MD02G1182100.v1.1

prolyl 4-hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009888
Physical Location & Seq
Forward (+)
16420352 .. 16423910
3559 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1182100.v1.1.491

Sequence Viewer

Length: 882 bp
ATGAGACCCAAAAGCGGTAAAGGGAATTGGAGCTCCAAGTTCAAGTTCAAGGGCAAACTTGGGTTGCCCACTACTTTCATCTTCTGCTCCTTCTTCTTCCTTGCCGGCTTCTATGGCTCCTCCCTCCTCTCTCAGGATGTCGGTGGTGGTGGTGGTAGGCTGAGAGTGAGAGCTAGACTTTTAGAAACAGAGTACACTTCGATGCCTTATGGAGAGACCGGTGACGATTCTTTAACTTCCATTCCCTTTCAGGTCTTGAGTTGGTATCCCCGAGCATTATATTTTCCTAATTTTGCAACTCCGGAGCAATGCGAAAGCATAATTGGCTTGGCAAAGCCACACCTTAAACCATCGACTTTGGCCTTGCGAGAAGGAGAGACAGAGGAGAGCACAAGGGATATACGAACAAGTTCCGGCATGTTTCTTTCCGCTTCTGATGATAAATCCGGGACTCTGGATGCAATTGAGGAAAAGATTGCAAGGGCTACAATGCTTCCCAGGATACATGGAGAGGCATTTAACGTCTTGCGCTATGAGATCGGGCAGAAGTATAATTCTCATTATGATGCATTCCACCCTGACCAATACGGTCCACAGAAGAGCCAAAGGGTTGCATCGTTCTTGTTGTATTTAACTGAAGTTCAAGAAGGCGGTGAAACCATGTTTCCATATGAGAATGGCTCAAACACGGATGGAACCTATGATTTTCGGGAATGTGTTGGCCTGAAAGTGAAGCCACGCAAAGGGGATGGACTTCTGTTTTATTCATTGCTCCCAAATGGTACAATTGATCCGTTAGCGTTACACGGGAGCTGTCCTGTAATCGAAGGGGAAAAATGGGTAGCTACAAAGTGGATCAGGGATCAAGAACAAGAGGATTAG

Protein Analysis

294

Amino Acids

32.77

Weight (kDa)

5.84

Isoelectric Point (pI)

