Rh2DG038300

Heme oxygenase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0005358
Physical Location & Seq
Forward (+)
2657917 .. 2660749
2833 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG038300.1

Sequence Viewer

Length: 969 bp
ATGGCTTCTTTAACCCCAATTTCGCAATCCCAGTCCCTTTTTAACAAGACCCCGTTCCTCGCTAACCCCAGTTTAGGGTCTGATTTTTTGCCCAAGAAATTCTCCGTTTCGTTTCCCAAGATGACTACCAGGTTTCCCGTGAAGGCGGCTGCCGCCGGAGTTGTGTCGGCGACGACGGCGGAGATGCCGAAGAAGAGGTACCCCGGAGAGGCAAAAGGGTTTGTGGAGGAGATGAGGTTTGTGGCAATGAAGTTGCATACCAGGGACCAGGCTAAGGAGGGTGAGAAGGAGGTGAAGGAGCCTCAGGAGAGGCCGGTGGCCAAGTGGGACCCGACGCTTGATGGGTACTTGAGGTTTCTTGTGGATAGCAAGTTGGTGTATGACACCCTTGAGGGGATTGTCGACAAGGCTCCCTTCCCTTCTTATGCTGAGTTTAGAGATACTGGATTGGAAAGGTCCGAAAAGTTGGCAAAAGATTTGGAGTGGTTCAAGGAGCAAGGCCATGCCATACCAGAACCTTCTGCTCCTGGTGTAACCTATGCTGAGTATCTTAAGGAACTGTCAGAGAAGGATCCTCAAGCATTTCTCTGCCACTTCTACAATATATACTTTGCTCATTCAGCTGGTGGACGAATGATTGGGAAAAAGGTGGCTGAAATGATCCTCGACAAGAAAGATTTGGAATTCTATAAATGGGATGGTGAACTTTCCCAATTGTTGCAGAATGTGAGGGAGAAATTGAACGAAGTTGCAGAGGTAAGTTCCTTGCCTTTTGATTGCAAAACATGTCTCGTCTGCATCTCTTTGCTTGATCTGACTCTATATATTATTTATATGCATAATGCATCAGAGCTGGACGAGGGAGGAGAAAAACCATTGCTTGGAAGAAACAGAGAAATCATTCAAACATTCAGGGGAAATTCTCCGTCTAATATTGTCATGATGGTGCTGCTGCTACTGCTGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004392 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006629 GO:0006720 GO:0006721 GO:0006725 GO:0006778 GO:0006787 GO:0006788 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009058 GO:0009314 GO:0009411 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009648 GO:0009791 GO:0009812 GO:0009813 GO:0009888 GO:0009987 GO:0010019 GO:0010024 GO:0010073 GO:0010075 GO:0010119 GO:0010225 GO:0010228 GO:0010229 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0016491 GO:0016705 GO:0018130 GO:0019438 GO:0019439 GO:0020037 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0033013 GO:0033014 GO:0033015 GO:0034641 GO:0034644 GO:0040008 GO:0042168 GO:0042440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044270 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046148 GO:0046483 GO:0046700 GO:0046906 GO:0048037 GO:0048367 GO:0048507 GO:0048509 GO:0048573 GO:0048608 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051186 GO:0051187 GO:0051188 GO:0051202 GO:0051716 GO:0055114 GO:0061458 GO:0065007 GO:0071214 GO:0071478 GO:0071482 GO:0071494 GO:0071704 GO:0090567 GO:0097159 GO:0104004 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00860 ko01100 ko01110 map00860 map01100 map01110
Pfam Domains
Protein Families
Heme_oxygenase

Protein Analysis

322

Amino Acids

36.36

Weight (kDa)

5.88

Isoelectric Point (pI)

