Rh5DG530400

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004521
Physical Location & Seq
Forward (+)
81859225 .. 81860655
1431 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG530400.1

Sequence Viewer

Length: 1431 bp
ATGGAGTCCAATCATAAATTTCTAGCCAAAAAAATATCTCTTTGTAAAAAGAGACGGAAGGATCTGACAAAGGCTTTAGAGCGAAGGTGTGCCCTTATGAAACAACAGCTGAAGTTTAATCGCTGTCACCAAGAAGTAGCCGATGCCATAATGGCGTTCATCAAGGAACAGAATCCCTCTCCCTCTGAAATCCCGGAAGCTAGAGGCGAACTAGTTGAATTTTCGATTATGGAGAATGCTGATGAGGGTGGTTTGATCACAAGGAGACAAGGGAATAATGATGTGCCAGAAAGGAGGGACGTCTCGCAATTCTTGAAGGCATTGGAAGAGGTCAAAGAACTTTTTCGTAACTCCCATGAGTCTGGTAAGGATGTCTCAAAGAAGTTGGAGACAGACAGTATTGGAATCAAAGCTGAATCATCACTACCTTCGAAACTGGTCAGGTCACTGTCAAGAAGCAGCTCTAGAGTTGGTTGTTCAACCTTTTATGGTTCTGAAATAATGGGTGAAACCCTAGGAGCACTGAGGCGTTGGGAGGAGAAACTCTATCATGAACTGAAGACGATCAAAAAGGCCGAACCCAAGAGGCAGAAACCCGAAGATTCCAGCAGCGAAAACCATCCTAATTCAGAAACCGAGATGAAAATAATGTCGAATGTGAAATGTAAAGTTGCCAAAAGGGCCGCTGATAATGACTCCGAAGAGATTACAAGATTGAGAAACGAATGTTTGCTTCCCAAACTTGGTGAGCAGCTAGATGGGTTGGCGGGAAACTGGAAAGCAATGTCAGAGTTTCATGAAAGCCAAAAGAAGATTATGTCTCAAGTAAGAGAATCTCCCAACCATCCATTACTCTGCGAAGATGAGCACCAAAAAGCTACCAAAAAGCTCAAAGCACAGCTTTGCAATTGGCTCGACTGCTTTAAGTCCTACGTTTCTCTACAAAAGAAATACATGGAATCTCTTTGTTGGTGGCTCTTTCATAACGACAAACCAATAATACTCGGAGACAATGATGATGATTGGTTGATTAACCTGAAAAATCTGGAAAGTAATACAAAGGAGGTGACTACTGAGATGAAAAGGTTCCAGAAAGATGTTGCAGCCCTATTAGGCCAACAAGGGAAGGAGCATGACCAAAAGAGGGAGGTCAAGCGACTTGAGAGCCGACTCGAGATTAAGAAGCGGATGATTAATCCTGTGAACGAGAAGCAAACCTTGGAGGAGATTGAGAAGTTAAAGGAGAGGATCGAGAGAACTAAAGAGACGCATCAAAGTAAAATGCAGGAGACGCAGCAAATGGTGAATCAATTTCAGACAGGGTTCTCTGATGTTTTCCAGAAGCTAGCGGATTTTTCAAGAAAAGCCAACGAAATCTATACTGAGCTTATCAAACACCTGCCAAAAACCCATCTGGATACGCAAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

476

Amino Acids

55.32

Weight (kDa)

8.83

Isoelectric Point (pI)

