RchiOBHm_Chr2g0107361

prolyl 4-hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0009888
Physical Location & Seq
Forward (+)
18670362 .. 18673135
2774 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48139

Sequence Viewer

Length: 903 bp
ATGAGAATCGACCCAATAATGAGACCCAAATCCCTGAAAGGGAATTGGAGCTCCAGGTTGAAGTTCAAGGCCAAGCTACGATTACCCACCGTCCTCGTTTTCTGCTCTTTCTTCTTCCTCGCCGGTTTCTTCATCTCCTCTCTTTTCTCTCAGGCAAATAAAGATGTAGATGGTGGTGGCAATACACCCAGAACGAGGTTGCTGGAAACAGAGTACGAAACGCTGCCGTTTGGGGAGACTGGAGACGAGTCTTTAACTTCAATTCCCTTCCAGGTGTTGAGCTGGTATCCGCGAGCATTATATTTTCCTAATTTTGCTTCTGCGGAGCAATGTGAGACTATAATCGATTTGGCAAAGCCGGCCCTTAAACCCTCGACCTTGGCTTTGAGAGAGGGAGAGACTGAGGAGAACACAAAGGGAATTAGAACAAGTTCTGGGATGTTTCTTTCGGCTTATCAAGACAAGTCTGGGACTTTGGATGTCATTGAGGAAAAGATTGCAAGAGCAACAATGATTCCCAGGACCCATGGAGAGGCATTCAACGTCTTGCGTTATGAGATTGGGCAGAAGTATAATTCTCATTATGATGCATTCCACCCGGAGGAATATGGTCCACAGACAAGCCAAAGGGTTGCATCATTCCTGTTGTATTTAACTGATGTTGAAGAAGGCGGGGAAACAATGTTTCCATACGAGAGTGGCTTGAACCTGGATGGAAAACATGATTCTCAGGAATGTATGGGTTTGAGAGTGAAGCCGCGTAAAGGAGATGGCCTTCTGTTTTATTCATTGTTCCCTAACGGTTCCATTGACCCTTTATCAATACATGGGAGTTGTCCTGTAATCAAAGGCGAAAAATGGGTGGCTACAAAGTGGATGAGGGATCAAGTACAAGAGGAATGA

Protein Analysis

300

Amino Acids

33.85

Weight (kDa)

5.69

Isoelectric Point (pI)

