Rh1DG452700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0006092
Physical Location & Seq
Forward (+)
65999994 .. 66001056
1063 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG452700.1

Sequence Viewer

Length: 459 bp
ATGCCACCAAAACGCAAAAGCAAACCCCCACAGAGAAGCAGCGAGAGATTGAAGATCAAAATGAGCAACACCAATGACAAGTCTCCGATCGATTTATCCACCCCAACTCCTCCGCCGGAGAAGAAGAACAAGCCGGCCGTCACTAACAAGAAGCCCACTTCGTCCGGCCCAGAAGAACAAGACGACGAGTCTCCGATCAGGCACCACTTCATTGTGACCAAAGAGGAAGATGTGAAGCGTTTTGAGGAGCTCGATGACTGCTTCATTCTGGGTTTCGACCCAGTTGAGTCGATTGAGATGAAGAAGTTGTCTGTGGCGGATACGGCTGCTGGGTCGCCGGAGATCGCCGTTGTTGCCGAGAAAGGCCAGGTGGCTTGCAGAGATTATCCACATCCGAGGCATCTCTGCGTTAAATTTCTGTTTGAGACAACACCGCATGAGACACATTGTGAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

152

Amino Acids

17.14

Weight (kDa)

6.31

Isoelectric Point (pI)

64.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 201
AciI CCGC 3 cut(s) 113, 317, 434
AcoI YGGCCR 1 cut(s) 135
AcsI RAATTY 1 cut(s) 413
AdeI CACNNNGTG 1 cut(s) 449
AgsI TTSAA 1 cut(s) 52
AhdI GACNNNNNGTC 1 cut(s) 187
AjnI CCWGG 1 cut(s) 366
AluBI AGCT 2 cut(s) 250, 454
AluI AGCT 2 cut(s) 250, 454
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 4 cut(s) 87, 195, 419, 434
AoxI GGCC 3 cut(s) 135, 166, 364
ApeKI GCWGC 2 cut(s) 39, 326
ApoI RAATTY 1 cut(s) 413
AspS9I GGNCC 1 cut(s) 167
BanI GGYRCC 1 cut(s) 201
BanII GRGCYC 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 252
BbvI GCAGC 2 cut(s) 51, 313
BceAI ACGGC 3 cut(s) 122, 332, 339
BciT130I CCWGG 1 cut(s) 368
BciVI GTATCC 1 cut(s) 313
BcoDI GTCTC 4 cut(s) 87, 195, 419, 434
BfuI GTATCC 1 cut(s) 313
BisI GCNGC 2 cut(s) 40, 327
BlsI GCNGC 2 cut(s) 41, 328
Bme1390I CCNGG 1 cut(s) 368
BmeRI GACNNNNNGTC 1 cut(s) 187
BmgT120I GGNCC 1 cut(s) 167
BmiI GGNNCC 1 cut(s) 203
BmrFI CCNGG 1 cut(s) 368
BmrI ACTGGG 1 cut(s) 275
BmsI GCATC 1 cut(s) 409
BmuI ACTGGG 1 cut(s) 275
Bsa29I ATCGAT 1 cut(s) 90
BsaJI CCNNGG 1 cut(s) 395
BsaXI ACNNNNNCTCC 2 cut(s) 91, 121
Bse118I RCCGGY 1 cut(s) 133
Bse1I ACTGG 1 cut(s) 281
BseBI CCWGG 1 cut(s) 368
BseCI ATCGAT 1 cut(s) 90
BseDI CCNNGG 1 cut(s) 395
BseGI GGATG 1 cut(s) 391
BseNI ACTGG 1 cut(s) 281
BseRI GAGGAG 2 cut(s) 99, 260
BseX3I CGGCCG 1 cut(s) 135
