Rh1AG463000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0006092
Physical Location & Seq
Forward (+)
67921433 .. 67922435
1003 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG463000.1

Sequence Viewer

Length: 555 bp
ATGAGCAACACCAATGACAAGACTCCGATCGATTTATCCACCCCAACTCCTCCGCCGGAGAAGAAGAACAAGCGGGCCGTCACTAACAAGAAGCGCACTTCGTCCGGCCCAGAAGAACAAGACGACGAGTCTCCGATCAGGCACCACCTCATTGTGACCAAAGAGGAAGATGTGAAGCGTTTTGAGGAGCTCGATGACTGCTTCATTCTGGGTTTCGACCAAGTTGAGTCGATTGAGATGAAGAAGTTGTCTGTGGCGGATGAGGATGCTGGGTCGCCGGAGATCGCCGTTGTTGCCGAGAAAGGCCAGGTGGCTTGCAGAGATTATCCACATCCGAGGCATCTCTGCGTTAAATTTCCGTTTGAGACAACATCGCATGAGACACATTGTGAGCTGTGTTACTGCTATGTGTGCGATACAGCAGCTCCGTGCAAGTCATGGTCATCTTCTAGTAACAATGCACTGCTATCACATTGTGATGCTTATGACAACAGTGGTGTTTGGAAGCGGGAAAGGACATTGAGGAAACAGCTATCAGCTGTGTTTAAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

184

Amino Acids

20.82

Weight (kDa)

5.66

Isoelectric Point (pI)