34.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_3 PF13640 181 - 294 1.6e-16 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 292, 760
AciI CCGC 4 cut(s) 290, 323, 672, 758
AclWI GGATC 1 cut(s) 891
AfaI GTAC 2 cut(s) 215, 891
AfiI CCNNNNNNNGG 5 cut(s) 39, 195, 532, 601, 764
AgsI TTSAA 6 cut(s) 61, 67, 261, 541, 665, 706
AjnI CCWGG 4 cut(s) 53, 270, 518, 708
AluBI AGCT 3 cut(s) 51, 76, 282
AluI AGCT 3 cut(s) 51, 76, 282
Alw21I GWGCWC 1 cut(s) 53
Alw26I GTCTC 5 cut(s) 16, 230, 237, 329, 392
AlwI GGATC 1 cut(s) 891
AoxI GGCC 3 cut(s) 69, 360, 772
ApeKI GCWGC 1 cut(s) 223
ArsI GACNNNNNNTTYG 2 cut(s) 367, 399
Asp700I GAANNNNTTC 1 cut(s) 430
AspS9I GGNCC 3 cut(s) 361, 522, 611
AsuC2I CCSGG 1 cut(s) 599
AvaII GGWCC 2 cut(s) 522, 611
BanII GRGCYC 1 cut(s) 53
Bbv12I GWGCWC 1 cut(s) 53
BbvI GCAGC 1 cut(s) 210
BccI CCATC 3 cut(s) 164, 707, 764
BceAI ACGGC 1 cut(s) 211
BciT130I CCWGG 4 cut(s) 55, 272, 520, 710
BciVI GTATCC 1 cut(s) 297
BcnI CCSGG 1 cut(s) 599
BcoDI GTCTC 5 cut(s) 16, 230, 237, 329, 392
BfuI GTATCC 1 cut(s) 297
BisI GCNGC 2 cut(s) 224, 758
BlsI GCNGC 2 cut(s) 225, 759
Bme1390I CCNGG 5 cut(s) 55, 272, 520, 599, 710
Bme18I GGWCC 2 cut(s) 522, 611
BmgT120I GGNCC 3 cut(s) 361, 522, 611
BmiI GGNNCC 2 cut(s) 524, 805
BmrFI CCNGG 5 cut(s) 55, 272, 520, 599, 710
BmsI GCATC 2 cut(s) 577, 644
BpmI CTGGAG 2 cut(s) 37, 261
BpuMI CCSGG 1 cut(s) 599
Bsa29I ATCGAT 1 cut(s) 345
BsaI GGTCTC 1 cut(s) 16
BsaJI CCNNGG 3 cut(s) 378, 518, 526
Bsc4I CCNNNNNNNGG 5 cut(s) 39, 195, 532, 601, 764
Bse118I RCCGGY 2 cut(s) 122, 358
Bse1I ACTGG 1 cut(s) 244
Bse3DI GCAATG 1 cut(s) 335
BseBI CCWGG 4 cut(s) 55, 272, 520, 710
BseCI ATCGAT 1 cut(s) 345
BseDI CCNNGG 3 cut(s) 378, 518, 526
BseGI GGATG 4 cut(s) 444, 484, 718, 882
BseLI CCNNNNNNNGG 5 cut(s) 39, 195, 532, 601, 764
BseMI GCAATG 1 cut(s) 335
BseMII CTCAG 3 cut(s) 164, 393, 743
BseNI ACTGG 1 cut(s) 244
BseRI GAGGAG 2 cut(s) 127, 419
BseXI GCAGC 1 cut(s) 210
Bsh1236I CGCG 2 cut(s) 292, 760
BshFI GGCC 3 cut(s) 71, 362, 774
BshVI ATCGAT 1 cut(s) 345
BsiHKAI GWGCWC 1 cut(s) 53
BsiSI CCGG 3 cut(s) 123, 359, 599
BslFI GGGAC 1 cut(s) 484
BslI CCNNNNNNNGG 5 cut(s) 39, 195, 532, 601, 764
BsmAI GTCTC 5 cut(s) 16, 230, 237, 329, 392
BsmBI CGTCTC 1 cut(s) 237
BsmFI GGGAC 1 cut(s) 484
BsmI GAATGC 2 cut(s) 536, 590
BsnI GGCC 3 cut(s) 71, 362, 774
Bso31I GGTCTC 1 cut(s) 16
Bsp1286I GDGCHC 1 cut(s) 53
Bsp143I GATC 1 cut(s) 883
Bsp19I CCATGG 1 cut(s) 526
BspACI CCGC 4 cut(s) 290, 323, 672, 758
BspANI GGCC 3 cut(s) 71, 362, 774
BspCNI CTCAG 3 cut(s) 163, 394, 742
BspDI ATCGAT 1 cut(s) 345
