Rw5G046150

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004521
Physical Location & Seq
Reverse (-)
80230218 .. 80231651
1434 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G046150.1

Sequence Viewer

Length: 1434 bp
ATGGAGTCCAATCATAAATTTCTAGCCAAAGAAATATATCTTTGTAAAAAGAGACGGAAGGATCTGACAAAGGCTTTAGAGCGAAGGTGTGCCCTTGCGAAACAACAGCTGAAGTTTAATCGCTGTCACCAAGAAGTAGCCGAGGCCATAAAGGCGTTCATCGAAGAACAGAATCCCTCTCCCTCTGAAACCCCGGAAGCTAGAGGCGAACTAGTTGAATCTTCGATTATGGAGAATGCTGATGAGGGTGGTTTGATCACAAGGAAACAAGAGAATAATGATGTGCCAGAAAGGAGGGACGTCTCGCAATTCTTGAAGGCATTGGAAGAGGTCGAAAAACAATTTTGTAAGTCCCATGAGTCTGGTAAGGATGTCTCAAAGAAGTTGGAAACAGACAGTATTGGAATCAAATTAGCTGCATCATCACTACCTTCGAAACTGGTCAGGTCACTGTCAAGAAGCAGCTCTAGAGTTGGTTGTTCAACCTTTTATGGTTCTGAAATAATGGGTGAAACCCTAGGAGCACTGAGGCGTTGGGAGGAGAAACTCTATCATGAACTGAAGACGATCAAAAAGGCCGAACCCAAGAGGCAGAAACCCGAAGATTCCAGCAGCGAAAACCATCCTAATTCAGAAACCGAGATGAAAATAATGTCGAATGTGAAATGTAAAGTTGCCAAAAGGGCTGCTGATAATGACTCCGAAGAGATTACAAGATTGAGAAAGGAATGTTTGCTTCCCAAACTTGGTGAGCAGCTAGATGGGTTGGCTGGAAACTGGAAAGCAATGTCAGAGTTTCATGAAAGCCAAAAGAAGATTATGTCTCAAGTAAGAGAATCTCCCAACCATCCATTACTCTGCGAAGATAAGCACCAACAAGCTACCAAAAAGCTCAAAGCACGGCTTTGCAATTGGCTCGACTGCTTTAAGTCCTACGTTTCTCTACAAAAGAAATACACGGAATCTCTTTGTTGGTGGCTCTTTCATAACGACAAACCGATAATACTCGGAGACAATGATGGTGATTGGTTGATTAACCTGAAAAATCTGAAAAGTAATACAAAGGAGGTGACTACTGAGATGAAGAGGTTCCAGAAAGATGTTGCAGCCCTATTAGGCCAACAAGGAAAGGAGCATGACCAAAAGAGGGAGATCAAGCGACTTGAGAGCCGACTCGAGATTAAGAAGCTGATGATTAATCCTGCGAACGAGAAGCAAACCTTGGAGGAGATTGAGAAGTTAAAGAAGAGGATCGAGAGAACTAAAGAGACGCATCAAAGGAAGTTGCAGGAGACGCAGCAAATGGTGAATCAATTTCAGACAGGGTTCGCTGATGTTTTCGAAAAGCTAGCGGATTTTTCAAGAAAAGCCAACGAAATCTATACTGAGCTTATCAAACACCTGCCAAAAACCCATCTGGATACGCAAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

477

Amino Acids

55.28

Weight (kDa)

9.02

Isoelectric Point (pI)