58.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 105 - 329 2e-30 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1407
AatII GACGTC 1 cut(s) 303
Acc36I ACCTGC 1 cut(s) 1407
AciI CCGC 4 cut(s) 684, 767, 1186, 1349
AclWI GGATC 2 cut(s) 69, 1256
AcsI RAATTY 2 cut(s) 17, 218
AcuI CTGAAG 2 cut(s) 131, 578
AcyI GRCGYC 1 cut(s) 300
AfiI CCNNNNNNNGG 2 cut(s) 743, 1144
AgsI TTSAA 4 cut(s) 218, 316, 480, 1359
AhlI ACTAGT 1 cut(s) 211
AjuI GAANNNNNNNTTGG 2 cut(s) 1202, 1234
Alw21I GWGCWC 2 cut(s) 523, 870
Alw26I GTCTC 9 cut(s) 46, 259, 307, 379, 383, 825, 1002, 1259, 1283
AlwI GGATC 2 cut(s) 69, 1256
Ama87I CYCGRG 1 cut(s) 1172
AoxI GGCC 3 cut(s) 573, 681, 1114
ApeKI GCWGC 5 cut(s) 459, 609, 751, 1103, 1294
ApoI RAATTY 2 cut(s) 17, 218
AseI ATTAAT 1 cut(s) 1194
Asp700I GAANNNNTTC 2 cut(s) 342, 1085
AspA2I CCTAGG 1 cut(s) 514
AspS9I GGNCC 1 cut(s) 681
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 5 cut(s) 119, 518, 758, 1078, 1315
AsuII TTCGAA 1 cut(s) 431
AsuNHI GCTAGC 1 cut(s) 1345
AvaI CYCGRG 1 cut(s) 1172
AvrII CCTAGG 1 cut(s) 514
BaeGI GKGCMC 1 cut(s) 94
BbsI GAAGAC 1 cut(s) 566
Bbv12I GWGCWC 2 cut(s) 523, 870
BbvI GCAGC 5 cut(s) 471, 621, 763, 1115, 1306
BccI CCATC 4 cut(s) 627, 752, 852, 1419
BcgI CGANNNNNNTGC 2 cut(s) 895, 929
BciVI GTATCC 1 cut(s) 1411
BclI TGATCA 1 cut(s) 255
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 9 cut(s) 46, 259, 307, 379, 383, 825, 1002, 1259, 1283
BcuI ACTAGT 1 cut(s) 211
BfaI CTAG 7 cut(s) 23, 201, 212, 465, 515, 755, 1346
BfuAI ACCTGC 1 cut(s) 1407
BfuI GTATCC 1 cut(s) 1411
BglI GCCNNNNNGGC 2 cut(s) 152, 680
BisI GCNGC 6 cut(s) 460, 610, 684, 752, 1104, 1295
BlnI CCTAGG 1 cut(s) 514
BlsI GCNGC 6 cut(s) 461, 611, 685, 753, 1105, 1296
Bme1390I CCNGG 1 cut(s) 194
BmeT110I CYCGRG 1 cut(s) 1172
BmgT120I GGNCC 1 cut(s) 681
BmiI GGNNCC 1 cut(s) 1088
BmrFI CCNGG 1 cut(s) 194
BmsI GCATC 2 cut(s) 133, 1279
BmtI GCTAGC 1 cut(s) 1349
BpiI GAAGAC 1 cut(s) 566
Bpu14I TTCGAA 1 cut(s) 431
BpuEI CTTGAG 2 cut(s) 807, 1181
BpuMI CCSGG 1 cut(s) 194
BsaHI GRCGYC 1 cut(s) 300
BsaJI CCNNGG 2 cut(s) 514, 1218
Bsc4I CCNNNNNNNGG 2 cut(s) 743, 1144
Bse1I ACTGG 2 cut(s) 441, 779
Bse3DI GCAATG 1 cut(s) 789
BseDI CCNNGG 2 cut(s) 514, 1218
BseGI GGATG 4 cut(s) 376, 619, 844, 1194
BseLI CCNNNNNNNGG 2 cut(s) 743, 1144
