MD02G1031400.v1.1

Heme oxygenase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005358
Physical Location & Seq
Forward (+)
2457972 .. 2460770
2799 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1031400.v1.1.491

Sequence Viewer

Length: 852 bp
ATGGCTTCTTTAACACCGGTTTCGCAATCCCAATTGCTTTTCAACAAAACCCAGTTCAAGTTACCACCAAATTTGAGGTCCGGTTTATTGGTGAACAAGTTCTCTGTTTCGTTCCGGAGGATGAGTTTCAGAGTGCCCACGAAGGCGGCGGCGGCGTCCGGGGTGGTTTCCGCTACCACGGCCGAGAAGGCGAAGAAGAGGTACCCGGGGGAGGCGAAGGGGTTCGTGGAGGAGATGAGGTTTGTGGCGATGAAGTTGCACACCAGGGACCAGGCGAAGGAGGGGGAGAAGGAGGTGAAGGAGCCGCAGGAGGGGCCGGTGGCCAAGTGGGAGCCAACAGTCGATGGGTATTTGAAGTTTTTGGTGGATAGCAAGTTGGTTTATGATACCCTTGAAGAGATTGTTGAAAAAGCTCCATTTCCTTCTTACAACAAGTTCAGAAATACTGGATTGGAAAGGTCCGAAAAGTTGGCAAAAGATTTGGAGTGGTTTAAGGAGCAAGGTTATGCCATACCAGAACCTTCTACTCCTGGTGTTACGTACGCGGAGTATCTTAAGGAACTGTCGGAGAAGGATCCTCAAGCATTTATTTGCCACTTCTACAATATATACTTCGCTCATTCAGCTGGCGGGCGAATGATTGGGAAAAAGGTGGCGGAAATGATACTAGACAAAAAGGACTTGGAATTCTACAAATGGGACGGCGAGCTTCCCCAACTGCTGCAGAATGTGAGGGAGAAGTTGAATAAAGTTGCAGAGAGCTGGACGAGGGAGGAGAAGAACCACTGCTTGGAAGAAACCGAGAAATCGTTCAAACATTCGGGAGAGATTCTGCGTCTCATATTATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004392 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006629 GO:0006720 GO:0006721 GO:0006725 GO:0006778 GO:0006787 GO:0006788 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009058 GO:0009314 GO:0009411 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009648 GO:0009791 GO:0009812 GO:0009813 GO:0009888 GO:0009987 GO:0010019 GO:0010024 GO:0010073 GO:0010075 GO:0010119 GO:0010225 GO:0010228 GO:0010229 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0016491 GO:0016705 GO:0018130 GO:0019438 GO:0019439 GO:0020037 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0033013 GO:0033014 GO:0033015 GO:0034641 GO:0034644 GO:0040008 GO:0042168 GO:0042440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044270 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046148 GO:0046483 GO:0046700 GO:0046906 GO:0048037 GO:0048367 GO:0048507 GO:0048509 GO:0048573 GO:0048608 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051186 GO:0051187 GO:0051188 GO:0051202 GO:0051716 GO:0055114 GO:0061458 GO:0065007 GO:0071214 GO:0071478 GO:0071482 GO:0071494 GO:0071704 GO:0090567 GO:0097159 GO:0104004 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00860 ko01100 ko01110 map00860 map01100 map01110
Pfam Domains
Protein Families
Heme_oxygenase

Protein Analysis

284

Amino Acids

32.43

Weight (kDa)

8.76

Isoelectric Point (pI)

