Rroxscaffold_2G00136400

prolyl 4-hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009888
Physical Location & Seq
Reverse (-)
74112581 .. 74114838
2258 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00136400.1

Sequence Viewer

Length: 894 bp
ATGAGACCCAAATCCCTGAAAGGGAATTGGAGCTCCAGGTTGAAGTTCAAGGCCAAGCTCCGATTACCCACCGTCCTCGTTTTCTGCTCTTTCTTCTTCCTCGCCGGTTTCTTCATCTCCTCTCTTTTCTCTCAGGCAAATAAAGATGTAGATGGTGGTGGCAATACACCCAGAACGAGGTTGCTGGAAACAGAGTACGAAACGCTGCCGTTTGGGGAGACAGGAGACGAGTCTTTATCTTCAGTTCCCTTCCAGGTGTTGAGCTGGTATCCGCGAGCATTATATTTTCCTAATTTTGCTTCTGCGGAGCAATGTGAGACTATAATCGATTTGGCAAAGCCGGACCTTAAACCCTCGACCTTGGCTTTGAGAGCGGGAGAGACTGAGGAGAACACAAAGGGAATTAGAACAAGTTCTGGGATGTTTCTTTCGGCTTATCAAGACAAGTCTGGGACTTTGGATGTCATTGAGGAAAAGATTGCAAGAGCAACAATGATTCCCAGGACCCATGGAGAGGCATTCAACGTCTTGCGTTATGAGATTGGGCAGAAGTATAATTCTCATTATGATGCATTCCACCCGGAGGAATATGGTCCACAGACAAGCCAAAGGGTTGCATCATTCCTGTTGTATTTAACGGATGTTGAAGAAGGCGGGGAAACAATGTTTCCATACGAGGCAAGATTCAGTGGCTTGAACCTGGATGGAAAACATGATTCTCAGGAATGTATGGGTTTGAGGGTGAAGCCGCGCAGAGGAGATGGCCTTCTGTTTTATTCATTGTTCCCTAACGGTTCCATCGATCCGTTATCAATACATGGGAGTTGTCCTGTAATCAAAGGCGAAAAATGGGTGGCTACAAAGTGGATGCGGGATCAAGTACAAGAGGAATGA

Protein Analysis

297

Amino Acids

33.48

Weight (kDa)

5.85

Isoelectric Point (pI)

