Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0002195

Physical Location & Seq

Genomic Coordinates Forward (+)

7675438 .. 7681328

Gene Structure 5891 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1097200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1692 bp

ATGGCTGCCTCCTCTGTTGCATCTCCGCTCTGCACGTGGCTGGTGGCTGCTTGCATGTCGGTCACGTGCGACAGGGACTGCTCCCCCAGGGTATCTATGCTCAGCTCCTCCCGGAGGCGGCCGAAATGCGCCGGAAGAAGGGGGCTGCCGTCCAAATGCAGTAGCTTTAGTGGCGAATTTCAGAAGGGTCTGATTTCTGCGTTCAGTGGATCCAGCATTCAGGGCCTCATGAGCTCCTGCTTGGCTTTCGAGCCGTGCGATGAGTACTACAGCTCAAAGGGTTATTCTTCTTTGGGATGCAGCGGGCTCTCGTCACTGTTTGGATCCAAATCTGGGGCTTCCAATCGAAGGCAGAGGTGTTTTAATGGAGCCGCATATTCTGGGGAAACCATGGCTGTAGCTGTGCAACCTACTGAAGAACTAACAAAACAGAAGAAACCTTTAACAAGGCAGAGGCGGGTGGTTGTGACAGGAATGAGTGTGGTGACCCCACTCGGACATGATTCTGATGTCTTCTACAATAATTTGCTTGAGGGAGTTAGTGGCATTAGTGAGATAGAAGCTTTTGATTGTACCCAGTTTCCAACGAGGATTGGTGGTGAGATCAAGTCTTTCTCAACGGACGGATGGGTAGCACCAAAGTTTTCTAGAAGAATGGATAAGTTCATGCTTTATTTGCTTACTGCTGGAAAAAAAGCCTTGGCAGATGCTGGAATCACAGAAGAAGTCATGGAGACGCTAGACAAAGCAAAATGTGGGGTTTTGATTGGCTCAGCAATGGGTGGCATGAAGATCTTCAATGATGCAATTGAAGCTTTACGGGTATCCTATAAGAAGATGAACCCTTTCTGTGTACCTTTCGCAACCACAAATATGGGTTCTGCCATGCTGGCAATGGATCTGGGTTGGATGGGCCCAAATTATTCAATTTCTACTGCTTGTGCTACAAGCAACTTCTGTATTTTGAGTTCGGCTAATCATATTATTAGAGGTGAAGCAGACCTTATGCTTTGTGGTGGTTCAGATTCAGTGATGATACCTATTGGATTGGGAGGCTTTGTGGCATGCAGAGCACTGTCACAAAGAAACAATGATCCAACCAAAGCATCACGCCCTTGGGATAGTAATCGTGATGGCTTTGTTATGGGGGAAGGAGCTGGCGTTTTGCTTCTAGAAGAGTTAGAACATGCTAAGAAAAGAGGTGCAGCTATTTATGCAGAATTCCTTGGTGGAAGCTTCGCATGTGATGCTTACCACATGACTGAACCACACCCTGATGGGGCTGGGGTTATTCTCTGCATTGAAAAGGCTTTAGAACAGTCTGGGGTGTCTAGGGAAGATGTTAATTACATTAATGCACATGCTACATCCACACCGGCCGGAGATCTCAAGGAGTATAATGCTCTCATTCATTGTTTTGGCCAGAATCCTGAGTTGAGAGTGAACTCTACAAAATCCATGATTGGTCACCTACTGGGAGCATCTGGTGCTGTAGAAGCTGTTGCAGCAGTACAGGCAATAAGAACTGGGTGGATACATCCAAATATTAATCTGGAAAACCCAGATGAGGGCATGGATACAAAACTACTAGTGGGTCCAAAGAAAGAGAGACTGGACGTTAAGGTGGCATTGTCTAACTCATTTGGGTTCGGTGGCCACAACTCATCGATCTTGTTTGCCCCATACAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004312 GO:0004315 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006636 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009266 GO:0009409 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009631 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016053 GO:0016740 GO:0016746 GO:0016747 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0032787 GO:0033559 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0071704 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00061 ko00780 ko01100 ko01212 map00061 map00780 map01100 map01212

