MD02G1109600.v1.1
ERF Family

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003454
Physical Location & Seq
Forward (+)
8837621 .. 8838665
1045 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1109600.v1.1.491

Sequence Viewer

Length: 738 bp
ATGCTTTCAGTTGCTGGCGGTCCTGAAATGGCTCAAGACAGCGAATATTTTCAAGCTTCTGCTCTGGTTAACCAACCTTCTCTAATTTCCAAGAGGAACCAACTGAGCCAGCATCTTAGAGAAGGAAAGATTGAAACCCAGGAAACTTTCTCCGGCATAAATGGGGTTCTTTATTACACTCCCCAAATTCTTGAGCAAGCAGGGGTGGCAATTCTCCTATCGGATTTAGGCCTTAGTTCCACCTCTTCATCCATTCTCGTAAGTGCTCTCACAACCCTATTGATGCTTCCGTCTATCGGCATTGCCATGAGACTAATGGATAGTTCTGGCAGAAGGTCGCTGTTGCTAACTACATTACCGGTACTAACTGGCACCCTCCTAGTACTAACTGGCACCCTCCTAGTACTAATTTTCAGACAACTTGTCCATTTGGGTTCCGTTGTCAATGCAACAATTTCTACCATCAGCGTTGTGGTCTACTTCTGCACCTTTGTCATGGGGTTTGGACCAATCCCTAACATCCTTTGCTCGGAGATCTTCCCCACTCGGGTTCGTGGCCTCTGCATTGCAATATGTGCCCTGACATTTTGGATTGAAGACATCATTGGGACTTACACATTGCCTATCATGCTCACCTCGATTGGCCTCGCTGGTGTTTTTGCTATCTATGTTGTTGTGTGCACCATTTCTTGGGTGTTTGTTTTCTTAAAATGCCAGAAACAAAGGGAATGCCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sugar_tr

Protein Analysis

246

Amino Acids

26.53

Weight (kDa)