46.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Heme_oxygenase PF01126 113 - 250 7.4e-10 Heme oxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 198
AccB1I GGYRCC 1 cut(s) 198
AccB7I CCANNNNNTGG 1 cut(s) 881
AccI GTMKAC 1 cut(s) 402
AciI CCGC 3 cut(s) 146, 153, 179
AclWI GGATC 3 cut(s) 566, 579, 655
AcoI YGGCCR 1 cut(s) 318
AcsI RAATTY 3 cut(s) 98, 683, 919
AfaI GTAC 2 cut(s) 200, 347
AfiI CCNNNNNNNGG 6 cut(s) 74, 75, 208, 274, 393, 881
AflII CTTAAG 1 cut(s) 551
AflIII ACRYGT 1 cut(s) 785
AgsI TTSAA 3 cut(s) 490, 742, 905
AjnI CCWGG 4 cut(s) 128, 260, 267, 526
AluBI AGCT 2 cut(s) 623, 853
AluI AGCT 2 cut(s) 623, 853
Alw26I GTCTC 1 cut(s) 794
AlwI GGATC 3 cut(s) 566, 579, 655
AoxI GGCC 3 cut(s) 311, 318, 499
ApeKI GCWGC 3 cut(s) 149, 949, 952
ApoI RAATTY 3 cut(s) 98, 683, 919
Asp700I GAANNNNTTC 1 cut(s) 900
Asp718I GGTACC 1 cut(s) 198
AspS9I GGNCC 3 cut(s) 265, 328, 456
AsuC2I CCSGG 1 cut(s) 204
AsuHPI GGTGA 3 cut(s) 293, 304, 713
AvaII GGWCC 3 cut(s) 265, 328, 456
AxyI CCTNAGG 1 cut(s) 303
BalI TGGCCA 1 cut(s) 320
BamHI GGATCC 1 cut(s) 571
BanI GGYRCC 1 cut(s) 198
BarI GAAGNNNNNNTAC 2 cut(s) 182, 214
BbvI GCAGC 3 cut(s) 136, 936, 939
BccI CCATC 3 cut(s) 335, 692, 937
BceAI ACGGC 1 cut(s) 192
BciT130I CCWGG 4 cut(s) 130, 262, 269, 528
BcnI CCSGG 1 cut(s) 204
BcoDI GTCTC 1 cut(s) 794
BfrI CTTAAG 1 cut(s) 551
BisI GCNGC 5 cut(s) 147, 150, 153, 950, 953
BlsI GCNGC 5 cut(s) 148, 151, 154, 951, 954
Bme1390I CCNGG 5 cut(s) 130, 204, 262, 269, 528
Bme18I GGWCC 3 cut(s) 265, 328, 456
BmgT120I GGNCC 3 cut(s) 265, 328, 456
BmiI GGNNCC 7 cut(s) 200, 266, 300, 329, 330, 411, 573
BmrFI CCNGG 5 cut(s) 130, 204, 262, 269, 528
BmrI ACTGGG 2 cut(s) 25, 63
BmsI GCATC 3 cut(s) 174, 807, 854
BmuI ACTGGG 2 cut(s) 25, 63
Bpu10I CCTNAGC 1 cut(s) 273
BpuEI CTTGAG 3 cut(s) 370, 410, 561
BpuMI CCSGG 1 cut(s) 204
BsaJI CCNNGG 2 cut(s) 202, 261
BsaXI ACNNNNNCTCC 2 cut(s) 221, 251
Bsc4I CCNNNNNNNGG 6 cut(s) 74, 75, 208, 274, 393, 881
Bse118I RCCGGY 1 cut(s) 313
Bse1I ACTGG 3 cut(s) 31, 69, 448
Bse21I CCTNAGG 1 cut(s) 303
Bse3DI GCAATG 2 cut(s) 252, 875
BseBI CCWGG 4 cut(s) 130, 262, 269, 528