Pfam Domains

Protein Families

Ketoacyl-synt_C ketoacyl-synt

Protein Analysis

564

Amino Acids

60.16

Weight (kDa)

7.76

Isoelectric Point (pI)

48.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ketoacyl-synt	PF00109	152 - 397	2.5e-58	Beta-ketoacyl synthase, N-terminal domain
Ketoacyl-synt_C	PF02801	405 - 519	7.1e-35	Beta-ketoacyl synthase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002195)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74960 AT1G74960 AT1G74960
fragaria_vesca	FvH4_1g09200 FvH4_1g09200 FvH4_1g09200 FvH4_1g09200 FvH4_6g35220 FvH4_6g35220
malus_domestica	MD02G1097200.v1.1 MD02G1097500.v1.1 MD02G1098500.v1.1 MD02G1101500.v1.1 MD09G1177500.v1.1 MD15G1221800.v1.1
prunus_persica	Prupe.3G036900_v2.0.a1 Prupe.3G036900_v2.0.a1 Prupe.7G193900_v2.0.a1 Prupe.7G193900_v2.0.a1 Prupe.7G193900_v2.0.a1
pyrus_communis	pycom02g07700 pycom15g19630
rosa_chinensis	RchiOBHm_Chr2g0095651 RchiOBHm_Chr2g0146561
rosa_laevigata	RLG00000016584 RLG00000020207
rosa_multiflora	Rmu_sc0001309.1_g000017 Rmu_sc0001986.1_g000028
rosa_roxburghii	Rroxscaffold_2G00100040 Rroxscaffold_2G00146150
rosa_rugosa	Rorug02G0052800 Rorug02G0394700 Rorug02G0394800 Rorug02G0394800
rosa_samantha	Rh2AG100800 Rh2AG451600 Rh2BG464400 Rh2CG104800 Rh2CG438500 Rh2DG102000 Rh2DG473400
rosa_wichuraiana	Rw2G007650 Rw2G036890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	28
AciI	CCGC	5 cut(s)	26, 118, 303, 372, 457
AclWI	GGATC	6 cut(s)	204, 217, 318, 331, 906, 1088
AcoI	YGGCCR	4 cut(s)	119, 1377, 1420, 1654
AcsI	RAATTY	2 cut(s)	176, 1220
AcuI	CTGAAG	1 cut(s)	435
AcvI	CACGTG	2 cut(s)	36, 66
AfaI	GTAC	4 cut(s)	266, 574, 855, 1512
AfiI	CCNNNNNNNGG	8 cut(s)	114, 117, 138, 333, 348, 1279, 1567, 1568
AgsI	TTSAA	4 cut(s)	799, 812, 927, 1304
AhlI	ACTAGT	1 cut(s)	1588
AjnI	CCWGG	1 cut(s)	86
Alw21I	GWGCWC	2 cut(s)	236, 1075
Alw26I	GTCTC	2 cut(s)	728, 1603
AlwI	GGATC	6 cut(s)	204, 217, 318, 331, 906, 1088
AlwNI	CAGNNNCTG	2 cut(s)	78, 710
AoxI	GGCC	6 cut(s)	119, 223, 913, 1377, 1420, 1654
