Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002195

Physical Location & Seq

Genomic Coordinates Forward (+)

7933586 .. 7939239

Gene Structure 5654 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016584

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1626 bp

ATGGCTGCCGCCACTTCGGCCGCGTCCCCTCTCTGCACGTGGCTCGTCGCCGCTTGCATGTCGGTCACGTGCGACGGGGACTACTCCGCCAGGCCGTCACGTGCCAAGTTGGGCGGCGGCCGGAGACGGAGGCTGGCGAAATGTAGTGGCAGCTACTGCTTAAGTAGTAGGGGCGGATCCAGCATTCAGGGCCTGATGAGTTCTTGTTTGGCGTTTGAGCCTTGCAATGAGTACTATAGCTCTCGGGGTTTATCTTCTTTGGGCTCAAATGGGCTGTCCTCGCTGTTTGGATCCAAAACTGGGTCTTTGAATCGAAGCAGGAGGCGCTTTAATGCCCATTCTGGGGAAGCCATGGCTATTGCTGTGCAACCTACTGAAGAATTATCAAAACAGAAGAAACCTATAACAAGACAGAGGCGAGTGGTTGTGACAGGAATGAGTGTGGTGACCCCACTTGGTCATGATGCTGATGTTTTCTACAATAATCTGCTTGAGGGTGTTAGTGGCATTAGTGAGATAGAAGCTTTTGATTGTTCCCCATTTCCTACGAAAATTGGTGGGGAGATCAAGTCCTTTTCAACTGATGGATGGGTAGCACCAAAGTTTTCCAGAAGAATGGATAAATTCATGCTTTACTTGCTTACTGCTGGAAAGAAAGCCTTGGCAGATGGTGGAATTACAGAAGAAGTCATGGAGAAAATTGATAAAACTAAATGTGGGGTGTTGATCGGTTCTGCAATGGGTGGAATGAAGATCTTCAATGATGCAATTGAAGCTTTACGGGTTTCATATAAGAAGATGAATCCTTTCTGTGTGCCTTTCGCAACTACAAATATGGGTTCTGCCATGCTCGCAATGGATCTGGGTTGGATGGGCCCAAATTATTCAATCTCTACTGCTTGTGCTACAAGCAACTTCTGCATTTTGAATTCAGCAAATCATATCATTAGAGGTGAAGCTGACATTATGCTTTCTGGTGGTTCAGATTCAGTAATGATACCTATTGGCTTGGGAGGCTTTGTGGCATGCAGAGCACTTTCACAAAGGAACAGTGATCCAACTAAAGCATCACGACCTTGGGATACTAATCGTGATGGATTTGTTATGGGGGAAGGAGCTGGAGTGTTGCTTCTAGAAGAGTTAGAACATGCTAAGAAAAGAGGTGCAACCATCTATGCAGAGTTTCTAGGTGGAAGCTTCACATGTGATGCTTACCACATGACTGAGCCACGCCCTGATGGGGTTGGAGTTATTCAATGCATAGAGAAGGCATTAACTCAGTCTGGCGTATCTAAGGAGGATGTCAATTACATTAATGCACATGCAACATCCACACCAGCTGGAGACCTTAAAGAGTATAATGCTCTGATGCGTTGTTTTGGCCAGAATCCTGAGTTGAGAGTCAACTCTACAAAATCCATGATTGGTCACCTGCTAGGAGCAGCTGGCGCAGTGGAGGCCGTTGCAGCAGTACAGGCAATACGAACTGGATGGGTTCATCCAAATATCAATCTGGACGACCCAGATGAGGGTGTGGATGTGAAAGTGCTATTTTGTTTGCTCCTTACAAGTAGTAACACAGATCTAGTGACTACTCCAACTTTTACTTCGAACTCACTGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004312 GO:0004315 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006636 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009266 GO:0009409 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009631 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016053 GO:0016740 GO:0016746 GO:0016747 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0032787 GO:0033559 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0071704 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00061 ko00780 ko01100 ko01212 map00061 map00780 map01100 map01212

