Rw2G015300

prolyl 4-hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0009888
Physical Location & Seq
Forward (+)
17620361 .. 17622652
2292 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G015300.1

Sequence Viewer

Length: 876 bp
ATGAGACCCAAATCCCTGAAAGGGAATTGGAGCTCCAGGTTGAAGTTCAAGGCCAAGCTACGATTACCCACCGTCCTCGTTTTCTGCTCTTTCTTCTTCCTCGCCGGTTTCTTCATCTCCTCTCTTTTCTCTCAGGATGTAGATGGTGGTGGCAATACACCCAGAACGAGGTTGCTGGAAACAGAGTACGAAACGCTGCCGTTTGGGGAGACAGGAGACGAGTCTTTAACTTCAATTCCCTTCCAGGTGTTGAGCTGGTATCCGCGAGCATTATATTTTCCTAATTTTGCTTCTGCGGAGCAATGTGAGACTATAATCGATTTGGCAAAGCCGGCCCTTAAACCCTCGACCTTGGCTTTGAGAGAGGGAGAGACTGAGGAGAACACACAGGGAATTAGAACAAGTTCTGGGATGTTTCTTTCGGCTTATCAAGATAAGTCTGGGACTTTGGATGTCATTGAGGAAAAGATTGCAAGAGCAACGATGATTCCCAGGACCCATGGAGAGGCATTCAACGTCTTGCGTTATGAGATTGGGCAGAAGTATAATTCTCATTATGATGCATTCCACCCGGAGGAATATGGTCCACAGACAAGCCAAAGGGTTGCATCATTCCTGTTGTATTTAACTGATGTTGAAGAAGGTGGGGAAACAATGTTTCCATATGAGAGTGGCTTGAACCTGGATGGAAAACATGATTCTCAGGAATGTATGGGTTTGAGAGTGAAGCCGCGCAGAGGAGATGGCCTTCTGTTTTATTCATTGTTCCCTAACGGTTCCATTGATCCTTTATCAATACATGGGAGTTGTCCTGTAATCAAAGGCGAAAAATGGGTGGCTACAAAGTGGATGAGGGATCAAGTACAAGAGGAATGA

Protein Analysis

291

Amino Acids

32.83

Weight (kDa)

5.42

Isoelectric Point (pI)