57.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 105 - 329 1.6e-30 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1410
AatII GACGTC 1 cut(s) 303
Acc36I ACCTGC 1 cut(s) 1410
AciI CCGC 1 cut(s) 1352
AclWI GGATC 2 cut(s) 69, 1259
AcsI RAATTY 1 cut(s) 17
AcuI CTGAAG 2 cut(s) 131, 581
AcyI GRCGYC 1 cut(s) 300
AfiI CCNNNNNNNGG 2 cut(s) 746, 1147
AgsI TTSAA 4 cut(s) 218, 316, 483, 1362
AhlI ACTAGT 1 cut(s) 211
AjuI GAANNNNNNNTTGG 2 cut(s) 1205, 1237
Alw21I GWGCWC 1 cut(s) 526
Alw26I GTCTC 7 cut(s) 46, 307, 379, 828, 1005, 1262, 1286
AlwI GGATC 2 cut(s) 69, 1259
Ama87I CYCGRG 1 cut(s) 1175
AoxI GGCC 3 cut(s) 144, 576, 1117
ApeKI GCWGC 7 cut(s) 416, 462, 612, 686, 754, 1106, 1297
ApoI RAATTY 1 cut(s) 17
AseI ATTAAT 1 cut(s) 1197
Asp700I GAANNNNTTC 1 cut(s) 1088
AspA2I CCTAGG 1 cut(s) 517
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 6 cut(s) 119, 521, 761, 1034, 1081, 1318
AsuII TTCGAA 2 cut(s) 434, 1341
AsuNHI GCTAGC 1 cut(s) 1348
AvaI CYCGRG 1 cut(s) 1175
AvrII CCTAGG 1 cut(s) 517
BaeGI GKGCMC 1 cut(s) 94
BbsI GAAGAC 1 cut(s) 569
Bbv12I GWGCWC 1 cut(s) 526
BbvI GCAGC 7 cut(s) 403, 474, 624, 673, 766, 1118, 1309
BccI CCATC 5 cut(s) 630, 755, 855, 1013, 1422
BceAI ACGGC 1 cut(s) 917
BcgI CGANNNNNNTGC 2 cut(s) 898, 932
BciVI GTATCC 1 cut(s) 1414
BclI TGATCA 1 cut(s) 255
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 7 cut(s) 46, 307, 379, 828, 1005, 1262, 1286
BcuI ACTAGT 1 cut(s) 211
BfaI CTAG 7 cut(s) 23, 201, 212, 468, 518, 758, 1349
BfuAI ACCTGC 1 cut(s) 1410
BfuI GTATCC 1 cut(s) 1414
BglI GCCNNNNNGGC 2 cut(s) 152, 683
BisI GCNGC 7 cut(s) 417, 463, 613, 687, 755, 1107, 1298
BlnI CCTAGG 1 cut(s) 517
BlsI GCNGC 7 cut(s) 418, 464, 614, 688, 756, 1108, 1299
Bme1390I CCNGG 1 cut(s) 194
BmeT110I CYCGRG 1 cut(s) 1175
BmiI GGNNCC 1 cut(s) 1091
BmrFI CCNGG 1 cut(s) 194
BmsI GCATC 2 cut(s) 428, 1282
BmtI GCTAGC 1 cut(s) 1352
BpiI GAAGAC 1 cut(s) 569
Bpu14I TTCGAA 2 cut(s) 434, 1341
BpuEI CTTGAG 2 cut(s) 810, 1184
BpuMI CCSGG 1 cut(s) 194
BsaHI GRCGYC 1 cut(s) 300
BsaJI CCNNGG 4 cut(s) 141, 192, 517, 1221
Bsc4I CCNNNNNNNGG 2 cut(s) 746, 1147
Bse1I ACTGG 2 cut(s) 444, 782
Bse3DI GCAATG 1 cut(s) 792
BseDI CCNNGG 4 cut(s) 141, 192, 517, 1221
BseGI GGATG 3 cut(s) 376, 622, 847
BseLI CCNNNNNNNGG 2 cut(s) 746, 1147