Pfam Domains

Protein Families

Ketoacyl-synt_C ketoacyl-synt

Protein Analysis

542

Amino Acids

57.7

Weight (kDa)

8.23

Isoelectric Point (pI)

47.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ketoacyl-synt	PF00109	139 - 384	6.7e-57	Beta-ketoacyl synthase, N-terminal domain
Ketoacyl-synt_C	PF02801	392 - 505	3.3e-36	Beta-ketoacyl synthase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002195)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74960 AT1G74960 AT1G74960
fragaria_vesca	FvH4_1g09200 FvH4_1g09200 FvH4_1g09200 FvH4_1g09200 FvH4_6g35220 FvH4_6g35220
malus_domestica	MD02G1097200.v1.1 MD02G1097500.v1.1 MD02G1098500.v1.1 MD02G1101500.v1.1 MD09G1177500.v1.1 MD15G1221800.v1.1
prunus_persica	Prupe.3G036900_v2.0.a1 Prupe.3G036900_v2.0.a1 Prupe.7G193900_v2.0.a1 Prupe.7G193900_v2.0.a1 Prupe.7G193900_v2.0.a1
pyrus_communis	pycom02g07700 pycom15g19630
rosa_chinensis	RchiOBHm_Chr2g0095651 RchiOBHm_Chr2g0146561
rosa_laevigata	RLG00000016584 RLG00000020207
rosa_multiflora	Rmu_sc0001309.1_g000017 Rmu_sc0001986.1_g000028
rosa_roxburghii	Rroxscaffold_2G00100040 Rroxscaffold_2G00146150
rosa_rugosa	Rorug02G0052800 Rorug02G0394700 Rorug02G0394800 Rorug02G0394800
rosa_samantha	Rh2AG100800 Rh2AG451600 Rh2BG464400 Rh2CG104800 Rh2CG438500 Rh2DG102000 Rh2DG473400
rosa_wichuraiana	Rw2G007650 Rw2G036890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1440
Acc36I	ACCTGC	1 cut(s)	1440
AccII	CGCG	1 cut(s)	23
AciI	CCGC	7 cut(s)	9, 21, 51, 87, 114, 117, 174
AclWI	GGATC	6 cut(s)	171, 184, 285, 298, 867, 1049
AcoI	YGGCCR	3 cut(s)	18, 118, 1381
AcsI	RAATTY	2 cut(s)	623, 928
AcuI	CTGAAG	1 cut(s)	396
AcvI	CACGTG	3 cut(s)	39, 69, 101
AfaI	GTAC	2 cut(s)	233, 1473
AfiI	CCNNNNNNNGG	6 cut(s)	300, 342, 343, 1240, 1528, 1529
AflII	CTTAAG	1 cut(s)	160
AflIII	ACRYGT	1 cut(s)	1202
AgsI	TTSAA	7 cut(s)	310, 579, 760, 773, 888, 928, 1256
AjnI	CCWGG	1 cut(s)	89
AjuI	GAANNNNNNNTTGG	2 cut(s)	1591, 1623
AleI	CACNNNNGTG	1 cut(s)	1621
