MD15G1173000.v1.1

Heme oxygenase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005358
Physical Location & Seq
Forward (+)
13467187 .. 13470000
2814 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1173000.v1.1.491

Sequence Viewer

Length: 855 bp
ATGGCTTCTTTAACCCCGATTTCGCAATCCCAATCACTTTTCAACAAAAACCAGTTCAAGTTACCCCCAATTTTGAGCTCCGATTTCTTTCCGAAGAAGTTCTCTGTTTCGTTTCTGAGGATGAATTCCGGAGTGCCCACGAACGCCGCGGCGGCGGCGGCCGGGGTGGTTTCAGCCACCACGGCGGAGAAGGCGAAGAAGAGGTACCCCGGGGAGGCGAAGGGGTTCGTGGAGGAGATGAGGTTCGTGGCGATGAAGTTGCACACCAGGGACCAAGCTAAGGAGGGGGAGAAGGAAGTGAAGGAGCCGCAGGAGGGGCCGGTGGCTAAGTGGGAGCCAACGGTTGATGGGTATTTGAAGTTTTTGGTGGATAGCAAGTTGGTTTATGATACCCTTGAAGGGATTGTTGAAAAAGCCCCCTTTCCTTTTTACGCTGAGTTCAGAAATACTGGATTGGAAAGGTCTGAAAAGTTGGCGAAAGATTTGGAGTGGTTTAAGAAGCAAGGGTATGCCATACCAGAACCTTCTGCTCCGGGTGTAACCTACGCAGAGTATCTTAAGGAACTGTCGGAGAAGGATCCTCAAGCATTTATTTGCCACTTCTACAACATATACTTTGCTCATTCAGCTGGTGGTCGAATGATTGGGAAAAAGGTGGCGGAAATGATACTTGATAAAAAGGAATTGGAATTCTACAAATGGGACGGCGAGCTTCCCCAACTGTTGCAGAATGTACGGGAGAAGTTAAATAAAGTTGCGGAGACCTGGACGAGGGAGGAGAAGAACCACTGCTTGGAAGAAACCGAGAAATCGTTCAAACATTCGGGAGAGATTCTGCGTCTTATATTGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004392 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006629 GO:0006720 GO:0006721 GO:0006725 GO:0006778 GO:0006787 GO:0006788 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009058 GO:0009314 GO:0009411 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009648 GO:0009791 GO:0009812 GO:0009813 GO:0009888 GO:0009987 GO:0010019 GO:0010024 GO:0010073 GO:0010075 GO:0010119 GO:0010225 GO:0010228 GO:0010229 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0016491 GO:0016705 GO:0018130 GO:0019438 GO:0019439 GO:0020037 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0033013 GO:0033014 GO:0033015 GO:0034641 GO:0034644 GO:0040008 GO:0042168 GO:0042440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044270 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046148 GO:0046483 GO:0046700 GO:0046906 GO:0048037 GO:0048367 GO:0048507 GO:0048509 GO:0048573 GO:0048608 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051186 GO:0051187 GO:0051188 GO:0051202 GO:0051716 GO:0055114 GO:0061458 GO:0065007 GO:0071214 GO:0071478 GO:0071482 GO:0071494 GO:0071704 GO:0090567 GO:0097159 GO:0104004 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00860 ko01100 ko01110 map00860 map01100 map01110
Pfam Domains
Protein Families
Heme_oxygenase

Protein Analysis

285

Amino Acids

32.3

Weight (kDa)

7.68

Isoelectric Point (pI)