34.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_3 PF13640 174 - 287 2.3e-16 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 301
AciI CCGC 3 cut(s) 15, 429, 651
AclWI GGATC 3 cut(s) 785, 863, 870
AcuI CTGAAG 1 cut(s) 657
AfaI GTAC 2 cut(s) 194, 784
AfiI CCNNNNNNNGG 3 cut(s) 14, 133, 743
AgeI ACCGGT 1 cut(s) 218
AgsI TTSAA 3 cut(s) 43, 49, 644
AjnI CCWGG 1 cut(s) 497
AjuI GAANNNNNNNTTGG 4 cut(s) 29, 61, 306, 338
AluBI AGCT 4 cut(s) 33, 173, 813, 845
AluI AGCT 4 cut(s) 33, 173, 813, 845
Alw21I GWGCWC 2 cut(s) 35, 392
Alw26I GTCTC 2 cut(s) 209, 371
AlwI GGATC 3 cut(s) 785, 863, 870
Ama87I CYCGRG 1 cut(s) 270
Aor13HI TCCGGA 1 cut(s) 301
AoxI GGCC 2 cut(s) 360, 721
AsiGI ACCGGT 1 cut(s) 218
Asp700I GAANNNNTTC 1 cut(s) 409
AspLEI GCGC 1 cut(s) 531
AspS9I GGNCC 1 cut(s) 590
AsuC2I CCSGG 1 cut(s) 448
AsuHPI GGTGA 2 cut(s) 233, 665
AvaI CYCGRG 1 cut(s) 270
AvaII GGWCC 1 cut(s) 590
BanII GRGCYC 1 cut(s) 35
Bbv12I GWGCWC 2 cut(s) 35, 392
BccI CCATC 3 cut(s) 358, 686, 743
BciT130I CCWGG 1 cut(s) 499
BciVI GTATCC 2 cut(s) 276, 495
BcnI CCSGG 1 cut(s) 448
BcoDI GTCTC 2 cut(s) 209, 371
BfaI CTAG 1 cut(s) 174
BfuI GTATCC 2 cut(s) 276, 495
Bme1390I CCNGG 2 cut(s) 448, 499
Bme18I GGWCC 1 cut(s) 590
BmeT110I CYCGRG 1 cut(s) 270
BmgT120I GGNCC 1 cut(s) 590
BmiI GGNNCC 2 cut(s) 118, 697
BmrFI CCNGG 2 cut(s) 448, 499
BmsI GCATC 4 cut(s) 192, 448, 556, 623
BplI GAGNNNNNCTC 2 cut(s) 665, 697
BpuEI CTTGAG 1 cut(s) 277
BpuMI CCSGG 1 cut(s) 448
BsaI GGTCTC 1 cut(s) 209
BsaJI CCNNGG 1 cut(s) 497
BsaWI WCCGGW 2 cut(s) 218, 301
Bsc4I CCNNNNNNNGG 3 cut(s) 14, 133, 743
Bse118I RCCGGY 2 cut(s) 104, 218
Bse3DI GCAATG 2 cut(s) 314, 767
BseAI TCCGGA 1 cut(s) 301
BseBI CCWGG 1 cut(s) 499
BseDI CCNNGG 1 cut(s) 497
BseGI GGATG 4 cut(s) 142, 463, 697, 754
BseLI CCNNNNNNNGG 3 cut(s) 14, 133, 743
BseMI GCAATG 2 cut(s) 314, 767
BseMII CTCAG 2 cut(s) 146, 152
BseRI GAGGAG 3 cut(s) 109, 116, 398
BshFI GGCC 2 cut(s) 362, 723
BshTI ACCGGT 1 cut(s) 218
BsiHKAI GWGCWC 2 cut(s) 35, 392
BsiHKCI CYCGRG 1 cut(s) 270
BsiSI CCGG 5 cut(s) 105, 219, 302, 414, 447
BslFI GGGAC 1 cut(s) 463
BslI CCNNNNNNNGG 3 cut(s) 14, 133, 743
BsmAI GTCTC 2 cut(s) 209, 371
BsmFI GGGAC 1 cut(s) 463
BsmI GAATGC 1 cut(s) 569
BsnI GGCC 2 cut(s) 362, 723
Bso31I GGTCTC 1 cut(s) 209
BsoBI CYCGRG 1 cut(s) 270
Bsp1286I GDGCHC 2 cut(s) 35, 392
Bsp13I TCCGGA 1 cut(s) 301
Bsp143I GATC 4 cut(s) 537, 790, 855, 862
BspACI CCGC 3 cut(s) 15, 429, 651
BspANI GGCC 2 cut(s) 362, 723
BspCNI CTCAG 2 cut(s) 145, 153
BspEI TCCGGA 1 cut(s) 301
BspLI GGNNCC 2 cut(s) 118, 697
BspPI GGATC 3 cut(s) 785, 863, 870
BspQI GCTCTTC 1 cut(s) 593
BspTNI GGTCTC 1 cut(s) 209
BsrDI GCAATG 2 cut(s) 314, 767
