MD15G1293300.v1.1

prolyl 4-hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009888
Physical Location & Seq
Forward (+)
27177429 .. 27180787
3359 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1293300.v1.1.491

Sequence Viewer

Length: 876 bp
ATGAGACCCAAAAGTGGGAAAGGGAATTGGAGCTCCAAGTTCAAGTTCAAGGGCATACTCGGGTTGCCCACCACTTTGATCTTCTGCTCCTTCTTCTTCCTTGCTGGCTTCTTTGGCTCCTCCCTCCTCTCCCAGCATGTGAGTGGTGGTGGTAATAGGCTGAGAGCGAGACTGTTGGAGACAGAGTACAATTCGATGCCTTATGGCAAGACCGGTGACGATTCTTTAACTTCCATTCCCTTCCAGGTTTTGAGTTGGTATCCGCGAGCATTATATTTTCCTAATTTTGCAACTGCGGAGCAATGCGAAAGCATAATCGGTTTGGCAAAACCACGCCTTAAACCATCGATTTTGGCTTTGCGAGAAGGAGAGACAGAGGAAAGCACAAAGGATATACGAACAAGTTCGGGCGTGTTTCTTTCCGCTTCTGATGATAAATCCGGGACTTTGGATGTGATTGAGGAAAAGATTGCAAGGGCTACAATGCTTCCCAGGACACATGGAGAGGCATTTAACATCTTGCGCTATGAGATCGGGCAGAAGTATAATTCTCATTATGATGCATTCCACCCTGATCAATATGGTCCTCAGAAGAGCCAAAGGGTTGCATCATTCTTGTTGTATTTAAGTGATGTTCAGGAAGGCGGTGAAACCATGTTTCCTTACGAGAATGGCTCAAACACGGATGGAACCTATGATTTTCGGGAATGTGTTGGTTTGAAAGTGAAGCCACGCAAAGGGGATGGACTTCTGTTTTATTCATTGCTTCCAAATGGTACAATTGATATGTTAGCATTACACGGGAGCTGTCCTGTAATCGAAGGGGAAAAATGGGTAGCTACAAAGTGGATCAGGGATCAAGAACAGGAGGATTAG

Protein Analysis

292

Amino Acids

32.55

Weight (kDa)

6.25

Isoelectric Point (pI)

