Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0004521

Physical Location & Seq

Genomic Coordinates Forward (+)

931665 .. 933358

Gene Structure 1694 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1014200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1593 bp

ATGGGTTCGGCTGGCTCGAAGAGCAAGCAAGACAATGCGAAGAGAAAGGACTTGATGAAATCAGTGAAGATGAACAGGTACGCGTTTGCGGATGCACAGCGCGAGCATATTCTCGCGCTCCTTAAAGTAGCGGAGGCTATAAAGTTGTTTGTAGAAAGCCATTCTTTCGAAGTTTCGACGCACTCTTCAAATGCAGACAGAGGAAACGAGCTGCAGGAAAACTCAGACTGTGCTGAGACAGATGAGGAACAAGAGGAGGTGGATCAAACAGTGCCGTCACCGCGGGGAGATTCCGATTGCTCCGATGGTGACGGTGATAGAAAAGAGGTGGTGAAGACAAGTAATGAAAAGGGTAGAGAGAGAGAATTGTTGGAAGCATTGAAAGATGTCGAAGACTACTTCCGTCGGGCGTATGATTCTGGTTTGCACGTTTCGAGGATGTTAGAGACCGACATGGCTCAGGTGCAATCAGCAGCCGAGGAAATTAAAGGGAATCTCAACCGTTCAATGACGCGGAGCCGCTCCATCTCATCTACATTATCTTGGTCTTCCTCTCGTAAAAGTCTACGTAAATCTAGCTTAAGAAGTTCTTCAACACGGGCTTGTTTCGATGGTGGTCTCCTCGGTGACAAGGGATCTCCAAGCCATTCGTTAACGATAGGAAAGCTATATGCTTTGGAGGAAAACCTCTACAGCGAGCAGAAGGTAATAGAGGAAACTGAGAAAATTTGTGACCGAAAATCGGAAGATCTAGACACCAAAATATACGAGATTGAAAGCTTGTATTGCAGAATTTTGACGGCCAAAAAGAATGCTAAATCAATCTCCGAGACAATTCGGAAAATGAGAGATGAAGAGCTACAACCACAGCTCGTTAAGTTGTTAAAAAGCATAATGAGCAACTCAAAGAAAATGTCTGAATTTCTGGAAACCCAAAAGAGAATAATGTCCGAAGTGAACTCTCTCAAATGTCCGTCCAATGGAACGCTCTGCAATGACTCGAACCAGGTTGCTACTCGGATGCTCGAAAAGGAACTTCTAAATTGGCGTACACACTTTGCGTCGTATGTTTCTTCGCAAAAGGCGTACGTCAAAGCTCTAGACCGCTGGCTAGCTAAGTTGGACGATGCTGAAACTGAAAGCAAGTTAAAATCAAAGGGGGTTAACCCGTCATCGCTTGCAATTTGTCGACAGTGGTCAGATGGCCTAGATAAGCTGCCGTATCAAGCTGTGACTGGCGCCATCAAAAGCTTCGTGAAAGATGTCCGGGCACTGAGGGATCAACAACATAAGGAGCGCCGACAAAAGAGGGAGGTTGACAAGCTTGCCAGAAGACTCAGGAGGGAGAATTTTTTGCAGGAAACAGACCTCAAGGAACAGATGCGGACTGAGAAGAAGAAACTTCATACTATTACGCAGAAGATGCAAGATATCGCTGTGAACGGATTTCAGACGCAGTTTTCTGAAGTTTTCGGTTCCATGGCTAAATTTTTCGACTCCATGGCCGAGTTGTTTTCAAAGTCTGCTACAAAAAAGTCTGGTCGTCCAAGCAAAAATGCAAGCAGGACTCGTGTGAAAGCGATCGACAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

531

Amino Acids

60.28

Weight (kDa)

9.16

Isoelectric Point (pI)