BsrFI RCCGGY 2 cut(s) 104, 218
BssAI RCCGGY 2 cut(s) 104, 218
BssECI CCNNGG 1 cut(s) 497
BssMI GATC 4 cut(s) 537, 790, 855, 862
Bst2UI CCWGG 1 cut(s) 499
Bst4CI ACNGT 1 cut(s) 590
Bst6I CTCTTC 1 cut(s) 593
BstC8I GCNNGC 1 cut(s) 106
BstDEI CTNAG 2 cut(s) 132, 161
BstENI CCTNNNNNAGG 1 cut(s) 131
BstF5I GGATG 4 cut(s) 142, 463, 697, 754
BstHHI GCGC 1 cut(s) 531
BstKTI GATC 4 cut(s) 540, 793, 858, 865
BstMAI GTCTC 2 cut(s) 209, 371
BstMBI GATC 4 cut(s) 537, 790, 855, 862
BstMWI GCNNNNNNNGC 2 cut(s) 114, 324
BstNI CCWGG 1 cut(s) 499
BstNSI RCATGY 1 cut(s) 421
BstSCI CCNGG 2 cut(s) 446, 497
BsuI GTATCC 2 cut(s) 276, 495
BsuRI GGCC 2 cut(s) 362, 723
BtsCI GGATG 4 cut(s) 142, 463, 697, 754
Cac8I GCNNGC 1 cut(s) 106
CfoI GCGC 1 cut(s) 531
Cfr10I RCCGGY 2 cut(s) 104, 218
Cfr13I GGNCC 1 cut(s) 590
Csp6I GTAC 2 cut(s) 193, 783
CspAI ACCGGT 1 cut(s) 218
CviAII CATG 3 cut(s) 418, 506, 661
CviQI GTAC 2 cut(s) 193, 783
DdeI CTNAG 2 cut(s) 132, 161
DpnI GATC 4 cut(s) 539, 792, 857, 864
DpnII GATC 4 cut(s) 537, 790, 855, 862
Eam1104I CTCTTC 1 cut(s) 593
EarI CTCTTC 1 cut(s) 593
Ecl136II GAGCTC 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 35
Eco31I GGTCTC 1 cut(s) 209
Eco47I GGWCC 1 cut(s) 590
Eco53kI GAGCTC 1 cut(s) 33
Eco57I CTGAAG 1 cut(s) 657
Eco88I CYCGRG 1 cut(s) 270
EcoICRI GAGCTC 1 cut(s) 33
EcoNI CCTNNNNNAGG 1 cut(s) 131
EcoRII CCWGG 1 cut(s) 497
EcoT22I ATGCAT 1 cut(s) 571
EcoT38I GRGCYC 1 cut(s) 35
FaeI CATG 3 cut(s) 421, 509, 664
FaqI GGGAC 1 cut(s) 463
FatI CATG 3 cut(s) 417, 505, 660
FauNDI CATATG 1 cut(s) 670
FokI GGATG 4 cut(s) 149, 470, 704, 761
FriOI GRGCYC 1 cut(s) 35
FspBI CTAG 1 cut(s) 174
GlaI GCGC 1 cut(s) 530
HaeIII GGCC 2 cut(s) 362, 723
HapII CCGG 5 cut(s) 105, 219, 302, 414, 447
HhaI GCGC 1 cut(s) 531
Hin1II CATG 3 cut(s) 421, 509, 664
Hin6I GCGC 1 cut(s) 529
HinP1I GCGC 1 cut(s) 529
HinfI GANTC 2 cut(s) 227, 451
HpaII CCGG 5 cut(s) 105, 219, 302, 414, 447
HphI GGTGA 2 cut(s) 233, 665
Hpy166II GTNNAC 2 cut(s) 195, 593
Hpy188I TCNGA 1 cut(s) 436
Hpy188III TCNNGA 7 cut(s) 134, 256, 302, 455, 644, 710, 866
Hpy8I GTNNAC 2 cut(s) 195, 593
HpyAV CCTTC 4 cut(s) 100, 365, 641, 821
HpyCH4III ACNGT 1 cut(s) 590
HpyCH4IV ACGT 1 cut(s) 522
HpyCH4V TGCA 5 cut(s) 296, 461, 479, 569, 614
HpyF10VI GCNNNNNNNGC 2 cut(s) 114, 324
HpyF3I CTNAG 2 cut(s) 132, 161
HpySE526I ACGT 1 cut(s) 522
Hsp92II CATG 3 cut(s) 421, 509, 664
HspAI GCGC 1 cut(s) 529
Kpn2I TCCGGA 1 cut(s) 301
KroI GCCGGC 1 cut(s) 104
KroNI GCCGGC 1 cut(s) 106
Kzo9I GATC 4 cut(s) 537, 790, 855, 862
LguI GCTCTTC 1 cut(s) 593
LmnI GCTCC 7 cut(s) 30, 38, 92, 122, 304, 777, 810
LweI GCATC 4 cut(s) 192, 448, 556, 623
MaeI CTAG 1 cut(s) 174