6.7

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sugar_tr PF00083 48 - 237 7.3e-28 Sugar (and other) transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 371, 392
AccB7I CCANNNNNTGG 1 cut(s) 690
AccI GTMKAC 1 cut(s) 477
AciI CCGC 1 cut(s) 18
AcsI RAATTY 1 cut(s) 186
AfaI GTAC 3 cut(s) 363, 384, 405
AfiI CCNNNNNNNGG 4 cut(s) 296, 529, 547, 690
AgeI ACCGGT 1 cut(s) 358
AgsI TTSAA 3 cut(s) 53, 134, 596
AhdI GACNNNNNGTC 1 cut(s) 422
AjnI CCWGG 1 cut(s) 138
AjuI GAANNNNNNNTTGG 2 cut(s) 588, 620
AluBI AGCT 1 cut(s) 56
AluI AGCT 1 cut(s) 56
Alw21I GWGCWC 2 cut(s) 268, 683
Alw26I GTCTC 1 cut(s) 304
Alw44I GTGCAC 1 cut(s) 679
AlwNI CAGNNNCTG 1 cut(s) 14
Ama87I CYCGRG 1 cut(s) 546
AoxI GGCC 3 cut(s) 229, 556, 643
ApaLI GTGCAC 1 cut(s) 679
ApoI RAATTY 1 cut(s) 186
AsiGI ACCGGT 1 cut(s) 358
Asp700I GAANNNNTTC 1 cut(s) 48
AspS9I GGNCC 2 cut(s) 20, 506
AsuHPI GGTGA 1 cut(s) 625
AvaI CYCGRG 1 cut(s) 546
AvaII GGWCC 2 cut(s) 20, 506
BaeGI GKGCMC 2 cut(s) 580, 683
BanI GGYRCC 2 cut(s) 371, 392
BbsI GAAGAC 1 cut(s) 603
Bbv12I GWGCWC 2 cut(s) 268, 683
BccI CCATC 1 cut(s) 470
BciT130I CCWGG 1 cut(s) 140
BcoDI GTCTC 1 cut(s) 304
BfaI CTAG 2 cut(s) 380, 401
BglII AGATCT 1 cut(s) 534
BmcAI AGTACT 2 cut(s) 384, 405
Bme1390I CCNGG 1 cut(s) 140
Bme18I GGWCC 2 cut(s) 20, 506
BmeRI GACNNNNNGTC 1 cut(s) 422
BmeT110I CYCGRG 1 cut(s) 546
BmgT120I GGNCC 2 cut(s) 20, 506
BmiI GGNNCC 4 cut(s) 98, 373, 394, 436
BmrFI CCNGG 1 cut(s) 140
BmsI GCATC 2 cut(s) 121, 273
BpiI GAAGAC 1 cut(s) 603
BpuEI CTTGAG 2 cut(s) 18, 212
BsaJI CCNNGG 1 cut(s) 138
BsaWI WCCGGW 1 cut(s) 358
Bsc4I CCNNNNNNNGG 4 cut(s) 296, 529, 547, 690
Bse118I RCCGGY 1 cut(s) 358
Bse1I ACTGG 2 cut(s) 373, 394
Bse3DI GCAATG 3 cut(s) 300, 564, 617
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 1 cut(s) 138
BseGI GGATG 2 cut(s) 248, 519
BseLI CCNNNNNNNGG 4 cut(s) 296, 529, 547, 690
BseMI GCAATG 3 cut(s) 300, 564, 617
BseMII CTCAG 1 cut(s) 95
BseNI ACTGG 2 cut(s) 373, 394
BseSI GKGCMC 2 cut(s) 580, 683
BsgI GTGCAG 1 cut(s) 469
BshFI GGCC 3 cut(s) 231, 558, 645
BshNI GGYRCC 2 cut(s) 371, 392
BshTI ACCGGT 1 cut(s) 358
BsiHKAI GWGCWC 2 cut(s) 268, 683
BsiHKCI CYCGRG 1 cut(s) 546
BsiSI CCGG 2 cut(s) 153, 359
BslFI GGGAC 1 cut(s) 622
BslI CCNNNNNNNGG 4 cut(s) 296, 529, 547, 690
BsmAI GTCTC 1 cut(s) 304
BsmFI GGGAC 1 cut(s) 622
BsmI GAATGC 1 cut(s) 734
BsnI GGCC 3 cut(s) 231, 558, 645
BsoBI CYCGRG 1 cut(s) 546
Bsp1286I GDGCHC 3 cut(s) 268, 580, 683
Bsp143I GATC 1 cut(s) 534
BspACI CCGC 1 cut(s) 18
BspANI GGCC 3 cut(s) 231, 558, 645
BspCNI CTCAG 1 cut(s) 96
BspLI GGNNCC 4 cut(s) 98, 373, 394, 436
BspT107I GGYRCC 2 cut(s) 371, 392
BsrDI GCAATG 3 cut(s) 300, 564, 617
BsrFI RCCGGY 1 cut(s) 358
BsrI ACTGG 2 cut(s) 373, 394
BssAI RCCGGY 1 cut(s) 358
BssECI CCNNGG 1 cut(s) 138
BssMI GATC 1 cut(s) 534
Bst2UI CCWGG 1 cut(s) 140
Bst6I CTCTTC 1 cut(s) 250
BstAPI GCANNNNNTGC 1 cut(s) 575
BstC8I GCNNGC 3 cut(s) 16, 110, 198
BstDEI CTNAG 4 cut(s) 104, 116, 233, 735
BstF5I GGATG 2 cut(s) 248, 519
BstKTI GATC 1 cut(s) 537
BstMAI GTCTC 1 cut(s) 304
BstMBI GATC 1 cut(s) 534
BstMWI GCNNNNNNNGC 3 cut(s) 206, 575, 628
BstNI CCWGG 1 cut(s) 140
BstSCI CCNGG 1 cut(s) 138
BstSLI GKGCMC 2 cut(s) 580, 683
BstV2I GAAGAC 1 cut(s) 603
BstX2I RGATCY 1 cut(s) 534
BstYI RGATCY 1 cut(s) 534
BsuRI GGCC 3 cut(s) 231, 558, 645
BtsCI GGATG 2 cut(s) 248, 519
Cac8I GCNNGC 3 cut(s) 16, 110, 198
CaiI CAGNNNCTG 1 cut(s) 14
Cfr10I RCCGGY 1 cut(s) 358
Cfr13I GGNCC 2 cut(s) 20, 506
Csp6I GTAC 3 cut(s) 362, 383, 404