AluBI	AGCT	9 cut(s)	153, 240, 524, 776, 959, 1118, 1197, 1340, 1445
AluI	AGCT	9 cut(s)	153, 240, 524, 776, 959, 1118, 1197, 1340, 1445
Alw21I	GWGCWC	1 cut(s)	1036
Alw26I	GTCTC	2 cut(s)	118, 1338
AlwI	GGATC	6 cut(s)	171, 184, 285, 298, 867, 1049
AlwNI	CAGNNNCTG	2 cut(s)	156, 193
Ama87I	CYCGRG	1 cut(s)	243
AoxI	GGCC	7 cut(s)	18, 92, 118, 190, 874, 1381, 1458
ApaI	GGGCCC	1 cut(s)	878
ApeKI	GCWGC	4 cut(s)	5, 150, 1442, 1466
ApoI	RAATTY	2 cut(s)	623, 928
ArsI	GACNNNNNNTTYG	2 cut(s)	260, 292
AseI	ATTAAT	1 cut(s)	1314
Asp700I	GAANNNNTTC	2 cut(s)	755, 806
AspLEI	GCGC	2 cut(s)	327, 1451
AspS9I	GGNCC	3 cut(s)	190, 874, 875
AsuHPI	GGTGA	3 cut(s)	457, 965, 1421
AsuII	TTCGAA	1 cut(s)	1610
AvaI	CYCGRG	1 cut(s)	243
BaeGI	GKGCMC	1 cut(s)	878
BalI	TGGCCA	1 cut(s)	1383
BamHI	GGATCC	2 cut(s)	176, 290
BanII	GRGCYC	2 cut(s)	266, 878
BbrPI	CACGTG	3 cut(s)	39, 69, 101
Bbv12I	GWGCWC	1 cut(s)	1036
BbvI	GCAGC	3 cut(s)	162, 1454, 1478
BccI	CCATC	8 cut(s)	578, 582, 662, 865, 1088, 1178, 1232, 1485
BceAI	ACGGC	2 cut(s)	79, 1445
BcgI	CGANNNNNNTGC	2 cut(s)	25, 59
BciT130I	CCWGG	1 cut(s)	91
BciVI	GTATCC	1 cut(s)	1075
BcoDI	GTCTC	2 cut(s)	118, 1338
BfaI	CTAG	4 cut(s)	1133, 1187, 1436, 1586
BfmI	CTRYAG	1 cut(s)	235
BfoI	RGCGCY	1 cut(s)	328
BfrI	CTTAAG	1 cut(s)	160
BfuAI	ACCTGC	1 cut(s)	1440
BfuI	GTATCC	1 cut(s)	1075
BglI	GCCNNNNNGGC	1 cut(s)	17
BglII	AGATCT	2 cut(s)	753, 1582
BisI	GCNGC	9 cut(s)	6, 9, 21, 51, 115, 118, 151, 1443, 1467
BlsI	GCNGC	9 cut(s)	7, 10, 22, 52, 116, 119, 152, 1444, 1468
BmcAI	AGTACT	1 cut(s)	233
Bme1390I	CCNGG	1 cut(s)	91
BmeT110I	CYCGRG	1 cut(s)	243
BmgT120I	GGNCC	3 cut(s)	190, 874, 875
BmiI	GGNNCC	3 cut(s)	178, 292, 876
BmrFI	CCNGG	1 cut(s)	91
BmrI	ACTGGG	1 cut(s)	309
BmsI	GCATC	5 cut(s)	454, 754, 1076, 1198, 1359
BmuI	ACTGGG	1 cut(s)	309
BpmI	CTGGAG	2 cut(s)	1140, 1362
Bpu14I	TTCGAA	1 cut(s)	1610
BpuEI	CTTGAG	1 cut(s)	512
BsaAI	YACGTR	3 cut(s)	39, 69, 101
BsaBI	GATNNNNATC	1 cut(s)	1086
BsaI	GGTCTC	1 cut(s)	1338
BsaJI	CCNNGG	3 cut(s)	351, 660, 1076
BsaXI	ACNNNNNCTCC	6 cut(s)	554, 584, 1005, 1035, 1107, 1137