ApaI	GGGCCC	1 cut(s)	917
ApeKI	GCWGC	6 cut(s)	5, 47, 145, 300, 1205, 1505
ApoI	RAATTY	2 cut(s)	176, 1220
AseI	ATTAAT	2 cut(s)	1353, 1548
Asp700I	GAANNNNTTC	2 cut(s)	794, 845
AspLEI	GCGC	1 cut(s)	131
AspS9I	GGNCC	4 cut(s)	223, 913, 914, 1595
AsuC2I	CCSGG	1 cut(s)	112
AsuHPI	GGTGA	4 cut(s)	496, 611, 1004, 1460
AvaII	GGWCC	1 cut(s)	1595
BaeGI	GKGCMC	1 cut(s)	917
BalI	TGGCCA	2 cut(s)	1422, 1656
BamHI	GGATCC	2 cut(s)	209, 323
BanII	GRGCYC	3 cut(s)	236, 309, 917
BbrPI	CACGTG	2 cut(s)	36, 66
BbsI	GAAGAC	1 cut(s)	505
Bbv12I	GWGCWC	2 cut(s)	236, 1075
BbvI	GCAGC	5 cut(s)	34, 132, 312, 1217, 1517
BccI	CCATC	4 cut(s)	621, 904, 1127, 1271
BceAI	ACGGC	2 cut(s)	133, 238
BciT130I	CCWGG	1 cut(s)	88
BciVI	GTATCC	3 cut(s)	835, 1527, 1570
BcnI	CCSGG	1 cut(s)	112
BcoDI	GTCTC	2 cut(s)	728, 1603
BcuI	ACTAGT	1 cut(s)	1588
BfaI	CTAG	5 cut(s)	648, 740, 1172, 1332, 1589
BfmI	CTRYAG	3 cut(s)	268, 396, 1491
BfuI	GTATCC	3 cut(s)	835, 1527, 1570
BglI	GCCNNNNNGGC	1 cut(s)	890
BglII	AGATCT	2 cut(s)	792, 1384
BisI	GCNGC	8 cut(s)	6, 48, 119, 146, 301, 372, 1206, 1506
BlpI	GCTNAGC	2 cut(s)	101, 772
BlsI	GCNGC	8 cut(s)	7, 49, 120, 147, 302, 373, 1207, 1507
BmcAI	AGTACT	1 cut(s)	266
Bme1390I	CCNGG	2 cut(s)	88, 112
Bme18I	GGWCC	1 cut(s)	1595
BmgT120I	GGNCC	4 cut(s)	223, 913, 914, 1595
BmiI	GGNNCC	5 cut(s)	211, 325, 370, 915, 1596
BmrFI	CCNGG	2 cut(s)	88, 112
BmrI	ACTGGG	3 cut(s)	571, 1484, 1536
BmsI	GCATC	7 cut(s)	29, 287, 697, 793, 1115, 1237, 1490
BmuI	ACTGGG	3 cut(s)	571, 1484, 1536
BpiI	GAAGAC	1 cut(s)	505
Bpu1102I	GCTNAGC	2 cut(s)	101, 772
BpuEI	CTTGAG	2 cut(s)	551, 1373
BpuMI	CCSGG	1 cut(s)	112
Bsa29I	ATCGAT	1 cut(s)	1667
BsaAI	YACGTR	2 cut(s)	36, 66
BsaBI	GATNNNNATC	2 cut(s)	328, 1125
BsaJI	CCNNGG	6 cut(s)	86, 87, 390, 699, 1115, 1225
BsaXI	ACNNNNNCTCC	4 cut(s)	1044, 1074, 1146, 1176
Bsc4I	CCNNNNNNNGG	8 cut(s)	114, 117, 138, 333, 348, 1279, 1567, 1568
Bse118I	RCCGGY	1 cut(s)	1375
Bse1I	ACTGG	4 cut(s)	577, 1479, 1531, 1617
Bse3DI	GCAATG	2 cut(s)	783, 900
Bse8I	GATNNNNATC	2 cut(s)	328, 1125