37.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_3 PF13640 172 - 285 2.3e-16 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 265, 733
AciI CCGC 3 cut(s) 263, 296, 731
AclWI GGATC 2 cut(s) 779, 864
AfaI GTAC 2 cut(s) 188, 864
AfiI CCNNNNNNNGG 5 cut(s) 21, 168, 505, 574, 737
AgsI TTSAA 6 cut(s) 43, 49, 234, 514, 638, 679
AjnI CCWGG 4 cut(s) 35, 243, 491, 681
AluBI AGCT 3 cut(s) 33, 58, 255
AluI AGCT 3 cut(s) 33, 58, 255
Alw21I GWGCWC 1 cut(s) 35
Alw26I GTCTC 4 cut(s) 203, 210, 302, 365
AlwI GGATC 2 cut(s) 779, 864
AoxI GGCC 3 cut(s) 51, 333, 745
ApeKI GCWGC 1 cut(s) 196
ArsI GACNNNNNNTTYG 2 cut(s) 340, 372
Asp700I GAANNNNTTC 1 cut(s) 403
AspLEI GCGC 1 cut(s) 735
AspS9I GGNCC 3 cut(s) 334, 495, 584
AsuC2I CCSGG 1 cut(s) 572
AvaII GGWCC 2 cut(s) 495, 584
BanII GRGCYC 1 cut(s) 35
Bbv12I GWGCWC 1 cut(s) 35
BbvI GCAGC 1 cut(s) 183
BccI CCATC 3 cut(s) 137, 680, 737
BceAI ACGGC 1 cut(s) 184
BciT130I CCWGG 4 cut(s) 37, 245, 493, 683
BciVI GTATCC 1 cut(s) 270
BcnI CCSGG 1 cut(s) 572
BcoDI GTCTC 4 cut(s) 203, 210, 302, 365
BfuI GTATCC 1 cut(s) 270
BisI GCNGC 2 cut(s) 197, 731
BlsI GCNGC 2 cut(s) 198, 732
Bme1390I CCNGG 5 cut(s) 37, 245, 493, 572, 683
Bme18I GGWCC 2 cut(s) 495, 584
BmgT120I GGNCC 3 cut(s) 334, 495, 584
BmiI GGNNCC 2 cut(s) 497, 778
BmrFI CCNGG 5 cut(s) 37, 245, 493, 572, 683
BmsI GCATC 2 cut(s) 550, 617
BpmI CTGGAG 1 cut(s) 19
BpuMI CCSGG 1 cut(s) 572
Bsa29I ATCGAT 1 cut(s) 318
BsaJI CCNNGG 3 cut(s) 351, 491, 499
Bsc4I CCNNNNNNNGG 5 cut(s) 21, 168, 505, 574, 737
Bse118I RCCGGY 2 cut(s) 104, 331
Bse3DI GCAATG 1 cut(s) 308
BseBI CCWGG 4 cut(s) 37, 245, 493, 683
BseCI ATCGAT 1 cut(s) 318
BseDI CCNNGG 3 cut(s) 351, 491, 499
BseGI GGATG 5 cut(s) 142, 417, 457, 691, 855
BseLI CCNNNNNNNGG 5 cut(s) 21, 168, 505, 574, 737
BseMI GCAATG 1 cut(s) 308
BseMII CTCAG 3 cut(s) 146, 366, 716
BseRI GAGGAG 3 cut(s) 109, 392, 753
BseXI GCAGC 1 cut(s) 183
Bsh1236I CGCG 2 cut(s) 265, 733
BshFI GGCC 3 cut(s) 53, 335, 747
BshVI ATCGAT 1 cut(s) 318
BsiHKAI GWGCWC 1 cut(s) 35
BsiSI CCGG 3 cut(s) 105, 332, 572
BslFI GGGAC 1 cut(s) 457
BslI CCNNNNNNNGG 5 cut(s) 21, 168, 505, 574, 737
BsmAI GTCTC 4 cut(s) 203, 210, 302, 365
BsmBI CGTCTC 1 cut(s) 210
BsmFI GGGAC 1 cut(s) 457
BsmI GAATGC 2 cut(s) 509, 563
BsnI GGCC 3 cut(s) 53, 335, 747
Bsp1286I GDGCHC 1 cut(s) 35
Bsp143I GATC 2 cut(s) 784, 856
Bsp19I CCATGG 1 cut(s) 499
BspACI CCGC 3 cut(s) 263, 296, 731
BspANI GGCC 3 cut(s) 53, 335, 747
BspCNI CTCAG 3 cut(s) 145, 367, 715
BspDI ATCGAT 1 cut(s) 318
BspFNI CGCG 2 cut(s) 265, 733
BspLI GGNNCC 2 cut(s) 497, 778