33.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_3 PF13640 172 - 285 3e-16 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 265
AciI CCGC 4 cut(s) 263, 296, 423, 645
AclWI GGATC 2 cut(s) 857, 864
AfaI GTAC 2 cut(s) 188, 778
AfiI CCNNNNNNNGG 3 cut(s) 14, 15, 737
AgeI ACCGGT 1 cut(s) 212
AgsI TTSAA 3 cut(s) 43, 49, 721
AjnI CCWGG 2 cut(s) 243, 491
AjuI GAANNNNNNNTTGG 2 cut(s) 29, 61
AluBI AGCT 3 cut(s) 33, 807, 839
AluI AGCT 3 cut(s) 33, 807, 839
Alw21I GWGCWC 1 cut(s) 35
Alw26I GTCTC 3 cut(s) 163, 173, 365
AlwI GGATC 2 cut(s) 857, 864
Ama87I CYCGRG 1 cut(s) 59
AsiGI ACCGGT 1 cut(s) 212
Asp700I GAANNNNTTC 1 cut(s) 403
AspLEI GCGC 1 cut(s) 525
AspS9I GGNCC 1 cut(s) 584
AsuC2I CCSGG 1 cut(s) 442
AsuHPI GGTGA 2 cut(s) 227, 659
AvaI CYCGRG 1 cut(s) 59
AvaII GGWCC 1 cut(s) 584
BanII GRGCYC 1 cut(s) 35
Bbv12I GWGCWC 1 cut(s) 35
BccI CCATC 3 cut(s) 352, 680, 737
BciT130I CCWGG 2 cut(s) 245, 493
BciVI GTATCC 1 cut(s) 270
BclI TGATCA 1 cut(s) 574
BcnI CCSGG 1 cut(s) 442
BcoDI GTCTC 3 cut(s) 163, 173, 365
BfuI GTATCC 1 cut(s) 270
Bme1390I CCNGG 3 cut(s) 245, 442, 493
Bme18I GGWCC 1 cut(s) 584
BmeT110I CYCGRG 1 cut(s) 59
BmgT120I GGNCC 1 cut(s) 584
BmiI GGNNCC 2 cut(s) 118, 691
BmrFI CCNGG 3 cut(s) 245, 442, 493
BmsI GCATC 3 cut(s) 186, 550, 617
BplI GAGNNNNNCTC 2 cut(s) 659, 691
BpuMI CCSGG 1 cut(s) 442
Bsa29I ATCGAT 1 cut(s) 347
BsaJI CCNNGG 1 cut(s) 491
BsaWI WCCGGW 1 cut(s) 212
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 3 cut(s) 14, 15, 737
Bse118I RCCGGY 1 cut(s) 212
Bse3DI GCAATG 2 cut(s) 308, 761
BseBI CCWGG 2 cut(s) 245, 493
BseCI ATCGAT 1 cut(s) 347
BseDI CCNNGG 1 cut(s) 491
BseGI GGATG 3 cut(s) 457, 691, 748
BseLI CCNNNNNNNGG 3 cut(s) 14, 15, 737
BseMI GCAATG 2 cut(s) 308, 761
BseMII CTCAG 2 cut(s) 152, 602
BseRI GAGGAG 2 cut(s) 109, 116
BseYI CCCAGC 1 cut(s) 132
Bsh1236I CGCG 1 cut(s) 265
BshTI ACCGGT 1 cut(s) 212
BshVI ATCGAT 1 cut(s) 347
BsiHKAI GWGCWC 1 cut(s) 35
BsiHKCI CYCGRG 1 cut(s) 59
BsiSI CCGG 2 cut(s) 213, 441
BslFI GGGAC 1 cut(s) 457
BslI CCNNNNNNNGG 3 cut(s) 14, 15, 737
BsmAI GTCTC 3 cut(s) 163, 173, 365
BsmFI GGGAC 1 cut(s) 457
BsmI GAATGC 1 cut(s) 563
BsoBI CYCGRG 1 cut(s) 59
Bsp1286I GDGCHC 1 cut(s) 35
Bsp143I GATC 5 cut(s) 78, 531, 574, 849, 856
BspACI CCGC 4 cut(s) 263, 296, 423, 645
BspCNI CTCAG 2 cut(s) 153, 601
BspDI ATCGAT 1 cut(s) 347
BspFNI CGCG 1 cut(s) 265
BspLI GGNNCC 2 cut(s) 118, 691