MaeII ACGT 1 cut(s) 522
MaeIII GTNAC 2 cut(s) 221, 801
MalI GATC 4 cut(s) 539, 792, 857, 864
MboI GATC 4 cut(s) 537, 790, 855, 862
MboII GAAGA 4 cut(s) 73, 85, 88, 610
MfeI CAATTG 2 cut(s) 462, 786
MhlI GDGCHC 2 cut(s) 35, 392
MluCI AATT 6 cut(s) 25, 289, 321, 462, 553, 786
MlyI GAGTC 1 cut(s) 445
MnlI CCTC 7 cut(s) 130, 134, 137, 376, 460, 505, 868
Mph1103I ATGCAT 1 cut(s) 571
MroI TCCGGA 1 cut(s) 301
MroNI GCCGGC 1 cut(s) 104
MroXI GAANNNNTTC 1 cut(s) 409
MseI TTAA 4 cut(s) 233, 345, 519, 632
MslI CAYNNNNRTG 2 cut(s) 200, 564
MspI CCGG 5 cut(s) 105, 219, 302, 414, 447
MspR9I CCNGG 2 cut(s) 448, 499
MunI CAATTG 2 cut(s) 462, 786
Mva1269I GAATGC 1 cut(s) 569
MvaI CCWGG 1 cut(s) 499
MwoI GCNNNNNNNGC 2 cut(s) 114, 324
NaeI GCCGGC 1 cut(s) 106
NciI CCSGG 1 cut(s) 448
NdeI CATATG 1 cut(s) 670
NdeII GATC 4 cut(s) 537, 790, 855, 862
NgoMIV GCCGGC 1 cut(s) 104
NlaIII CATG 3 cut(s) 421, 509, 664
NlaIV GGNNCC 2 cut(s) 118, 697
NmuCI GTSAC 1 cut(s) 221
NsiI ATGCAT 1 cut(s) 571
NspI RCATGY 1 cut(s) 421
PciSI GCTCTTC 1 cut(s) 593
PctI GAATGC 1 cut(s) 569
PdiI GCCGGC 1 cut(s) 106
PdmI GAANNNNTTC 1 cut(s) 409
PfeI GAWTC 1 cut(s) 227
PfoI TCCNGGA 1 cut(s) 446
PinAI ACCGGT 1 cut(s) 218
PleI GAGTC 1 cut(s) 445
PpsI GAGTC 1 cut(s) 445
Psp124BI GAGCTC 1 cut(s) 35
Psp6I CCWGG 1 cut(s) 497
PspGI CCWGG 1 cut(s) 497
PspN4I GGNNCC 2 cut(s) 118, 697
PspPI GGNCC 1 cut(s) 590
RsaI GTAC 2 cut(s) 194, 784
RsaNI GTAC 2 cut(s) 193, 783
RseI CAYNNNNRTG 2 cut(s) 200, 564
SacI GAGCTC 1 cut(s) 35
SapI GCTCTTC 1 cut(s) 593
SaqAI TTAA 4 cut(s) 233, 345, 519, 632
Sau3AI GATC 4 cut(s) 537, 790, 855, 862
Sau96I GGNCC 1 cut(s) 590
SchI GAGTC 1 cut(s) 445
ScrFI CCNGG 2 cut(s) 448, 499
SduI GDGCHC 2 cut(s) 35, 392
SetI ASST 8 cut(s) 35, 175, 255, 345, 525, 701, 815, 847
SfaNI GCATC 4 cut(s) 192, 448, 556, 623
SinI GGWCC 1 cut(s) 590
SmiMI CAYNNNNRTG 2 cut(s) 200, 564
SmlI CTYRAG 1 cut(s) 256
SmoI CTYRAG 1 cut(s) 256
Sse9I AATT 6 cut(s) 25, 289, 321, 462, 553, 786
SsiI CCGC 3 cut(s) 15, 429, 651
SspMI CTAG 1 cut(s) 174
SstI GAGCTC 1 cut(s) 35
StyD4I CCNGG 2 cut(s) 446, 497
TaaI ACNGT 1 cut(s) 590
TaiI ACGT 1 cut(s) 525
TaqI TCGA 3 cut(s) 200, 353, 825
TasI AATT 6 cut(s) 25, 289, 321, 462, 553, 786
TatI WGTACW 1 cut(s) 192
TfiI GAWTC 1 cut(s) 227
Tru1I TTAA 4 cut(s) 233, 345, 519, 632
Tru9I TTAA 4 cut(s) 233, 345, 519, 632
TseFI GTSAC 1 cut(s) 221
Tsp45I GTSAC 1 cut(s) 221
TspDTI ATGAA 2 cut(s) 67, 756
TspGWI ACGGA 2 cut(s) 704, 783
VpaK11BI GGWCC 1 cut(s) 590
XagI CCTNNNNNAGG 1 cut(s) 131
XceI RCATGY 1 cut(s) 421
XmnI GAANNNNTTC 1 cut(s) 409
XspI CTAG 1 cut(s) 174
Zsp2I ATGCAT 1 cut(s) 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.