BspPI GGATC 2 cut(s) 779, 864
BsrDI GCAATG 1 cut(s) 308
BsrFI RCCGGY 2 cut(s) 104, 331
BssAI RCCGGY 2 cut(s) 104, 331
BssECI CCNNGG 3 cut(s) 351, 491, 499
BssMI GATC 2 cut(s) 784, 856
BssT1I CCWWGG 2 cut(s) 351, 499
Bst2UI CCWGG 4 cut(s) 37, 245, 493, 683
Bst4CI ACNGT 2 cut(s) 73, 776
BstC8I GCNNGC 2 cut(s) 267, 333
BstDEI CTNAG 3 cut(s) 132, 375, 702
BstDSI CCRYGG 1 cut(s) 499
BstF5I GGATG 5 cut(s) 142, 417, 457, 691, 855
BstFNI CGCG 2 cut(s) 265, 733
BstHHI GCGC 1 cut(s) 735
BstKTI GATC 2 cut(s) 787, 859
BstMAI GTCTC 4 cut(s) 203, 210, 302, 365
BstMBI GATC 2 cut(s) 784, 856
BstMWI GCNNNNNNNGC 1 cut(s) 332
BstNI CCWGG 4 cut(s) 37, 245, 493, 683
BstSCI CCNGG 5 cut(s) 35, 243, 491, 570, 681
BstUI CGCG 2 cut(s) 265, 733
BstV1I GCAGC 1 cut(s) 183
Bsu15I ATCGAT 1 cut(s) 318
BsuI GTATCC 1 cut(s) 270
BsuRI GGCC 3 cut(s) 53, 335, 747
BsuTUI ATCGAT 1 cut(s) 318
BtgI CCRYGG 1 cut(s) 499
BtsCI GGATG 5 cut(s) 142, 417, 457, 691, 855
Cac8I GCNNGC 2 cut(s) 267, 333
CfoI GCGC 1 cut(s) 735
Cfr10I RCCGGY 2 cut(s) 104, 331
Cfr13I GGNCC 3 cut(s) 334, 495, 584
ClaI ATCGAT 1 cut(s) 318
Csp6I GTAC 2 cut(s) 187, 863
CviAII CATG 3 cut(s) 500, 695, 800
CviQI GTAC 2 cut(s) 187, 863
DdeI CTNAG 3 cut(s) 132, 375, 702
DpnI GATC 2 cut(s) 786, 858
DpnII GATC 2 cut(s) 784, 856
Ecl136II GAGCTC 1 cut(s) 33
Eco130I CCWWGG 2 cut(s) 351, 499
Eco24I GRGCYC 1 cut(s) 35
Eco47I GGWCC 2 cut(s) 495, 584
Eco53kI GAGCTC 1 cut(s) 33
EcoICRI GAGCTC 1 cut(s) 33
EcoO109I RGGNCCY 1 cut(s) 495
EcoRII CCWGG 4 cut(s) 35, 243, 491, 681
EcoT14I CCWWGG 2 cut(s) 351, 499
EcoT22I ATGCAT 1 cut(s) 565
EcoT38I GRGCYC 1 cut(s) 35
ErhI CCWWGG 2 cut(s) 351, 499
Esp3I CGTCTC 1 cut(s) 210
FaeI CATG 3 cut(s) 503, 698, 803
FaqI GGGAC 1 cut(s) 457
FatI CATG 3 cut(s) 499, 694, 799
FauNDI CATATG 1 cut(s) 664
Fnu4HI GCNGC 2 cut(s) 197, 731
FokI GGATG 5 cut(s) 149, 424, 464, 698, 862
FriOI GRGCYC 1 cut(s) 35
Fsp4HI GCNGC 2 cut(s) 197, 731
GlaI GCGC 1 cut(s) 734
GluI GCNGC 2 cut(s) 197, 731
GsuI CTGGAG 1 cut(s) 19
HaeIII GGCC 3 cut(s) 53, 335, 747
HapII CCGG 3 cut(s) 105, 332, 572
HhaI GCGC 1 cut(s) 735
Hin1II CATG 3 cut(s) 503, 698, 803
Hin6I GCGC 1 cut(s) 733
HinP1I GCGC 1 cut(s) 733
HinfI GANTC 3 cut(s) 221, 487, 698
HpaII CCGG 3 cut(s) 105, 332, 572
Hpy166II GTNNAC 1 cut(s) 587
Hpy188III TCNNGA 3 cut(s) 134, 431, 704
Hpy8I GTNNAC 1 cut(s) 587
HpyAV CCTTC 3 cut(s) 250, 635, 758
HpyCH4III ACNGT 2 cut(s) 73, 776
HpyCH4IV ACGT 1 cut(s) 516
HpyCH4V TGCA 3 cut(s) 473, 563, 608
HpyF10VI GCNNNNNNNGC 1 cut(s) 332
HpyF3I CTNAG 3 cut(s) 132, 375, 702
HpySE526I ACGT 1 cut(s) 516