BseDI CCNNGG 2 cut(s) 202, 261
BseGI GGATG 1 cut(s) 703
BseLI CCNNNNNNNGG 6 cut(s) 74, 75, 208, 274, 393, 881
BseMI GCAATG 2 cut(s) 252, 875
BseMII CTCAG 3 cut(s) 317, 420, 534
BseNI ACTGG 3 cut(s) 31, 69, 448
BseRI GAGGAG 2 cut(s) 242, 879
BseXI GCAGC 3 cut(s) 136, 936, 939
BshFI GGCC 3 cut(s) 313, 320, 501
BshNI GGYRCC 1 cut(s) 198
BsiSI CCGG 3 cut(s) 156, 204, 314
BslFI GGGAC 3 cut(s) 19, 278, 341
BslI CCNNNNNNNGG 6 cut(s) 74, 75, 208, 274, 393, 881
BsmAI GTCTC 1 cut(s) 794
BsmFI GGGAC 3 cut(s) 19, 278, 341
BsnI GGCC 3 cut(s) 313, 320, 501
Bsp143I GATC 3 cut(s) 571, 660, 811
BspACI CCGC 3 cut(s) 146, 153, 179
BspANI GGCC 3 cut(s) 313, 320, 501
BspCNI CTCAG 3 cut(s) 316, 421, 535
BspHI TCATGA 1 cut(s) 939
BspLI GGNNCC 7 cut(s) 200, 266, 300, 329, 330, 411, 573
BspPI GGATC 3 cut(s) 566, 579, 655
BspT107I GGYRCC 1 cut(s) 198
BspTI CTTAAG 1 cut(s) 551
BsrDI GCAATG 2 cut(s) 252, 875
BsrFI RCCGGY 1 cut(s) 313
BsrI ACTGG 3 cut(s) 31, 69, 448
BssAI RCCGGY 1 cut(s) 313
BssECI CCNNGG 2 cut(s) 202, 261
BssMI GATC 3 cut(s) 571, 660, 811
Bst2UI CCWGG 4 cut(s) 130, 262, 269, 528
Bst4CI ACNGT 1 cut(s) 561
Bst6I CTCTTC 1 cut(s) 188
BstAFI CTTAAG 1 cut(s) 551
BstDEI CTNAG 4 cut(s) 273, 303, 429, 543
BstF5I GGATG 1 cut(s) 703
BstKTI GATC 3 cut(s) 574, 663, 814
BstMAI GTCTC 1 cut(s) 794
BstMBI GATC 3 cut(s) 571, 660, 811
BstMWI GCNNNNNNNGC 4 cut(s) 152, 176, 620, 958
BstNI CCWGG 4 cut(s) 130, 262, 269, 528
BstNSI RCATGY 1 cut(s) 789
BstSCI CCNGG 5 cut(s) 128, 202, 260, 267, 526
BstV1I GCAGC 3 cut(s) 136, 936, 939
BstX2I RGATCY 1 cut(s) 571
BstYI RGATCY 1 cut(s) 571
Bsu36I CCTNAGG 1 cut(s) 303
BsuRI GGCC 3 cut(s) 313, 320, 501
BtsCI GGATG 1 cut(s) 703
CciI TCATGA 1 cut(s) 939
Cfr10I RCCGGY 1 cut(s) 313
Cfr13I GGNCC 3 cut(s) 265, 328, 456
CseI GACGC 1 cut(s) 343
CsiI ACCWGGT 1 cut(s) 128
Csp6I GTAC 2 cut(s) 199, 346
CviAII CATG 3 cut(s) 503, 786, 940
CviQI GTAC 2 cut(s) 199, 346
DdeI CTNAG 4 cut(s) 273, 303, 429, 543
DpnI GATC 3 cut(s) 573, 662, 813
DpnII GATC 3 cut(s) 571, 660, 811
EaeI YGGCCR 1 cut(s) 318
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
EciI GGCGGA 1 cut(s) 194