BseMI GCAATG 1 cut(s) 789
BseMII CTCAG 3 cut(s) 515, 1065, 1374
BseNI ACTGG 2 cut(s) 441, 779
BseRI GAGGAG 2 cut(s) 551, 1238
BseSI GKGCMC 1 cut(s) 94
BseXI GCAGC 5 cut(s) 471, 621, 763, 1115, 1306
BshFI GGCC 3 cut(s) 575, 683, 1116
BsiHKAI GWGCWC 2 cut(s) 523, 870
BsiHKCI CYCGRG 1 cut(s) 1172
BsiSI CCGG 1 cut(s) 194
BslFI GGGAC 1 cut(s) 311
BslI CCNNNNNNNGG 2 cut(s) 743, 1144
BsmAI GTCTC 9 cut(s) 46, 259, 307, 379, 383, 825, 1002, 1259, 1283
BsmBI CGTCTC 4 cut(s) 46, 307, 1259, 1283
BsmFI GGGAC 1 cut(s) 311
BsmI GAATGC 1 cut(s) 241
BsnI GGCC 3 cut(s) 575, 683, 1116
BsoBI CYCGRG 1 cut(s) 1172
Bsp119I TTCGAA 1 cut(s) 431
Bsp1286I GDGCHC 3 cut(s) 94, 523, 870
Bsp143I GATC 4 cut(s) 61, 255, 564, 1248
BspACI CCGC 4 cut(s) 684, 767, 1186, 1349
BspANI GGCC 3 cut(s) 575, 683, 1116
BspCNI CTCAG 3 cut(s) 516, 1066, 1375
BspHI TCATGA 2 cut(s) 550, 796
BspLI GGNNCC 1 cut(s) 1088
BspMI ACCTGC 1 cut(s) 1407
BspOI GCTAGC 1 cut(s) 1349
BspPI GGATC 2 cut(s) 69, 1256
BspT104I TTCGAA 1 cut(s) 431
BsrDI GCAATG 1 cut(s) 789
BsrI ACTGG 2 cut(s) 441, 779
BssECI CCNNGG 2 cut(s) 514, 1218
BssMI GATC 4 cut(s) 61, 255, 564, 1248
BssNI GRCGYC 1 cut(s) 300
BssT1I CCWWGG 2 cut(s) 514, 1218
Bst4CI ACNGT 2 cut(s) 398, 450
Bst6I CTCTTC 2 cut(s) 321, 696
BstACI GRCGYC 1 cut(s) 300
BstBI TTCGAA 1 cut(s) 431
BstC8I GCNNGC 1 cut(s) 1347
BstDEI CTNAG 3 cut(s) 524, 1074, 1383
BstF5I GGATG 4 cut(s) 376, 619, 844, 1194
BstKTI GATC 4 cut(s) 64, 258, 567, 1251
BstMAI GTCTC 9 cut(s) 46, 259, 307, 379, 383, 825, 1002, 1259, 1283
BstMBI GATC 4 cut(s) 61, 255, 564, 1248
BstMWI GCNNNNNNNGC 3 cut(s) 152, 680, 1291
BstSCI CCNGG 1 cut(s) 192
BstSLI GKGCMC 1 cut(s) 94
BstV1I GCAGC 5 cut(s) 471, 621, 763, 1115, 1306
BstV2I GAAGAC 1 cut(s) 566
BstX2I RGATCY 1 cut(s) 61
BstXI CCANNNNNNTGG 1 cut(s) 362
BstYI RGATCY 1 cut(s) 61
BsuI GTATCC 1 cut(s) 1411
BsuRI GGCC 3 cut(s) 575, 683, 1116
BtsCI GGATG 4 cut(s) 376, 619, 844, 1194
BtsIMutI CAGTG 2 cut(s) 446, 521
BveI ACCTGC 1 cut(s) 1407
Cac8I GCNNGC 1 cut(s) 1347
CciI TCATGA 2 cut(s) 550, 796
Cfr13I GGNCC 1 cut(s) 681
CseI GACGC 2 cut(s) 1276, 1300
CviAII CATG 5 cut(s) 356, 551, 797, 955, 1133
DdeI CTNAG 3 cut(s) 524, 1074, 1383
DpnI GATC 4 cut(s) 63, 257, 566, 1250
DpnII GATC 4 cut(s) 61, 255, 564, 1248
Eam1104I CTCTTC 2 cut(s) 321, 696
EarI CTCTTC 2 cut(s) 321, 696