Bsc4I	CCNNNNNNNGG	6 cut(s)	300, 342, 343, 1240, 1528, 1529
Bse1I	ACTGG	2 cut(s)	304, 1492
Bse3DI	GCAATG	3 cut(s)	232, 744, 861
Bse8I	GATNNNNATC	1 cut(s)	1086
BseBI	CCWGG	1 cut(s)	91
BseDI	CCNNGG	3 cut(s)	351, 660, 1076
BseGI	GGATG	7 cut(s)	593, 876, 1306, 1328, 1496, 1498, 1543
BseJI	GATNNNNATC	1 cut(s)	1086
BseLI	CCNNNNNNNGG	6 cut(s)	300, 342, 343, 1240, 1528, 1529
BseMI	GCAATG	3 cut(s)	232, 744, 861
BseMII	CTCAG	3 cut(s)	1215, 1292, 1383
BseNI	ACTGG	2 cut(s)	304, 1492
BseSI	GKGCMC	1 cut(s)	878
BseX3I	CGGCCG	2 cut(s)	18, 118
BseXI	GCAGC	3 cut(s)	162, 1454, 1478
BsgI	GTGCAG	1 cut(s)	19
Bsh1236I	CGCG	1 cut(s)	23
Bsh1285I	CGRYCG	2 cut(s)	21, 121
BshFI	GGCC	7 cut(s)	20, 94, 120, 192, 876, 1383, 1460
BsiEI	CGRYCG	2 cut(s)	21, 121
BsiHKAI	GWGCWC	1 cut(s)	1036
BsiHKCI	CYCGRG	1 cut(s)	243
BsiSI	CCGG	1 cut(s)	121
BslFI	GGGAC	2 cut(s)	10, 92
BslI	CCNNNNNNNGG	6 cut(s)	300, 342, 343, 1240, 1528, 1529
BsmAI	GTCTC	2 cut(s)	118, 1338
BsmBI	CGTCTC	1 cut(s)	118
BsmFI	GGGAC	2 cut(s)	10, 92
BsmI	GAATGC	1 cut(s)	183
BsnI	GGCC	7 cut(s)	20, 94, 120, 192, 876, 1383, 1460
Bso31I	GGTCTC	1 cut(s)	1338
BsoBI	CYCGRG	1 cut(s)	243
Bsp119I	TTCGAA	1 cut(s)	1610
Bsp120I	GGGCCC	1 cut(s)	874
Bsp1286I	GDGCHC	3 cut(s)	266, 878, 1036
Bsp143I	GATC	8 cut(s)	176, 290, 564, 726, 753, 859, 1054, 1582
Bsp19I	CCATGG	1 cut(s)	351
BspACI	CCGC	7 cut(s)	9, 21, 51, 87, 114, 117, 174
BspANI	GGCC	7 cut(s)	20, 94, 120, 192, 876, 1383, 1460
BspCNI	CTCAG	3 cut(s)	1216, 1291, 1384
BspFNI	CGCG	1 cut(s)	23
BspHI	TCATGA	1 cut(s)	460
BspLI	GGNNCC	3 cut(s)	178, 292, 876
BspMI	ACCTGC	1 cut(s)	1440
BspPI	GGATC	6 cut(s)	171, 184, 285, 298, 867, 1049
BspT104I	TTCGAA	1 cut(s)	1610
BspTI	CTTAAG	1 cut(s)	160
BspTNI	GGTCTC	1 cut(s)	1338
BsrDI	GCAATG	3 cut(s)	232, 744, 861
BsrI	ACTGG	2 cut(s)	304, 1492
BssECI	CCNNGG	3 cut(s)	351, 660, 1076
BssMI	GATC	8 cut(s)	176, 290, 564, 726, 753, 859, 1054, 1582
BssT1I	CCWWGG	3 cut(s)	351, 660, 1076
Bst2UI	CCWGG	1 cut(s)	91
Bst4CI	ACNGT	1 cut(s)	1052