51.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF630	PF04783	1 - 54	6.6e-06	Protein of unknown function (DUF630)
DUF632	PF04782	123 - 423	2.2e-61	Protein of unknown function (DUF632)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004521)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g11140 FvH4_3g42260
malus_domestica	MD02G1014200.v1.1
prunus_persica	Prupe.7G172100_v2.0.a1 Prupe.7G174400_v2.0.a1 Prupe.7G174500_v2.0.a1
pyrus_communis	pycom02g01300
rosa_chinensis	RchiOBHm_Chr2g0098421
rosa_laevigata	RLG00000016798 RLG00000036577 RLG00000036578 RLG00000036581
rosa_multiflora	Rmu_sc0003437.1_g000007 Rmu_ssc0000196.1_g000020
rosa_roxburghii	Rroxscaffold_1G00005980
rosa_rugosa	Rorug02G0073100 Rorug05G0440800.1 Rorug05G0441000 Rorug05G0441100
rosa_samantha	Rh2AG120400 Rh2BG123200 Rh2CG124800 Rh2DG124900 Rh5AG497900 Rh5BG518700 Rh5BG518800 Rh5CG542400 Rh5CG542500 Rh5DG530200 Rh5DG530400
rosa_wichuraiana	Rw2G009420 Rw5G046150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1238
AccBSI	CCGCTC	1 cut(s)	522
AccI	GTMKAC	2 cut(s)	565, 1189
AccII	CGCG	5 cut(s)	83, 102, 116, 283, 514
AciI	CCGC	8 cut(s)	89, 131, 281, 283, 514, 520, 1105, 1384
AclWI	GGATC	3 cut(s)	270, 643, 1287
AcoI	YGGCCR	2 cut(s)	801, 1503
AcsI	RAATTY	5 cut(s)	726, 792, 920, 1348, 1487
AcuI	CTGAAG	1 cut(s)	1485
AcyI	GRCGYC	1 cut(s)	1239
AfaI	GTAC	3 cut(s)	80, 1051, 1088
AfiI	CCNNNNNNNGG	2 cut(s)	742, 980
AflII	CTTAAG	1 cut(s)	580
AflIII	ACRYGT	1 cut(s)	81
AgsI	TTSAA	6 cut(s)	189, 382, 507, 594, 776, 1518
AjnI	CCWGG	1 cut(s)	1005
Alw26I	GTCTC	4 cut(s)	230, 440, 623, 824
AlwI	GGATC	3 cut(s)	270, 643, 1287
AoxI	GGCC	3 cut(s)	801, 1204, 1503
ApeKI	GCWGC	3 cut(s)	211, 473, 1216
ApoI	RAATTY	5 cut(s)	726, 792, 920, 1348, 1487
ArsI	GACNNNNNNTTYG	2 cut(s)	899, 931
Asp700I	GAANNNNTTC	1 cut(s)	589
AspLEI	GCGC	4 cut(s)	102, 118, 1241, 1299
AsuC2I	CCSGG	1 cut(s)	1268
AsuHPI	GGTGA	5 cut(s)	270, 320, 326, 343, 638
AsuII	TTCGAA	1 cut(s)	168
AsuNHI	GCTAGC	1 cut(s)	1111
BaeGI	GKGCMC	1 cut(s)	1273
BanI	GGYRCC	1 cut(s)	1238
BauI	CACGAG	1 cut(s)	1569
BbsI	GAAGAC	4 cut(s)	341, 399, 540, 1339