42.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Heme_oxygenase PF01126 95 - 278 2.6e-13 Heme oxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 204
AccB1I GGYRCC 1 cut(s) 204
AccB7I CCANNNNNTGG 1 cut(s) 793
AccII CGCG 1 cut(s) 149
AccIII TCCGGA 1 cut(s) 128
AciI CCGC 9 cut(s) 147, 149, 152, 155, 158, 185, 308, 659, 758
AclWI GGATC 2 cut(s) 572, 585
AcoI YGGCCR 1 cut(s) 159
AcsI RAATTY 2 cut(s) 124, 689
AfaI GTAC 2 cut(s) 206, 735
AfiI CCNNNNNNNGG 6 cut(s) 214, 280, 314, 399, 771, 793
AflII CTTAAG 1 cut(s) 557
AgsI TTSAA 6 cut(s) 43, 58, 358, 398, 410, 817
AjnI CCWGG 2 cut(s) 266, 764
AjuI GAANNNNNNNTTGG 2 cut(s) 776, 808
AloI GAACNNNNNNTCC 2 cut(s) 227, 259
AluBI AGCT 4 cut(s) 78, 278, 629, 712
AluI AGCT 4 cut(s) 78, 278, 629, 712
Alw21I GWGCWC 1 cut(s) 80
Alw26I GTCTC 1 cut(s) 755
AlwI GGATC 2 cut(s) 572, 585
Ama87I CYCGRG 1 cut(s) 209
Aor13HI TCCGGA 1 cut(s) 128
AoxI GGCC 2 cut(s) 159, 317
ApoI RAATTY 2 cut(s) 124, 689
Asp700I GAANNNNTTC 3 cut(s) 98, 224, 812
Asp718I GGTACC 1 cut(s) 204
AspS9I GGNCC 2 cut(s) 271, 317
AsuC2I CCSGG 4 cut(s) 163, 210, 211, 534
AvaI CYCGRG 1 cut(s) 209
AvaII GGWCC 1 cut(s) 271
BaeGI GKGCMC 1 cut(s) 138
BamHI GGATCC 1 cut(s) 577
BanI GGYRCC 1 cut(s) 204
BanII GRGCYC 1 cut(s) 80
BarI GAAGNNNNNNTAC 2 cut(s) 188, 220
Bbv12I GWGCWC 1 cut(s) 80
BccI CCATC 1 cut(s) 341
BceAI ACGGC 2 cut(s) 198, 721
BcgI CGANNNNNNTGC 2 cut(s) 241, 275
BciT130I CCWGG 2 cut(s) 268, 766
BcnI CCSGG 4 cut(s) 163, 210, 211, 534
BcoDI GTCTC 1 cut(s) 755
BfrI CTTAAG 1 cut(s) 557
BglI GCCNNNNNGGC 2 cut(s) 152, 182
BisI GCNGC 6 cut(s) 147, 150, 153, 156, 159, 308
BlsI GCNGC 6 cut(s) 148, 151, 154, 157, 160, 309
Bme1390I CCNGG 6 cut(s) 163, 210, 211, 268, 534, 766
Bme18I GGWCC 1 cut(s) 271
BmeT110I CYCGRG 1 cut(s) 209
BmgT120I GGNCC 2 cut(s) 271, 317
BmiI GGNNCC 6 cut(s) 206, 272, 306, 318, 336, 579
BmrFI CCNGG 6 cut(s) 163, 210, 211, 268, 534, 766
Bpu10I CCTNAGC 1 cut(s) 279
BpuEI CTTGAG 1 cut(s) 567
BpuMI CCSGG 4 cut(s) 163, 210, 211, 534
BsaI GGTCTC 1 cut(s) 755
BsaJI CCNNGG 7 cut(s) 147, 162, 180, 208, 209, 210, 267
BsaWI WCCGGW 1 cut(s) 128
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bsc4I CCNNNNNNNGG 6 cut(s) 214, 280, 314, 399, 771, 793