48.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Heme_oxygenase PF01126 94 - 277 1e-13 Heme oxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 201
AccB1I GGYRCC 1 cut(s) 201
AccB7I CCANNNNNTGG 1 cut(s) 790
AccII CGCG 1 cut(s) 545
AccIII TCCGGA 1 cut(s) 114
AciI CCGC 8 cut(s) 146, 149, 152, 171, 305, 545, 630, 656
AclWI GGATC 2 cut(s) 569, 582
AcoI YGGCCR 2 cut(s) 180, 321
AcsI RAATTY 2 cut(s) 70, 686
AcyI GRCGYC 1 cut(s) 155
AfaI GTAC 2 cut(s) 203, 542
AfiI CCNNNNNNNGG 4 cut(s) 211, 277, 311, 790
AflII CTTAAG 1 cut(s) 554
AgeI ACCGGT 1 cut(s) 16
AgsI TTSAA 7 cut(s) 43, 58, 355, 395, 407, 745, 814
AjnI CCWGG 3 cut(s) 263, 270, 529
AjuI GAANNNNNNNTTGG 2 cut(s) 773, 805
AluBI AGCT 4 cut(s) 413, 626, 709, 762
AluI AGCT 4 cut(s) 413, 626, 709, 762
Alw26I GTCTC 1 cut(s) 842
AlwI GGATC 2 cut(s) 569, 582
Ama87I CYCGRG 1 cut(s) 205
Aor13HI TCCGGA 1 cut(s) 114
AoxI GGCC 3 cut(s) 180, 314, 321
ApeKI GCWGC 1 cut(s) 721
ApoI RAATTY 2 cut(s) 70, 686
ArsI GACNNNNNNTTYG 2 cut(s) 62, 94
AsiGI ACCGGT 1 cut(s) 16
Asp700I GAANNNNTTC 3 cut(s) 98, 221, 809
Asp718I GGTACC 1 cut(s) 201
AspS9I GGNCC 4 cut(s) 78, 268, 314, 459
AsuC2I CCSGG 3 cut(s) 160, 206, 207
AsuHPI GGTGA 2 cut(s) 103, 307
AvaI CYCGRG 1 cut(s) 205
AvaII GGWCC 3 cut(s) 78, 268, 459
BaeGI GKGCMC 1 cut(s) 138
BalI TGGCCA 1 cut(s) 323
BamHI GGATCC 1 cut(s) 574
BanI GGYRCC 1 cut(s) 201
BarI GAAGNNNNNNTAC 2 cut(s) 185, 217
BbvI GCAGC 1 cut(s) 708
BccI CCATC 1 cut(s) 338
BceAI ACGGC 2 cut(s) 195, 718
BcgI CGANNNNNNTGC 2 cut(s) 238, 272
BciT130I CCWGG 3 cut(s) 265, 272, 531
BcnI CCSGG 3 cut(s) 160, 206, 207
BcoDI GTCTC 1 cut(s) 842
BfaI CTAG 1 cut(s) 668
BfmI CTRYAG 1 cut(s) 722
BfrI CTTAAG 1 cut(s) 554
BglI GCCNNNNNGGC 1 cut(s) 188
BisI GCNGC 5 cut(s) 147, 150, 153, 305, 722
BlsI GCNGC 5 cut(s) 148, 151, 154, 306, 723
Bme1390I CCNGG 6 cut(s) 160, 206, 207, 265, 272, 531
Bme18I GGWCC 3 cut(s) 78, 268, 459
BmeT110I CYCGRG 1 cut(s) 205
BmgT120I GGNCC 4 cut(s) 78, 268, 314, 459
BmiI GGNNCC 6 cut(s) 203, 269, 303, 315, 333, 576
BmrFI CCNGG 6 cut(s) 160, 206, 207, 265, 272, 531
BmrI ACTGGG 1 cut(s) 46
BmuI ACTGGG 1 cut(s) 46
BpuEI CTTGAG 1 cut(s) 564
BpuMI CCSGG 3 cut(s) 160, 206, 207
BsaAI YACGTR 1 cut(s) 540
BsaHI GRCGYC 1 cut(s) 155
BsaJI CCNNGG 5 cut(s) 159, 177, 205, 206, 264
BsaWI WCCGGW 3 cut(s) 16, 80, 114
BsaXI ACNNNNNCTCC 4 cut(s) 109, 139, 224, 254