BspFNI CGCG 2 cut(s) 292, 760
BspLI GGNNCC 2 cut(s) 524, 805
BspPI GGATC 1 cut(s) 891
BspTNI GGTCTC 1 cut(s) 16
BsrDI GCAATG 1 cut(s) 335
BsrFI RCCGGY 2 cut(s) 122, 358
BsrI ACTGG 1 cut(s) 244
BssAI RCCGGY 2 cut(s) 122, 358
BssECI CCNNGG 3 cut(s) 378, 518, 526
BssMI GATC 1 cut(s) 883
BssT1I CCWWGG 2 cut(s) 378, 526
Bst2UI CCWGG 4 cut(s) 55, 272, 520, 710
Bst4CI ACNGT 2 cut(s) 91, 803
BstC8I GCNNGC 2 cut(s) 294, 360
BstDEI CTNAG 3 cut(s) 150, 402, 729
BstDSI CCRYGG 1 cut(s) 526
BstF5I GGATG 4 cut(s) 444, 484, 718, 882
BstFNI CGCG 2 cut(s) 292, 760
BstKTI GATC 1 cut(s) 886
BstMAI GTCTC 5 cut(s) 16, 230, 237, 329, 392
BstMBI GATC 1 cut(s) 883
BstMWI GCNNNNNNNGC 1 cut(s) 359
BstNI CCWGG 4 cut(s) 55, 272, 520, 710
BstSCI CCNGG 5 cut(s) 53, 270, 518, 597, 708
BstUI CGCG 2 cut(s) 292, 760
BstV1I GCAGC 1 cut(s) 210
Bsu15I ATCGAT 1 cut(s) 345
BsuI GTATCC 1 cut(s) 297
BsuRI GGCC 3 cut(s) 71, 362, 774
BsuTUI ATCGAT 1 cut(s) 345
BtgI CCRYGG 1 cut(s) 526
BtsCI GGATG 4 cut(s) 444, 484, 718, 882
Cac8I GCNNGC 2 cut(s) 294, 360
Cfr10I RCCGGY 2 cut(s) 122, 358
Cfr13I GGNCC 3 cut(s) 361, 522, 611
ClaI ATCGAT 1 cut(s) 345
Csp6I GTAC 2 cut(s) 214, 890
CviAII CATG 3 cut(s) 527, 722, 827
CviQI GTAC 2 cut(s) 214, 890
DdeI CTNAG 3 cut(s) 150, 402, 729
DpnI GATC 1 cut(s) 885
DpnII GATC 1 cut(s) 883
Ecl136II GAGCTC 1 cut(s) 51
Eco130I CCWWGG 2 cut(s) 378, 526
Eco24I GRGCYC 1 cut(s) 53
Eco31I GGTCTC 1 cut(s) 16
Eco47I GGWCC 2 cut(s) 522, 611
Eco53kI GAGCTC 1 cut(s) 51
EcoICRI GAGCTC 1 cut(s) 51
EcoO109I RGGNCCY 1 cut(s) 522
EcoRII CCWGG 4 cut(s) 53, 270, 518, 708
EcoT14I CCWWGG 2 cut(s) 378, 526
EcoT22I ATGCAT 1 cut(s) 592
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 2 cut(s) 378, 526
Esp3I CGTCTC 1 cut(s) 237
FaeI CATG 3 cut(s) 530, 725, 830
FaqI GGGAC 1 cut(s) 484
FatI CATG 3 cut(s) 526, 721, 826
FauI CCCGC 1 cut(s) 665
Fnu4HI GCNGC 2 cut(s) 224, 758
FokI GGATG 4 cut(s) 451, 491, 725, 889
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 2 cut(s) 224, 758
GluI GCNGC 2 cut(s) 224, 758
GsuI CTGGAG 2 cut(s) 37, 261
HaeIII GGCC 3 cut(s) 71, 362, 774
HapII CCGG 3 cut(s) 123, 359, 599
Hin1II CATG 3 cut(s) 530, 725, 830
HinfI GANTC 4 cut(s) 6, 248, 514, 725
HpaII CCGG 3 cut(s) 123, 359, 599
Hpy166II GTNNAC 1 cut(s) 614
Hpy188III TCNNGA 2 cut(s) 458, 731
Hpy8I GTNNAC 1 cut(s) 614
HpyAV CCTTC 3 cut(s) 277, 662, 785
HpyCH4III ACNGT 2 cut(s) 91, 803
HpyCH4IV ACGT 1 cut(s) 543
HpyCH4V TGCA 3 cut(s) 500, 590, 635
HpyF10VI GCNNNNNNNGC 1 cut(s) 359
HpyF3I CTNAG 3 cut(s) 150, 402, 729
HpySE526I ACGT 1 cut(s) 543