BseBI	CCWGG	1 cut(s)	88
BseCI	ATCGAT	1 cut(s)	1667
BseDI	CCNNGG	6 cut(s)	86, 87, 390, 699, 1115, 1225
BseGI	GGATG	5 cut(s)	302, 632, 915, 1367, 1537
BseJI	GATNNNNATC	2 cut(s)	328, 1125
BseLI	CCNNNNNNNGG	8 cut(s)	114, 117, 138, 333, 348, 1279, 1567, 1568
BseMI	GCAATG	2 cut(s)	783, 900
BseMII	CTCAG	3 cut(s)	115, 786, 1422
BseNI	ACTGG	4 cut(s)	577, 1479, 1531, 1617
BseRI	GAGGAG	1 cut(s)	97
BseSI	GKGCMC	1 cut(s)	917
BseX3I	CGGCCG	2 cut(s)	119, 1377
BseXI	GCAGC	5 cut(s)	34, 132, 312, 1217, 1517
BseYI	CCCAGC	1 cut(s)	1283
BsgI	GTGCAG	2 cut(s)	16, 1224
Bsh1285I	CGRYCG	2 cut(s)	122, 1380
BshFI	GGCC	6 cut(s)	121, 225, 915, 1379, 1422, 1656
BshVI	ATCGAT	1 cut(s)	1667
BsiEI	CGRYCG	2 cut(s)	122, 1380
BsiHKAI	GWGCWC	2 cut(s)	236, 1075
BsiSI	CCGG	4 cut(s)	112, 132, 1376, 1380
BslFI	GGGAC	1 cut(s)	89
BslI	CCNNNNNNNGG	8 cut(s)	114, 117, 138, 333, 348, 1279, 1567, 1568
BsmAI	GTCTC	2 cut(s)	728, 1603
BsmBI	CGTCTC	1 cut(s)	728
BsmFI	GGGAC	1 cut(s)	89
BsmI	GAATGC	1 cut(s)	216
BsnI	GGCC	6 cut(s)	121, 225, 915, 1379, 1422, 1656
Bsp120I	GGGCCC	1 cut(s)	913
Bsp1286I	GDGCHC	4 cut(s)	236, 309, 917, 1075
Bsp143I	GATC	8 cut(s)	209, 323, 603, 792, 898, 1093, 1384, 1668
Bsp1720I	GCTNAGC	2 cut(s)	101, 772
Bsp19I	CCATGG	1 cut(s)	390
BspACI	CCGC	5 cut(s)	26, 118, 303, 372, 457
BspANI	GGCC	6 cut(s)	121, 225, 915, 1379, 1422, 1656
BspCNI	CTCAG	3 cut(s)	114, 785, 1423
BspDI	ATCGAT	1 cut(s)	1667
BspHI	TCATGA	1 cut(s)	228
BspLI	GGNNCC	5 cut(s)	211, 325, 370, 915, 1596
BspPI	GGATC	6 cut(s)	204, 217, 318, 331, 906, 1088
BsrBI	CCGCTC	1 cut(s)	28
BsrDI	GCAATG	2 cut(s)	783, 900
BsrFI	RCCGGY	1 cut(s)	1375
BsrI	ACTGG	4 cut(s)	577, 1479, 1531, 1617
BssAI	RCCGGY	1 cut(s)	1375
BssECI	CCNNGG	6 cut(s)	86, 87, 390, 699, 1115, 1225
BssMI	GATC	8 cut(s)	209, 323, 603, 792, 898, 1093, 1384, 1668
BssT1I	CCWWGG	4 cut(s)	390, 699, 1115, 1225
Bst2UI	CCWGG	1 cut(s)	88
Bst4CI	ACNGT	3 cut(s)	318, 1077, 1320
Bst6I	CTCTTC	1 cut(s)	1170
BstAPI	GCANNNNNTGC	2 cut(s)	1247, 1487
BstBAI	YACGTR	2 cut(s)	36, 66