Bse118I RCCGGY 1 cut(s) 319
Bse1I ACTGG 2 cut(s) 52, 454
BseAI TCCGGA 1 cut(s) 128
BseBI CCWGG 2 cut(s) 268, 766
BseDI CCNNGG 7 cut(s) 147, 162, 180, 208, 209, 210, 267
BseGI GGATG 1 cut(s) 126
BseLI CCNNNNNNNGG 6 cut(s) 214, 280, 314, 399, 771, 793
BseMII CTCAG 2 cut(s) 107, 426
BseNI ACTGG 2 cut(s) 52, 454
BseRI GAGGAG 2 cut(s) 248, 791
BseSI GKGCMC 1 cut(s) 138
BseX3I CGGCCG 1 cut(s) 159
Bsh1236I CGCG 1 cut(s) 149
Bsh1285I CGRYCG 1 cut(s) 162
BshFI GGCC 2 cut(s) 161, 319
BshNI GGYRCC 1 cut(s) 204
BsiEI CGRYCG 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 80
BsiHKCI CYCGRG 1 cut(s) 209
BsiSI CCGG 5 cut(s) 129, 162, 210, 320, 533
BslFI GGGAC 2 cut(s) 284, 716
BslI CCNNNNNNNGG 6 cut(s) 214, 280, 314, 399, 771, 793
BsmAI GTCTC 1 cut(s) 755
BsmFI GGGAC 2 cut(s) 284, 716
BsnI GGCC 2 cut(s) 161, 319
Bso31I GGTCTC 1 cut(s) 755
BsoBI CYCGRG 1 cut(s) 209
Bsp1286I GDGCHC 2 cut(s) 80, 138
Bsp13I TCCGGA 1 cut(s) 128
Bsp143I GATC 1 cut(s) 577
BspACI CCGC 9 cut(s) 147, 149, 152, 155, 158, 185, 308, 659, 758
BspANI GGCC 2 cut(s) 161, 319
BspCNI CTCAG 2 cut(s) 108, 427
BspEI TCCGGA 1 cut(s) 128
BspFNI CGCG 1 cut(s) 149
BspHI TCATGA 1 cut(s) 851
BspLI GGNNCC 6 cut(s) 206, 272, 306, 318, 336, 579
BspPI GGATC 2 cut(s) 572, 585
BspT107I GGYRCC 1 cut(s) 204
BspTI CTTAAG 1 cut(s) 557
BspTNI GGTCTC 1 cut(s) 755
BsrFI RCCGGY 1 cut(s) 319
BsrI ACTGG 2 cut(s) 52, 454
BssAI RCCGGY 1 cut(s) 319
BssECI CCNNGG 7 cut(s) 147, 162, 180, 208, 209, 210, 267
BssMI GATC 1 cut(s) 577
Bst2UI CCWGG 2 cut(s) 268, 766
Bst4CI ACNGT 3 cut(s) 343, 567, 723
Bst6I CTCTTC 1 cut(s) 194
BstAFI CTTAAG 1 cut(s) 557
BstC8I GCNNGC 1 cut(s) 710
BstDEI CTNAG 4 cut(s) 116, 279, 327, 435
BstDSI CCRYGG 2 cut(s) 147, 180
BstENI CCTNNNNNAGG 1 cut(s) 769
BstF5I GGATG 1 cut(s) 126
BstFNI CGCG 1 cut(s) 149
BstKTI GATC 1 cut(s) 580
BstMAI GTCTC 1 cut(s) 755
BstMBI GATC 1 cut(s) 577
BstMCI CGRYCG 1 cut(s) 162
BstMWI GCNNNNNNNGC 7 cut(s) 152, 155, 158, 182, 191, 316, 626
BstNI CCWGG 2 cut(s) 268, 766
BstSCI CCNGG 6 cut(s) 161, 208, 209, 266, 532, 764
BstSLI GKGCMC 1 cut(s) 138
BstUI CGCG 1 cut(s) 149
BstX2I RGATCY 1 cut(s) 577
BstYI RGATCY 1 cut(s) 577
BstZI CGGCCG 1 cut(s) 159
BsuRI GGCC 2 cut(s) 161, 319