37.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_3 PF13640 175 - 291 3.3e-16 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 374
AccII CGCG 2 cut(s) 274, 751
AciI CCGC 6 cut(s) 272, 305, 374, 654, 749, 871
AclWI GGATC 2 cut(s) 797, 882
AcuI CTGAAG 1 cut(s) 225
AfaI GTAC 2 cut(s) 197, 882
AfiI CCNNNNNNNGG 5 cut(s) 21, 177, 514, 583, 755
AgsI TTSAA 5 cut(s) 43, 49, 523, 647, 697
AjnI CCWGG 4 cut(s) 35, 252, 500, 699
AluBI AGCT 3 cut(s) 33, 58, 264
AluI AGCT 3 cut(s) 33, 58, 264
Alw21I GWGCWC 1 cut(s) 35
Alw26I GTCTC 4 cut(s) 212, 219, 311, 374
AlwI GGATC 2 cut(s) 797, 882
AoxI GGCC 2 cut(s) 51, 763
ApeKI GCWGC 1 cut(s) 205
ArsI GACNNNNNNTTYG 2 cut(s) 349, 381
Asp700I GAANNNNTTC 1 cut(s) 412
AspLEI GCGC 1 cut(s) 753
AspS9I GGNCC 3 cut(s) 343, 504, 593
AsuC2I CCSGG 1 cut(s) 581
AsuHPI GGTGA 1 cut(s) 754
AvaII GGWCC 3 cut(s) 343, 504, 593
BanII GRGCYC 1 cut(s) 35
Bbv12I GWGCWC 1 cut(s) 35
BbvI GCAGC 1 cut(s) 192
BccI CCATC 4 cut(s) 146, 698, 755, 806
BceAI ACGGC 1 cut(s) 193
BciT130I CCWGG 4 cut(s) 37, 254, 502, 701
BciVI GTATCC 1 cut(s) 279
BcnI CCSGG 1 cut(s) 581
BcoDI GTCTC 4 cut(s) 212, 219, 311, 374
BfuI GTATCC 1 cut(s) 279
BisI GCNGC 2 cut(s) 206, 749
BlsI GCNGC 2 cut(s) 207, 750
Bme1390I CCNGG 5 cut(s) 37, 254, 502, 581, 701
Bme18I GGWCC 3 cut(s) 343, 504, 593
BmgT120I GGNCC 3 cut(s) 343, 504, 593
BmiI GGNNCC 2 cut(s) 506, 796
BmrFI CCNGG 5 cut(s) 37, 254, 502, 581, 701
BmsI GCATC 3 cut(s) 559, 626, 858
BpmI CTGGAG 1 cut(s) 19
BpuMI CCSGG 1 cut(s) 581
Bsa29I ATCGAT 2 cut(s) 327, 801
BsaJI CCNNGG 3 cut(s) 360, 500, 508
Bsc4I CCNNNNNNNGG 5 cut(s) 21, 177, 514, 583, 755
Bse118I RCCGGY 1 cut(s) 104
Bse3DI GCAATG 1 cut(s) 317
BseBI CCWGG 4 cut(s) 37, 254, 502, 701
BseCI ATCGAT 2 cut(s) 327, 801
BseDI CCNNGG 3 cut(s) 360, 500, 508
BseGI GGATG 5 cut(s) 426, 466, 646, 709, 873
BseLI CCNNNNNNNGG 5 cut(s) 21, 177, 514, 583, 755
BseMI GCAATG 1 cut(s) 317
BseMII CTCAG 3 cut(s) 146, 375, 734
BseRI GAGGAG 3 cut(s) 109, 401, 771
BseXI GCAGC 1 cut(s) 192
Bsh1236I CGCG 2 cut(s) 274, 751
BshFI GGCC 2 cut(s) 53, 765
BshVI ATCGAT 2 cut(s) 327, 801
BsiHKAI GWGCWC 1 cut(s) 35
BsiSI CCGG 3 cut(s) 105, 341, 581
BslFI GGGAC 1 cut(s) 466
BslI CCNNNNNNNGG 5 cut(s) 21, 177, 514, 583, 755
BsmAI GTCTC 4 cut(s) 212, 219, 311, 374
BsmBI CGTCTC 1 cut(s) 219
BsmFI GGGAC 1 cut(s) 466
BsmI GAATGC 2 cut(s) 518, 572
BsnI GGCC 2 cut(s) 53, 765
Bsp1286I GDGCHC 1 cut(s) 35
Bsp143I GATC 2 cut(s) 802, 874
Bsp19I CCATGG 1 cut(s) 508
BspACI CCGC 6 cut(s) 272, 305, 374, 654, 749, 871
BspANI GGCC 2 cut(s) 53, 765
BspCNI CTCAG 3 cut(s) 145, 376, 733
BspDI ATCGAT 2 cut(s) 327, 801
BspFNI CGCG 2 cut(s) 274, 751