Bst6I	CTCTTC	1 cut(s)	1131
BstAFI	CTTAAG	1 cut(s)	160
BstAPI	GCANNNNNTGC	2 cut(s)	156, 918
BstBAI	YACGTR	3 cut(s)	39, 69, 101
BstBI	TTCGAA	1 cut(s)	1610
BstC8I	GCNNGC	5 cut(s)	55, 135, 852, 1027, 1447
BstDEI	CTNAG	5 cut(s)	1152, 1224, 1278, 1293, 1392
BstDSI	CCRYGG	1 cut(s)	351
BstEII	GGTNACC	2 cut(s)	445, 1427
BstF5I	GGATG	7 cut(s)	593, 876, 1306, 1328, 1496, 1498, 1543
BstFNI	CGCG	1 cut(s)	23
BstH2I	RGCGCY	1 cut(s)	328
BstHHI	GCGC	2 cut(s)	327, 1451
BstKTI	GATC	8 cut(s)	179, 293, 567, 729, 756, 862, 1057, 1585
BstMAI	GTCTC	2 cut(s)	118, 1338
BstMBI	GATC	8 cut(s)	176, 290, 564, 726, 753, 859, 1054, 1582
BstMCI	CGRYCG	2 cut(s)	21, 121
BstNI	CCWGG	1 cut(s)	91
BstNSI	RCATGY	5 cut(s)	61, 1029, 1151, 1206, 1325
BstPI	GGTNACC	2 cut(s)	445, 1427
BstSCI	CCNGG	1 cut(s)	89
BstSFI	CTRYAG	1 cut(s)	235
BstSLI	GKGCMC	1 cut(s)	878
BstUI	CGCG	1 cut(s)	23
BstV1I	GCAGC	3 cut(s)	162, 1454, 1478
BstX2I	RGATCY	5 cut(s)	176, 290, 753, 859, 1582
BstXI	CCANNNNNNTGG	1 cut(s)	616
BstYI	RGATCY	5 cut(s)	176, 290, 753, 859, 1582
BstZI	CGGCCG	2 cut(s)	18, 118
BsuI	GTATCC	1 cut(s)	1075
BsuRI	GGCC	7 cut(s)	20, 94, 120, 192, 876, 1383, 1460
BtgI	CCRYGG	1 cut(s)	351
BtsCI	GGATG	7 cut(s)	593, 876, 1306, 1328, 1496, 1498, 1543
BtsI	GCAGTG	1 cut(s)	1458
BtsIMutI	CAGTG	3 cut(s)	1057, 1458, 1616
BveI	ACCTGC	1 cut(s)	1440
Cac8I	GCNNGC	5 cut(s)	55, 135, 852, 1027, 1447
CaiI	CAGNNNCTG	2 cut(s)	156, 193
CciI	TCATGA	1 cut(s)	460
CfoI	GCGC	2 cut(s)	327, 1451
Cfr13I	GGNCC	3 cut(s)	190, 874, 875
CseI	GACGC	1 cut(s)	12
Csp6I	GTAC	2 cut(s)	232, 1472
CviQI	GTAC	2 cut(s)	232, 1472
DdeI	CTNAG	5 cut(s)	1152, 1224, 1278, 1293, 1392
DpnI	GATC	8 cut(s)	178, 292, 566, 728, 755, 861, 1056, 1584
DpnII	GATC	8 cut(s)	176, 290, 564, 726, 753, 859, 1054, 1582
EaeI	YGGCCR	3 cut(s)	18, 118, 1381
EagI	CGGCCG	2 cut(s)	18, 118
Eam1104I	CTCTTC	1 cut(s)	1131
EarI	CTCTTC	1 cut(s)	1131
EciI	GGCGGA	2 cut(s)	76, 189
EclXI	CGGCCG	2 cut(s)	18, 118
Eco130I	CCWWGG	3 cut(s)	351, 660, 1076
Eco24I	GRGCYC	2 cut(s)	266, 878