Bsc4I CCNNNNNNNGG 4 cut(s) 211, 277, 311, 790
Bse118I RCCGGY 2 cut(s) 16, 316
Bse1I ACTGG 2 cut(s) 52, 451
BseAI TCCGGA 1 cut(s) 114
BseBI CCWGG 3 cut(s) 265, 272, 531
BseDI CCNNGG 5 cut(s) 159, 177, 205, 206, 264
BseGI GGATG 1 cut(s) 126
BseLI CCNNNNNNNGG 4 cut(s) 211, 277, 311, 790
BseNI ACTGG 2 cut(s) 52, 451
BseRI GAGGAG 2 cut(s) 245, 788
BseSI GKGCMC 1 cut(s) 138
BseX3I CGGCCG 1 cut(s) 180
BseXI GCAGC 1 cut(s) 708
Bsh1236I CGCG 1 cut(s) 545
Bsh1285I CGRYCG 1 cut(s) 183
BshFI GGCC 3 cut(s) 182, 316, 323
BshNI GGYRCC 1 cut(s) 201
BshTI ACCGGT 1 cut(s) 16
BsiEI CGRYCG 1 cut(s) 183
BsiHKCI CYCGRG 1 cut(s) 205
BsiSI CCGG 6 cut(s) 17, 81, 115, 159, 206, 317
BsiWI CGTACG 1 cut(s) 540
BslFI GGGAC 2 cut(s) 281, 713
BslI CCNNNNNNNGG 4 cut(s) 211, 277, 311, 790
BsmAI GTCTC 1 cut(s) 842
BsmBI CGTCTC 1 cut(s) 842
BsmFI GGGAC 2 cut(s) 281, 713
BsnI GGCC 3 cut(s) 182, 316, 323
BsoBI CYCGRG 1 cut(s) 205
Bsp1286I GDGCHC 1 cut(s) 138
Bsp13I TCCGGA 1 cut(s) 114
Bsp143I GATC 1 cut(s) 574
BspACI CCGC 8 cut(s) 146, 149, 152, 171, 305, 545, 630, 656
BspANI GGCC 3 cut(s) 182, 316, 323
BspEI TCCGGA 1 cut(s) 114
BspFNI CGCG 1 cut(s) 545
BspHI TCATGA 1 cut(s) 848
BspLI GGNNCC 6 cut(s) 203, 269, 303, 315, 333, 576
BspMAI CTGCAG 1 cut(s) 726
BspPI GGATC 2 cut(s) 569, 582
BspT107I GGYRCC 1 cut(s) 201
BspTI CTTAAG 1 cut(s) 554
BsrFI RCCGGY 2 cut(s) 16, 316
BsrI ACTGG 2 cut(s) 52, 451
BssAI RCCGGY 2 cut(s) 16, 316
BssECI CCNNGG 5 cut(s) 159, 177, 205, 206, 264
BssMI GATC 1 cut(s) 574
BssNI GRCGYC 1 cut(s) 155
Bst2UI CCWGG 3 cut(s) 265, 272, 531
Bst4CI ACNGT 2 cut(s) 340, 564
Bst6I CTCTTC 2 cut(s) 191, 390
BstACI GRCGYC 1 cut(s) 155
BstAFI CTTAAG 1 cut(s) 554
BstBAI YACGTR 1 cut(s) 540
BstC8I GCNNGC 3 cut(s) 628, 632, 707
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 1 cut(s) 126
BstFNI CGCG 1 cut(s) 545
BstKTI GATC 1 cut(s) 577
BstMAI GTCTC 1 cut(s) 842
BstMBI GATC 1 cut(s) 574
BstMCI CGRYCG 1 cut(s) 183
BstMWI GCNNNNNNNGC 5 cut(s) 152, 179, 188, 313, 623
BstNI CCWGG 3 cut(s) 265, 272, 531
BstSCI CCNGG 6 cut(s) 158, 204, 205, 263, 270, 529
BstSFI CTRYAG 1 cut(s) 722
BstSLI GKGCMC 1 cut(s) 138
BstSNI TACGTA 1 cut(s) 540
BstUI CGCG 1 cut(s) 545
BstV1I GCAGC 1 cut(s) 708
BstX2I RGATCY 1 cut(s) 574
BstYI RGATCY 1 cut(s) 574
BstZI CGGCCG 1 cut(s) 180
BsuRI GGCC 3 cut(s) 182, 316, 323
BtgI CCRYGG 1 cut(s) 177
BtgZI GCGATG 1 cut(s) 263
BtsCI GGATG 1 cut(s) 126
