Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002195

Physical Location & Seq

Genomic Coordinates Forward (+)

17994550 .. 18000170

Gene Structure 5621 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1221800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1677 bp

ATGGCTGCCTCCTCTGTTGCATCTCCGCTCTGCACGTGGCTGGTGGCCGCTTGCATGTCGGTCACGTGCGACAGGGACTGCTCTGCCAGGCCATCTATGCTCAGCTCCTCCCGGAGGCGGCCGAAATGTGCCGGAAGAAGGAGGCTGCCGTCCAAATGTAGTAGCTTTAGTGGCGATTTCCAGAAGGGCCTGATTTCTGCGTTCAGTGGATCCAGCATTCAGGGCCTCATGAGCTCCTGCTTGGCTTTCGAGCCCTGCGATGAGTACTACAGCTCAAAGGGTTTATCTTCTTTGGGATGCAATGGGCTTTCATCACTGTTTGGATCCAATCGAAGGCAGAGGCAATTTAATGGAGCCTCCTACTCTGGGGAAACCATGGCTGTAGCTGTGCAACCTACTGAAGAATTAACAAAACAGAAGAAACCTTTAACAAGACAGAGGCGAGTGGTTGTGACAGGAATGAGTGTGGTGACCCCACTCGGTCATGATTCTGATGTCTTCTACAATAATTTGCTTGAGGGAGTTAGTGGCATTAGTGAGATAGAAGCTTTTGATTGTACCCAGTTTCCAACGAGGATAGGTGGTGAGATAAAGTCTTTCTCAACGGACGGATGGGTAACACCAAAGTTTTCTAGAAGAATGGATAAGTTCATGCTTTATTTGCTTACTGCTGGAAAGAAAGCCTTGGCAGATGCTGGAATCACAGAAGAAGTCCTGGAAACATTAGATAAAGCAAAATGTGGGGTTTTGATTGGCTCAGCAATGGGTGGCATGAAGGTCTTCAATGATGCAATTGAAGCTTTACGGGTTTCCTATAAGAAGATGAACCCTTTCTGTGTACCCTTCGCAACTACAAATATGGGTTCTGCCATGCTGGCAATGGATCTGGGCTGGATGGGCCCAAACTATTCAATCTCTACTGCTTGTGCTACAAGCAACTTCTGTATTTTGAATTCAGCAAATCATATCATTAGAGGCGAAGCAGACCTTATGCTCTGTGGTGGTTCAGATTCAGTGATGATACCTATTGGATTGGGAGGCTTTGTGGCATGCAGAGCACTGTCACAAAGAAACAATGATCCGACTAAAGCATCACGCCCTTGGGATAGTAATCGTGATGGCTTTGTTATGGGGGAAGGAGCTGGCGTTTTGCTTCTAGAAGAGTTAGAACATGCTAAGAAAAGAGGTGCAACTATTTATGCAGAATTCCTTGGTGGAAGCTTCACATGTGATGCTTACCACATGACTGAGCCACACCCTGATGGGGCCGGGGTTATTCTCTGCATAGAAAAGGCATTAGAGCAGTCTGGGGTGTCTAGGGAAGATGTTAATTACATTAATGCACATGCTACATCCACACCAGCCGGAGATCTCAAGGAGTATAATGCTCTCATTCATTGTTTTGGCCAGAACCCTGAGTTGAGAGTGAACTCTACAAAATCCATGATTGGTCACCTACTAGGAGCAGCTGGTGCTGTAGAAGCTGTTGCAGCAGTGCAGGCGATACGAACTGGGTGGATACATCCAAATATCAATCTGGAAAACCCAGATGAGGGAGTGGATACAAAACTACTAGTGGGTCCAAAGAAAGAGAGGCTGGACGTTAAGGTGGCATTGTCTAACTCATTTGGGTTCGGTGGCCACAACTCATCGGTCTTGTTTGCCCCATACCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004312 GO:0004315 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006636 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009266 GO:0009409 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009631 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016053 GO:0016740 GO:0016746 GO:0016747 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0032787 GO:0033559 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0071704 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00061 ko00780 ko01100 ko01212 map00061 map00780 map01100 map01212