BseXI GCAGC 2 cut(s) 51, 313
BseYI CCCAGC 1 cut(s) 329
Bsh1285I CGRYCG 2 cut(s) 90, 138
BshFI GGCC 3 cut(s) 137, 168, 366
BshNI GGYRCC 1 cut(s) 201
BshVI ATCGAT 1 cut(s) 90
BsiEI CGRYCG 2 cut(s) 90, 138
BsiHKAI GWGCWC 1 cut(s) 252
BsiSI CCGG 4 cut(s) 116, 134, 165, 338
BsmAI GTCTC 4 cut(s) 87, 195, 419, 434
BsnI GGCC 3 cut(s) 137, 168, 366
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 4 cut(s) 54, 87, 195, 342
BspACI CCGC 3 cut(s) 113, 317, 434
BspANI GGCC 3 cut(s) 137, 168, 366
BspDI ATCGAT 1 cut(s) 90
BspLI GGNNCC 1 cut(s) 203
BspT107I GGYRCC 1 cut(s) 201
BsrFI RCCGGY 1 cut(s) 133
BsrI ACTGG 1 cut(s) 281
BssAI RCCGGY 1 cut(s) 133
BssECI CCNNGG 1 cut(s) 395
BssMI GATC 4 cut(s) 54, 87, 195, 342
Bst2UI CCWGG 1 cut(s) 368
BstC8I GCNNGC 2 cut(s) 135, 376
BstF5I GGATG 1 cut(s) 391
BstKTI GATC 4 cut(s) 57, 90, 198, 345
BstMAI GTCTC 4 cut(s) 87, 195, 419, 434
BstMBI GATC 4 cut(s) 54, 87, 195, 342
BstMCI CGRYCG 2 cut(s) 90, 138
BstMWI GCNNNNNNNGC 2 cut(s) 323, 353
BstNI CCWGG 1 cut(s) 368
BstSCI CCNGG 1 cut(s) 366
BstV1I GCAGC 2 cut(s) 51, 313
BstZI CGGCCG 1 cut(s) 135
Bsu15I ATCGAT 1 cut(s) 90
BsuI GTATCC 1 cut(s) 313
BsuRI GGCC 3 cut(s) 137, 168, 366
BsuTUI ATCGAT 1 cut(s) 90
BtsCI GGATG 1 cut(s) 391
Cac8I GCNNGC 2 cut(s) 135, 376
Cfr10I RCCGGY 1 cut(s) 133
Cfr13I GGNCC 1 cut(s) 167
ClaI ATCGAT 1 cut(s) 90
CviAII CATG 1 cut(s) 437
CviJI RGCY 9 cut(s) 133, 137, 154, 168, 250, 326, 366, 374, 454
CviKI_1 RGCY 9 cut(s) 133, 137, 154, 168, 250, 326, 366, 374, 454
DpnI GATC 4 cut(s) 56, 89, 197, 344
DpnII GATC 4 cut(s) 54, 87, 195, 342
DraIII CACNNNGTG 1 cut(s) 449
DriI GACNNNNNGTC 1 cut(s) 187
EaeI YGGCCR 1 cut(s) 135
EagI CGGCCG 1 cut(s) 135
Eam1105I GACNNNNNGTC 1 cut(s) 187
EciI GGCGGA 2 cut(s) 102, 332
Ecl136II GAGCTC 1 cut(s) 250
EclXI CGGCCG 1 cut(s) 135
Eco24I GRGCYC 1 cut(s) 252
Eco52I CGGCCG 1 cut(s) 135
Eco53kI GAGCTC 1 cut(s) 250
EcoICRI GAGCTC 1 cut(s) 250
EcoRII CCWGG 1 cut(s) 366
EcoT38I GRGCYC 1 cut(s) 252
FaeI CATG 1 cut(s) 440
FaiI YATR 1 cut(s) 438
FatI CATG 1 cut(s) 436
Fnu4HI GCNGC 2 cut(s) 40, 327
FokI GGATG 1 cut(s) 378
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 2 cut(s) 40, 327
GluI GCNGC 2 cut(s) 40, 327