BbvI	GCAGC	3 cut(s)	198, 485, 1203
BccI	CCATC	5 cut(s)	299, 533, 605, 1196, 1250
BceAI	ACGGC	3 cut(s)	259, 816, 1204
BciT130I	CCWGG	1 cut(s)	1007
BcnI	CCSGG	1 cut(s)	1268
BcoDI	GTCTC	4 cut(s)	230, 440, 623, 824
BfaI	CTAG	5 cut(s)	576, 752, 1100, 1112, 1208
BfmI	CTRYAG	2 cut(s)	212, 691
BfoI	RGCGCY	2 cut(s)	1242, 1300
BfrI	CTTAAG	1 cut(s)	580
BglII	AGATCT	1 cut(s)	748
BisI	GCNGC	4 cut(s)	212, 474, 520, 1217
BlsI	GCNGC	4 cut(s)	213, 475, 521, 1218
Bme1390I	CCNGG	2 cut(s)	1007, 1268
BmiI	GGNNCC	3 cut(s)	518, 1240, 1477
BmrFI	CCNGG	2 cut(s)	1007, 1268
BmsI	GCATC	5 cut(s)	82, 1011, 1117, 1371, 1413
BmtI	GCTAGC	1 cut(s)	1115
BoxI	GACNNNNGTC	1 cut(s)	1195
BpiI	GAAGAC	4 cut(s)	341, 399, 540, 1339
Bpu10I	CCTNAGC	1 cut(s)	459
Bpu14I	TTCGAA	1 cut(s)	168
BpuEI	CTTGAG	1 cut(s)	1355
BpuMI	CCSGG	1 cut(s)	1268
BsaAI	YACGTR	1 cut(s)	569
BsaHI	GRCGYC	1 cut(s)	1239
BsaI	GGTCTC	2 cut(s)	440, 623
BsaJI	CCNNGG	5 cut(s)	281, 477, 622, 1479, 1500
Bsc4I	CCNNNNNNNGG	2 cut(s)	742, 980
Bse1I	ACTGG	1 cut(s)	1240
Bse3DI	GCAATG	1 cut(s)	1000
BseBI	CCWGG	1 cut(s)	1007
BseDI	CCNNGG	5 cut(s)	281, 477, 622, 1479, 1500
BseGI	GGATG	3 cut(s)	97, 444, 1026
BseLI	CCNNNNNNNGG	2 cut(s)	742, 980
BseMI	GCAATG	1 cut(s)	1000
BseMII	CTCAG	7 cut(s)	225, 237, 473, 711, 1265, 1351, 1380
BseNI	ACTGG	1 cut(s)	1240
BseRI	GAGGAG	2 cut(s)	269, 611
BseSI	GKGCMC	1 cut(s)	1273
BseXI	GCAGC	3 cut(s)	198, 485, 1203
Bsh1236I	CGCG	5 cut(s)	83, 102, 116, 283, 514
Bsh1285I	CGRYCG	1 cut(s)	1584
BshFI	GGCC	3 cut(s)	803, 1206, 1505
BshNI	GGYRCC	1 cut(s)	1238
BsiEI	CGRYCG	1 cut(s)	1584
BsiSI	CCGG	1 cut(s)	1267
BsiWI	CGTACG	1 cut(s)	1086
BslI	CCNNNNNNNGG	2 cut(s)	742, 980
BsmAI	GTCTC	4 cut(s)	230, 440, 623, 824
BsmI	GAATGC	1 cut(s)	817
BsnI	GGCC	3 cut(s)	803, 1206, 1505
Bso31I	GGTCTC	2 cut(s)	440, 623
Bsp119I	TTCGAA	1 cut(s)	168
Bsp1286I	GDGCHC	1 cut(s)	1273
Bsp143I	GATC	5 cut(s)	262, 635, 748, 1279, 1581
Bsp19I	CCATGG	2 cut(s)	1479, 1500
BspACI	CCGC	8 cut(s)	89, 131, 281, 283, 514, 520, 1105, 1384
BspANI	GGCC	3 cut(s)	803, 1206, 1505
BspCNI	CTCAG	7 cut(s)	226, 236, 472, 712, 1266, 1350, 1381