BseMI GCAATG 1 cut(s) 792
BseMII CTCAG 3 cut(s) 518, 1068, 1377
BseNI ACTGG 2 cut(s) 444, 782
BseRI GAGGAG 2 cut(s) 554, 1241
BseSI GKGCMC 1 cut(s) 94
BseXI GCAGC 7 cut(s) 403, 474, 624, 673, 766, 1118, 1309
BshFI GGCC 3 cut(s) 146, 578, 1119
BsiHKAI GWGCWC 1 cut(s) 526
BsiHKCI CYCGRG 1 cut(s) 1175
BsiSI CCGG 1 cut(s) 194
BslFI GGGAC 2 cut(s) 311, 337
BslI CCNNNNNNNGG 2 cut(s) 746, 1147
BsmAI GTCTC 7 cut(s) 46, 307, 379, 828, 1005, 1262, 1286
BsmBI CGTCTC 4 cut(s) 46, 307, 1262, 1286
BsmFI GGGAC 2 cut(s) 311, 337
BsmI GAATGC 1 cut(s) 241
BsnI GGCC 3 cut(s) 146, 578, 1119
BsoBI CYCGRG 1 cut(s) 1175
Bsp119I TTCGAA 2 cut(s) 434, 1341
Bsp1286I GDGCHC 2 cut(s) 94, 526
Bsp143I GATC 5 cut(s) 61, 255, 567, 1152, 1251
BspACI CCGC 1 cut(s) 1352
BspANI GGCC 3 cut(s) 146, 578, 1119
BspCNI CTCAG 3 cut(s) 519, 1069, 1378
BspHI TCATGA 2 cut(s) 553, 799
BspLI GGNNCC 1 cut(s) 1091
BspMI ACCTGC 1 cut(s) 1410
BspOI GCTAGC 1 cut(s) 1352
BspPI GGATC 2 cut(s) 69, 1259
BspT104I TTCGAA 2 cut(s) 434, 1341
BsrDI GCAATG 1 cut(s) 792
BsrI ACTGG 2 cut(s) 444, 782
BssECI CCNNGG 4 cut(s) 141, 192, 517, 1221
BssMI GATC 5 cut(s) 61, 255, 567, 1152, 1251
BssNI GRCGYC 1 cut(s) 300
BssT1I CCWWGG 2 cut(s) 517, 1221
Bst4CI ACNGT 2 cut(s) 398, 453
Bst6I CTCTTC 4 cut(s) 321, 699, 1079, 1241
BstACI GRCGYC 1 cut(s) 300
BstBI TTCGAA 2 cut(s) 434, 1341
BstC8I GCNNGC 1 cut(s) 1350
BstDEI CTNAG 3 cut(s) 527, 1077, 1386
BstF5I GGATG 3 cut(s) 376, 622, 847
BstKTI GATC 5 cut(s) 64, 258, 570, 1155, 1254
BstMAI GTCTC 7 cut(s) 46, 307, 379, 828, 1005, 1262, 1286
BstMBI GATC 5 cut(s) 61, 255, 567, 1152, 1251
BstMWI GCNNNNNNNGC 3 cut(s) 152, 683, 1294
BstSCI CCNGG 1 cut(s) 192
BstSLI GKGCMC 1 cut(s) 94
BstV1I GCAGC 7 cut(s) 403, 474, 624, 673, 766, 1118, 1309
BstV2I GAAGAC 1 cut(s) 569
BstX2I RGATCY 1 cut(s) 61
BstXI CCANNNNNNTGG 1 cut(s) 362
BstYI RGATCY 1 cut(s) 61
BsuI GTATCC 1 cut(s) 1414
BsuRI GGCC 3 cut(s) 146, 578, 1119
BtsCI GGATG 3 cut(s) 376, 622, 847
BtsIMutI CAGTG 2 cut(s) 449, 524
BveI ACCTGC 1 cut(s) 1410
Cac8I GCNNGC 1 cut(s) 1350
CciI TCATGA 2 cut(s) 553, 799
CseI GACGC 2 cut(s) 1279, 1303
CviAII CATG 4 cut(s) 356, 554, 800, 1136
DdeI CTNAG 3 cut(s) 527, 1077, 1386
DpnI GATC 5 cut(s) 63, 257, 569, 1154, 1253
DpnII GATC 5 cut(s) 61, 255, 567, 1152, 1251