BspPI GGATC 2 cut(s) 857, 864
BspQI GCTCTTC 1 cut(s) 587
BsrDI GCAATG 2 cut(s) 308, 761
BsrFI RCCGGY 1 cut(s) 212
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 491
BssMI GATC 5 cut(s) 78, 531, 574, 849, 856
Bst2UI CCWGG 2 cut(s) 245, 493
Bst4CI ACNGT 1 cut(s) 174
Bst6I CTCTTC 1 cut(s) 587
BstC8I GCNNGC 2 cut(s) 106, 267
BstDEI CTNAG 2 cut(s) 161, 588
BstF5I GGATG 3 cut(s) 457, 691, 748
BstFNI CGCG 1 cut(s) 265
BstHHI GCGC 1 cut(s) 525
BstKTI GATC 5 cut(s) 81, 534, 577, 852, 859
BstMAI GTCTC 3 cut(s) 163, 173, 365
BstMBI GATC 5 cut(s) 78, 531, 574, 849, 856
BstMWI GCNNNNNNNGC 1 cut(s) 114
BstNI CCWGG 2 cut(s) 245, 493
BstNSI RCATGY 1 cut(s) 140
BstSCI CCNGG 3 cut(s) 243, 440, 491
BstUI CGCG 1 cut(s) 265
Bsu15I ATCGAT 1 cut(s) 347
BsuI GTATCC 1 cut(s) 270
BsuTUI ATCGAT 1 cut(s) 347
BtsCI GGATG 3 cut(s) 457, 691, 748
Cac8I GCNNGC 2 cut(s) 106, 267
CfoI GCGC 1 cut(s) 525
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 1 cut(s) 584
ClaI ATCGAT 1 cut(s) 347
Csp6I GTAC 2 cut(s) 187, 777
CspAI ACCGGT 1 cut(s) 212
CviAII CATG 3 cut(s) 137, 500, 655
CviQI GTAC 2 cut(s) 187, 777
DdeI CTNAG 2 cut(s) 161, 588
DpnI GATC 5 cut(s) 80, 533, 576, 851, 858
DpnII GATC 5 cut(s) 78, 531, 574, 849, 856
Eam1104I CTCTTC 1 cut(s) 587
EarI CTCTTC 1 cut(s) 587
Ecl136II GAGCTC 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 35
Eco47I GGWCC 1 cut(s) 584
Eco53kI GAGCTC 1 cut(s) 33
Eco88I CYCGRG 1 cut(s) 59
EcoICRI GAGCTC 1 cut(s) 33
EcoRII CCWGG 2 cut(s) 243, 491
EcoT22I ATGCAT 1 cut(s) 565
EcoT38I GRGCYC 1 cut(s) 35
FaeI CATG 3 cut(s) 140, 503, 658
FaqI GGGAC 1 cut(s) 457
FatI CATG 3 cut(s) 136, 499, 654
FbaI TGATCA 1 cut(s) 574
FokI GGATG 3 cut(s) 464, 698, 755
FriOI GRGCYC 1 cut(s) 35
GlaI GCGC 1 cut(s) 524
GsaI CCCAGC 1 cut(s) 136
HapII CCGG 2 cut(s) 213, 441
HhaI GCGC 1 cut(s) 525
Hin1II CATG 3 cut(s) 140, 503, 658
Hin6I GCGC 1 cut(s) 523
HinP1I GCGC 1 cut(s) 523
HinfI GANTC 1 cut(s) 221
HpaII CCGG 2 cut(s) 213, 441
HphI GGTGA 2 cut(s) 227, 659
Hpy188I TCNGA 2 cut(s) 430, 591
Hpy188III TCNNGA 3 cut(s) 638, 704, 860
HpyAV CCTTC 5 cut(s) 100, 250, 359, 635, 815
HpyCH4III ACNGT 1 cut(s) 174
HpyCH4V TGCA 4 cut(s) 290, 473, 563, 608
HpyF10VI GCNNNNNNNGC 1 cut(s) 114
HpyF3I CTNAG 2 cut(s) 161, 588
Hsp92II CATG 3 cut(s) 140, 503, 658
HspAI GCGC 1 cut(s) 523
Ksp22I TGATCA 1 cut(s) 574
Kzo9I GATC 5 cut(s) 78, 531, 574, 849, 856
LguI GCTCTTC 1 cut(s) 587