BspFNI	CGCG	5 cut(s)	83, 102, 116, 283, 514
BspLI	GGNNCC	3 cut(s)	518, 1240, 1477
BspMAI	CTGCAG	1 cut(s)	216
BspOI	GCTAGC	1 cut(s)	1115
BspPI	GGATC	3 cut(s)	270, 643, 1287
BspQI	GCTCTTC	2 cut(s)	14, 849
BspT104I	TTCGAA	1 cut(s)	168
BspT107I	GGYRCC	1 cut(s)	1238
BspTI	CTTAAG	1 cut(s)	580
BspTNI	GGTCTC	2 cut(s)	440, 623
BsrBI	CCGCTC	1 cut(s)	522
BsrDI	GCAATG	1 cut(s)	1000
BsrI	ACTGG	1 cut(s)	1240
BssECI	CCNNGG	5 cut(s)	281, 477, 622, 1479, 1500
BssMI	GATC	5 cut(s)	262, 635, 748, 1279, 1581
BssNI	GRCGYC	1 cut(s)	1239
BssSI	CACGAG	1 cut(s)	1569
BssT1I	CCWWGG	2 cut(s)	1479, 1500
Bst2BI	CACGAG	1 cut(s)	1569
Bst2UI	CCWGG	1 cut(s)	1007
Bst4CI	ACNGT	5 cut(s)	230, 271, 314, 503, 1194
Bst6I	CTCTTC	4 cut(s)	14, 35, 190, 849
BstACI	GRCGYC	1 cut(s)	1239
BstAFI	CTTAAG	1 cut(s)	580
BstAPI	GCANNNNNTGC	1 cut(s)	1423
BstBAI	YACGTR	1 cut(s)	569
BstBI	TTCGAA	1 cut(s)	168
BstC8I	GCNNGC	9 cut(s)	13, 26, 104, 698, 1109, 1113, 1179, 1326, 1561
BstDEI	CTNAG	8 cut(s)	223, 234, 459, 720, 1116, 1274, 1337, 1389
BstDSI	CCRYGG	3 cut(s)	281, 1479, 1500
BstF5I	GGATG	3 cut(s)	97, 444, 1026
BstFNI	CGCG	5 cut(s)	83, 102, 116, 283, 514
BstH2I	RGCGCY	2 cut(s)	1242, 1300
BstHHI	GCGC	4 cut(s)	102, 118, 1241, 1299
BstKTI	GATC	5 cut(s)	265, 638, 751, 1282, 1584
BstMAI	GTCTC	4 cut(s)	230, 440, 623, 824
BstMBI	GATC	5 cut(s)	262, 635, 748, 1279, 1581
BstMCI	CGRYCG	1 cut(s)	1584
BstMWI	GCNNNNNNNGC	5 cut(s)	21, 280, 786, 897, 1423
BstNI	CCWGG	1 cut(s)	1007
BstPAI	GACNNNNGTC	1 cut(s)	1195
BstSCI	CCNGG	2 cut(s)	1005, 1266
BstSFI	CTRYAG	2 cut(s)	212, 691
BstSLI	GKGCMC	1 cut(s)	1273
BstSNI	TACGTA	1 cut(s)	569
BstUI	CGCG	5 cut(s)	83, 102, 116, 283, 514
BstV1I	GCAGC	3 cut(s)	198, 485, 1203
BstV2I	GAAGAC	4 cut(s)	341, 399, 540, 1339
BstX2I	RGATCY	2 cut(s)	635, 748
BstYI	RGATCY	2 cut(s)	635, 748
BsuRI	GGCC	3 cut(s)	803, 1206, 1505
BtgI	CCRYGG	3 cut(s)	281, 1479, 1500
BtgZI	GCGATG	1 cut(s)	1158
BtsCI	GGATG	3 cut(s)	97, 444, 1026
BtsIMutI	CAGTG	4 cut(s)	69, 276, 1199, 1271
Cac8I	GCNNGC	9 cut(s)	13, 26, 104, 698, 1109, 1113, 1179, 1326, 1561
CfoI	GCGC	4 cut(s)	102, 118, 1241, 1299
Cfr42I	CCGCGG	1 cut(s)	284