Eco47I GGWCC 3 cut(s) 265, 328, 456
Eco81I CCTNAGG 1 cut(s) 303
EcoO109I RGGNCCY 1 cut(s) 328
EcoRI GAATTC 1 cut(s) 683
EcoRII CCWGG 4 cut(s) 128, 260, 267, 526
EcoT22I ATGCAT 2 cut(s) 840, 847
FaeI CATG 3 cut(s) 506, 789, 943
FalI AAGNNNNNCTT 2 cut(s) 398, 430
FaqI GGGAC 3 cut(s) 19, 278, 341
FatI CATG 3 cut(s) 502, 785, 939
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 5 cut(s) 147, 150, 153, 950, 953
FokI GGATG 1 cut(s) 710
Fsp4HI GCNGC 5 cut(s) 147, 150, 153, 950, 953
GluI GCNGC 5 cut(s) 147, 150, 153, 950, 953
HaeIII GGCC 3 cut(s) 313, 320, 501
HapII CCGG 3 cut(s) 156, 204, 314
HgaI GACGC 1 cut(s) 343
Hin1II CATG 3 cut(s) 506, 789, 943
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HinfI GANTC 1 cut(s) 817
HpaII CCGG 3 cut(s) 156, 204, 314
HphI GGTGA 3 cut(s) 293, 304, 713
Hpy166II GTNNAC 3 cut(s) 403, 629, 704
Hpy188I TCNGA 5 cut(s) 82, 460, 565, 816, 850
Hpy188III TCNNGA 2 cut(s) 305, 940
Hpy8I GTNNAC 3 cut(s) 403, 629, 704
Hpy99I CGWCG 3 cut(s) 175, 178, 337
HpyAV CCTTC 7 cut(s) 136, 280, 289, 424, 429, 528, 562
HpyCH4III ACNGT 1 cut(s) 561
HpyCH4V TGCA 7 cut(s) 256, 721, 752, 780, 798, 838, 845
HpyF10VI GCNNNNNNNGC 4 cut(s) 152, 176, 620, 958
HpyF3I CTNAG 4 cut(s) 273, 303, 429, 543
Hsp92II CATG 3 cut(s) 506, 789, 943
KflI GGGWCCC 1 cut(s) 328
KpnI GGTACC 1 cut(s) 202
Kzo9I GATC 3 cut(s) 571, 660, 811
LmnI GCTCC 4 cut(s) 298, 415, 493, 529
Lsp1109I GCAGC 3 cut(s) 136, 936, 939
LweI GCATC 3 cut(s) 174, 807, 854
MabI ACCWGGT 1 cut(s) 128
MaeIII GTNAC 1 cut(s) 532
MalI GATC 3 cut(s) 573, 662, 813
MboI GATC 3 cut(s) 571, 660, 811
MboII GAAGA 3 cut(s) 202, 205, 897
MfeI CAATTG 1 cut(s) 713
MflI RGATCY 1 cut(s) 571
MlsI TGGCCA 1 cut(s) 320
MluCI AATT 6 cut(s) 18, 98, 683, 713, 737, 919
MluNI TGGCCA 1 cut(s) 320
MlyI GAGTC 1 cut(s) 811
Mox20I TGGCCA 1 cut(s) 320
Mph1103I ATGCAT 2 cut(s) 840, 847
MroXI GAANNNNTTC 1 cut(s) 900
MscI TGGCCA 1 cut(s) 320
MseI TTAA 3 cut(s) 11, 42, 552
MslI CAYNNNNRTG 1 cut(s) 944
Msp20I TGGCCA 1 cut(s) 320
MspA1I CMGCKG 1 cut(s) 623
MspCI CTTAAG 1 cut(s) 551
MspI CCGG 3 cut(s) 156, 204, 314
MspR9I CCNGG 5 cut(s) 130, 204, 262, 269, 528