BtsI GCAGTG 1 cut(s) 784
BtsIMutI CAGTG 1 cut(s) 784
Cac8I GCNNGC 3 cut(s) 628, 632, 707
CciI TCATGA 1 cut(s) 848
Cfr10I RCCGGY 2 cut(s) 16, 316
Cfr13I GGNCC 4 cut(s) 78, 268, 314, 459
Cfr9I CCCGGG 1 cut(s) 205
CseI GACGC 2 cut(s) 144, 824
Csp6I GTAC 2 cut(s) 202, 541
CspAI ACCGGT 1 cut(s) 16
CspCI CAANNNNNGTGG 2 cut(s) 54, 89
CviAII CATG 1 cut(s) 849
CviQI GTAC 2 cut(s) 202, 541
DpnI GATC 1 cut(s) 576
DpnII GATC 1 cut(s) 574
EaeI YGGCCR 2 cut(s) 180, 321
EagI CGGCCG 1 cut(s) 180
Eam1104I CTCTTC 2 cut(s) 191, 390
EarI CTCTTC 2 cut(s) 191, 390
EciI GGCGGA 1 cut(s) 671
EclXI CGGCCG 1 cut(s) 180
Eco105I TACGTA 1 cut(s) 540
Eco47I GGWCC 3 cut(s) 78, 268, 459
Eco52I CGGCCG 1 cut(s) 180
Eco88I CYCGRG 1 cut(s) 205
EcoRI GAATTC 1 cut(s) 686
EcoRII CCWGG 3 cut(s) 263, 270, 529
Esp3I CGTCTC 1 cut(s) 842
FaeI CATG 1 cut(s) 852
FaiI YATR 7 cut(s) 384, 507, 512, 608, 610, 842, 850
FaqI GGGAC 2 cut(s) 281, 713
FatI CATG 1 cut(s) 848
FauI CCCGC 1 cut(s) 623
Fnu4HI GCNGC 5 cut(s) 147, 150, 153, 305, 722
FokI GGATG 1 cut(s) 133
Fsp4HI GCNGC 5 cut(s) 147, 150, 153, 305, 722
FspBI CTAG 1 cut(s) 668
GluI GCNGC 5 cut(s) 147, 150, 153, 305, 722
HaeIII GGCC 3 cut(s) 182, 316, 323
HapII CCGG 6 cut(s) 17, 81, 115, 159, 206, 317
HgaI GACGC 2 cut(s) 144, 824
Hin1I GRCGYC 1 cut(s) 155
Hin1II CATG 1 cut(s) 852
HinfI GANTC 1 cut(s) 829
HpaII CCGG 6 cut(s) 17, 81, 115, 159, 206, 317
HphI GGTGA 2 cut(s) 103, 307
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 4 cut(s) 131, 440, 463, 568
Hpy188III TCNNGA 3 cut(s) 115, 822, 849
Hpy8I GTNNAC 1 cut(s) 94
HpyAV CCTTC 9 cut(s) 136, 181, 211, 271, 283, 292, 432, 531, 565
HpyCH4III ACNGT 2 cut(s) 340, 564
HpyCH4IV ACGT 1 cut(s) 539
HpyCH4V TGCA 3 cut(s) 259, 724, 755
HpyF10VI GCNNNNNNNGC 5 cut(s) 152, 179, 188, 313, 623
HpySE526I ACGT 1 cut(s) 539
Hsp92I GRCGYC 1 cut(s) 155
Hsp92II CATG 1 cut(s) 852
Kpn2I TCCGGA 1 cut(s) 114
KpnI GGTACC 1 cut(s) 205
Kzo9I GATC 1 cut(s) 574
LmnI GCTCC 4 cut(s) 301, 331, 418, 496
Lsp1109I GCAGC 1 cut(s) 708
MaeI CTAG 1 cut(s) 668
MaeII ACGT 1 cut(s) 539
MaeIII GTNAC 2 cut(s) 60, 535
MalI GATC 1 cut(s) 576
MboI GATC 1 cut(s) 574
MboII GAAGA 5 cut(s) 205, 208, 407, 790, 806
MfeI CAATTG 1 cut(s) 32
MflI RGATCY 1 cut(s) 574
MhlI GDGCHC 1 cut(s) 138
MlsI TGGCCA 1 cut(s) 323
MluCI AATT 3 cut(s) 32, 70, 686
MluNI TGGCCA 1 cut(s) 323
MmeI TCCRAC 1 cut(s) 546
Mox20I TGGCCA 1 cut(s) 323
