 532
BceAI ACGGC 1 cut(s) 37
BcoDI GTCTC 2 cut(s) 327, 371
BisI GCNGC 5 cut(s) 435, 474, 477, 521, 528
BlsI GCNGC 5 cut(s) 436, 475, 478, 522, 529
BmrI ACTGGG 1 cut(s) 502
BmuI ACTGGG 1 cut(s) 502
BoxI GACNNNNGTC 1 cut(s) 135
BpiI GAAGAC 1 cut(s) 102
Bsa29I ATCGAT 1 cut(s) 18
BsaHI GRCGYC 2 cut(s) 50, 195
BsaJI CCNNGG 1 cut(s) 117
Bse118I RCCGGY 1 cut(s) 235
Bse1I ACTGG 1 cut(s) 497
Bse3DI GCAATG 1 cut(s) 463
BseCI ATCGAT 1 cut(s) 18
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 1 cut(s) 513
BseMI GCAATG 1 cut(s) 463
BseMII CTCAG 1 cut(s) 300
BseNI ACTGG 1 cut(s) 497
BseXI GCAGC 3 cut(s) 460, 463, 532
BsgI GTGCAG 1 cut(s) 260
Bsh1236I CGCG 4 cut(s) 58, 60, 119, 530
BshVI ATCGAT 1 cut(s) 18
BsiSI CCGG 1 cut(s) 236
BsmAI GTCTC 2 cut(s) 327, 371
BsmBI CGTCTC 1 cut(s) 371
Bsp143I GATC 1 cut(s) 141
BspACI CCGC 6 cut(s) 117, 119, 282, 435, 444, 528
BspCNI CTCAG 1 cut(s) 301
BspDI ATCGAT 1 cut(s) 18
BspFNI CGCG 4 cut(s) 58, 60, 119, 530
BsrBI CCGCTC 1 cut(s) 437
BsrDI GCAATG 1 cut(s) 463
BsrFI RCCGGY 1 cut(s) 235
BsrI ACTGG 1 cut(s) 497
BssAI RCCGGY 1 cut(s) 235
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 1 cut(s) 141
BssNI GRCGYC 2 cut(s) 50, 195
Bst4CI ACNGT 2 cut(s) 401, 459
BstACI GRCGYC 2 cut(s) 50, 195
BstC8I GCNNGC 1 cut(s) 525
BstDEI CTNAG 1 cut(s) 309
BstDSI CCRYGG 1 cut(s) 117
BstF5I GGATG 1 cut(s) 513
BstFNI CGCG 4 cut(s) 58, 60, 119, 530
BstHHI GCGC 2 cut(s) 60, 481
BstKTI GATC 1 cut(s) 144
BstMAI GTCTC 2 cut(s) 327, 371
BstMBI GATC 1 cut(s) 141
BstMWI GCNNNNNNNGC 2 cut(s) 305, 423
BstPAI GACNNNNGTC 1 cut(s) 135
BstUI CGCG 4 cut(s) 58, 60, 119, 530
BstV1I GCAGC 3 cut(s) 460, 463, 532
BstV2I GAAGAC 1 cut(s) 102
Bsu15I ATCGAT 1 cut(s) 18
BsuTUI ATCGAT 1 cut(s) 18
BtgI CCRYGG 1 cut(s) 117
BtsCI GGATG 1 cut(s) 513
Cac8I GCNNGC 1 cut(s) 525
CfoI GCGC 2 cut(s) 60, 481
Cfr10I RCCGGY 1 cut(s) 235
Cfr42I CCGCGG 1 cut(s) 120
ClaI ATCGAT 1 cut(s) 18
CseI GACGC 1 cut(s) 58
CviAII CATG 3 cut(s) 389, 441, 470
CviJI RGCY 6 cut(s) 80, 101, 235, 396, 406, 527
CviKI_1 RGCY 6 cut(s) 80, 101, 235, 396, 406, 527
DdeI CTNAG 1 cut(s) 309
DpnI GATC 1 cut(s) 143
DpnII GATC 1 cut(s) 141
DrdI GACNNNNNNGTC 1 cut(s) 52
DseDI GACNNNNNNGTC 1 cut(s) 52
Eco32I GATATC 1 cut(s) 295
EcoRV GATATC 1 cut(s) 295
Esp3I CGTCTC 1 cut(s) 371
FaeI CATG 3 cut(s) 392, 444, 473
FaiI YATR 4 cut(s) 390, 420, 442, 471
FatI CATG 3 cut(s) 388, 440, 469
FauI CCCGC 1 cut(s) 437
FblI GTMKAC 1 cut(s) 44
Fnu4HI GCNGC 5 cut(s) 435, 474, 477, 521, 528
FokI GGATG 1 cut(s) 520
Fsp4HI GCNGC 5 cut(s) 435, 474, 477, 521, 528
FspI TGCGCA 1 cut(s) 480
GlaI GCGC 2 cut(s) 59, 480
GluI GCNGC 5 cut(s) 435, 474, 477, 521, 528
HapII CCGG 1 cut(s) 236
HgaI GACGC 1 cut(s) 58
HhaI GCGC 2 cut(s) 60, 481
Hin1I GRCGYC 2 cut(s) 50, 195
Hin1II CATG 3 cut(s) 392, 444, 473