MunI CAATTG 1 cut(s) 713
MvaI CCWGG 4 cut(s) 130, 262, 269, 528
MwoI GCNNNNNNNGC 4 cut(s) 152, 176, 620, 958
NciI CCSGG 1 cut(s) 204
NdeII GATC 3 cut(s) 571, 660, 811
NlaIII CATG 3 cut(s) 506, 789, 943
NlaIV GGNNCC 7 cut(s) 200, 266, 300, 329, 330, 411, 573
NsiI ATGCAT 2 cut(s) 840, 847
NspI RCATGY 1 cut(s) 789
PagI TCATGA 1 cut(s) 939
PciI ACATGT 1 cut(s) 785
PdmI GAANNNNTTC 1 cut(s) 900
PflMI CCANNNNNTGG 1 cut(s) 881
PkrI GCNGC 5 cut(s) 148, 151, 154, 951, 954
PleI GAGTC 1 cut(s) 811
PpsI GAGTC 1 cut(s) 811
PpuMI RGGWCCY 1 cut(s) 328
PscI ACATGT 1 cut(s) 785
Psp5II RGGWCCY 1 cut(s) 328
Psp6I CCWGG 4 cut(s) 128, 260, 267, 526
PspGI CCWGG 4 cut(s) 128, 260, 267, 526
PspN4I GGNNCC 7 cut(s) 200, 266, 300, 329, 330, 411, 573
PspPI GGNCC 3 cut(s) 265, 328, 456
PspPPI RGGWCCY 1 cut(s) 328
PsuI RGATCY 1 cut(s) 571
PvuII CAGCTG 1 cut(s) 623
RsaI GTAC 2 cut(s) 200, 347
RsaNI GTAC 2 cut(s) 199, 346
RseI CAYNNNNRTG 1 cut(s) 944
SalI GTCGAC 1 cut(s) 401
SaqAI TTAA 3 cut(s) 11, 42, 552
SatI GCNGC 5 cut(s) 147, 150, 153, 950, 953
Sau3AI GATC 3 cut(s) 571, 660, 811
Sau96I GGNCC 3 cut(s) 265, 328, 456
SchI GAGTC 1 cut(s) 811
ScrFI CCNGG 5 cut(s) 130, 204, 262, 269, 528
SexAI ACCWGGT 1 cut(s) 128
SfaNI GCATC 3 cut(s) 174, 807, 854
SinI GGWCC 3 cut(s) 265, 328, 456
SmiMI CAYNNNNRTG 1 cut(s) 944
SmlI CTYRAG 4 cut(s) 349, 389, 551, 576
SmoI CTYRAG 4 cut(s) 349, 389, 551, 576
Sse9I AATT 6 cut(s) 18, 98, 683, 713, 737, 919
SsiI CCGC 3 cut(s) 146, 153, 179
SspI AATATT 1 cut(s) 934
StyD4I CCNGG 5 cut(s) 128, 202, 260, 267, 526
TaaI ACNGT 1 cut(s) 561
TaqI TCGA 2 cut(s) 402, 666
TasI AATT 6 cut(s) 18, 98, 683, 713, 737, 919
TauI GCSGC 2 cut(s) 149, 155
Tru1I TTAA 3 cut(s) 11, 42, 552
Tru9I TTAA 3 cut(s) 11, 42, 552
TseI GCWGC 3 cut(s) 149, 949, 952
TspDTI ATGAA 1 cut(s) 263
TspGWI ACGGA 2 cut(s) 94, 915
Van91I CCANNNNNTGG 1 cut(s) 881
Vha464I CTTAAG 1 cut(s) 551
VpaK11BI GGWCC 3 cut(s) 265, 328, 456
XapI RAATTY 3 cut(s) 98, 683, 919
XceI RCATGY 1 cut(s) 789
XmiI GTMKAC 1 cut(s) 402
XmnI GAANNNNTTC 1 cut(s) 900
Zsp2I ATGCAT 2 cut(s) 840, 847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.