Pfam Domains

Protein Families

Ketoacyl-synt_C ketoacyl-synt

Protein Analysis

559

Amino Acids

59.83

Weight (kDa)

7.45

Isoelectric Point (pI)

48.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ketoacyl-synt	PF00109	147 - 392	6.4e-59	Beta-ketoacyl synthase, N-terminal domain
Ketoacyl-synt_C	PF02801	400 - 514	1.3e-35	Beta-ketoacyl synthase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002195)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74960 AT1G74960 AT1G74960
fragaria_vesca	FvH4_1g09200 FvH4_1g09200 FvH4_1g09200 FvH4_1g09200 FvH4_6g35220 FvH4_6g35220
malus_domestica	MD02G1097200.v1.1 MD02G1097500.v1.1 MD02G1098500.v1.1 MD02G1101500.v1.1 MD09G1177500.v1.1 MD15G1221800.v1.1
prunus_persica	Prupe.3G036900_v2.0.a1 Prupe.3G036900_v2.0.a1 Prupe.7G193900_v2.0.a1 Prupe.7G193900_v2.0.a1 Prupe.7G193900_v2.0.a1
pyrus_communis	pycom02g07700 pycom15g19630
rosa_chinensis	RchiOBHm_Chr2g0095651 RchiOBHm_Chr2g0146561
rosa_laevigata	RLG00000016584 RLG00000020207
rosa_multiflora	Rmu_sc0001309.1_g000017 Rmu_sc0001986.1_g000028
rosa_roxburghii	Rroxscaffold_2G00100040 Rroxscaffold_2G00146150
rosa_rugosa	Rorug02G0052800 Rorug02G0394700 Rorug02G0394800 Rorug02G0394800
rosa_samantha	Rh2AG100800 Rh2AG451600 Rh2BG464400 Rh2CG104800 Rh2CG438500 Rh2DG102000 Rh2DG473400
rosa_wichuraiana	Rw2G007650 Rw2G036890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	28
AciI	CCGC	3 cut(s)	26, 48, 118
AclWI	GGATC	6 cut(s)	204, 217, 318, 331, 891, 1073
AcoI	YGGCCR	4 cut(s)	45, 119, 1405, 1639
AcsI	RAATTY	2 cut(s)	952, 1205
AcuI	CTGAAG	1 cut(s)	420
AcvI	CACGTG	2 cut(s)	36, 66
AfaI	GTAC	3 cut(s)	266, 559, 840
AfiI	CCNNNNNNNGG	8 cut(s)	114, 117, 138, 333, 366, 1264, 1552, 1553
AflIII	ACRYGT	1 cut(s)	1226
AgsI	TTSAA	4 cut(s)	784, 797, 912, 952
AhlI	ACTAGT	1 cut(s)	1573
AjnI	CCWGG	2 cut(s)	86, 714
Alw21I	GWGCWC	2 cut(s)	236, 1060
AlwI	GGATC	6 cut(s)	204, 217, 318, 331, 891, 1073
AlwNI	CAGNNNCTG	2 cut(s)	78, 695
AoxI	GGCC	9 cut(s)	45, 89, 119, 187, 223, 898, 1266, 1405, 1639
ApaI	GGGCCC	1 cut(s)	902
ApeKI	GCWGC	4 cut(s)	5, 145, 1466, 1490
ApoI	RAATTY	2 cut(s)	952, 1205
AseI	ATTAAT	1 cut(s)	1338
Asp700I	GAANNNNTTC	2 cut(s)	779, 830