CseI	GACGC	4 cut(s)	187, 520, 1050, 1462
CsiI	ACCWGGT	1 cut(s)	1005
Csp6I	GTAC	3 cut(s)	79, 1050, 1087
CviAII	CATG	3 cut(s)	454, 1480, 1501
CviQI	GTAC	3 cut(s)	79, 1050, 1087
DdeI	CTNAG	8 cut(s)	223, 234, 459, 720, 1116, 1274, 1337, 1389
DinI	GGCGCC	1 cut(s)	1240
DpnI	GATC	5 cut(s)	264, 637, 750, 1281, 1583
DpnII	GATC	5 cut(s)	262, 635, 748, 1279, 1581
EaeI	YGGCCR	2 cut(s)	801, 1503
Eam1104I	CTCTTC	4 cut(s)	14, 35, 190, 849
EarI	CTCTTC	4 cut(s)	14, 35, 190, 849
Eco105I	TACGTA	1 cut(s)	569
Eco130I	CCWWGG	2 cut(s)	1479, 1500
Eco31I	GGTCTC	2 cut(s)	440, 623
Eco32I	GATATC	1 cut(s)	1432
Eco57I	CTGAAG	1 cut(s)	1485
EcoRII	CCWGG	1 cut(s)	1005
EcoRV	GATATC	1 cut(s)	1432
EcoT14I	CCWWGG	2 cut(s)	1479, 1500
EgeI	GGCGCC	1 cut(s)	1240
EheI	GGCGCC	1 cut(s)	1240
ErhI	CCWWGG	2 cut(s)	1479, 1500
FaeI	CATG	3 cut(s)	457, 1483, 1504
FalI	AAGNNNNNCTT	4 cut(s)	148, 180, 574, 606
FatI	CATG	3 cut(s)	453, 1479, 1500
FauI	CCCGC	1 cut(s)	276
FblI	GTMKAC	2 cut(s)	565, 1189
Fnu4HI	GCNGC	4 cut(s)	212, 474, 520, 1217
FokI	GGATG	3 cut(s)	104, 451, 1033
Fsp4HI	GCNGC	4 cut(s)	212, 474, 520, 1217
FspBI	CTAG	5 cut(s)	576, 752, 1100, 1112, 1208
GlaI	GCGC	4 cut(s)	101, 117, 1240, 1298
GluI	GCNGC	4 cut(s)	212, 474, 520, 1217
HaeII	RGCGCY	2 cut(s)	1242, 1300
HaeIII	GGCC	3 cut(s)	803, 1206, 1505
HapII	CCGG	1 cut(s)	1267
HgaI	GACGC	4 cut(s)	187, 520, 1050, 1462
HhaI	GCGC	4 cut(s)	102, 118, 1241, 1299
Hin1I	GRCGYC	1 cut(s)	1239
Hin1II	CATG	3 cut(s)	457, 1483, 1504
Hin6I	GCGC	4 cut(s)	100, 116, 1239, 1297
HinP1I	GCGC	4 cut(s)	100, 116, 1239, 1297
HincII	GTYRAC	4 cut(s)	654, 1165, 1190, 1318
HindII	GTYRAC	4 cut(s)	654, 1165, 1190, 1318
HindIII	AAGCTT	3 cut(s)	778, 1249, 1322
HinfI	GANTC	7 cut(s)	290, 416, 493, 998, 1335, 1496, 1567
HpaI	GTTAAC	2 cut(s)	654, 1165
HpaII	CCGG	1 cut(s)	1267
HphI	GGTGA	5 cut(s)	270, 320, 326, 343, 638
Hpy166II	GTNNAC	8 cut(s)	566, 654, 958, 1052, 1165, 1190, 1318, 1441
Hpy188III	TCNNGA	5 cut(s)	752, 926, 1100, 1255, 1339
Hpy8I	GTNNAC	8 cut(s)	566, 654, 958, 1052, 1165, 1190, 1318, 1441
Hpy99I	CGWCG	3 cut(s)	181, 408, 1066
HpyAV	CCTTC	1 cut(s)	697