Eco31I	GGTCTC	1 cut(s)	1338
Eco52I	CGGCCG	2 cut(s)	18, 118
Eco57I	CTGAAG	1 cut(s)	396
Eco72I	CACGTG	3 cut(s)	39, 69, 101
Eco88I	CYCGRG	1 cut(s)	243
Eco91I	GGTNACC	2 cut(s)	445, 1427
EcoO109I	RGGNCCY	1 cut(s)	190
EcoO65I	GGTNACC	2 cut(s)	445, 1427
EcoRI	GAATTC	1 cut(s)	928
EcoRII	CCWGG	1 cut(s)	89
EcoT14I	CCWWGG	3 cut(s)	351, 660, 1076
EcoT22I	ATGCAT	1 cut(s)	1262
EcoT38I	GRGCYC	2 cut(s)	266, 878
ErhI	CCWWGG	3 cut(s)	351, 660, 1076
Esp3I	CGTCTC	1 cut(s)	118
FalI	AAGNNNNNCTT	2 cut(s)	644, 676
FaqI	GGGAC	2 cut(s)	10, 92
Fnu4HI	GCNGC	9 cut(s)	6, 9, 21, 51, 115, 118, 151, 1443, 1467
FokI	GGATG	7 cut(s)	600, 883, 1313, 1315, 1485, 1503, 1550
FriOI	GRGCYC	2 cut(s)	266, 878
Fsp4HI	GCNGC	9 cut(s)	6, 9, 21, 51, 115, 118, 151, 1443, 1467
FspBI	CTAG	4 cut(s)	1133, 1187, 1436, 1586
GlaI	GCGC	2 cut(s)	326, 1450
GluI	GCNGC	9 cut(s)	6, 9, 21, 51, 115, 118, 151, 1443, 1467
GsuI	CTGGAG	2 cut(s)	1140, 1362
HaeII	RGCGCY	1 cut(s)	328
HaeIII	GGCC	7 cut(s)	20, 94, 120, 192, 876, 1383, 1460
HapII	CCGG	1 cut(s)	121
HgaI	GACGC	1 cut(s)	12
HhaI	GCGC	2 cut(s)	327, 1451
Hin6I	GCGC	2 cut(s)	325, 1449
HinP1I	GCGC	2 cut(s)	325, 1449
HincII	GTYRAC	1 cut(s)	1405
HindII	GTYRAC	1 cut(s)	1405
HindIII	AAGCTT	3 cut(s)	522, 774, 1195
HinfI	GANTC	5 cut(s)	310, 802, 986, 1387, 1401
HpaII	CCGG	1 cut(s)	121
HphI	GGTGA	3 cut(s)	457, 965, 1421
Hpy166II	GTNNAC	1 cut(s)	1405
Hpy188I	TCNGA	2 cut(s)	985, 1368
Hpy188III	TCNNGA	7 cut(s)	461, 609, 1071, 1091, 1133, 1391, 1514
Hpy8I	GTNNAC	1 cut(s)	1405
Hpy99I	CGWCG	2 cut(s)	50, 77
HpyAV	CCTTC	2 cut(s)	1106, 1261
HpyCH4III	ACNGT	1 cut(s)	1052
HpyCH4IV	ACGT	3 cut(s)	38, 68, 100
HpyF3I	CTNAG	5 cut(s)	1152, 1224, 1278, 1293, 1392
HpySE526I	ACGT	3 cut(s)	38, 68, 100
HspAI	GCGC	2 cut(s)	325, 1449
Kzo9I	GATC	8 cut(s)	176, 290, 564, 726, 753, 859, 1054, 1582
LmnI	GCTCC	3 cut(s)	1115, 1439, 1566
Lsp1109I	GCAGC	3 cut(s)	162, 1454, 1478
LweI	GCATC	5 cut(s)	454, 754, 1076, 1198, 1359
MaeI	CTAG	4 cut(s)	1133, 1187, 1436, 1586
MaeII	ACGT	3 cut(s)	38, 68, 100