BspLI GGNNCC 2 cut(s) 506, 796
BspPI GGATC 2 cut(s) 797, 882
BsrBI CCGCTC 1 cut(s) 374
BsrDI GCAATG 1 cut(s) 317
BsrFI RCCGGY 1 cut(s) 104
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 3 cut(s) 360, 500, 508
BssMI GATC 2 cut(s) 802, 874
BssT1I CCWWGG 2 cut(s) 360, 508
Bst2UI CCWGG 4 cut(s) 37, 254, 502, 701
Bst4CI ACNGT 2 cut(s) 73, 794
BstC8I GCNNGC 1 cut(s) 276
BstDEI CTNAG 3 cut(s) 132, 384, 720
BstDSI CCRYGG 1 cut(s) 508
BstF5I GGATG 5 cut(s) 426, 466, 646, 709, 873
BstFNI CGCG 2 cut(s) 274, 751
BstHHI GCGC 1 cut(s) 753
BstKTI GATC 2 cut(s) 805, 877
BstMAI GTCTC 4 cut(s) 212, 219, 311, 374
BstMBI GATC 2 cut(s) 802, 874
BstMWI GCNNNNNNNGC 1 cut(s) 371
BstNI CCWGG 4 cut(s) 37, 254, 502, 701
BstSCI CCNGG 5 cut(s) 35, 252, 500, 579, 699
BstUI CGCG 2 cut(s) 274, 751
BstV1I GCAGC 1 cut(s) 192
Bsu15I ATCGAT 2 cut(s) 327, 801
BsuI GTATCC 1 cut(s) 279
BsuRI GGCC 2 cut(s) 53, 765
BsuTUI ATCGAT 2 cut(s) 327, 801
BtgI CCRYGG 1 cut(s) 508
BtsCI GGATG 5 cut(s) 426, 466, 646, 709, 873
BtsIMutI CAGTG 1 cut(s) 694
Cac8I GCNNGC 1 cut(s) 276
CfoI GCGC 1 cut(s) 753
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 3 cut(s) 343, 504, 593
ClaI ATCGAT 2 cut(s) 327, 801
Csp6I GTAC 2 cut(s) 196, 881
CviAII CATG 3 cut(s) 509, 713, 818
CviQI GTAC 2 cut(s) 196, 881
DdeI CTNAG 3 cut(s) 132, 384, 720
DpnI GATC 2 cut(s) 804, 876
DpnII GATC 2 cut(s) 802, 874
Ecl136II GAGCTC 1 cut(s) 33
Eco130I CCWWGG 2 cut(s) 360, 508
Eco24I GRGCYC 1 cut(s) 35
Eco47I GGWCC 3 cut(s) 343, 504, 593
Eco53kI GAGCTC 1 cut(s) 33
Eco57I CTGAAG 1 cut(s) 225
EcoICRI GAGCTC 1 cut(s) 33
EcoO109I RGGNCCY 1 cut(s) 504
EcoRII CCWGG 4 cut(s) 35, 252, 500, 699
EcoT14I CCWWGG 2 cut(s) 360, 508
EcoT22I ATGCAT 1 cut(s) 574
EcoT38I GRGCYC 1 cut(s) 35
ErhI CCWWGG 2 cut(s) 360, 508
Esp3I CGTCTC 1 cut(s) 219
FaeI CATG 3 cut(s) 512, 716, 821
FaqI GGGAC 1 cut(s) 466
FatI CATG 3 cut(s) 508, 712, 817
FauI CCCGC 3 cut(s) 367, 647, 864
Fnu4HI GCNGC 2 cut(s) 206, 749
FokI GGATG 5 cut(s) 433, 473, 653, 716, 880
FriOI GRGCYC 1 cut(s) 35
Fsp4HI GCNGC 2 cut(s) 206, 749
GlaI GCGC 1 cut(s) 752
GluI GCNGC 2 cut(s) 206, 749
GsuI CTGGAG 1 cut(s) 19
HaeIII GGCC 2 cut(s) 53, 765
HapII CCGG 3 cut(s) 105, 341, 581
HhaI GCGC 1 cut(s) 753
Hin1II CATG 3 cut(s) 512, 716, 821
Hin6I GCGC 1 cut(s) 751
HinP1I GCGC 1 cut(s) 751
HinfI GANTC 4 cut(s) 230, 496, 684, 716
HpaII CCGG 3 cut(s) 105, 341, 581
HphI GGTGA 1 cut(s) 754
Hpy166II GTNNAC 1 cut(s) 596
Hpy188I TCNGA 1 cut(s) 62
Hpy188III TCNNGA 2 cut(s) 440, 722
Hpy8I GTNNAC 1 cut(s) 596
HpyAV CCTTC 3 cut(s) 259, 644, 776
HpyCH4III ACNGT 2 cut(s) 73, 794
HpyCH4IV ACGT 1 cut(s) 525
HpyCH4V TGCA 3 cut(s) 482, 572, 617