AspS9I	GGNCC	6 cut(s)	187, 223, 898, 899, 1266, 1580
AsuC2I	CCSGG	2 cut(s)	112, 1270
AsuHPI	GGTGA	3 cut(s)	481, 596, 1445
AvaII	GGWCC	1 cut(s)	1580
BaeGI	GKGCMC	1 cut(s)	902
BalI	TGGCCA	2 cut(s)	1407, 1641
BamHI	GGATCC	2 cut(s)	209, 323
BanII	GRGCYC	3 cut(s)	236, 255, 902
BbrPI	CACGTG	2 cut(s)	36, 66
BbsI	GAAGAC	2 cut(s)	490, 772
Bbv12I	GWGCWC	2 cut(s)	236, 1060
BbvI	GCAGC	3 cut(s)	132, 1478, 1502
BccI	CCATC	5 cut(s)	100, 606, 889, 1112, 1256
BceAI	ACGGC	1 cut(s)	133
BciT130I	CCWGG	2 cut(s)	88, 716
BciVI	GTATCC	2 cut(s)	1512, 1555
BcnI	CCSGG	2 cut(s)	112, 1270
BcuI	ACTAGT	1 cut(s)	1573
BfaI	CTAG	5 cut(s)	633, 1157, 1317, 1460, 1574
BfmI	CTRYAG	3 cut(s)	268, 381, 1476
BfuI	GTATCC	2 cut(s)	1512, 1555
BglI	GCCNNNNNGGC	1 cut(s)	875
BglII	AGATCT	1 cut(s)	1369
BisI	GCNGC	6 cut(s)	6, 48, 119, 146, 1467, 1491
BlpI	GCTNAGC	2 cut(s)	101, 757
BlsI	GCNGC	6 cut(s)	7, 49, 120, 147, 1468, 1492
BmcAI	AGTACT	1 cut(s)	266
Bme1390I	CCNGG	4 cut(s)	88, 112, 716, 1270
Bme18I	GGWCC	1 cut(s)	1580
BmgT120I	GGNCC	6 cut(s)	187, 223, 898, 899, 1266, 1580
BmiI	GGNNCC	6 cut(s)	211, 325, 355, 900, 1267, 1581
BmrFI	CCNGG	4 cut(s)	88, 112, 716, 1270
BmrI	ACTGGG	2 cut(s)	556, 1521
BmsI	GCATC	6 cut(s)	29, 287, 682, 778, 1100, 1222
BmuI	ACTGGG	2 cut(s)	556, 1521
BpiI	GAAGAC	2 cut(s)	490, 772
Bpu1102I	GCTNAGC	2 cut(s)	101, 757
BpuEI	CTTGAG	2 cut(s)	536, 1358
BpuMI	CCSGG	2 cut(s)	112, 1270
BsaAI	YACGTR	2 cut(s)	36, 66
BsaBI	GATNNNNATC	1 cut(s)	1110
BsaJI	CCNNGG	5 cut(s)	375, 684, 1100, 1210, 1269
BsaXI	ACNNNNNCTCC	4 cut(s)	1029, 1059, 1131, 1161
Bsc4I	CCNNNNNNNGG	8 cut(s)	114, 117, 138, 333, 366, 1264, 1552, 1553
Bse1I	ACTGG	3 cut(s)	562, 1516, 1672
Bse3DI	GCAATG	3 cut(s)	307, 768, 885
Bse8I	GATNNNNATC	1 cut(s)	1110
BseBI	CCWGG	2 cut(s)	88, 716
BseDI	CCNNGG	5 cut(s)	375, 684, 1100, 1210, 1269
BseGI	GGATG	5 cut(s)	302, 617, 900, 1352, 1522
BseJI	GATNNNNATC	1 cut(s)	1110
BseLI	CCNNNNNNNGG	8 cut(s)	114, 117, 138, 333, 366, 1264, 1552, 1553
BseMI	GCAATG	3 cut(s)	307, 768, 885
BseMII	CTCAG	4 cut(s)	115, 771, 1239, 1407
BseNI	ACTGG	3 cut(s)	562, 1516, 1672
BseRI	GAGGAG	1 cut(s)	97
BseSI	GKGCMC	1 cut(s)	902
BseX3I	CGGCCG	1 cut(s)	119