Eco130I CCWWGG 2 cut(s) 514, 1218
Eco57I CTGAAG 2 cut(s) 131, 578
Eco88I CYCGRG 1 cut(s) 1172
EcoT14I CCWWGG 2 cut(s) 514, 1218
ErhI CCWWGG 2 cut(s) 514, 1218
Esp3I CGTCTC 4 cut(s) 46, 307, 1259, 1283
FaeI CATG 5 cut(s) 359, 554, 800, 958, 1136
FalI AAGNNNNNCTT 4 cut(s) 885, 917, 1202, 1234
FaqI GGGAC 1 cut(s) 311
FatI CATG 5 cut(s) 355, 550, 796, 954, 1132
FauI CCCGC 1 cut(s) 760
FbaI TGATCA 1 cut(s) 255
Fnu4HI GCNGC 6 cut(s) 460, 610, 684, 752, 1104, 1295
FokI GGATG 4 cut(s) 383, 606, 831, 1201
Fsp4HI GCNGC 6 cut(s) 460, 610, 684, 752, 1104, 1295
FspBI CTAG 7 cut(s) 23, 201, 212, 465, 515, 755, 1346
GluI GCNGC 6 cut(s) 460, 610, 684, 752, 1104, 1295
HaeIII GGCC 3 cut(s) 575, 683, 1116
HapII CCGG 1 cut(s) 194
HgaI GACGC 2 cut(s) 1276, 1300
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 5 cut(s) 359, 554, 800, 958, 1136
HpaII CCGG 1 cut(s) 194
HphI GGTGA 5 cut(s) 119, 518, 758, 1078, 1315
Hpy166II GTNNAC 1 cut(s) 1204
Hpy188I TCNGA 9 cut(s) 66, 187, 496, 631, 700, 790, 1007, 1317, 1330
Hpy8I GTNNAC 1 cut(s) 1204
HpyAV CCTTC 5 cut(s) 52, 78, 310, 438, 1120
HpyCH4III ACNGT 2 cut(s) 398, 450
HpyCH4IV ACGT 2 cut(s) 300, 933
HpyCH4V TGCA 3 cut(s) 906, 1103, 1285
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 680, 1291
HpyF3I CTNAG 3 cut(s) 524, 1074, 1383
HpySE526I ACGT 2 cut(s) 300, 933
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 5 cut(s) 359, 554, 800, 958, 1136
Ksp22I TGATCA 1 cut(s) 255
Kzo9I GATC 4 cut(s) 61, 255, 564, 1248
LmnI GCTCC 2 cut(s) 518, 1129
Lsp1109I GCAGC 5 cut(s) 471, 621, 763, 1115, 1306
LweI GCATC 2 cut(s) 133, 1279
MaeI CTAG 7 cut(s) 23, 201, 212, 465, 515, 755, 1346
MaeII ACGT 2 cut(s) 300, 933
MaeIII GTNAC 4 cut(s) 125, 347, 444, 1066
MalI GATC 4 cut(s) 63, 257, 566, 1250
MboI GATC 4 cut(s) 61, 255, 564, 1248
MboII GAAGA 6 cut(s) 338, 571, 611, 713, 823, 872
MfeI CAATTG 1 cut(s) 907
MflI RGATCY 1 cut(s) 61
MhlI GDGCHC 3 cut(s) 94, 523, 870
MluCI AATT 6 cut(s) 17, 218, 308, 625, 907, 1310
MlyI GAGTC 4 cut(s) 14, 368, 689, 1164
MmeI TCCRAC 1 cut(s) 366
MroXI GAANNNNTTC 2 cut(s) 342, 1085
MseI TTAA 6 cut(s) 117, 924, 1032, 1179, 1194, 1238
MspA1I CMGCKG 2 cut(s) 109, 686
MspI CCGG 1 cut(s) 194
MspR9I CCNGG 1 cut(s) 194
MunI CAATTG 1 cut(s) 907
Mva1269I GAATGC 1 cut(s) 241
MwoI GCNNNNNNNGC 3 cut(s) 152, 680, 1291