GsaI CCCAGC 1 cut(s) 333
HaeIII GGCC 3 cut(s) 137, 168, 366
HapII CCGG 4 cut(s) 116, 134, 165, 338
Hin1II CATG 1 cut(s) 440
HinfI GANTC 2 cut(s) 188, 287
HpaII CCGG 4 cut(s) 116, 134, 165, 338
Hpy188I TCNGA 3 cut(s) 87, 195, 396
Hpy99I CGWCG 1 cut(s) 188
HpyCH4V TGCA 1 cut(s) 378
HpyF10VI GCNNNNNNNGC 2 cut(s) 323, 353
Hsp92II CATG 1 cut(s) 440
KroI GCCGGC 1 cut(s) 133
KroNI GCCGGC 1 cut(s) 135
Kzo9I GATC 4 cut(s) 54, 87, 195, 342
LmnI GCTCC 1 cut(s) 247
Lsp1109I GCAGC 2 cut(s) 51, 313
LweI GCATC 1 cut(s) 409
MaeIII GTNAC 2 cut(s) 139, 214
MalI GATC 4 cut(s) 56, 89, 197, 344
MboI GATC 4 cut(s) 54, 87, 195, 342
MboII GAAGA 6 cut(s) 64, 133, 136, 185, 239, 313
MhlI GDGCHC 1 cut(s) 252
MluCI AATT 1 cut(s) 413
MlyI GAGTC 2 cut(s) 197, 296
MnlI CCTC 4 cut(s) 120, 217, 238, 390
MroNI GCCGGC 1 cut(s) 133
MseI TTAA 1 cut(s) 411
MspI CCGG 4 cut(s) 116, 134, 165, 338
MspR9I CCNGG 1 cut(s) 368
MvaI CCWGG 1 cut(s) 368
MwoI GCNNNNNNNGC 2 cut(s) 323, 353
NaeI GCCGGC 1 cut(s) 135
NdeII GATC 4 cut(s) 54, 87, 195, 342
NgoMIV GCCGGC 1 cut(s) 133
NlaIII CATG 1 cut(s) 440
NlaIV GGNNCC 1 cut(s) 203
NmeAIII GCCGAG 1 cut(s) 382
NmuCI GTSAC 2 cut(s) 139, 214
PdiI GCCGGC 1 cut(s) 135
PkrI GCNGC 2 cut(s) 41, 328
Ple19I CGATCG 1 cut(s) 90
PleI GAGTC 2 cut(s) 196, 295
PpsI GAGTC 2 cut(s) 196, 295
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 1 cut(s) 366
PspFI CCCAGC 1 cut(s) 329
PspGI CCWGG 1 cut(s) 366
PspN4I GGNNCC 1 cut(s) 203
PspPI GGNCC 1 cut(s) 167
PvuI CGATCG 1 cut(s) 90
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 2 cut(s) 40, 327
Sau3AI GATC 4 cut(s) 54, 87, 195, 342
Sau96I GGNCC 1 cut(s) 167
SchI GAGTC 2 cut(s) 197, 296
ScrFI CCNGG 1 cut(s) 368
SduI GDGCHC 1 cut(s) 252
SetI ASST 3 cut(s) 252, 372, 456
SfaNI GCATC 1 cut(s) 409
Sse9I AATT 1 cut(s) 413
SsiI CCGC 3 cut(s) 113, 317, 434
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 1 cut(s) 366
TaqI TCGA 4 cut(s) 90, 252, 276, 290
TasI AATT 1 cut(s) 413
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TseFI GTSAC 2 cut(s) 139, 214
TseI GCWGC 2 cut(s) 39, 326
Tsp45I GTSAC 2 cut(s) 139, 214
TspDTI ATGAA 3 cut(s) 199, 253, 314
XapI RAATTY 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.