LmnI GCTCC 6 cut(s) 30, 38, 92, 122, 298, 804
LweI GCATC 3 cut(s) 186, 550, 617
MaeIII GTNAC 1 cut(s) 215
MalI GATC 5 cut(s) 80, 533, 576, 851, 858
MboI GATC 5 cut(s) 78, 531, 574, 849, 856
MboII GAAGA 4 cut(s) 73, 85, 88, 604
MfeI CAATTG 1 cut(s) 780
MhlI GDGCHC 1 cut(s) 35
MluCI AATT 5 cut(s) 25, 190, 283, 547, 780
MmeI TCCRAC 1 cut(s) 156
MnlI CCTC 8 cut(s) 130, 134, 137, 370, 454, 499, 597, 862
Mph1103I ATGCAT 1 cut(s) 565
MroXI GAANNNNTTC 1 cut(s) 403
MseI TTAA 4 cut(s) 227, 339, 513, 626
MslI CAYNNNNRTG 2 cut(s) 141, 558
MspI CCGG 2 cut(s) 213, 441
MspR9I CCNGG 3 cut(s) 245, 442, 493
MunI CAATTG 1 cut(s) 780
Mva1269I GAATGC 1 cut(s) 563
MvaI CCWGG 2 cut(s) 245, 493
MvnI CGCG 1 cut(s) 265
MwoI GCNNNNNNNGC 1 cut(s) 114
NciI CCSGG 1 cut(s) 442
NdeII GATC 5 cut(s) 78, 531, 574, 849, 856
NlaIII CATG 3 cut(s) 140, 503, 658
NlaIV GGNNCC 2 cut(s) 118, 691
NmuCI GTSAC 1 cut(s) 215
NsiI ATGCAT 1 cut(s) 565
NspI RCATGY 1 cut(s) 140
PciSI GCTCTTC 1 cut(s) 587
PctI GAATGC 1 cut(s) 563
PdmI GAANNNNTTC 1 cut(s) 403
PfeI GAWTC 1 cut(s) 221
PfoI TCCNGGA 1 cut(s) 440
PinAI ACCGGT 1 cut(s) 212
Psp124BI GAGCTC 1 cut(s) 35
Psp6I CCWGG 2 cut(s) 243, 491
PspFI CCCAGC 1 cut(s) 132
PspGI CCWGG 2 cut(s) 243, 491
PspN4I GGNNCC 2 cut(s) 118, 691
PspPI GGNCC 1 cut(s) 584
RsaI GTAC 2 cut(s) 188, 778
RsaNI GTAC 2 cut(s) 187, 777
RseI CAYNNNNRTG 2 cut(s) 141, 558
SacI GAGCTC 1 cut(s) 35
SapI GCTCTTC 1 cut(s) 587
SaqAI TTAA 4 cut(s) 227, 339, 513, 626
Sau3AI GATC 5 cut(s) 78, 531, 574, 849, 856
Sau96I GGNCC 1 cut(s) 584
ScrFI CCNGG 3 cut(s) 245, 442, 493
SduI GDGCHC 1 cut(s) 35
SetI ASST 5 cut(s) 35, 249, 695, 809, 841
SfaNI GCATC 3 cut(s) 186, 550, 617
SinI GGWCC 1 cut(s) 584
SmiMI CAYNNNNRTG 2 cut(s) 141, 558
Sse9I AATT 5 cut(s) 25, 190, 283, 547, 780
SsiI CCGC 4 cut(s) 263, 296, 423, 645
SstI GAGCTC 1 cut(s) 35
StyD4I CCNGG 3 cut(s) 243, 440, 491
TaaI ACNGT 1 cut(s) 174
TaqI TCGA 3 cut(s) 194, 347, 819
TasI AATT 5 cut(s) 25, 190, 283, 547, 780
TatI WGTACW 1 cut(s) 186
TfiI GAWTC 1 cut(s) 221
Tru1I TTAA 4 cut(s) 227, 339, 513, 626
Tru9I TTAA 4 cut(s) 227, 339, 513, 626
TseFI GTSAC 1 cut(s) 215
Tsp45I GTSAC 1 cut(s) 215
TspDTI ATGAA 1 cut(s) 750
TspGWI ACGGA 1 cut(s) 698
VpaK11BI GGWCC 1 cut(s) 584
XceI RCATGY 1 cut(s) 140
XcmI CCANNNNNNNNNTGG 1 cut(s) 140
XmnI GAANNNNTTC 1 cut(s) 403
Zsp2I ATGCAT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.