HpyCH4III	ACNGT	5 cut(s)	230, 271, 314, 503, 1194
HpyCH4IV	ACGT	3 cut(s)	429, 568, 1089
HpyF10VI	GCNNNNNNNGC	5 cut(s)	21, 280, 786, 897, 1423
HpyF3I	CTNAG	8 cut(s)	223, 234, 459, 720, 1116, 1274, 1337, 1389
HpySE526I	ACGT	3 cut(s)	429, 568, 1089
Hsp92I	GRCGYC	1 cut(s)	1239
Hsp92II	CATG	3 cut(s)	457, 1483, 1504
HspAI	GCGC	4 cut(s)	100, 116, 1239, 1297
KasI	GGCGCC	1 cut(s)	1238
KspAI	GTTAAC	2 cut(s)	654, 1165
KspI	CCGCGG	1 cut(s)	284
Kzo9I	GATC	5 cut(s)	262, 635, 748, 1279, 1581
LguI	GCTCTTC	2 cut(s)	14, 849
LmnI	GCTCC	5 cut(s)	123, 305, 516, 527, 1294
Lsp1109I	GCAGC	3 cut(s)	198, 485, 1203
LweI	GCATC	5 cut(s)	82, 1011, 1117, 1371, 1413
MabI	ACCWGGT	1 cut(s)	1005
MaeI	CTAG	5 cut(s)	576, 752, 1100, 1112, 1208
MaeII	ACGT	3 cut(s)	429, 568, 1089
MaeIII	GTNAC	5 cut(s)	276, 308, 626, 731, 1231
MalI	GATC	5 cut(s)	264, 637, 750, 1281, 1583
MbiI	CCGCTC	1 cut(s)	522
MboI	GATC	5 cut(s)	262, 635, 748, 1279, 1581
MflI	RGATCY	2 cut(s)	635, 748
MhlI	GDGCHC	1 cut(s)	1273
MluI	ACGCGT	1 cut(s)	81
Mly113I	GGCGCC	1 cut(s)	1239
MlyI	GAGTC	4 cut(s)	992, 1329, 1490, 1561
MmeI	TCCRAC	2 cut(s)	351, 1101
MroXI	GAANNNNTTC	1 cut(s)	589
MseI	TTAA	8 cut(s)	123, 486, 581, 653, 876, 884, 1148, 1164
MspA1I	CMGCKG	2 cut(s)	283, 1107
MspCI	CTTAAG	1 cut(s)	580
MspI	CCGG	1 cut(s)	1267
MspR9I	CCNGG	2 cut(s)	1007, 1268
Mva1269I	GAATGC	1 cut(s)	817
MvaI	CCWGG	1 cut(s)	1007
MvnI	CGCG	5 cut(s)	83, 102, 116, 283, 514
MwoI	GCNNNNNNNGC	5 cut(s)	21, 280, 786, 897, 1423
NarI	GGCGCC	1 cut(s)	1239
NciI	CCSGG	1 cut(s)	1268
NcoI	CCATGG	2 cut(s)	1479, 1500
NdeII	GATC	5 cut(s)	262, 635, 748, 1279, 1581
NheI	GCTAGC	1 cut(s)	1111
NlaIII	CATG	3 cut(s)	457, 1483, 1504
NlaIV	GGNNCC	3 cut(s)	518, 1240, 1477
NmeAIII	GCCGAG	2 cut(s)	502, 1531
NmuCI	GTSAC	5 cut(s)	276, 308, 626, 731, 1231
NspV	TTCGAA	1 cut(s)	168
PciSI	GCTCTTC	2 cut(s)	14, 849
PcsI	WCGNNNNNNNCGW	2 cut(s)	14, 1082
PctI	GAATGC	1 cut(s)	817
PdmI	GAANNNNTTC	1 cut(s)	589
PfeI	GAWTC	3 cut(s)	290, 416, 493
Pfl23II	CGTACG	1 cut(s)	1086
PkrI	GCNGC	4 cut(s)	213, 475, 521, 1218
Ple19I	CGATCG	1 cut(s)	1584
PleI	GAGTC	4 cut(s)	992, 1329, 1490, 1561