BstC8I	GCNNGC	5 cut(s)	52, 305, 891, 1066, 1159
BstDEI	CTNAG	4 cut(s)	101, 772, 1191, 1431
BstDSI	CCRYGG	1 cut(s)	390
BstEII	GGTNACC	2 cut(s)	484, 1466
BstENI	CCTNNNNNAGG	1 cut(s)	112
BstF5I	GGATG	5 cut(s)	302, 632, 915, 1367, 1537
BstHHI	GCGC	1 cut(s)	131
BstKTI	GATC	8 cut(s)	212, 326, 606, 795, 901, 1096, 1387, 1671
BstMAI	GTCTC	2 cut(s)	728, 1603
BstMBI	GATC	8 cut(s)	209, 323, 603, 792, 898, 1093, 1384, 1668
BstMCI	CGRYCG	2 cut(s)	122, 1380
BstNI	CCWGG	1 cut(s)	88
BstNSI	RCATGY	5 cut(s)	58, 1068, 1190, 1245, 1364
BstPI	GGTNACC	2 cut(s)	484, 1466
BstSCI	CCNGG	2 cut(s)	86, 110
BstSFI	CTRYAG	3 cut(s)	268, 396, 1491
BstSLI	GKGCMC	1 cut(s)	917
BstV1I	GCAGC	5 cut(s)	34, 132, 312, 1217, 1517
BstV2I	GAAGAC	1 cut(s)	505
BstX2I	RGATCY	5 cut(s)	209, 323, 792, 898, 1384
BstXI	CCANNNNNNTGG	1 cut(s)	874
BstYI	RGATCY	5 cut(s)	209, 323, 792, 898, 1384
BstZI	CGGCCG	2 cut(s)	119, 1377
Bsu15I	ATCGAT	1 cut(s)	1667
BsuI	GTATCC	3 cut(s)	835, 1527, 1570
BsuRI	GGCC	6 cut(s)	121, 225, 915, 1379, 1422, 1656
BsuTUI	ATCGAT	1 cut(s)	1667
BtgI	CCRYGG	1 cut(s)	390
BtgZI	GCGATG	1 cut(s)	273
BtsCI	GGATG	5 cut(s)	302, 632, 915, 1367, 1537
BtsIMutI	CAGTG	4 cut(s)	211, 314, 1035, 1073
Cac8I	GCNNGC	5 cut(s)	52, 305, 891, 1066, 1159
CaiI	CAGNNNCTG	2 cut(s)	78, 710
CciI	TCATGA	1 cut(s)	228
CfoI	GCGC	1 cut(s)	131
Cfr10I	RCCGGY	1 cut(s)	1375
Cfr13I	GGNCC	4 cut(s)	223, 913, 914, 1595
ClaI	ATCGAT	1 cut(s)	1667
CseI	GACGC	1 cut(s)	745
Csp6I	GTAC	4 cut(s)	265, 573, 854, 1511
CviQI	GTAC	4 cut(s)	265, 573, 854, 1511
DdeI	CTNAG	4 cut(s)	101, 772, 1191, 1431
DpnI	GATC	8 cut(s)	211, 325, 605, 794, 900, 1095, 1386, 1670
DpnII	GATC	8 cut(s)	209, 323, 603, 792, 898, 1093, 1384, 1668
EaeI	YGGCCR	4 cut(s)	119, 1377, 1420, 1654
EagI	CGGCCG	2 cut(s)	119, 1377
Eam1104I	CTCTTC	1 cut(s)	1170
EarI	CTCTTC	1 cut(s)	1170
Ecl136II	GAGCTC	1 cut(s)	234
EclXI	CGGCCG	2 cut(s)	119, 1377
Eco130I	CCWWGG	4 cut(s)	390, 699, 1115, 1225
Eco24I	GRGCYC	3 cut(s)	236, 309, 917
Eco47I	GGWCC	1 cut(s)	1595
Eco52I	CGGCCG	2 cut(s)	119, 1377