NciI CCSGG 1 cut(s) 194
NdeII GATC 4 cut(s) 61, 255, 564, 1248
NheI GCTAGC 1 cut(s) 1345
NlaIII CATG 5 cut(s) 359, 554, 800, 958, 1136
NlaIV GGNNCC 1 cut(s) 1088
NmuCI GTSAC 3 cut(s) 125, 444, 1066
NspV TTCGAA 1 cut(s) 431
PaeR7I CTCGAG 1 cut(s) 1172
PagI TCATGA 2 cut(s) 550, 796
PaqCI CACCTGC 1 cut(s) 1407
PctI GAATGC 1 cut(s) 241
PdmI GAANNNNTTC 2 cut(s) 342, 1085
PfeI GAWTC 7 cut(s) 172, 405, 416, 602, 833, 959, 1306
PfoI TCCNGGA 1 cut(s) 192
PkrI GCNGC 6 cut(s) 461, 611, 685, 753, 1105, 1296
PleI GAGTC 4 cut(s) 13, 367, 689, 1164
PpsI GAGTC 4 cut(s) 13, 367, 689, 1164
PshBI ATTAAT 1 cut(s) 1194
PspN4I GGNNCC 1 cut(s) 1088
PspPI GGNCC 1 cut(s) 681
PsuI RGATCY 1 cut(s) 61
PvuII CAGCTG 1 cut(s) 109
SaqAI TTAA 6 cut(s) 117, 924, 1032, 1179, 1194, 1238
SatI GCNGC 6 cut(s) 460, 610, 684, 752, 1104, 1295
Sau3AI GATC 4 cut(s) 61, 255, 564, 1248
Sau96I GGNCC 1 cut(s) 681
SchI GAGTC 4 cut(s) 14, 368, 689, 1164
ScrFI CCNGG 1 cut(s) 194
SduI GDGCHC 3 cut(s) 94, 523, 870
SfaNI GCATC 2 cut(s) 133, 1279
Sfr274I CTCGAG 1 cut(s) 1172
SfuI TTCGAA 1 cut(s) 431
SlaI CTCGAG 1 cut(s) 1172
SmlI CTYRAG 3 cut(s) 822, 1160, 1172
SmoI CTYRAG 3 cut(s) 822, 1160, 1172
SpeI ACTAGT 1 cut(s) 211
Sse9I AATT 6 cut(s) 17, 218, 308, 625, 907, 1310
SsiI CCGC 4 cut(s) 684, 767, 1186, 1349
SspMI CTAG 7 cut(s) 23, 201, 212, 465, 515, 755, 1346
StyD4I CCNGG 1 cut(s) 192
StyI CCWWGG 2 cut(s) 514, 1218
TaaI ACNGT 2 cut(s) 398, 450
TaiI ACGT 2 cut(s) 303, 936
TaqI TCGA 6 cut(s) 224, 431, 653, 915, 1173, 1251
TasI AATT 6 cut(s) 17, 218, 308, 625, 907, 1310
TauI GCSGC 1 cut(s) 686
TfiI GAWTC 7 cut(s) 172, 405, 416, 602, 833, 959, 1306
Tru1I TTAA 6 cut(s) 117, 924, 1032, 1179, 1194, 1238
Tru9I TTAA 6 cut(s) 117, 924, 1032, 1179, 1194, 1238
TscAI CASTG 2 cut(s) 453, 528
TseFI GTSAC 3 cut(s) 125, 444, 1066
TseI GCWGC 5 cut(s) 459, 609, 751, 1103, 1294
Tsp45I GTSAC 3 cut(s) 125, 444, 1066
TspDTI ATGAA 8 cut(s) 113, 148, 567, 656, 785, 813, 971, 1094
TspGWI ACGGA 1 cut(s) 70
TspRI CASTG 2 cut(s) 453, 528
VspI ATTAAT 1 cut(s) 1194
XapI RAATTY 2 cut(s) 17, 218
XbaI TCTAGA 1 cut(s) 464
XhoI CTCGAG 1 cut(s) 1172
XmaJI CCTAGG 1 cut(s) 514
XmnI GAANNNNTTC 2 cut(s) 342, 1085
XspI CTAG 7 cut(s) 23, 201, 212, 465, 515, 755, 1346
ZraI GACGTC 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.