58.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 141
AciI CCGC 4 cut(s) 53, 73, 257, 508
AcsI RAATTY 1 cut(s) 353
AdeI CACNNNGTG 2 cut(s) 389, 476
AhdI GACNNNNNGTC 1 cut(s) 127
AjnI CCWGG 1 cut(s) 306
AluBI AGCT 5 cut(s) 190, 394, 425, 532, 539
AluI AGCT 5 cut(s) 190, 394, 425, 532, 539
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 3 cut(s) 135, 359, 374
AoxI GGCC 3 cut(s) 75, 106, 304
ApeKI GCWGC 1 cut(s) 422
ApoI RAATTY 1 cut(s) 353
AspLEI GCGC 1 cut(s) 96
AspS9I GGNCC 2 cut(s) 75, 107
BanI GGYRCC 1 cut(s) 141
BanII GRGCYC 1 cut(s) 192
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 1 cut(s) 434
BceAI ACGGC 2 cut(s) 62, 272
BciT130I CCWGG 1 cut(s) 308
BcoDI GTCTC 3 cut(s) 135, 359, 374
BfaI CTAG 1 cut(s) 450
BisI GCNGC 1 cut(s) 423
BlsI GCNGC 1 cut(s) 424
Bme1390I CCNGG 1 cut(s) 308
BmeRI GACNNNNNGTC 1 cut(s) 127
BmgT120I GGNCC 2 cut(s) 75, 107
BmiI GGNNCC 1 cut(s) 143
BmrFI CCNGG 1 cut(s) 308
BmsI GCATC 3 cut(s) 256, 349, 469
Bsa29I ATCGAT 1 cut(s) 30
BsaJI CCNNGG 1 cut(s) 335
BsaXI ACNNNNNCTCC 4 cut(s) 31, 61, 409, 439
BseBI CCWGG 1 cut(s) 308
BseCI ATCGAT 1 cut(s) 30
BseDI CCNNGG 1 cut(s) 335
BseGI GGATG 3 cut(s) 265, 271, 331
BseRI GAGGAG 2 cut(s) 39, 200
BseXI GCAGC 1 cut(s) 434
BseYI CCCAGC 1 cut(s) 269
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 3 cut(s) 77, 108, 306
BshNI GGYRCC 1 cut(s) 141
BshVI ATCGAT 1 cut(s) 30
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 192
BsiSI CCGG 3 cut(s) 56, 105, 278
BsmAI GTCTC 3 cut(s) 135, 359, 374
BsnI GGCC 3 cut(s) 77, 108, 306
Bsp1286I GDGCHC 1 cut(s) 192
Bsp143I GATC 3 cut(s) 27, 135, 282
BspACI CCGC 4 cut(s) 53, 73, 257, 508
BspANI GGCC 3 cut(s) 77, 108, 306
BspDI ATCGAT 1 cut(s) 30
BspLI GGNNCC 1 cut(s) 143
BspT107I GGYRCC 1 cut(s) 141
BssECI CCNNGG 1 cut(s) 335
BssMI GATC 3 cut(s) 27, 135, 282
Bst2UI CCWGG 1 cut(s) 308
Bst4CI ACNGT 1 cut(s) 494
BstC8I GCNNGC 2 cut(s) 75, 316
BstF5I GGATG 3 cut(s) 265, 271, 331
BstHHI GCGC 1 cut(s) 96
BstKTI GATC 3 cut(s) 30, 138, 285
BstMAI GTCTC 3 cut(s) 135, 359, 374
BstMBI GATC 3 cut(s) 27, 135, 282
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 2 cut(s) 293, 411
BstNI CCWGG 1 cut(s) 308
BstSCI CCNGG 1 cut(s) 306
BstV1I GCAGC 1 cut(s) 434
Bsu15I ATCGAT 1 cut(s) 30
BsuRI GGCC 3 cut(s) 77, 108, 306
BsuTUI ATCGAT 1 cut(s) 30
BtgZI GCGATG 1 cut(s) 357
BtsCI GGATG 3 cut(s) 265, 271, 331
BtsI GCAGTG 1 cut(s) 461
BtsIMutI CAGTG 2 cut(s) 461, 499
Cac8I GCNNGC 2 cut(s) 75, 316
CfoI GCGC 1 cut(s) 96
Cfr13I GGNCC 2 cut(s) 75, 107
ClaI ATCGAT 1 cut(s) 30
CviAII CATG 2 cut(s) 377, 438
CviJI RGCY 9 cut(s) 77, 108, 190, 306, 314, 394, 425, 532, 539
CviKI_1 RGCY 9 cut(s) 77, 108, 190, 306, 314, 394, 425, 532, 539
DpnI GATC 3 cut(s) 29, 137, 284
DpnII GATC 3 cut(s) 27, 135, 282
DraIII CACNNNGTG 2 cut(s) 389, 476
DriI GACNNNNNGTC 1 cut(s) 127
Eam1105I GACNNNNNGTC 1 cut(s) 127
EciI GGCGGA 2 cut(s) 42, 272
Ecl136II GAGCTC 1 cut(s) 190
Eco24I GRGCYC 1 cut(s) 192
Eco53kI GAGCTC 1 cut(s) 190
EcoICRI GAGCTC 1 cut(s) 190