Hsp92II CATG 3 cut(s) 503, 698, 803
HspAI GCGC 1 cut(s) 733
KroI GCCGGC 1 cut(s) 331
KroNI GCCGGC 1 cut(s) 333
Kzo9I GATC 2 cut(s) 784, 856
LmnI GCTCC 3 cut(s) 30, 38, 298
Lsp1109I GCAGC 1 cut(s) 183
LweI GCATC 2 cut(s) 550, 617
MaeII ACGT 1 cut(s) 516
MalI GATC 2 cut(s) 786, 858
MboI GATC 2 cut(s) 784, 856
MboII GAAGA 4 cut(s) 85, 88, 103, 650
MhlI GDGCHC 1 cut(s) 35
MluCI AATT 5 cut(s) 25, 234, 283, 393, 547
MlyI GAGTC 1 cut(s) 230
Mph1103I ATGCAT 1 cut(s) 565
MroNI GCCGGC 1 cut(s) 331
MroXI GAANNNNTTC 1 cut(s) 403
MseI TTAA 3 cut(s) 227, 339, 626
MslI CAYNNNNRTG 1 cut(s) 558
MspI CCGG 3 cut(s) 105, 332, 572
MspR9I CCNGG 5 cut(s) 37, 245, 493, 572, 683
Mva1269I GAATGC 2 cut(s) 509, 563
MvaI CCWGG 4 cut(s) 37, 245, 493, 683
MvnI CGCG 2 cut(s) 265, 733
MwoI GCNNNNNNNGC 1 cut(s) 332
NaeI GCCGGC 1 cut(s) 333
NciI CCSGG 1 cut(s) 572
NcoI CCATGG 1 cut(s) 499
NdeI CATATG 1 cut(s) 664
NdeII GATC 2 cut(s) 784, 856
NgoMIV GCCGGC 1 cut(s) 331
NlaIII CATG 3 cut(s) 503, 698, 803
NlaIV GGNNCC 2 cut(s) 497, 778
NsiI ATGCAT 1 cut(s) 565
PctI GAATGC 2 cut(s) 509, 563
PdiI GCCGGC 1 cut(s) 333
PdmI GAANNNNTTC 1 cut(s) 403
PfeI GAWTC 2 cut(s) 487, 698
PkrI GCNGC 2 cut(s) 198, 732
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
PpuMI RGGWCCY 1 cut(s) 495
Psp124BI GAGCTC 1 cut(s) 35
Psp5II RGGWCCY 1 cut(s) 495
Psp6I CCWGG 4 cut(s) 35, 243, 491, 681
PspGI CCWGG 4 cut(s) 35, 243, 491, 681
PspN4I GGNNCC 2 cut(s) 497, 778
PspPI GGNCC 3 cut(s) 334, 495, 584
PspPPI RGGWCCY 1 cut(s) 495
RsaI GTAC 2 cut(s) 188, 864
RsaNI GTAC 2 cut(s) 187, 863
RseI CAYNNNNRTG 1 cut(s) 558
SacI GAGCTC 1 cut(s) 35
SaqAI TTAA 3 cut(s) 227, 339, 626
SatI GCNGC 2 cut(s) 197, 731
Sau3AI GATC 2 cut(s) 784, 856
Sau96I GGNCC 3 cut(s) 334, 495, 584
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 5 cut(s) 37, 245, 493, 572, 683
SduI GDGCHC 1 cut(s) 35
SfaNI GCATC 2 cut(s) 550, 617
SinI GGWCC 2 cut(s) 495, 584
SmiMI CAYNNNNRTG 1 cut(s) 558
Sse9I AATT 5 cut(s) 25, 234, 283, 393, 547
SsiI CCGC 3 cut(s) 263, 296, 731
SstI GAGCTC 1 cut(s) 35
StyD4I CCNGG 5 cut(s) 35, 243, 491, 570, 681
StyI CCWWGG 2 cut(s) 351, 499
TaaI ACNGT 2 cut(s) 73, 776
TaiI ACGT 1 cut(s) 519
TaqI TCGA 2 cut(s) 318, 347
TasI AATT 5 cut(s) 25, 234, 283, 393, 547
TatI WGTACW 1 cut(s) 862
TauI GCSGC 1 cut(s) 733
TfiI GAWTC 2 cut(s) 487, 698
Tru1I TTAA 3 cut(s) 227, 339, 626
Tru9I TTAA 3 cut(s) 227, 339, 626
TseI GCWGC 1 cut(s) 196
TspDTI ATGAA 2 cut(s) 103, 750
VpaK11BI GGWCC 2 cut(s) 495, 584
XmnI GAANNNNTTC 1 cut(s) 403
Zsp2I ATGCAT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.