Hsp92II CATG 3 cut(s) 530, 725, 830
KroI GCCGGC 1 cut(s) 358
KroNI GCCGGC 1 cut(s) 360
Kzo9I GATC 1 cut(s) 883
LmnI GCTCC 3 cut(s) 48, 56, 325
Lsp1109I GCAGC 1 cut(s) 210
LweI GCATC 2 cut(s) 577, 644
MaeII ACGT 1 cut(s) 543
MalI GATC 1 cut(s) 885
MboI GATC 1 cut(s) 883
MboII GAAGA 4 cut(s) 103, 106, 121, 677
MhlI GDGCHC 1 cut(s) 53
MluCI AATT 5 cut(s) 43, 261, 310, 420, 574
MlyI GAGTC 1 cut(s) 257
Mph1103I ATGCAT 1 cut(s) 592
MroNI GCCGGC 1 cut(s) 358
MroXI GAANNNNTTC 1 cut(s) 430
MseI TTAA 3 cut(s) 254, 366, 653
MslI CAYNNNNRTG 1 cut(s) 585
MspI CCGG 3 cut(s) 123, 359, 599
MspR9I CCNGG 5 cut(s) 55, 272, 520, 599, 710
Mva1269I GAATGC 2 cut(s) 536, 590
MvaI CCWGG 4 cut(s) 55, 272, 520, 710
MvnI CGCG 2 cut(s) 292, 760
MwoI GCNNNNNNNGC 1 cut(s) 359
NaeI GCCGGC 1 cut(s) 360
NciI CCSGG 1 cut(s) 599
NcoI CCATGG 1 cut(s) 526
NdeII GATC 1 cut(s) 883
NgoMIV GCCGGC 1 cut(s) 358
NlaIII CATG 3 cut(s) 530, 725, 830
NlaIV GGNNCC 2 cut(s) 524, 805
NsiI ATGCAT 1 cut(s) 592
PctI GAATGC 2 cut(s) 536, 590
PdiI GCCGGC 1 cut(s) 360
PdmI GAANNNNTTC 1 cut(s) 430
PfeI GAWTC 3 cut(s) 6, 514, 725
PkrI GCNGC 2 cut(s) 225, 759
PleI GAGTC 1 cut(s) 256
PpsI GAGTC 1 cut(s) 256
PpuMI RGGWCCY 1 cut(s) 522
Psp124BI GAGCTC 1 cut(s) 53
Psp5II RGGWCCY 1 cut(s) 522
Psp6I CCWGG 4 cut(s) 53, 270, 518, 708
PspGI CCWGG 4 cut(s) 53, 270, 518, 708
PspN4I GGNNCC 2 cut(s) 524, 805
PspPI GGNCC 3 cut(s) 361, 522, 611
PspPPI RGGWCCY 1 cut(s) 522
RsaI GTAC 2 cut(s) 215, 891
RsaNI GTAC 2 cut(s) 214, 890
RseI CAYNNNNRTG 1 cut(s) 585
SacI GAGCTC 1 cut(s) 53
SaqAI TTAA 3 cut(s) 254, 366, 653
SatI GCNGC 2 cut(s) 224, 758
Sau3AI GATC 1 cut(s) 883
Sau96I GGNCC 3 cut(s) 361, 522, 611
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 5 cut(s) 55, 272, 520, 599, 710
SduI GDGCHC 1 cut(s) 53
SetI ASST 9 cut(s) 53, 59, 78, 200, 276, 284, 380, 546, 711
SfaNI GCATC 2 cut(s) 577, 644
SinI GGWCC 2 cut(s) 522, 611
SmiMI CAYNNNNRTG 1 cut(s) 585
Sse9I AATT 5 cut(s) 43, 261, 310, 420, 574
SsiI CCGC 4 cut(s) 290, 323, 672, 758
SstI GAGCTC 1 cut(s) 53
StyD4I CCNGG 5 cut(s) 53, 270, 518, 597, 708
StyI CCWWGG 2 cut(s) 378, 526
TaaI ACNGT 2 cut(s) 91, 803
TaiI ACGT 1 cut(s) 546
TaqI TCGA 3 cut(s) 9, 345, 374
TasI AATT 5 cut(s) 43, 261, 310, 420, 574
TatI WGTACW 1 cut(s) 889
TauI GCSGC 1 cut(s) 760
TfiI GAWTC 3 cut(s) 6, 514, 725
Tru1I TTAA 3 cut(s) 254, 366, 653
Tru9I TTAA 3 cut(s) 254, 366, 653
TseI GCWGC 1 cut(s) 223
TspDTI ATGAA 2 cut(s) 121, 777
VpaK11BI GGWCC 2 cut(s) 522, 611
XmnI GAANNNNTTC 1 cut(s) 430
Zsp2I ATGCAT 1 cut(s) 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.