EcoRII CCWGG 1 cut(s) 306
EcoT38I GRGCYC 1 cut(s) 192
FaeI CATG 2 cut(s) 380, 441
FaiI YATR 4 cut(s) 378, 408, 439, 486
FatI CATG 2 cut(s) 376, 437
FauI CCCGC 2 cut(s) 66, 501
Fnu4HI GCNGC 1 cut(s) 423
FokI GGATG 3 cut(s) 272, 278, 318
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 1 cut(s) 423
FspBI CTAG 1 cut(s) 450
GlaI GCGC 1 cut(s) 95
GluI GCNGC 1 cut(s) 423
GsaI CCCAGC 1 cut(s) 273
HaeIII GGCC 3 cut(s) 77, 108, 306
HapII CCGG 3 cut(s) 56, 105, 278
HhaI GCGC 1 cut(s) 96
Hin1II CATG 2 cut(s) 380, 441
Hin6I GCGC 1 cut(s) 94
HinP1I GCGC 1 cut(s) 94
HinfI GANTC 3 cut(s) 22, 128, 227
HpaII CCGG 3 cut(s) 56, 105, 278
Hpy188I TCNGA 3 cut(s) 27, 135, 336
Hpy99I CGWCG 1 cut(s) 128
HpyCH4III ACNGT 1 cut(s) 494
HpyCH4V TGCA 3 cut(s) 318, 432, 461
HpyF10VI GCNNNNNNNGC 2 cut(s) 293, 411
Hsp92II CATG 2 cut(s) 380, 441
HspAI GCGC 1 cut(s) 94
Kzo9I GATC 3 cut(s) 27, 135, 282
LmnI GCTCC 2 cut(s) 187, 430
LpnPI CCDG 9 cut(s) 69, 118, 123, 124, 194, 255, 291, 293, 320
Lsp1109I GCAGC 1 cut(s) 434
LweI GCATC 3 cut(s) 256, 349, 469
MaeI CTAG 1 cut(s) 450
MaeIII GTNAC 4 cut(s) 79, 154, 398, 452
MalI GATC 3 cut(s) 29, 137, 284
MboI GATC 3 cut(s) 27, 135, 282
MboII GAAGA 6 cut(s) 73, 76, 125, 179, 253, 438
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 1 cut(s) 353
MlyI GAGTC 3 cut(s) 16, 137, 236
MnlI CCTC 7 cut(s) 60, 157, 158, 178, 256, 330, 516
MseI TTAA 2 cut(s) 351, 546
MslI CAYNNNNRTG 1 cut(s) 477
MspA1I CMGCKG 1 cut(s) 539
MspI CCGG 3 cut(s) 56, 105, 278
MspR9I CCNGG 1 cut(s) 308
MvaI CCWGG 1 cut(s) 308
MwoI GCNNNNNNNGC 2 cut(s) 293, 411
NdeII GATC 3 cut(s) 27, 135, 282
NlaIII CATG 2 cut(s) 380, 441
NlaIV GGNNCC 1 cut(s) 143
NmeAIII GCCGAG 1 cut(s) 322
NmuCI GTSAC 2 cut(s) 79, 154
PkrI GCNGC 1 cut(s) 424
Ple19I CGATCG 1 cut(s) 30
PleI GAGTC 3 cut(s) 16, 136, 235
PpsI GAGTC 3 cut(s) 16, 136, 235
Psp124BI GAGCTC 1 cut(s) 192
Psp6I CCWGG 1 cut(s) 306
PspFI CCCAGC 1 cut(s) 269
PspGI CCWGG 1 cut(s) 306
PspN4I GGNNCC 1 cut(s) 143
PspPI GGNCC 2 cut(s) 75, 107
PvuI CGATCG 1 cut(s) 30
PvuII CAGCTG 1 cut(s) 539
RseI CAYNNNNRTG 1 cut(s) 477
SacI GAGCTC 1 cut(s) 192
SaqAI TTAA 2 cut(s) 351, 546
SatI GCNGC 1 cut(s) 423
Sau3AI GATC 3 cut(s) 27, 135, 282
Sau96I GGNCC 2 cut(s) 75, 107
SchI GAGTC 3 cut(s) 16, 137, 236
ScrFI CCNGG 1 cut(s) 308
SduI GDGCHC 1 cut(s) 192
SetI ASST 7 cut(s) 150, 192, 312, 396, 427, 534, 541
SfaNI GCATC 3 cut(s) 256, 349, 469
SmiMI CAYNNNNRTG 1 cut(s) 477
Sse9I AATT 1 cut(s) 353
SsiI CCGC 4 cut(s) 53, 73, 257, 508
SspMI CTAG 1 cut(s) 450
SstI GAGCTC 1 cut(s) 192
StyD4I CCNGG 1 cut(s) 306
TaaI ACNGT 1 cut(s) 494
TaqI TCGA 4 cut(s) 30, 192, 216, 230
TasI AATT 1 cut(s) 353
Tru1I TTAA 2 cut(s) 351, 546
Tru9I TTAA 2 cut(s) 351, 546
TscAI CASTG 2 cut(s) 468, 499
TseFI GTSAC 2 cut(s) 79, 154
TseI GCWGC 1 cut(s) 422
Tsp45I GTSAC 2 cut(s) 79, 154
TspDTI ATGAA 2 cut(s) 193, 254
TspGWI ACGGA 2 cut(s) 348, 417
TspRI CASTG 2 cut(s) 468, 499
XapI RAATTY 1 cut(s) 353
XspI CTAG 1 cut(s) 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.