Eco53kI	GAGCTC	1 cut(s)	234
Eco57I	CTGAAG	1 cut(s)	435
Eco72I	CACGTG	2 cut(s)	36, 66
Eco91I	GGTNACC	2 cut(s)	484, 1466
EcoICRI	GAGCTC	1 cut(s)	234
EcoNI	CCTNNNNNAGG	1 cut(s)	112
EcoO109I	RGGNCCY	1 cut(s)	223
EcoO65I	GGTNACC	2 cut(s)	484, 1466
EcoRI	GAATTC	1 cut(s)	1220
EcoRII	CCWGG	1 cut(s)	86
EcoT14I	CCWWGG	4 cut(s)	390, 699, 1115, 1225
EcoT38I	GRGCYC	3 cut(s)	236, 309, 917
ErhI	CCWWGG	4 cut(s)	390, 699, 1115, 1225
Esp3I	CGTCTC	1 cut(s)	728
FalI	AAGNNNNNCTT	2 cut(s)	987, 1019
FaqI	GGGAC	1 cut(s)	89
FauI	CCCGC	2 cut(s)	296, 450
Fnu4HI	GCNGC	8 cut(s)	6, 48, 119, 146, 301, 372, 1206, 1506
FokI	GGATG	5 cut(s)	309, 639, 922, 1354, 1524
FriOI	GRGCYC	3 cut(s)	236, 309, 917
Fsp4HI	GCNGC	8 cut(s)	6, 48, 119, 146, 301, 372, 1206, 1506
FspBI	CTAG	5 cut(s)	648, 740, 1172, 1332, 1589
GlaI	GCGC	1 cut(s)	130
GluI	GCNGC	8 cut(s)	6, 48, 119, 146, 301, 372, 1206, 1506
GsaI	CCCAGC	1 cut(s)	1287
HaeIII	GGCC	6 cut(s)	121, 225, 915, 1379, 1422, 1656
HapII	CCGG	4 cut(s)	112, 132, 1376, 1380
HgaI	GACGC	1 cut(s)	745
HhaI	GCGC	1 cut(s)	131
Hin6I	GCGC	1 cut(s)	129
HinP1I	GCGC	1 cut(s)	129
HindIII	AAGCTT	3 cut(s)	561, 813, 1234
HinfI	GANTC	4 cut(s)	503, 714, 1025, 1426
HpaII	CCGG	4 cut(s)	112, 132, 1376, 1380
HphI	GGTGA	4 cut(s)	496, 611, 1004, 1460
Hpy166II	GTNNAC	2 cut(s)	854, 1444
Hpy188I	TCNGA	5 cut(s)	183, 192, 497, 508, 1024
Hpy188III	TCNNGA	6 cut(s)	229, 648, 1130, 1172, 1430, 1553
Hpy8I	GTNNAC	2 cut(s)	854, 1444
HpyAV	CCTTC	4 cut(s)	132, 178, 342, 1145
HpyCH4III	ACNGT	3 cut(s)	318, 1077, 1320
HpyCH4IV	ACGT	3 cut(s)	35, 65, 1617
HpyF3I	CTNAG	4 cut(s)	101, 772, 1191, 1431
HpySE526I	ACGT	3 cut(s)	35, 65, 1617
HspAI	GCGC	1 cut(s)	129
Kzo9I	GATC	8 cut(s)	209, 323, 603, 792, 898, 1093, 1384, 1668
LmnI	GCTCC	6 cut(s)	86, 110, 239, 368, 1154, 1478
Lsp1109I	GCAGC	5 cut(s)	34, 132, 312, 1217, 1517
LweI	GCATC	7 cut(s)	29, 287, 697, 793, 1115, 1237, 1490
MaeI	CTAG	5 cut(s)	648, 740, 1172, 1332, 1589
MaeII	ACGT	3 cut(s)	35, 65, 1617
MaeIII	GTNAC	6 cut(s)	61, 312, 466, 484, 1077, 1466