BseXI	GCAGC	3 cut(s)	132, 1478, 1502
BsgI	GTGCAG	2 cut(s)	16, 1517
Bsh1285I	CGRYCG	1 cut(s)	122
BshFI	GGCC	9 cut(s)	47, 91, 121, 189, 225, 900, 1268, 1407, 1641
BsiEI	CGRYCG	1 cut(s)	122
BsiHKAI	GWGCWC	2 cut(s)	236, 1060
BsiSI	CCGG	4 cut(s)	112, 132, 1269, 1365
BslFI	GGGAC	1 cut(s)	89
BslI	CCNNNNNNNGG	8 cut(s)	114, 117, 138, 333, 366, 1264, 1552, 1553
BsmFI	GGGAC	1 cut(s)	89
BsmI	GAATGC	1 cut(s)	216
BsnI	GGCC	9 cut(s)	47, 91, 121, 189, 225, 900, 1268, 1407, 1641
Bsp120I	GGGCCC	1 cut(s)	898
Bsp1286I	GDGCHC	4 cut(s)	236, 255, 902, 1060
Bsp143I	GATC	5 cut(s)	209, 323, 883, 1078, 1369
Bsp1720I	GCTNAGC	2 cut(s)	101, 757
Bsp19I	CCATGG	1 cut(s)	375
BspACI	CCGC	3 cut(s)	26, 48, 118
BspANI	GGCC	9 cut(s)	47, 91, 121, 189, 225, 900, 1268, 1407, 1641
BspCNI	CTCAG	4 cut(s)	114, 770, 1240, 1408
BspHI	TCATGA	2 cut(s)	228, 484
BspLI	GGNNCC	6 cut(s)	211, 325, 355, 900, 1267, 1581
BspPI	GGATC	6 cut(s)	204, 217, 318, 331, 891, 1073
BsrBI	CCGCTC	1 cut(s)	28
BsrDI	GCAATG	3 cut(s)	307, 768, 885
BsrI	ACTGG	3 cut(s)	562, 1516, 1672
BssECI	CCNNGG	5 cut(s)	375, 684, 1100, 1210, 1269
BssMI	GATC	5 cut(s)	209, 323, 883, 1078, 1369
BssT1I	CCWWGG	4 cut(s)	375, 684, 1100, 1210
Bst2UI	CCWGG	2 cut(s)	88, 716
Bst4CI	ACNGT	2 cut(s)	318, 1062
Bst6I	CTCTTC	1 cut(s)	1155
BstAPI	GCANNNNNTGC	1 cut(s)	1472
BstBAI	YACGTR	2 cut(s)	36, 66
BstC8I	GCNNGC	5 cut(s)	52, 876, 1051, 1144, 1500
BstDEI	CTNAG	5 cut(s)	101, 757, 1176, 1248, 1416
BstDSI	CCRYGG	1 cut(s)	375
BstEII	GGTNACC	2 cut(s)	469, 1451
BstENI	CCTNNNNNAGG	1 cut(s)	112
BstF5I	GGATG	5 cut(s)	302, 617, 900, 1352, 1522
BstKTI	GATC	5 cut(s)	212, 326, 886, 1081, 1372
BstMBI	GATC	5 cut(s)	209, 323, 883, 1078, 1369
BstMCI	CGRYCG	1 cut(s)	122
BstNI	CCWGG	2 cut(s)	88, 716
BstNSI	RCATGY	5 cut(s)	58, 1053, 1175, 1230, 1349
BstPI	GGTNACC	2 cut(s)	469, 1451
BstSCI	CCNGG	4 cut(s)	86, 110, 714, 1268
BstSFI	CTRYAG	3 cut(s)	268, 381, 1476
BstSLI	GKGCMC	1 cut(s)	902
BstV1I	GCAGC	3 cut(s)	132, 1478, 1502
BstV2I	GAAGAC	2 cut(s)	490, 772
BstX2I	RGATCY	4 cut(s)	209, 323, 883, 1369
BstYI	RGATCY	4 cut(s)	209, 323, 883, 1369
BstZI	CGGCCG	1 cut(s)	119
BsuI	GTATCC	2 cut(s)	1512, 1555
BsuRI	GGCC	9 cut(s)	47, 91, 121, 189, 225, 900, 1268, 1407, 1641