PluTI	GGCGCC	1 cut(s)	1242
PpsI	GAGTC	4 cut(s)	992, 1329, 1490, 1561
Ppu21I	YACGTR	1 cut(s)	569
PshAI	GACNNNNGTC	1 cut(s)	1195
Psp6I	CCWGG	1 cut(s)	1005
PspGI	CCWGG	1 cut(s)	1005
PspLI	CGTACG	1 cut(s)	1086
PspN4I	GGNNCC	3 cut(s)	518, 1240, 1477
PstI	CTGCAG	1 cut(s)	216
PsuI	RGATCY	2 cut(s)	635, 748
PvuI	CGATCG	1 cut(s)	1584
RsaI	GTAC	3 cut(s)	80, 1051, 1088
RsaNI	GTAC	3 cut(s)	79, 1050, 1087
SacII	CCGCGG	1 cut(s)	284
SalI	GTCGAC	1 cut(s)	1188
SapI	GCTCTTC	2 cut(s)	14, 849
SaqAI	TTAA	8 cut(s)	123, 486, 581, 653, 876, 884, 1148, 1164
SatI	GCNGC	4 cut(s)	212, 474, 520, 1217
Sau3AI	GATC	5 cut(s)	262, 635, 748, 1279, 1581
SchI	GAGTC	4 cut(s)	992, 1329, 1490, 1561
ScrFI	CCNGG	2 cut(s)	1007, 1268
SduI	GDGCHC	1 cut(s)	1273
SexAI	ACCWGGT	1 cut(s)	1005
SfaNI	GCATC	5 cut(s)	82, 1011, 1117, 1371, 1413
SfcI	CTRYAG	2 cut(s)	212, 691
SfoI	GGCGCC	1 cut(s)	1240
Sfr303I	CCGCGG	1 cut(s)	284
SfuI	TTCGAA	1 cut(s)	168
SgrBI	CCGCGG	1 cut(s)	284
SmlI	CTYRAG	2 cut(s)	580, 1370
SmoI	CTYRAG	2 cut(s)	580, 1370
SnaBI	TACGTA	1 cut(s)	569
SsiI	CCGC	8 cut(s)	89, 131, 281, 283, 514, 520, 1105, 1384
SspDI	GGCGCC	1 cut(s)	1238
SspMI	CTAG	5 cut(s)	576, 752, 1100, 1112, 1208
StyD4I	CCNGG	2 cut(s)	1005, 1266
StyI	CCWWGG	2 cut(s)	1479, 1500
TaaI	ACNGT	5 cut(s)	230, 271, 314, 503, 1194
TaiI	ACGT	3 cut(s)	432, 571, 1092
TaqII	GACCGA	2 cut(s)	464, 750
TauI	GCSGC	1 cut(s)	522
TfiI	GAWTC	3 cut(s)	290, 416, 493
Tru1I	TTAA	8 cut(s)	123, 486, 581, 653, 876, 884, 1148, 1164
Tru9I	TTAA	8 cut(s)	123, 486, 581, 653, 876, 884, 1148, 1164
TscAI	CASTG	4 cut(s)	69, 276, 1199, 1278
TseFI	GTSAC	5 cut(s)	276, 308, 626, 731, 1231
TseI	GCWGC	3 cut(s)	211, 473, 1216
Tsp45I	GTSAC	5 cut(s)	276, 308, 626, 731, 1231
TspDTI	ATGAA	5 cut(s)	71, 86, 360, 867, 1394
TspGWI	ACGGA	3 cut(s)	392, 963, 1458
TspRI	CASTG	4 cut(s)	69, 276, 1199, 1278
Vha464I	CTTAAG	1 cut(s)	580
XapI	RAATTY	5 cut(s)	726, 792, 920, 1348, 1487
XbaI	TCTAGA	2 cut(s)	751, 1099
XmiI	GTMKAC	2 cut(s)	565, 1189
XmnI	GAANNNNTTC	1 cut(s)	589
XspI	CTAG	5 cut(s)	576, 752, 1100, 1112, 1208

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.