MaeIII	GTNAC	7 cut(s)	64, 96, 427, 445, 1427, 1574, 1588
MalI	GATC	8 cut(s)	178, 292, 566, 728, 755, 861, 1056, 1584
MboI	GATC	8 cut(s)	176, 290, 564, 726, 753, 859, 1054, 1582
MboII	GAAGA	9 cut(s)	246, 389, 406, 624, 695, 748, 763, 808, 1148
MfeI	CAATTG	1 cut(s)	768
MflI	RGATCY	5 cut(s)	176, 290, 753, 859, 1582
MhlI	GDGCHC	3 cut(s)	266, 878, 1036
MlsI	TGGCCA	1 cut(s)	1383
MluCI	AATT	9 cut(s)	380, 552, 623, 675, 699, 768, 880, 928, 1306
MluNI	TGGCCA	1 cut(s)	1383
MlyI	GAGTC	1 cut(s)	1410
MmeI	TCCRAC	4 cut(s)	848, 1082, 1225, 1622
Mox20I	TGGCCA	1 cut(s)	1383
Mph1103I	ATGCAT	1 cut(s)	1262
MroXI	GAANNNNTTC	2 cut(s)	755, 806
MscI	TGGCCA	1 cut(s)	1383
MseI	TTAA	5 cut(s)	161, 330, 1274, 1314, 1350
MslI	CAYNNNNRTG	1 cut(s)	1621
Msp20I	TGGCCA	1 cut(s)	1383
MspA1I	CMGCKG	2 cut(s)	1340, 1445
MspCI	CTTAAG	1 cut(s)	160
MspI	CCGG	1 cut(s)	121
MspR9I	CCNGG	1 cut(s)	91
MunI	CAATTG	1 cut(s)	768
Mva1269I	GAATGC	1 cut(s)	183
MvaI	CCWGG	1 cut(s)	91
MvnI	CGCG	1 cut(s)	23
NcoI	CCATGG	1 cut(s)	351
NdeII	GATC	8 cut(s)	176, 290, 564, 726, 753, 859, 1054, 1582
NlaIV	GGNNCC	3 cut(s)	178, 292, 876
NmuCI	GTSAC	6 cut(s)	64, 96, 427, 445, 1427, 1588
NsiI	ATGCAT	1 cut(s)	1262
NspI	RCATGY	5 cut(s)	61, 1029, 1151, 1206, 1325
NspV	TTCGAA	1 cut(s)	1610
OliI	CACNNNNGTG	1 cut(s)	1621
PaeI	GCATGC	1 cut(s)	1029
PagI	TCATGA	1 cut(s)	460
PaqCI	CACCTGC	1 cut(s)	1440
PciI	ACATGT	1 cut(s)	1202
PctI	GAATGC	1 cut(s)	183
PdmI	GAANNNNTTC	2 cut(s)	755, 806
PfeI	GAWTC	4 cut(s)	310, 802, 986, 1387
PkrI	GCNGC	9 cut(s)	7, 10, 22, 52, 116, 119, 152, 1444, 1468
PleI	GAGTC	1 cut(s)	1409
PmaCI	CACGTG	3 cut(s)	39, 69, 101
PmlI	CACGTG	3 cut(s)	39, 69, 101
PpsI	GAGTC	1 cut(s)	1409
Ppu21I	YACGTR	3 cut(s)	39, 69, 101
PscI	ACATGT	1 cut(s)	1202
PshBI	ATTAAT	1 cut(s)	1314
Psp6I	CCWGG	1 cut(s)	89
PspCI	CACGTG	3 cut(s)	39, 69, 101
PspEI	GGTNACC	2 cut(s)	445, 1427
PspGI	CCWGG	1 cut(s)	89
PspN4I	GGNNCC	3 cut(s)	178, 292, 876
PspOMI	GGGCCC	1 cut(s)	874
PspPI	GGNCC	3 cut(s)	190, 874, 875
PstNI	CAGNNNCTG	2 cut(s)	156, 193