Eam1104I CTCTTC 4 cut(s) 321, 699, 1079, 1241
EarI CTCTTC 4 cut(s) 321, 699, 1079, 1241
Eco130I CCWWGG 2 cut(s) 517, 1221
Eco57I CTGAAG 2 cut(s) 131, 581
Eco88I CYCGRG 1 cut(s) 1175
EcoT14I CCWWGG 2 cut(s) 517, 1221
ErhI CCWWGG 2 cut(s) 517, 1221
Esp3I CGTCTC 4 cut(s) 46, 307, 1262, 1286
FaeI CATG 4 cut(s) 359, 557, 803, 1139
FalI AAGNNNNNCTT 4 cut(s) 888, 920, 1205, 1237
FaqI GGGAC 2 cut(s) 311, 337
FatI CATG 4 cut(s) 355, 553, 799, 1135
FbaI TGATCA 1 cut(s) 255
Fnu4HI GCNGC 7 cut(s) 417, 463, 613, 687, 755, 1107, 1298
FokI GGATG 3 cut(s) 383, 609, 834
Fsp4HI GCNGC 7 cut(s) 417, 463, 613, 687, 755, 1107, 1298
FspBI CTAG 7 cut(s) 23, 201, 212, 468, 518, 758, 1349
GluI GCNGC 7 cut(s) 417, 463, 613, 687, 755, 1107, 1298
HaeIII GGCC 3 cut(s) 146, 578, 1119
HapII CCGG 1 cut(s) 194
HgaI GACGC 2 cut(s) 1279, 1303
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 4 cut(s) 359, 557, 803, 1139
HpaII CCGG 1 cut(s) 194
HphI GGTGA 6 cut(s) 119, 521, 761, 1034, 1081, 1318
Hpy188I TCNGA 9 cut(s) 66, 187, 499, 634, 703, 793, 1010, 1050, 1320
HpyAV CCTTC 4 cut(s) 52, 78, 310, 441
HpyCH4III ACNGT 2 cut(s) 398, 453
HpyCH4IV ACGT 2 cut(s) 300, 936
HpyCH4V TGCA 4 cut(s) 419, 909, 1106, 1288
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 683, 1294
HpyF3I CTNAG 3 cut(s) 527, 1077, 1386
HpySE526I ACGT 2 cut(s) 300, 936
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 4 cut(s) 359, 557, 803, 1139
Ksp22I TGATCA 1 cut(s) 255
Kzo9I GATC 5 cut(s) 61, 255, 567, 1152, 1251
LmnI GCTCC 2 cut(s) 521, 1132
Lsp1109I GCAGC 7 cut(s) 403, 474, 624, 673, 766, 1118, 1309
LweI GCATC 2 cut(s) 428, 1282
MaeI CTAG 7 cut(s) 23, 201, 212, 468, 518, 758, 1349
MaeII ACGT 2 cut(s) 300, 936
MaeIII GTNAC 3 cut(s) 125, 447, 1069
MalI GATC 5 cut(s) 63, 257, 569, 1154, 1253
MboI GATC 5 cut(s) 61, 255, 567, 1152, 1251
MfeI CAATTG 1 cut(s) 910
MflI RGATCY 1 cut(s) 61
MhlI GDGCHC 2 cut(s) 94, 526
MluCI AATT 7 cut(s) 17, 308, 341, 410, 628, 910, 1313
MlyI GAGTC 4 cut(s) 14, 368, 692, 1167
MmeI TCCRAC 1 cut(s) 366
MroXI GAANNNNTTC 1 cut(s) 1088
MseI TTAA 6 cut(s) 117, 927, 1035, 1182, 1197, 1241
MspA1I CMGCKG 1 cut(s) 109
MspI CCGG 1 cut(s) 194
MspR9I CCNGG 1 cut(s) 194
MunI CAATTG 1 cut(s) 910
Mva1269I GAATGC 1 cut(s) 241
MwoI GCNNNNNNNGC 3 cut(s) 152, 683, 1294