Hin6I GCGC 2 cut(s) 58, 479
HinP1I GCGC 2 cut(s) 58, 479
HincII GTYRAC 1 cut(s) 45
HindII GTYRAC 1 cut(s) 45
HinfI GANTC 3 cut(s) 15, 136, 428
HpaII CCGG 1 cut(s) 236
HphI GGTGA 2 cut(s) 85, 500
Hpy166II GTNNAC 1 cut(s) 45
Hpy188I TCNGA 3 cut(s) 14, 141, 265
Hpy188III TCNNGA 3 cut(s) 290, 348, 376
Hpy8I GTNNAC 1 cut(s) 45
Hpy99I CGWCG 6 cut(s) 46, 49, 52, 58, 109, 200
HpyAV CCTTC 1 cut(s) 61
HpyCH4III ACNGT 2 cut(s) 401, 459
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 2 cut(s) 241, 501
HpyF10VI GCNNNNNNNGC 2 cut(s) 305, 423
HpyF3I CTNAG 1 cut(s) 309
HpySE526I ACGT 1 cut(s) 195
Hsp92I GRCGYC 2 cut(s) 50, 195
Hsp92II CATG 3 cut(s) 392, 444, 473
HspAI GCGC 2 cut(s) 58, 479
KspI CCGCGG 1 cut(s) 120
Kzo9I GATC 1 cut(s) 141
LmnI GCTCC 2 cut(s) 106, 442
LpnPI CCDG 5 cut(s) 107, 249, 305, 374, 478
Lsp1109I GCAGC 3 cut(s) 460, 463, 532
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 3 cut(s) 73, 459, 488
MalI GATC 1 cut(s) 143
MbiI CCGCTC 1 cut(s) 437
MboI GATC 1 cut(s) 141
MboII GAAGA 3 cut(s) 76, 102, 105
MluCI AATT 1 cut(s) 365
MlyI GAGTC 1 cut(s) 145
MmeI TCCRAC 1 cut(s) 243
MnlI CCTC 5 cut(s) 115, 121, 184, 193, 365
MseI TTAA 1 cut(s) 33
MslI CAYNNNNRTG 2 cut(s) 468, 486
MspA1I CMGCKG 1 cut(s) 119
MspI CCGG 1 cut(s) 236
MvnI CGCG 4 cut(s) 58, 60, 119, 530
MwoI GCNNNNNNNGC 2 cut(s) 305, 423
NdeII GATC 1 cut(s) 141
NlaIII CATG 3 cut(s) 392, 444, 473
NmeAIII GCCGAG 1 cut(s) 106
NmuCI GTSAC 3 cut(s) 73, 459, 488
NsbI TGCGCA 1 cut(s) 480
OliI CACNNNNGTG 1 cut(s) 486
PcsI WCGNNNNNNNCGW 1 cut(s) 50
PfeI GAWTC 2 cut(s) 15, 428
PflFI GACNNNGTC 1 cut(s) 52
PkrI GCNGC 5 cut(s) 436, 475, 478, 522, 529
PleI GAGTC 1 cut(s) 144
PpsI GAGTC 1 cut(s) 144
PshAI GACNNNNGTC 1 cut(s) 135
PshBI ATTAAT 1 cut(s) 33
PsyI GACNNNGTC 1 cut(s) 52
RseI CAYNNNNRTG 2 cut(s) 468, 486
SacII CCGCGG 1 cut(s) 120
SalI GTCGAC 1 cut(s) 43
SaqAI TTAA 1 cut(s) 33
SatI GCNGC 5 cut(s) 435, 474, 477, 521, 528
Sau3AI GATC 1 cut(s) 141
SchI GAGTC 1 cut(s) 145
SetI ASST 7 cut(s) 103, 174, 198, 324, 357, 398, 408
Sfr303I CCGCGG 1 cut(s) 120
SgrBI CCGCGG 1 cut(s) 120
SgrDI CGTCGACG 1 cut(s) 43
SmiMI CAYNNNNRTG 2 cut(s) 468, 486
Sse9I AATT 1 cut(s) 365
SsiI CCGC 6 cut(s) 117, 119, 282, 435, 444, 528
TaaI ACNGT 2 cut(s) 401, 459
TaiI ACGT 1 cut(s) 198
TaqI TCGA 6 cut(s) 18, 44, 174, 198, 222, 375
TasI AATT 1 cut(s) 365
TauI GCSGC 2 cut(s) 437, 530
TfiI GAWTC 2 cut(s) 15, 428
Tru1I TTAA 1 cut(s) 33
Tru9I TTAA 1 cut(s) 33
TseFI GTSAC 3 cut(s) 73, 459, 488
TseI GCWGC 3 cut(s) 473, 476, 520
Tsp45I GTSAC 3 cut(s) 73, 459, 488
TspDTI ATGAA 2 cut(s) 199, 530
TspGWI ACGGA 2 cut(s) 93, 247
Tth111I GACNNNGTC 1 cut(s) 52
VspI ATTAAT 1 cut(s) 33
XapI RAATTY 1 cut(s) 365
XcmI CCANNNNNNNNNTGG 1 cut(s) 447
XmiI GTMKAC 1 cut(s) 44
ZraI GACGTC 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.