MroI TCCGGA 1 cut(s) 114
MroXI GAANNNNTTC 3 cut(s) 98, 221, 809
MscI TGGCCA 1 cut(s) 323
MseI TTAA 3 cut(s) 11, 492, 555
Msp20I TGGCCA 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 626
MspCI CTTAAG 1 cut(s) 554
MspI CCGG 6 cut(s) 17, 81, 115, 159, 206, 317
MspR9I CCNGG 6 cut(s) 160, 206, 207, 265, 272, 531
MunI CAATTG 1 cut(s) 32
MvaI CCWGG 3 cut(s) 265, 272, 531
MvnI CGCG 1 cut(s) 545
MwoI GCNNNNNNNGC 5 cut(s) 152, 179, 188, 313, 623
NciI CCSGG 3 cut(s) 160, 206, 207
NdeII GATC 1 cut(s) 574
NlaIII CATG 1 cut(s) 852
NlaIV GGNNCC 6 cut(s) 203, 269, 303, 315, 333, 576
NmeAIII GCCGAG 1 cut(s) 208
PagI TCATGA 1 cut(s) 848
PdmI GAANNNNTTC 3 cut(s) 98, 221, 809
PfeI GAWTC 1 cut(s) 829
Pfl23II CGTACG 1 cut(s) 540
PflMI CCANNNNNTGG 1 cut(s) 790
PinAI ACCGGT 1 cut(s) 16
PkrI GCNGC 5 cut(s) 148, 151, 154, 306, 723
Ppu21I YACGTR 1 cut(s) 540
Psp6I CCWGG 3 cut(s) 263, 270, 529
PspGI CCWGG 3 cut(s) 263, 270, 529
PspLI CGTACG 1 cut(s) 540
PspN4I GGNNCC 6 cut(s) 203, 269, 303, 315, 333, 576
PspPI GGNCC 4 cut(s) 78, 268, 314, 459
PstI CTGCAG 1 cut(s) 726
PsuI RGATCY 1 cut(s) 574
PvuII CAGCTG 1 cut(s) 626
RsaI GTAC 2 cut(s) 203, 542
RsaNI GTAC 2 cut(s) 202, 541
SaqAI TTAA 3 cut(s) 11, 492, 555
SatI GCNGC 5 cut(s) 147, 150, 153, 305, 722
Sau3AI GATC 1 cut(s) 574
Sau96I GGNCC 4 cut(s) 78, 268, 314, 459
ScrFI CCNGG 6 cut(s) 160, 206, 207, 265, 272, 531
SduI GDGCHC 1 cut(s) 138
SfcI CTRYAG 1 cut(s) 722
SinI GGWCC 3 cut(s) 78, 268, 459
SmaI CCCGGG 1 cut(s) 207
SmlI CTYRAG 2 cut(s) 554, 579
SmoI CTYRAG 2 cut(s) 554, 579
SnaBI TACGTA 1 cut(s) 540
Sse9I AATT 3 cut(s) 32, 70, 686
SsiI CCGC 8 cut(s) 146, 149, 152, 171, 305, 545, 630, 656
SspMI CTAG 1 cut(s) 668
StyD4I CCNGG 6 cut(s) 158, 204, 205, 263, 270, 529
TaaI ACNGT 2 cut(s) 340, 564
TaiI ACGT 1 cut(s) 542
TaqI TCGA 1 cut(s) 342
TasI AATT 3 cut(s) 32, 70, 686
TauI GCSGC 4 cut(s) 149, 152, 155, 307
TfiI GAWTC 1 cut(s) 829
Tru1I TTAA 3 cut(s) 11, 492, 555
Tru9I TTAA 3 cut(s) 11, 492, 555
TscAI CASTG 1 cut(s) 791
TseI GCWGC 1 cut(s) 721
TspDTI ATGAA 1 cut(s) 266
TspMI CCCGGG 1 cut(s) 205
TspRI CASTG 1 cut(s) 791
Van91I CCANNNNNTGG 1 cut(s) 790
Vha464I CTTAAG 1 cut(s) 554
VpaK11BI GGWCC 3 cut(s) 78, 268, 459
XapI RAATTY 2 cut(s) 70, 686
XmaI CCCGGG 1 cut(s) 205
XmnI GAANNNNTTC 3 cut(s) 98, 221, 809
XspI CTAG 1 cut(s) 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.