PsuI	RGATCY	5 cut(s)	176, 290, 753, 859, 1582
PvuII	CAGCTG	2 cut(s)	1340, 1445
RsaI	GTAC	2 cut(s)	233, 1473
RsaNI	GTAC	2 cut(s)	232, 1472
RseI	CAYNNNNRTG	1 cut(s)	1621
SaqAI	TTAA	5 cut(s)	161, 330, 1274, 1314, 1350
SatI	GCNGC	9 cut(s)	6, 9, 21, 51, 115, 118, 151, 1443, 1467
Sau3AI	GATC	8 cut(s)	176, 290, 564, 726, 753, 859, 1054, 1582
Sau96I	GGNCC	3 cut(s)	190, 874, 875
ScaI	AGTACT	1 cut(s)	233
SchI	GAGTC	1 cut(s)	1410
ScrFI	CCNGG	1 cut(s)	91
SduI	GDGCHC	3 cut(s)	266, 878, 1036
SfaNI	GCATC	5 cut(s)	454, 754, 1076, 1198, 1359
SfcI	CTRYAG	1 cut(s)	235
SfuI	TTCGAA	1 cut(s)	1610
SmiMI	CAYNNNNRTG	1 cut(s)	1621
SmlI	CTYRAG	2 cut(s)	160, 491
SmoI	CTYRAG	2 cut(s)	160, 491
SphI	GCATGC	1 cut(s)	1029
Sse9I	AATT	9 cut(s)	380, 552, 623, 675, 699, 768, 880, 928, 1306
SsiI	CCGC	7 cut(s)	9, 21, 51, 87, 114, 117, 174
SspMI	CTAG	4 cut(s)	1133, 1187, 1436, 1586
StyD4I	CCNGG	1 cut(s)	89
StyI	CCWWGG	3 cut(s)	351, 660, 1076
TaaI	ACNGT	1 cut(s)	1052
TaiI	ACGT	3 cut(s)	41, 71, 103
TaqI	TCGA	2 cut(s)	313, 1610
TaqII	GACCGA	1 cut(s)	52
TasI	AATT	9 cut(s)	380, 552, 623, 675, 699, 768, 880, 928, 1306
TatI	WGTACW	2 cut(s)	231, 1471
TauI	GCSGC	5 cut(s)	11, 23, 53, 117, 120
TfiI	GAWTC	4 cut(s)	310, 802, 986, 1387
Tru1I	TTAA	5 cut(s)	161, 330, 1274, 1314, 1350
Tru9I	TTAA	5 cut(s)	161, 330, 1274, 1314, 1350
TscAI	CASTG	3 cut(s)	1057, 1458, 1623
TseFI	GTSAC	6 cut(s)	64, 96, 427, 445, 1427, 1588
TseI	GCWGC	4 cut(s)	5, 150, 1442, 1466
Tsp45I	GTSAC	6 cut(s)	64, 96, 427, 445, 1427, 1588
TspDTI	ATGAA	5 cut(s)	616, 764, 777, 815, 1487
TspGWI	ACGGA	1 cut(s)	142
TspRI	CASTG	3 cut(s)	1057, 1458, 1623
Vha464I	CTTAAG	1 cut(s)	160
VspI	ATTAAT	1 cut(s)	1314
XapI	RAATTY	2 cut(s)	623, 928
XbaI	TCTAGA	1 cut(s)	1132
XceI	RCATGY	5 cut(s)	61, 1029, 1151, 1206, 1325
XcmI	CCANNNNNNNNNTGG	1 cut(s)	853
XmnI	GAANNNNTTC	2 cut(s)	755, 806
XspI	CTAG	4 cut(s)	1133, 1187, 1436, 1586
ZrmI	AGTACT	1 cut(s)	233
Zsp2I	ATGCAT	1 cut(s)	1262

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.