HpyF10VI GCNNNNNNNGC 1 cut(s) 371
HpyF3I CTNAG 3 cut(s) 132, 384, 720
HpySE526I ACGT 1 cut(s) 525
Hsp92II CATG 3 cut(s) 512, 716, 821
HspAI GCGC 1 cut(s) 751
Kzo9I GATC 2 cut(s) 802, 874
LmnI GCTCC 4 cut(s) 30, 38, 63, 307
Lsp1109I GCAGC 1 cut(s) 192
LweI GCATC 3 cut(s) 559, 626, 858
MaeII ACGT 1 cut(s) 525
MalI GATC 2 cut(s) 804, 876
MbiI CCGCTC 1 cut(s) 374
MboI GATC 2 cut(s) 802, 874
MboII GAAGA 5 cut(s) 85, 88, 103, 231, 659
MhlI GDGCHC 1 cut(s) 35
MluCI AATT 4 cut(s) 25, 292, 402, 556
MlyI GAGTC 1 cut(s) 239
Mph1103I ATGCAT 1 cut(s) 574
MroXI GAANNNNTTC 1 cut(s) 412
MseI TTAA 2 cut(s) 348, 635
MslI CAYNNNNRTG 1 cut(s) 567
MspI CCGG 3 cut(s) 105, 341, 581
MspR9I CCNGG 5 cut(s) 37, 254, 502, 581, 701
Mva1269I GAATGC 2 cut(s) 518, 572
MvaI CCWGG 4 cut(s) 37, 254, 502, 701
MvnI CGCG 2 cut(s) 274, 751
MwoI GCNNNNNNNGC 1 cut(s) 371
NciI CCSGG 1 cut(s) 581
NcoI CCATGG 1 cut(s) 508
NdeII GATC 2 cut(s) 802, 874
NlaIII CATG 3 cut(s) 512, 716, 821
NlaIV GGNNCC 2 cut(s) 506, 796
NsiI ATGCAT 1 cut(s) 574
PcsI WCGNNNNNNNCGW 1 cut(s) 798
PctI GAATGC 2 cut(s) 518, 572
PdmI GAANNNNTTC 1 cut(s) 412
PfeI GAWTC 3 cut(s) 496, 684, 716
PkrI GCNGC 2 cut(s) 207, 750
PleI GAGTC 1 cut(s) 238
PpsI GAGTC 1 cut(s) 238
PpuMI RGGWCCY 1 cut(s) 504
Psp124BI GAGCTC 1 cut(s) 35
Psp5II RGGWCCY 1 cut(s) 504
Psp6I CCWGG 4 cut(s) 35, 252, 500, 699
PspGI CCWGG 4 cut(s) 35, 252, 500, 699
PspN4I GGNNCC 2 cut(s) 506, 796
PspPI GGNCC 3 cut(s) 343, 504, 593
PspPPI RGGWCCY 1 cut(s) 504
RsaI GTAC 2 cut(s) 197, 882
RsaNI GTAC 2 cut(s) 196, 881
RseI CAYNNNNRTG 1 cut(s) 567
SacI GAGCTC 1 cut(s) 35
SaqAI TTAA 2 cut(s) 348, 635
SatI GCNGC 2 cut(s) 206, 749
Sau3AI GATC 2 cut(s) 802, 874
Sau96I GGNCC 3 cut(s) 343, 504, 593
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 5 cut(s) 37, 254, 502, 581, 701
SduI GDGCHC 1 cut(s) 35
SfaNI GCATC 3 cut(s) 559, 626, 858
SinI GGWCC 3 cut(s) 343, 504, 593
SmiMI CAYNNNNRTG 1 cut(s) 567
Sse9I AATT 4 cut(s) 25, 292, 402, 556
SsiI CCGC 6 cut(s) 272, 305, 374, 654, 749, 871
SstI GAGCTC 1 cut(s) 35
StyD4I CCNGG 5 cut(s) 35, 252, 500, 579, 699
StyI CCWWGG 2 cut(s) 360, 508
TaaI ACNGT 2 cut(s) 73, 794
TaiI ACGT 1 cut(s) 528
TaqI TCGA 3 cut(s) 327, 356, 801
TasI AATT 4 cut(s) 25, 292, 402, 556
TatI WGTACW 1 cut(s) 880
TauI GCSGC 1 cut(s) 751
TfiI GAWTC 3 cut(s) 496, 684, 716
Tru1I TTAA 2 cut(s) 348, 635
Tru9I TTAA 2 cut(s) 348, 635
TscAI CASTG 1 cut(s) 694
TseI GCWGC 1 cut(s) 205
TspDTI ATGAA 2 cut(s) 103, 768
TspGWI ACGGA 2 cut(s) 653, 795
TspRI CASTG 1 cut(s) 694
VpaK11BI GGWCC 3 cut(s) 343, 504, 593
XmnI GAANNNNTTC 1 cut(s) 412
Zsp2I ATGCAT 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.