BtgI	CCRYGG	1 cut(s)	375
BtgZI	GCGATG	1 cut(s)	273
BtsCI	GGATG	5 cut(s)	302, 617, 900, 1352, 1522
BtsI	GCAGTG	1 cut(s)	1500
BtsIMutI	CAGTG	5 cut(s)	211, 314, 1020, 1058, 1500
Cac8I	GCNNGC	5 cut(s)	52, 876, 1051, 1144, 1500
CaiI	CAGNNNCTG	2 cut(s)	78, 695
CciI	TCATGA	2 cut(s)	228, 484
Cfr13I	GGNCC	6 cut(s)	187, 223, 898, 899, 1266, 1580
Csp6I	GTAC	3 cut(s)	265, 558, 839
CviQI	GTAC	3 cut(s)	265, 558, 839
DdeI	CTNAG	5 cut(s)	101, 757, 1176, 1248, 1416
DpnI	GATC	5 cut(s)	211, 325, 885, 1080, 1371
DpnII	GATC	5 cut(s)	209, 323, 883, 1078, 1369
EaeI	YGGCCR	4 cut(s)	45, 119, 1405, 1639
EagI	CGGCCG	1 cut(s)	119
Eam1104I	CTCTTC	1 cut(s)	1155
EarI	CTCTTC	1 cut(s)	1155
Ecl136II	GAGCTC	1 cut(s)	234
EclXI	CGGCCG	1 cut(s)	119
Eco130I	CCWWGG	4 cut(s)	375, 684, 1100, 1210
Eco24I	GRGCYC	3 cut(s)	236, 255, 902
Eco47I	GGWCC	1 cut(s)	1580
Eco52I	CGGCCG	1 cut(s)	119
Eco53kI	GAGCTC	1 cut(s)	234
Eco57I	CTGAAG	1 cut(s)	420
Eco72I	CACGTG	2 cut(s)	36, 66
Eco91I	GGTNACC	2 cut(s)	469, 1451
EcoICRI	GAGCTC	1 cut(s)	234
EcoNI	CCTNNNNNAGG	1 cut(s)	112
EcoO109I	RGGNCCY	2 cut(s)	187, 223
EcoO65I	GGTNACC	2 cut(s)	469, 1451
EcoRI	GAATTC	2 cut(s)	952, 1205
EcoRII	CCWGG	2 cut(s)	86, 714
EcoT14I	CCWWGG	4 cut(s)	375, 684, 1100, 1210
EcoT38I	GRGCYC	3 cut(s)	236, 255, 902
ErhI	CCWWGG	4 cut(s)	375, 684, 1100, 1210
FalI	AAGNNNNNCTT	4 cut(s)	668, 700, 972, 1004
FaqI	GGGAC	1 cut(s)	89
Fnu4HI	GCNGC	6 cut(s)	6, 48, 119, 146, 1467, 1491
FokI	GGATG	5 cut(s)	309, 624, 907, 1339, 1509
FriOI	GRGCYC	3 cut(s)	236, 255, 902
Fsp4HI	GCNGC	6 cut(s)	6, 48, 119, 146, 1467, 1491
FspBI	CTAG	5 cut(s)	633, 1157, 1317, 1460, 1574
GluI	GCNGC	6 cut(s)	6, 48, 119, 146, 1467, 1491
HaeIII	GGCC	9 cut(s)	47, 91, 121, 189, 225, 900, 1268, 1407, 1641
HapII	CCGG	4 cut(s)	112, 132, 1269, 1365
HindIII	AAGCTT	3 cut(s)	546, 798, 1219
HinfI	GANTC	3 cut(s)	488, 699, 1010
HpaII	CCGG	4 cut(s)	112, 132, 1269, 1365
HphI	GGTGA	3 cut(s)	481, 596, 1445
Hpy166II	GTNNAC	2 cut(s)	839, 1429
Hpy188I	TCNGA	3 cut(s)	493, 1009, 1083
Hpy188III	TCNNGA	7 cut(s)	181, 229, 485, 633, 1115, 1157, 1538
Hpy8I	GTNNAC	2 cut(s)	839, 1429
HpyAV	CCTTC	6 cut(s)	132, 178, 327, 769, 853, 1130