BtgI CCRYGG 2 cut(s) 147, 180
BtgZI GCGATG 1 cut(s) 266
BtsCI GGATG 1 cut(s) 126
BtsI GCAGTG 1 cut(s) 787
BtsIMutI CAGTG 1 cut(s) 787
Cac8I GCNNGC 1 cut(s) 710
CciI TCATGA 1 cut(s) 851
Cfr10I RCCGGY 1 cut(s) 319
Cfr13I GGNCC 2 cut(s) 271, 317
Cfr42I CCGCGG 1 cut(s) 150
Cfr9I CCCGGG 1 cut(s) 209
CseI GACGC 1 cut(s) 827
Csp6I GTAC 2 cut(s) 205, 734
CviAII CATG 1 cut(s) 852
CviQI GTAC 2 cut(s) 205, 734
DdeI CTNAG 4 cut(s) 116, 279, 327, 435
DpnI GATC 1 cut(s) 579
DpnII GATC 1 cut(s) 577
EaeI YGGCCR 1 cut(s) 159
EagI CGGCCG 1 cut(s) 159
Eam1104I CTCTTC 1 cut(s) 194
EarI CTCTTC 1 cut(s) 194
EciI GGCGGA 2 cut(s) 200, 674
Ecl136II GAGCTC 1 cut(s) 78
EclXI CGGCCG 1 cut(s) 159
Eco24I GRGCYC 1 cut(s) 80
Eco31I GGTCTC 1 cut(s) 755
Eco47I GGWCC 1 cut(s) 271
Eco52I CGGCCG 1 cut(s) 159
Eco53kI GAGCTC 1 cut(s) 78
Eco88I CYCGRG 1 cut(s) 209
EcoICRI GAGCTC 1 cut(s) 78
EcoNI CCTNNNNNAGG 1 cut(s) 769
EcoRI GAATTC 2 cut(s) 124, 689
EcoRII CCWGG 2 cut(s) 266, 764
EcoT38I GRGCYC 1 cut(s) 80
FaeI CATG 1 cut(s) 855
FaiI YATR 7 cut(s) 387, 510, 515, 611, 613, 845, 853
FaqI GGGAC 2 cut(s) 284, 716
FatI CATG 1 cut(s) 851
Fnu4HI GCNGC 6 cut(s) 147, 150, 153, 156, 159, 308
FokI GGATG 1 cut(s) 133
FriOI GRGCYC 1 cut(s) 80
Fsp4HI GCNGC 6 cut(s) 147, 150, 153, 156, 159, 308
GluI GCNGC 6 cut(s) 147, 150, 153, 156, 159, 308
HaeIII GGCC 2 cut(s) 161, 319
HapII CCGG 5 cut(s) 129, 162, 210, 320, 533
HgaI GACGC 1 cut(s) 827
Hin1II CATG 1 cut(s) 855
HinfI GANTC 1 cut(s) 832
HpaII CCGG 5 cut(s) 129, 162, 210, 320, 533
Hpy188I TCNGA 6 cut(s) 82, 93, 117, 443, 466, 571
Hpy188III TCNNGA 3 cut(s) 129, 825, 852
HpyAV CCTTC 7 cut(s) 184, 214, 286, 295, 392, 534, 568
HpyCH4III ACNGT 3 cut(s) 343, 567, 723
HpyCH4V TGCA 2 cut(s) 262, 727
HpyF10VI GCNNNNNNNGC 7 cut(s) 152, 155, 158, 182, 191, 316, 626
HpyF3I CTNAG 4 cut(s) 116, 279, 327, 435
Hsp92II CATG 1 cut(s) 855
Kpn2I TCCGGA 1 cut(s) 128
KpnI GGTACC 1 cut(s) 208
KspI CCGCGG 1 cut(s) 150
Kzo9I GATC 1 cut(s) 577
LmnI GCTCC 4 cut(s) 83, 304, 334, 535
MaeIII GTNAC 2 cut(s) 60, 538
MalI GATC 1 cut(s) 579
MboI GATC 1 cut(s) 577
MboII GAAGA 5 cut(s) 106, 208, 211, 793, 809
MflI RGATCY 1 cut(s) 577
MhlI GDGCHC 2 cut(s) 80, 138
MluCI AATT 4 cut(s) 69, 124, 683, 689