NciI CCSGG 1 cut(s) 194
NdeII GATC 5 cut(s) 61, 255, 567, 1152, 1251
NheI GCTAGC 1 cut(s) 1348
NlaIII CATG 4 cut(s) 359, 557, 803, 1139
NlaIV GGNNCC 1 cut(s) 1091
NmeAIII GCCGAG 1 cut(s) 166
NmuCI GTSAC 3 cut(s) 125, 447, 1069
NspV TTCGAA 2 cut(s) 434, 1341
PaeR7I CTCGAG 1 cut(s) 1175
PagI TCATGA 2 cut(s) 553, 799
PaqCI CACCTGC 1 cut(s) 1410
PctI GAATGC 1 cut(s) 241
PdmI GAANNNNTTC 1 cut(s) 1088
PfeI GAWTC 7 cut(s) 172, 218, 405, 605, 836, 962, 1309
PkrI GCNGC 7 cut(s) 418, 464, 614, 688, 756, 1108, 1299
PleI GAGTC 4 cut(s) 13, 367, 692, 1167
PpsI GAGTC 4 cut(s) 13, 367, 692, 1167
PshBI ATTAAT 1 cut(s) 1197
PspN4I GGNNCC 1 cut(s) 1091
PsuI RGATCY 1 cut(s) 61
PvuII CAGCTG 1 cut(s) 109
SaqAI TTAA 6 cut(s) 117, 927, 1035, 1182, 1197, 1241
SatI GCNGC 7 cut(s) 417, 463, 613, 687, 755, 1107, 1298
Sau3AI GATC 5 cut(s) 61, 255, 567, 1152, 1251
SchI GAGTC 4 cut(s) 14, 368, 692, 1167
ScrFI CCNGG 1 cut(s) 194
SduI GDGCHC 2 cut(s) 94, 526
SfaNI GCATC 2 cut(s) 428, 1282
Sfr274I CTCGAG 1 cut(s) 1175
SfuI TTCGAA 2 cut(s) 434, 1341
SlaI CTCGAG 1 cut(s) 1175
SmlI CTYRAG 3 cut(s) 825, 1163, 1175
SmoI CTYRAG 3 cut(s) 825, 1163, 1175
SpeI ACTAGT 1 cut(s) 211
Sse9I AATT 7 cut(s) 17, 308, 341, 410, 628, 910, 1313
SsiI CCGC 1 cut(s) 1352
SspMI CTAG 7 cut(s) 23, 201, 212, 468, 518, 758, 1349
StyD4I CCNGG 1 cut(s) 192
StyI CCWWGG 2 cut(s) 517, 1221
TaaI ACNGT 2 cut(s) 398, 453
TaiI ACGT 2 cut(s) 303, 939
TaqI TCGA 9 cut(s) 162, 224, 333, 434, 656, 918, 1176, 1254, 1341
TasI AATT 7 cut(s) 17, 308, 341, 410, 628, 910, 1313
TfiI GAWTC 7 cut(s) 172, 218, 405, 605, 836, 962, 1309
Tru1I TTAA 6 cut(s) 117, 927, 1035, 1182, 1197, 1241
Tru9I TTAA 6 cut(s) 117, 927, 1035, 1182, 1197, 1241
TscAI CASTG 2 cut(s) 456, 531
TseFI GTSAC 3 cut(s) 125, 447, 1069
TseI GCWGC 7 cut(s) 416, 462, 612, 686, 754, 1106, 1297
Tsp45I GTSAC 3 cut(s) 125, 447, 1069
TspDTI ATGAA 7 cut(s) 148, 570, 659, 788, 816, 974, 1097
TspGWI ACGGA 2 cut(s) 70, 974
TspRI CASTG 2 cut(s) 456, 531
VspI ATTAAT 1 cut(s) 1197
XapI RAATTY 1 cut(s) 17
XbaI TCTAGA 1 cut(s) 467
XhoI CTCGAG 1 cut(s) 1175
XmaJI CCTAGG 1 cut(s) 517
XmnI GAANNNNTTC 1 cut(s) 1088
XspI CTAG 7 cut(s) 23, 201, 212, 468, 518, 758, 1349
ZraI GACGTC 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.