HpyCH4III	ACNGT	2 cut(s)	318, 1062
HpyCH4IV	ACGT	3 cut(s)	35, 65, 1602
HpyF3I	CTNAG	5 cut(s)	101, 757, 1176, 1248, 1416
HpySE526I	ACGT	3 cut(s)	35, 65, 1602
Kzo9I	GATC	5 cut(s)	209, 323, 883, 1078, 1369
LmnI	GCTCC	5 cut(s)	110, 239, 353, 1139, 1463
Lsp1109I	GCAGC	3 cut(s)	132, 1478, 1502
LweI	GCATC	6 cut(s)	29, 287, 682, 778, 1100, 1222
MaeI	CTAG	5 cut(s)	633, 1157, 1317, 1460, 1574
MaeII	ACGT	3 cut(s)	35, 65, 1602
MaeIII	GTNAC	6 cut(s)	61, 451, 469, 616, 1062, 1451
MalI	GATC	5 cut(s)	211, 325, 885, 1080, 1371
MbiI	CCGCTC	1 cut(s)	28
MboI	GATC	5 cut(s)	209, 323, 883, 1078, 1369
MfeI	CAATTG	1 cut(s)	792
MflI	RGATCY	4 cut(s)	209, 323, 883, 1369
MhlI	GDGCHC	4 cut(s)	236, 255, 902, 1060
MlsI	TGGCCA	2 cut(s)	1407, 1641
MluCI	AATT	7 cut(s)	344, 404, 508, 792, 952, 1205, 1330
MluNI	TGGCCA	2 cut(s)	1407, 1641
MmeI	TCCRAC	2 cut(s)	593, 1106
Mox20I	TGGCCA	2 cut(s)	1407, 1641
MroXI	GAANNNNTTC	2 cut(s)	779, 830
MscI	TGGCCA	2 cut(s)	1407, 1641
MseI	TTAA	6 cut(s)	348, 407, 428, 1329, 1338, 1605
MslI	CAYNNNNRTG	1 cut(s)	1260
Msp20I	TGGCCA	2 cut(s)	1407, 1641
MspA1I	CMGCKG	1 cut(s)	1469
MspI	CCGG	4 cut(s)	112, 132, 1269, 1365
MspR9I	CCNGG	4 cut(s)	88, 112, 716, 1270
MunI	CAATTG	1 cut(s)	792
Mva1269I	GAATGC	1 cut(s)	216
MvaI	CCWGG	2 cut(s)	88, 716
NciI	CCSGG	2 cut(s)	112, 1270
NcoI	CCATGG	1 cut(s)	375
NdeII	GATC	5 cut(s)	209, 323, 883, 1078, 1369
NlaIV	GGNNCC	6 cut(s)	211, 325, 355, 900, 1267, 1581
NmuCI	GTSAC	5 cut(s)	61, 451, 469, 1062, 1451
NspI	RCATGY	5 cut(s)	58, 1053, 1175, 1230, 1349
PaeI	GCATGC	1 cut(s)	1053
PagI	TCATGA	2 cut(s)	228, 484
PciI	ACATGT	1 cut(s)	1226
PcsI	WCGNNNNNNNCGW	1 cut(s)	255
PctI	GAATGC	1 cut(s)	216
PdmI	GAANNNNTTC	2 cut(s)	779, 830
PfeI	GAWTC	3 cut(s)	488, 699, 1010
PfoI	TCCNGGA	2 cut(s)	110, 714
PkrI	GCNGC	6 cut(s)	7, 49, 120, 147, 1468, 1492
PmaCI	CACGTG	2 cut(s)	36, 66
PmlI	CACGTG	2 cut(s)	36, 66
Ppu21I	YACGTR	2 cut(s)	36, 66
PscI	ACATGT	1 cut(s)	1226
PshBI	ATTAAT	1 cut(s)	1338
Psp124BI	GAGCTC	1 cut(s)	236
Psp6I	CCWGG	2 cut(s)	86, 714
PspCI	CACGTG	2 cut(s)	36, 66
PspEI	GGTNACC	2 cut(s)	469, 1451
PspGI	CCWGG	2 cut(s)	86, 714
PspN4I	GGNNCC	6 cut(s)	211, 325, 355, 900, 1267, 1581
PspOMI	GGGCCC	1 cut(s)	898