CspAI ACCGGT 1 cut(s) 358
CviAII CATG 3 cut(s) 307, 496, 628
CviJI RGCY 6 cut(s) 32, 56, 108, 231, 558, 645
CviKI_1 RGCY 6 cut(s) 32, 56, 108, 231, 558, 645
CviQI GTAC 3 cut(s) 362, 383, 404
DdeI CTNAG 4 cut(s) 104, 116, 233, 735
DpnI GATC 1 cut(s) 536
DpnII GATC 1 cut(s) 534
DriI GACNNNNNGTC 1 cut(s) 422
Eam1104I CTCTTC 1 cut(s) 250
Eam1105I GACNNNNNGTC 1 cut(s) 422
EarI CTCTTC 1 cut(s) 250
Eco147I AGGCCT 1 cut(s) 231
Eco47I GGWCC 2 cut(s) 20, 506
Eco88I CYCGRG 1 cut(s) 546
EcoRII CCWGG 1 cut(s) 138
FaeI CATG 3 cut(s) 310, 499, 631
FaiI YATR 6 cut(s) 158, 308, 497, 574, 629, 669
FaqI GGGAC 1 cut(s) 622
FatI CATG 3 cut(s) 306, 495, 627
FblI GTMKAC 1 cut(s) 477
FokI GGATG 2 cut(s) 235, 506
FspBI CTAG 2 cut(s) 380, 401
HaeIII GGCC 3 cut(s) 231, 558, 645
HapII CCGG 2 cut(s) 153, 359
Hin1II CATG 3 cut(s) 310, 499, 631
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HindIII AAGCTT 1 cut(s) 54
HpaI GTTAAC 1 cut(s) 70
HpaII CCGG 2 cut(s) 153, 359
HphI GGTGA 1 cut(s) 625
Hpy166II GTNNAC 3 cut(s) 70, 478, 681
Hpy188I TCNGA 3 cut(s) 223, 416, 532
Hpy188III TCNNGA 3 cut(s) 23, 35, 191
Hpy8I GTNNAC 3 cut(s) 70, 478, 681
HpyAV CCTTC 3 cut(s) 87, 116, 327
HpyCH4V TGCA 5 cut(s) 449, 486, 564, 569, 681
HpyF10VI GCNNNNNNNGC 3 cut(s) 206, 575, 628
HpyF3I CTNAG 4 cut(s) 104, 116, 233, 735
Hsp92II CATG 3 cut(s) 310, 499, 631
KspAI GTTAAC 1 cut(s) 70
Kzo9I GATC 1 cut(s) 534
LweI GCATC 2 cut(s) 121, 273
MaeI CTAG 2 cut(s) 380, 401
MalI GATC 1 cut(s) 536
MboI GATC 1 cut(s) 534
MboII GAAGA 3 cut(s) 237, 529, 608
MflI RGATCY 1 cut(s) 534
MhlI GDGCHC 3 cut(s) 268, 580, 683
MluCI AATT 5 cut(s) 84, 186, 210, 408, 453
MnlI CCTC 7 cut(s) 87, 253, 386, 407, 569, 646, 656
MroXI GAANNNNTTC 1 cut(s) 48
MseI TTAA 2 cut(s) 69, 707
MslI CAYNNNNRTG 1 cut(s) 305
MspI CCGG 2 cut(s) 153, 359
MspR9I CCNGG 1 cut(s) 140
Mva1269I GAATGC 1 cut(s) 734
MvaI CCWGG 1 cut(s) 140
MwoI GCNNNNNNNGC 3 cut(s) 206, 575, 628
NdeII GATC 1 cut(s) 534
NlaIII CATG 3 cut(s) 310, 499, 631
NlaIV GGNNCC 4 cut(s) 98, 373, 394, 436
PceI AGGCCT 1 cut(s) 231
PctI GAATGC 1 cut(s) 734
PdmI GAANNNNTTC 1 cut(s) 48
PflMI CCANNNNNTGG 1 cut(s) 690
PinAI ACCGGT 1 cut(s) 358
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 4 cut(s) 98, 373, 394, 436
PspPI GGNCC 2 cut(s) 20, 506
PstNI CAGNNNCTG 1 cut(s) 14
PsuI RGATCY 1 cut(s) 534
RsaI GTAC 3 cut(s) 363, 384, 405
RsaNI GTAC 3 cut(s) 362, 383, 404
RseI CAYNNNNRTG 1 cut(s) 305
SaqAI TTAA 2 cut(s) 69, 707
Sau3AI GATC 1 cut(s) 534
Sau96I GGNCC 2 cut(s) 20, 506
ScaI AGTACT 2 cut(s) 384, 405
ScrFI CCNGG 1 cut(s) 140
SduI GDGCHC 3 cut(s) 268, 580, 683
SetI ASST 6 cut(s) 58, 79, 245, 338, 491, 638
SfaNI GCATC 2 cut(s) 121, 273
SinI GGWCC 2 cut(s) 20, 506
SmiMI CAYNNNNRTG 1 cut(s) 305
SmlI CTYRAG 2 cut(s) 33, 191
SmoI CTYRAG 2 cut(s) 33, 191
Sse9I AATT 5 cut(s) 84, 186, 210, 408, 453
SseBI AGGCCT 1 cut(s) 231
SsiI CCGC 1 cut(s) 18
SspI AATATT 1 cut(s) 47
SspMI CTAG 2 cut(s) 380, 401
StuI AGGCCT 1 cut(s) 231
StyD4I CCNGG 1 cut(s) 138
TaqI TCGA 1 cut(s) 638
TasI AATT 5 cut(s) 84, 186, 210, 408, 453
TatI WGTACW 2 cut(s) 382, 403
Tru1I TTAA 2 cut(s) 69, 707
Tru9I TTAA 2 cut(s) 69, 707
TspDTI ATGAA 1 cut(s) 237
TspGWI ACGGA 2 cut(s) 279, 427
Van91I CCANNNNNTGG 1 cut(s) 690
VneI GTGCAC 1 cut(s) 679
VpaK11BI GGWCC 2 cut(s) 20, 506
XapI RAATTY 1 cut(s) 186
XcmI CCANNNNNNNNNTGG 2 cut(s) 313, 469
XmiI GTMKAC 1 cut(s) 477
XmnI GAANNNNTTC 1 cut(s) 48
XspI CTAG 2 cut(s) 380, 401
ZrmI AGTACT 2 cut(s) 384, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.