MalI	GATC	8 cut(s)	211, 325, 605, 794, 900, 1095, 1386, 1670
MbiI	CCGCTC	1 cut(s)	28
MboI	GATC	8 cut(s)	209, 323, 603, 792, 898, 1093, 1384, 1668
MfeI	CAATTG	1 cut(s)	807
MflI	RGATCY	5 cut(s)	209, 323, 792, 898, 1384
MhlI	GDGCHC	4 cut(s)	236, 309, 917, 1075
MlsI	TGGCCA	2 cut(s)	1422, 1656
MluCI	AATT	7 cut(s)	176, 523, 807, 919, 927, 1220, 1345
MluNI	TGGCCA	2 cut(s)	1422, 1656
MmeI	TCCRAC	3 cut(s)	608, 887, 1121
Mox20I	TGGCCA	2 cut(s)	1422, 1656
MroXI	GAANNNNTTC	2 cut(s)	794, 845
MscI	TGGCCA	2 cut(s)	1422, 1656
MseI	TTAA	6 cut(s)	363, 443, 1344, 1353, 1548, 1620
MslI	CAYNNNNRTG	2 cut(s)	872, 1275
Msp20I	TGGCCA	2 cut(s)	1422, 1656
MspA1I	CMGCKG	1 cut(s)	303
MspI	CCGG	4 cut(s)	112, 132, 1376, 1380
MspR9I	CCNGG	2 cut(s)	88, 112
MunI	CAATTG	1 cut(s)	807
Mva1269I	GAATGC	1 cut(s)	216
MvaI	CCWGG	1 cut(s)	88
NciI	CCSGG	1 cut(s)	112
NcoI	CCATGG	1 cut(s)	390
NdeII	GATC	8 cut(s)	209, 323, 603, 792, 898, 1093, 1384, 1668
NlaIV	GGNNCC	5 cut(s)	211, 325, 370, 915, 1596
NmuCI	GTSAC	6 cut(s)	61, 312, 466, 484, 1077, 1466
NspI	RCATGY	5 cut(s)	58, 1068, 1190, 1245, 1364
PaeI	GCATGC	1 cut(s)	1068
PagI	TCATGA	1 cut(s)	228
PasI	CCCWGGG	1 cut(s)	87
PcsI	WCGNNNNNNNCGW	1 cut(s)	255
PctI	GAATGC	1 cut(s)	216
PdmI	GAANNNNTTC	2 cut(s)	794, 845
PfeI	GAWTC	4 cut(s)	503, 714, 1025, 1426
PfoI	TCCNGGA	1 cut(s)	110
PkrI	GCNGC	8 cut(s)	7, 49, 120, 147, 302, 373, 1207, 1507
PmaCI	CACGTG	2 cut(s)	36, 66
PmlI	CACGTG	2 cut(s)	36, 66
Ppu21I	YACGTR	2 cut(s)	36, 66
PshBI	ATTAAT	2 cut(s)	1353, 1548
Psp124BI	GAGCTC	1 cut(s)	236
Psp6I	CCWGG	1 cut(s)	86
PspCI	CACGTG	2 cut(s)	36, 66
PspEI	GGTNACC	2 cut(s)	484, 1466
PspFI	CCCAGC	1 cut(s)	1283
PspGI	CCWGG	1 cut(s)	86
PspN4I	GGNNCC	5 cut(s)	211, 325, 370, 915, 1596
PspOMI	GGGCCC	1 cut(s)	913
PspPI	GGNCC	4 cut(s)	223, 913, 914, 1595
PsrI	GAACNNNNNNTAC	2 cut(s)	952, 984
PstNI	CAGNNNCTG	2 cut(s)	78, 710
PsuI	RGATCY	5 cut(s)	209, 323, 792, 898, 1384
RsaI	GTAC	4 cut(s)	266, 574, 855, 1512
RsaNI	GTAC	4 cut(s)	265, 573, 854, 1511