PspPI	GGNCC	6 cut(s)	187, 223, 898, 899, 1266, 1580
PstNI	CAGNNNCTG	2 cut(s)	78, 695
PsuI	RGATCY	4 cut(s)	209, 323, 883, 1369
PvuII	CAGCTG	1 cut(s)	1469
RsaI	GTAC	3 cut(s)	266, 559, 840
RsaNI	GTAC	3 cut(s)	265, 558, 839
RseI	CAYNNNNRTG	1 cut(s)	1260
SacI	GAGCTC	1 cut(s)	236
SaqAI	TTAA	6 cut(s)	348, 407, 428, 1329, 1338, 1605
SatI	GCNGC	6 cut(s)	6, 48, 119, 146, 1467, 1491
Sau3AI	GATC	5 cut(s)	209, 323, 883, 1078, 1369
Sau96I	GGNCC	6 cut(s)	187, 223, 898, 899, 1266, 1580
ScaI	AGTACT	1 cut(s)	266
ScrFI	CCNGG	4 cut(s)	88, 112, 716, 1270
SduI	GDGCHC	4 cut(s)	236, 255, 902, 1060
SfaNI	GCATC	6 cut(s)	29, 287, 682, 778, 1100, 1222
SfcI	CTRYAG	3 cut(s)	268, 381, 1476
SinI	GGWCC	1 cut(s)	1580
SmiMI	CAYNNNNRTG	1 cut(s)	1260
SmlI	CTYRAG	2 cut(s)	515, 1373
SmoI	CTYRAG	2 cut(s)	515, 1373
SpeI	ACTAGT	1 cut(s)	1573
SphI	GCATGC	1 cut(s)	1053
Sse9I	AATT	7 cut(s)	344, 404, 508, 792, 952, 1205, 1330
SsiI	CCGC	3 cut(s)	26, 48, 118
SspMI	CTAG	5 cut(s)	633, 1157, 1317, 1460, 1574
SstI	GAGCTC	1 cut(s)	236
StyD4I	CCNGG	4 cut(s)	86, 110, 714, 1268
StyI	CCWWGG	4 cut(s)	375, 684, 1100, 1210
TaaI	ACNGT	2 cut(s)	318, 1062
TaiI	ACGT	3 cut(s)	38, 68, 1605
TaqI	TCGA	2 cut(s)	249, 331
TaqII	GACCGA	3 cut(s)	49, 470, 1642
TasI	AATT	7 cut(s)	344, 404, 508, 792, 952, 1205, 1330
TatI	WGTACW	1 cut(s)	264
TauI	GCSGC	2 cut(s)	50, 121
TfiI	GAWTC	3 cut(s)	488, 699, 1010
Tru1I	TTAA	6 cut(s)	348, 407, 428, 1329, 1338, 1605
Tru9I	TTAA	6 cut(s)	348, 407, 428, 1329, 1338, 1605
TscAI	CASTG	5 cut(s)	211, 321, 1020, 1065, 1500
TseFI	GTSAC	5 cut(s)	61, 451, 469, 1062, 1451
TseI	GCWGC	4 cut(s)	5, 145, 1466, 1490
Tsp45I	GTSAC	5 cut(s)	61, 451, 469, 1062, 1451
TspDTI	ATGAA	5 cut(s)	300, 640, 788, 839, 1385
TspGWI	ACGGA	2 cut(s)	620, 624
TspRI	CASTG	5 cut(s)	211, 321, 1020, 1065, 1500
VpaK11BI	GGWCC	1 cut(s)	1580
VspI	ATTAAT	1 cut(s)	1338
XagI	CCTNNNNNAGG	1 cut(s)	112
XapI	RAATTY	2 cut(s)	952, 1205
XbaI	TCTAGA	2 cut(s)	632, 1156
XceI	RCATGY	5 cut(s)	58, 1053, 1175, 1230, 1349
XcmI	CCANNNNNNNNNTGG	1 cut(s)	877
XmnI	GAANNNNTTC	2 cut(s)	779, 830
XspI	CTAG	5 cut(s)	633, 1157, 1317, 1460, 1574
ZrmI	AGTACT	1 cut(s)	266

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.