MmeI TCCRAC 1 cut(s) 549
MroI TCCGGA 1 cut(s) 128
MroXI GAANNNNTTC 3 cut(s) 98, 224, 812
MseI TTAA 4 cut(s) 11, 495, 558, 746
MspA1I CMGCKG 2 cut(s) 149, 629
MspCI CTTAAG 1 cut(s) 557
MspI CCGG 5 cut(s) 129, 162, 210, 320, 533
MspR9I CCNGG 6 cut(s) 163, 210, 211, 268, 534, 766
MvaI CCWGG 2 cut(s) 268, 766
MvnI CGCG 1 cut(s) 149
MwoI GCNNNNNNNGC 7 cut(s) 152, 155, 158, 182, 191, 316, 626
NciI CCSGG 4 cut(s) 163, 210, 211, 534
NdeII GATC 1 cut(s) 577
NlaIII CATG 1 cut(s) 855
NlaIV GGNNCC 6 cut(s) 206, 272, 306, 318, 336, 579
PagI TCATGA 1 cut(s) 851
PdmI GAANNNNTTC 3 cut(s) 98, 224, 812
PfeI GAWTC 1 cut(s) 832
PflMI CCANNNNNTGG 1 cut(s) 793
PkrI GCNGC 6 cut(s) 148, 151, 154, 157, 160, 309
Psp124BI GAGCTC 1 cut(s) 80
Psp6I CCWGG 2 cut(s) 266, 764
PspGI CCWGG 2 cut(s) 266, 764
PspN4I GGNNCC 6 cut(s) 206, 272, 306, 318, 336, 579
PspPI GGNCC 2 cut(s) 271, 317
PsuI RGATCY 1 cut(s) 577
PvuII CAGCTG 1 cut(s) 629
RsaI GTAC 2 cut(s) 206, 735
RsaNI GTAC 2 cut(s) 205, 734
SacI GAGCTC 1 cut(s) 80
SacII CCGCGG 1 cut(s) 150
SaqAI TTAA 4 cut(s) 11, 495, 558, 746
SatI GCNGC 6 cut(s) 147, 150, 153, 156, 159, 308
Sau3AI GATC 1 cut(s) 577
Sau96I GGNCC 2 cut(s) 271, 317
ScrFI CCNGG 6 cut(s) 163, 210, 211, 268, 534, 766
SduI GDGCHC 2 cut(s) 80, 138
Sfr303I CCGCGG 1 cut(s) 150
SgrBI CCGCGG 1 cut(s) 150
SinI GGWCC 1 cut(s) 271
SmaI CCCGGG 1 cut(s) 211
SmlI CTYRAG 2 cut(s) 557, 582
SmoI CTYRAG 2 cut(s) 557, 582
Sse9I AATT 4 cut(s) 69, 124, 683, 689
SsiI CCGC 9 cut(s) 147, 149, 152, 155, 158, 185, 308, 659, 758
SstI GAGCTC 1 cut(s) 80
StyD4I CCNGG 6 cut(s) 161, 208, 209, 266, 532, 764
TaaI ACNGT 3 cut(s) 343, 567, 723
TaqI TCGA 1 cut(s) 637
TasI AATT 4 cut(s) 69, 124, 683, 689
TauI GCSGC 6 cut(s) 149, 152, 155, 158, 161, 310
TfiI GAWTC 1 cut(s) 832
Tru1I TTAA 4 cut(s) 11, 495, 558, 746
Tru9I TTAA 4 cut(s) 11, 495, 558, 746
TscAI CASTG 1 cut(s) 794
TspDTI ATGAA 2 cut(s) 137, 269
TspMI CCCGGG 1 cut(s) 209
TspRI CASTG 1 cut(s) 794
Van91I CCANNNNNTGG 1 cut(s) 793
Vha464I CTTAAG 1 cut(s) 557
VpaK11BI GGWCC 1 cut(s) 271
XagI CCTNNNNNAGG 1 cut(s) 769
XapI RAATTY 2 cut(s) 124, 689
XmaI CCCGGG 1 cut(s) 209
XmnI GAANNNNTTC 3 cut(s) 98, 224, 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.