RseI	CAYNNNNRTG	2 cut(s)	872, 1275
SacI	GAGCTC	1 cut(s)	236
SaqAI	TTAA	6 cut(s)	363, 443, 1344, 1353, 1548, 1620
SatI	GCNGC	8 cut(s)	6, 48, 119, 146, 301, 372, 1206, 1506
Sau3AI	GATC	8 cut(s)	209, 323, 603, 792, 898, 1093, 1384, 1668
Sau96I	GGNCC	4 cut(s)	223, 913, 914, 1595
ScaI	AGTACT	1 cut(s)	266
ScrFI	CCNGG	2 cut(s)	88, 112
SduI	GDGCHC	4 cut(s)	236, 309, 917, 1075
SfaNI	GCATC	7 cut(s)	29, 287, 697, 793, 1115, 1237, 1490
SfcI	CTRYAG	3 cut(s)	268, 396, 1491
SinI	GGWCC	1 cut(s)	1595
SmiMI	CAYNNNNRTG	2 cut(s)	872, 1275
SmlI	CTYRAG	2 cut(s)	530, 1388
SmoI	CTYRAG	2 cut(s)	530, 1388
SpeI	ACTAGT	1 cut(s)	1588
SphI	GCATGC	1 cut(s)	1068
Sse9I	AATT	7 cut(s)	176, 523, 807, 919, 927, 1220, 1345
SsiI	CCGC	5 cut(s)	26, 118, 303, 372, 457
SspI	AATATT	1 cut(s)	1546
SspMI	CTAG	5 cut(s)	648, 740, 1172, 1332, 1589
SstI	GAGCTC	1 cut(s)	236
StyD4I	CCNGG	2 cut(s)	86, 110
StyI	CCWWGG	4 cut(s)	390, 699, 1115, 1225
TaaI	ACNGT	3 cut(s)	318, 1077, 1320
TaiI	ACGT	3 cut(s)	38, 68, 1620
TaqI	TCGA	3 cut(s)	249, 346, 1667
TaqII	GACCGA	1 cut(s)	49
TasI	AATT	7 cut(s)	176, 523, 807, 919, 927, 1220, 1345
TatI	WGTACW	2 cut(s)	264, 1510
TauI	GCSGC	2 cut(s)	121, 374
TfiI	GAWTC	4 cut(s)	503, 714, 1025, 1426
Tru1I	TTAA	6 cut(s)	363, 443, 1344, 1353, 1548, 1620
Tru9I	TTAA	6 cut(s)	363, 443, 1344, 1353, 1548, 1620
TscAI	CASTG	4 cut(s)	211, 321, 1035, 1080
TseFI	GTSAC	6 cut(s)	61, 312, 466, 484, 1077, 1466
TseI	GCWGC	6 cut(s)	5, 47, 145, 300, 1205, 1505
Tsp45I	GTSAC	6 cut(s)	61, 312, 466, 484, 1077, 1466
TspDTI	ATGAA	4 cut(s)	655, 803, 854, 1400
TspGWI	ACGGA	2 cut(s)	635, 639
TspRI	CASTG	4 cut(s)	211, 321, 1035, 1080
VpaK11BI	GGWCC	1 cut(s)	1595
VspI	ATTAAT	2 cut(s)	1353, 1548
XagI	CCTNNNNNAGG	1 cut(s)	112
XapI	RAATTY	2 cut(s)	176, 1220
XbaI	TCTAGA	2 cut(s)	647, 1171
XceI	RCATGY	5 cut(s)	58, 1068, 1190, 1245, 1364
XcmI	CCANNNNNNNNNTGG	1 cut(s)	892
XmnI	GAANNNNTTC	2 cut(s)	794, 845
XspI	CTAG	5 cut(s)	648, 740, 1172, 1332, 1589
ZrmI	AGTACT	1 cut(s)	266

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.